CN102302560B - Composition of lucid ganoderma extract and tartary buckwheat extract, as well as application thereof - Google Patents
Composition of lucid ganoderma extract and tartary buckwheat extract, as well as application thereof Download PDFInfo
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Abstract
The invention discloses a composition of lucid ganoderma extract and tartary buckwheat extract, as well as application thereof. The composition is prepared by extracting lucid ganoderma and tartary buckwheat which have the same mass, wherein the composition contains the following active components: 3.0 to 8.0 percent of lucid ganoderma total triterpenoid, 15.0 to 20.0 percent of lucid ganoderma polysaccharide, and 18.0 to 25.0 percent of tartary buckwheat total triterpenoid. The composition has strong anti-oxidation activity, and can effectively eliminate hydroxy free radical, and the effect of the composition is stronger than that of single lucid ganoderma extract or tartary buckwheat extract. The composition can be used for preparing a medicament or health-care product for preventing and treating diseases related to oxidative damages.
Description
Technical field
The present invention relates to a kind of compositions and application thereof of extract, particularly relate to compositions and the application thereof of a kind of Ganoderma extract and tartary buckwheat extract.
Background technology
Hydroxy radical is a kind of free radical with very strong oxidative damage ability, can make more intracellular biomacromolecule generation oxidations and causes membrane damage, enzyme deactivation, the normal biochemical stopping of reaction even dna mutation.Human body can produce more hydroxy radical under certain pathological conditions, various diseases is proved to be oxidative damage relevant [Schmidt N, Ferger B.Brain Res, 2004,1012 (1-2): the 101-107 due to the hydroxy radical that produces with body; Wang S, Leonard S S, Ye J, et al.Am J Physiol Cell Physiol, 2000,279 (3): C868-C875; Chen C L, Chi C W, Liu T Y.J Toxicol Environ Health A, 2002,65 (3-4): 327-336].The research hydroxy radical scavenger is for preventing or/and the treatment various diseases is significant from the antioxidation angle.
Ganoderma is a kind of mushroom Chinese medicine, makes medicinal and edible existing thousands of years history in China, now throughout the country extensively cultivation.Ganoderma has invigorating QI and tranquilization, and the effect of relieving cough and asthma is usually used in malaise, insomnia cardiopalmus, and cough and asthma due to lung deficiency, asthenia is losed heart, and the clinical diseases such as anorexia are also having a lot of application aspect defying age, antitumor, the blood fat reducing.The chemical composition of Ganoderma is mainly polysaccharide and triterpenes.Ganoderan is a class glucose by β-(1 → 3) and macromolecule non-starch polysaccharides(nsp) that β-(1 → 6) glycosidic bond is polymerized; The main pharmacological of ganoderan has: regulate immunity, antitumor, defying age, blood sugar lowering.The triterpenes components of having found in the Ganoderma has over one hundred kind, mostly be the lanostane derivant, different Ganoderma acids, Ganoderma lucidum (Leyss. Ex Fr.) Karst. acids, Ganoderma alcohols, the Ganolactone classes etc. of being divided into according to contained functional group and side chain, representative composition such as ganoderic acid B, lucidenic acid A, ganoderol D, 7 beta-hydroxies-3,11,15-, three oxygen lanoline sterones-8-alkene-24 (20)-lactone; The main pharmacological of Ganoderma lucidum triterpenes components has: protect the liver, antitumor, blood sugar lowering, anti-HIV-1, anti-blood pressure, suppress histamine release, antioxidation.
Radix Et Rhizoma Fagopyri Tatarici is a kind of medicine-food two-purpose resource, has a large amount of cultivations and quilt extensively edible and medicinal in autonomous prefecture of the Liangshan of Sichuan Province Yi nationality, distributed over Yunnan, Sichuan and Guizhou.Radix Et Rhizoma Fagopyri Tatarici has the spleen invigorating row and stagnates, regulating QI to relieve pain, and the effect of removing toxic substances and promoting subsidence of swelling is used for epigastric distending pain traditionally, dyspepsia, lumbago and skelalgia, traumatic injury, the clinical diseases such as carbuncle sore tumefacting virus also are applied to the diseases such as diabetes, hyperlipidemia, tumor in recent years.The effective ingredient of Radix Et Rhizoma Fagopyri Tatarici is mainly flavonoid, representative composition such as rutin, Quercetin, nimbecetin, Quercetin-3-rutinoside-7-galactoside, the two rhamnosides of Quercetin-3-, Kaempferol-O-rutinoside.The main pharmacological of Radix Et Rhizoma Fagopyri Tatarici flavone constituents is: blood sugar lowering, blood fat reducing, atherosclerosis, antithrombotic formation, antioxidation, antitumor, uvioresistant effect.
At present existing Ganoderma extract and tartary buckwheat extract, but take separately Ganoderma extract or tartary buckwheat extract, owing to there not being cooperative effect, its antioxygenic property is starkly lower than the compositions of Ganoderma extract and tartary buckwheat extract.There is no at present the compositions of Ganoderma extract and tartary buckwheat extract and the relevant report of preparation thereof.
Summary of the invention
The object of the present invention is to provide and a kind ofly can effectively remove hydroxy radical, the compositions that contains Ganoderma extract and tartary buckwheat extract that oxidation resistance is strong.
Another object of the present invention is to provide said composition in the medicine for preparing prevention or the treatment disease relevant with oxidative damage or the application in the health product.
For achieving the above object, the invention provides the compositions of a kind of Ganoderma extract and tartary buckwheat extract, the contained Ganoderma extract of said composition and tartary buckwheat extract are made by Ganoderma and the Radix Et Rhizoma Fagopyri Tatarici extraction of equal in quality,
Wherein Ganoderma extract makes by the following method:
(1) first Ganoderma is pulverized, then with 70.0%～95.0% ethanol (being that the 1g Ganoderma the adds 5～10ml ethanol) reflux, extract, of 5～10 times of Ganoderma quality amount volumes 3 times, each 1h, or extract with 70.0%～95.0% ethanol (being that the 1g Ganoderma the adds 15～25ml ethanol) percolation of 15～25 times of Ganoderma quality amount volumes, behind the extracting solution Recycled ethanol with reflux, extract, or percolation extraction, be splined on siliceous earth column, used diatomaceous quality is 1～1.5 times of Ganoderma quality, then use the eluent ethyl acetate of 4～6 times of column volumes, collect eluent, reclaim ethyl acetate, get extract A;
(2) get Ganoderma medicinal residues behind above-mentioned 70.0%～95.0% ethanol extraction, with water boiling and extraction 2 times, 10～15 times of water (being that 1g Ganoderma medicinal residues add 10～15ml water) of measuring volumes with Ganoderma medicinal residues quality extract 1h at every turn, it is 1.10～1.20 that extracting solution is concentrated into relative density, then adding 95.0% ethanol is 60%～70% to containing the alcohol amount, after precipitating fully, filters, get filtration cakes torrefaction, get extract B;
(3) united extraction thing A and extract B, and mix homogeneously namely get Ganoderma extract;
Wherein tartary buckwheat extract makes by the following method:
Radix Et Rhizoma Fagopyri Tatarici is pulverized, then with 70.0%～95.0% ethanol (being that the 1g Radix Et Rhizoma Fagopyri Tatarici the adds 5～10ml ethanol) reflux, extract, of 5～10 times of Radix Et Rhizoma Fagopyri Tatarici quality amount volumes 3 times, each 1h, or extract with 70.0%～95.0% ethanol (being that the 1g Radix Et Rhizoma Fagopyri Tatarici the adds 15～25ml ethanol) percolation of 15～25 times of Radix Et Rhizoma Fagopyri Tatarici quality amount volumes, behind the extracting solution Recycled ethanol with reflux, extract, or percolation extraction, be splined on macroporous resin column, used resin quality is 1.5～2.5 times of Radix Et Rhizoma Fagopyri Tatarici quality, follow the washing decontamination with 3～5 times of column volumes, use again 50.0%～80.0% ethanol elution of 5～10 times of column volumes, collect ethanol elution, Recycled ethanol, concentrated, drying namely gets tartary buckwheat extract.
The present composition adds the excipient of pharmaceutically accepting and can be made into capsule, tablet, pill, granule, oral liquid or medicated wine.
The present composition can be applicable to prepare medicine or the health product of prevention or the treatment disease relevant with oxidative damage.
The percentage by weight of the contained main active substances of the present composition is as follows: Ganoderma total triterpenes 3.0%～8.0%, ganoderan 15.0%～20.0%, Radix Et Rhizoma Fagopyri Tatarici total flavonoids 18.0%～25.0%.
The content of above-mentioned main effective ingredient is measured as follows:
(1) Ganoderma total triterpenes
The preparation of reference substance solution: get the ursolic acid reference substance an amount of, accurately weighed, add methanol and make the solution that every 1ml contains 0.1mg, and get final product.
The preparation of standard curve: precision measures reference substance solution 0.2ml, 0.4ml, 0.6ml, 0.8ml, 1.0ml, 1.2ml, puts respectively in the 10ml tool plug test tube water bath method, respectively add 5% vanillin-glacial acetic acid solution 0.5ml and perchloric acid 1.0ml, put in 60 ℃ of water-baths and be incubated 15min, take out, the frozen water cooling, ice acetic acid shakes up to 10ml, take corresponding reagent as blank, measure absorbance in the 548nm place, take absorbance as vertical coordinate, concentration is abscissa, the drawing standard curve.
The preparation of need testing solution: get approximately 1g of extract, accurately weighed, put in the apparatus,Soxhlet's, add ethyl acetate 90ml, reflux shifts extracting solution to the 100ml measuring bottle to extracting liquid colourless, adds ethyl acetate to scale, shakes up; Precision measures 10ml, puts in the 100ml measuring bottle, adds ethyl acetate to scale, shakes up, and get final product.
Algoscopy: precision measures need testing solution 2ml, and the method under the preparation of sighting target directrix curve from " water bath method ", is measured absorbance in accordance with the law, presses the content that standard curve calculates Ganoderma total triterpenes.
(2) ganoderan
The preparation of reference substance solution: get 105 ℃ of anhydrous glucose reference substances that are dried to constant weight an amount of, accurately weighed, add water and make the solution that every 1ml contains 0.1mg, and get final product.
The preparation of standard curve: precision measures reference substance solution 0.2ml, 0.4ml, 0.6ml, 0.8ml, 1.0ml, 1.2ml, put respectively in the 10ml tool plug test tube, add water to 2.0ml, (precision takes by weighing anthrone 0.1g to accurate adding sulphuric acid anthrone solution, add 80% sulfuric acid solution 100ml and make solution, shake up) 6ml, shake up, put and heat 15min in the water-bath, take out, put into ice-water bath and cool off 15min, take corresponding reagent as blank, measure absorbance in the 625nm place, take absorbance as vertical coordinate, concentration is abscissa, the drawing standard curve.
The preparation of need testing solution: get approximately 1g of extract, accurately weighed, put in the apparatus,Soxhlet's, add water 90ml, to extracting liquid colourless, shift extracting solution with the electric heater heating and refluxing extraction to the 100ml measuring bottle, add water to scale, shake up; Precision measures 10ml, adds ethanol 150ml, shakes up, and places 12h for 4 ℃, takes out, and is centrifugal, the supernatant that inclines, and precipitation is dissolved in water, and is transferred in the 50ml measuring bottle, adds water to scale, shakes up, and get final product.
Algoscopy: precision measures need testing solution 2ml, puts in the 10ml tool plug test tube, and the method under the preparation of sighting target directrix curve from " the accurate sulphuric acid anthrone solution 6ml that adds ", is measured absorbance in accordance with the law, presses the content that standard curve calculates ganoderan.
3, Radix Et Rhizoma Fagopyri Tatarici total flavonoids
The preparation of reference substance solution: be taken at 120 ℃ of control substance of Rutin that are dried to constant weight an amount of, accurately weighed, add 10% methanol and make the solution that every 1ml contains 0.2mg, and get final product.
The preparation of standard curve: precision measures reference substance solution 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, puts respectively in the 25ml measuring bottle, respectively adds water to 6ml, add 5% sodium nitrite solution 1ml, shake up, place 6min, add 10% aluminum nitrate solution 1ml, shake up, place 6min, hydro-oxidation sodium test solution 10ml adds water to scale again, shakes up, place 15min, take corresponding reagent as blank, measure absorbance in the 500nm place, take absorbance as vertical coordinate, concentration is abscissa, the drawing standard curve.
The preparation of need testing solution: get approximately 1g of extract, accurately weighed, put in the apparatus,Soxhlet's, add chloroform 100ml, reflux discards chloroform liquid to extracting liquid colourless, add methanol 90ml, reflux is to extracting liquid colourless, in the dislocation 100ml measuring bottle again, add methanol to scale, shake up, precision measures 10ml, put in the 100ml measuring bottle, add water to scale, shake up, and get final product.
Algoscopy: precision measures need testing solution 3ml, and the method under the preparation of sighting target directrix curve from " adding water to 6ml ", is measured absorbance in accordance with the law, presses the content that standard curve calculates Radix Et Rhizoma Fagopyri Tatarici total flavonoids.
Test to prove advantage of the present invention below by the antioxidant activity of removing hydroxy radical, used extract and composition sample are embodiment 1 prepared extract and compositions thereof in the experiment.
Experimental principle: utilize the Fenton reaction to produce hydroxy radical, hydroxy radical and dimethyl sulfoxine reaction generate formaldehyde, formaldehyde is through 2, generate corresponding hydrazone (HCHO-DNPH) after the 4-dinitrophenylhydrazine is derivative, by adding in the high-efficient liquid phase chromatogram technique analysis reaction system or the variation of HCHO-DNPH peak area when not adding testing sample, record sample to the clearance rate of hydroxy radical, weigh the antioxidant activity of sample with this.
The preparation of reference substance solution: get formaldehyde (37%) reference substance an amount of, precision measures, and adds the solution that water is made 332.7nmol/L, and get final product.
The preparation of 2,4 dinitrophenyl hydrazine solution: get 2,4 dinitrophenyl hydrazine an amount of, accurately weighed, add the solution that acetonitrile is made 10.0mmol/L, and get final product.
Fe
2+The preparation of solution: get ferrous sulfate (FeSO
47H
2O) an amount of, accurately weighed, add the solution that water is made 2.0mmol/L, and get final product.
The preparation of hydrogenperoxide steam generator: get hydrogen peroxide (30%) an amount of, precision measures, and adds the solution that water is made 107.7nmol/L, and get final product.
The preparation of dimethyl sulfoxide solution: get dimethyl sulfoxine an amount of, precision measures, and adds the solution that water is made 225.2nmol/L, and get final product.
The preparation of need testing solution: get test sample 20mg, stable precision is put in the 100ml measuring bottle, adds the acetonitrile dissolving and is diluted to scale, shakes up, and precision measures 5ml, puts in the 10ml measuring bottle, is diluted to scale with water, shakes up, and get final product.
Chromatographic condition: chromatographic column: octadecylsilane chemically bonded silica post Diamonsil C
18(250mm * 4.6mm, 5 μ m); Mobile phase: acetonitrile-water (65: 35); Detect wavelength: 365nm; Flow velocity: 0.8mLmin
-1Sample size: 20 μ l.
Algoscopy: get formaldehyde reference substance solution 1.0ml, put in the tool plug test tube, add 2,4-dinitro benzene hydrazine solution 0.1ml, put in 60 ℃ of water-baths and react 20min, take out, the ice bath cooling, add acetonitrile and make into 5.0ml, shake up accurate 20 μ l, the injection liquid chromatography drawn, measure the retention time at HCHO-DNPH peak, determine the peak position.Get need testing solution 1.0ml, add dimethyl sulfoxide solution, Fe
2+Solution, each 1.0ml of hydrogenperoxide steam generator shake up, and put in 37 ℃ of water-baths and react 30min, take out, add 2,4 dinitrophenyl hydrazine solution 0.5ml, put in 60 ℃ of water-baths and react 20min, take out, the ice bath cooling adds acetonitrile and makes into 5.0ml, shake up, the accurate 20 μ l that draw, the injection liquid chromatography is measured HCHO-DNPH peak area (A
1); Other gets need testing solution 0ml, with the method operation, measures HCHO-DNPH peak area (A
2), calculate Scavenging action to hydroxyl free radical according to following formula: Scavenging action to hydroxyl free radical (%)=(A
2-A
1)/A
2* 100%.Other gets the positive drug ascorbic acid of removing hydroxy radical, adds the solution that water is made 0.5mg/ml, gets 1.0ml, operates, measures and calculate its Scavenging action to hydroxyl free radical, as reference with method.
Table 1 present composition is to the scavenging action of hydroxy radical
*Can't detect HCHO-DNPH in the reaction system of this concentration sample.Because hydroxy radical under this reaction condition is completely removed, to fail to produce formaldehyde, thereby can not generate HCHO-DNPH, its peak area is 0.
As can be seen from Table 1, present composition 0.5mg/ml concentration is comparatively approaching to the ascorbic acid of the clearance rate of hydroxy radical and 0.5mg/ml, and present composition 0.75mg/ml concentration then is higher than the ascorbic acid of 0.5mg/ml to the clearance rate of hydroxy radical; 0.5mg/ml present composition Ganoderma extract, the tartary buckwheat extract sample identical with active material concentration compare, the present composition is higher than the two clearance rate (40.7%, 33.5%) sum when using separately of Ganoderma extract, tartary buckwheat extract to the clearance rate (85.2%) of hydroxy radical, therefore, the Ganoderma extract in the present composition and tartary buckwheat extract have cooperative effect to the clearance rate of hydroxy radical.
Above-mentioned experimental result shows, the present composition can be removed hydroxy radical, has very strong antioxidation, and its successful is better than independent use Ganoderma extract or tartary buckwheat extract.
Below in conjunction with embodiment the present invention is described in further details, but content of the present invention is not limited in this.
The specific embodiment
Embodiment 1
Get the Ganoderma 1.5kg after the pulverizing, 95.0% alcohol reflux 3 times with its 10 times, 8 times, 6 times amount volumes (namely 15 liters, 12 liters, 9 liters), each 1h, behind the extracting solution Recycled ethanol, be splined on the siliceous earth column that the kieselguhr consumption is 2kg, then use the eluent ethyl acetate of 6 times of column volumes, collect eluent, reclaim ethyl acetate, get extract A; Get the Ganoderma medicinal residues behind above-mentioned 95.0% ethanol extraction, with the water boiling and extraction of its 12 times, 10 times amount volumes 2 times, each 1h, it is 1.15 that extracting solution is concentrated into relative density, and then adding 95.0% ethanol is 70% to containing the alcohol amount, after precipitating fully, filter, get filtration cakes torrefaction, get extract B; United extraction thing A and extract B and mix homogeneously get Ganoderma extract 187.8g;
Get the Radix Et Rhizoma Fagopyri Tatarici 1.5kg after the pulverizing, 95.0% alcohol reflux 3 times with its 10 times, 8 times, 6 times amount volumes (namely 15 liters, 12 liters, 9 liters), each 1h behind the extracting solution Recycled ethanol, is splined on the macroporous resin column that resin demand is 2.5kg, follow the washing decontamination with 5 times of column volumes, use again 80% ethanol elution of 8 times of column volumes, collect ethanol elution, Recycled ethanol, concentrated, dry, get tartary buckwheat extract 48.5g;
With above-mentioned Ganoderma extract and tartary buckwheat extract mix homogeneously, namely get Ganoderma extract and tartary buckwheat extract compositions, wherein contain Ganoderma total triterpenes 4.9%, ganoderan 17.7%, Radix Et Rhizoma Fagopyri Tatarici total flavonoids 19.1%.
Embodiment 2
Get the Ganoderma 1.5kg after the pulverizing, 85.0% ethanol percolate extraction with its 25 times amount volumes (namely 37.5 liters), behind the extracting solution Recycled ethanol, being splined on the kieselguhr consumption is the 1.8kg siliceous earth column, then use the eluent ethyl acetate of 5 times of column volumes, collect eluent, reclaim ethyl acetate, get extract A; Get the Ganoderma medicinal residues behind above-mentioned 85.0% ethanol extraction, with the water boiling and extraction of its 15 times, 12 times amount volumes 2 times, each 1h, it is 1.18 that extracting solution is concentrated into relative density, and then adding 95.0% ethanol is 65% to containing the alcohol amount, after precipitating fully, filter, get filtration cakes torrefaction, get extract B; United extraction thing A and extract B and mix homogeneously get Ganoderma extract 192.6g;
Get the Radix Et Rhizoma Fagopyri Tatarici 1.5kg after the pulverizing, 85.0% ethanol percolate extraction with its 22 times amount volumes (namely 33 liters), behind the extracting solution Recycled ethanol, be splined on the macroporous resin column that resin demand is 3.2kg, then with the washing decontamination of 4 times of column volumes, use again 75% ethanol elution of 10 times of column volumes, collect ethanol elution, Recycled ethanol, concentrated, dry, get tartary buckwheat extract 51.3g.
With above-mentioned Ganoderma extract and tartary buckwheat extract mix homogeneously, namely get Ganoderma extract and tartary buckwheat extract compositions, wherein contain Ganoderma total triterpenes 5.4%, ganoderan 18.2%, Radix Et Rhizoma Fagopyri Tatarici total flavonoids 22.0%.
Embodiment 3
Get the Ganoderma 1.5kg after the pulverizing, 95.0% alcohol reflux 3 times with its 8 times, 8 times, 6 times amount volumes (namely 12 liters, 12 liters, 9 liters), each 1h, behind the extracting solution Recycled ethanol, be splined on the siliceous earth column that the kieselguhr consumption is 1.8g, then use the eluent ethyl acetate of 4 times of column volumes, collect eluent, reclaim ethyl acetate, get extract A; Get the Ganoderma medicinal residues behind above-mentioned 95.0% ethanol extraction, with the water boiling and extraction of its 10 times, 10 times amount volumes 2 times, each 1h, it is 1.13 that extracting solution is concentrated into relative density, and then adding 95.0% ethanol is 70% to containing the alcohol amount, after precipitating fully, filter, get filtration cakes torrefaction, get extract B; United extraction thing A and extract B and mix homogeneously get Ganoderma extract 176.9g;
Get the Radix Et Rhizoma Fagopyri Tatarici 1.5g after the pulverizing, with 20 times of amount 95.0% ethanol percolate extraction, behind the extracting solution Recycled ethanol, be splined on macroporous resin column (resin demand is 3.2kg), use first the washing decontamination of 5 times of column volumes, use again 80% ethanol elution of 8 times of column volumes, collect ethanol elution, Recycled ethanol, concentrated, dry, get tartary buckwheat extract 49.7g.
With above-mentioned Ganoderma extract and tartary buckwheat extract mix homogeneously, namely get Ganoderma extract and tartary buckwheat extract compositions, wherein contain Ganoderma total triterpenes 4.3%, ganoderan 16.8%, Radix Et Rhizoma Fagopyri Tatarici total flavonoids 20.6%.
Embodiment 4
Get embodiment 1 resulting composition 100g, add dextrin 200g mix homogeneously, the granule processed take 95% ethanol as binding agent, drying, granulate, gets the lucid ganoderma bitter buckwheat capsule by encapsulated 1000.
Embodiment 5
Get embodiment 2 resulting composition 100g, with sucrose 20g, Mel 20g, citric acid 2g, make 1000ml with edible distillate spirit, get lucid ganoderma bitter buckwheat wine.
Claims (3)
1. the compositions of a Ganoderma extract and tartary buckwheat extract is characterized in that: contained Ganoderma extract and tartary buckwheat extract are extracted by the Ganoderma of equal in quality and Radix Et Rhizoma Fagopyri Tatarici and make,
Wherein Ganoderma extract makes by the following method:
(1) first Ganoderma is pulverized, then with 70.0%～95.0% alcohol reflux of 5～10 times of amount volumes of Ganoderma quality 3 times, each 1h, or with 70.0%～95.0% ethanol percolate extraction of 15～25 times of Ganoderma quality amount volumes, behind the extracting solution Recycled ethanol with reflux, extract, or percolation extraction, be splined on siliceous earth column, used diatomaceous quality is 1～1.5 times of Ganoderma quality, then use the eluent ethyl acetate of 4～6 times of column volumes, collect eluent, reclaim ethyl acetate, get extract A;
(2) get Ganoderma medicinal residues behind above-mentioned 70.0%～95.0% ethanol extraction, with water boiling and extraction 2 times, each 10～15 times of water extraction 1h that measure volumes with Ganoderma medicinal residues quality, it is 1.10～1.20 that extracting solution is concentrated into relative density, then adding 95.0% ethanol is 60%～70% to containing the alcohol amount, after precipitating fully, filters, get filtration cakes torrefaction, get extract B;
(3) united extraction thing A and extract B, and mix homogeneously namely get Ganoderma extract;
Wherein tartary buckwheat extract makes by the following method:
Radix Et Rhizoma Fagopyri Tatarici is pulverized, then with 70.0%～95.0% alcohol reflux of 5～10 times of amount volumes of Radix Et Rhizoma Fagopyri Tatarici quality 3 times, each 1h, or with 70.0%～95.0% ethanol percolate extraction of 15～25 times of Radix Et Rhizoma Fagopyri Tatarici quality amount volumes, behind the extracting solution Recycled ethanol with reflux, extract, or percolation extraction, be splined on macroporous resin column, used resin quality is 1.5～2.5 times of Radix Et Rhizoma Fagopyri Tatarici quality, follow the washing decontamination with 3～5 times of column volumes, use again 50.0%～80.0% ethanol elution of 5～10 times of column volumes, collect ethanol elution, Recycled ethanol, concentrated, drying namely gets tartary buckwheat extract.
2. the compositions of Ganoderma extract according to claim 1 and tartary buckwheat extract is characterized in that: said composition adds the excipient of pharmaceutically accepting and makes capsule, tablet, pill, granule, oral liquid or medicated wine.
3. the compositions of Ganoderma extract as claimed in claim 1 and tartary buckwheat extract is in the medicine for preparing prevention or the treatment disease relevant with oxidative damage or the application in the health product.
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| CN103505507A (en) * | 2012-06-28 | 2014-01-15 | 天津天狮生物发展有限公司 | Traditional Chinese medicine composition containing glossy ganoderma and preparation method of composition |
| CN102994350B (en) * | 2012-12-21 | 2014-01-01 | 劲牌有限公司 | Preparation method of tartary buckwheat wine with function of reducing blood fat |
| CN104338003A (en) * | 2013-07-29 | 2015-02-11 | 刘庆山 | Preparation method and application for medicine with liver-protecting kidney-protecting efficacy and good mouthfeel |
| WO2016049811A1 (en) * | 2014-09-29 | 2016-04-07 | 深圳市仁泰生物科技有限公司 | Antitumour composition rich in triterpenoids and preparation method thereof |
| CN104872728A (en) * | 2015-05-08 | 2015-09-02 | 苏州葛家坞生物科技有限公司 | Preparation method of lucid ganoderma crab paste and product of preparation method |
| CN107027925A (en) * | 2017-03-27 | 2017-08-11 | 成都大学 | Ganoderma lucidum Assorted Chinese Herbal Tea and preparation method thereof |
| CN107115366A (en) * | 2017-06-15 | 2017-09-01 | 贵州黔芝灵绿色农业科技发展有限公司 | A kind of extracting method of ganoderma lucidum |
| CN107556393A (en) * | 2017-08-07 | 2018-01-09 | 华南理工大学 | A kind of hot water of the ganoderma lucidum dregs of a decoction removes sugared method and product |
| CN113358788A (en) * | 2021-06-09 | 2021-09-07 | 劲牌有限公司 | Method for identifying authenticity of tartary buckwheat wine based on fingerprint spectrum |
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