CN102228207B - Method for processing wax apples and wax apple extract - Google Patents
Method for processing wax apples and wax apple extract Download PDFInfo
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Abstract
The invention relates to the field of food processing, in particular to a method for processing wax apples and a wax apple extract. The method comprises the following steps of: extracting from the wax apples by a biological enzymolysis technology, and ultra-filtering to obtain the wax apple extract, wherein the content of polysaccharide is 17.17 to 28.36mg/mL; and the content of polypeptide is 1.18 to 2.84mg/mL. The wax apple extract has good effects of treating lung dryness cough, bleeding hemorrhoids, dyspepsia, enteritis and dysentery. Quality inspection results of wax apple beverages prepared by using the wax apple extract show that the wax apple beverages all meet quality standard.
Description
Technical field
The present invention relates to food processing field, particularly the processing method of wax-apple and wax-apple extract.
Background technology
Wax-apple has another name called wax jambo, and formal name used at school Syzygium.samarangense (Bl.) Merr.et Perry is the tropical fruit (tree) of Myrtaceae Syzygium, and the juice multi-flavor is beautiful, sharp and clear sweetness, and the special fruit-like flavour of tool, and heat is low, the effect that having relieves summer heat quenches one's thirst.According to " China's book on Chinese herbal medicine " record, wax-apple is distinguished the flavor of sweet cold in nature, thoughts of returning home Liver Channel, purging intense heat and detonicating, eliminating dampness is antipruritic, dietotherapy effects such as also having appetizing, tasty and refreshing, diuresis, heat-clearing, moistening lung, cough-relieving, eliminating phlegm, cool blood, restrain and calm the nerves cures mainly the diseases such as wet mashed, the xeropulmonary cough of aphthae, thrush, sore, hiccup are more than, hemorrhoid hemorrhage, pipe abdomen fullness, enteritis and dysentery, diabetes.Grind into powder also with the fruit stone charcoal and can control traumatism and bleeding, the ulcer of lower limb.Simultaneously, the content of wax-apple tannin and folic acid is not low, can improve hypertension, and asthma is had good inhibition.In every 100g wax-apple pulp organization, moisture is about 90.75g, and total sugar content is about 7.680g, and protein content is about 0.690g, and Vc content is about 7.807mg, and organic acid is about 0.205mg/kg, and pericarp anthocyanidin is 0.073mg/kg, and nutriment is abundant.
But wax-apple is storage tolerance not, can only store a week under the general room temperature, and prosperous receipts are easily overstocked season, economic loss.There is not yet in the market the wax-apple deep processed product, the recovery rate of polysaccharide, polypeptide is lower in the wax-apple.
Summary of the invention
In view of this, the invention provides a kind of wax-apple processing method.The method utilizes biological enzymolysis technology to extract the wax-apple extract from wax-apple, has improved the content of polysaccharide, polypeptide nutrition composition in the wax-apple extract.And this wax-apple extract is made into the wax-apple beverage, can be prepared into medicine and/or the health products for the treatment of xeropulmonary cough, hemorrhoid hemorrhage, indigestion, enteritis and dysentery.
In order to realize the foregoing invention purpose, the invention provides following technical scheme:
The invention provides a kind of processing method of wax-apple, comprising:
Step 1: after wax-apple cleaning, peeling, section, at-20～-4 ℃ of lower freezing 1.5～2.5h, add the entry making beating, with 90～100 ℃ of hot bath blanching 10～15min, obtain the wax-apple homogenate;
Step 2: described wax-apple homogenate is cooled to 48～52 ℃, with acid for adjusting pH value to 3.3～3.7, adds pepsin or the pectase of 5～150,000 IU in the described wax-apple homogenate of every kg, enzymolysis 7.5～8.5h obtains the first enzymolysis liquid;
Step 3: described the first enzymolysis liquid is regulated pH value to 7.3～7.7 with alkali, under 48～52 ℃ of conditions, add the pancreatin of 4～100,000 IU in every kg raw material, enzymolysis 2.5～3.5h behind the enzyme-deactivating, obtains the second enzymolysis liquid;
Step 4: described the second enzymolysis liquid with acid for adjusting pH value to 5.8～6.2, after the Separation of Solid and Liquid, is collected parting liquid;
Step 5: the milipore filter that is 50～100KD with described parting liquid employing molecule interception carries out ultrafiltration, after collection filtrate concentrates distillation, obtains the wax-apple extract.
Pepsin is a kind of digestible protein enzyme, protein can be decomposed into peptone, and partially protein is broken down into the amino acid such as tyrosine, phenylalanine.Action pH value is: 1.5～5.0.
Pectase, a multienzyme complex of decompose pectin generally includes protopectinase, pectinesterase hydrolase, pectinesterase, by their synergy pectic substance is decomposed fully.Natural pectic substance changes into the pectin of water dissolvable under the protopectinase effect; Pectin is removed the methyl esters group by the catalysis of pectin methyl esters hydrolase, generates pectic acid; Pectic acid generates galacturonic acid through polygalacturonase class and the degraded of pectate lyase class.Pectase can be got through fermenting refining by aspergillus niger (Aspergillus niger), and decompose pectin is had good effect.Action pH value is: 2.5～6.0, and Optimun pH is: 3.5～4.5.
Pancreatin, be through extract, refining, the composite a kind of complex enzyme that forms, contain protease, lipase, amylase etc., have the abilities such as stronger decomposing protein and fat, be widely used in food, medical treatment, brewage, the industries such as silk, process hides.Processing method of jack-fruit provided by the invention, in the pancreatin of selection, in enzyme activity (IU), trypsase: amylopsin: pancreatic lipase=6: 70: 40.Preferably, in the method provided by the invention, in every 1g pancreatin, tryptic enzyme activity is 600～800IU, and the enzyme activity of amylopsin is 7000～9000IU, and the enzyme activity of pancreatic lipase is 4000～6000IU.
For adapt circumstance being provided for the first enzyme digestion reaction, before enzyme digestion reaction, need to regulate with acid the pH value of wax-apple homogenate.Acid described in step 2 of the present invention or the step 4 is selected from one or more the mixture in hydrochloric acid, phosphoric acid, citric acid or the sulfuric acid.
For adapt circumstance being provided for the second enzyme digestion reaction, before enzyme digestion reaction, need to regulate with alkali the pH value of the first enzymolysis liquid.Alkali described in the step 3 of the present invention is selected from one or more the mixture in NaOH, calcium hydroxide, potassium hydroxide, sodium acid carbonate or the ammoniacal liquor.
After enzyme digestion reaction finishes, need to carry out deactivation to enzyme, prevent enzyme digestion reaction continue carry out.The condition of enzyme-deactivating described in the step 3 of the present invention is at 95～100 ℃ of lower insulation 10～15min.Preferably, the condition of described enzyme-deactivating is for being preferably 13min 95～100 ℃ of lower insulations.
In order to make Separation of Solid and Liquid in the second enzymolysis liquid, to collect parting liquid and carry out subsequent treatment, Separation of Solid and Liquid described in the step 4 of the present invention comprises suction filtration, centrifugal, filtration step.
The present invention also provides the wax-apple that is made by above-mentioned processing method extract.
In addition, the present invention also provides the wax-apple beverage that contains the wax-apple extract that above-mentioned processing method makes.
Wax-apple beverage provided by the invention also comprises white granulated sugar, sweetener, citric acid and water.
As preferably, wax-apple beverage provided by the invention is calculated in mass percent, and contains described wax-apple extract 5.0～20.0%, white granulated sugar 3～5%, and sweetener 0.05～0.15%, citric acid 0.05～3.0%, surplus is water.
The present invention also provides the preparation method of wax-apple beverage, be calculated in mass percent, get 5.0～20.0% wax-apple extracts, add 3～5% white granulated sugars and 0.05～0.15% sweetener, add again entry, fully stir evenly, be the lemon acid for adjusting pH value to 4.5 of 20% (g/mL) again with volume fraction, the addition of citric acid is 0.05～3.0%, 123 ℃ of autoclaving 20min of wax-apple beverage gross weight, is cooled to room temperature and is the wax-apple beverage.
The invention provides a kind of wax-apple processing method, the method is utilized biological enzymolysis technology to extract the wax-apple extract from wax-apple and is made into the wax-apple beverage, has improved the content of polysaccharide, polypeptide nutrition composition in the wax-apple extract.Result of the test shows that in the wax-apple extract that the present invention makes, polyoses content is 17.17～28.36mg/mL, and content of peptides is 1.18～2.84mg/mL.The wax-apple extract that makes is 93.0% to xeropulmonary cough patient's total effective rate, and cure rate is 85.67%; Total effective rate to the hemorrhoid hemorrhage patient is 95.67%, and cure rate is 82.67%; Total effective rate to the indigestion patient is 95.33%, and cure rate is 87.66%; Total effective rate to the enteritis and dysentery patient is 93.33%, and cure rate is 88.0%.Comprehensive the above results, wax-apple extract provided by the invention has good therapeutic action to xeropulmonary cough, hemorrhoid hemorrhage, indigestion, enteritis and dysentery, can be for the preparation of medicine and/or the health products for the treatment of xeropulmonary cough, hemorrhoid hemorrhage, indigestion, enteritis and dysentery.Utilize biological enzymolysis technology that wax-apple is carried out deep processing, can make wax-apple keep the ecosystem of pollution-free food, the nutritional labeling of wax-apple is discharged be absorbed by the body, wax-apple is carried out deep processing can solve wax-apple because of the overstocked loss that causes.Simultaneously, wax-apple is carried out the added value that deep processing can well improve wax-apple, increase income farmers' income, have economic benefit and social benefit.
The present invention also provides the wax-apple beverage that is made by above-mentioned wax-apple extract.Wax-apple beverage Quality Detection is the result show, wax-apple beverage provided by the invention all meets quality standard.From national northwest, southwest, northeast, North China, the southeast, Central China's each age group, every profession and trade personage altogether among 1080 surveyees, be 90.03% to the sense of food satisfaction rate of wax-apple beverage provided by the invention, the local flavor satisfaction rate is 91.13%.Stability test is the result show, wax-apple beverage provided by the invention is after placing 12 months under the normal temperature stock condition, and every detection index all meets quality standard, shows that wax-apple beverage quality provided by the invention is stable.
The specific embodiment
The invention discloses a kind of wax-apple processing method, those skilled in the art can use for reference this paper content, suitably improve technological parameter and realize.Special needs to be pointed out is that all similarly replace and change apparent to those skilled in the art, they all are deemed to be included in the present invention.Method of the present invention and application are described by preferred embodiment, the related personnel obviously can change or suitably change and combination methods and applications as herein described within not breaking away from content of the present invention, spirit and scope, realizes and use the technology of the present invention.
Below in conjunction with embodiment, further set forth the present invention:
Embodiment 1 wax-apple processing method provided by the invention
The wax-apple of getting the 20.0kg maturation is cleaned, section, then puts into-4 ℃ refrigerator freezing 2.0h, takes out freezing wax-apple section, and the water that adds 80kg is pulled an oar, and with 98 ℃ of hot bath blanching 13min, cools off fast for subsequent usely, and 100.0kg weighs to get.Treat that homogenate is cooled to 50 ℃, be 4.5% hydrochloric acid adjust pH to 3.5 with mass fraction, the pepsic ratio that adds 100,000 IU in every kg homogenate, the pepsin that adds 1,000 ten thousand IU, after stirring evenly, keep pH value to 3.5,50 ℃ condition hydrolysis 8.0h, with the NaOH aqueous solution of 4.0g/mL with pepsin hydrolysis liquid adjust pH to 7.5, the ratio that adds the pancreatin of 40,000 IU in every kg slurries, the pancreatin that adds 4,000,000 IU, after stirring evenly, keep pH to 7.5,50 ℃ condition hydrolysis 3.0h, enzymolysis is heated to 98 ℃ after finishing, insulation 13min carries out enzyme-deactivating, being cooled to room temperature, is 4.5% hydrochloric acid adjust pH to 6.0 with mass fraction, and suction filtration gets supernatant 68.0kg, with the centrifuge residue of leaving away, collect supernatant and get 5.0kg, merge supernatant, altogether 73.0kg, carry out coarse filtration, collect filter liquor and get 72.0kg, filtering liquid carries out ultrafiltration with the filter membrane of average molecular interception 100KD, obtains filter liquor 68.0kg, concentrate with vacuum rotary evaporator, filter, get concentrate 48.0kg, be the wax-apple extract.
The wax-apple extract that makes is carried out determination of polysaccharide, polysaccharide molecule quantitative determination, polypeptide detection, determining content of peptides and acidity to be detected.Assay is as follows:
Polysaccharide is differentiated: phenolsulfuric acid reagent reacting (positive);
Polyoses content: 20.81mg/mL (phenolsulfuric acid method mensuration);
The polysaccharide molecule quantitative determination: the polysaccharide molecular weight of gained wax-apple extract is 32113 dalton (with reference to two appendix VH of pharmacopeia in 2005);
Polypeptide is differentiated: gets this product 1mL, adds ninhydrin solution number droplet, and heating, aobvious purple illustrates that this product contains the polypeptide composition;
Content of peptides: 1.61mg/mL (Forint phenol method);
The pH value is 4.9 (with reference to two appendix VH of pharmacopeia in 2005).
Embodiment 2 wax-apple processing methods provided by the invention
The wax-apple of getting the 20kg maturation is cleaned, section, then puts into-20 ℃ refrigerator freezing 1.5h, takes out freezing wax-apple section, and the water that adds 70.0kg is pulled an oar, and with 90 ℃ of hot bath blanching 15min, cools off fast for subsequent usely, and 90.0kg weighs to get.Treat that homogenate is cooled to 48 ℃, be 4.5% phosphoric acid adjust pH to 3.3 with mass fraction, the ratio that adds the pectase of 150,000 IU in every kg homogenate, the pectase that adds 1,350 ten thousand IU, after stirring evenly, keep pH value to 3.3,48 ℃ condition hydrolysis 8.5h, with the calcium hydroxide aqueous solution of 4.0g/mL with pectase hydrolyzate adjust pH to 7.3, the ratio that adds the pancreatin of 100,000 IU in every kg slurries, the pancreatin that adds 9,000,000 IU, after stirring evenly, keep pH to 7.3,48 ℃ condition hydrolysis 3.5h, enzymolysis is heated to 100 ℃ after finishing, insulation 10min carries out enzyme-deactivating, being cooled to room temperature, is 4.5% phosphoric acid adjust pH to 5.8 with mass fraction, and suction filtration gets supernatant 53.0kg, with the centrifuge residue of leaving away, collect supernatant and get 4.0kg, merge supernatant, altogether 57.0kg, carry out coarse filtration, collect filter liquor and get 54.0kg, filtering liquid carries out ultrafiltration with the filter membrane of average molecular interception 50KD, obtains filter liquor 52.0kg, concentrate with vacuum rotary evaporator, filter, get concentrate 38.0kg, be the wax-apple extract.
The wax-apple extract that makes is carried out determination of polysaccharide, polysaccharide molecule quantitative determination, polypeptide detection, determining content of peptides and acidity to be detected.Assay is as follows:
Polysaccharide is differentiated: phenolsulfuric acid reagent reacting (positive);
Polyoses content: 28.36mg/mL (phenolsulfuric acid method mensuration);
The polysaccharide molecule quantitative determination: the polysaccharide molecular weight of gained wax-apple extract is 34360 dalton (with reference to two appendix VH of pharmacopeia in 2005);
Polypeptide is differentiated: gets this product 1mL, adds ninhydrin solution number droplet, and heating, aobvious purple illustrates that this product contains the polypeptide composition;
Content of peptides: 2.84mg/mL (Forint phenol method);
The pH value is 5.0 (with reference to two appendix VH of pharmacopeia in 2005).
Embodiment 3 wax-apple processing methods provided by the invention
The wax-apple of getting the 20.0kg maturation is cleaned, section, then puts into-10 ℃ refrigerator freezing 2.5h, takes out freezing wax-apple section, and the water that adds 100.0kg is pulled an oar, and with 100 ℃ of hot bath blanching 10min, cools off fast for subsequent usely, and 120.0kg weighs to get.Treat that homogenate is cooled to 52 ℃, be 4.5% citric acid adjust pH to 3.7 with mass fraction, the pepsic ratio that adds 50,000 IU in every kg homogenate, the pepsin that adds 6,000,000 IU, after stirring evenly, keep pH value to 3.7,52 ℃ condition hydrolysis 7.5h, with the potassium hydroxide aqueous solution of 4.0g/mL with pepsin hydrolysis liquid adjust pH to 7.7, the ratio that adds the pancreatin of 70,000 IU in every kg slurries, the pancreatin that adds 8,400,000 IU, after stirring evenly, keep pH to 7.7,52 ℃ condition hydrolysis 2.5h, enzymolysis is heated to 95 ℃ after finishing, insulation 15min carries out enzyme-deactivating, being cooled to room temperature, is 4.5% citric acid adjust pH to 6.2 with mass fraction, and suction filtration gets supernatant 79.0kg, with the centrifuge residue of leaving away, collect supernatant and get 5.0kg, merge supernatant, altogether 84.0kg, carry out coarse filtration, collect filter liquor and get 81.0kg, filtering liquid carries out ultrafiltration with the filter membrane of average molecular interception 50KD, obtains filter liquor 78.0kg, concentrate with vacuum rotary evaporator, filter, get concentrate 56.0kg, be the wax-apple extract.
The wax-apple extract that makes is carried out determination of polysaccharide, polysaccharide molecule quantitative determination, polypeptide detection, determining content of peptides and acidity to be detected.Assay is as follows:
Polysaccharide is differentiated: phenolsulfuric acid reagent reacting (positive);
Polyoses content: 18.31mg/mL (phenolsulfuric acid method mensuration);
The polysaccharide molecule quantitative determination: the polysaccharide molecular weight of gained wax-apple extract is 38367 dalton (with reference to two appendix VH of pharmacopeia in 2005);
Polypeptide is differentiated: gets this product 1mL, adds ninhydrin solution number droplet, and heating, aobvious purple illustrates that this product contains the polypeptide composition;
Content of peptides: 1.21mg/mL (Forint phenol method);
The pH value is 4.8 (with reference to two appendix VH of pharmacopeia in 2005).
Embodiment 4 wax-apple processing methods provided by the invention
The wax-apple of getting the 20.0kg maturation is cleaned, section, then puts into-15 ℃ refrigerator freezing 1.8h, takes out freezing wax-apple section, and the water that adds 120.0kg is pulled an oar, and with 90 ℃ of hot bath blanching 12min, cools off fast for subsequent usely, and 140.0kg weighs to get.Treat that homogenate is cooled to 51 ℃, be 4.5% sulfuric acid adjust pH to 3.4 with mass fraction, the ratio that adds the pectase of 80,000 IU in every kg homogenate, the pectase that adds 1,120 ten thousand IU, after stirring evenly, keep pH value to 3.4,51 ℃ condition hydrolysis 8.2h, with the sodium bicarbonate aqueous solution of 4.0g/mL with pectase hydrolyzate adjust pH to 7.5, the ratio that adds the pancreatin of 50,000 IU in every kg homogenate, the pancreatin that adds 7,000,000 IU, after stirring evenly, keep pH to 7.4,51 ℃ condition hydrolysis 3.2h, enzymolysis is heated to 97 ℃ after finishing, insulation 11min carries out enzyme-deactivating, being cooled to room temperature, is 4.5% sulfuric acid adjust pH to 5.9 with mass fraction, and suction filtration gets supernatant 94.0kg, with the centrifuge residue of leaving away, collect supernatant and get 5.0kg, merge supernatant, altogether 99.0kg, carry out coarse filtration, collect filter liquor and get 96.0kg, filtering liquid carries out ultrafiltration with the filter membrane of average molecular interception 80KD, obtains filter liquor 91.0kg, concentrate with vacuum rotary evaporator, filter, get concentrate 65.0kg, be the wax-apple extract.
The wax-apple extract that makes is carried out determination of polysaccharide, polysaccharide molecule quantitative determination, polypeptide detection, determining content of peptides and acidity to be detected.Assay is as follows:
Polysaccharide is differentiated: phenolsulfuric acid reagent reacting (positive);
Polyoses content: 17.17mg/mL (phenolsulfuric acid method mensuration);
The polysaccharide molecule quantitative determination: the polysaccharide molecular weight of gained wax-apple extract is 31692 dalton (with reference to two appendix VH of pharmacopeia in 2005);
Polypeptide is differentiated: gets this product 1mL, adds ninhydrin solution number droplet, and heating, aobvious purple illustrates that this product contains the polypeptide composition;
Content of peptides: 1.18mg/mL (Forint phenol method);
The pH value is 4.8 (with reference to two appendix VH of pharmacopeia in 2005).
Embodiment 5 wax-apple processing methods provided by the invention
The wax-apple of getting the 20.0kg maturation is cleaned, section, then puts into-8 ℃ refrigerator freezing 2.2h, takes out freezing wax-apple section, and the water that adds 80.0kg is pulled an oar, and with 100 ℃ of hot bath blanching 15min, cools off fast for subsequent usely, and 100.0kg weighs to get.Treat that homogenate is cooled to 51 ℃, be 4.5% hydrochloric acid adjust pH to 3.6 with mass fraction, add the ratio of the pectase of 130,000 IU in every kg homogenate, add the pectase of 1,300 ten thousand IU, after stirring evenly, keep pH value to 3.5,50 ℃ condition hydrolysis 8.0h uses the ammoniacal liquor of 4.0g/mL with pectase hydrolyzate adjust pH to 7.5, adds the ratio of the pancreatin of 80,000 IU in every kg slurries, the pancreatin that adds 8,000,000 IU, after stirring evenly, keep pH to 7.5,5 ℃ condition hydrolysis 3.h, enzymolysis is heated to 97 ℃ after finishing, insulation 13min carries out enzyme-deactivating, being cooled to room temperature, is 4.5% hydrochloric acid adjust pH to 6.0 with mass fraction, and suction filtration gets supernatant 67.0kg, with the centrifuge residue of leaving away, collect supernatant and get 4.0kg, merge supernatant, altogether 71.0kg, carry out coarse filtration, collect filter liquor and get 68.0kg, filtering liquid carries out ultrafiltration with the filter membrane of average molecular interception 100KD, obtains filter liquor 66.0kg, concentrate with vacuum rotary evaporator, filter, get concentrate 46.0kg, be the wax-apple extract.
The wax-apple extract that makes is carried out determination of polysaccharide, polysaccharide molecule quantitative determination, polypeptide detection, determining content of peptides and acidity to be detected.Assay is as follows:
Polysaccharide is differentiated: phenolsulfuric acid reagent reacting (positive);
Polyoses content: 24.11mg/mL (phenolsulfuric acid method mensuration);
The polysaccharide molecule quantitative determination: the polysaccharide molecular weight of gained wax-apple extract is 35612 dalton (with reference to two appendix VH of pharmacopeia in 2005);
Polypeptide is differentiated: gets this product 1mL, adds ninhydrin solution number droplet, and heating, aobvious purple illustrates that this product contains the polypeptide composition;
Content of peptides: 2.41mg/mL (Forint phenol method);
The pH value is 5.0 (with reference to two appendix VH of pharmacopeia in 2005).
Embodiment 6 wax-apple beverages provided by the invention
Get the wax-apple extract 5.0kg among the embodiment 1, add 3.0kg white granulated sugar and 0.15kg sweetener, add again 79.65kg water (this moment, pol was 6.8 degree), fully stir evenly, use again the citric acid 0.20kg of 20% (g/mL), regulate pH value to 4.5, pour in the 350mL pop can, 123 ℃ of autoclaving 20min are cooled to room temperature and are the wax-apple beverage product, detect, the result is as follows:
Polysaccharide is differentiated: phenolsulfuric acid reagent reacting (positive);
Polyoses content: 1.07mg/mL (phenolsulfuric acid method mensuration);
Polypeptide is differentiated: gets this product 1mL, adds ninhydrin solution number droplet, and heating, aobvious purple illustrates that this product contains the polypeptide composition;
Content of peptides: 0.10mg/mL (Forint phenol method);
The pH value is 4.52 (with reference to two appendix VH of pharmacopeia in 2005).
Embodiment 7 wax-apple beverages provided by the invention
Get the wax-apple extract 10.0kg among the embodiment 2, add 4.0kg white granulated sugar and 0.10kg sweetener, add again 85.85kg water, fully stir evenly, use again the citric acid 0.05kg of 20% (g/mL) to regulate pH value to 4.5, pour in the 350mL pop can, 123 ℃ of autoclaving 20min, be cooled to room temperature and be the wax-apple beverage product, detect, the result is as follows:
Polysaccharide is differentiated: phenolsulfuric acid reagent reacting (positive);
Polyoses content: 2.54mg/mL (phenolsulfuric acid method mensuration);
Polypeptide is differentiated: gets this product 1mL, adds ninhydrin solution number droplet, and heating, aobvious purple illustrates that this product contains the polypeptide composition;
Content of peptides: 0.27mg/mL (Forint phenol method);
The pH value is 4.42 (with reference to two appendix VH of pharmacopeia in 2005).
Embodiment 8 wax-apple beverages provided by the invention
Get the wax-apple extract 15.0kg among the embodiment 3, add 5.0kg white granulated sugar and 0.10kg sweetener, add again 79.7kg water, fully stir evenly, use the citric acid 0.20kg of 20% (g/mL) to regulate pH value to 4.5,123 ℃ of autoclaving 20min are cooled to room temperature and are the wax-apple beverage product again, detect, the result is as follows:
Polysaccharide is differentiated: phenolsulfuric acid reagent reacting (positive);
Polyoses content: 2.61mg/mL (phenolsulfuric acid method mensuration);
Polypeptide is differentiated: gets this product 1mL, adds ninhydrin solution number droplet, and heating, aobvious purple illustrates that this product contains the polypeptide composition;
Content of peptides: 0.16mg/mL (Forint phenol method);
The pH value is 4.36 (with reference to two appendix VH of pharmacopeia in 2005).
Embodiment 9 wax-apple beverages provided by the invention
Get the wax-apple extract 20.0kg among the embodiment 4, add 5.0kg sugar and 0.05kg sweetener, add again 73.95kg water, fully stir evenly, use the citric acid 1.0kg of 20% (g/mL) to regulate pH value to 4.5,123 ℃ of autoclaving 20min are cooled to room temperature and are the wax-apple beverage product again, detect, the result is as follows:
Polysaccharide is differentiated: phenolsulfuric acid reagent reacting (positive);
Polyoses content: 3.31mg/mL (phenolsulfuric acid method mensuration);
Polypeptide is differentiated: gets this product 1mL, adds ninhydrin solution number droplet, and heating, aobvious purple illustrates that this product contains the polypeptide composition;
Content of peptides: 0.22mg/mL (Forint phenol method);
The pH value is 4.23 (with reference to two appendix VH of pharmacopeia in 2005).
Embodiment 10 wax-apple beverages provided by the invention
Get the wax-apple extract 15.0kg among the embodiment 5, add 3.0kg sugar and 0.15kg sweetener, add again 78.85kg water, fully stir evenly, use again the citric acid 3.0kg of 20% (g/mL) to regulate pH value to 4.5, pour in the 350mL pop can, 123 ℃ of autoclaving 20min, be cooled to room temperature and be the wax-apple beverage product, detect, the result is as follows:
Polysaccharide is differentiated: phenolsulfuric acid reagent reacting (positive);
Polyoses content: 3.57mg/mL (phenolsulfuric acid method mensuration);
Polypeptide is differentiated: gets this product 1mL, adds ninhydrin solution number droplet, and heating, aobvious purple illustrates that this product contains the polypeptide composition;
Content of peptides: 0.31mg/mL (Forint phenol method);
The pH value is 4.28 (with reference to two appendix VH of pharmacopeia in 2005).
The embodiment 11 wax-apple extract tests of pesticide effectiveness provided by the invention
The wax-apple extract that the embodiment of the invention 1 to 5 is provided carries out the test of pesticide effectiveness, the results are shown in Table 1.
Table 1 wax-apple extract test of pesticide effectiveness result
As shown in Table 1, the wax-apple extract that the embodiment of the invention 1 to 5 provides carries out the test of pesticide effectiveness to xeropulmonary cough, hemorrhoid hemorrhage, indigestion, each 300 patient of enteritis and dysentery, wherein, wax-apple extract provided by the invention is 93.0% to xeropulmonary cough patient's total effective rate, and cure rate is 85.67%; Total effective rate to the hemorrhoid hemorrhage patient is 95.67%, and cure rate is 82.67%; Total effective rate to the indigestion patient is 95.33%, and cure rate is 87.66%; Total effective rate to the enteritis and dysentery patient is 93.33%, and cure rate is 88.0%.
Comprehensive the above results, wax-apple extract provided by the invention has good therapeutic action to xeropulmonary cough, hemorrhoid hemorrhage, indigestion, enteritis and dysentery, can be for the preparation of medicine and/or the health products for the treatment of xeropulmonary cough, hemorrhoid hemorrhage, indigestion, enteritis and dysentery.
Embodiment 12 wax-apple beverage Quality Detection provided by the invention
The wax-apple beverage that the embodiment of the invention 6 to 10 is provided carries out Quality Detection, the results are shown in Table 2.
Table 2 wax-apple beverage Quality Detection result
Embodiment 13 wax-apple beverage officials provided by the invention can estimate
Choose at random respectively 1080 altogether of each age group, every profession and trade personages in national northwest, southwest, northeast, North China, the southeast, Central China, be divided into 36 groups according to age, engaged in trade, every group of 30 surveyees, the wax-apple beverage that the embodiment of the invention 6 to 10 is provided carries out sense of food and local flavor carries out the sense evaluation, the sense evaluation criterion sees Table 3, and evaluation result sees Table 4, table 5.
Table 3 wax-apple beverage official can evaluation criterion
The sense of food evaluation result of table 4 wax-apple beverage
The flavor evaluation result of table 5 wax-apple beverage
Shown by table 4, table 5 investigation result, from national northwest, southwest, northeast, North China, the southeast, Central China's each age group, every profession and trade personage altogether among 1080 surveyees, sense of food satisfaction rate to wax-apple beverage provided by the invention is 90.03%, and the local flavor satisfaction rate is 91.13%.
Embodiment 14 Detection of Stability
The wax-apple beverage that provides according to the embodiment of the invention 6 to 10 detects after placing 12 months under the normal temperature stock condition, be uniform transparent liquid, without macroscopic impurity, color and luster is for partially yellow or colourless, fragrant and sweet, clearly, has the distinctive delicate fragrance of wax-apple, without bad smell, soluble solid 2.4～2.8%, total acid content is 0.07～0.11g/100mL, and total arsenic is 0.08～0.12mg/L, tin, plumbous, the index such as copper and wax-apple beverage when just preparation has been dispatched from the factory without significant difference (P＞0.05), total plate count, Escherichia coli, mould, saccharomycete is 0cfu/mL, pathogenic bacteria (salmonella, Shigella, staphylococcus aureus) do not detect.Every detection index all meets statutory standards, illustrates that wax-apple beverage quality provided by the invention is stable.
The above only is preferred embodiment of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (9)
1. the processing method of a wax-apple is characterized in that, comprising:
Step 1: wax-apple at-20～-4 ℃ of lower freezing 1.5～2.5h, is added the entry making beating, with 90～100 ℃ of hot bath blanching 10～15min, obtain the wax-apple homogenate;
Step 2: described wax-apple homogenate is cooled to 48～52 ℃, with acid for adjusting pH value to 3.3～3.7, adds pepsin or the pectase of 5～150,000 IU in the described wax-apple homogenate of every kg, enzymolysis 7.5～8.5h obtains the first enzymolysis liquid;
Step 3: described the first enzymolysis liquid is regulated pH value to 7.3～7.7 with alkali, under 48～52 ℃ of conditions, add the pancreatin of 4～100,000 IU in every kg raw material, enzymolysis 2.5～3.5h behind the enzyme-deactivating, obtains the second enzymolysis liquid;
Step 4: described the second enzymolysis liquid with acid for adjusting pH value to 5.8～6.2, after the Separation of Solid and Liquid, is collected parting liquid;
Step 5: the milipore filter that is 50～100KD with described parting liquid employing molecule interception carries out ultrafiltration, after collection filtrate concentrates distillation, obtains the wax-apple extract.
2. processing method as claimed in claim 1 is characterized in that, acid described in step 2 or the step 4 is selected from one or more the mixture in hydrochloric acid, phosphoric acid, citric acid or the sulfuric acid.
3. processing method as claimed in claim 1 is characterized in that, alkali described in the step 3 is selected from one or more the mixture in NaOH, calcium hydroxide, potassium hydroxide, sodium acid carbonate or the ammoniacal liquor.
4. processing method as claimed in claim 1 is characterized in that, the condition of enzyme-deactivating described in the step 3 is at 95～100 ℃ of lower insulation 10～15min.
5. processing method as claimed in claim 1 is characterized in that, Separation of Solid and Liquid described in the step 4 comprises suction filtration, centrifugal and filtration step.
6. the wax-apple extract that makes such as each described processing method of claim 1 to 5.
7. the wax-apple beverage that contains wax-apple extract as claimed in claim 6.
8. wax-apple beverage as claimed in claim 7 also comprises white granulated sugar, sweetener, citric acid and water.
9. wax-apple beverage as claimed in claim 8 is characterized in that, is calculated in mass percent, and contains described wax-apple extract 5.0～20.0%, white granulated sugar 3～5%, and sweetener 0.05～0.15%, citric acid 0.05～3.0%, surplus is water.
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| CN102511619B (en) * | 2011-12-28 | 2013-05-15 | 福建省农业科学院 | Method for preparing syzygium samarangense preserved fruits |
| CN102669770A (en) * | 2012-05-31 | 2012-09-19 | 张松波 | Pineapple extract solution, preparation method thereof and pineapple drink containing extract solution |
| CN103351996A (en) * | 2013-07-12 | 2013-10-16 | 潘剑峰 | Production method for wax apple fruit vinegar |
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