CN102210454A - Microbial compound preparation and preparation method thereof - Google Patents
Microbial compound preparation and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a microbial compound preparation containing Lactobacillus acidophilus and a processing technology thereof. The microbial compound preparation contains a Lactobacillus acidophilus fermentation culture; the Lactobacillus acidophilus is taken as an active ingredient; and the viable count of the Lactobacillus acidophilus in the microbial compound preparation is more than 7.7*10<10>cfu/g, and the average particle size is less than 10mu m. The Lactobacillus acidophilus fermentation culture is obtained by culturing in a fermentation culture medium containing 3 to 5 percent of porous starch, 4 to 5 percent of defatted milk powder, 5 to 7 percent of defatted soybean powder, 4 to 6 percent of tapioca flour, 3 to 5 percent of papaya powder, 1 percent of glutamic acid soda, 1 to 2 percent of glucose, and 0.8 percent of table salt. Production processes are simple, and production cost is low. The microbial compound preparation can be prepared into medicinal preparations, health-care products, foods and nutritional supplements, can be taken as a core functional ingredient in feed additives, and has a broad application range.
Description
Technical field
The present invention relates to a kind of microbial compound preparation and preparation method thereof, specifically relate to a kind of microorganism formulation that contains lactobacillus acidophilus and preparation method thereof.Belong to microorganism field.
Background technology
Lactobacillus acidophilus (Lactobacillus acidophilus) is lactobacillus (Lactobacillus), Gram-positive, do not produce gemma, do not move, atrichia, with single, become the form of two or short chain to exist, fermentable sugars produces the anaerobism or the amphimicrobian microorganism of lactic acid, ethanol, hydrogen peroxide etc.G+C content is 36.0%-37.4%, optimal pH 5.5-6.0, and optimum growth temperature 35-38 ℃, not grow substantially below 20 ℃, poor heat resistance can utilize glucose, fructose, lactose, sucrose to carry out homofermentation, produces DL type lactic acid.Lactobacillus acidophilus is one of major microorganisms species in the humans and animals enteron aisle, be from baby's ight soil, to separate at first, being a kind of probio very important in the humans and animals gut flora, also is the probio of very paying attention to research and development in the present lactic acid bacteria family.Lactobacillus acidophilus is fermented in enteron aisle, produces lactic acid, acetic acid and antibiotic, has whole intestines effect, and intestines problems such as control and treatment chronic constipation, diarrhoea are had certain curative effect.In recent years, along with the research to lactobacillus acidophilus deepens continuously, lactobacillus acidophilus and metabolite thereof are proved healthy and helpful.It can improve or regulate gut flora balance, enhancing immunity of organisms, reduction cholesterol levels, alleviates lactose intolerance, prevention and treatment diarrhoea, reduces allergic reaction, prevent mycotic infection etc.Therefore, develop the microbial compound preparation that contains lactobacillus acidophilus, replenish the quantity of lactobacillus acidophilus,, improve immunity, promote healthy, significant for adjusting the human body intestinal canal microecological balance at human body intestinal canal by the external source mode.Be applied in the industries such as food, health products, medicine now.Find that also lactobacillus acidophilus has the effect that suppresses some growth of cancer cells, make the research of lactobacillus acidophilus and use more noticeable.
Contain the probiotics of lactobacillus acidophilus and the medicine various places of also being popular all over the world just gradually, calendar year 2001, in the probio list that can be used for health food that China Ministry of Public Health announces, lactobacillus acidophilus is listed.Live lactobacillus acidophilus preparation has been widely used in clinical treatment.Pro-Bionate and antibiotic are taken simultaneously, can obviously alleviate gastrointestinal side effect and correlation diarrhoea that oral broad-spectrum antibiotic causes, also can be used for treating trichomonas vaginitis.The mechanism that lactobacillus acidophilus suppresses the pathogen growth has: secretion antibacterial substance, occupancy protection, nutrient competition and promotion host immune function etc.In recent years, more abroad studies show that: lactobacillus acidophilus energy secreting bacteria element, growths such as strong inhibition shigella dysenteriae, gonococcus, Bacterium enteritidis, vagina flagellate; The culture supernatant of people lactobacillus acidophilus LB bacterial strain can obviously reduce the survival rate of helicobacter pylori (Helicobacterpylori) and to the ability of sticking of people's mucous membrane cell.
Abroad, carry out early for the research work of probios such as lactic acid bacteria, the product that contains probio is very general on Japan and European market, simultaneously, physiological function and the mechanism of action of probio is also compared comprehensive and deep research.The research of not restraining oneself aspects such as disease, inhibition diarrhoea, raising body's immunity, reduction cholesterol levels and the generation of inhibition tumour at the alleviation lactose of probio has all obtained certain progress.In nearest decades, the health promotion of probios such as relevant lactic acid bacteria acts on China and more and more is subject to people's attention.Though research of probio and service time are not long, its make fast progress and effect also clearly.
In recent years, the special physiological activity of probio is studied widely by various ways such as fermented dairy product and probioticses, and its research field progressively widened, and this series products increases just year by year.Though China is to the research comparative heat of probio, the research of lactobacillus acidophilus also had certain distance compared with developed countries.Therefore, the research and development probiotic products has the bigger market space, will have very important significance to revitalizing industries such as China's food, feed, medicine and health products.
At present, the produce market of probio that contains lactobacillus acidophilus is very active, and kind is more and more.In food service industry, comprise dairy products, meat products and the fruit and vegetable product etc. of probiotics fermention; Medicine and health products trade all contain different types of probio as whole intestines life, beautiful pearl Chang Le, ANGLI No.1 etc., and throw in various ways such as capsule, tablet, pulvis and oral liquids.Yet can the stability of probio be directly connected to product and bring into play its health promotion effect in the product, also is one of key index of examining the quality of the production.
Studies show that in order to reach desirable health promotion effect, in the product based on probios such as lactobacillus acidophilus, number of viable should remain on 10
6More than the cfu/mL.Therefore, in the processing of probio based article, storage and transport process, should take measures as far as possible the to reduce inactivation of probio.
The research and development of lactobacillus acidophilus more and more get more and more people's extensive concerning.
Because lactobacillus acidophilus is with health role, the research about this respect in recent years gets more and more, and has now become the research focus of active bacteria formulation.Active bacteria formulation should be kept higher viable bacteria level before consumption, and current many products all can not prevent the decay of viable count effectively, caused product viable bacteria preservation term short.Be unfavorable for the transportation and the sale of product.Improve and contain the viable bacteria content of lactobacillus acidophilus goods and prolong the viable bacteria preservation term, become the key problem in technology in the live lactobacillus acidophilus goods R﹠D process.
Traditional medicine and food manufacturing drying means mainly contain freeze-drying and vacuum freeze-drying method, the former carries out dry method under-20 ℃～-160 ℃ low-temperature condition, the latter then is at normal temperatures, to carry out dry method under the pressure state of 50～400Pa.Freeze drying is that microbial cell is freezing at low temperature environment, keeps the certain vacuum of environment then.Make the ice distillation, reach dry at last.Microorganism will be in resting state under the condition of low temperature drying, and metabolism weakens relatively, be beneficial to the long preservation activity.The cryogenic freezing of freezing dry process and process of vacuum drying can make microbial cell change.Unsuitable freeze drying is handled and can be reduced activation of microorganism.Therefore in order to protect the microbial product activity; usually before freeze-drying, add some the material of defencive function is arranged; physics when these materials can change the biological sample freeze drying, chemical environment; alleviate or the infringement of cell when preventing freeze drying or rehydration, keep original various physio-biochemical characteristics and biologically active as far as possible.But nearly all bacterium generally can both survive under cryogenic conditions, has increased the pollution probability of assorted bacterium greatly, can cause serious prestige evil to the purpose flora, and then reduces the viable count and the performance of purpose flora.Simultaneously, freeze-drying equipment needed thereby costliness, complicated operation, the cycle is long, is not suitable for suitability for industrialized production.The spray drying process that also has high temperature in addition, but, also can kill the purpose flora because higher temperature not only can be killed assorted bacterium.So, how to make the purpose flora be issued to bigger value-added effect in situation about not being bacterial contamination, improve its activity, amplify its performance, be the particularly problem demanding prompt solution that faces jointly of suitability for industrialized production of present microorganism application.
Summary of the invention
The objective of the invention is to select suitable fermentation medium and processing technology a kind of function be many to provide, number of viable height in the preparation, the microbial compound preparation of finished product long shelf-life, described preparation is active component with the lactobacillus acidophilus, and its viable count is greater than 7.7 * 10
10Cfu/g, average grain diameter is less than 10 μ m.
Preparation viable count of the present invention is very high, safe, and the function uniqueness has enhancing immunity, regulates gut flora, functions such as reducing blood lipid, reduction fat content.
Bacterium powder of the present invention is superfine micron-sized powder, as the pulvis steady quality.Good mouthfeel also has the special dietary material that papaya powder and tapioca starch etc. provide.
Preparation of the present invention can be prepared into various products, comprises pharmaceutical preparation, health products, food, nutritious supplementary pharmaceutical and the feed etc. that comprise described preparation.
The purposes of preparation of the present invention in pharmaceutical formulations, health products, food, nutritious supplementary pharmaceutical and feed etc.
Owing to the double protection of described preparation by porous-starch and carbohydrate, its acid resistance, high temperature resistant and good salt tolerance, but long preservation is used.The adding of porous-starch can make the bacterial classification embedding wherein, has reduced thalline and has contacted with the degree of depth of hot-air and chamber wall; The application of carbohydrate can make the thalline in the starch hole be in the airtight relatively environment, not only reduced the influence that causes because of being heated, can also well protect thalline because of installing operating noise and other extraneous factors to its influence that causes, thereby the quantity that makes thalline in the bacterium powder can guarantee to reach the maximization of effect a higher level; Adding papaya powder and tapioca starch is because they are rich in the various special nutriment of C, N and vitamin and himself existence, the necessary various nutrition of thalli growth have been guaranteed fully, make the growth that flora in the mixture can be healthy, thereby, can keep the one-tenth live time and the survival rate of bacterial classification to increase greatly, thereby make this product can long preservation.Low, the various problems of utilizing manufacturing process of the present invention to can be good at solving to run in the traditional mode of production process such as afunction is serious, quality instability of living bacterium rate, use better simply production equipment, efficient, stable continuous is produced purity height, the microorganism formulation that contains lactobacillus acidophilus that living bacterium rate is high.
The present inventor shows through experimental study, streptococcus thermophilus has facilitation to lactobacillus acidophilus, for increasing the content of lactobacillus acidophilus, can in shaking bottle cultivation and fermented and cultured, add an amount of streptococcus thermophilus, wherein the ratio of streptococcus thermophilus and lactobacillus acidophilus is 0.1-1: 1.Preparation technology is constant.
The processing technology of microbial compound preparation of the present invention mainly comprises: steps such as the seed culture of lactobacillus acidophilus, the fermented and cultured of lactobacillus acidophilus and spray-drying, and concrete technology is as follows:
One, culture of strains and preparation
1, the preparation of culture medium
(1) culture medium of seed culture: MRS culture medium, after the packing 120 ℃, the 30min sterilization, the concrete composition of MRS culture medium: peptone 10g, yeast extract 5g, beef extract 10g, glucose 20g, sodium acetate 5g, ammonium citrate 2.15g, Tween80 1ml, MgSO
47H
2O 0.58g, MnSO
44H
2O0.05g, K2HPO
42g, agar powder 17g (fluid nutrient medium does not add), water 1000ml.
(2) fermentation medium: porous-starch 3%-5%, skimmed milk power 4%-5%, defatted soy flour 5%-7%, tapioca starch 4%-6%, papaya powder 3%-5%, glutamic acid soda 1%, glucose 1%-2%, salt 0.8%, above-mentioned content is in mass volume ratio, adding distil water 100%.Wherein, tapioca starch/papaya powder obtains by cassava/pawpaw is pulverized the back.
2, bacterial classification is cultivated
(1) inclined-plane is cultivated: get the lactobacillus acidophilus bacterial classification and cultivated 48 hours for 37 ℃ in slant medium; Test under microscope should meet the standard type of lactobacillus acidophilus after cultivating, and it is good to grow.
(2) shaking bottle cultivates: get the inoculation of lactobacillus acidophilus slant culture and shake in bottle fluid nutrient medium and cultivate, cultivated 48 hours for 37 ℃;
(3) seed culture:
The lactobacillus acidophilus shake-flask seed is to be inoculated in the first class seed pot under the 1% ratio aseptic condition, be cultured to the thalline logarithmic growth and (cultivate 48h latter stage, 37 ℃ of temperature), be seeded in the secondary seed jar with 10% ratio under the aseptic condition, the secondary seed jar is cultured to thalline logarithmic growth latter stage (cultivating 48h, 37 ℃ of temperature).The culture medium of first class seed pot is the MRS culture medium, the same fermentation medium of the culture medium of secondary seed jar.
3, fermentation tank culture
The nutrient solution that obtains in the seed culture is inoculated in fermentation medium with 1-5%, and mixing, condition of culture are 37 ± 1 ℃ of temperature, and pressure is 0.02-0.04Mpa, stir: 5 minutes/3 hours, continuously fermented 48 hours.Record fermentation temperature, pressure, mixing time and situations such as number of times, pH, incubation time and zymotic fluid metabolism pollution.Microscopy lactobacillus acidophilus form is normal, does not have assorted bacterium, stops fermentation.Be made into muddy with agitator or blender, and filter.
4, spray-drying (powder process)
With 120 ℃-150 ℃ of inlet temperatures, outlet temperature is that 65 ℃-80 ℃ spray dryer carries out spray-drying, and the production average grain diameter is the microorganism formulation that contains lactobacillus acidophilus of the powdery of 10 μ m.
5, packing and packing
On the vacuum racking machine, carry out packing on request, decals, warehouse-in, stored refrigerated.
The present invention makes dry bacterium powder through processing at last, its spray-drying installation, start the back spray droplet and in casing, form column vortex wind, avoided contacting of bacterium powder and tank wall, and pass through fast cooling behind the hyperthermia drying at short notice, like this, just guaranteed the high survival rate of bacterium powder in the process of high temperature drying.In addition, because thalline has been protected in the reasonable use of porous-starch and carbohydrate fully, further prevented the damage of thalline.
Prescription and the preparation processing technology of the present invention by optimizing fermentation medium makes that viable bacteria content height, the moisture in the preparation is low, has benefits such as stomach juice-resistant, bile tolerance and gives birth to function.The viable bacteria content of microorganism formulation of the present invention is very high, and it is multiple functional, nutritional sufficiency, and be superfine micron-sized powder, can be made into pharmaceutical preparation, be used to regulate gut flora, reduce blood fat and regulate immunity, also can be made into additive and add in food and the health products replenishers as healthy food and nutriment, can also be made into feed addictive in addition and add feed, can improve food utilization efficiency, increase meat laying hen output, reduce the fat content of animal, promote growth of animal, improve immunity and premunition, thereby improve the animal product quality.
Description of drawings
Fig. 1: preparation technology's flow chart of microbial compound preparation of the present invention
The specific embodiment
Embodiment 1:
Embodiment 2:
Embodiment 3:
Embodiment 4:
Embodiment 5:
Embodiment 6; Contrast experiment's group
Under the situation of other components unchanged in the fermentation medium (bacteria culture fluid 2.5%, skimmed milk power 4.5%, defatted soy flour 6.0%, glutamic acid soda 1.0%, salt 0.8%), the interpolation of contrast porous-starch, papaya powder, tapioca starch and glucose is to the influence of last viable count.
Embodiment 7: detect the viable bacteria level of microbial compound preparation of the present invention after stored refrigerated
Detect microbial compound preparation that the fermentative medium formula utilize embodiment 1-5 produces respectively before 4 ℃ of preservations and the viable bacteria level of 4 ℃ of preservations after 1 month, 3 months, 6 months, 12 months, 24 months.The data that record are as follows:
As can be seen from the above table, utilize the dry bacterium powder of technology preparation of the present invention can obtain higher bacterial classification survival rate, and preservation term is longer, survival rate is difficult for reducing.
Embodiment 8: microbial compound preparation of the present invention can be regulated gut flora
The microbial compound preparation that utilizes embodiment 1 described fermentation medium to be obtained detects its influence to gut flora.
(1) mouse experiment
1, animal grouping and modelling
The human body recommended amounts of compound formulation of the present invention is 1g/50Kg every day, and basic, normal, high dosage group dosage is respectively 0.02g/Kg, 0.2g/Kg, 0.6g/Kg, is equivalent to 1,10,30 times of human body recommended amounts respectively.Other establishes the 0.9%NaCl control group.
Get 40 of male Sprague-Dawley rats, be divided into 4 groups at random, 10 every group.
2, gut flora analysis
Force method gather rat fresh excreta 0.5-1.0g, place and weigh in vitro and weigh once more to determine example weight.Sample is placed the aseptic triangular flask of the band bead that is added with dilution in advance, fully shake 20min it is homogenized, again with the capable 10 times of continuous serieses dilution of dilution; Select suitable dilution factor to get 0.1ml and be inoculated in respectively in enterobacteria, enterococcus, lactobacillus and the Bifidobacterium selective medium, 3 flat boards of each dilution factor inoculation; 37 ℃ of aerobic cultivation 18-24h of the inoculation preceding two kinds of culture mediums in back, 37 ℃ of anaerobism of back two kinds of culture mediums are cultivated 48-72h.Cultivate the back with evaluation bacterium colony such as colonial morphology, Grain stain, biochemical reaction and count purpose bacterium bacterium colony number on the flat board, the result represents with Ig cfu/g ight soil.
Statistical analysis
Use SPSS 10.0 statistical softwares, data are represented with x ± s, relatively adopt Student t check between group, and there is statistical significance P＜0.05 for difference.
The gut flora analysis result: dry bacterium powder of the present invention can obviously increase the quantity of interior Bifidobacterium of mouse intestinal and lactobacillus, and enterobacteria and enterococcus are not had obvious influence, shows that described preparation has the gut flora of adjusting function.
Each organizes stool sample gut flora analysis result relatively (x ± s, Ig cfu/g ight soil)
(2) crowd's experiment
Double blind control method is adopted in this test, selects 100 routine experimenters altogether, is divided into test-meal group and control group (each 50 example) at random, edible respectively preparation of the present invention and placebo every day 1 time, each 1 gram.Duration of test does not change original eating habit, normal diet.
The result shows after 14 days, and test-meal group Bifidobacterium and lactobacillus obviously increase, and with there were significant differences before the test-meal (p＜0.05), C.perfringens, bacteroid, enterobacteria, enterococcus do not have significant change.This explanation said composition has the gut flora of adjusting function.
The human body intestinal canal flora testing result (logCFU/g x ± SD)
Embodiment 9: microbial compound preparation of the present invention can reduce blood fat
The microbial compound preparation that utilizes embodiment 1 described fermentation medium to be obtained is studied (experiment in the body, zoopery) to the influence of rat blood serum lipid-metabolism.
With the dead preparation of the thermic of described microbial compound preparation and viable bacteria body preparation is big white mouse with the high cholesterol diet wistar that throws something and feeds simultaneously respectively, and compare with high lipid food group rats gavaged sterilization separated milk and basal feed group rats gavaged sterile saline, carried out of the impact effect analysis of this bacterial strain, shown in experiment grouping situation sees the following form to the rat lipid content.Every group of 10 big white mouse.
Experiment grouping and feeding manner
(bile acid content sees the following form in the influence of AI=(CHO-HDL-C)/HDL-C) and the ight soil to rat blood serum T-CHOL (TC), TG and HDL-C content and atherogenic index during 14d after feeding.
Influence (n=6) to rat blood serum lipid concentration (mmol/L) and atherogenic index AI
To the influence of TBA content in the rat ight soil (mmol/3day, n=6)
The result shows that microbial compound preparation of the present invention has the rising of good inhibition rat blood serum cholesterol concentration and increases the physiologically active of TBA content in the ight soil, the have some improvement activity of lipid-metabolism of tool.
Embodiment 10: microbial compound preparation of the present invention can improve immunity
(1) will utilize microbial compound preparation that embodiment 1 described fermentation medium obtained to mouse peripheral blood CD4+, CD8+ lymphocyte content and ratio Analysis result thereof.
The human body recommended amounts of compound formulation of the present invention is 1g/50Kg every day, and basic, normal, high dosage group dosage is respectively 0.02g/Kg, 0.2g/Kg, 0.6g/Kg, is equivalent to 1,10,30 times of human body recommended amounts respectively.Other establishes the 0.9%NaCl control group.Every group of 10 big white mouse.
Microbial compound preparation of the present invention is gavaged mouse after 10 days continuously, gather each experimental mice peripheral blood sample, on flow cytometer, detect wherein CD4+, CD8+ lymphocyte content after treatment.Detected data are handled with the Cellqust software analysis, and adopt the two-dimensional lattice diagram form to represent.Experimental result such as following table.
To the influence (%) of mouse peripheral blood CD4+, CD8+ lymphocyte and CD19+ lymphocyte content (n=10)
Single lymphocyte with the acquisition after haemolysis is handled of mouse peripheral blood, after specific C D4+ and the dyeing of CD8+ labeling of monoclonal antibody, on flow cytometer, can obtain significantly lymphocyte group of zone according to the big I of cell, after further handling with Cellqust software, the percentage of can obtain hiving off significant CD4+, CD8+ lymphocyte subgroup and each cell subsets.Each the experimental group peripheral blood CD4+ cell percentage that gavages the various dose preparation all is higher than the control group that does not gavage bacterium liquid, wherein has only high dose group CD4+ cell percentage to be significant difference (p＜0.05); And the CD8+ cell percentage of middle dosage group and high dose group is lower than control group, but does not reach the significant difference level as yet.Each dosage group mouse peripheral blood CD4+/CD8+ value also is higher than control group, and wherein middle dosage group and high dose group ratio are significant difference (p＜0.05).In this experiment, give mouse gavaging preparation of the present invention, more all be significantly improved with the control group that does not gavage bacterium liquid to its peripheral blood CD19+ cell subsets percentage.Wherein, middle dosage group and high dose group CD19+ cell subsets percentage are significant difference (p＜0.05), and low dose group is not the significant difference level that reaches.
(2) will utilize the impact analysis of microbial compound preparation that embodiment 1 described fermentation medium obtained to mice serum IgG and intestinal mucosa SIgA content.
Gavage preparation to the once of the present invention in the time of 15 days every day for mouse, the control group serum IgG content all is lower than and does not gavage group, the control group serum IgG content all is lower than and does not gavage group, compare with low dose group, reach significant difference level (p＜0.05), compare with high dose group with middle dosage group, reached utmost point significant difference level (p＜0.01).Gavage to scrape behind the preparation and get intestinal mucosa and measured SIgA content.SIgA content all increases substantially in each dosage group mouse intestinal mucosa, all reaches utmost point significant difference level (p＜0.01), and presents tangible amount-result relation.As seen oral preparation of the present invention can significantly improve the intestinal mucosa immunity function.
Mice serum IgG and intestinal mucosa SIgA assay result
This shows that preparation of the present invention has certain effect to mouse humoral immune and intestinal mucosa local immunity, for the exploitation of further probio and beneficial hair tonic kefir milk is laid a good foundation.
Embodiment 11: microbial compound preparation of the present invention can reduce pork fat content
The microbial compound preparation that utilizes embodiment 1 described fermentation medium to be obtained is studied the influence of weight gain of piglets and fat content.
Select the wean of 35 ages in days for use, the white X Da Bai hybridization of the length of body weight about 9kg piglet totally 30, is divided into 3 groups, 10 every group at random as experimental animal.
Organize in contrast with the basal feed group, other establishes low, high dose group.
Experiment grouping and feeding manner
The weightening finish of experiment pig and fat content
This shows that preparation of the present invention can reduce the fat content of pork, produce and obtain low fat pork.
Embodiment 12: microbial compound preparation of the present invention is to the effect influence of fryer
With the effect influence of the microbial compound preparation that utilizes embodiment 1 described fermentation medium to be obtained to fryer.
Select for use 1 age in days commodity to mix 300 of healthy chickens, be divided into 3 groups at random, 100 every group for the Chinese mugwort denapon.
Organize in contrast with the basal feed group, other establishes low, high dose group.Experiment grouping and feeding manner are with embodiment 12.
Microbial compound preparation is to the influence for the examination meat chicken production performance
By The above results as seen, microbial compound preparation of the present invention can promote body weight gain, reduces death rate of the onset.
Based on above all advantages, microbial compound preparation of the present invention will bring revolutionary change to the market of traditional pharmacy, feed, health products and related industry, and market prospects are wide.
The foregoing description explanation product of the present invention has the better and more function than prior art really.
The application's embodiment only is used for illustrating the present invention, and is not as limitation of the invention, as long as in essential scope of the present invention, all will fall into the scope of protection of present invention to variation, the modification of the above embodiment.
Claims (8)
1. a microbial compound preparation that contains lactobacillus acidophilus is characterized in that, described preparation contains the lactobacillus acidophilus fermentation culture medium, and as active ingredient, the viable count of lactobacillus acidophilus is greater than 7.7 * 10 in the described microbial compound preparation with lactobacillus acidophilus
10Cfu/g, average grain diameter is below the 10 μ m, and described lactobacillus acidophilus fermentation culture medium is to cultivate to obtain in the fermentation medium that contains porous-starch 3%-5%, skimmed milk power 4%-5%, defatted soy flour 5%-7%, tapioca starch 4%-6%, papaya powder 3%-5%, glutamic acid soda 1%, glucose 1%-2%, salt 0.8%.
2. the preparation method of the described microbial compound preparation of claim 1, it is characterized in that, the lactobacillus acidophilus nutrient solution that obtains in the seed culture is inoculated in fermentation medium with 1-5%, continuously fermented 48 hours, with 120 ℃-150 ℃ of inlet temperatures, outlet temperature is that 65 ℃-80 ℃ spray dryer carries out spray-drying.
3. the product that contains the described microbial compound preparation of claim 1.
4. product according to claim 3 is characterized in that, described product can be pharmaceutical preparation, food, health products and feed etc.
5. the application of the described microbial compound preparation of claim 1.
6. application according to claim 5 is characterized in that, to be microbial compound preparation contain application in the product of described preparation in preparation in described application.
7. the purposes of the described microbial compound preparation of claim 1 is characterized in that, described compound formulation can be used for preparing and improves immunity, reduces blood fat and improve medicine and/or food and/or the health products and/or the nutritious supplementary pharmaceutical of gut flora.
8. the purposes of the described microbial compound preparation of claim 1 is characterized in that, described compound formulation can be used as feed addictive
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| CN104432001A (en) * | 2013-09-18 | 2015-03-25 | 深圳华大基因科技有限公司 | Edible composition and use thereof |
| CN104432001B (en) * | 2013-09-18 | 2018-01-12 | 深圳华大基因科技有限公司 | Edible composition and application thereof |
| CN105770355A (en) * | 2016-03-17 | 2016-07-20 | 青岛根源生物技术集团有限公司 | Compound preparation for preventing necrotic enteritis of broilers and application of compound preparation |
| CN108018235A (en) * | 2017-12-18 | 2018-05-11 | 江南大学 | A kind of high activity lactobacillus bacterium powder spray drying production technology and application |
| CN108070542A (en) * | 2017-12-18 | 2018-05-25 | 江南大学 | A kind of high-activity bifidobacterium powder spray drying production technology and application |
| CN111528478A (en) * | 2020-05-13 | 2020-08-14 | 深圳市东荣生物科技有限责任公司 | Compound beneficial microbial preparation for treating constipation and preparation method thereof |
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