CN102258613B - Sophora alopecuroide skin bacteriostatic spray and preparation method thereof - Google Patents
Sophora alopecuroide skin bacteriostatic spray and preparation method thereof Download PDFInfo
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- CN102258613B CN102258613B CN2011101863387A CN201110186338A CN102258613B CN 102258613 B CN102258613 B CN 102258613B CN 2011101863387 A CN2011101863387 A CN 2011101863387A CN 201110186338 A CN201110186338 A CN 201110186338A CN 102258613 B CN102258613 B CN 102258613B
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Abstract
The invention provides a sophora alopecuroide skin bacteriostatic spray and a preparation method thereof. The preparation method mainly comprises the following steps of: (1) adding heartleaf houttuynia herb, szechuan lovage rhizome, rhubarb and schizonepeta spike into an extraction tank in a mixed way, and extracting by using ethanol to obtain total filtrate which is recorded as liquid A; (2) adding sophora alopecuroide into ethanol and soaking, performing reflux extraction, filtering remained filtrate and adding into the liquid A to obtain liquid which is recorded as liquid B; and (3) extracting volatile oil from angelica, pepper and cloves by using distilled water to obtain mixed volatile oil A and mixed extract, filtering and concentrating the mixed extract, performing alcohol precipitation, filtering, recovering alcohol to obtain filtrate, adding the volatile oil A and the filtrate into the liquid B, uniformly stirring and recording the liquid as liquid C, dissolving menthol and borneol by using ethanol and then adding into the liquid C, adjusting alcoholic strength, stirring, standing, filtering to obtain the filtrate, namely a finished product. The sophora alopecuroide skin bacteriostatic spray provided by the invention can be used for effectively treating common skin pruritus.
Description
Technical field
The present invention relates to a kind of Herba Sophorae alopecuroidis skin bacteriostasis spray and preparation method thereof.
Background technology
Skin pruritus is the common sympton in the daily life, and the cause of skin pruritus is very complicated, and common pruritus has pruritus due to wind pathogen, pruritus due to damp pathogen, insectspruritus, pruritus due to heat, pruritus due to dryness etc.Common clinically dermatitis, eczema, tinea etc. all have the pruritus symptom, and aspect treatment, the medicine of promoting blood circulation by removing wind commonly used, anti-inflammation and sterilization reaches antipruritic purpose.Treat clinically at present that the sick external used medicine of pruritus is antipruritic with calmness, skin care is a principle; Often select glucocorticoid example hydrochloric acid dexamethasone, halcinonidedcorten etc. for use, use for a long time and can make atrophoderma, telangiectasis; And the generation dependency, so require an amount of or discontinuity medication of medication.Select for use Chinese medicine can avoid the untoward reaction of above-mentioned Western medicine to skin pruritus, spray is the pharmacy type of using always.For example publication number is the production method that the one Chinese patent application of CN101444568 discloses a kind of skin bacteriostasis spray; Main processes is that Radix Angelicae Sinensis, Cortex Pseudolaricis, Rhizoma Chuanxiong, Cortex Phellodendri, Fructus Zanthoxyli, six kinds of medical material mixed powders of Herba Houttuyniae are broken into coarse powder; Use the alcohol heating reflux secondary, obtain herb liquid A; Herba Sophorae alopecuroidis total alkali liquid is condensed into extractum with heating in water bath, joins among the herb liquid A, stir, left standstill and filter medical liquid B; Flos Caryophylli powder is broken into coarse powder, adds the water reflux, extract volatile oil and comprise, get medicinal liquid C with Tween 80, glycerol; With dissolve with ethanol Herba Menthae, Borneolum Syntheticum, wait to dissolve back and medical liquid B and join among the medicinal liquid C, get product after stirring.The spray that this method makes has significant curative effect to the common skin pruritus.But responding time and healing time are still not ideal enough, remain further to be improved to improve therapeutic efficiency.
Summary of the invention
The purpose of this invention is to provide and a kind ofly can efficiently treat Herba Sophorae alopecuroidis skin bacteriostasis spray of common skin pruritus and preparation method thereof.
The method for preparing of Herba Sophorae alopecuroidis skin bacteriostasis spray provided by the invention may further comprise the steps:
(1) Herba Houttuyniae 50～70 weight portions, Rhizoma Chuanxiong 35～45 weight portions, Radix Et Rhizoma Rhei 35～45 weight portions, Herba Schizonepetae 50～70 weight portions are mixed in the adding extraction pot, use ethanol extraction, obtain total filtrating, be designated as liquid A;
(2) Herba Sophorae alopecuroidis 40～60 weight portions were added after 8～14 times the soak with ethanol that weight is Herba Sophorae alopecuroidis reflux, extract, 14～18 hours, filter the filtrating of leaving and taking and add in the liquid A, the liquid that obtains is designated as liquid B;
(3) Radix Angelicae Sinensis 25～35 weight portions, Pericarpium Zanthoxyli 30～40 weight portions, Flos Caryophylli 40～60 weight portions being used distilled water extraction volatile oil, get blended volatile oil A and blended extracting solution, is the liquid of 1.05～1.1g/ml with blended extracting liquid filtering, simmer down to density; Precipitate with ethanol filters recovered alcohol; Must filtrate, volatile oil A and filtrating joined in the liquid B again, the back liquid that stirs is designated as liquid C; Mentholum 3～10 weight portions, Borneolum Syntheticum 3～10 weight portions are added among the liquid C after with dissolve with ethanol; Regulate alcoholic strength, stir, leave standstill, filter, this filtrating is finished product.
Preferably, the consumption of each raw material components is following: Herba Houttuyniae 55～65 weight portions, Rhizoma Chuanxiong 35～45 weight portions, Radix Et Rhizoma Rhei 35～45 weight portions, Herba Schizonepetae 55～65 weight portions; Herba Sophorae alopecuroidis 45～55 weight portions; Radix Angelicae Sinensis 25～35 weight portions, Pericarpium Zanthoxyli 30～40 weight portions, Flos Caryophylli 45～55 weight portions, Mentholum 3～8 weight portions, Borneolum Syntheticum 3～8 weight portions.
More preferably, the consumption of each raw material components is following: Herba Houttuyniae 58～62 weight portions, Rhizoma Chuanxiong 33～42 weight portions, Radix Et Rhizoma Rhei 33～42 weight portions, Herba Schizonepetae 58～62 weight portions; Herba Sophorae alopecuroidis 48～52 weight portions; Radix Angelicae Sinensis 28～32 weight portions, Pericarpium Zanthoxyli 34～38 weight portions, Flos Caryophylli 46～50 weight portions, Mentholum 4～6 weight portions, Borneolum Syntheticum 4～6 weight portions.
Preferably, in the step (1), with ethanol extraction 1～3 time, each 1～2 hour, each ethanol that adds was said Herba Houttuyniae, Rhizoma Chuanxiong, Radix Et Rhizoma Rhei and Herba Schizonepetae gross weight 8～14 times.
Preferably, in the step (2), soak time is 1～3 hour.
Preferably, in the step (3), the method for preparing of said volatile oil A and filtrating is following: join Radix Angelicae Sinensis, Pericarpium Zanthoxyli, Flos Caryophylli in the volatile oil extractor respectively; Each adds 6～10 times of weight distilled water extraction; Extracted 6～10 hours, and collected and mix three kinds of volatile oil, blended volatile oil A; With extracting liq merging filtration, the simmer down to density of above-mentioned Radix Angelicae Sinensis, Flos Caryophylli and Pericarpium Zanthoxyli is liquid, the precipitate with ethanol of 1.05～1.1g/ml, filters, and recovered alcohol must be filtrated.
Preferably, the Herba Houttuyniae of using, Rhizoma Chuanxiong, Radix Et Rhizoma Rhei, Herba Schizonepetae, Herba Sophorae alopecuroidis, Radix Angelicae Sinensis, Pericarpium Zanthoxyli, Flos Caryophylli are 8～15 purpose coarse powder.
The spray that is got by above-mentioned any method preparation is Herba Sophorae alopecuroidis skin bacteriostasis spray provided by the invention.
Herba Sophorae alopecuroidis skin bacteriostasis spray provided by the invention can efficiently be treated common skin pruritus.This spray is with blood circulation promoting and blood stasis dispelling, and the detoxifcation of dispeling the wind is main, is aided with heat-clearing and toxic substances removing; The wind that looses is antipruritic, and antibacterial parasite killing reaches the purpose of alleviating the skin pruritus, because most of medicine is the article of cold ice; Be equipped with hot temperature medicine, alleviate cold and cool property; Make the medicine nonirritant, and use suffering loose the medicine of string, can reach priming return through purpose.
Description of drawings
Fig. 1 is the process chart of the method for preparing of Herba Sophorae alopecuroidis skin bacteriostasis spray of the present invention.
The specific embodiment
Skin pruritus or accumulate in skin because of wind heat wind and cold or damp and hot heresy must not catharsis, or is stayed for a long time in the body by ailment said due to cold or exposure; It is puckery that fire-transformation is given birth to thermic Tianjin blood depletion, and due to the failure of skin and muscle to be nourished, so the present invention is with Radix Angelicae Sinensis, Rhizoma Chuanxiong, Radix Et Rhizoma Rhei blood circulation promoting and blood stasis dispelling; The detoxifcation of dispeling the wind, antibacterial parasite killing, dispelling wind and heat pathogens, removing damp to relieve itching are ministerial drug, assistant is dispersed the cold and cool property of said medicine with Flos Caryophylli, Pericarpium Zanthoxyli; The nutrition skin is a main purpose, is equipped with Borneolum Syntheticum, Mentholum then with being intended to detoxify diffusing wind, anticorrosion tissue regeneration promoting, the messenger drug that means that row looses trouble.
Herba Sophorae alopecuroidis skin bacteriostasis spray provided by the invention is with blood circulation promoting and blood stasis dispelling, and the detoxifcation of dispeling the wind is main, is aided with heat-clearing and toxic substances removing; The wind that looses is antipruritic, and antibacterial parasite killing reaches the purpose of alleviating the skin pruritus, because most of medicine is the article of cold ice; Be equipped with hot temperature medicine, alleviate cold and cool property; Make the medicine nonirritant, and use suffering loose the medicine of string, mainly be for reach priming return through purpose.
As shown in Figure 1, the method for preparing of Herba Sophorae alopecuroidis skin bacteriostasis spray provided by the invention mainly may further comprise the steps:
(1) Herba Houttuyniae, Rhizoma Chuanxiong, Radix Et Rhizoma Rhei, Herba Schizonepetae are mixed in the adding extraction pot, use ethanol extraction, obtain total filtrating, be designated as liquid A;
(2) Herba Sophorae alopecuroidis is added reflux, extract, after the soak with ethanol, filter the filtrating of leaving and taking and add in the liquid A, the liquid that obtains is designated as liquid B;
(3) Radix Angelicae Sinensis, Pericarpium Zanthoxyli, Flos Caryophylli are used distilled water extraction volatile oil, get blended volatile oil A and blended extracting solution, blended extracting liquid filtering, concentrated, precipitate with ethanol; Filter, recovered alcohol must be filtrated; Volatile oil A and filtrating are joined in the liquid B, the back liquid that stirs is designated as liquid C again, and Mentholum, Borneolum Syntheticum are added among the liquid C after with dissolve with ethanol; Regulate alcoholic strength, stir, leave standstill, filter, this filtrating is finished product.
Through embodiment the method for preparing of Herba Sophorae alopecuroidis skin bacteriostasis spray of the present invention is further specified below, and combine Comparative Examples that the result of use of the Herba Sophorae alopecuroidis skin bacteriostasis spray that obtains is described.
The method for preparing of embodiment one Herba Sophorae alopecuroidis skin bacteriostasis spray (the consumption unit of each crude drug if no special instructions, all is a weight portion)
(1) 60 4 kinds of medical materials of Herba Houttuyniae 60, Rhizoma Chuanxiong 40, Radix Et Rhizoma Rhei 40, Herba Schizonepetae, the 10 purpose coarse powder after will cleaning mix and add in the extraction pot; The concentration that adds 12 times of weight is 80% ethanol, extracts twice, each 1.5 hours; Merge extracted twice liquid, filtration, filtrating are designated as liquid A.
(2) soak with ethanol of Herba Sophorae alopecuroidis 50 remove impurity, 12 times of weight of coarse powder (10 order) adding is put into the reflux, extract, device after 2 hours, reflux, extract, 16 hours is filtered the filtrating of leaving and taking and is added in the liquid A, and the liquid of this moment is designated as liquid B.
(3) 10 order coarse powder with Radix Angelicae Sinensis 30, Pericarpium Zanthoxyli 36, Flos Caryophylli 48 join respectively in the volatile oil extractor, and each adds 9 times of weight distilled water extraction, extract 8 hours; Collect and mix three kinds of volatile oil, be designated as volatile oil A, the extracting liq of above-mentioned Radix Angelicae Sinensis, Flos Caryophylli and Pericarpium Zanthoxyli is merged filter, simmer down to density is liquid, the precipitate with ethanol of 1.05g/ml and makes the liquid alcohol degree reach 60 °; Filter, recovered alcohol must be filtrated; Again volatile oil A and filtrating are joined in the liquid B; The back liquid that stirs is designated as liquid C, and the dissolving of the ethanol (95 weight %) of Mentholum 5, Borneolum Syntheticum 5 usefulness 200 weight portions is joined among the liquid C, and adding an amount of distilled water then, to regulate the alcoholic strength of liquid be 25 to spend; Stir, leave standstill 24 hours, filtration, this filtrating is finished product.
The method for preparing of embodiment two Herba Sophorae alopecuroidis skin bacteriostasis sprays (the consumption unit of each crude drug if no special instructions, all is a weight portion)
(1) 65 4 kinds of medical materials of Herba Houttuyniae 55, Rhizoma Chuanxiong 40, Radix Et Rhizoma Rhei 45, Herba Schizonepetae, the 10 purpose coarse powder after will cleaning mix and add in the extraction pot, add the ethanol of 80 weight % of 14 times of weight, extracted 2 hours, extracting solution, filter, filtrating is designated as liquid A.
(2) soak with ethanol of Herba Sophorae alopecuroidis 55 remove impurity, 10 times of weight of coarse powder (10 order) adding is put into the reflux, extract, device after 3 hours, reflux, extract, 14 hours is filtered the filtrating of leaving and taking and is added in the liquid A, and the liquid of this moment is designated as liquid B.
(3) 10 order coarse powder with Radix Angelicae Sinensis 35, Pericarpium Zanthoxyli 30, Flos Caryophylli 55 are mixed to join in the volatile oil extractor, add 8 times of weight distilled water extraction, extract 8 hours; Collect blended volatile oil, be designated as volatile oil A, with the extracting liq of above-mentioned Radix Angelicae Sinensis, Flos Caryophylli and Pericarpium Zanthoxyli filter, simmer down to density is liquid, the precipitate with ethanol of 1.1g/ml and makes the liquid alcohol degree reach 60 °; Filter, recovered alcohol must be filtrated; Again volatile oil A and filtrating are joined in the liquid B; The back liquid that stirs is designated as liquid C, and the dissolving of the ethanol (95 weight %) of Mentholum 3, Borneolum Syntheticum 8 usefulness 300 weight portions is joined among the liquid C, and adding an amount of distilled water then, to regulate the alcoholic strength of liquid be 25 to spend; Stir, leave standstill 24 hours, filtration, this filtrating is finished product.
Comparative Examples (according to publication number is the disclosed content setting of one Chinese patent application of CN101444568, and if no special instructions, unit is a weight portion)
(1) the medical material Radix Angelicae Sinensis after will cleaning, Cortex Pseudolaricis, Rhizoma Chuanxiong, Cortex Phellodendri, Fructus Zanthoxyli, Herba Houttuyniae were by 1: 1: 1.5: 1.8: 1.8: 3 weight ratio mixed powder is broken into coarse powder; Alcohol heating reflux secondary with concentration 80%; Add for the first time the ethanol of 6 times of weight, extracted 1.5 hours, add the ethanol extraction 1 hour of 4 times of gross weights the second time; Merge extracted twice liquid, obtain herb liquid A;
(2) Herba Sophorae alopecuroidis total alkali liquid is condensed into extractum with heating in water bath, joins among the herb liquid A, stir, leave standstill 48 hours after-filtration and get liquor B;
(3) Flos Caryophylli powder is broken into coarse powder, adds the water reflux of 10 times of weights, extract volatile oil, all volatile oil are comprised with Tween 80, glycerol, get medicinal liquid C with volatile oil extractor; The dissolve with ethanol Herba Menthae of the concentration that takes a morsel 95%, Borneolum Syntheticum; Treating to dissolve fully back and medicinal liquid slowly joins among the medicinal liquid C; The limit edged stirs; The bacteriostasis spray finished product that after stirring is wherein contains medicinal liquid A90 (weight portion, down together), Herba Sophorae alopecuroidis total alkali extractum 4, Tween 80 3, glycerol 2, Mentholum 0.6, Borneolum Syntheticum 0.6, Flos Caryophylli volatile oil 0.35.
The bacteriostatic experiment effect of three kinds of products that the foregoing description one, embodiment two and Comparative Examples obtain is described below.
One, escherichia coli bacteriostatic experiment
(1) material:
1, test strain: in the 6th generation of escherichia coli (8099), grinding institute by military medical courses in general institute stream provides.
2. disinfectant: be respectively the skin bacteriostasis spray that embodiment one, two and Comparative Examples make
3. culture medium: nutrient broth, Nutrient agar is by the conventional method preparation
4. diluent (PBS)
(2) method:
1, test basis: " disinfection technology standard " 2002 editions 2.1.11.3.2 items
2, test uses disinfectant to be stock solution
3, experimental temperature is 19～22 ℃, experiment repetition 3 times
(3) result:
The escherichia coli fungistatic effect of the product that Comparative Examples makes
The escherichia coli fungistatic effect of the product that embodiment one makes
The escherichia coli fungistatic effect of the product that embodiment two makes
(4), conclusion: according to " disposable use hygienic article sanitary standard " (GB15979-2002), the Herba Sophorae alopecuroidis skin bacteriostasis spray is under experimental condition, and stock solution effect 2min to colibacillary bacteriostasis rate＞50%, has bacteriostasis.
Two, staphylococcus aureus bacteriostatic experiment
(1) material:
1, test strain: in the 6th generation of staphylococcus aureus (ATCC6538), grinding institute by military medical courses in general institute stream provides.
2. disinfectant: be respectively the antibacterial liquid that embodiment one, two and Comparative Examples make
3. culture medium: nutrient broth, Nutrient agar
4. diluent (PBS)
(2) method:
1, test basis: " disinfection technology standard " 2002 editions 2.1.11.3.2 items
2, test uses disinfectant to be stock solution
3, experimental temperature is 19～22 ℃, experiment repetition 3 times
(3) result:
The staphylococcus aureus fungistatic effect of the product that Comparative Examples makes
The staphylococcus aureus fungistatic effect of the product that embodiment one makes
The staphylococcus aureus fungistatic effect of the product that embodiment two makes
(4), conclusion: according to " disposable use hygienic article sanitary standard " (GB15979-2002), the Herba Sophorae alopecuroidis skin bacteriostasis spray under experimental condition, stock solution effect 2min, bacteriostasis rate＞50% to staphylococcus aureus has bacteriostasis.
Three, Candida albicans bacteriostatic experiment
(1) material:
1, experimental strain: in the 5th generation of Candida albicans (ATCC10231), provide by microorganism fungus kind preservation center, Guangdong Microbes Inst
2. disinfectant: be respectively the antibacterial liquid that embodiment one, two and Comparative Examples make
3. culture medium: husky fort fluid medium, husky fort agar culture medium
4, diluent (PBS)
(2) method:
1, test basis: " disinfection technology standard " 2002 editions 2.1.11.3.2 items
2, test uses disinfectant to be stock solution
3, experimental temperature is 20～22 ℃, experiment repetition 3 times
(3) result:
The Candida albicans fungistatic effect of the product that Comparative Examples makes
The Candida albicans fungistatic effect of the product that embodiment one makes
The Candida albicans fungistatic effect of the product that embodiment two makes
(4), conclusion: according to " disposable use hygienic article sanitary standard " (GB15979-2002), the Herba Sophorae alopecuroidis skin bacteriostasis spray is under experimental condition, and stock solution effect 2min to the oidiomycetic bacteriostasis rate of white＞50%, has bacteriostasis.
In addition, through clinical showing, the Herba Sophorae alopecuroidis skin bacteriostasis spray of method preparation of the present invention has significant curative effect to common skin pruritus.Be illustrated below.
1. patient: Lee; Sex: woman; Age: 34 years old;
Disease is described: the red pimple of grain of rice size appears in the arm inboard, and scratchy, the scratching that does not stop causes skin peeling, redness, incrustation
Therapeutic Method: clean the affected part, get the product that embodiment one makes and be sprayed on the affected part, every day 3-5 time.Three minutes take effect.Three days is a course of treatment
Therapeutic effect: use pruritus transference cure after a day, all symptoms all disappear after two courses of treatment, thoroughly cure, not recurrence again
2. patient: Zheng; Sex: man; Age: 18 years old;
Disease is described: the family humid environment, and cause shank goose pimples to occur, red, very itch, grab and more itch.
Therapeutic Method: Therapeutic Method: clean the affected part, get the product that embodiment one makes and be sprayed on the affected part, every day 3-5 time.Three minutes take effect.Three days is a course of treatment
Therapeutic effect: use one day pruritus transference cure, all symptoms all disappear after two courses of treatment, and thoroughly cure three courses of treatment, not recurrence again,
3. patient: Zhang; Sex: man; Age: 50 years old;
Disease is described: summer mosquito bite
Therapeutic Method: Therapeutic Method: clean the affected part, get the product that embodiment two makes and be sprayed on the affected part, every day 3-5 time.Three minutes take effect.Three days is a course of treatment
Therapeutic effect: use one day pruritus transference cure
4, patient: Chen; Sex: man; 27 years old age;
Symptom is described: some point-like macules appear in arm, ancon
Therapeutic Method: clean the affected part, get the product that embodiment two makes and be sprayed on the affected part, every day 3-5 time.Three minutes take effect.Three days is a course of treatment.
Therapeutic effect: using two days later, the point-like macule disappears.
Claims (8)
1. the method for preparing of Herba Sophorae alopecuroidis skin bacteriostasis spray is characterized in that, may further comprise the steps:
(1) Herba Houttuyniae 50～70 weight portions, Rhizoma Chuanxiong 35～45 weight portions, Radix Et Rhizoma Rhei 35～45 weight portions, Herba Schizonepetae 50～70 weight portions are mixed in the adding extraction pot, use ethanol extraction, obtain total filtrating, be designated as liquid A;
(2) Herba Sophorae alopecuroidis 40～60 weight portions were added after 8～14 times the soak with ethanol that weight is Herba Sophorae alopecuroidis reflux, extract, 14～18 hours, filter the filtrating of leaving and taking and add in the liquid A, the liquid that obtains is designated as liquid B;
(3) Radix Angelicae Sinensis 25～35 weight portions, Pericarpium Zanthoxyli 30～40 weight portions, Flos Caryophylli 40～60 weight portions being used distilled water extraction volatile oil, get blended volatile oil A and blended extracting solution, is the liquid of 1.05～1.1g/ml with blended extracting liquid filtering, simmer down to density; Precipitate with ethanol filters recovered alcohol; Must filtrate, volatile oil A and filtrating joined in the liquid B again, the back liquid that stirs is designated as liquid C; Mentholum 3～10 weight portions, Borneolum Syntheticum 3～10 weight portions are added among the liquid C after with dissolve with ethanol; Regulate alcoholic strength, stir, leave standstill, filter, this filtrating is finished product.
2. the method for preparing of Herba Sophorae alopecuroidis skin bacteriostasis spray according to claim 1 is characterized in that, the consumption of each raw material components is following: Herba Houttuyniae 55～65 weight portions, Rhizoma Chuanxiong 35～45 weight portions, Radix Et Rhizoma Rhei 35～45 weight portions, Herba Schizonepetae 55～65 weight portions; Herba Sophorae alopecuroidis 45～55 weight portions; Radix Angelicae Sinensis 25～35 weight portions, Pericarpium Zanthoxyli 30～40 weight portions, Flos Caryophylli 45～55 weight portions, Mentholum 3～8 weight portions, Borneolum Syntheticum 3～8 weight portions.
3. the method for preparing of Herba Sophorae alopecuroidis skin bacteriostasis spray according to claim 1 is characterized in that, the consumption of each raw material components is following: Herba Houttuyniae 58～62 weight portions, Rhizoma Chuanxiong 33～42 weight portions, Radix Et Rhizoma Rhei 33～42 weight portions, Herba Schizonepetae 58～62 weight portions; Herba Sophorae alopecuroidis 48～52 weight portions; Radix Angelicae Sinensis 28～32 weight portions, Pericarpium Zanthoxyli 34～38 weight portions, Flos Caryophylli 46～50 weight portions, Mentholum 4～6 weight portions, Borneolum Syntheticum 4～6 weight portions.
4. the method for preparing of Herba Sophorae alopecuroidis skin bacteriostasis spray according to claim 1; It is characterized in that, in the step (1), with ethanol extraction 1～3 time; Each 1～2 hour, each ethanol that adds was said Herba Houttuyniae, Rhizoma Chuanxiong, Radix Et Rhizoma Rhei and Herba Schizonepetae gross weight 8～14 times.
5. the method for preparing of Herba Sophorae alopecuroidis skin bacteriostasis spray according to claim 1 is characterized in that, in the step (2), soak time is 1～3 hour.
6. the method for preparing of Herba Sophorae alopecuroidis skin bacteriostasis spray according to claim 1; It is characterized in that in the step (3), the method for preparing of said volatile oil A and filtrating is following: join Radix Angelicae Sinensis, Pericarpium Zanthoxyli, Flos Caryophylli in the volatile oil extractor respectively; Each adds 6～10 times of weight distilled water extraction; Extracted 6～10 hours, and collected and mix three kinds of volatile oil, blended volatile oil A; With extracting liq merging filtration, the simmer down to density of above-mentioned Radix Angelicae Sinensis, Flos Caryophylli and Pericarpium Zanthoxyli is liquid, the precipitate with ethanol of 1.05～1.1g/ml, filters, and recovered alcohol must be filtrated.
7. the method for preparing of Herba Sophorae alopecuroidis skin bacteriostasis spray according to claim 1 is characterized in that, the Herba Houttuyniae of using, Rhizoma Chuanxiong, Radix Et Rhizoma Rhei, Herba Schizonepetae, Herba Sophorae alopecuroidis, Radix Angelicae Sinensis, Pericarpium Zanthoxyli, Flos Caryophylli are 8～15 purpose coarse powder.
8. the Herba Sophorae alopecuroidis skin bacteriostasis spray is characterized in that, this Herba Sophorae alopecuroidis skin bacteriostasis spray is got by each described method preparation of claim 1～7.
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