CN102212470A - Radon gas and carbon monoxide gas united device for cell exposed contamination experiment - Google Patents
Radon gas and carbon monoxide gas united device for cell exposed contamination experiment Download PDFInfo
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- CN102212470A CN102212470A CN2011100928609A CN201110092860A CN102212470A CN 102212470 A CN102212470 A CN 102212470A CN 2011100928609 A CN2011100928609 A CN 2011100928609A CN 201110092860 A CN201110092860 A CN 201110092860A CN 102212470 A CN102212470 A CN 102212470A
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Abstract
The invention provides a radon gas and carbon monoxide gas united device for a cell exposed contamination experiment. A radon source capable of preparing radon gas, a predetermined amount of radon gas which is generated by a carbon monoxide generator and decay products thereof and the carbon monoxide gas are conveyed into a cell exposing room through an air inlet; a radon and carbon monoxide gas united detector is used for monitoring the content of the contaminated gas; a temperature control room is connected with the temperature control room to guarantee that the temperature of the experiment environment is constant; a cell culturing liquid supplying device is connected with a culture liquid room to provide nutrients for the normal growth of the cell; the upper surface of a cell membranous lamina is exposed to the cell exposing room to carry out complete contamination; the experimental waste gas is recovered through an air outlet and can be recycled; the whole device is sealed and has no leakage. The beneficial effects are as follows: the invention provides the device for the cell exposed contamination experiment, which does not use experimental animals and can randomly adjust the contents of the radon gas and the carbon monoxide gas and the contamination combination order; and the invention provides a new method and a new experimental installation in the following scientific research fields such as toxicology, molecular biology, cytology, radioactivity, medical science and the like.
Description
Technical field
The present invention relates to a kind of cell radon gas associating CO (carbon monoxide converter) gas and expose exposure experiment equipment.
Background technology
Carbon monoxide is colourless, tasteless, non-irritating gas, is the objectionable impurities of often meeting in the environment for human survival, and is very serious to the Health cost of human body; And the radon gas that is called as " stealthy killer " is the natural radioactivity rare gas element, it is the principal element of bringing out lung cancer, and the two harm to human body of uniting generation is subject to people's attention day by day, and is deep day by day to its research in fields such as toxicity, environmental protection, radiation, medical science.And the current experiments means lack accurate quantification control, easy to operate, safety do not have leak, intuitive and reliable simulation and make radon gas and CO (carbon monoxide converter) gas is united the specific equipment that exposes contamination, it is special that what lack is directly to carry out radon gas with the cell of experiment in vitro and CO (carbon monoxide converter) gas is united exposure exposure experiment equipment.Seriously restricted development of scientific research in this respect.
Summary of the invention
The purpose of this invention is to provide a kind of cell radon gas associating CO (carbon monoxide converter) gas and expose exposure experiment equipment, can not use laboratory animal, consume that radon is narrow-minded, easy to operate, safety do not have and leak, intuitive and reliable, accurate quantification control, can arbitrarily adjust the exposure experiment equipment that exposes of uniting of radon gas and carbon monoxide gas body burden and contamination built-up sequence.Opened up new method for field related science researchs such as toxicity, environmental protection, molecular biosciences, cell, radiation, medical science, new laboratory facilities are provided.
For achieving the above object, technical scheme of the present invention provides a kind of cell radon gas associating CO (carbon monoxide converter) gas exposure exposure experiment equipment.This equipment includes: can prepare the radon gas of the predetermined content that the radon need of radon gas and carbon monoxide producer produce and daughter thereof and two kinds of gases of carbon monoxide and be transported to the cellular exposure chamber through inlet mouth, the content of radon and carbon monoxide joint-detection instrument monitoring contamination gas; Temperature-controlling chamber connects temperature regulator to guarantee the experimental situation homo(io)thermism, and the cell culture fluid supply connects cultivates the nutrient that liquid chamber provides normal growth to need to cell; The upper surface of cell lamina membranacea is exposed to that cellular exposure is indoor fully contaminates; Experiment waste gas reclaims through the air outlet and can recycle once more; The totally-enclosed nothing of entire equipment is leaked.
The carbon monoxide gas generator of controlled content is one can produce the closed unit that continues with constant CO (carbon monoxide converter) gas, continues to enter into the indoor CO (carbon monoxide converter) gas exposure exposure experiment that carries out of cellular exposure with the CO (carbon monoxide converter) gas of constant level when opening carbon monoxide gas generator.Open or close the radon gas in the loop or the flow director of CO (carbon monoxide converter) gas respectively and can arbitrarily adjust contamination built-up sequence, carry out the separate cell radon gas and expose exposure experiment, separate cell CO (carbon monoxide converter) gas and expose exposure experiment and cell radon gas and CO (carbon monoxide converter) gas and unite and expose contamination.
Effect of the present invention is: provide a kind of cell radon gas associating CO (carbon monoxide converter) gas to expose exposure experiment equipment, can not use laboratory animal, consume that radon is narrow-minded, easy to operate, safety do not have and leak, intuitive and reliable, accurate quantification control, can arbitrarily adjust the exposure experiment equipment that exposes of uniting of radon gas and benzene volatilization gas content and contamination built-up sequence.Opened up new method for field related science researchs such as toxicity, environmental protection, molecular biosciences, cell, radiation, medical science, new laboratory facilities are provided.
Description of drawings
Accompanying drawing is the synoptic diagram of structure of the present invention;
Among the figure:
1, air pump
2, carbon monoxide producer
3, radon need
4, cellular exposure chamber
5, inlet mouth
6, air outlet
7, temperature-controlling chamber
8, cell lamina membranacea
9, cultivate liquid chamber
10, cell culture fluid supply
11, temperature regulator
12, radon and carbon monoxide joint-detection instrument
Embodiment
Reaching embodiment in conjunction with the accompanying drawings is illustrated cell radon gas associating CO (carbon monoxide converter) gas exposure exposure experiment equipment of the present invention.
Can prepare the radon gas of the predetermined content that the radon need 3 of radon gas and carbon monoxide producer 2 produce and daughter thereof and two kinds of gases of carbon monoxide and under the effect of air pump 1, be transported to cellular exposure chamber 4, the content of radon and carbon monoxide joint-detection instrument 12 monitoring radons and daughter and CO (carbon monoxide converter) gas through inlet mouth 5; The content that each corresponding flow director of adjusting can be adjusted cellular exposure chamber 4 interior radon gas and corresponding daughter and CO (carbon monoxide converter) gas reaches predetermined content.Temperature-controlling chamber 7 connects temperature regulator 11 to guarantee the experimental situation homo(io)thermism, and cell culture fluid supply 10 connects cultivates the nutrient that liquid chamber 9 provides normal growth to need to cell; The upper surface of cell lamina membranacea 8 is exposed in the cellular exposure chamber 4 and fully contaminates; To guarantee the normal physiologically active of cell, 6 recovery can recycle experiment waste gas once more through the air outlet in cultivating liquid chamber 9 in the lower surface infiltration; The totally-enclosed nothing of entire equipment is leaked.
The carbon monoxide producer 2 of controlled content is one can produce the closed unit that continues with constant CO (carbon monoxide converter) gas, and CO (carbon monoxide converter) gas lasting and constant level enters into and carries out CO (carbon monoxide converter) gas exposure exposure experiment in the cellular exposure chamber 4 when opening carbon monoxide producer 2.Open or close the radon gas in the pipeline or the flow director of CO (carbon monoxide converter) gas respectively and can arbitrarily adjust contamination built-up sequence, carry out the separate cell radon gas and expose exposure experiment, separate cell CO (carbon monoxide converter) gas and expose exposure experiment and cell radon gas and CO (carbon monoxide converter) gas and unite and expose contamination.
Embodiment:
When using cell radon gas associating CO (carbon monoxide converter) gas to expose exposure experiment equipment, at first close radon need 3 and carbon monoxide producer 2, open temperature regulator 11, open the cell lamina membranacea 8 that temperature-controlling chamber 7 will plant experimental cell and be placed in the cellular exposure chamber 4 closed temperature-controlling chamber 7.Open cell culture fluid supply 10 and radon need 3, air pump 1, carbon monoxide producer 2, open and adjust separately gas flow controller and expose separately or unite the exposure exposure experiment.When radon gas exposes contamination separately, only open radon need 3, air pump 1 drives radon gas and corresponding daughter is delivered in the cellular exposure chamber 4 through inlet mouth 5, regulate flow director and make the contamination gas flow in the cellular exposure chamber 4 constant, the upper surface that the radon gas of predetermined content and the mixed gas of corresponding daughter are evenly distributed on cell lamina membranacea 8 carries out the radon gas exposure experiment to experimental cell; The mixed gas of air outlet 6 recyclable radon gas and corresponding daughter recycles once more.If carry out CO (carbon monoxide converter) gas contamination then close radon need 3, open carbon monoxide producer 2 and corresponding gas flow controller, the CO (carbon monoxide converter) gas of predetermined content is input to carries out the CO (carbon monoxide converter) gas contamination in the cellular exposure chamber 4.Unite when exposing contamination: suitably regulate each corresponding flow controller as requested, can arbitrarily adjust radon gas and corresponding daughter thereof and carbon monoxide gas body burden and contamination built-up sequence is united the exposure exposure experiment.Experiment is closed radon need 3, air pump 1, carbon monoxide producer 2 and each flow director after finishing; Open temperature-controlling chamber 7 and take out the cell lamina membranacea 8 that the has experimental cell data analysis that experimentizes; Close cell culture fluid supply 10 and incubation controller 11 at last.
Claims (4)
1. a cell radon gas associating carbon monoxide exposes exposure experiment equipment, it is characterized in that: the CO (carbon monoxide converter) gas that the radon gas of radon need (3) preparation and daughter thereof and carbon monoxide producer (2) produce is by the cellular exposure chamber (4) of air pump (1) through inlet mouth (5) is delivered to temperature-controlling chamber (7) in, can adjust the content of radon gas in the cellular exposure chamber (4) and daughter and CO (carbon monoxide converter) gas and monitored by the radon in cellular exposure chamber (4) and carbon monoxide joint-detection instrument (12) by regulating flow director; (6) recovery can recycle mixed gas in the cellular exposure chamber (4) once more through the air outlet.
2. cell radon gas associating carbon monoxide according to claim 1 exposes exposure experiment equipment, it is characterized in that: temperature-controlling chamber (4) connects temperature regulator (11) and guarantees that the environment of cellular exposure chamber (4) and cultivation liquid chamber (9) is constant in helping the temperature of cells survival; Cellular exposure chamber (4) and cultivation liquid chamber (9) center fixed are placed cell lamina membranacea (8), there is a inlet mouth (5) to aim at cell lamina membranacea (8) upper surface in the cellular exposure chamber (4) and carries out sufficient radon gas exposure contamination or CO (carbon monoxide converter) gas exposure contamination to lower opening, cell lamina membranacea (8) lower surface soaks in cultivating liquid chamber (9), cultivates liquid chamber (9) and connects cell culture fluid supply (10) to guarantee the cell normal existence.
3. cell radon gas associating carbon monoxide according to claim 1 exposes exposure experiment equipment, it is characterized in that: the carbon monoxide gas generator of controlled content (2) is one can produce the closed unit that continues with constant CO (carbon monoxide converter) gas, and CO (carbon monoxide converter) gas lasting and constant level enters into and carries out CO (carbon monoxide converter) gas exposure exposure experiment in the cellular exposure chamber (4) when opening carbon monoxide gas generator (2).
4. cell radon gas associating carbon monoxide according to claim 1 exposes exposure experiment equipment, it is characterized in that: open or close the radon gas in the loop or the flow director of CO (carbon monoxide converter) gas respectively and can arbitrarily adjust contamination built-up sequence, carry out that the separate cell radon gas exposes exposure experiment, the separate cell CO (carbon monoxide converter) gas exposes exposure experiment and cell radon gas and CO (carbon monoxide converter) gas and unites the exposure exposure experiment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100928609A CN102212470A (en) | 2011-04-14 | 2011-04-14 | Radon gas and carbon monoxide gas united device for cell exposed contamination experiment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100928609A CN102212470A (en) | 2011-04-14 | 2011-04-14 | Radon gas and carbon monoxide gas united device for cell exposed contamination experiment |
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| CN102212470A true CN102212470A (en) | 2011-10-12 |
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| CN2011100928609A Pending CN102212470A (en) | 2011-04-14 | 2011-04-14 | Radon gas and carbon monoxide gas united device for cell exposed contamination experiment |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19526533A1 (en) * | 1995-07-20 | 1997-01-23 | Fraunhofer Ges Forschung | Nutritional medium delivered to cell culture through permeable membrane |
| CN102010818A (en) * | 2010-10-28 | 2011-04-13 | 天津开发区合普工贸有限公司 | Control device of cell exposing gas with controllable concentration |
| CN102010823A (en) * | 2010-10-28 | 2011-04-13 | 天津开发区合普工贸有限公司 | Cell culture solution flow supply device |
-
2011
- 2011-04-14 CN CN2011100928609A patent/CN102212470A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19526533A1 (en) * | 1995-07-20 | 1997-01-23 | Fraunhofer Ges Forschung | Nutritional medium delivered to cell culture through permeable membrane |
| CN102010818A (en) * | 2010-10-28 | 2011-04-13 | 天津开发区合普工贸有限公司 | Control device of cell exposing gas with controllable concentration |
| CN102010823A (en) * | 2010-10-28 | 2011-04-13 | 天津开发区合普工贸有限公司 | Cell culture solution flow supply device |
Non-Patent Citations (2)
| Title |
|---|
| 李建祥: "氡染毒的早期生物学效应", 《中国博士学位论文全文数据库 基础科技辑》, no. 4, 15 April 2010 (2010-04-15) * |
| 苗超: "氡及其子体和苯对小鼠血液和骨髓联合毒作用", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》, no. 5, 15 May 2006 (2006-05-15) * |
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Application publication date: 20111012 |