CN102100760A - Plant extract composition capable of preventing and treating sugar metabolism disturbance and preparation method thereof - Google Patents
Plant extract composition capable of preventing and treating sugar metabolism disturbance and preparation method thereof Download PDFInfo
- Publication number
- CN102100760A CN102100760A CN2011100267856A CN201110026785A CN102100760A CN 102100760 A CN102100760 A CN 102100760A CN 2011100267856 A CN2011100267856 A CN 2011100267856A CN 201110026785 A CN201110026785 A CN 201110026785A CN 102100760 A CN102100760 A CN 102100760A
- Authority
- CN
- China
- Prior art keywords
- extract
- composition
- carbohydrate metabolism
- plant extracts
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/718—Coptis (goldthread)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Diabetes (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Botany (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Emergency Medicine (AREA)
- Endocrinology (AREA)
- Epidemiology (AREA)
- Hematology (AREA)
- Obesity (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a plant extract composition capable of preventing and treating sugar metabolism disturbance, comprising a panax notoginseng saponins (PNS) extract and a total coptis alkaloids(TCA) extract. The invention also discloses the preparation method of the plant extract composition, comprising the following steps: extracting pseudo-ginseng by C1-3 alcohol, mixing the total extract, concentrating to obtain the concentrated liquid of the total extract, and removing non-saponin components to obtain the PNS extract; adding C1-3 alcohol into rhizoma coptidis, refluxing and extracting, recovering C1-3 alcohol, then concentrating, using acid to dissolve alkali precipitation in the concentrated liquid and recrystallizing to separate the TCA extract; and mixing the PNS extract with the TCA extract to obtain the plant extract composition capable of preventing and treating sugar metabolism disturbance. According to the invention, a great amount of ineffective chemical components in the primary compound traditional Chinese medicine are removed, thereby reducing the influences of the ineffective components on product processing as well as the quality and security of the preparation of the composition. The effective components are explicit, the preparation process is stale, and the product quality is controllable, thereby being beneficial to the industrial production, significantly improving the blood sugar lowering effect, and obtaining the more stable and better effect on treating sugar metabolism disturbance diseases.
Description
Technical field
The present invention relates to Chinese medicine extract, be specifically related to a kind of composition of plant extracts of preventing and treating carbohydrate metabolism disturbance and preparation method thereof.
Background technology
" the loyal art of compound recipe is transferred fat side " (FTZ), is a kind of Chinese medicine composition, is used for the treatment of hyperlipemia, obtains desirable curative effect, and clinical total effective rate reaches 91%.Full side is made up of Fructus Ligustri Lucidi, the Rhizoma Atractylodis Macrocephalae, Fructus Citri Sarcodactylis, the Cortex Eucommiae, Radix Cirsii Japonici, Radix Salviae Miltiorrhizae, Rhizoma Coptidis, Radix Notoginseng 8 flavor Chinese medicines.This prescription FTZ has obtained the national inventing patent mandate, the patent No.: 200410051250.4.
Though many herbal mixtures have good blood lipid regulation and arteriosclerosis function and clinical efficacy, but its beneficial effect to relevant diseases such as carbohydrate metabolism syndrome such as diabetes is not seen open, and owing to be compound Chinese medicinal preparation, also have many weak points: (1) its composition is complicated, its effective ingredient is not clear, be difficult for quantizing, influence the stability of the clinical efficacy of product the most at last, (2) simple relatively preparation technology, effective ingredient can not get due purification in the side, and impurity component is many, taking dose is big, it is not ideal enough that patient takes compliance, and (3) because prescription extract amount is big, range of application is subjected to various restrictions.
Summary of the invention
The objective of the invention is to overcome the deficiency of prior art, provide a kind of more remarkable treatment effect definite, the effective component content height is easy to quantize the composition of plant extracts of the control carbohydrate metabolism disturbance that quality is easy to control.
Another object of the present invention provides a kind of process stabilizing, the preparation method of the composition of plant extracts of the above-mentioned control carbohydrate metabolism disturbance of favorable reproducibility.
Another purpose of the present invention provides the composition of plant extracts of above-mentioned control carbohydrate metabolism disturbance and uses in preparation prevention or treatment carbohydrate metabolism disturbance relevant disease medicine.
The present invention is achieved through the following technical solutions above-mentioned purpose:
A kind of composition of plant extracts of preventing and treating carbohydrate metabolism disturbance comprises extract of panax notoginseng saponins and Rhizoma Coptidis total alkaloids extract.The weight ratio of extract of panax notoginseng saponins and Rhizoma Coptidis total alkaloids extract is preferably 1 ~ 9:1 ~ 3.
A kind of preparation method of preventing and treating the composition of plant extracts of carbohydrate metabolism disturbance, step is as follows:
(1) preparation of extract of panax notoginseng saponins: with material medicine Radix Notoginseng process C
1-3After the alcohol extraction, merge total extract, obtain concentrated solution behind the concentrated total extract, remove non-saponin component in the concentrated solution, obtain extract of panax notoginseng saponins;
(2) preparation of Rhizoma Coptidis total alkaloids extract: the material medicine Rhizoma Coptidis is added C
1-3Alcohol reflux extracts, and reclaims C
1-3Alcohol, concentrate concentrated solution, separate out the Rhizoma Coptidis total alkaloids extract with acid dissolving alkali precipitation recrystallization again;
(3) extract of panax notoginseng saponins is mixed with the Rhizoma Coptidis total alkaloids extract, obtain preventing and treating the composition of plant extracts of carbohydrate metabolism disturbance.
Above-mentioned preparation method step (1) C
1-3Alcohol extraction is the C with 30～95 volume %
1-3The C of 65～75 volume % is preferably used in alcohol extraction 1～5 time
1-3Alcohol extraction 2～3 times, each C that extracts
1-3The alcohol volume is 1～15 times of quality of medicinal material, is preferably 5～10 times, and each extraction time is more than 5 minutes, is preferably 60 ~ 120 minutes; Described C
1-3Alcohol is methanol, ethanol or propanol, is preferably ethanol, because the ethanol safety is higher than methanol, and also easier acquisition, extraction effect is also good than propanol.
Remove non-saponin component employing macroporous resin in the concentrated solution in the step (1), the amount ratio of macroporous resin and concentrated solution is that the 1kg resin adds concentrated solution 0.5 ~ 5L, is preferably 1 ~ 1.5L.With 50 ~ 95 volume % ethanol, be preferably 75 ~ 85 volume % ethanol with macroporous resin column absorption back eluting, consumption is every 1kg macroporous resin with 3 ~ 20 L ethanol, preferred 4 ~ 6 L ethanol, with 1 ~ 20 ml/min, preferred 5 ~ 12 ml/min flow velocity eluting, be eluted to colourless; Described macroporous resin is polyamides amine type resin or polystyrene weak-base anion-exchange resin.
Step (2) C
1-3It is with 50 ~ 95 volume % that alcohol reflux extracts, and is preferably 65 ~ 75 volume % C
1-3Alcohol extraction 1～5 time is preferably 2～3 times, each C that extracts
1-3The alcohol volume is 1～15 times of quality of medicinal material, is preferably 5～10 times, and each extraction time is more than 5 minutes, is preferably 60 ~ 120 minutes.Described C
1-3Alcohol is methanol, ethanol or propanol, is preferably ethanol, because the ethanol safety is higher than methanol, and also easier acquisition, extraction effect is also good than propanol.
The acid dissolving is to use acetate dissolution, and the acetic acid consumption is C
1-33～10 times of ethanol extract are preferably 3～5 times; Add 1 ~ 30 quality %, being preferably 10 quality %HCl accent pH is 0.2 ~ 4, and preferred pH is 1.5 ~ 2.5.Add the NaCl recrystallization, solution NaCl concentration is about 5～12 quality %, is preferably 6～8 quality %; Alkali precipitation is with 1 ~ 30 quality %, is preferably 10 quality %NaOH solution, and pH is 9 ~ 13, and preferred pH is 11 ~ 12.5, and the recrystallization volume is 1 ~ 5 times of Rhizoma Coptidis weight, is preferably 2 ~ 3 times.
The application of the composition of plant extracts of control carbohydrate metabolism disturbance in preparation prevention or treatment carbohydrate metabolism disturbance disease medicament or health product, described carbohydrate metabolism disturbance disease is hyperglycemia or diabetes.
Described medicine for composition of plant extracts that the present invention is prevented and treated carbohydrate metabolism disturbance according to acceptable carrier and/or adjuvant on the pharmaceutics, use general formulation method, be processed into forms such as oral formulations or ejection preparation.Conventional adjuvant has lubricant, filler, binding agent, disintegrating agent etc.Oral formulations has tablet, capsule, powder, pill, powder, granule, crystal, solution, extractum, outstanding agent, soup, syrup, elixir, tea, wet goods form.
The composition of plant extracts effective ingredient of control carbohydrate metabolism disturbance of the present invention is arasaponin R1, ginsenoside Rb1, ginsenoside Rd, ginsenoside Re, ginsenoside Rg1, berberine, coptisine, epiberberine, palmatine.The wherein wt ratio is an arasaponin R1: ginsenoside Rb1: ginsenoside Rd: ginsenoside Re: ginsenoside Rg1: berberine: coptisine: epiberberine: palmatine equals 1～4:1～8:1～3:1～2:1～11:1～10:1～3:1～2:1～3.
When the proportioning of extract of panax notoginseng saponins and yellow total alkaloids extract was 7:3, the composition of plant extracts effective ingredient and the weight percentage ratio of control carbohydrate metabolism disturbance are respectively: arasaponin R1: ginsenoside Rg1: ginsenoside Re: ginsenoside Rb1: ginsenoside Rd: berberine: coptisine: epiberberine: palmatine equaled 2.3～2.8:10～12:0.95～1.1:7.6～9.0:1.6～1.9:9.5～11.5:2.4～2.8:1.1～1.3:2.1～2.4.
Through pharmacological research, animal vivo test shows, the composition of plant extracts that the present invention prevents and treats carbohydrate metabolism disturbance has good hypoglycemic activity to the experimental rat diabetes, and it is similar to the blood sugar lowering effect of insulin, also has the effect that improves insulin resistant, definite for carbohydrate metabolism syndrome clinical efficacies such as treatment diabetes, and do not observe apparent side effect.
Compared with prior art, the present invention has following beneficial effect:
The composition of plant extracts of control carbohydrate metabolism disturbance of the present invention is on Chinese invention patent ZL200410051250.4 basis, choose two flavor Chinese medicine Radix Notoginseng and Rhizoma Coptidis wherein, with the compositions that its extract is formed, said composition is the best compatibility that obtains at the screening active ingredients that carries out active component group (effective site) and component compatibility thereof.By preparation method of the present invention, can remove a large amount of invalid chemical constituents in the herbal mixture, obtain the effective extract part of more effective Chinese medicine, both kept the Chinese medicine characteristic, the active constituent content in the Chinese medicine is improved greatly, reduced invalid components product processing and the quality of the pharmaceutical preparations and Influence on security, make this herbal mixture effective ingredient clear and definite, stable preparation process, controllable product quality helps mass production.Animal vivo test shows that this composition of plant extracts has tangible blood sugar reducing function to hyperglycemia, and does not observe apparent side effect, has increased the safety of medication.
The invention has the advantages that to preventing and/or treating the carbohydrate metabolism disturbance relevant disease a kind of new product is provided.The medicine of the present invention and similar control carbohydrate metabolism disturbance relevant disease relatively, determined curative effect, the no obvious toxic-side effects of safety, use or good prospects for application all arranged separately with other medicines are composite.
The loyal art accent of the composition of plant extracts of control carbohydrate metabolism disturbance of the present invention and compound recipe fat side (former side, FTZ) compare:
The composition of plant extracts of control carbohydrate metabolism disturbance of the present invention is compared with former side FTZ:
(1) dose is reduced to 600mg by original 6g extract, is reduced to original 1/10;
(2) content of effective ingredient improves about 10 times;
(3) animal vivo test shows; this composition of plant extracts has obvious blood sugar reducing function to experimental diabetic animal; and can improve insulin and carry anti-; protection islet cells, promotion insulin secretion, increase serum insulin content; significant blood sugar lowering is arranged, improve the blood glucose metabolism; and do not observe apparent side effect; increased the safety of medication; blood glucose metabolism disorder relevant disease clinical efficacies such as treatment hyperglycemia and diabetes are definite; this extractive composition part index number is better than former side, has reached the purpose that reduces dose and do not reduce drug effect.
Description of drawings
The chromatographic peak figure of composition of plant extracts Radix Notoginseng total arasaponins composition in the finger printing that the HPLC analysis obtains of control carbohydrate metabolism disturbance under the preparation of preparation (6) tablet of the composition of plant extracts of Fig. 1: embodiment 1 control carbohydrate metabolism disturbance.
The chromatographic peak figure of composition of plant extracts Rhizoma Coptidis total alkaloids composition in the finger printing that the HPLC analysis obtains of control carbohydrate metabolism disturbance under the preparation of preparation (6) tablet of the composition of plant extracts of Fig. 2: embodiment 1 control carbohydrate metabolism disturbance.
The specific embodiment
The preparation of the composition of plant extracts of embodiment 1 control carbohydrate metabolism disturbance
One, the preparation of extract of panax notoginseng saponins
(1) extract of panax notoginseng saponins
Take by weighing 10 kg pseudo-ginsengs, pulverize, distinguish reflux, extract, 3 times with 8 times, 8 times of medical material amount and 6 times 75 volume % ethanol, each 2h, merge extractive liquid, filters with 200 order filter clothes, merging filtrate.Reclaiming ethanol and making alcohol extraction part concentration is the concentrated solution (1.9 L) that 1ml is equivalent to the 1g crude drug; Last macroporous resin column absorption, macroporous resin is the HPD-100 of polystyrene weak-base anion-exchange resin series, add concentrated solution 1.5L by 1 kg resin and go up macroporous resin column absorption, wash with water to eluent closely colourless, use 85 volume % ethanol, 4.5 L with 10 ml/min flow velocity eluting then, it is colourless to be eluted to effluent, merge eluent, vacuum decompression recovery ethanol and concentrated most 1ml are equivalent to 2 g crude drug concentrated solutions approximately, and (relative density is 1.08～1.10,60 ℃), dry total saponin extracts 0.65 kg that gets below 60 ℃.
Exsiccant method is a lot, available drying under reduced pressure, also available thin film evaporation drying etc.The present invention preferably adopts spray drying method, 80 ~ 108 ℃ of its intake air temperatures, 55 ~ 60 ℃ of outlet temperatures, wind speed 5 ~ 10m/s, pressure 0.02 ~ 0.1kPa, 1 ~ 2 drying.
It is 73.8% that extract of panax notoginseng saponins contains content of the total saponins in radix notoginseng with HPLC method mensuration, and the content of ginsenoside Rb1, ginsenoside Rd, ginsenoside Re, ginsenoside Rg1, arasaponin R1 is respectively 25.6%, 5.2%, 3.1%, 32.1%, 7.5% of extract gross weight.
(2) extract of panax notoginseng saponins
Take by weighing 10 kg pseudo-ginsengs, pulverize, distinguish reflux, extract, 3 times with 8 times, 7 times of medical material amount and 5 times 65 volume % methanol, each 2h, merge extractive liquid, filters with 200 order filter clothes, merging filtrate.Reclaiming methanol and making alcohol extraction part concentration is the concentrated solution (2.0 L) that 1ml is equivalent to the 1g crude drug; Last macroporous resin column absorption, macroporous resin is the HPD-600 of polystyrene weak-base anion-exchange resin series, add concentrated solution 1.2 L by 1 kg resin and go up macroporous resin column absorption, wash with water to eluent closely colourless, use 90 volume % ethanol, 3.5 L with 10 ml/min flow velocity eluting then, it is colourless to be eluted to effluent, merge eluent, vacuum decompression recovery ethanol and concentrated most 1ml are equivalent to 2 g crude drug concentrated solutions approximately, and (relative density is 1.08～1.10,60 ℃), drying under reduced pressure below 60 ℃ gets total saponin extracts 0.64 kg.
It is 75.2% that extract of panax notoginseng saponins contains content of the total saponins in radix notoginseng with HPLC method mensuration, and the content of ginsenoside Rb1, ginsenoside Rd, ginsenoside Re, ginsenoside Rg1, arasaponin R1 is respectively 26.1%, 5.3%, 3.2%, 32.8%, 7.6% of extract gross weight.
(3) extract of panax notoginseng saponins
Take by weighing 10 kg pseudo-ginsengs, pulverize, distinguish reflux, extract, 2 times with 8 times, 7 times 70 volume % normal propyl alcohols of medical material amount, each 2h, merge extractive liquid, filters with 200 order filter clothes, merging filtrate.Reclaiming normal propyl alcohol and making alcohol extraction part concentration is the concentrated solution (1.7 L) that 1ml is equivalent to the 1g crude drug; Last macroporous resin column absorption, macroporous resin is preferably polyamide type AB-8.Add concentrated solution 1.3L by 1 kg resin and go up macroporous resin column absorption, wash with water to eluent closely colourless, the back with 80% ethanol, 6 L with 9 ml/min flow velocity eluting, be eluted to effluent colourless, the merging eluent, vacuum decompression recovery ethanol and concentrated most 1ml are equivalent to 2 g crude drug concentrated solutions approximately, and (relative density is 1.08～1.10,60 ℃), drying under reduced pressure below 60 ℃ gets total saponin extracts 0.62 kg.
It is 72.2% that extract of panax notoginseng saponins contains content of the total saponins in radix notoginseng with HPLC method mensuration, and the content of ginsenoside Rb1, ginsenoside Rd, ginsenoside Re, ginsenoside Rg1, arasaponin R1 is respectively 25.3%, 5.0%, 3.2%, 31.3%, 7.2% of extract gross weight.
Table 1 Different Preparation extract of panax notoginseng saponins yield and HPLC assay result (%)
| The sample sequence number | Yield | Radix Notoginseng total arasaponins | The ginsenoside Rb1 | The ginsenoside Rd | The ginsenoside Re | The ginsenoside Rg1 | Arasaponin R1 |
| （1） | 6.5 | 73.8 | 25.6 | 5.2 | 3.1 | 32.1 | 7.5 |
| （2） | 6.4 | 75.2 | 26.1 | 5.3 | 3.2 | 32.8 | 7.6 |
| （3） | 6.2 | 72.2 | 25.3 | 5.0 | 3.2 | 31.3 | 7.2 |
Two, the preparation of Rhizoma Coptidis total alkaloids
(4) Rhizoma Coptidis total alkaloids extract
Take by weighing 10 kg Rhizoma Coptidis medical materials, coarse pulverization is distinguished reflux, extract, 3 times with 6 times, 5 times of medical material amount and 5 times 70% ethanol, each 2 h, and merge extractive liquid,, vacuum decompression reclaims ethanol and is concentrated into 1.3g medical material, ml, gets fluid extract 2.1kg.Add glacial acetic acid 6 L dissolving, fully stir, 4～10 ℃ of placements are spent the night, centrifugal (5000 r/min, 15min), sucking filtration, discard precipitation, get supernatant, add 10 quality %HCl in the supernatant and transfer pH to 2.5, add NaCl then, make solution NaCl concentration be about 8 quality %, fully stir, make the NaCl dissolving, leave standstill 24h, centrifugal (5000 r/min, 15min), collecting precipitation thing; Supernatant adds 10 quality %NaOH and transfers pH to 12, leaves standstill 24h, centrifugal (5000 r/min, 15min), the collecting precipitation thing merges 2 times precipitate, and 60 ℃ of drying under reduced pressure promptly get Rhizoma Coptidis total alkaloids extract 1.65 kg.
It is 57.1% that the Rhizoma Coptidis total alkaloids extract contains total alkaloid content with HPLC method mensuration, and wherein berberine 35.0%, coptisine 8.6%, epiberberine 4.1%, palmatine 7.6%.
(5) Rhizoma Coptidis total alkaloids extract
Take by weighing 10 kg Rhizoma Coptidis medical materials, coarse pulverization is distinguished reflux, extract, 3 times with 8 times, 5 times of medical material amount and 4 times 75 volume % methanol, each 2 h, and merge extractive liquid,, vacuum decompression reclaims methanol and gets extractum 2.05kg.Add glacial acetic acid and dissolve 5.5 L, get acid water extracting liquid, 4 ~ 10 ℃ of placements are spent the night, centrifugal (5000 r/min, 15min), sucking filtration, discard precipitation, get supernatant, add 10 quality %HCl in the supernatant and transfer pH to 3.0, add NaCl then, make solution NaCl concentration be about 6 quality %, fully stir, make the NaCl dissolving, leave standstill 24h, centrifugal (5000 r/min, 15min), collecting precipitation thing; Supernatant adds 10 quality %NaOH and transfers pH to 12, leaves standstill 24h, centrifugal (5000 r/min, 15min), the collecting precipitation thing merges 2 times precipitate, and 60 ℃ of drying under reduced pressure promptly get Rhizoma Coptidis total alkaloids extract 1.63 kg.
It is 55.6% that the Rhizoma Coptidis total alkaloids extract contains total alkaloid content with HPLC method mensuration, and wherein berberine 34.0%, coptisine 8.4%, epiberberine 4.0%, palmatine 7.5%.
The Radix Notoginseng total arasaponins chromatographic condition:
With the octadecylsilane chemically bonded silica is filler; With the acetonitrile is mobile phase A, is Mobile phase B with water, and the regulation in the according to the form below 2 is carried out gradient elution; The detection wavelength is 203nm; Flow velocity: 1ml/min; Column temperature: 25 ℃; Detect wavelength: 203nm; The automatic sampler sample introduction.
Table 2
| Time (min) | Mobile phase A (%) | Mobile phase B (%) |
| 0～12 | 19 | 81 |
| 12～60 | 19→36 | 81→64 |
| 60～70 | 36 | 64 |
The Rhizoma Coptidis total alkaloids chromatographic condition:
Acetonitrile-0.05 mol/L potassium dihydrogen phosphate (47:53); Flow velocity: 1 mL/min; Detect wavelength: 345nm.
Four, the preparation of the composition of plant extracts of control carbohydrate metabolism disturbance
(6) preparation of tablet
Take by weighing Rhizoma Coptidis total alkaloids extract 3 kg that are equipped with extract of panax notoginseng saponins 7kg, the preparation of (4) method by above-mentioned (1) legal system, must prevent and treat the composition of plant extracts 10kg of carbohydrate metabolism disturbance by equivalent multiplication method mix homogeneously.
The composition of plant extracts of above-mentioned control carbohydrate metabolism disturbance is analyzed with HPLC, gets accompanying drawing 1, Fig. 2 finger printing.Effective ingredient and weight percentage ratio are: arasaponin R1: ginsenoside Rg1: ginsenoside Re: ginsenoside Rb1: ginsenoside Rd: berberine: coptisine: epiberberine: palmatine equals 2.66:11.2:1.05:8.40:1.75:10.5:2.61:1.26:2.31.
The composition of plant extracts 20kg that gets the control carbohydrate metabolism disturbance adds starch 4kg, dextrin 1kg, 65% syrup 1kg, mixing, it is an amount of to add 80 volume % ethanol, make granule, drying adds the 60g magnesium stearate and mixes thoroughly, is pressed into 200,000, coating promptly gets 0.125g/ sheet (every contains the composition of plant extracts dry powder 100mg that prevents and treats carbohydrate metabolism disturbance).
Measure composition of plant extracts sheet each active constituent content result such as table 3 of control carbohydrate metabolism disturbance with the HPLC method.
The composition of plant extracts sheet HPLC assay result of table 3 control carbohydrate metabolism disturbance
| Test item | Content (mg/ sheet) | Test item | Content (mg/ sheet) |
| The ginsenoside Rb1 | 8.40 | Berberine | 10.5 |
| The ginsenoside Rd | 1.75 | Coptisine | 2.61 |
| The ginsenoside Re | 1.05 | Epiberberine | 1.26 |
| The ginsenoside Rg1 | 11.2 | Palmatine | 2.31 |
| Arasaponin R1 | 2.66 | ? | ? |
(7) preparation of injection
Take by weighing Rhizoma Coptidis total alkaloids extract 1.5 kg that are equipped with extract of panax notoginseng saponins 3kg, the preparation of (4) method by above-mentioned (2) legal system, must prevent and treat the composition of plant extracts 4.5kg of carbohydrate metabolism disturbance by equivalent multiplication method mix homogeneously.
The composition of plant extracts of getting the control carbohydrate metabolism disturbance removes thermal source according to a conventional method, makes injectable powder.
(8) preparation of capsule
Take by weighing the Rhizoma Coptidis total alkaloids extract 4kg that is equipped with extract of panax notoginseng saponins 6kg, the preparation of (5) method by above-mentioned (1) legal system, must prevent and treat the composition of plant extracts 10kg of carbohydrate metabolism disturbance by equivalent multiplication method mix homogeneously.
Processing method: the composition of plant extracts dry powder 10.0k g that gets above-mentioned control carbohydrate metabolism disturbance adds dextrin 5.0kg, mixing, and 100,000 of fill capsules promptly get the 0.15g/ grain, and every contains the composition of plant extracts dry powder 100mg that prevents and treats carbohydrate metabolism disturbance.
(9) preparation of granule
Take by weighing Rhizoma Coptidis total alkaloids extract 0.5 kg that is equipped with extract of panax notoginseng saponins 2 kg, the preparation of (5) method by above-mentioned (2) legal system, must prevent and treat composition of plant extracts 2.5 kg of carbohydrate metabolism disturbance by equivalent multiplication method mix homogeneously.
Get composition of plant extracts 1 kg of control carbohydrate metabolism disturbance, with powdered sugar 18.75 kg, 25% dextrin is starched 1 kg, mixing, it is an amount of to add 80 volume % ethanol, make granule, dry, the machine of going up is packaged into 10,000 parcels, promptly gets 2.0g/ bag (whenever comprising the composition of plant extracts dry powder 100mg that prevents and treats carbohydrate metabolism disturbance).
(10) preparation of pill
Take by weighing Rhizoma Coptidis total alkaloids extract 1 kg that is equipped with extract of panax notoginseng saponins 1 kg, the preparation of (4) method by above-mentioned (3) legal system, must prevent and treat the composition of plant extracts 2kg of carbohydrate metabolism disturbance by equivalent multiplication method mix homogeneously.
Add starch 2 kg, mixing adds the general ball of Mel 2.5kg and becomes water pill, and the dry dry ball of 5kg that gets is packaged into 20,000 parcels, promptly gets 0.25g/ bag (whenever comprising the composition of plant extracts 100mg that prevents and treats carbohydrate metabolism disturbance).Oral, one time 1 parcel, 3 times on the one.
The present invention preferably prevents and treats the composition of plant extracts prescription of carbohydrate metabolism disturbance: parts by weight are 9 ~ 7 parts of extract of panax notoginseng saponins, 1 ~ 3 part of Rhizoma Coptidis total alkaloids extract.It is different that the foregoing description is the drug component proportioning, and formulation method is made various dosage forms routinely, and all have effect of the present invention.
The composition of plant extracts of embodiment 2 control carbohydrate metabolism disturbances is to the effect of normal mouse blood sugar
Animal: 60 of healthy NIH mices, male and female half and half, body weight are 18～22 g.
Grouping: be divided into 5 groups at random by sex: the blank group, the glibenclamide group, the loyal art of compound recipe is transferred the basic, normal, high dosage group of composition of plant extracts (being called for short low dose group, middle dosage group, high dose group) of fat side's group (FTZ group), control carbohydrate metabolism disturbance, 10 every group.
Experimental technique: behind animal fasting 12 h, get blood from the eye socket venous plexus, centrifugal 10 min of blood sample 3000 rpm, separation of serum by the operation of glucose kit description, is measured blood sugar content.Get and irritate stomach immediately behind the blood and give to supply accordingly the reagent thing, glibenclamide 50 mg/kg wherein, the blank group gavages the co-content normal saline, and each administration volume is 0.2 mL/10g body weight.After administration when 3 h, 6 h and 9 h, measuring blood sugar of blood extracting content as stated above respectively, experimental result sees Table 4.
Result's credit by statistics analyses and selects the t method of inspection for use, relatively the difference of each administration group and matched group.
The composition of plant extracts of table 4 control carbohydrate metabolism disturbance is to the effect of normal mouse blood sugar (n=10 x ± s))
Annotate: compare with normal group,
*P＜0.05,
*P＜0.01.
Experimental result shows, with the normal mouse ratio, in administration positive control drug glibenclamide group, FTZ group and each group of basic, normal, high dosage group after 3 hours; After 3,6,9 hours, glibenclamide group, FTZ group and each group of basic, normal, high dosage group have significant reduction effect to the blood glucose of normal mouse.Each organize administration after 6 hours hypoglycemic effect the most remarkable.
The composition of plant extracts of embodiment 3 control carbohydrate metabolism disturbances is to the influence of the blood glucose of diabetic mice
Medicine and reagent: the composition of plant extracts of control carbohydrate metabolism disturbance; Blood glucose test kit (the safe reagent company limited of Beijing northization); T-CHOL, triglyceride, determine cholesterol with high density lipoprotein test kit; Streptozotocin (STZ), U.S. Sigma company product; The peace of quenching one's thirst capsule, Tonghua Baishan Pharmaceutical Ltd's product.Adopt Advantage electronic induction blood glucose meter and supporting paper slip (Switzerland Roche company product) to measure blood glucose.
Experimental subject is to economize the cleaning level NIH mice that Experimental Animal Center provides, male and female half and half, body weight 18～22 g by Nanfang Medical Univ.
(1) to the influence of the blood glucose of streptozotocin (STZ) type diabetic mice
With 80 healthy NIH mices, numbering is divided into 8 groups after weighing at random, 10 every group, gets wherein 1 group as the normal control group.All the other respectively organized the mice fasting after 16 hours, the freshly prepared 1% streptozotocin solution of lumbar injection 150 mg/kg, after 72 hours, the ophthalmic corner of the eyes is got blood, adopt Advantage electronic induction blood glucose meter and supporting paper slip (Switzerland Roche company product) to measure blood glucose, blood glucose value is defined as the diabetes model Mus greater than 16.7 mmol/L persons.The diabetes model Mus is divided into 7 groups at random by after the blood glucose value numbering, 3 treatment groups of composition of plant extracts (10,25,50 mg/kg), 2 groups of FTZ (250,500 mg/kg) of control carbohydrate metabolism disturbance, quenches one's thirst and pacify capsule for treating group (500 mg/kg), model control group.Each group difference gastric infusion 1 time/day, successive administration 30 days, play the animal fasting in preceding 12 hours in the last administration, got blood from mice socket of the eye venous plexus respectively in 2 hours after the administration, centrifugal 10 min of blood sample 3000 rpm, separation of serum, measure blood sugar content by the glucose kit description, experimental result sees Table 5, and NS represents normal saline in the table 5, A represents to prevent and treat the composition of plant extracts of carbohydrate metabolism disturbance, and STZ represents streptozotocin.
The composition of plant extracts of table 5 control carbohydrate metabolism disturbance is to the influence of the blood glucose of streptozotocin (STZ) type diabetic mice (n=10, x ± s)
Annotate: compare with normal group,
*P＜0.05,
*P＜0.01; Compare with model group,
△P＜0.05,
△ △P＜0.01; With comparing before the treatment
▲P＜0.05,
▲ ▲P＜0.01.
(2) influence of the blood glucose of the hyperglycemia mice that epinephrine is caused
With 60 healthy NIH mices, after weighing, numbering is divided into 6 groups at random, every group 10, get wherein that 1 group of composition of plant extracts as normal control group, epinephrine group, control carbohydrate metabolism disturbance is little, heavy dose of (25,50 mg/kg) group, FTZ 250 mg/kg group, the peace of quenching one's thirst capsule for treating group (500 mg/kg).Each group difference gastric infusion 1 time/day, normal control group and epinephrine group give the equal-volume normal saline, successive administration 7 days, behind last administration 2 h, matched group lumbar injection (ip) equal-volume normal saline, all the other respectively organize ip epinephrine (240 mg/kg) solution, 0.5 h, 1 h get blood from mice socket of the eye venous plexus behind the ip epinephrine respectively, separation of serum, mensuration blood glucose, experimental result sees Table 6, A represents to prevent and treat the composition of plant extracts of carbohydrate metabolism disturbance in the table 6, and NS represents normal saline.
The influence of the blood glucose of the hyperglycemia mice that the composition of plant extracts of table 6 control carbohydrate metabolism disturbance causes epinephrine (n=10, x ± s)
Annotate: compare with normal group,
*P＜0.05,
*P＜0.01; With comparing before the treatment
▲P＜0.05,
▲ ▲P＜0.01.
(3) influence of the blood glucose of the hyperglycemia mice that glucose is caused
With 60 healthy NIH mices, after weighing, numbering is divided into 6 groups at random, every group 10, the composition of plant extracts of normal control group, the high sugared model group of glucose, control carbohydrate metabolism disturbance is little, heavy dose of (25,50 mg/kg) organizes, FTZ 250 mg/kg organize, the peace of quenching one's thirst capsule for treating group (500 mg/kg).Each group difference gastric infusion 1 time/day, normal control group and glucose group give the equal-volume normal saline, successive administration 7 days, behind last administration 2 h, matched group lumbar injection (ip) equal-volume normal saline, all the other respectively organize ip glucose (2 g/kg) solution, 0.5,1,2 h get blood from mice socket of the eye venous plexus behind the ip glucose respectively, separation of serum, mensuration blood glucose, and experimental result sees Table 7, A represents to prevent and treat the composition of plant extracts of carbohydrate metabolism disturbance, and NS represents normal saline.
The influence of the blood glucose of the hyperglycemia mice that the composition of plant extracts of table 7 control carbohydrate metabolism disturbance causes glucose (n=10, x ± s)
Annotate: compare with normal group,
*P＜0.01; With comparing before the treatment
▲P＜0.05,
▲ ▲P＜0.01.
The composition of plant extracts of embodiment 4 control carbohydrate metabolism disturbances brings out the influence of the plain resistance of mouse islets to hydrocortisone
Be divided into 4 groups at random after 40 mices numbering weighed, every group 10, get wherein one group of subcutaneous injection normal saline as normal control group, one group of subcutaneous injection hydrocortisone (36 mg/kg), two groups of subcutaneous injection hydrocortisone (36 mg/kg) are irritated the composition of plant extracts little, heavy dose of (10,20 mg/kg) of stomach control carbohydrate metabolism disturbance more respectively in addition.Each group difference gastric infusion 1 time/day, normal control group and hydrocortisone group give the equal-volume normal saline, successive administration 10 days, behind last administration 2 h, matched group ip equal-volume normal saline, all the other respectively organize ip insulin (0.5 g/kg), and 0.5,2 h pluck eye and get blood behind the ip glucose respectively, and separation of serum, automatic clinical chemistry analyzer are measured blood glucose.Experimental result is shown in table 8, table 9, and wherein A represents to prevent and treat the composition of plant extracts of carbohydrate metabolism disturbance, and NS represents normal saline.。
The composition of plant extracts of table 8 control carbohydrate metabolism disturbance brings out influence (n=10, the x ± s) of the plain resistance of mouse islets to hydrocortisone
The composition of plant extracts of table 9 control carbohydrate metabolism disturbance brings out influence (n=10, the x ± s) of the plain resistance of mouse islets to hydrocortisone
Annotate: compare with normal group,
*P＜0.01; With comparing before the treatment
▲P＜0.05,
▲ ▲P＜0.01.
The composition of plant extracts of embodiment 5 control carbohydrate metabolism disturbances is to the effect of streptozotocin type diabetes model rat
Medicine and reagent: streptozotocin (STZ), U.S. Sigma company product; Sodium citrate, Shantou City's brilliance laboratory product, lot number 20000116.
Experimental subject is 36 of female Wistar regular grade rats, and body weight 220～250 g(No.1 Military Medical Univ. Experimental Animal Center provide).
After all rat adaptabilities are fed a week, fasting 12 h, getting 7 rats is normal control group (abbreviation matched group), other rat disposable celiacs are injected 50 mg/kg STZ, and (STZ faces the sodium citrate buffer preparation with preceding usefulness 0.1 mol/L, pH 4.5, use up in 10 min), matched group is then injected the equivalent liquor sodii citratis.Fasting glucose behind 72 h (FBG) is a hyperglycemia diabetes model rat greater than 16.7 mmol/L persons.Diabetes rat is divided into the large and small dosage group of composition of plant extracts of diabetic model group, control carbohydrate metabolism disturbance at random; Heavy dose of group by people and rat dose,equivalent than mg/5 ml/ kg administration every days 40, small dose group mg/5 ml/kg administration every days 20, model group and normal group are given 5 ml/kg distilled water every day, irritate stomach the morning once, treat 15 days.Before each organizes modeling, after the modeling, the docking of treatment back gets blood and surveys fasting glucose (FBG), serum insulin (Ins), get the rat pancreas at last and carry out HE dyeing and immunohistochemical staining as specimen.
Adopt Advantage electronic induction blood glucose meter and supporting paper slip (Switzerland Roche company product) to measure blood glucose.Survey the blood glucose glucose oxidase method, survey serum insulin with putting the method for exempting from.
(x ± s) expression carries out significance test with variance analysis method to experimental data with means standard deviation.
Influence to blood glucose the results are shown in Table 10, wherein prevents and treats the composition of plant extracts of carbohydrate metabolism disturbance and represents with A.Model group and normal group compare, and FBG significantly raises (P＜0.01), significantly descend (P＜0.01) with model group comparison FBG after the big or small dosage treatment, illustrate that the composition of plant extracts of control carbohydrate metabolism disturbance has remarkable hypoglycemic activity.
The composition of plant extracts of table 10 control carbohydrate metabolism disturbance is respectively organized rat blood sugar change list (mmol/L) to the STZ model
| Group | n | Dosage (mg/kg) | Before the modeling | Before treating after the modeling | After the treatment |
| Normal group | 7 | / | 5.56±0.53 | 5.61±0.52 | 5.67±0.67 |
| Model group | 7 | / | 5.61±0.56 | 20.28±2.63 ** | 22.86±2.81 * |
| The A small dose group | 7 | 20 | 5.63±0.68 | 20.91±2.48 ** | 15.40±2.16 **△△▲▲ |
| The heavy dose of group of A | 7 | 40 | 5.59±0.71 | 21.23±2.61 ** | 12.51±3.32 **△△▲▲ |
| FTZ | 7 | 500 | 5.68±0.64 | 20.71±2.34 ** | 16.71±2.31 **△▲▲ |
Annotate: compare with normal group,
*P＜0.05,
*P＜0.01; Compare with model group,
△P＜0.05,
△ △P＜0.01; With comparing before the treatment
▲P＜0.05,
▲ ▲P＜0.01.
Influence to serum insulin the results are shown in Table 11, wherein prevents and treats the composition of plant extracts of carbohydrate metabolism disturbance and represents with A.Model group and normal group compare, and Ins significantly reduces (P＜0.01), and serum Ins level shows that than the remarkable rising of model group (P＜0.05) secretion has certain facilitation to the composition of plant extracts of preventing and treating carbohydrate metabolism disturbance to Ins after the big or small dosage treatment.
The composition of plant extracts of table 11 control carbohydrate metabolism disturbance is respectively organized rat blood serum insulin change list (mU/L) to the STZ model
| Group | n | Dosage (mg/kg) | Before the modeling | Before treating after the modeling | After the treatment |
| Normal group | 7 | / | 29.25±2.37 | 29.16±2.48 | 29.32±2.31 |
| Model group | 7 | / | 29.17±2.81 | 19.01±2.79 ** | 16.14±3.61 ** |
| The A small dose group | 7 | 20 | 29.21±3.58 | 19.36±4.01 ** | 21.23±3.71 * |
| The heavy dose of group of A | 7 | 40 | 28.95±3.31 | 18.90±3.41 ** | 23.11±3.82 * |
| FTZ | 7 | 500 | 29.06±2.69 | 18.31±2.82 ** | 19.96±2.45 ** |
Annotate: compare with normal group,
*P＜0.05,
*P＜0.01.
HE dyeing histological observation is found: the normal group islets of langerhans is circular, elliptical erythrocyte group, and boundary is clear, and islets of langerhans number and island inner cell number are more, and kytoplasm is abundant; STZ model group islets of langerhans number and island inner cell number reduce, and cellular morphology is irregular, and nuclear differs in size, form differs, the part karyopycnosis, and a few cell is the cavity shape; Heavy dose of group islets of langerhans number and island inner cell are counted showed increased, and cell distribution is even, and the nuclear size is equal substantially, no karyopycnosis; Little dose of group islets of langerhans number and island inner cell number increase, and form is rule comparatively, the small part karyopycnosis.The composition of plant extracts of this explanation control carbohydrate metabolism disturbance has significant protective effect to the islet cells tissue of STZ MODEL DAMAGE, and can promote secretion of insulin.
The composition of plant extracts of embodiment 6 control carbohydrate metabolism disturbances is to the influence of B cell
(1) composition of plant extracts of control carbohydrate metabolism disturbance is to the influence of B cell SOD, CAT and GSH-Px activity and GSH content
Cultivate rat Langerhans islet B cell routinely, treat to add 200 μ M H respectively behind the cell fusion
2O
2Educate 1 h altogether, the composition of plant extracts and the FTZ that add the control carbohydrate metabolism disturbance of various variable concentrations then, after continuing to cultivate 24 h, collecting cell is made cell homogenates, with oxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) and glutathion (GSH) detection kit, adopt colorimetry to detect SOD, CAT and GSH-Px activity and GSH content, the composition of plant extracts of studying above-mentioned variable concentrations control carbohydrate metabolism disturbance is to B cell SOD, CAT and GSH-Px activity and the active influence of GSH content.Data are represented with means standard deviation, carry out significance test with variance analysis method.
Experimental result shows, the composition of plant extracts of various dose control carbohydrate metabolism disturbance and FTZ handle back B cell SOD, CAT and GSH-Px activity and GSH content significantly increases (P＜0.01), and the enhancing of obvious dose dependent is arranged, the results are shown in Table 12, BBR represents berberine hydrochloride in the table, plays the contrast effect; A represents to prevent and treat the composition of plant extracts of carbohydrate metabolism disturbance.The composition of plant extracts that control carbohydrate metabolism disturbance of the present invention is described has the effect of significantly improving to B cell SOD, CAT and GSH-Px activity and GSH content, helps antioxidant stress injury.The protection B cell.
Table 12 different pharmaceutical is to the influence of oxidative stress damage B cell SOD, CAT and GSH-Px activity and GSH content
Annotate: compare * P＜0.05, * * P＜0.01 with the normal control group; Compare with normal group,
☆ ☆P＜0.01.
(2) composition of plant extracts of control carbohydrate metabolism disturbance is to the influence of Bcl-2, PPAR-a and Bax in the B cell and Fas gene expression
It is identical with (1) to handle the B cell method, extract total RNA in the B cell with Trizol reagent, the RT-PCR method is measured Bcl-2 and Bax and Fas expression conditions in the B cell, observes the influence of the composition of plant extracts of control carbohydrate metabolism disturbance to Bcl-2, PPAR-a and Bax in the B cell and Fas gene expression.Data are represented with means standard deviation, carry out significance test with variance analysis method.
Experimental result shows; Bcl-2 and Bax and Fas gene expression are expressed normal in the normal group B cell; Bcl-2 gene expression significantly increases (P＜0.01) in the composition of plant extracts processing back B cell of various dose control carbohydrate metabolism disturbance; and Bax and Fas gene expression significantly reduce (P＜0.01); illustrate and the present invention prevent and treat the composition of plant extracts of carbohydrate metabolism disturbance gene expression has obvious facilitation (P＜0.01) to Bcl-2; and the expression of inhibition apoptosis gene Bax and Fas, thereby the protection B cell is avoided oxidative stress damage apoptosis.The results are shown in Table 13, BBR represents berberine hydrochloride in the table, and A represents to prevent and treat the composition of plant extracts of carbohydrate metabolism disturbance.
Table 13 different pharmaceutical is to the influence of Bcl-2, PPAR-a and Bax in the B cell and Fas gene expression
Annotate: compare with model group,
*P＜0.05,
*P＜0.01; Compare with normal group,
☆ ☆P＜0.01.
Conclusion: the composition of plant extracts of control carbohydrate metabolism disturbance is to confirm that through pharmacological research tool promotes insulin secretion, and the content that increases serum insulin has blood sugar lowering, improves the metabolic effect of blood glucose.Its mechanism of action may comprise:
(1) composition of plant extracts of control carbohydrate metabolism disturbance can strengthen the active and GSH content of B cellular oxidation thing dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px), can suppress lipid peroxidation and remove hydroxyl radical free radical, antioxidant stress injury promotes B cell regeneration.
(2) composition of plant extracts of control carbohydrate metabolism disturbance can increase the cl-2 gene expression of B cell anti-apoptotic factor B and suppress B cell antiapoptotic factors Bax and Fas gene expression, thereby reduce the generation of apoptosis, stimulate normal B cell excreting insulin.
(3) composition of plant extracts of control carbohydrate metabolism disturbance can increase B cell anti-inflammatory factor PPAR-a gene expression, and the antagonism role of cytokines reduces the B cell damage.Increase the content of serum insulin by protection, reparation B cell.
Clinical research is the result show, the composition of plant extracts of composition of plant extracts control carbohydrate metabolism disturbance of the present invention has significant blood sugar lowering, improves the blood glucose metabolism, can be used for treating carbohydrate metabolism disturbance relevant diseases such as hyperglycemia and diabetes.
Claims (10)
1. a composition of plant extracts of preventing and treating carbohydrate metabolism disturbance comprises extract of panax notoginseng saponins and Rhizoma Coptidis total alkaloids extract.
2. the composition of plant extracts of control carbohydrate metabolism disturbance according to claim 1, the weight ratio that it is characterized in that described extract of panax notoginseng saponins and Rhizoma Coptidis total alkaloids extract is 1 ~ 9:1 ~ 3.
3. the preparation method of the composition of plant extracts of claim 1 or 2 described control carbohydrate metabolism disturbances, step is as follows:
(1) preparation of extract of panax notoginseng saponins: with material medicine Radix Notoginseng process C
1-3After the alcohol extraction, merge total extract, obtain concentrated solution behind the concentrated total extract, remove non-saponin component in the concentrated solution, obtain extract of panax notoginseng saponins;
(2) preparation of Rhizoma Coptidis total alkaloids extract: the material medicine Rhizoma Coptidis is added C
1-3Alcohol reflux extracts, and reclaims C
1-3Alcohol, concentrate concentrated solution, separate out the Rhizoma Coptidis total alkaloids extract with acid dissolving alkali precipitation recrystallization again;
(3) extract of panax notoginseng saponins is mixed with the Rhizoma Coptidis total alkaloids extract, obtain preventing and treating the composition of plant extracts of carbohydrate metabolism disturbance.
4. according to the preparation method of the composition of plant extracts of the described control carbohydrate metabolism disturbance of claim 3, it is characterized in that step (1) C
1-3Alcohol extraction is the C with 30～95 volume %
1-3Alcohol extraction 1～5 time, each C that extracts
1-3The alcohol volume is 1～15 times of quality of medicinal material, and each extraction time is more than 5 minutes; Described C
1-3Alcohol is methanol, ethanol or propanol.
5. according to the preparation method of the composition of plant extracts of the described control carbohydrate metabolism disturbance of claim 3, it is characterized in that removing in the step (1) non-saponin component employing macroporous resin in the concentrated solution, the amount ratio of macroporous resin and concentrated solution is that the 1kg macroporous resin adds concentrated solution 1 ~ 5L.
6. according to the preparation method of the composition of plant extracts of the described control carbohydrate metabolism disturbance of claim 5, it is characterized in that it being with 50 ~ 95 volume % ethanol with macroporous resin column absorption back eluting, consumption be every 1kg macroporous resin with 5 ~ 20 L ethanol, with 1 ~ 20 ml/min flow velocity eluting, be eluted to colourless; Described macroporous resin is polyamides amine type resin or polystyrene weak-base anion-exchange resin.
7. according to the preparation method of the composition of plant extracts of the described control carbohydrate metabolism disturbance of claim 3, it is characterized in that step (2) C
1-3It is with 50 ~ 70 volume %C that alcohol reflux extracts
1-3Alcohol extraction 1～5 time, each C that extracts
1-3The alcohol volume is 1～15 times of quality of medicinal material, and each extraction time is more than 5 minutes.
8. according to the preparation method of the composition of plant extracts of the described control carbohydrate metabolism disturbance of claim 3, it is characterized in that the described acid dissolving of step (2) is use acetate dissolution, pH is 0.1 ~ 5 with 1 ~ 30 quality %HCl accent.
9. according to the preparation method of the composition of plant extracts of the described control carbohydrate metabolism disturbance of claim 3, it is characterized in that the described alkali precipitation of step (2) is that pH is 9 ~ 13 with 1 ~ 30 quality %NaOH solution, the recrystallization volume is 1 ~ 5 times of Rhizoma Coptidis weight.
The composition of plant extracts of claim 1 or 2 described control carbohydrate metabolism disturbances preparation prevention or treatment carbohydrate metabolism disturbance disease medicament and or health product in application.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100267856A CN102100760A (en) | 2011-01-25 | 2011-01-25 | Plant extract composition capable of preventing and treating sugar metabolism disturbance and preparation method thereof |
| PCT/CN2011/071011 WO2012100441A1 (en) | 2011-01-25 | 2011-02-16 | Plant extract composition used for preventing and treating carbohydrate metabolism disorder and preparative method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100267856A CN102100760A (en) | 2011-01-25 | 2011-01-25 | Plant extract composition capable of preventing and treating sugar metabolism disturbance and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102100760A true CN102100760A (en) | 2011-06-22 |
Family
ID=44153960
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100267856A Pending CN102100760A (en) | 2011-01-25 | 2011-01-25 | Plant extract composition capable of preventing and treating sugar metabolism disturbance and preparation method thereof |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN102100760A (en) |
| WO (1) | WO2012100441A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012100442A1 (en) * | 2011-01-25 | 2012-08-02 | 广州中医药大学 | Plant extract composition used for preventing and treating arteriosclerosis and preparative method thereof |
| WO2012100440A1 (en) * | 2011-01-25 | 2012-08-02 | 广州中医药大学 | Plant extract composition used for preventing and treating lipid metabolism disorder and preparative method thereof |
| CN107860832A (en) * | 2017-10-19 | 2018-03-30 | 遵义医学院 | The method for building up of compound rubarb Qi Yi Tang finger-print |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101396445A (en) * | 2008-10-31 | 2009-04-01 | 中山大学 | Use of Chinese goldthread total alkali in preparing medicine for treating diabetes complication |
| CN101700264A (en) * | 2009-11-20 | 2010-05-05 | 许淑清 | Application of pseudo-ginseng and extract thereof in preparing medicament for curing and/or preventing diabetic neuropathies |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102100761B (en) * | 2011-01-25 | 2013-11-13 | 广州中医药大学 | Plant extract composition for preventing and treating lipidmetabolic disorder and preparation method thereof |
| CN102160876B (en) * | 2011-01-25 | 2012-06-13 | 广州中医药大学 | Plant extract composition for preventing and treating arteriosclerosis and preparation method thereof |
-
2011
- 2011-01-25 CN CN2011100267856A patent/CN102100760A/en active Pending
- 2011-02-16 WO PCT/CN2011/071011 patent/WO2012100441A1/en active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101396445A (en) * | 2008-10-31 | 2009-04-01 | 中山大学 | Use of Chinese goldthread total alkali in preparing medicine for treating diabetes complication |
| CN101700264A (en) * | 2009-11-20 | 2010-05-05 | 许淑清 | Application of pseudo-ginseng and extract thereof in preparing medicament for curing and/or preventing diabetic neuropathies |
Non-Patent Citations (1)
| Title |
|---|
| 《中成药》 20070630 杨红艳等 均匀设计优选黄连、三七提取工艺条件 891-893 1-10 第29卷, 第6期 2 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012100442A1 (en) * | 2011-01-25 | 2012-08-02 | 广州中医药大学 | Plant extract composition used for preventing and treating arteriosclerosis and preparative method thereof |
| WO2012100440A1 (en) * | 2011-01-25 | 2012-08-02 | 广州中医药大学 | Plant extract composition used for preventing and treating lipid metabolism disorder and preparative method thereof |
| CN107860832A (en) * | 2017-10-19 | 2018-03-30 | 遵义医学院 | The method for building up of compound rubarb Qi Yi Tang finger-print |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012100441A1 (en) | 2012-08-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102133221B (en) | Compound traditional Chinese medicine extract preventing glucose metabolism disturbance and preparation method thereof | |
| CN102091083B (en) | Petroleum ether extract of traditional Chinese medicine for preventing and treating glucose and lipid metabolic disturbance and preparation method thereof | |
| CN103536635A (en) | Preparation method of holothuria nobilis and application thereof in treatment of diabetes mellitus | |
| CN101695520B (en) | Preparation method of medicament for treating diabetes | |
| CN115364170B (en) | Sugar metabolism regulator and preparation method and application thereof | |
| CN101816723B (en) | Sobering up and liver protecting Chinese medicinal preparation and preparation method thereof | |
| CN102743401A (en) | Application of panaxadiol saponins fraction in preparing medicine for preventing epilepsia | |
| CN102100760A (en) | Plant extract composition capable of preventing and treating sugar metabolism disturbance and preparation method thereof | |
| CN102100761B (en) | Plant extract composition for preventing and treating lipidmetabolic disorder and preparation method thereof | |
| CN103585192B (en) | A kind of preparation method of Aleuritopteris argentea (Gmel.) Fee extract and application thereof | |
| CN113730464A (en) | New application of rhizoma coptidis pill, extract and pharmaceutical composition thereof and rhizoma coptidis pill product | |
| CN101953887B (en) | Medicinal composition for preventing and treating diabetic complications and preparation method thereof | |
| CN113730463A (en) | New application of xianglian pill product | |
| CN103432420B (en) | A kind of Chinese medicine composition for the treatment of diabetes and preparation method thereof and detection method | |
| CN102357131A (en) | Method for preparing astragalus polysaccharide glucose-lowering capsule | |
| CN102836349B (en) | Traditional Chinese medicine composition for treating diabetes and preparation method thereof | |
| CN104547588B (en) | A kind of preparation method of stomach health drop pills | |
| CN103877323B (en) | Medicinal composition for treating non-alcoholic fatty liver disease | |
| CN100355440C (en) | Compound Chinese medicinal preparation for treating type II diabetes and lowering blood sugar and its preparation method | |
| CN101874830B (en) | New application of golden thread processed product | |
| CN101249129B (en) | Chinese medicine extract combination and medicine use thereof | |
| CN115252729B (en) | Traditional Chinese medicine composition for treating diabetes and application thereof | |
| CN105395654A (en) | Traditional Chinese medicine composition for preventing and curing glucose metabolic disorder and preparation method thereof | |
| CN101926848B (en) | Medicinal composition for treating heart cerebrovascular diseases and preparation thereof | |
| CN102028725B (en) | Traditional Chinese medicine composition, extract, preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20110622 |