CN102253104A - Automatic cleaning and glue pouring platform for capillary array - Google Patents
Automatic cleaning and glue pouring platform for capillary array Download PDFInfo
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- CN102253104A CN102253104A CN2011100732542A CN201110073254A CN102253104A CN 102253104 A CN102253104 A CN 102253104A CN 2011100732542 A CN2011100732542 A CN 2011100732542A CN 201110073254 A CN201110073254 A CN 201110073254A CN 102253104 A CN102253104 A CN 102253104A
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- capillary
- microsyringe
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- capillary array
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Abstract
The invention discloses an automatic cleaning and glue pouring platform for a capillary array. A push rod is arranged on an electric translation stage; a pressure sensor is arranged on an end surface of the push rod; a microscale sample injector support and a microscale sample injector are arranged on a direction on which the push rod travels; a tail end of the microscale sample injector is clinging to the pressure sensor, and a head of the microscale sample injector is connected with a conduit. A capillary support comprises a capillary head pedestal and a capillary central support, which are separated, wherein a height of the central support can be adjusted manually. A first path of a transparent tee joint is connected with the head of the microscale sample injector through a conduit; a second path is connected with a head of a capillary; and the third path is connected with a liquid bath liquid bath through a conduit. A pressure sensor display meter is disposed on a platform. The translation stage is equipped with a meter controller with a faceplate, a stepping motor and a direct-current power supply thereon. The platform of the invention can carry out repetitive automatic cleaning and glue pouring on the capillary array, and has advantages of high degree of automation, high cleaning and glue pouring efficiency, compact structure, simple operation and reliable performance, etc.
Description
Technical field
The present invention relates to bioassay technique, particularly relate to a kind of capillary array and clean and the encapsulating platform automatically.
Background technology
Capillary Electrophoresis is that sample moves in kapillary under effect of electric field, and different compositions has different migration velocities in the sample, realizes separating, and surveys in kapillary, by the signal of surveying sample is analyzed.This technology is widely used in biochemical analysis; as DNA, Separation of Proteins analysis; its separation efficiency height, sample consumption less, easily realize robotization, and it has a wide range of applications in dna sequencing, biology and fields such as clinical research, legal medical expert's evaluation, environmental protection, analytical chemistry and food inspection.
At present to the cleaning and the encapsulating of capillary array, employing be directly injection or semi-automatic work mode, have that automated procedures are not high, efficient is low, distinct disadvantage such as waste sample cause course of work length consuming time, cleaning performance instability, and need more manpower.
In order to realize high throughput analysis, many capillaries can be lined up array electrophoresis simultaneously, carry out parallel detection.In order to improve the automaticity of detection, be convenient to manually-operated, accelerate the speed of detection, effectively improve the resolution that detects, need before to capillary array electrophoresis, capillary array be cleaned and encapsulating fast and effectively.
Summary of the invention
The object of the present invention is to provide a kind of capillary array automatically to clean and the encapsulating platform, it has automated procedures height, cleaning and cleans automatically and the encapsulating platform with the capillary array that encapsulating speed is fast, controllability is strong, operating process is reliable and stable.
The technical solution used in the present invention is:
The present invention includes at the bottom of instrument controller, stepper motor driver, direct supply, pressure transducer Displaying Meter and the platform of motorized precision translation stage, push rod, pressure transducer, microsyringe support, microsyringe, capillary tube holder, threeway, capillary array, waste liquid tank, sample introduction conduit, waste discharge conduit, zone face plate; In platform base one side motorized precision translation stage, stepper motor and direct supply are installed, the instrument controller of zone face plate is connected with direct supply with stepper motor driver, one end and the stepper motor of motorized precision translation stage, the other end of motorized precision translation stage is connected with an end of push rod, on the other end of push rod pressure transducer is installed; At the platform base opposite side microsyringe support, capillary tube holder and pressure transducer Displaying Meter are installed; Be placed with microsyringe on the microsyringe support, the tail end of microsyringe can be close to pressure transducer, and the head of microsyringe links to each other with an end of sample introduction conduit, and the other end of sample introduction conduit links to each other with first end of threeway; Be placed with capillary array on the capillary tube holder, an end of capillary array links to each other with second end of threeway, and the other end of capillary array is water receiving tank behind the capillary head base; The 3rd end of threeway links to each other with an end of waste discharge conduit, and the other end of waste discharge conduit links to each other with waste liquid tank; The pressure transducer Displaying Meter links to each other with pressure transducer by circuit.
Described microsyringe support comprises end support and holder,head; End support and holder,head are used for fixing microsyringe.
Described capillary tube holder comprises that capillary head base and capillary midsection support are used for fixing capillary array, and the capillary midsection support is installed on the platform base, and adjustable height.
The beneficial effect that the present invention has is:
Capillary array of the present invention automatically cleans and the encapsulating platform, and the automatic cleaning and the encapsulating that can carry out repetition to capillary array have advantages such as automaticity height, cleaning and encapsulating efficient height, compact conformation, simple to operate, dependable performance.
Description of drawings
Fig. 1 is a structural principle synoptic diagram of the present invention.
Fig. 2 is a perspective view of the present invention.
Among the figure: 1, motorized precision translation stage, 2, push rod, 3, pressure transducer, 4a, end support, 4b, holder,head, 5, microsyringe, 6, capillary tube holder, 6a, capillary head base, 6b, capillary midsection support, 7, threeway, 8, capillary array, 9, waste liquid tank, 10a, sample introduction conduit, 10b, waste discharge conduit, 11, the instrument controller of zone face plate, 12, stepper motor driver, 13, direct supply, 14, the pressure transducer Displaying Meter, 15, platform base.
Embodiment
The invention will be further described below in conjunction with drawings and Examples.
As shown in Figure 1 and Figure 2, the present invention includes instrument controller 11, stepper motor driver 12, direct supply 13, pressure transducer Displaying Meter 14 and the platform base 15 of motorized precision translation stage 1, push rod 2, pressure transducer 3, microsyringe support, end support 4a, holder,head 4b, microsyringe 5, capillary tube holder 6, capillary head base 6a, capillary midsection support 6b, threeway 7, capillary array 8, waste liquid tank 9, sample introduction conduit 10a, waste discharge conduit 10b, zone face plate; In platform base 15 1 sides motorized precision translation stage 1, stepper motor and direct supply 13 are installed, the instrument controller 11 of zone face plate is connected with direct supply 13 with stepper motor driver 12, one end and the stepper motor of motorized precision translation stage 1, the other end of motorized precision translation stage 1 is connected with an end of push rod 2, and pressure transducer 3 is installed on the other end of push rod; At platform base 15 opposite sides microsyringe support, capillary tube holder 6 and pressure transducer Displaying Meter 14 are installed; Be placed with microsyringe 5 on the microsyringe support, the tail end of microsyringe 5 can be close to pressure transducer 3, and the head of microsyringe 5 links to each other with the end of sample introduction conduit 10a, and the other end of sample introduction conduit 10a links to each other with first end of threeway 7; Be placed with capillary array 8 on the capillary tube holder 6, an end of capillary array 8 links to each other with second end of threeway 7, and the other end of capillary array 8 water receiving tank behind capillary head base 6a is used to outpour liquid; The 3rd end of threeway 7 links to each other with the end of waste discharge conduit 10b, and the other end of waste discharge conduit 10b links to each other with waste liquid tank 9; Pressure transducer Displaying Meter 14 links to each other with pressure transducer 3 by circuit.
Described microsyringe support 4 comprises end support 4a and holder,head 4b; End support 4a and holder,head 4b are used for fixing microsyringe 5.Adopt the mode of the microsyringe embedded arrangement in 5 two ends, and tail end has detachable briquetting.
Described capillary tube holder 6 comprises that capillary head base 6a and capillary midsection support 6b are used for fixing capillary array 8, and capillary midsection support 6b is installed on the platform base 15, and adjustable height.
Wherein, the scope that moves forward and backward of push rod 2 is limited by limit switch, to guarantee that push rod 2 promotes microsyringe 5 and outpours liquid in controlled range.The middle part of capillary array 8 rides on the adjustable for height capillary tube holder 6, to guarantee capillary array 8 position suitables and to be difficult for being rolled over.
Workflow of the present invention is as follows:
(1) connects direct supply 13.On the panel of the instrument controller 11 of zone face plate, running parameter is set, by institute's setting parameter work, drives motorized precision translation stage 1, and then drive push rod 2 and move forward and backward by stepper motor driver 12 drive stepping motor.When push rod 2 moves forward, can contact the tail end of microsyringe 5, promote microsyringe 5 and outpour liquid, enter capillary array 8 after liquid process sample introduction conduit 10a, the threeway 7.Setting pressure sensor 3 between push rod 2 and microsyringe 5 tail ends, when push rod 6 promotes microsyringe 5, force value shows by pressure transducer Displaying Meter 14, and feed back to instrument controller, can leakage, obstruction have been judged whether according to force value, or normal, clean or the encapsulating process thereby be convenient to control.
(2) take out microsyringes 5 from microsyringe support 4, lay back (1) operation set by step again after drawing a certain amount of deionized water.
(3) repeating step (2) several times can be finished automatic cleaning process.
(4) take out microsyringes 5 from microsyringe support 4, lay back after drawing a certain amount of reagent again, (1) operation set by step is to drive the water in the kapillary out of.And then (1) operation set by step, can finish automatic encapsulating.
Above-mentioned just to the explaining of the embodiment of the invention, and be not limitation of the invention, every in essential scope of the present invention, all will fall within protection scope of the present invention variation, the modification of above embodiment.
Claims (3)
1. a capillary array cleans and the encapsulating platform automatically, it is characterized in that: the instrument controller (11), stepper motor driver (12), direct supply (13), pressure transducer Displaying Meter (14) and the platform base (15) that comprise motorized precision translation stage (1), push rod (2), pressure transducer (3), microsyringe support, microsyringe (5), capillary tube holder (6), threeway (7), capillary array (8), waste liquid tank (9), sample introduction conduit (10a), waste discharge conduit (10b), zone face plate; In platform base (15) one sides motorized precision translation stage (1), stepper motor and direct supply (13) are installed, the instrument controller of zone face plate (11) is connected with direct supply (13) with stepper motor driver (12), one end and the stepper motor of motorized precision translation stage (1), the other end of motorized precision translation stage (1) is connected with an end of push rod (2), and pressure transducer (3) is installed on the other end of push rod; At platform base (15) opposite side microsyringe support, capillary tube holder (6) and pressure transducer Displaying Meter (14) are installed; Be placed with microsyringe (5) on the microsyringe support, the tail end of microsyringe (5) can be close to pressure transducer (3), the head of microsyringe (5) links to each other with an end of sample introduction conduit (10a), and the other end of sample introduction conduit (10a) links to each other with first end of threeway (7); Be placed with capillary array (8) on the capillary tube holder (6), an end of capillary array (8) links to each other with second end of threeway (7), and the other end of capillary array (8) is water receiving tank behind capillary head base (6a); The 3rd end of threeway (7) links to each other with an end of waste discharge conduit (10b), and the other end of waste discharge conduit (10b) links to each other with waste liquid tank (9); Pressure transducer Displaying Meter (14) links to each other with pressure transducer (3) by circuit.
2. capillary array according to claim 1 cleans and the encapsulating platform automatically, and it is characterized in that: described microsyringe support (4) comprises end support (4a) and holder,head (4b); End support (4a) and holder,head (4b) are used for fixing microsyringe (5).
3. capillary array according to claim 1 cleans and the encapsulating platform automatically, it is characterized in that: described capillary tube holder (6), comprise that capillary head base (6a) and capillary midsection support (6b) are used for fixing capillary array (8), capillary midsection support (6b) is installed on the platform base (15), and adjustable height.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110073254 CN102253104B (en) | 2011-03-25 | 2011-03-25 | Automatic cleaning and glue pouring platform for capillary array |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110073254 CN102253104B (en) | 2011-03-25 | 2011-03-25 | Automatic cleaning and glue pouring platform for capillary array |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102253104A true CN102253104A (en) | 2011-11-23 |
| CN102253104B CN102253104B (en) | 2013-05-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110073254 Expired - Fee Related CN102253104B (en) | 2011-03-25 | 2011-03-25 | Automatic cleaning and glue pouring platform for capillary array |
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| Country | Link |
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| CN (1) | CN102253104B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108372083A (en) * | 2018-01-14 | 2018-08-07 | 贵州申科生物科技有限公司 | A kind of automatic glue injection apparatus for Capillary Electrophoresis |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3531241A1 (en) * | 1985-08-31 | 1987-03-05 | Eppendorf Geraetebau Netheler | Device for the controlled dispensing of liquids |
| CN2447787Y (en) * | 2000-08-11 | 2001-09-12 | 上海医大仪器厂 | Capillary cleaner |
| CN101750450A (en) * | 2008-12-17 | 2010-06-23 | 中国科学院大连化学物理研究所 | Automatic sampling device for array capillary electrophoresis |
| CN201543626U (en) * | 2009-11-06 | 2010-08-11 | 上海精密科学仪器有限公司 | Capillary tube cleaning device |
| CN201684754U (en) * | 2010-05-13 | 2010-12-29 | 福州大学 | Capillary tube automatic cleaner |
-
2011
- 2011-03-25 CN CN 201110073254 patent/CN102253104B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3531241A1 (en) * | 1985-08-31 | 1987-03-05 | Eppendorf Geraetebau Netheler | Device for the controlled dispensing of liquids |
| CN2447787Y (en) * | 2000-08-11 | 2001-09-12 | 上海医大仪器厂 | Capillary cleaner |
| CN101750450A (en) * | 2008-12-17 | 2010-06-23 | 中国科学院大连化学物理研究所 | Automatic sampling device for array capillary electrophoresis |
| CN201543626U (en) * | 2009-11-06 | 2010-08-11 | 上海精密科学仪器有限公司 | Capillary tube cleaning device |
| CN201684754U (en) * | 2010-05-13 | 2010-12-29 | 福州大学 | Capillary tube automatic cleaner |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108372083A (en) * | 2018-01-14 | 2018-08-07 | 贵州申科生物科技有限公司 | A kind of automatic glue injection apparatus for Capillary Electrophoresis |
| CN108372083B (en) * | 2018-01-14 | 2020-10-13 | 贵州申科生物科技有限公司 | Automatic glue injection device for capillary electrophoresis |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102253104B (en) | 2013-05-22 |
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