CN102266410A - Extraction method of effective components of Morinda citrifolia leaves - Google Patents
Extraction method of effective components of Morinda citrifolia leaves Download PDFInfo
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- CN102266410A CN102266410A CN2011102648369A CN201110264836A CN102266410A CN 102266410 A CN102266410 A CN 102266410A CN 2011102648369 A CN2011102648369 A CN 2011102648369A CN 201110264836 A CN201110264836 A CN 201110264836A CN 102266410 A CN102266410 A CN 102266410A
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Abstract
The invention relates to an extraction method of effective components of Morinda citrifolia leaves, and the extraction method comprises the following concrete steps: crushing Morinda citrifolia leaves, performing multi-tank dynamic countercurrent extraction, filtering extractive solution, performing rotary concentration, adding water to concentrated solution to a certain volume, filtering, separating filtrate with macroporous resin, firstly eluting with water, then eluting with ethanol solution, concentrating ethanol eluate, spray-drying supernatant, drying precipitates under reduced pressure, and mixing to obtain an extract. The extraction method provided by the invention has high extraction yield and is easy to realize industrial scale-up.
Description
Technical field
The extracting method of a kind of active components in medicinal plant of the present invention is specifically related to a kind of Morinda Citifolia leaf extraction of effective components.
Background technology
Morinda Citifolia (
Morinda citrifoliaL.) have another name called Morinda citrifolia Linn., Folium Citri tangerinae crust halberd, be commonly called as noni, Saurauia tristyla DC. var. oldhamii (Hemsl.) Finet ﹠ Gagncp., be Rubiaceae Radix Morindae Officinalis platymiscium, its leaf, root, skin and fruit all can be treated disease.Morinda Citifolia has bimillennial history among the people as health care and medicinal drink, particularly in south, the Pacific Ocean island, for example the Pohle Buddhist nun Asia of Tahiti, Malaysia, among the Australia and the Hawaiian indigenous people, the Morinda Citifolia daily health products that is absolutely necessary.The synthesis result of clinical pharmacology research shows that Morinda Citifolia can be safeguarded the normal function of human cell tissue, strengthens body immunity, change bacterioflora ecology and improve gastral function, regulate physiological function, help for sleep and alleviate stress muscle power and endurance, fat-reducing and skin maintenance.Aspect medical, its energy antiviral, anticancer (as pulmonary carcinoma) is to treatment autoimmune disease (as rheumatism), stop the generation of acute and chronic pain, to respiratory tract diseases such as asthma, diabetes, nephritis, arthritis, hysteria, Sensitive disease, arteriosclerosis, menoxenia, cardiovascular disease (hypertension, myocardial infarction) all has curative effect.One band have the laudatory title of " celestial fruit " in the South Pacific Ocean, is described as " the Nature is bestowed the outstanding treasure to the mankind ".
The Morinda Citifolia leaf to gingivitis, periodontal disease, laryngalgia, laryngitis, flu, headache, fracture, sprain, dermatosis etc. has certain effect.The chemical constitution study report of relevant Morinda Citifolia leaf is few, the preliminary qualitative examination of rarely seen its chemical constituent etc.At present, result of study shows that the Morinda Citifolia leaf contains flavone, saponin, iridoid glycoside, Ingredients Such As Coumarin, wherein flavone compound is one of its main component, has antioxidation, removes multiple biological activitys such as free radical, defying age, absorption ultraviolet and radioprotective.
Research for the Morinda Citifolia leaf still is in the starting stage, does not see the patent report of associated extraction technology.
Summary of the invention
The purpose of this invention is to provide Morinda Citifolia leaf extraction of effective components.
Morinda Citifolia leaf effective component extracts of the present invention, through assay, wherein total flavones 38-46%, total saponins 8-14% and total iridoid glycoside 26-30%, three's sum is 72-90%.Assay method belongs to existing routine techniques.
Its technical scheme is:
Morinda Citifolia leaf extraction of effective components, wherein the total flavones of Morinda Citifolia leaf effective component extracts, total saponins and total iridoid glycoside content sum are 72-90%, it is characterized in that concrete steps comprise:
(1) extract: the Morinda Citifolia leaf is pulverized, and is solvent with the alcoholic solution of 60-70%, carries out the multipot type dynamic countercurrent extraction, obtains extracting solution after the filtration;
(2) remove impurity: the rotation of said extracted liquid is concentrated, add water to certain volume, filter, macroporous resin on the filtrate is used the 3-5BV water elution earlier, continues with 4-6BV65% alcoholic solution eluting, collects ethanol elution;
(3) drying: it is 1.05-1.1 that eluent is concentrated into relative density, the supernatant spray drying, and the precipitation drying under reduced pressure, mix homogeneously obtains extract.
The described multipot type dynamic countercurrent extraction of step (1) is to be a cycling element with the 3-5 jar, and every jar is extracted 3 times, and every jar of second time, extracting solution is respectively as next jar first time, the solvent that extracts for the second time for the third time, every jar for the first time extracting solution put into fluid reservoir.
The optional AB-8 of the described macroporous resin of step (2), D101 or HPD-400 type.
The invention has the beneficial effects as follows:
1, the present invention has changed traditional production technology, adopts multi-stage counter current extraction, has realized the counter current contacting of solvent and material liquid, Concentraton gradient is big, and it is big to extract motive force, and avoids the troublesome poeration that uses separatory funnel repeatedly to extract, the extraction efficiency height is beneficial to big production operation;
2, macroporous resin column applied sample amount of the present invention is big, with short production cycle, and separating degree is good, the product purity height.
The specific embodiment
Content of the present invention is further elaborated by following embodiment, but does not limit the scope of the invention.
Embodiment 1:
The Morinda Citifolia leaf is pulverized, got 2kg, the alcoholic solution with 60% is a solvent, carry out the multipot type dynamic countercurrent extraction, with 3 jars be a cycling element, every jar is extracted every jar of second time 3 times, extracting solution is respectively as next jar first time for the third time, the solvent that extract the second time, every jar for the first time extracting solution put into fluid reservoir, obtain extracting solution after the filtration, rotation concentrates, and adds water to certain volume, filter, HPD-400 type macroporous resin on the filtrate is used the 5BV water elution earlier, continues with 5BV65% alcoholic solution eluting, collect ethanol elution, being concentrated into relative density is 1.05, supernatant spray drying, precipitation drying under reduced pressure, mix homogeneously obtains extract 33g.
Embodiment 2:
The Morinda Citifolia leaf is pulverized, got 2kg, the alcoholic solution with 65% is a solvent, carry out the multipot type dynamic countercurrent extraction, with 3 jars be a cycling element, every jar is extracted every jar of second time 3 times, extracting solution is respectively as next jar first time for the third time, the solvent that extract the second time, every jar for the first time extracting solution put into fluid reservoir, obtain extracting solution after the filtration, rotation concentrates, and adds water to certain volume, filter, D101 type macroporous resin on the filtrate is used the 3BV water elution earlier, continues with 4BV65% alcoholic solution eluting, collect ethanol elution, being concentrated into relative density is 1.05, supernatant spray drying, precipitation drying under reduced pressure, mix homogeneously obtains extract 50g.
Embodiment 3:
The Morinda Citifolia leaf is pulverized, got 5kg, the alcoholic solution with 70% is a solvent, carry out the multipot type dynamic countercurrent extraction, with 4 jars be a cycling element, every jar is extracted every jar of second time 3 times, extracting solution is respectively as next jar first time for the third time, the solvent that extract the second time, every jar for the first time extracting solution put into fluid reservoir, obtain extracting solution after the filtration, rotation concentrates, and adds water to certain volume, filter, HPD-400 type macroporous resin on the filtrate is used the 3BV water elution earlier, continues with 6BV65% alcoholic solution eluting, collect ethanol elution, being concentrated into relative density is 1.1, supernatant spray drying, precipitation drying under reduced pressure, mix homogeneously obtains extract 173g.
Embodiment 4:
The Morinda Citifolia leaf is pulverized, got 10kg, the alcoholic solution with 70% is a solvent, carry out the multipot type dynamic countercurrent extraction, with 4 jars be a cycling element, every jar is extracted every jar of second time 3 times, extracting solution is respectively as next jar first time for the third time, the solvent that extract the second time, every jar for the first time extracting solution put into fluid reservoir, obtain extracting solution after the filtration, rotation concentrates, and adds water to certain volume, filter, D101 type macroporous resin on the filtrate is used the 4BV water elution earlier, continues with 6BV65% alcoholic solution eluting, collect ethanol elution, being concentrated into relative density is 1.05, supernatant spray drying, precipitation drying under reduced pressure, mix homogeneously obtains extract 352g.
Embodiment 5:
The Morinda Citifolia leaf is pulverized, got 20kg, the alcoholic solution with 60% is a solvent, carry out the multipot type dynamic countercurrent extraction, with 5 jars be a cycling element, every jar is extracted every jar of second time 3 times, extracting solution is respectively as next jar first time for the third time, the solvent that extract the second time, every jar for the first time extracting solution put into fluid reservoir, obtain extracting solution after the filtration, rotation concentrates, and adds water to certain volume, filter, AB-8 type macroporous resin on the filtrate is used the 5BV water elution earlier, continues with 6BV65% alcoholic solution eluting, collect ethanol elution, being concentrated into relative density is 1.05, supernatant spray drying, precipitation drying under reduced pressure, mix homogeneously obtains extract 669g.
Claims (3)
1. Morinda Citifolia leaf extraction of effective components, wherein the total flavones of Morinda Citifolia leaf effective component extracts, total saponins and total iridoid glycoside content sum are 72-90%, it is characterized in that concrete steps comprise:
(1) extract: the Morinda Citifolia leaf is pulverized, and is solvent with the alcoholic solution of 60-70%, carries out the multipot type dynamic countercurrent extraction, obtains extracting solution after the filtration;
(2) remove impurity: the rotation of said extracted liquid is concentrated, and concentrated solution adds water to certain volume, filters, and macroporous resin on the filtrate is used the 3-5BV water elution earlier, continues with 4-6BV65% alcoholic solution eluting, collects ethanol elution;
(3) drying: it is 1.05-1.1 that eluent is concentrated into relative density, the supernatant spray drying, and the precipitation drying under reduced pressure, mix homogeneously obtains extract.
2. Morinda Citifolia leaf extraction of effective components according to claim 1, it is characterized in that the described multipot type dynamic countercurrent extraction of step (1) is is a cycling element with the 3-5 jar, every jar is extracted 3 times, every jar of second time, extracting solution is respectively as next jar first time, the solvent that extracts for the second time for the third time, every jar for the first time extracting solution put into fluid reservoir.
3. Morinda Citifolia leaf extraction of effective components according to claim 1 is characterized in that the optional AB-8 of the described macroporous resin of step (2), D101 or HPD-400 type.
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| CN2011102648369A CN102266410A (en) | 2011-09-08 | 2011-09-08 | Extraction method of effective components of Morinda citrifolia leaves |
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| CN2011102648369A CN102266410A (en) | 2011-09-08 | 2011-09-08 | Extraction method of effective components of Morinda citrifolia leaves |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104587063A (en) * | 2015-02-06 | 2015-05-06 | 桂林双象生物科技有限公司 | Method for extracting total flavonoids from Adinandra nitida Merr.ex H.L.Li |
| CN106833978A (en) * | 2017-01-17 | 2017-06-13 | 孟蕲翾 | It is a kind of with antibacterial, acne-removal function beautiful handmade soap of promise and preparation method thereof |
| CN107998030A (en) * | 2017-12-26 | 2018-05-08 | 徐州昌南医疗科技有限公司 | A kind of skin-care makeup-removal oil formula and preparation method thereof |
| CN109745239A (en) * | 2017-11-02 | 2019-05-14 | 前海玖诚国际实业(深圳)有限公司 | A kind of formula and preparation method thereof for glue of removing ornaments and formal dress |
-
2011
- 2011-09-08 CN CN2011102648369A patent/CN102266410A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104587063A (en) * | 2015-02-06 | 2015-05-06 | 桂林双象生物科技有限公司 | Method for extracting total flavonoids from Adinandra nitida Merr.ex H.L.Li |
| CN106833978A (en) * | 2017-01-17 | 2017-06-13 | 孟蕲翾 | It is a kind of with antibacterial, acne-removal function beautiful handmade soap of promise and preparation method thereof |
| CN109745239A (en) * | 2017-11-02 | 2019-05-14 | 前海玖诚国际实业(深圳)有限公司 | A kind of formula and preparation method thereof for glue of removing ornaments and formal dress |
| CN107998030A (en) * | 2017-12-26 | 2018-05-08 | 徐州昌南医疗科技有限公司 | A kind of skin-care makeup-removal oil formula and preparation method thereof |
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Application publication date: 20111207 |