CN102282465A - 用于检测二醇的设备和方法 - Google Patents
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
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- G—PHYSICS
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- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
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Abstract
Description
wt%DEG | mM DEG | DEG(μl) | 牙膏(μl) | 总的缓冲液(ml) |
100 | 569 | 135 | 0 | 2.365 |
80 | 455 | 108 | 180 | 2.212 |
60 | 341 | 81 | 360 | 2.059 |
40 | 228 | 54 | 540 | 1.825 |
30 | 171 | 40.5 | 630 | 1.830 |
20 | 114 | 27 | 720 | 1.753 |
10 | 57 | 13.5 | 810 | 1.680 |
5 | 28 | 6.7 | 849 | 1.650 |
3 | 17 | 4.05 | 873 | 1.623 |
1 | 6 | 1.35 | 891 | 1.608 |
0 | 0 | 0 | 900 | 1.600 |
wt%DEG | mM DEG | DEG(μl) | 试样(μl) | 总的缓冲液(ml) |
100 | 569 | 135 | 0 | 2.365 |
80 | 455 | 108 | 27 | 2.365 |
60 | 341 | 81 | 54 | 2.365 |
40 | 228 | 54 | 81 | 2.365 |
30 | 171 | 40.5 | 94.5 | 2.365 |
20 | 114 | 27 | 108 | 2.365 |
10 | 57 | 13.5 | 121.5 | 2.365 |
5 | 28 | 6.7 | 127.3 | 2.365 |
3 | 17 | 4.05 | 130.95 | 2.365 |
1 | 6 | 1.35 | 133.65 | 2.365 |
0 | 0 | 0 | 135 | 2.365 |
Claims (21)
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US19928908P | 2008-11-14 | 2008-11-14 | |
US61/199,289 | 2008-11-14 | ||
PCT/US2009/064402 WO2010057005A2 (en) | 2008-11-14 | 2009-11-13 | Apparatus and method for detecting glycol |
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CN102282465A true CN102282465A (zh) | 2011-12-14 |
CN102282465B CN102282465B (zh) | 2014-04-16 |
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CN103376247A (zh) * | 2012-04-26 | 2013-10-30 | 北京和信非凡生物技术有限公司 | 一种琥珀酸-q还原酶酶活性检测方法和试剂 |
CN111487403A (zh) * | 2019-01-28 | 2020-08-04 | 西北农林科技大学 | 一种便携式的酶联免疫样品液吸光度检测仪器及检测方法 |
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WO2010057005A2 (en) | 2008-11-14 | 2010-05-20 | Melanie Margarete Hoehl | Apparatus and method for detecting glycol |
CN101581661B (zh) * | 2009-06-25 | 2012-06-20 | 长春吉大·小天鹅仪器有限公司 | 多参数集成式比色池 |
CN102517378A (zh) * | 2011-11-16 | 2012-06-27 | 浙江工业大学 | 一种基于nad(p)h荧光的醇脱氢酶的筛选鉴定方法 |
US9678029B2 (en) | 2014-08-22 | 2017-06-13 | Honeywell International Inc. | Oxidation catalyst detector for aircraft components |
US11828696B2 (en) * | 2020-12-16 | 2023-11-28 | Caterpillar Inc. | System and method for processing data from a particle monitoring sensor |
Family Cites Families (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3918812A (en) * | 1973-05-07 | 1975-11-11 | Us Energy | Diagnoses of disease states by fluorescent measurements utilizing scanning laser beams |
DE2408646A1 (de) * | 1974-02-22 | 1975-08-28 | Max Planck Gesellschaft | Reaktionskinetisches messgeraet |
US4324558A (en) * | 1979-08-02 | 1982-04-13 | Obermayer Arthur S | Method of monitoring toxic fluids |
US4573796A (en) * | 1984-01-06 | 1986-03-04 | The United States Of America As Represented By The United States Department Of Energy | Apparatus for eliminating background interference in fluorescence measurements |
JPS63230082A (ja) * | 1987-03-19 | 1988-09-26 | Fujirebio Inc | アルコ−ルデヒドロゲナ−ゼ |
SE8802536D0 (sv) * | 1988-07-07 | 1988-07-07 | Altoptronic Ab | Metod och apparat for spektroskopisk metning av koncentrationen av en gas i ett prov |
US5252834A (en) * | 1990-11-13 | 1993-10-12 | Union Oil Company Of California | Pulsed and gated multi-mode microspectrophotometry device and method |
SE501428C2 (sv) * | 1993-06-24 | 1995-02-13 | Cma Microdialysis Res Ab | Fluorescensdetektor jämte bäranordning för utbytbar provkuvett vid en fluorescensdetektor |
US5930000A (en) * | 1998-02-11 | 1999-07-27 | Monitor Labs, Inc. | Line-locked diode laser for gas spectroscopy |
US6853452B1 (en) * | 1999-03-17 | 2005-02-08 | University Of Virginia Patent Foundation | Passive remote sensor of chemicals |
EP1309719B2 (en) * | 2000-08-14 | 2022-09-07 | University of Maryland at Baltimore County | Bioreactor and bioprocessing technique |
US6870165B2 (en) * | 2001-10-19 | 2005-03-22 | Biocal Technology, Inc. | Multi-color multiplexed analysis in a bio-separation system |
US7190450B2 (en) * | 2003-02-20 | 2007-03-13 | Yale University | Systems and methods for sorting aerosols |
US7106442B2 (en) * | 2003-04-29 | 2006-09-12 | Silcott David B | Multi-spectral optical method and system for detecting and classifying biological and non-biological particles |
US20070196927A1 (en) * | 2003-09-04 | 2007-08-23 | Christian Grandfils | Method For Qualitative And/Or Quantitative Detection Of Polyethylene Glycols In Biological Fluids |
US7339668B2 (en) * | 2005-10-18 | 2008-03-04 | Thermo Electron Scientific Instruments Llc | Spectrometer for analysis of multiple samples |
CN1866020A (zh) * | 2006-06-08 | 2006-11-22 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | 同时检测壬基酚、阿特拉津和雌二醇的试剂盒及制备方法 |
JP4417353B2 (ja) * | 2006-07-13 | 2010-02-17 | 麒麟麦酒株式会社 | 発酵麦芽飲料またはビール様飲料中に混入したプロピレングリコールの検出方法 |
SG178773A1 (en) * | 2006-11-13 | 2012-03-29 | Ateris Technologies Llc | Pesticide biomarker |
US20080174768A1 (en) * | 2007-01-18 | 2008-07-24 | Mathias Belz | Self referencing LED detection system for spectroscopy applications |
US7884937B2 (en) * | 2007-04-19 | 2011-02-08 | Science & Engineering Services, Inc. | Airborne tunable mid-IR laser gas-correlation sensor |
WO2010057005A2 (en) | 2008-11-14 | 2010-05-20 | Melanie Margarete Hoehl | Apparatus and method for detecting glycol |
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2009
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Cited By (2)
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CN103376247A (zh) * | 2012-04-26 | 2013-10-30 | 北京和信非凡生物技术有限公司 | 一种琥珀酸-q还原酶酶活性检测方法和试剂 |
CN111487403A (zh) * | 2019-01-28 | 2020-08-04 | 西北农林科技大学 | 一种便携式的酶联免疫样品液吸光度检测仪器及检测方法 |
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US20100124758A1 (en) | 2010-05-20 |
WO2010057005A2 (en) | 2010-05-20 |
CN102282465B (zh) | 2014-04-16 |
US8867037B2 (en) | 2014-10-21 |
WO2010057005A3 (en) | 2010-08-26 |
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