CN102669770A - Pineapple extract solution, preparation method thereof and pineapple drink containing extract solution - Google Patents
Pineapple extract solution, preparation method thereof and pineapple drink containing extract solution Download PDFInfo
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- CN102669770A CN102669770A CN2012101770584A CN201210177058A CN102669770A CN 102669770 A CN102669770 A CN 102669770A CN 2012101770584 A CN2012101770584 A CN 2012101770584A CN 201210177058 A CN201210177058 A CN 201210177058A CN 102669770 A CN102669770 A CN 102669770A
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Abstract
The invention discloses a pineapple extract solution and a preparation method thereof. The method comprises the following steps of: 1) freezing a pineapple, adding water, pulping and then heating to the temperature of 90-100 DEG C to obtain pineapple pulp; 2) cooling the pineapple pulp, performing acid adjustment, and performing first enzymolysis; 3) performing second enzymolysis after alkali adjustment, and performing high-temperature enzyme killing; 4) performing the acid adjustment again, then performing solid-liquid separation, and collecting supernatant fluid; and 5) performing centrifugal separation and filtration on the supernatant fluid, and concentrating and distilling filtrate to obtain the pineapple extract solution. The method adopts a double-enzyme enzymolysis technology, so that macromolecular sugar and proteins in the pineapple can be fully hydrolyzed into micromolecular nutrient substances so as to be conductive to absorption by a human body. The invention further discloses a pineapple drink containing the pineapple extract solution, and the pineapple drink has the advantages of mellow taste, transparent color and luster and abundant nutrition.
Description
Technical field
The present invention relates to food processing field, the pineapple beverage that relates generally to a kind of pineapple extract and preparation method thereof and comprise this extract.
Background technology
Pineapple original name pineapple abounds with in China Fujian, Guangdong, Guangxi, Hainan and Taiwan and other places.Pineapple fruit is bigger, and is cylindrical, and pulp is faint yellow, the sweet acid of distinguishing the flavor of, and succulence has unique fruital.Pineapple is best in quality, nutritious, and its pulp contains a large amount of fructose, glucose, vitamin A, B, C, phosphorus, things such as citric acid and protease.Pineapple belongs to fruit in season, and storage time is shorter, and the pineapple goods then do not receive spatio-temporal restriction, but therefore long preservation comprises that the pineapple goods of pineapple beverage quite are received by the market.Publication number is the described natural pineapple juice beverage of the patent of invention of CN101467751A, and its processing method comprises: fresh pineapple is cleaned peeling, and after making beating squeezing, the filtration, the pineapple Normal juice that makes pure natural is subsequent use under germ-free condition; Get natural pineapple Normal juice again, add white sugar, salt, malic acid, vitamin C, after stirring at normal temperatures, add entry and mix and make natural pineapple juice beverage.The pineapple juice beverage color and luster that adopts this method to make is muddy, and local flavor is relatively poor.Publication number is that the preparation method of the described a kind of concentrated pineapple juice of patent of invention of CN102178297A comprises: 1) the pineapple peeling is broken into pulp; Be heated to 50-60 ℃; Amount with 100 kilograms of pineapple pulp 5-15g; Adding activity is the solid-state or liquid compound pectinase of 10000-30000 active unit/g, enzymolysis 40-80 minute, obtains enzymolysis pineapple slurry; 2) enzymolysis is good pineapple slurry sieves out fruit juice and pomace with the single track beater, and pomace squeezes with belt press, obtains squeezing also; Squeezing back gained pineapple pomace adds water and presses again once; The gained pressed liquor merges with the fruit juice that the first time, pressed liquor and single track beater sieved out, separates through centrifuge, obtains parting liquid; The pineapple pomace that centrifugation is come out squeezes once again, and pressed liquor and parting liquid merge; 3) the parting liquid vacuum concentrates, and obtains concentrate; 4) concentrate sterilization, cooling back sterile filling.This process using primary enzymolysis technology, enzymolysis is complete inadequately, though a kind of method slightly makes progress before causing concentrated pineapple juice color and luster, impression and mouthfeel, still has shortcoming.
Summary of the invention
The pineapple beverage that the technical problem that the present invention will solve provides a kind of pineapple extract and preparation method thereof and comprises this extract.The pineapple beverage color and luster for preparing is limpid, mouthfeel is pure just, aromatic flavour, be beneficial to human consumption and absorb.
Technical scheme provided by the invention is a kind of preparation method of pineapple extract, comprises following steps:
(1) get ripe pineapple peeling, section, freezing, making beating after, be heated to 90-100 ℃ and kept 10-15 minute, obtain the pineapple slurry;
(2) treat to be incubated when above-mentioned pineapple slurry is cooled to 40-50 ℃, to 3.5-4.5, the cellulase that adds 5-30 million international units/kg pineapple carries out the enzymolysis first time with acid for adjusting pH value, and enzymolysis time is 60-90 minute, obtains mixed liquor A;
(3) with alkali mixed liquor A is regulated pH value to 7.3-7.7, add the trypsase of 5-40 million international units/kg pineapple, carry out the enzymolysis second time; Hydrolysis temperature is 40-50 ℃, and enzymolysis time is 70-100 minute, and enzymolysis finishes the back high temperature enzyme that goes out; Naturally cool to room temperature again, obtain mixed liquid B;
(4) mixed liquid B is put into centrifuge and carry out Separation of Solid and Liquid, filter, collect supernatant;
(5) above-mentioned supernatant is carried out ultrafiltration with milipore filter, collect filtrating, filtrating is concentrated into the 60%-80% of its quality, be the pineapple extract.
In the above-mentioned steps (1), said freezing being meant placed the pineapple after the section under-4～0 ℃ of conditions freezing 1.5-2.5 hour.
In above-mentioned steps (2) and the step (4), described acid is citric acid, acetic acid, fumaric acid, malic acid or tartaric acid.
In the above-mentioned steps (3), described alkali is NaOH, potassium hydroxide, calcium hydroxide or sodium acid carbonate.
In the above-mentioned steps (5), the molecule interception of milipore filter is 50-100KD.
In the above-mentioned steps (1), the addition of water is 2-5 a times of pineapple quality.
The present invention also provides the pineapple that is obtained by method for preparing extract.
The present invention also provides the pineapple that comprises above-mentioned pineapple extract beverage.
In the above-mentioned pineapple beverage, the mass percent that the pineapple extract accounts for the pineapple beverage is 10%-20%.
Compared with prior art, the present invention to pineapple adopt freezing after heat treated again, can make the cell wall rupture of pineapple, nutrients mass-energy fully discharges, and makes that the pineapple extract nutrient is abundanter.Adopt zymolysis technique twice, can stodgy big molecular saccharides and protein fully be degraded into small-molecule substance, be beneficial to human consumption and absorb.The pineapple beverage color and luster for preparing is limpid, mouthfeel is pure just, aromatic flavour, nutritious, is fit to various drunk by people.
The specific embodiment
Be described further with embodiment below, but the present invention is not limited to these embodiment.
Embodiment 1 (pineapple extract)
1, gets 50kg pineapple peeling, section, put into refrigerator (4 ℃) freezing 1.5 hours, takes out the pineapple slice after freezing, put into beater in the lump with the water of 200kg and pull an oar, be heated to 90 ℃, be incubated 10 minutes, obtain pineapple and starch.
2, treat to be incubated when above-mentioned pineapple slurry is cooled to 40 ℃; Behind the winestone acid for adjusting pH value to 3.5 with 4% (w/w); In the ratio of every kg pineapple 5 million international units (than vigor) cellulase, add the cellulase of 250 million international units (than vigor), after stirring; Hydrolysis 60 minutes obtains mixed liquor A.
3, with the NaOH of 18g/mol mixed liquor A is regulated pH value to 7.3,, add the amylopsin of 250 million international units (than vigor) in the tryptic ratio of every kg pineapple 5 million international units (than vigor); After stirring; Enzymolysis is 70 minutes under 40 ℃ condition, and enzymolysis finishes back heat temperature raising to 100 ℃, be incubated 5 minutes and go out the enzyme processing; Reduce to room temperature, obtain mixed liquid B.
4, mixed liquid B is put into centrifuge and carry out Separation of Solid and Liquid, filter, collect supernatant;
5, use the molecule interception to carry out ultrafiltration above-mentioned parting liquid, collect filtrating and carry out vacuum in vacuum under for-0.096MPa, 60 ℃ condition and be concentrated into 60% of filtrating quality, promptly get the pineapple extract as the milipore filter of 50KD.
Embodiment 2 (pineapple extract)
1, gets 50kg pineapple peeling, section, put into refrigerator (3 ℃) freezing 2.5 hours, takes out the pineapple slice after freezing, put into beater in the lump with the water of 100kg and pull an oar, be heated to 100 ℃, be incubated 15 minutes, obtain pineapple and starch.
2, treat to be incubated when above-mentioned pineapple slurry is cooled to 50 ℃; Behind the lemon acid for adjusting pH value to 4.5 with 4% (w/w); In the ratio of every kg pineapple 10 million international units (than vigor) cellulase, add the cellulase of 500 million international units (than vigor), after stirring; Hydrolysis 90 minutes obtains mixed liquor A.
3, with the NaOH of 18g/mol mixed liquor A is regulated pH value to 7.7, starch the tryptic ratio of 20 million international units (than vigor), add the amylopsin of 1000 million international units (than vigor) in every kg pineapple; After stirring; Enzymolysis is 100 minutes under 50 ℃ condition, and enzymolysis finishes back heat temperature raising to 95 ℃, be incubated 10 minutes and go out the enzyme processing; Reduce to room temperature, obtain mixed liquid B.
4, mixed liquid B is put into centrifuge and carry out Separation of Solid and Liquid, filter, collect supernatant;
5, use the molecule interception to carry out ultrafiltration above-mentioned parting liquid, collect filtrating and carry out vacuum in vacuum under for-0.098MPa, 75 ℃ condition and be concentrated into 80% of filtrating quality, promptly get the pineapple extract as the 60KD milipore filter.
Embodiment 3 (pineapple extract)
1, gets 50kg pineapple peeling, section, put into refrigerator (2 ℃) freezing 2 hours, takes out the pineapple slice after freezing, put into beater in the lump with the water of 250kg and pull an oar, be heated to 100 ℃, be incubated 10 minutes, obtain pineapple and starch.
2, treat to be incubated when above-mentioned pineapple slurry is cooled to 45 ℃; Behind the lemon acid for adjusting pH value to 4.0 with 4% (w/w); In the ratio of every kg pineapple 30 million international units (than vigor) cellulase, add the cellulase of 1500 million international units (than vigor), after stirring; Hydrolysis 90 minutes obtains mixed liquor A.
3, with the NaOH of 18g/mol mixed liquor A is regulated pH value to 7.5, starch the tryptic ratio of 40 million international units (than vigor), add the amylopsin of 2000 million international units (than vigor) in every kg pineapple; After stirring; Enzymolysis is 80 minutes under 45 ℃ condition, and enzymolysis finishes back heat temperature raising to 95 ℃, be incubated 8 minutes and go out the enzyme processing; Reduce to room temperature, obtain mixed liquid B.
4, mixed liquid B is put into centrifuge and carry out Separation of Solid and Liquid, filter, collect supernatant;
5, use the molecule interception to carry out ultrafiltration above-mentioned parting liquid, collect filtrating and carry out vacuum in vacuum under for-0.096MPa, 70 ℃ condition and be concentrated into 70% of filtrating quality, promptly get the pineapple extract as the 70KD milipore filter.
Embodiment 4 (pineapple extract)
1, gets 50kg pineapple peeling, section, put into refrigerator (0 ℃) freezing 1.8 hours, takes out the pineapple slice after freezing, put into beater in the lump with the water of 150kg and pull an oar, be heated to 90 ℃, be incubated 15 minutes, obtain pineapple and starch.
2, treat to be incubated when above-mentioned pineapple slurry is cooled to 40 ℃; Behind the lemon acid for adjusting pH value to 3.5 with 4% (w/w); In the ratio of every kg pineapple 25 million international units (than vigor) cellulase, add the cellulase of 1250 million international units (than vigor), after stirring; Hydrolysis 60 minutes obtains mixed liquor A.
3, with the NaOH of 18g/mol mixed liquor A is regulated pH value to 7.4, starch the tryptic ratio of 35 million international units (than vigor), add the amylopsin of 1750 million international units (than vigor) in every kg pineapple; After stirring; Enzymolysis is 70 minutes under 40 ℃ condition, and enzymolysis finishes back heat temperature raising to 95 ℃, be incubated 5 minutes and go out the enzyme processing; Reduce to room temperature, obtain mixed liquid B.
4, mixed liquid B is put into centrifuge and carry out Separation of Solid and Liquid, filter, collect supernatant;
5, use the molecule interception to carry out ultrafiltration above-mentioned parting liquid, collect filtrating and carry out vacuum in vacuum under for-0.095MPa, 50 ℃ condition and be concentrated into 60% of filtrating quality, promptly get the pineapple extract as the 100KD milipore filter.
Embodiment 5 (pineapple beverage)
Get the pineapple extract 20kg that embodiment 1 makes; Add white sugar 10kg, salt 0.05kg, malic acid 0.05kg, vitamin C 0.005kg; The water that adds 70kg is again regulated PH to 5.5 with the tartaric acid of 4% (w/w), after stirring at normal temperatures; Through vacuum outgas, pasteurization, filling and sealing promptly gets transparent clarification, the visible impurity of no naked eyes, the positive pineapple beverage of mouthfeel alcohol.
Embodiment 6 (pineapple beverage)
Get the pineapple extract 15kg that embodiment 2 makes; Add white sugar 15kg, salt 0.05kg, malic acid 0.05kg, vitamin C 0.005kg; The water that adds 70kg is again regulated PH to 6.0 with the tartaric acid of 4% (w/w), after stirring at normal temperatures; Through vacuum outgas, pasteurization, filling and sealing promptly gets transparent clarification, the visible impurity of no naked eyes, the positive pineapple beverage of mouthfeel alcohol.
Embodiment 7 (pineapple beverage)
Get the pineapple extract 10kg that embodiment 3 makes; Add white sugar 20kg, salt 0.05kg, malic acid 0.05kg, vitamin C 0.005kg; The water that adds 70kg is again regulated PH to 5.8 with the tartaric acid of 4% (w/w), after stirring at normal temperatures; Through vacuum outgas, pasteurization, filling and sealing promptly gets transparent clarification, the visible impurity of no naked eyes, the positive pineapple beverage of mouthfeel alcohol.
Embodiment 8 (pineapple beverage)
Get the pineapple extract 25kg that embodiment 4 makes; Add white sugar 5kg, salt 0.05kg, malic acid 0.05kg, vitamin C 0.005kg; The water that adds 70kg is again regulated PH to 5.7 with the tartaric acid of 4% (w/w), after stirring at normal temperatures; Through vacuum outgas, pasteurization, filling and sealing promptly gets transparent clarification, the visible impurity of no naked eyes, the positive pineapple beverage of mouthfeel alcohol.
Claims (9)
1. the preparation method of a pineapple extract, it is characterized in that: this method comprises the steps:
(1) get ripe pineapple peeling, section, freezing, add the water making beating after, be heated to 90-100 ℃ and kept 10-15 minute, obtain the pineapple slurry;
Insulation when (2) treating that above-mentioned pineapple slurry is cooled to 40-50 ℃ to 3.5-4.5, is carried out first time enzymolysis by the ratio adding cellulase of 5-30 million international units/kg pineapple with acid for adjusting pH value, and enzymolysis time is 60-90 minute, obtains mixed liquor A;
(3) with alkali mixed liquor A is regulated pH value to 7.3-7.7; Ratio in 5-40 million international units/kg pineapple adds trypsase, carries out the enzymolysis second time, and hydrolysis temperature is 40-50 ℃; Enzymolysis time is 70-100 minute; Enzymolysis finishes the back high temperature enzyme that goes out, and naturally cools to room temperature again, obtains mixed liquid B;
(4) mixed liquid B is put into centrifuge and carry out Separation of Solid and Liquid, filter, collect supernatant;
(5) above-mentioned supernatant is carried out ultrafiltration with milipore filter, collect filtrating, filtrating is concentrated into the 60%-80% of its quality, be the pineapple extract.
2. the preparation method of pineapple extract according to claim 1, and being characterised in that: in the step (1), said freezing being meant placed the pineapple after the section under-4～0 ℃ of conditions freezing 1.5-2.5 hour.
3. the preparation method of pineapple extract according to claim 1, and be characterised in that: in the step (2), described acid is citric acid, acetic acid, fumaric acid, malic acid or tartaric acid.
4. the preparation method of pineapple extract according to claim 1, and be characterised in that: in the step (3), described alkali is NaOH, potassium hydroxide, calcium hydroxide or sodium acid carbonate.
5. the preparation method of pineapple extract according to claim 1, and be characterised in that: in the step (5), the molecule interception of milipore filter is 50-100KD.
6. the preparation method of pineapple extract according to claim 1, and be characterised in that: in the step (1), the addition of water is 2-5 a times of pineapple quality.
7. the pineapple extract that each said method prepares in the claim 1～6.
8. pineapple beverage, it contains the described pineapple extract of claim 7.
9. pineapple beverage according to claim 8 is characterized in that: the mass percent that the pineapple extract accounts for the pineapple beverage is 10%-30%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014186918A1 (en) * | 2013-05-23 | 2014-11-27 | 天宁香料（江苏）有限公司 | Flavoring pineapple juice extract liquid and application thereof |
| CN105249853A (en) * | 2015-08-28 | 2016-01-20 | 九阳股份有限公司 | Freeze wall breaking soybean milk making method of household soymilk machine and household soymilk machine |
| CN105936799A (en) * | 2016-06-06 | 2016-09-14 | 曹美兰 | Formaldehyde eliminating paint coating containing pineapple extracted solution |
| CN106962827A (en) * | 2017-04-18 | 2017-07-21 | 湖南文理学院 | A kind of protect liver snakeheaded fish nutrition oral formulations and its production method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101147611A (en) * | 2007-10-15 | 2008-03-26 | 广州南联实业有限公司 | Method for processing clearing type pineapple juice |
| CN101744324A (en) * | 2010-01-12 | 2010-06-23 | 东莞市美福乐商贸有限公司 | Method for producing clear pineapple juice |
| CN102228207A (en) * | 2011-06-23 | 2011-11-02 | 韩金光 | Method for processing wax apples and wax apple extract |
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2012
- 2012-05-31 CN CN2012101770584A patent/CN102669770A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147611A (en) * | 2007-10-15 | 2008-03-26 | 广州南联实业有限公司 | Method for processing clearing type pineapple juice |
| CN101744324A (en) * | 2010-01-12 | 2010-06-23 | 东莞市美福乐商贸有限公司 | Method for producing clear pineapple juice |
| CN102228207A (en) * | 2011-06-23 | 2011-11-02 | 韩金光 | Method for processing wax apples and wax apple extract |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014186918A1 (en) * | 2013-05-23 | 2014-11-27 | 天宁香料（江苏）有限公司 | Flavoring pineapple juice extract liquid and application thereof |
| CN105249853A (en) * | 2015-08-28 | 2016-01-20 | 九阳股份有限公司 | Freeze wall breaking soybean milk making method of household soymilk machine and household soymilk machine |
| CN105936799A (en) * | 2016-06-06 | 2016-09-14 | 曹美兰 | Formaldehyde eliminating paint coating containing pineapple extracted solution |
| CN106962827A (en) * | 2017-04-18 | 2017-07-21 | 湖南文理学院 | A kind of protect liver snakeheaded fish nutrition oral formulations and its production method |
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Application publication date: 20120919 |