CN102258544B - Use of pine needle extract in preparation of medicines or health-care products for promoting mercury discharge - Google Patents
Use of pine needle extract in preparation of medicines or health-care products for promoting mercury discharge Download PDFInfo
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- CN102258544B CN102258544B CN2011102460947A CN201110246094A CN102258544B CN 102258544 B CN102258544 B CN 102258544B CN 2011102460947 A CN2011102460947 A CN 2011102460947A CN 201110246094 A CN201110246094 A CN 201110246094A CN 102258544 B CN102258544 B CN 102258544B
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- mercury
- hydrargyrum
- pine needle
- folium pini
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Abstract
The invention discloses the use of pine needle extract in the preparation of medicines or health-care products for promoting mercury discharge. The pine needle extract is prepared by using pine needles as a raw material and by cutting, drying, grinding, extracting in nonpolar solvent, concentrating, saponifying and the like. Animal experiments indicate that the pine needle extract can obviously lower the content of mercury in renal cortex, liver and urine in rats contaminated by mercury and urea nitrogen content in the serum of the rats contaminated by mercury and has the functions of preventing mercury accumulation in body and improving functions of kidney. Therefore, the pine needle extract can be used for preparing the medicines or health-care products for promoting mercury discharge from bodies.
Description
Technical field
The present invention relates to a kind of medicine or health product that promote the effect of row's hydrargyrum that have, relate to the medicine of extract from pine needles row's hydrargyrum effect in preparation promotes body or the application in the health product specifically, belong to the Medicines and Health Product field.
Background technology
Hydrargyrum is commonly called as hydrargyrum, is unique metallic element that is in a liquid state at normal temperatures, and is very serious to the harm of human body, become a global pollution problem.About 10,000 tons of the hydrargyrum amount of the annual product in the whole world, and every year is with 2% speed increase.Industrial, hydrargyrum is mainly used in aspects such as chlor-alkali, electric meter, paint, catalyst, reactor cooling, precious metal smelting, aneroid battery, coating, paper pulp.On agricultural, hydrargyrum is mainly as vegetable insecticide, soil insecticide and seed disinfectant etc.Militarily, as mercury fulminate, can be used as detonating agent.Pharmaceutically, hydrargyrum can be used as antibacterial, diuretic, Chinese medicine, contraceptive, dental material etc.In daily life, hydrargyrum can be used for thermometer, piezometer, finsen lamp, fluorescent lamp, sphygomanometer, cosmetics etc.Therefore, the chemical compound of hydrargyrum and hydrargyrum is used very extensive in industry, agricultural, medicine, daily life.The hydrargyrum of the overwhelming majority finally enters environment with " three wastes " form after use.Along with the fast development of modern industry, the purposes of hydrargyrum more and more widely, the pollution problem of hydrargyrum is serious day by day, has become public's medical problem of having to pay attention in various countries, the world today.
The main path that inorganic mercury enters in the body is breathing, oral cavity picked-up and skin absorbs.Breathing be mercury vapour enter human body most important approach, suck about 80% of mercury vapour and can see through alveolar and enter blood.Inorganic mercury in the food nearly 7% is absorbed by oral cavity picked-up, the mercury vapour by skin absorbs only be by breathe absorb about 1%, but be to use the Skin whitening care cosmetics of some high inorganic mercury content also can cause hydrargyrum to absorb and accumulation.The toxicity of inorganic mercury mainly shows as neurotoxicity and Toxicity of Kidney.The central nervous system may be the target organ the most responsive to mercury vapour, its take place classical symptom after mercurialism comprise tremble, emotional instability, absent minded, insomnia, amnesia, tremble in a minute, blurred vision, the variation of muscle function of nervous system, headache and comprehensive dysautonomia etc.Kidney is the same with the central nervous system, also is the target organ to the mercury vapour sensitivity.The adverse consequences of human body mercurialism also comprises carcinogenecity, respiratory system toxicity, Cardiovascular Toxicity, digestive system toxicity, immune system toxicity, dermal toxicity and reproductive system toxicity etc.The main path that organic mercuries such as methyl mercury enter human body is edible fishes and other aquatic products.Methyl mercury very easily is absorbed and is distributed to whole body in human body intestinal canal, major part is accumulated in liver and the kidney, and the methyl mercury that is distributed in the cerebral tissue accounts for 15%, but cerebral tissue is undermined then prior to other each tissue.Main damaging part is cerebral cortex, cerebellum and peripheral nerve.One of serious nuisance disease " Mad Hatter's disease " is exactly caused by the water body environment mercury pollution in the world.
On the clinical treatment of mercurialism, mainly be to utilize the metal-chelator dimercapto third sulphur sodium (DMPS) at present, the mercury ion in the substituted radical forms the hydrargyrum complex and discharges from urine.But this medicine is intravenous injection, makes troubles to the patient, and certain toxic and side effects is arranged, and raises on the contrary as microdose urine protein after using DMPS and driving hydrargyrum, and the trend that increases the weight of injury of kidney is arranged, and as prophylactic certain shortcoming is arranged.Therefore, development safety, mercurialism control medicine convenient, efficient, low toxicity have important practical significance.
Folium Pini is the needle of pinaceae plant, has another name called needle, Folium Pini, Folium Pini etc., at the existing long edible and medicinal history of China.Famous physician's Li Shizhen (1518-1593 A.D.) is put down in writing in Compendium of Material Medica: " nontoxic, for a long time clothes make us not old for Folium Pini, abnormal smells from the patient hardship, temperature, and the QI invigorating of making light of one's life by commiting suicide cures mainly the rheumatism skin ulcer, piliation, and settling five organs, in keeping, hungry prolonging life not "." the national Chinese herbal medicine compilation " edited and publish modern age summed up experience among the people: " Folium Pini has expelling wind and activating blood circulation, makes eye bright and calm the nerves, functions such as detoxifying and relieving itching " can be used to treat influenza, rheumatic arthritis, nyctalopia, hypertension, the neurasthenia, external curing chilblain etc.Modern pharmacological research shows, Folium Pini and extract thereof have calmness, analgesia, analgesic, antiinflammatory, antitussive, eliminate the phlegm, slow down aging, mutation, blood fat reducing, blood sugar lowering, cholesterol reducing, function of gallbladder promoting blood pressure lowering, effect such as antibacterial.Do not see bibliographical information but whether Folium Pini or its extract have the effect of the interior row of the body of promotion hydrargyrum.
Summary of the invention
Purpose of the present invention is exactly in order to overcome at present in prevention and treatment mercurialism, except injecting chelating agent as the shortcoming of still not having the specific treatment medicine the detoxifcation, particularly prevent chronic mercury poisoning, provide a kind of extract from pine needles to have the medicine of row's hydrargyrum effect or the application in the health product in preparation.
Technical scheme of the present invention is: extract from pine needles promotes the medicine of row's hydrargyrum effect or the application in the health product in preparation.
Zoopery demonstration of the present invention, extract from pine needles can significantly reduce dyes hydrargyrum kidney of rats cortex hydrargyrum, liver hydrargyrum, urine hydrargyrum and serum urea nitrogen content, has the effect of removing the interior mercury accumulation of body and improving renal function.Therefore, extract from pine needles can be used for preparing medicine or the health product that promote row's hydrargyrum effect in the body.
The present invention is effective ingredient with the extract from pine needles, be equipped with pharmaceutically acceptable carrier or excipient, technology is made pharmaceutical dosage forms such as tablet, capsule, electuary, granule, oral liquid, chewable tablet routinely, also can with the composite compound preparation of making of other medicines composition.
Beneficial effect:
1, the present invention has opened up a new medical application fields for the comprehensive utilization of Folium Pini opens up a new way.Adopt conventional method of analysis, the key technical indexes of extract from pine needles is: 1. dry matter content 〉=55%, 2. pH value (1% aqueous solution): 8.0～10.0,3. content of ashes≤4%, 4. content beta-carotene 〉=500mg/kg, 5. chlorophyll content 〉=3500mg/kg, 6. content of vitamin E 〉=5000mg/kg, 7. water insoluble volatile content≤4%.
2, the present invention selects Folium Pini as raw material, and the medicine source is abundant, and it is wide to originate, develop with low cost, contaminated few and from forest, have no side effect, belong to natural product, have good especially physiological adaption and safety.
3, extract from pine needles of the present invention is promoting to have obvious effect aspect row's hydrargyrum, alleviation or the treatment mercurialism, can be made into tablet, capsule, electuary, granule, oral liquid, chewable tablet etc., taking convenience, can be used for the hydrargyrum operating worker daily drive the hydrargyrum medicine, also be fit to row's hydrargyrum health product that the serious regional personnel of mercury pollution take for a long time.
The specific embodiment
The present invention is described in further detail below in conjunction with embodiment, but they are not limitation of the present invention.
Extract from pine needles of the present invention contains abundant chlorophyll and bioactive substances such as derivant, carotenoid, multivitamin (E, K, D), polyprenol, terpenoid and plant sterol thereof, be that browse with pinaceae plants such as Pinus, Picea, Abies or Cedrus is raw material, through chopping, dry, pulverize after, with non-polar solven by the extract from pine needles that extracts, concentrates, step such as saponification is made.Described non-polar solven is any one in petroleum ether, n-hexane, No. 6 solvent naphthas.Specifically can reference name be called preparation method and the application in preparation treatment gastric ulcer medicine thereof of Folium Pini liposoluble extract, application number is the method preparation of putting down in writing in 201010298504.8 the application.
Embodiment 1: extract from pine needles extracts
Extract from pine needles extracts, and is the method for record in 201010298504.8 with application number, is raw material with the Folium Pini, through chopping, dry, pulverize after, with non-polar solven by the extract from pine needles that extracts, concentrates, step such as saponification is made.
Embodiment 2: the extract from pine needles tablet
Extract from pine needles adds adjuvants such as maltodextrin, microcrystalline Cellulose, polydextrose, xylitol, citric acid and fully mixes, and the back of granulating adds magnesium stearate and the spice tabletting is made tablet.
Embodiment 3: the extract from pine needles capsule
Extract from pine needles adds medical starch, oligomeric xylose, sodium carboxymethyl cellulose, edible calcium carbonate, and adjuvants such as magnesium stearate fully mix, and makes capsule with the capsule loading machine Capsules of packing into.
Embodiment 4: extract from pine needles is removed the interior mercury accumulation of body and is improved the effect of renal function
Get 100 of healthy SPF level rats, after 1 week of observation is no abnormal, be divided into the blank group at random, dye the hydrargyrum model group, extract from pine needles 200mg/kg, 400mg/kg, 3 dosage groups of 800mg/kg, 20 every group, male and female half and half.Each dosage group of extract from pine needles is gastric infusion in advance, once a day, continues 28d.Except blank group subcutaneous injection deionized water, other the 4 groups of equal subcutaneous injection 2.5mg/kg of rat HgCl
2Solution causes and dyes mercurialism.Behind the contamination 12h, rat is moved in the metabolic cage, only supply with the drink deionized water during this period, stop provand, collect the 12h urine sample, be used for measuring the content of urine hydrargyrum.Behind contamination 48h, the etherization rat, dorsal position is fixed on the operating board.Cut off the abdominal part fur, from ventral aorta blood sampling 5ml, leave standstill 1h after, 3000 rev/mins of centrifugalize serum are used for measuring serum urea nitrogen (BUN) content.After rat blood-letting behind the collection blood is deadly, cut liver and kidney, insert in the normal saline and wash.Accurately take by weighing liver, each 0.5 g of renal cortex, add 4ml KCl solution, with 10000 rev/mins of preparations of electronic refiner homogenate, 10 seconds/time, intermittently 20 seconds, continuous 5 times, in frozen water, carry out, grind to form the homogenate of 10 %.The homogenate tissue is pulverized with ultrasonic cell disruptor, be used for measuring renal cortex and liver mercury content.Serum BUN Determination on content adopts the blood urea nitrogen test kit, and the mensuration of urine, renal cortex and liver mercury content adopts cold-vapour atomic absorption method, the results are shown in Table 1.
Table 1 respectively organize serum BUN, urine, renal cortex regulating liver-QI mercury content relatively (
-x±
s, n=10)
Annotate:
*For with blank group ratio
P＜0.01;
#For with the model control group ratio
P＜0.05;
##For with the model control group ratio
P＜0.01.
As seen from Table 1, dye hydrargyrum model group kidney of rats cortex hydrargyrum and be the liver mercury content 10 times, visible renal cortex be hydrargyrum mainly accumulate the position; Dye hydrargyrum model group rat blood serum BUN content be significantly higher than the blank group (
P＜0.01), HgCl is described
2Caused the rat acute injury of kidney.With dye the hydrargyrum model group relatively, extract from pine needles 400mg/kg and 800 mg/kg dosage groups can make rat blood serum BUN content significantly reduce (
P＜0.05,
P＜0.01), the prompting extract from pine needles has certain preventive and therapeutic effect to injury of kidney.
As seen from Table 1, with the blank group relatively, dye hydrargyrum model group rat urine, renal cortex regulating liver-QI mercury content all significantly increase (
P＜0.01), illustrates that the mercurialism model prepares successfully.With dye the hydrargyrum model group relatively, extract from pine needles 400mg/kg and 800 mg/kg dosage groups can make rat urine, renal cortex regulating liver-QI mercury content significantly reduce (
P＜0.05,
P＜0.01), the prompting extract from pine needles has the effect of removing mercury accumulation in the body.
Therefore, extract from pine needles has remarkable effect aspect prevention and the treatment mercurialism, can be used for preparing the medicine or the health product that promote row's hydrargyrum effect in the body.
Claims (1)
1. extract from pine needles promotes the medicine of row's hydrargyrum effect or the application in the health product in preparation, it is characterized in that, described extract from pine needles preparation method is: be raw material with the Folium Pini, through chopping, dry, pulverize, extract, concentrate, saponification and Jia Shui be prepared from, concrete operations condition and step are as follows:
Step 1: gather fresh Folium Pini, be cut into 1～3cm after, when being dried to moisture content≤10%wt, pulverizing is 10～60 purpose pine needle powder again;
Step 2: be that 1:4～10 adds non-polar solvens in pine needle powder weight and solvent volume ratio, reflux, extract, is 1～2 time under 50～70 ℃ of conditions, and each 2～6h filters, merge extractive liquid,, and concentrating under reduced pressure obtains the Folium Pini liposoluble substance after reclaiming solvent;
Step 3: with the sodium hydroxide solution saponification Folium Pini liposoluble substance of 10～40%wt, pH value is controlled between 8～9, under 50～70 ℃ of conditions, behind saponification 1～2h, adds water and stirs, and makes the Folium Pini liposoluble extract of dry matter content 〉=55%wt.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101972284A (en) * | 2010-09-27 | 2011-02-16 | 中国林业科学研究院林产化学工业研究所 | Preparation method of pine needle fat soluble extract and application thereof in preparing drug for treating gastric ulcer |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101972284A (en) * | 2010-09-27 | 2011-02-16 | 中国林业科学研究院林产化学工业研究所 | Preparation method of pine needle fat soluble extract and application thereof in preparing drug for treating gastric ulcer |
Non-Patent Citations (1)
| Title |
|---|
| 李亚贤等.杜松针叶营养成份分析.《牡丹江师范学院学报(自然科学版)》.2001,(第04期),21-22. * |
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