CN102311508A - Process for extracting transparent high-acyl gellan gum - Google Patents
Process for extracting transparent high-acyl gellan gum Download PDFInfo
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- CN102311508A CN102311508A CN2010102224117A CN201010222411A CN102311508A CN 102311508 A CN102311508 A CN 102311508A CN 2010102224117 A CN2010102224117 A CN 2010102224117A CN 201010222411 A CN201010222411 A CN 201010222411A CN 102311508 A CN102311508 A CN 102311508A
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- gellan gum
- fermented liquid
- acyl gellan
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Abstract
The invention discloses a new process for extracting transparent high-acyl gellan gum. The process comprises the following steps of: fermenting glucose or cane sugar serving as a carbon source and yeast extract and/or peptone serving as a nitrogen source to obtain fermentation liquor, performing coarse filtration, adding protease and muramidase at the temperature of between 40 and 60 DEG C under the condition that the pH value is 6 to 8, reacting fully, pumping into an ultrafiltration membrane to perform precise filtration, precipitating by using an organic solvent, performing solid-liquid separation, drying and crushing to obtain the transparent high-acyl gellan gum. The process for extracting the transparent high-acyl gellan gum is simple and novel, low in cost and high in yield, and the product is high in transparency, purity and dissolubility.
Description
Technical field
The present invention relates to a kind of extraction process of transparent type high acyl gellan gum.
Background technology
Gelling gum is another Microbial exopolysaccharides that U.S. Kelco company succeeds in developing after XG 550; Past attempts is claimed mixed polysaccharide PS～60; Find the successful report of relevant gelling gum laboratory scale fermentative prodn has just been arranged in late 1970s up to 1982.
The substruction of gelling gum molecule is a main chain, is made up of multiple tetrose unit, and is as shown in Figure 1.The monose of participating in forming has glucose, glucuronic acid and rhamnosyl, mol ratio 2: 1: 1.In the crude form of this polysaccharide, per unit has 1.5 O-carboxyl groups approximately, and wherein existing O-acetyl substituting group has O-glyceroyl substituting group again, and the latter is more than the former, and glyceroyl is positioned at 2 of glucosyl residue that 3-connects into key, and ethanoyl is then on 6.The O-acyl group is easy to removed by alkali, thereby obtains low acyl group product by the gelling gum of crude form.Relative therewith, the gelling gum of crude form is called high acyl group product.
High acyl gellan gum can be brought into play good gel, inborn nature, fixing and conjunctiva effect; High acyl gellan gum has following characteristic simultaneously: be reversible, the hot reversible gel of a kind of shearing; And has fabulous perfume bloom performance; Can be shared with other water-sol, synergy is arranged, splendid figuration effect is arranged with starch, be a kind of elastogel, and can strengthen mouthfeel.Therefore, high acyl gellan gum can be widely used in medicine, chemical industry and the foodstuffs industry, is the favorable substitutes of carrageenin, agar, pectin and other lyophilic colloid, has vast market prospect.
At present; The transparent type high acyl gellan gum has only one family of U.S. CP Kelco company to produce abroad, and domestic also not about the patent report of transparent type high acyl gellan gum, the process for extracting of domestic high acyl gellan gum is: carry out salt precipitation earlier; Carry out organic solvent washing again; Drying and crushing, this technology can not be removed protein residual on the colloid, Mierocrystalline cellulose and pigment etc. fully, and the high acyl gellan gum product transparency that therefore makes is low; Poorly soluble, do not reach the standard of transparent type high acyl gellan gum.
Summary of the invention
The purpose of this invention is to provide a kind of at first with the fermented liquid coarse filtration; Carrying out essence behind the enzymolysis again filters; Make the extraction process of transparent type high acyl gellan gum product then through organic solvent deposit, solid-liquid separation, drying, pulverizing; Thereby overcome low, the poorly soluble shortcoming of transparency in the prior art.
Adopt following technical scheme in order to achieve the above object:
A kind of extraction process of transparent type high acyl gellan gum: will be carbon source at first with glucose or sucrose; With the fermentation of yeast extract paste and/or peptone nitrogenous source and the fermented liquid coarse filtration; 40～60 ℃ of temperature, under the condition of pH value 6～8, after adding proteolytic enzyme and N,O-Diacetylmuramidase fully act on; Pump into ultra-filtration membrane and carry out the essence filtration, make transparent type high acyl gellan gum product through organic solvent deposit, solid-liquid separation, drying, pulverizing then.
Described fermented liquid coarse filtration is the super-cell of fermented liquid 1～2% for adding weight, opens and stirs, and direct heating to 80～95 ℃ pump into sheet frame after fully stirring and filter.
Being preferably described fermented liquid coarse filtration, to add weight be the super-cell of fermented liquid 1.5%, direct heating to 90 ℃.
Described enzyme is treated to the fermented liquid after the coarse filtration is pumped into enzymatic vessel; With sig water fermented liquid is transferred to 7.5～8.0; Be cooled to after 50～60 ℃ the Sumizyme MP effect 30 minutes that weight is fermented liquid 0.2～0.4% that adds; Transfer PH to 6.0～7.0 after being cooled to 30～40 ℃ more again, adding weight is the Sodium hexametaphosphate 99 and N,O-Diacetylmuramidase effect 1 hour of fermented liquid 0.1～0.2%, 90～95 ℃ of enzymes processing of going out of high temperature behind the enzymolysis.
Being preferably described enzyme is treated to the fermented liquid after the coarse filtration is pumped into enzymatic vessel; With sig water fermented liquid is transferred to 8.0, add Sumizyme MP after being cooled to 60 ℃ to do, transfer PH to 7.0 again after being cooled to 40 ℃ again; Add Sodium hexametaphosphate 99 and N,O-Diacetylmuramidase, the high temperature enzyme that goes out is handled behind the enzymolysis.
Described essence is filtered into the fermented liquid that enzyme was handled and transfers PH to 4～6, and the ultra-filtration membrane that pumps into 5000D is held back 70～85% of simmer down to original fermented solution weight, removes small molecular weight impurity and pigment.
Be preferably the described smart fermented liquid that enzyme was handled that filters and transfer PH to 4.
Described organic solvent deposit, solid-liquid separation are that the liquid concentrator that ultra-filtration membrane is held back pumps in the bleacher, slowly adds 1～2 times of 95% above ethanol, after fully stirring, pumps into sheet frame and carries out press dewatering; The organic solvent deposit time is 30～40 minutes, and solid-liquid separation pressure is 0.1～0.4Mpa.
Described drying, pulverize and to do, adopt recirculating air or fluidized drying, 70～90 ℃ down oven dry to obtain moisture in 2～4 hours be the gelling gum 12% below, fully pulverize mixing through pulverizing mill again and obtain transparent type high acyl gellan gum finished product.
The extraction process of transparent type high acyl gellan gum of the present invention, technology are simply novel, and cost is low, and yield is high, and the product transparency of being produced is high, and purity is high, and solvability is good.
Embodiment:
Embodiment 1
Fermented liquid of the present invention be moving slightly Sphingol single-cell under aerobic condition, be carbon source with glucose or sucrose, be nitrogenous source with yeast extract paste and/or peptone, other be an inorganic salts, fermentation and must fermented liquid.
The novel technology for extracting of a kind of transparent type high acyl gellan gum of the present invention comprises the steps:
(1) fermented liquid coarse filtration
With the 100Kg fermented liquid is example, at first with the 100Kg fermented liquid direct heating to 90 of fermentation ends ℃, in fermented liquid, adds the zeyssatite of 5Kg; The zeyssatite that in the precoating jar, adds 10Kg is warming up to 90～95 ℃, and precoating is pumped into sheet frame night, treat filtering after, the fermented liquid after the heating is pumped into Plate Filtration.
(2) fermentation broth enzyme is handled
Fermented liquid after the coarse filtration pumps into enzymatic vessel; With sig water fermented liquid is transferred to 8.0, be cooled to 60 ℃, the Sumizyme MP effect that adds 0.3Kg is after 30 minutes; Add the 0.1Kg Sodium hexametaphosphate 99 and transfer PH to 7.0 again; Be cooled to 40 ℃, add after the 0.1Kg N,O-Diacetylmuramidase fully acts on 1 hour, and then be warming up to 90 ℃ and carry out the high temperature enzyme that goes out and handle.
(3) ultrafiltration membrance filter
Fermented liquid behind the enzymolysis is transferred pH value 4.0, be cooled to 50 ℃, the ultra-filtration membrane that pumps into 5000D is held back concentrated, removes small molecular weight impurity and pigment, and the gelling gum liquid concentrator weight after holding back is 80Kg.
(4) organic solvent deposit, solid-liquid separation
The liquid concentrator that ultra-filtration membrane is held back pumps in the bleacher, slowly adds 160Kg content and be 95% ethanolic soln, fully stir 30 minutes after, pump into sheet frame and carry out press dewatering.The described organic solvent deposit time, solid-liquid separation pressure was 0.3Mpa in order to be 30 minutes.
(5) dry, pulverizing
Through the gelling gum behind the press dewatering, adopt recirculating air or fluidized drying, under 85 ℃, drying and obtaining moisture in 3 hours is the gelling gum below 12%; Fully pulverize through pulverizing mill again and mix that to obtain fineness be that 80 order transmitances reach the transparent type high acyl gellan gum finished product more than 95%.
Embodiment 2
Fermented liquid of the present invention be moving slightly Sphingol single-cell under aerobic condition, be carbon source with glucose or sucrose, be nitrogenous source with yeast extract paste and/or peptone, other be an inorganic salts, fermentation and must fermented liquid.
The novel technology for extracting of a kind of transparent type high acyl gellan gum of the present invention comprises the steps:
(1) fermented liquid coarse filtration
With the 100Kg fermented liquid is example, at first with the 100Kg fermented liquid direct heating to 90 of fermentation ends ℃, in fermented liquid, adds the zeyssatite of 5Kg; The zeyssatite that in the precoating jar, adds 5Kg is warming up to 90～95 ℃, and precoating is pumped into sheet frame night, treat filtering after, the fermented liquid after the heating is pumped into Plate Filtration.
(2) fermentation broth enzyme is handled
Fermented liquid after the coarse filtration pumps into enzymatic vessel; With sig water fermented liquid is transferred to 7.5, be cooled to 50 ℃, the Sumizyme MP effect that adds 0.2Kg is after 30 minutes; Add the 0.15Kg Sodium hexametaphosphate 99 and transfer PH to 7.0 again; Be cooled to 30 ℃, add after the 0.15Kg N,O-Diacetylmuramidase fully acts on 1 hour, and then be warming up to 95 ℃ and carry out the high temperature enzyme that goes out and handle.
(3) ultrafiltration membrance filter
Fermented liquid behind the enzymolysis is transferred pH value 4.6, be cooled to 45 ℃, the ultra-filtration membrane that pumps into 5000D is held back concentrated, removes small molecular weight impurity and pigment, and the gelling gum liquid concentrator weight after holding back is 80Kg.
(4) organic solvent deposit, solid-liquid separation
The liquid concentrator that ultra-filtration membrane is held back pumps in the bleacher, slowly adds 150Kg content and be 95% ethanolic soln, fully stir 35 minutes after, pump into sheet frame and carry out press dewatering.The described organic solvent deposit time, solid-liquid separation pressure was 0.3Mpa in order to be 35 minutes.
(5) dry, pulverizing
Through the gelling gum behind the press dewatering, adopt recirculating air or fluidized drying, under 80 ℃, drying and obtaining moisture in 3 hours is the gelling gum below 12%; Fully pulverize through pulverizing mill again and mix that to obtain fineness be that 80 order transmitances reach the transparent type high acyl gellan gum finished product more than 95%.
Embodiment 3
Fermented liquid of the present invention be moving slightly Sphingol single-cell under aerobic condition, be carbon source with glucose or sucrose, be nitrogenous source with yeast extract paste and/or peptone, other be an inorganic salts, fermentation and must fermented liquid.
The novel technology for extracting of a kind of transparent type high acyl gellan gum of the present invention comprises the steps:
(1) fermented liquid coarse filtration
With the 100Kg fermented liquid is example, at first with the 100Kg fermented liquid direct heating to 90 of fermentation ends ℃, in fermented liquid, adds the zeyssatite of 5Kg; The zeyssatite that in the precoating jar, adds 15Kg is warming up to 90～95 ℃, and precoating is pumped into sheet frame night, treat filtering after, the fermented liquid after the heating is pumped into Plate Filtration.
(2) fermentation broth enzyme is handled
Fermented liquid after the coarse filtration pumps into enzymatic vessel; With sig water fermented liquid is transferred to 7.8, be cooled to 55 ℃, the Sumizyme MP effect that adds 0.3Kg is after 30 minutes; Add the 0.2Kg Sodium hexametaphosphate 99 and transfer PH to 7.0 again; Be cooled to 35 ℃, add after the 0.2Kg N,O-Diacetylmuramidase fully acts on 1 hour, and then be warming up to 95 ℃ and carry out the high temperature enzyme that goes out and handle.
(3) ultrafiltration membrance filter
Fermented liquid behind the enzymolysis is transferred pH value 5.0, be cooled to 55 ℃, the ultra-filtration membrane that pumps into 5000D is held back concentrated, removes small molecular weight impurity and pigment, and the gelling gum liquid concentrator weight after holding back is 80Kg.
(4) organic solvent deposit, solid-liquid separation
The liquid concentrator that ultra-filtration membrane is held back pumps in the bleacher, slowly adds 100Kg content and be 95% ethanolic soln, fully stir 40 minutes after, pump into sheet frame and carry out press dewatering.The described organic solvent deposit time, solid-liquid separation pressure was 0.3Mpa in order to be 40 minutes.
(5) dry, pulverizing
Through the gelling gum behind the press dewatering, adopt recirculating air or fluidized drying, under 88 ℃, drying and obtaining moisture in 3 hours is the gelling gum below 12%; Fully pulverize through pulverizing mill again and mix that to obtain fineness be that 80 order transmitances reach the transparent type high acyl gellan gum finished product more than 95%.
Claims (9)
1. the extraction process of a transparent type high acyl gellan gum; It is characterized in that: will be carbon source with glucose or sucrose at first, be that nitrogen source fermentation gets the fermented liquid coarse filtration with yeast extract paste and/or peptone, 40～60 ℃ of temperature; Under the condition of pH value 6～8; After adding proteolytic enzyme and N,O-Diacetylmuramidase fully act on, pump into ultra-filtration membrane and carry out the essence filtration, make transparent type high acyl gellan gum product through organic solvent deposit, solid-liquid separation, drying, pulverizing then.
2. the extraction process of a kind of transparent type high acyl gellan gum according to claim 1; It is characterized in that: described fermented liquid coarse filtration is the super-cell of fermented liquid 1～2% for adding weight; Open to stir, direct heating to 80～95 ℃ pump into sheet frame after fully stirring and filter.
3. the extraction process of a kind of transparent type high acyl gellan gum according to claim 2 is characterized in that: it is the super-cell of fermented liquid 1.5% that described fermented liquid coarse filtration adds weight, direct heating to 90 ℃.
4. the extraction process of a kind of transparent type high acyl gellan gum according to claim 1; It is characterized in that: described enzyme is treated to the fermented liquid after the coarse filtration is pumped into enzymatic vessel; With sig water fermented liquid is transferred to 7.5～8.0; Be cooled to after 50～60 ℃ the Sumizyme MP effect 30 minutes that weight is fermented liquid 0.2～0.4% that adds; Transfer PH to 6.0-7.0 after being cooled to 30～40 ℃ more again, adding weight is the Sodium hexametaphosphate 99 and N,O-Diacetylmuramidase effect 1 hour of fermented liquid 0.1～0.2%, 90～95 ℃ of enzymes processing of going out of high temperature behind the enzymolysis.
5. the extraction process of a kind of transparent type high acyl gellan gum according to claim 4; It is characterized in that: described enzyme is treated to the fermented liquid after the coarse filtration is pumped into enzymatic vessel; With sig water fermented liquid is transferred to 8.0, add Sumizyme MP after being cooled to 60 ℃ to do, transfer PH to 7.0 again after being cooled to 40 ℃ again; Add Sodium hexametaphosphate 99 and N,O-Diacetylmuramidase, the high temperature enzyme that goes out is handled behind the enzymolysis.
6. the extraction process of a kind of transparent type high acyl gellan gum according to claim 1; It is characterized in that: described essence is filtered into the fermented liquid that enzyme was handled and transfers PH to 4～6; The ultra-filtration membrane that pumps into 5000D is held back 70～85% of simmer down to original fermented solution weight, removes small molecular weight impurity and pigment.
7. the extraction process of a kind of transparent type high acyl gellan gum according to claim 6 is characterized in that: the described smart fermented liquid that enzyme was handled that filters is transferred PH to 4.
8. the extraction process of a kind of transparent type high acyl gellan gum according to claim 1; It is characterized in that: described organic solvent deposit, solid-liquid separation do; The liquid concentrator that ultra-filtration membrane is held back pumps in the bleacher; Slowly add 1～2 times of 95% above ethanol, fully stir 30～40 minutes after, pump into sheet frame and carry out press dewatering; The organic solvent deposit time is 30～40 minutes, and solid-liquid separation pressure is 0.1～0.4Mpa.
9. the extraction process of a kind of transparent type high acyl gellan gum according to claim 1; It is characterized in that: described drying, pulverizing do; Adopt recirculating air or fluidized drying; Under 70～90 ℃, drying and obtaining moisture in 2～4 hours is the gelling gum below 12%, fully pulverizes to mix through pulverizing mill again to obtain transparent type high acyl gellan gum finished product.
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| CN201010222411.7A CN102311508B (en) | 2010-07-09 | 2010-07-09 | Process for extracting transparent high-acyl gellan gum |
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| CN201010222411.7A CN102311508B (en) | 2010-07-09 | 2010-07-09 | Process for extracting transparent high-acyl gellan gum |
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| CN102311508B CN102311508B (en) | 2014-05-14 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103509845A (en) * | 2013-08-09 | 2014-01-15 | 新疆阜丰生物科技有限公司 | High aryl gellan gum extracting method |
| CN108690143A (en) * | 2017-04-07 | 2018-10-23 | 帝斯曼知识产权资产管理有限公司 | A kind of production method of high transparency low-acyl gellan gum |
| CN111936523A (en) * | 2018-04-04 | 2020-11-13 | 科莱恩国际有限公司 | Methods for purifying complex biological compositions |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6602996B1 (en) * | 1998-06-10 | 2003-08-05 | Cp Kelco U.S., Inc. | Modified gellan gum composition process for preparation of same and use thereof |
| CN101460065A (en) * | 2006-12-15 | 2009-06-17 | Cp凯尔科美国股份有限公司 | Ultra high performance clarified gellan gums |
| CN101665778A (en) * | 2009-09-25 | 2010-03-10 | 浙江大学 | Uranidin generation deficiency sphingolipid sphingomonas paucimobilis and application thereof in gellan gum production |
-
2010
- 2010-07-09 CN CN201010222411.7A patent/CN102311508B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6602996B1 (en) * | 1998-06-10 | 2003-08-05 | Cp Kelco U.S., Inc. | Modified gellan gum composition process for preparation of same and use thereof |
| CN101460065A (en) * | 2006-12-15 | 2009-06-17 | Cp凯尔科美国股份有限公司 | Ultra high performance clarified gellan gums |
| CN101665778A (en) * | 2009-09-25 | 2010-03-10 | 浙江大学 | Uranidin generation deficiency sphingolipid sphingomonas paucimobilis and application thereof in gellan gum production |
Non-Patent Citations (2)
| Title |
|---|
| 朱圣东等: "超滤-醇析提取黄原胶工艺条件", 《过程工程学报》, vol. 4, no. 6, 30 December 2004 (2004-12-30), pages 525 - 530 * |
| 王桂兰和凌沛学: "细菌胞外多糖提取分离技术研究进展", 《食品与药品》, vol. 12, no. 5, 10 May 2010 (2010-05-10), pages 217 - 219 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103509845A (en) * | 2013-08-09 | 2014-01-15 | 新疆阜丰生物科技有限公司 | High aryl gellan gum extracting method |
| CN108690143A (en) * | 2017-04-07 | 2018-10-23 | 帝斯曼知识产权资产管理有限公司 | A kind of production method of high transparency low-acyl gellan gum |
| US10556970B2 (en) | 2017-04-07 | 2020-02-11 | Dsm Ip Assets B.V. | Method for preparing high transparent low acyl gellan gum |
| CN111936523A (en) * | 2018-04-04 | 2020-11-13 | 科莱恩国际有限公司 | Methods for purifying complex biological compositions |
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