CN106434443B - A kind of production technology of Sodium Hyaluronate - Google Patents
A kind of production technology of Sodium Hyaluronate Download PDFInfo
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- CN106434443B CN106434443B CN201610844974.7A CN201610844974A CN106434443B CN 106434443 B CN106434443 B CN 106434443B CN 201610844974 A CN201610844974 A CN 201610844974A CN 106434443 B CN106434443 B CN 106434443B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/46—Streptococcus ; Enterococcus; Lactococcus
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
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Abstract
The invention discloses a kind of production technology of Sodium Hyaluronate.The present invention filters out the high strain of hyaluronic acid productivity, with short production cycle with reference to improved fermentation technique, simple production process, supplementary material is common to be easy to get, and production is easily controllable, and impurity content is lower, it is adapted to industrialization large-scale production to require, high income, and greatly reduce production cost.
Description
Technical field
The present invention relates to hyaluronic acid production technical field, more particularly to a kind of production technology of Sodium Hyaluronate.
Background technology
The technique fermentation period length that Production by Microorganism Fermentation hyaluronic acid uses, zymotechnique cannot be according to strain properties
Carry out, cause bacterial activity to be excited, hyaluronic acid productivity is low, poor product quality.Contain thalline, protein in zymotic fluid
And the material such as nucleic acid, extraction hyaluronic acid not only requires high recovery rate and its molecule that depolymerization does not occur, and requires high
Product purity, and from zymotic fluid extract hyaluronic acid technical process can directly affect hyaluronic acid yield and quality and
Production cost.
New fermentation strain is developed, meanwhile, improve zymotechnique according to strain properties and extraction process is transparent to improve
The quality and yield of matter acid sodium, are the technical problem to be solved in the invention.
The content of the invention
The purpose of the present invention provides a kind of production technology of Sodium Hyaluronate aiming at above-mentioned defect.This hair
Bright to filter out the high strain of hyaluronic acid productivity, with short production cycle with reference to improved fermentation technique, simple production process is former auxiliary
Expect it is common be easy to get, production is easily controllable, and impurity content is lower, is adapted to industrialization large-scale production to require, high income, and significantly
Reduce production cost.
A kind of production Technology scheme of Sodium Hyaluronate of the present invention is to comprise the following steps：
（1）Malian drainage（Streptococcus equi subsp. zooepidemicus）DCS005 shakes
Bottle seed liquor culture；
（2）Strain expands culture；
（3）Fermentation, is first passed through sterile oxygen-containing air fermentation, then is passed through nitrogen and continues to ferment；
（4）Zymotic fluid is diluted with water to 6-8 times of fermentating liquid volume, diatomite and activated carbon are added into dilution,
Plate compression after 40 DEG C of insulated and stirreds, collects filtrate；Ultrafiltration is carried out to filtrate, removes metal ion and the colored substance of small molecule
Matter；
（5）Solution after ultrafiltration is added to the sodium chloride of 0.6mol/L, ethanol is added until completely dissolved, is precipitated to end
Point Alcohol degree is 60%-65%, is sufficiently stirred, staticly settles, and takes out sediment Sodium Hyaluronate；Supernatant is filtered, so
Stream plus the absolute ethyl alcohol of 0.3-2 times of volume afterwards, collected after centrifugation precipitation；
(6) washing dehydration is dried：It will be dried in vacuo after Sodium Hyaluronate precipitates washed with EtOH, dehydration, up to thoroughly
Bright matter acid sodium finished product.
Malian drainage used in the present invention（Streptococcus equi subsp. zooepidemicus）
DCS005, the Malian drainage is in China Committee for Culture Collection of Microorganisms's common micro-organisms center guarantor at present
Hide, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, postcode：100101, its deposit number is：CGMCC No.12782,
The Latin title of strain isStreptococcus equi subsp. zooepidemicus, join the microorganism of evidence（Strain）：
DCS005, preservation date are on July 14th, 2016.
Step（1）、（2）、（3）In nutrient solution include starch 1%, sucrose 0.5%, beef extract by weight percentage
0.5%th, dusty yeast 0.5%, dipotassium hydrogen phosphate 0.02%, sodium hydroxide 0.05%, surplus are water, pH7.0-7.3.
Step（1）In, condition of culture is：When 38.5 DEG C of culture 12-16 are small.
Step（2）In, condition of culture is 38.5 DEG C of temperature, pH7.0-7.3, when incubation time is 12-16 small, tank pressure
0.05MPa。
Step（3）Specifically, be passed through 37-40 DEG C of fermentation 18-32 of sterile oxygen-containing air it is small when after, be passed through 39-45 DEG C of nitrogen
Continue to ferment, be passed through the nitrogen stage add at the same time concentration be 800g/L glucose 300mL, zymotic fluid pH falls per minute
Terminate to ferment during less than 0.01, detect fermentation broth viscosity, zymotic fluid is pressed into settling tank, wherein, fermentation tank tank pressure 0.05MP.
Diatomite addition is the 2% of fermentating liquid volume, and activated carbon addition is the 2% of fermentating liquid volume.
Ultrafiltration retaining molecular weight used in ultrafiltration is 30,000-50,000, and ultrafiltration is carried out in below 0.5MPa.
Beneficial effects of the present invention are：
The present invention filters out the high strain of hyaluronic acid productivity, with short production cycle with reference to improved fermentation technique, produces work
Skill is simple, and supplementary material is common to be easy to get, and production is easily controllable, and impurity content is lower, is adapted to industrialization large-scale production to require, yield
Height, and greatly reduce production cost.Can the inexpensive Sodium Hyaluronate that high-purity is made, hyaluronic acid and its purity salt height
Up to 99.6%, glucuronic acid purity is more than 45%, and loss of molecular weight rate is less than 11%, and protein content is less than 0.04%.
Embodiment：
For a better understanding of the present invention, below with instantiation come the technical solution that the present invention will be described in detail, but this
Invention is not limited thereto.
Embodiment 1
Malian drainage used in the present invention（Streptococcus equi subsp. zooepidemicus）
DCS005, the Malian drainage is in China Committee for Culture Collection of Microorganisms's common micro-organisms center guarantor at present
Hide, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, postcode：100101, its deposit number is：CGMCC No.12782,
The Latin title of strain isStreptococcus equi subsp. zooepidemicus, join the microorganism of evidence（Strain）：
DCS005, preservation date are on July 14th, 2016.
A kind of production technology of Sodium Hyaluronate, comprises the following steps：
（1）Malian drainage（Streptococcus equi subsp. zooepidemicus）DCS005 shakes
Bottle seed liquor culture, condition of culture are：When 38.5 DEG C of culture 12-16 are small；
（2）Strain expands culture, and condition of culture is 38.5 DEG C of temperature, pH7.0-7.3, when incubation time is 12-16 small, tank
Press 0.05MPa；
（3）Fermentation, be passed through 37-40 DEG C of fermentation 18-32 of sterile oxygen-containing air it is small when after, be passed through 39-45 DEG C of nitrogen after supervention
Ferment, be passed through the nitrogen stage adds the glucose 300mL that concentration is 800g/L at the same time, and zymotic fluid pH falls per minute are less than
Terminate to ferment when 0.01, detect fermentation broth viscosity, zymotic fluid is pressed into settling tank, wherein, fermentation tank tank pressure 0.05MP；
（4）Zymotic fluid is diluted with water to 6-8 times of fermentating liquid volume, the silicon of fermentating liquid volume 2% is added into dilution
The activated carbon of diatomaceous earth and fermentating liquid volume 2%, plate compression after 40 DEG C of insulated and stirreds, collects filtrate；Use and cut in below 0.5MPa
Stay the ultrafiltration membrane that molecular weight is 30,000-50,000 to carry out ultrafiltration to filtrate, remove metal ion and the coloring matter of small molecule；
（5）Solution after ultrafiltration is added to the sodium chloride of 0.6mol/L, ethanol is added until completely dissolved, is precipitated to end
Point Alcohol degree is 60%-65%, is sufficiently stirred, staticly settles, and takes out sediment Sodium Hyaluronate；Supernatant is filtered, so
Stream plus the absolute ethyl alcohol of 0.3-2 times of volume afterwards, collected after centrifugation precipitation；
(6) washing dehydration is dried：It will be dried in vacuo after Sodium Hyaluronate precipitates washed with EtOH, dehydration, up to thoroughly
Bright matter acid sodium finished product.
Step（1）、（2）、（3）In nutrient solution include starch 1%, sucrose 0.5%, beef extract by weight percentage
0.5%th, dusty yeast 0.5%, dipotassium hydrogen phosphate 0.02%, sodium hydroxide 0.05%, surplus are water, pH7.0-7.3.
The Sodium Hyaluronate of the obtained high-purity of energy low cost of the invention, hyaluronic acid and its purity salt are up to 99.6%, grape
Uronic acid purity is more than 45%, and loss of molecular weight rate is less than 11%, and protein content is less than 0.04%.
Claims (1)
1. a kind of production technology of Sodium Hyaluronate, it is characterised in that comprise the following steps：
（1）Malian drainage（Streptococcus equi subsp. zooepidemicus）DCS005 shaking flask kinds
Sub- liquid culture, condition of culture are：When 38.5 DEG C of culture 12-16 are small；
（2）Strain expands culture, and condition of culture is 38.5 DEG C of temperature, pH7.0-7.3, when incubation time is 12-16 small, tank pressure
0.05MPa；
（3）Fermentation, be passed through 37-40 DEG C of fermentation 18-32 of sterile oxygen-containing air it is small when after, be passed through 39-45 DEG C of nitrogen and continue to ferment, lead to
Enter the nitrogen stage add at the same time concentration be 800g/L glucose 300mL, zymotic fluid pH falls per minute be less than 0.01 when
Terminate fermentation, detect fermentation broth viscosity, zymotic fluid is pressed into settling tank, wherein, fermentation tank tank pressure 0.05MP；
（4）Zymotic fluid is diluted with water to 6-8 times of fermentating liquid volume, addition diatomite and activated carbon into dilution, 40 DEG C
Plate compression after insulated and stirred, collects filtrate；Ultrafiltration is carried out to filtrate；Diatomite addition is the 2% of fermentating liquid volume, active
Charcoal addition is the 2% of fermentating liquid volume；Ultrafiltration retaining molecular weight used in ultrafiltration is 30,000-50,000, and ultrafiltration is in 0.5MPa
Carry out below；
（5）Solution after ultrafiltration is added to the sodium chloride of 0.6mol/L, adds ethanol until completely dissolved, precipitates wine to terminal
Precision number is 60%-65%, is sufficiently stirred, staticly settles, and takes out sediment Sodium Hyaluronate；Supernatant is filtered, Ran Houliu
Add the absolute ethyl alcohol of 0.3-2 times of volume, collected after centrifugation precipitation；
（ 6）Washing dehydration is dried：It will be dried in vacuo after Sodium Hyaluronate precipitates washed with EtOH, dehydration, up to hyalomitome
Sour sodium finished product；
The Malian drainage（Streptococcus equi subsp. zooepidemicus）DCS005, its
In China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation, its deposit number is：CGMCC No.12782,
Preservation date is on July 14th, 2016；
Step（1）、（2）、（3）In nutrient solution include starch 1%, sucrose 0.5%, beef extract 0.5%, ferment by weight percentage
Female powder 0.5%, dipotassium hydrogen phosphate 0.02%, sodium hydroxide 0.05%, surplus are water, pH7.0-7.3.
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| CN201610844974.7A CN106434443B (en) | 2016-09-23 | 2016-09-23 | A kind of production technology of Sodium Hyaluronate |
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| CN106434443B true CN106434443B (en) | 2018-05-15 |
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| CN109913518A (en) * | 2018-08-17 | 2019-06-21 | 上海昊海生物科技股份有限公司 | A kind of pipeline preparation method of Sodium Hyaluronate |
| CN109536550A (en) * | 2018-12-06 | 2019-03-29 | 上海景峰制药有限公司 | A kind of Sodium Hyaluronate and preparation method thereof |
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| CN1102435A (en) * | 1994-08-17 | 1995-05-10 | 桂平县生物技术开发公司 | Manufacturing method of transparent sodium salt with microbiogical ferment |
| CN101643760A (en) * | 2009-09-04 | 2010-02-10 | 四川理工学院 | Method for increasing the yield of hyaluronic acid |
| CN103173507A (en) * | 2011-12-23 | 2013-06-26 | 宁波林叶生物科技有限公司 | Production technology for fermentatively producing sodium hyaluronate by utilizing bacterium |
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Effective date of registration: 20200826 Address after: 257000 No.75 Huaihe Road, Dongying Economic Development Zone, Shandong Province Patentee after: DONGYING FOSTER BIOLOGICAL ENGINEERING Co.,Ltd. Address before: 257000, Shandong, Dongying, Kenli Town, West Tuo (Yong Xin Road, No. 98) Co-patentee before: DONGYING FOSTER BIOLOGICAL ENGINEERING Co.,Ltd. Patentee before: CHINA EASTAR HOLDINGS GROUP Co.,Ltd. |