CN102253014A - System and method for surface plasmon resonance sensing detection - Google Patents
System and method for surface plasmon resonance sensing detection Download PDFInfo
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- CN102253014A CN102253014A CN 201110095470 CN201110095470A CN102253014A CN 102253014 A CN102253014 A CN 102253014A CN 201110095470 CN201110095470 CN 201110095470 CN 201110095470 A CN201110095470 A CN 201110095470A CN 102253014 A CN102253014 A CN 102253014A
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Abstract
The invention which is suitable for a photoelectric detection technology provides a system and a method for surface plasmon resonance sensing detection. The system comprises: light sources for generating sensing lights; a first lens for adjusting the sensing lights into collimated lights; a polarizer for obtaining P-polarized lights in the collimated lights; a prism for receiving the P-polarized lights to irradiate the P-polarized lights onto a sensing surface; a controller for controlling the light sources to allow the light sources to be sequentially lightened at different positions of a focal plane of the first lens and sequentially irradiate each P-polarized light onto the sensing surface from different angles; and an imaging mechanism for recording the intensity distribution of lights reflected by the sensing surface. According to the invention, the light sources are sequentially lightened at different positions of the focal plane of the first lens through an electronic control mode, so the P-polarized lights are sequentially irradiated onto the sensing surface from different angles, the intensity distribution of the lights reflected by the sensing surface is recorded in real time, SPR angular scan curves of each point on the sensing surface are obtained in parallel, the stability is good, and the speed is fast.
Description
Technical field
The invention belongs to photoelectric detecting technology, relate in particular to a kind of surface plasma resonance sensing detection system and method.
Background technology
Surface plasma body resonant vibration (Surface Plasmon Resonance, SPR) be a kind of emerging sensing technology, have high sensitivity, high flux, be easy to realize specific detection and real-time, and do not need advantages such as mark, be widely applied to industries such as biology, medicine, food quality safety, chemistry and environmental monitoring, particularly online in real time detect between DNA and the protein, between the protein molecule and biomolecule such as medicine-protein, nucleic acid-nucleic acid, Ag-Ab, receptor-ligand between interaction etc.But the SPR sensing technology all needs mechanical scanner that light source is carried out angular modulation at present, poor stability, and precision is low.
Summary of the invention
The purpose of the embodiment of the invention is to provide a kind of surface plasma body resonant vibration intensity detection system, and being intended to solve existing surface plasma body resonant vibration intensity detection system needs carry out angular modulation by mechanical scanner to light source, the problem of poor stability.
The embodiment of the invention is achieved in that a kind of surface plasma resonance sensing detection system comprises:
Light source is used to produce sense light;
First lens are used for described sense light is adjusted into collimated light;
The polarizer is used for obtaining the P polarized light of described collimated light;
Prism is used to receive described P polarized light, makes described P polarizing light irradiation in sensitive face;
Controller is used to control described light source, and described light source is lighted in regular turn in the focal plane of described first lens diverse location, and each P polarized light shines in described sensitive face with different angles in regular turn; And
Imaging mechanism is used to write down the light distribution through described sensitive face reflection.
Another purpose of the embodiment of the invention is to provide a kind of method that adopts above-mentioned surface plasma resonance sensing detection system to detect, and said method comprising the steps of:
Light source is lighted in regular turn in the focal plane of first lens diverse location, and each P polarized light shines in sensitive face with different angles in regular turn, writes down the light distribution through described sensitive face reflection in regular turn, the parallel SPR angle scanning curve that obtains each point on the described sensitive face; And
Be addressed to the optimum linear district of described SPR angle scanning curve, feed sample and carry out the spr signal record, real time record obtains the proterties of described sample through the light distribution of described sensitive face reflection.
The embodiment of the invention makes light source light in regular turn in the focal plane of lens diverse location by electronic control mode, the incident angle distribution is converted into light source space to distribute, thereby the P polarized light is shone in sensitive face in regular turn with different angles, real time record is through the light distribution of sensitive face reflection, the parallel SPR angle scanning curve that obtains each point on this sensitive face, this mode of obtaining SPR angle scanning curve is compared with the existing machinery scan mode, stability is good, and realized the multiple spot detection, improved detection speed and precision.
Description of drawings
Fig. 1 is the structural drawing of the surface plasma resonance sensing detection system that provides of first embodiment of the invention;
Fig. 2 is the structural drawing of the surface plasma resonance sensing detection system that provides of second embodiment of the invention;
Fig. 3 is the realization flow figure of the surface plasma resonance sensing detection method that provides of the embodiment of the invention;
Fig. 4 is a SPR angle scanning curve map.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer,, the present invention is further elaborated below in conjunction with drawings and Examples.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in qualification the present invention.
The embodiment of the invention makes light source light in regular turn in the focal plane of lens diverse location by electronic control mode, thereby the P polarized light is shone in sensitive face in regular turn with different angles, write down light distribution in regular turn through the sensitive face reflection, the parallel SPR angle scanning curve that obtains each point on this sensitive face, this mode of obtaining SPR angle scanning curve is compared with the existing machinery scan mode, stability is good, and speed is fast.
The surface plasma resonance sensing detection system that the embodiment of the invention provides comprises:
Light source is used to produce sense light;
First lens are used for described sense light is adjusted into collimated light;
The polarizer is used for obtaining the P polarized light of described collimated light;
Prism is used to receive described P polarized light, makes described P polarizing light irradiation in sensitive face;
Controller is used to control described light source, and described light source is lighted in regular turn in the focal plane of described first lens diverse location, and each P polarized light shines in described sensitive face with different angles in regular turn; And
Imaging mechanism is used to write down the light distribution through described sensitive face reflection.
The method that the above-mentioned surface plasma resonance sensing detection system of the employing that the embodiment of the invention provides detects may further comprise the steps:
Light source is lighted in regular turn in the focal plane of first lens diverse location, and each P polarized light shines in sensitive face with different angles in regular turn, writes down the light distribution through described sensitive face reflection in regular turn, the parallel SPR angle scanning curve that obtains each point on the described sensitive face; And
Be addressed to the optimum linear district of described SPR angle scanning curve, feed sample and carry out the spr signal record, real time record obtains the proterties of described sample through the light distribution of described sensitive face reflection.
Below in conjunction with specific embodiment realization of the present invention is described in detail.
Embodiment one
Fig. 1 shows the structure of the surface plasma resonance sensing detection system that the embodiment of the invention provides, and for convenience of explanation, only shows the part relevant with the embodiment of the invention.This surface plasma resonance sensing detection system comprises light source 1, first lens 2, the polarizer 3, prism 4, controller 5 and imaging mechanism.
The embodiment of the invention produces sense light by light source 1 earlier, and wherein sense light is preferably laser of narrowband, is generally produced by the laser instrument as light source.Wherein light source has a plurality ofly, exports the focal plane of first lens to for making each sense light, and the laser of narrowband that each light source produces is by optical fiber 7 transmission, thereby optical fiber has many, and multifiber constitutes intensive fiber array.The laser of narrowband Optical Fiber Transmission that suits, and be beneficial to fiber-optic output and form pointolite.
Then, the output terminal of each optical fiber is placed the focal plane of these first lens 2, each laser of narrowband becomes pointolite after optical fiber 7 outputs, and the light that this pointolite sends forms collimated lights through first lens 2.Obtain P polarized light in this collimated light by the polarizer 3, this P polarized light is from the plane of incidence incident of prism 4 and to shine in the sensitive face 40 of this prism 4 be the surface of metal-plated membrane, in order to realize surface plasma body resonant vibration.
Then, its focal plane diverse location in first lens 2 is lighted in regular turn, only need be lighted different light sources in regular turn herein and get final product by controller 5 control light sources 1.The light that each light source 1 sends all becomes collimated light in the focal plane diverse location of first lens 2 through first lens 2, each collimated light all becomes the P polarized light through the polarizer 3, and each collimates the P polarized light and all is radiated at the fixed sample district 41 that is positioned at sensitive face 40 with different angles from the plane of incidence incident of prism 4.
At last, write down the light distribution of reflecting in regular turn through sensitive face 40 by imaging mechanism, thus the parallel SPR angle scanning curve that obtains fixed sample district 41 each points on this sensitive face 40.Each collimates the P polarized light and shines at a certain angle in sensitive face 40, produce the SPR effect at the metal film place, promptly greater than that part of incident beam experiences total internal reflection of critical angle, and for one of them special angle, just in the time of satisfying the surface plasma body resonant vibration condition, the portion of energy of P polarized light is coupled into surface plasma wave, and energy of reflection light descends, minimum value appears in reflectivity, and this angle is called resonance angle.
Particularly, imaging mechanism comprises second lens 62, the 3rd lens 63 and the planar array detector of placing successively along optical axis 61, and wherein the back focal plane of second lens 62 overlaps with the front focal plane of the 3rd lens 63.Through the directional light of sensitive face 40 reflection is the i.e. front focal plane of the 3rd lens 63 of back focal plane that collimated light converges at second lens 62, and planar array detector 61 is positioned at the back focal plane of the 3rd lens 63.The corresponding different incidence angles of Different Light is whenever lighted a light source, planar array detector 61 (as CCD) record piece image, and the three is corresponding one by one, and this image has reflected the light distribution through sensitive face 40 reflections.Light Different Light in regular turn, planar array detector 61 writes down different images in regular turn.
Because planar array detector 61 has a plurality of pixels, a point in fixed sample district 41 on the corresponding sensitive face 40 of each pixel intensity.Therefore the pixel value of abstraction sequence image same position successively, the angle of drawing and pixel intensity curve promptly obtain on the sensitive face any SPR angle scanning curve.And every width of cloth image is made up of a plurality of pixels, all position pixel values of corresponding successively abstraction sequence image, and the angle of drawing respectively and pixel intensity curve, can walk abreast obtains the SPR angle scanning curve that fixed sample district 41 is had a few on the sensitive face 40.
As from the foregoing, the P polarized light of Different Light correspondence shines in sensitive face with different angles in the embodiment of the invention, only need to light Different Light in regular turn by controller and can walk abreast and obtain the SPR angle scanning curve of each point on the sensitive face, good than existing machinery scan mode stability, speed is fast.The embodiment of the invention is for improving the signal to noise ratio (S/N ratio) of imaging mechanism record reflective light intensity, establishes analyzer 9 between the exit facet of prism 4 and imaging mechanism.
Embodiment two
Fig. 2 shows the structure of the surface plasma resonance sensing detection system that the embodiment of the invention provides, and for convenience of explanation, only shows the part relevant with the embodiment of the invention.
Embodiment of the invention utilization scanning profile of optic fibre replaces a plurality of light sources among the embodiment one, i.e. in controller 7 every one steps of scanning of 10 control optical fiber, light source 1 is lighted once, and imaging mechanism generates piece image, is equivalent to switch to Different Light among the embodiment one.So only need a light source can finish the SPR angle scanning, greatly reduced cost.
Fig. 3 shows the realization flow of the surface plasma resonance sensing detection method that the embodiment of the invention provides, and for convenience of explanation, only shows the part relevant with the embodiment of the invention.
In step S101, light source is lighted in regular turn in the focal plane of first lens diverse location, each P polarized light shines in sensitive face with different angles in regular turn, writes down the light distribution through described sensitive face reflection in regular turn, the parallel SPR angle scanning curve that obtains each point on the described sensitive face;
In first embodiment of the invention, the laser of narrowband that controller 5 elder generations control first light source 11 sends is through 71 outputs of first optical fiber, first optical fiber, 71 output terminals are positioned at the focal plane of first lens 2, behind first lens 2, form collimated light, form the P polarized light through the polarizer 3, enter prism 4 from the plane of incidence of prism 4, with incident angle θ
1Shine the fixed sample district 41 on sensitive face 40, through the exit facet outgoing of the reflected light of sensitive face 40 reflection, through analyzer 9, second lens 62 and the 3rd lens 63, by planar array detector 61 record piece image F from prism 4
1Then, close first light source 11, light secondary light source 12, the laser of narrowband that secondary light source 12 sends is through 72 outputs of second optical fiber, the output terminal of second optical fiber 72 is positioned at the focal plane of first lens 2, forms collimated light behind first lens 2, forms the P polarized light through the polarizer 3, enter prism 4 from the plane of incidence of prism 4, with incident angle θ
2Shine fixed sample district 41, through the exit facet outgoing of the reflected light of sensitive face 40 reflection, through analyzer 9, second lens 62 and the 3rd lens 63, by planar array detector 61 record piece image F from prism 4 in sensitive face 40
2As above process, under the control of controller 5, light each light source in regular turn, write down the sensitive face image F under the different incidence angles θ situation in regular turn, be the corresponding incident angle θ of each width of cloth image F, the two is corresponding one by one, curve in the incident angle of drawing respectively θ and each image between the intensity level of same pixel, can walk abreast obtains the SPR angle scanning curve of each point on the sensitive face, as shown in Figure 4.
In second embodiment of the invention, the laser of narrowband that controller 10 control light sources 1 send is through optical fiber 7 outputs, and the output terminal of optical fiber 7 is positioned at the focal plane of first lens 2, behind first lens 2, form collimated light, form the P polarized lights through the polarizer 3, enter prism 4 from the plane of incidence of prism 4, with incident angle θ
1Shine the fixed sample district 41 on sensitive face 40, through the exit facet outgoing of the reflected light of sensitive face 40 reflection, through analyzer 9, second lens 62 and the 3rd lens 63, by planar array detector 61 record piece image F from prism 4
1Then, close light source 1, by 10 control optical fiber one steps of 7 scannings of controller, point bright light source 1, the laser of narrowband that light source 1 sends is through optical fiber 7 outputs, and the output terminal of optical fiber 7 is positioned at the focal plane of first lens 2, behind first lens 2, form collimated light, form the P polarized lights through the polarizer 3, enter prism 4 from the plane of incidence of prism 4, with incident angle θ
2Shine the fixed sample district 41 on sensitive face 40, through the exit facet outgoing of the reflected light of sensitive face 40 reflection, through analyzer 9, second lens 62 and the 3rd lens 63, by planar array detector 61 record piece image F from prism 4
2As above process, put bright light source 1 after scanning optical fiber 7 sections in regular turn, write down the sensitive face image F under the different incidence angles θ situation in regular turn, be the corresponding incident angle θ of each width of cloth image F, the two is corresponding one by one, curve in the incident angle of drawing respectively θ and each image between the intensity level of same pixel, can walk abreast equally obtains the SPR angle scanning curve of each point on the sensitive face as shown in Figure 4.
In step S102, be addressed to the optimum linear district of described SPR angle scanning curve, feed sample and carry out the spr signal record, real time record obtains the proterties of described sample through the light distribution of described sensitive face reflection.
As shown in Figure 4,, be addressed to the optimum linear district m of the SPR angle scanning curve of this laser of narrowband earlier, choose a certain incident angle θ among this optimum linear district m if the laser of narrowband wavelength is 850mm
mAnd and this incident angle θ
mCorresponding light source is lighted this light source.Feed sample 42 then and carry out the spr signal record, real time record is analyzed the proterties that obtains this sample 42 through the light distribution of sensitive face 40 reflections.
If the laser of narrowband wavelength is 630nn, be addressed to the optimum linear district n of the SPR angle scanning curve of this laser of narrowband earlier, choose a certain incident angle θ among this optimum linear district n
nAnd and this incident angle θ
nCorresponding light source is lighted this light source.Feed sample 42 then and carry out the spr signal record, real time record is analyzed the proterties that obtains this sample 42 through the light distribution of sensitive face 40 reflections.
The embodiment of the invention makes light source light in regular turn in the focal plane of lens diverse location by electronic control mode, the incident angle distribution is converted into light source space to distribute, thereby the P polarized light is shone in sensitive face in regular turn with different angles, real time record is through the light distribution of sensitive face reflection, the parallel SPR angle scanning curve that obtains each point on this sensitive face, this mode of obtaining SPR angle scanning curve is compared with the existing machinery scan mode, stability is good, and realized the multiple spot detection, improved detection speed and precision.Simultaneously, adopt optical fiber output sense light, only need the output terminal of multifiber is located at the focal plane of these lens, or the output terminal of this optical fiber is scanned get final product flexible operation, convenience on the focal plane of these lens.In addition, be to improve accuracy of detection, between the exit facet of prism and planar array detector, establish analyzer.
The above only is preferred embodiment of the present invention, not in order to restriction the present invention, all any modifications of being done within the spirit and principles in the present invention, is equal to and replaces and improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a surface plasma resonance sensing detection system is characterized in that, described system comprises:
Light source is used to produce sense light;
First lens are used for described sense light is adjusted into collimated light;
The polarizer is used for obtaining the P polarized light of described collimated light;
Prism is used to receive described P polarized light, makes described P polarizing light irradiation in sensitive face;
Controller is used to control described light source, and described light source is lighted in regular turn in the focal plane of described first lens diverse location, and each P polarized light shines in described sensitive face with different angles in regular turn; And
Imaging mechanism is used to write down the light distribution through described sensitive face reflection.
2. surface plasma resonance sensing detection system as claimed in claim 1 is characterized in that, described light source is a plurality of, and each light source is lighted under the control of described controller in regular turn.
3. surface plasma resonance sensing detection system as claimed in claim 1 is characterized in that, described light source is one, and described light source moves in the focal plane of described first lens under the control of described controller, and lights in regular turn.
4. as claim 2 or 3 described surface plasma resonance sensing detection systems, it is characterized in that described sense light becomes pointolite via optical fiber output, the output terminal of described optical fiber is positioned at the focal plane of described first lens.
5. surface plasma resonance sensing detection system as claimed in claim 4 is characterized in that described optical fiber has many, and multifiber constitutes intensive fiber array.
6. surface plasma resonance sensing detection system as claimed in claim 4 is characterized in that, described sense light is the laser of narrowband that is produced by laser instrument.
7. as claim 2 or 3 described surface plasma resonance sensing detection systems, it is characterized in that each P polarized light enters the fixed sample district of all shining behind the described prism in described sensitive face in regular turn.
8. surface plasma resonance sensing detection system as claimed in claim 1, it is characterized in that, described imaging mechanism comprises second lens, the 3rd lens and the planar array detector of placing successively along optical axis, the focal plane of described second lens overlaps with the focal plane of described the 3rd lens, directional light through described sensitive face reflection converges at described focal plane, and described planar array detector is positioned at another focal plane of described the 3rd lens.
9. surface plasma resonance sensing detection system as claimed in claim 8 is characterized in that, is provided with between the exit facet of described prism and the described planar array detector in order to improve the analyzer of signal to noise ratio (S/N ratio).
10. a method that adopts surface plasma resonance sensing detection system as claimed in claim 1 to detect is characterized in that, said method comprising the steps of:
Light source is lighted in regular turn in the focal plane of first lens diverse location, and each P polarized light shines in sensitive face with different angles in regular turn, writes down the light distribution through described sensitive face reflection in regular turn, the parallel SPR angle scanning curve that obtains each point on the described sensitive face; And
Be addressed to the optimum linear district of described SPR angle scanning curve, feed sample and carry out the spr signal record, real time record obtains the proterties of described sample through the light distribution of described sensitive face reflection.
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| CN102944537A (en) * | 2012-10-23 | 2013-02-27 | 深圳大学 | Detection system and detection method based on SPR |
| CN103389285A (en) * | 2012-05-09 | 2013-11-13 | 深圳大学 | Surface plasma resonance system and detection method thereof |
| CN103389284A (en) * | 2012-05-09 | 2013-11-13 | 深圳大学 | Surface plasma resonance system and detection method thereof |
| CN103454253A (en) * | 2013-06-25 | 2013-12-18 | 复旦大学 | Organic phosphorus detection method based on surface plasmon resonance |
| CN105486665A (en) * | 2016-01-26 | 2016-04-13 | 深圳大学 | SPR detection system and method |
| CN107655861A (en) * | 2017-11-08 | 2018-02-02 | 北京英柏生物科技有限公司 | Surface plasma resonance detector |
| CN108463712A (en) * | 2015-11-10 | 2018-08-28 | 拉克里赛恩斯有限责任公司 | System and method for determining sample osmotic pressure |
| CN108700513A (en) * | 2015-09-24 | 2018-10-23 | 拉克里赛恩斯有限责任公司 | Optical sensor, system and use its method |
| US10859493B2 (en) | 2015-06-12 | 2020-12-08 | Lacrisciences, Llc | Miniature, field portable, surface plasmon resonance apparatus and its applications in the detection of chemical and biological agents |
| CN114061801A (en) * | 2021-11-17 | 2022-02-18 | 重庆三峡学院 | Optical fiber V-groove cladding SPR strain sensor and manufacturing method thereof |
| CN116026760A (en) * | 2022-11-23 | 2023-04-28 | 广东工业大学 | Wavelength type SPR sensing system and method |
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| CN103389285A (en) * | 2012-05-09 | 2013-11-13 | 深圳大学 | Surface plasma resonance system and detection method thereof |
| CN103389284A (en) * | 2012-05-09 | 2013-11-13 | 深圳大学 | Surface plasma resonance system and detection method thereof |
| CN103389285B (en) * | 2012-05-09 | 2015-11-25 | 深圳大学 | Surface plasma resonance system and detection method thereof |
| CN103389284B (en) * | 2012-05-09 | 2016-03-02 | 深圳大学 | Surface plasma resonance system and its detection method |
| CN102944537B (en) * | 2012-10-23 | 2016-04-06 | 深圳大学 | A kind of detection system based on SPR and detection method thereof |
| CN102944537A (en) * | 2012-10-23 | 2013-02-27 | 深圳大学 | Detection system and detection method based on SPR |
| CN103454253A (en) * | 2013-06-25 | 2013-12-18 | 复旦大学 | Organic phosphorus detection method based on surface plasmon resonance |
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| US10859493B2 (en) | 2015-06-12 | 2020-12-08 | Lacrisciences, Llc | Miniature, field portable, surface plasmon resonance apparatus and its applications in the detection of chemical and biological agents |
| CN108700513A (en) * | 2015-09-24 | 2018-10-23 | 拉克里赛恩斯有限责任公司 | Optical sensor, system and use its method |
| US11406259B2 (en) | 2015-09-24 | 2022-08-09 | Lacrisciences, Llc | Optical sensors, systems and methods of using same |
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| CN108463712B (en) * | 2015-11-10 | 2021-11-02 | 拉克里赛恩斯有限责任公司 | System and method for determining osmolarity of a sample |
| CN108463712A (en) * | 2015-11-10 | 2018-08-28 | 拉克里赛恩斯有限责任公司 | System and method for determining sample osmotic pressure |
| US11016025B2 (en) | 2015-11-10 | 2021-05-25 | Lacrisciences, Llc | Systems and methods for determining sample osmolarity |
| US11650154B2 (en) | 2015-11-10 | 2023-05-16 | Lacrisciences, Llc | Systems and methods for determining sample osmolarity |
| CN105486665B (en) * | 2016-01-26 | 2018-07-31 | 深圳大学 | A kind of SPR detection methods |
| CN105486665A (en) * | 2016-01-26 | 2016-04-13 | 深圳大学 | SPR detection system and method |
| CN107655861A (en) * | 2017-11-08 | 2018-02-02 | 北京英柏生物科技有限公司 | Surface plasma resonance detector |
| CN114061801A (en) * | 2021-11-17 | 2022-02-18 | 重庆三峡学院 | Optical fiber V-groove cladding SPR strain sensor and manufacturing method thereof |
| CN114061801B (en) * | 2021-11-17 | 2023-09-26 | 重庆三峡学院 | Optical fiber V-groove type cladding SPR strain sensor and manufacturing method thereof |
| CN116026760A (en) * | 2022-11-23 | 2023-04-28 | 广东工业大学 | Wavelength type SPR sensing system and method |
| CN116026760B (en) * | 2022-11-23 | 2023-10-31 | 广东工业大学 | Wavelength type SPR sensing system and method |
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Application publication date: 20111123 |