CN102209780B - 经改进的核黄素生产 - Google Patents

经改进的核黄素生产 Download PDF

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Publication number
CN102209780B
CN102209780B CN200980144726.XA CN200980144726A CN102209780B CN 102209780 B CN102209780 B CN 102209780B CN 200980144726 A CN200980144726 A CN 200980144726A CN 102209780 B CN102209780 B CN 102209780B
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riboflavin
rib
leader
modified
rib leader
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CN102209780A (zh
Inventor
马丁·莱玛恩
迈克尔·汉斯
汉斯-彼得·霍曼
迪特马尔·卢达尔特
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DSM IP Assets BV
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DSM IP Assets BV
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P25/00—Preparation of compounds containing alloxazine or isoalloxazine nucleus, e.g. riboflavin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN200980144726.XA 2008-11-07 2009-11-09 经改进的核黄素生产 Active CN102209780B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08019492A EP2186880A1 (en) 2008-11-07 2008-11-07 Improved production of riboflavin
EP08019492.1 2008-11-07
PCT/EP2009/064825 WO2010052319A1 (en) 2008-11-07 2009-11-09 Improved production of riboflavin

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201310713983.9A Division CN103757000A (zh) 2008-11-07 2009-11-09 经改进的核黄素生产

Publications (2)

Publication Number Publication Date
CN102209780A CN102209780A (zh) 2011-10-05
CN102209780B true CN102209780B (zh) 2016-02-10

Family

ID=40329198

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201310713983.9A Pending CN103757000A (zh) 2008-11-07 2009-11-09 经改进的核黄素生产
CN200980144726.XA Active CN102209780B (zh) 2008-11-07 2009-11-09 经改进的核黄素生产

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CN201310713983.9A Pending CN103757000A (zh) 2008-11-07 2009-11-09 经改进的核黄素生产

Country Status (7)

Country Link
US (1) US10633684B2 (OSRAM)
EP (2) EP2186880A1 (OSRAM)
JP (1) JP5787089B2 (OSRAM)
KR (1) KR101726886B1 (OSRAM)
CN (2) CN103757000A (OSRAM)
ES (1) ES2648045T3 (OSRAM)
WO (1) WO2010052319A1 (OSRAM)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101335853B1 (ko) 2011-12-01 2013-12-02 씨제이제일제당 (주) Ｌ-아미노산 및 리보플라빈을 동시에 생산하는 미생물 및 이를 이용한 ｌ-아미노산 및 리보플라빈을 생산하는 방법
WO2015118126A1 (en) * 2014-02-07 2015-08-13 Dsm Ip Assets B.V. Improved bacillus host
CN103865944B (zh) * 2014-02-20 2016-01-20 天津大学 一种生产核黄素的大肠杆菌菌株及构建方法及用途
KR102561864B1 (ko) * 2014-11-19 2023-08-01 바스프 에스이 Rib７ 프로모터를 사용하여 유전자 발현을 하향조절하기 위한 에레모테시움의 유전적 변형
WO2017036903A1 (en) * 2015-08-28 2017-03-09 Dsm Ip Assets B.V. Improved vitamin production
KR101844778B1 (ko) * 2017-01-18 2018-04-04 주식회사한국야쿠르트 비타민 b2 생합성능이 매우 우수한 락토바실러스 플란타룸 hy7715 및 이를 유효성분으로 함유하는 제품
CN108330136A (zh) * 2017-05-05 2018-07-27 北京航空航天大学 一种构建产黄素单核苷酸的工程菌株的方法及其应用
CN111094576A (zh) * 2017-09-13 2020-05-01 丹尼斯科美国公司 用于增加芽胞杆菌属中蛋白质产生的经修饰的5′-非翻译区(utr)序列
MX2020010422A (es) 2018-04-05 2020-10-28 Dsm Ip Assets Bv Alfa-amilasa maltogenica variante.
EP3880835A1 (en) * 2018-11-15 2021-09-22 DSM IP Assets B.V. Improved production of riboflavin
BR112022026306A2 (pt) 2020-06-23 2023-01-17 Dsm Ip Assets Bv Processo de fermentação
CN114592000B (zh) * 2020-12-03 2023-07-21 上海市农业科学院 一种六基因组合在水稻种子中提高vb2含量的应用及方法
CN112538453B (zh) * 2020-12-15 2022-07-29 通辽梅花生物科技有限公司 一种产核黄素的枯草芽孢杆菌及其构建方法与应用
CN116463305B (zh) * 2023-06-15 2023-10-17 北京易醒生物科技有限公司 一种提高用于乙醇氧化的醇氧化酶表达量的方法、优化的核黄素生物合成基因
CN117660577B (zh) * 2023-12-08 2024-06-25 苏州华赛生物工程技术有限公司 LtaSA蛋白或其编码基因在核黄素生产中的应用
CN117568433B (zh) * 2024-01-15 2024-04-16 黑龙江新和成生物科技有限公司 一种提高枯草芽孢杆菌发酵生产核黄素的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1049185A (zh) * 1989-06-22 1991-02-13 霍夫曼-拉罗奇有限公司 高产核黄素的细菌菌株
US20030232406A1 (en) * 1989-06-22 2003-12-18 Roche Vitamins, Inc. Bacterial strains which overproduce riboflavin

Family Cites Families (6)

* Cited by examiner, † Cited by third party
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US6582908B2 (en) * 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5474796A (en) * 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US6656721B1 (en) 2000-08-08 2003-12-02 Roche Vitamins, Inc. Polynucleotide portions of the biotin operon from B. subtilis for use in enhanced fermentation
WO2004106557A2 (en) 2003-06-02 2004-12-09 Dsm Ip Assets B.V. Thiamin production by fermentation
JP4713469B2 (ja) 2003-06-18 2011-06-29 ディーエスエム アイピー アセッツ ビー．ブイ． 胞子形成できない微生物を用いたパントテネート（Ｐａｎｔｏｔｈｅｎａｔｅ）の製造
US8257944B2 (en) 2005-11-02 2012-09-04 Dsm Ip Assets B.V. Modified transketolase and use thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1049185A (zh) * 1989-06-22 1991-02-13 霍夫曼-拉罗奇有限公司 高产核黄素的细菌菌株
US20030232406A1 (en) * 1989-06-22 2003-12-18 Roche Vitamins, Inc. Bacterial strains which overproduce riboflavin

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Relationship between the secondary structure and the regulatory activity of the leader region of the riboflavin biosynthesis operon in Bacillus subtilis;Mironov, AS;《RUSSIAN JOURNAL OF GENETICS》;20080430;第44卷(第4期);399-404 *
强启动子枯草芽孢杆菌核黄素操纵子整合型载体的构建并在枯草芽孢杆菌中的表达;周世奇;《中国优秀硕士学位论文全文数据库》;20070515;全文 *

Also Published As

Publication number Publication date
KR101726886B1 (ko) 2017-04-13
KR20110093847A (ko) 2011-08-18
CN103757000A (zh) 2014-04-30
US10633684B2 (en) 2020-04-28
US20110312025A1 (en) 2011-12-22
EP2356211B1 (en) 2017-09-20
JP5787089B2 (ja) 2015-09-30
EP2186880A1 (en) 2010-05-19
EP2356211A1 (en) 2011-08-17
ES2648045T3 (es) 2017-12-28
WO2010052319A1 (en) 2010-05-14
CN102209780A (zh) 2011-10-05
JP2012507984A (ja) 2012-04-05

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