CN102210665B

CN102210665B - Method for preparing feed antibacterial agent by absorbing and grafting potassium diformate on epidesmine molecular screen

Info

Publication number: CN102210665B
Application number: CN2011100750235A
Authority: CN
Inventors: 陈南春; 汤泉; 张小虎; 王秀丽; 李子院; 单展展
Original assignee: Guilin University of Technology
Current assignee: Guilin University of Technology
Priority date: 2011-03-24
Filing date: 2011-03-24
Publication date: 2013-04-03
Anticipated expiration: 2031-03-24
Also published as: CN102210665A

Abstract

The invention discloses a method for preparing a feed antibacterial agent by absorbing and grafting potassium diformate on an epidesmine molecular screen. Regarding the defects that heavy metals in a silver zeolite antibacterial agent easily deposit in a body and cannot be applied to a feed, and the acidating agent potassium diformate cannot plays roles in intestinal tracts, the method takes the modified epidesmine as a carrier to adsorb the acidating agent potassium diformate so as to prepare the feed epidesmine antibacterial agent. Acidity detection and in vitro antibacterial tests indicate that: the feed epidesmine antibacterial agent has sustained release performance, has an excellent bacteriostasis effect to escherichia coli, and can prevent and treat diarrhea caused by the escherichia coli.

Description

Epidesmine molecular sieve adsorption grafting Potassium biformate prepares the method for feed antibacterial agent

Technical field

The molecular sieve that the present invention relates to the epidesmine preparation is carrier, and absorption grafting acidulant Potassium biformate prepares the method for feedstuff epidesmine antibacterial.

Background technology

Cause young animal's diarrhoeal diseases by escherichia coli, cause survival rate low (32%), solution is to add antibiotic in feedstuff at present.Along with pathogenic bacteria resistance to drugs increases, a large amount of uses of antibiotic are developed immunity to drugs to environment and are residual, and the harm humans and animals is healthy, and therefore, seeking minimizing antibiotic dosage method or Substitutes For Antibiotic becomes various countries' research emphasis.For reaching antibiotic purpose, be devoted to both at home and abroad the antibiotic property material is loaded on the various adsorbing materials, as utilize the silicate mineral cation exchange property, with Ag ⁺, Cu ²⁺, Zn ²⁺Deng or hexadecyl trimethylamine etc. be loaded on the silicate minerals such as bentonite, Montmorillonitum, zeolite by exchange reaction, make mineral tool antibacterial functions.Though heavy metal ion can sterilize, its easy deposition and be difficult for discharging in body after the desorbing from the carrier is therefore its application is limited to.

Potassium biformate is that its molecular formula is KCOOHHCOOH by a part formic acid and a part potassium formate dimer by hydrogen bond association.It is a kind of new antibiotic growth promoter substitute of developing in recent years, as feed additive, Potassium biformate is in gastric juice and intestinal fluid environment, can suppress escherichia coli and lactobacillus growth, and corresponding dry, OM, crude protein and essential amino acid and apparent ileal digestibility zero difference, only the apparent ileal digestibility of tyrosine slightly increases.The introducing of Potassium biformate has antibiotic growth promoting function to young animal's intestinal and gastric acidity and micro organism quantity, reduces anaerobe, lactobacillus, E.coli and yeast content in each section of digestive tract chyme, improves daily gain and feed intake; Promote the dry digestibility, thick protein increases the mineral absorptions such as phosphorus, potassium, calcium, magnesium, zinc, copper; An amount of Potassium biformate can improve young baby domestic animal fertility performance, reduces diarrhoea and mortality rate.But Potassium biformate absorbs very fast under one's belt, and its acidity Chang Wufa arrives small intestinal, can't reach the purpose that suppresses the harmful microorganism growth, promotes the probiotics growth, by the antibacterial preparation, and can delay time absorption and release in digestive system.

Summary of the invention

The objective of the invention is with epidesmine is raw material, by acidify, and hydrothermal crystallizing, absorption grafting, preparation feedstuff epidesmine antibacterial.

Concrete steps are:

(1) epidesmine is ground into the 180-200 order, epidesmine destroys its structure under the acid treatment of mass percent 13-15% salt, become amorphous amorphous state, as preparation zeolite molecular sieve presoma.In molal weight than acidify epidesmine: NaOH: NaAlO ₂: H ₂O=1: under 2: 1: 48 conditions, be first that 96% NaOH and mass concentration are 41% NaAlO with mass concentration ₂Be mixed with solution, join successively in the reactor, synthesis zeolite (Na ₂OAl ₂O ₃2SiO ₂4.5H ₂O), crystallization time 5.5-6 hour, crystallization temperature 90-100 ℃.The zeolite molecular sieve whiteness 95-97% of preparation, degree of crystallinity 98-99%, volume average particle size is 3-5 μ m, reaches the industry standard Q/Chalco A021-2004 that the absorption grafting agent is used molecular sieve.

(2) zeolite molecular sieve that makes take 2-2.5 gram step (1) is as carrier adsorption grafting Potassium biformate, the Potassium biformate mass concentration is 40-45mg/ml, response time is 3-3.2 hour, reaction temperature is room temperature, pH value is 6-6.5, and zeolite molecular sieve is 300-308mg/g to the saturated adsorption grafting amount of Potassium biformate; Acidity detects and shows, pH value reduces by 1.5, can improve the living environment of intestinal, makes the meta-alkalescence of intestinal be converted into slant acidity, the growth of suitable probiotics, and can prolong the sterilization timeliness; Escherichia coli, staphylococcus aureus are had obvious bacteriostasis property, and antibiotic rate reaches 63.6-65%.

The used epidesmine resource of the present invention mainly is distributed in Guangxi, and is larger, and its output condition is good, buries shallowly, is easy to reveal and adopts, and good exploitation prospect is arranged, but the Ore utilization rate is low, by reasonable development, takes full advantage of construction features, improves added value.By to molecular sieve, the interpretation of result of selection absorption grafting condition test, propose to pulverize---acidify---hydrothermal crystallizing---absorption grafting method for the problems referred to above.Use this preparation technology, can make antibacterial sterilization standard be used for the actual production operation.

Description of drawings

Fig. 1 is embodiment of the invention epidesmine molecular sieve diffracting spectrum.

Fig. 2 is the structural formula of embodiment of the invention Potassium biformate.

Fig. 3 is embodiment of the invention feedstuff epidesmine antibacterial SEM figure.

The specific embodiment

Embodiment:

(1) epidesmine is ground into 200 orders, epidesmine is to destroy its structure under the 15% salt acid treatment in mass percent, becomes amorphous amorphous state, as preparation zeolite molecular sieve presoma.In molal weight than acidify epidesmine: NaOH: NaAlO ₂: H ₂O=1: under 2: 1: 48 conditions, be first that 96% NaOH and mass concentration are 41% NaAlO with mass concentration ₂Be mixed with solution, join successively in the reactor, synthesis zeolite (Na ₂OAl ₂O ₃2SiO ₂4.5H ₂O), crystallization time 6 hours, 90 ℃ of crystallization temperatures.The zeolite molecular sieve whiteness 97% of preparation, degree of crystallinity 98%, volume average particle size are 3-5 μ m, reach the industry standard Q/Chalco A021-2004 that the absorption grafting agent is used molecular sieve.

(2) take 2 zeolite molecular sieves that make of gram steps (1) as carrier adsorption grafting Potassium biformate, the Potassium biformate mass concentration is 40mg/ml, and the response time is 3 hours, and reaction temperature is room temperature, pH value is 6.5, and zeolite molecular sieve is 302mg/g to the saturated adsorption grafting amount of Potassium biformate; Acidity detects and shows, pH value reduces by 1.5, can improve the living environment of intestinal, makes the meta-alkalescence of intestinal be converted into slant acidity, the growth of suitable probiotics, and can prolong the sterilization timeliness; Escherichia coli, staphylococcus aureus are had obvious bacteriostasis property, and antibiotic rate reaches 63.6%.

Claims

1. the preparation method of a feed antibacterial agent is characterized in that concrete steps are:

(1) epidesmine is ground into the 180-200 order, epidesmine destroys its structure under the acid treatment of mass percent 13-15% salt, become amorphous amorphous state, as preparation zeolite molecular sieve presoma; In molal weight than acidify epidesmine: NaOH: NaAlO ₂: H ₂O=1: under 2: 1: 48 conditions, be first that 96% NaOH and mass concentration are 41% NaAlO with mass concentration ₂Be mixed with solution, join successively in the reactor, synthesis zeolite Na ₂OAl ₂O ₃2SiO ₂4.5H ₂O, crystallization time 5.5-6 hour, crystallization temperature 90-100 ℃; The zeolite molecular sieve whiteness 95-97% of preparation, degree of crystallinity 98-99%, volume average particle size is 3-5 μ m;

(2) zeolite molecular sieve that makes take 2-2.5 gram step (1) is as carrier adsorption grafting Potassium biformate, the Potassium biformate mass concentration is 40-45mg/ml, response time is 3-3.2 hour, reaction temperature is room temperature, pH value is 6-6.5, and zeolite molecular sieve is 300-308mg/g to the saturated adsorption grafting amount of Potassium biformate.