CN102210334A - New application of euphorbia helioscopia in plant mite killing - Google Patents
New application of euphorbia helioscopia in plant mite killing Download PDFInfo
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- CN102210334A CN102210334A CN2011101003499A CN201110100349A CN102210334A CN 102210334 A CN102210334 A CN 102210334A CN 2011101003499 A CN2011101003499 A CN 2011101003499A CN 201110100349 A CN201110100349 A CN 201110100349A CN 102210334 A CN102210334 A CN 102210334A
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Abstract
The invention discloses application of euphorbia helioscopia in preparation of a curative plant mite killing agent, in particular a tetranychus cinnabarinus killing agent. A method for preparing the euphorbia helioscopia into the mite killing agent comprises the following steps of: drying the euphorbia helioscopia plant, and crushing the euphorbia helioscopia until the granularity reaches 80 meshes; weighing the crushed euphorbia helioscopia, putting the euphorbia helioscopia into a stirring container, adding an organic solvent in an amount which is 6 times weight of the euphorbia helioscopia, and extracting the euphorbia helioscopia with stirring for 3 times and 24 hours every time in a thermostatic water bath of 60 DEG C; performing separation and filtration to obtain extract, evaporating and concentrating the extract under the vacuum degree of 0.07MPa, and reclaiming the organic solvent in the extract to obtain the concentrated solution of the euphorbia helioscopia extract; and concentrating the concentrated solution under reduced pressure to obtain 75.9 percent of solid, and adding a solubilizing agent in an amount which is 0.2 times weight of the concentrated solution and an emulsifying agent in an amount which is 0.2 times weight of the concentrated solution to dissolve the solid to prepare euphorbia helioscopia plant mite killing spray. The application has the advantages that the environment is not polluted, the pesticide resistance is not easily produced, the mite killing spray has remarkable mite killing effect, the extraction is simple and feasible, and the preparation method is favorable for industrialized production.
Description
Technical field
The present invention relates to a kind of new purposes of wartwort, particularly a kind of wartwort is used for the new purposes of plant acaricidal.
Background technology
Phytophagous mites have individual little, breeding is fast, development duration is short, sphere of action is little, adaptability is strong, mutation rate is high and characteristics such as pesticide resistance easily take place, and is the pest group of the most difficult control of generally acknowledging.
The agricultural miticide of initial stage use mainly is the inorganic compound and Dormant oils (mainly the being machine oil) emulsion of Cosan and polysulfide thereof and so in the world.Enter the 1950's, efficient organic miticide occurs one after another, come out until the chrysanthemum ester class miticide of super-high-efficient, has driven miticide has moved towards efficient organic compound from the inorganic compound of poor efficiency ranks.But this type of acaricidal frequent use has caused environmental pollution, and existent environment of people has been proposed challenge.Next is because the growth time of a generation of mite class is shorter, can produce resistance rapidly to original miticide.Because the development of drug resistance of evil mite is very fast, just requires to accelerate development novel anti-mite agent.Screening pesticide activity component hit rate more and more low today in synthetic compound, people pay attention to seeking the research approach of new drug more from natural products.From natural plants, seek to kill acaricidal composition, the research of carrying out botanical pesticide be the most tempting also be the most popular topic.Compare with synthetic organic pesticide, botanical pesticide has characteristics such as the high and low poison of selectivity, easily degraded, the difficult generation of pest resistance.The main component of botanical pesticide is naturally occurring compound, these active substances mainly are made up of elements such as C, H, O, derive from nature, in the evolution of long period of time process, formed its fixing energy and material cyclic metabolic pathway, so when using, it is residual to be difficult for generation, can not cause the biological concentration phenomenon.
The exploitation botanical pesticide mainly is the secondary metabolites that utilizes in the plant corpus.Secondary substance surpasses 400,000 kinds in the plant, and wherein there are 10,000 kinds approximately and identified molecular structure, mainly be alkaloid, terpene and phenols.Along with the development of high flux screening technology, increasing zoophobous secondary substance is found, and these make people recognize the great potential of appliable plant secondary substance Pest Control.Therefore, the research of both at home and abroad vegetable source pesticide being killed the mite material in recent years grows to even greater heights.
Summary of the invention
The object of the invention is to provide a kind of wartwort to be used for the new purposes of plant acaricidal.
Plant acaricidal of the present invention can be that Tetranychus cinnabarinus becomes mite.
Described wartwort is added conventional auxiliary material, according to common process, make various miticides, as spray, missible oil, suspending agent or pulvis.
The preparation method that the present invention makes plant acaricide with wartwort is: with the oven dry of 100-300 weight portion wartwort plant, be crushed to 40～120 orders; Take by weighing wartwort after the pulverizing in stirred vessel, add the organic solvent of 4～8 times of weight, stirring and leaching is 2-4 time in 40～80 ℃ of thermostat water baths, each 12～36h; Separating and filtering gets leaching liquor, merges leaching liquor, and leaching liquor is evaporation and concentration under vacuum 0.05Mpa～0.09Mpa, reclaims the organic solvent in the leaching liquor, obtains the concentrate of Herba Euphorbiae Helioscopiae extract; Concentrate is evaporated to solid content 70-85%, adds the solubilizer of solid content 0.1-0.3 times of weight and the emulsifiers dissolve of adding solid content 0.1-0.3 times of weight, according to common process, adds conventional auxiliary material and makes spray, missible oil, suspending agent or pulvis.
The present invention makes spray preparing process with wartwort and is preferably: with the oven dry of 200 weight portion wartwort plants, be crushed to 80 orders; Take by weighing wartwort after the pulverizing in stirred vessel, add the organic solvent of 6 times of weight, stirring and leaching is 3 times in 60 ℃ of thermostat water baths, each 24h; Separating and filtering gets leaching liquor, and leaching liquor is evaporation and concentration under vacuum 0.07Mpa, reclaims the organic solvent in the leaching liquor, obtains the concentrate of Herba Euphorbiae Helioscopiae extract; Concentrate is evaporated to solid content 75.9%, adds the solubilizer of solid content 0.2 times of weight and the emulsifiers dissolve of adding solid content 0.2 times of weight, according to common process, adds conventional auxiliary material and makes spray, missible oil, suspending agent or pulvis.
Solvent of the present invention is methyl alcohol or chloroform; Described solubilizer is neopelex or Potassium dodecylbenzenesulfonate; Described emulsifier is an odium stearate.
The invention provides a kind ofly, the comprehensive regulation of ecotope long term growth, human beings'health and disease is all had profound significance person poultry safety, environmentally friendly harmonious agricultural chemicals.Compare with traditional agricultural chemicals, the present invention has following characteristics and advantage: (1) wartwort is the plant of occurring in nature, so its extract is easy to decompose under field conditions (factors), to environment and ecosystem safety on every side, does not have problem of environmental pollution; (2) the wartwort plant acaricide is a kind of multicomponent mixture, and the mite class is had a plurality of action sites, therefore is difficult for developing immunity to drugs; (3) experiment showed, that the wartwort plant acaricide has significant miticidal effect, has the chemical pesticide characteristics of high efficiency; (4) extraction of wartwort effective components in plants is simple, helps carrying out suitability for industrialized production.
Following experimental example and embodiment are used to further specify the present invention, but are not limited to the present invention.
Experimental example:
1, experimental technique
(1) preparation method of wartwort (according to the preparation of embodiment 1 method)
Take by weighing wartwort 200g, oven dry is crushed to 80 orders; Take by weighing wartwort after the pulverizing in stirred vessel, add the methyl alcohol of 6 times of weight, stirring and leaching is 3 times in 60 ℃ of thermostat water baths, each 24h; Separating and filtering gets leaching liquor, and leaching liquor is evaporation and concentration under vacuum 0.07Mpa, reclaims the methanol solvate in the leaching liquor, obtains the concentrate of Herba Euphorbiae Helioscopiae extract; Concentrate is evaporated to solid content 75.9%, adds the neopelex of solid content 0.2 times of weight and the odium stearate dissolving of adding solid content 0.2 times of weight, makes wartwort plant acaricidal spray.
(2) indoor bioactive mensuration
Slide infusion process a: end that active, the of the same size female one-tenth mite of health back is sticked at slide downwards with two-sided tape paper, and under anatomical lens, reject the killed or wounded individuality, guarantee that every slide tetranychid number is 30, take out after in soup, soaking 5s, sop up floating medicine gently with filter paper, observe its death condition behind 24h, the moving person of contact is for living, motionless person is dead, the calculation correction lethality.
2, experimental result
This miticide becomes the biologically active of mite to Tetranychus cinnabarinus: 24h corrected mortality 73.27%.In order further to investigate this acaricidal virulence, set the concentration gradient of 1mg/mL, 2mg/mL, 4mg/mL, 6mg/mL, 8mg/mL, adopt the slide infusion process to measure to such an extent that virulence regression equation is y=1.1717x+5.6366, lethal concentration of 50 LC50 is 0.2862mg/mL, correlation coefficient r=0.9789,95% confidence limit is 0.0519～1.5792, and as seen, this is acaricidal higher biologically active.
Following embodiment all can test the effect of above-mentioned experimental example.
Embodiment 1: spray
Take by weighing wartwort 200g, oven dry is crushed to 80 orders; Take by weighing wartwort after the pulverizing in stirred vessel, add the methyl alcohol of 6 times of weight, stirring and leaching is 3 times in 60 ℃ of thermostat water baths, each 24h; Separating and filtering gets leaching liquor, leaching liquor is evaporation and concentration under vacuum 0.07Mpa, reclaim the methanol solvate in the leaching liquor, obtain the concentrate of Herba Euphorbiae Helioscopiae extract, concentrate is evaporated to solid content 75.9%, add the neopelex of solid content 0.2 times of weight and the odium stearate dissolving of adding solid content 0.2 times of weight, make wartwort plant acaricidal spray.
Embodiment 2: missible oil
Get wartwort 100g, oven dry is crushed to 100 orders; Take by weighing wartwort after the pulverizing in stirred vessel, add the chloroform of 7 times of weight, stirring and leaching is 4 times in 75 ℃ of thermostat water baths, each 15h; Separating and filtering gets leaching liquor, merges leaching liquor, and leaching liquor is evaporation and concentration under vacuum 0.08Mpa, reclaims the chloroform solvent in the leaching liquor, obtains the concentrate of Herba Euphorbiae Helioscopiae extract; Concentrate is evaporated to solid content 78.6%, adds the Potassium dodecylbenzenesulfonate of solid content 0.25 times of weight and the odium stearate dissolving of adding solid content 0.15 times of weight, according to common process, adds conventional auxiliary material and makes missible oil.
Embodiment 3: suspending agent
Get wartwort 150g, oven dry is crushed to 60 orders; Take by weighing wartwort after the pulverizing in stirred vessel, add the methyl alcohol of 5 times of weight, stirring and leaching is 2 times in 45 ℃ of thermostat water baths, each 30h; Separating and filtering gets leaching liquor, merges leaching liquor, and leaching liquor is evaporation and concentration under vacuum 0.06Mpa, reclaims the methanol solvate in the leaching liquor, obtains the concentrate of Herba Euphorbiae Helioscopiae extract; Concentrate is evaporated to solid content 82.5%, adds the neopelex of solid content 0.15 times of weight and the odium stearate dissolving of adding solid content 0.25 times of weight, according to common process, adds conventional auxiliary material and makes suspending agent.
Embodiment 4 pulvis
Get wartwort 250g, add conventional auxiliary material according to common process and make pulvis.
Claims (10)
1. the application of wartwort in preparation treatment plant acaricidal.
2. application as claimed in claim 1 is characterized in that described mite extremely is meant that Tetranychus cinnabarinus becomes mite.
3. application as claimed in claim 1 or 2 is characterized in that wartwort is added conventional auxiliary material, according to common process, makes spray, missible oil, suspending agent or pulvis.
4. application as claimed in claim 1 or 2 is characterized in that wartwort is made acaricidal preparation method is: with the oven dry of 100-300 weight portion wartwort plant, be crushed to 40～120 orders; Take by weighing wartwort after the pulverizing in stirred vessel, add the organic solvent of 4～8 times of weight, stirring and leaching is 2-4 time in 40～80 ℃ of thermostat water baths, each 12～36h; Separating and filtering gets leaching liquor, merges leaching liquor, and leaching liquor is evaporation and concentration under vacuum 0.05Mpa～0.09Mpa, reclaims the organic solvent in the leaching liquor, obtains the concentrate of Herba Euphorbiae Helioscopiae extract; Concentrate is evaporated to solid content 70-85%, adds the solubilizer of solid content 0.1-0.3 times of weight and the emulsifiers dissolve of adding solid content 0.1-0.3 times of weight, according to common process, adds conventional auxiliary material and makes spray, missible oil, suspending agent or pulvis.
5. application as claimed in claim 4 is characterized in that wartwort is made acaricidal preparation method is: with the oven dry of 200 weight portion wartwort plants, be crushed to 80 orders; Take by weighing wartwort after the pulverizing in stirred vessel, add the organic solvent of 6 times of weight, stirring and leaching is 3 times in 60 ℃ of thermostat water baths, each 24h; Separating and filtering gets leaching liquor, and leaching liquor is evaporation and concentration under vacuum 0.07Mpa, reclaims the organic solvent in the leaching liquor, obtains the concentrate of Herba Euphorbiae Helioscopiae extract; Concentrate is evaporated to solid content 75.9%, adds the solubilizer of solid content 0.2 times of weight and the emulsifiers dissolve of adding solid content 0.2 times of weight, according to common process, adds conventional auxiliary material and makes spray, missible oil, suspending agent or pulvis.
6. application as claimed in claim 4 is characterized in that the organic solvent among the described preparation method is methyl alcohol or chloroform; Described solubilizer is neopelex or Potassium dodecylbenzenesulfonate; Described emulsifier is an odium stearate.
7. application as claimed in claim 5 is characterized in that the organic solvent among the described preparation method is methyl alcohol or chloroform; Described solubilizer is neopelex or Potassium dodecylbenzenesulfonate; Described emulsifier is an odium stearate.
8. a plant acaricide is characterized in that, this miticide is made by following method: with the oven dry of 100-300 weight portion wartwort plant, be crushed to 40～120 orders; Take by weighing wartwort after the pulverizing in stirred vessel, add the organic solvent of 4～8 times of weight, stirring and leaching is 2-4 time in 40～80 ℃ of thermostat water baths, each 12～36h; Separating and filtering gets leaching liquor, merges leaching liquor, and leaching liquor is evaporation and concentration under vacuum 0.05Mpa～0.09Mpa, reclaims the organic solvent in the leaching liquor, obtains the concentrate of Herba Euphorbiae Helioscopiae extract; Concentrate is evaporated to solid content 70-85%, adds the solubilizer of solid content 0.1-0.3 times of weight and the emulsifiers dissolve of adding solid content 0.1-0.3 times of weight, according to common process, adds conventional auxiliary material and makes spray, missible oil, suspending agent or pulvis.
9. plant acaricide as claimed in claim 8 is characterized in that, this miticide is made by following method: with the oven dry of 200 weight portion wartwort plants, be crushed to 80 orders; Take by weighing wartwort after the pulverizing in stirred vessel, add the organic solvent of 6 times of weight, stirring and leaching is 3 times in 60 ℃ of thermostat water baths, each 24h; Separating and filtering gets leaching liquor, and leaching liquor is evaporation and concentration under vacuum 0.07Mpa, reclaims the organic solvent in the leaching liquor, obtains the concentrate of Herba Euphorbiae Helioscopiae extract; Concentrate is evaporated to solid content 75.9%, adds the solubilizer of solid content 0.2 times of weight and the emulsifiers dissolve of adding solid content 0.2 times of weight, according to common process, adds conventional auxiliary material and makes spray, missible oil, suspending agent or pulvis.
10. plant acaricide as claimed in claim 8 or 9 is characterized in that described organic solvent is methyl alcohol or chloroform; Described solubilizer is neopelex or Potassium dodecylbenzenesulfonate; Described emulsifier is an odium stearate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101003499A CN102210334A (en) | 2011-04-21 | 2011-04-21 | New application of euphorbia helioscopia in plant mite killing |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101003499A CN102210334A (en) | 2011-04-21 | 2011-04-21 | New application of euphorbia helioscopia in plant mite killing |
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| CN102210334A true CN102210334A (en) | 2011-10-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2011101003499A Pending CN102210334A (en) | 2011-04-21 | 2011-04-21 | New application of euphorbia helioscopia in plant mite killing |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103081953A (en) * | 2013-02-18 | 2013-05-08 | 青岛农业大学 | Liquor preparation of water extract of wartwort as well as preparation method thereof |
| CN103081954A (en) * | 2013-02-18 | 2013-05-08 | 青岛农业大学 | Alcoholic solution preparation of wartwort secondary metabolism product and preparation method thereof |
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| CN1194782A (en) * | 1997-04-01 | 1998-10-07 | 云南省烟草科学研究所 | Tobacco plant protection insecticide and production method thereof |
| CN101647472A (en) * | 2007-07-31 | 2010-02-17 | 北京农学院 | Mentha arvensisi vegetable acaricide and preparation method thereof |
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2011
- 2011-04-21 CN CN2011101003499A patent/CN102210334A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1194782A (en) * | 1997-04-01 | 1998-10-07 | 云南省烟草科学研究所 | Tobacco plant protection insecticide and production method thereof |
| CN101647472A (en) * | 2007-07-31 | 2010-02-17 | 北京农学院 | Mentha arvensisi vegetable acaricide and preparation method thereof |
Non-Patent Citations (2)
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| 兰艳: "自制家用花卉农药", 《农村新技术》 * |
| 赵莉蔺等: "植物源杀螨剂的研究进展", 《植物医生》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103081953A (en) * | 2013-02-18 | 2013-05-08 | 青岛农业大学 | Liquor preparation of water extract of wartwort as well as preparation method thereof |
| CN103081954A (en) * | 2013-02-18 | 2013-05-08 | 青岛农业大学 | Alcoholic solution preparation of wartwort secondary metabolism product and preparation method thereof |
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Application publication date: 20111012 |