CN102199520A - Full-automatic microbial fermentation case for farm places and its application method - Google Patents

Full-automatic microbial fermentation case for farm places and its application method Download PDF

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Publication number
CN102199520A
CN102199520A CN2010101291006A CN201010129100A CN102199520A CN 102199520 A CN102199520 A CN 102199520A CN 2010101291006 A CN2010101291006 A CN 2010101291006A CN 201010129100 A CN201010129100 A CN 201010129100A CN 102199520 A CN102199520 A CN 102199520A
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fermenting case
temperature
fermentation
heating
novel microorganism
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CN2010101291006A
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段云峰
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14Incubators; Climatic chambers

Abstract

A novel full-automatic bidirectional hot air heating microbial fermentation case for farm places comprises a casing, a power supply controller, a digit display thermostat, a temperature control probe, a digit display time controller, an alarm device, separator plates, fans, electrothermal tubes, UV bactericidal lamps, fermentation racks and spliter plates. By employing bidirectional hot air heating in both transverse and vertical directions, the heating temperature is rapidly elevated, hot air cycle efficiency is high and the temperature is uniform. By the combination of the pasteurization method and UV disinfection, disinfection and fermentation processes can be automatically completed. Functional components such as at least two UV disinfection lamps, at least two electrothermal tubes in transverse and vertical directions and at least two fans can ensure the machine to operate for a long time. The microbial fermentation case provided by the invention has advantages of simple integral structure, low manufacture cost, convenient operation, stable and accurate temperature controlling, wide usage range, few microbe production processes, low energy consumption, low cost, large quantity of live bacterium and multiple producible live bacterium, and is very suitable for farm places in the south and north regions to ferment and produce high-activity microbes all the year round.

Description

Plant full automatic microorganism fermenting case and using method thereof
Technical field
Patent of the present invention relates to a kind of microbial fermentation equipment and using method thereof, and specifically a kind of plant that is applicable to of two-way hot blast heating that adopts produces the new automatic microbial fermentation case of active microbiological preparations and the method that this fermenting case of use is produced active microbiological preparations.
Background technology
Living microorganism is more common in the market microbial preparation, and the common field of living microorganism comprises sewage disposal, food-processing, fermented feed, livestock and poultry cultivation, aquaculture, biological pesticide, biological organic fertilizer etc.Wherein, the probiotic bacterium that is applied to food and feedstuff industry is exactly a kind of by improving, balance host's intestinal flora, thus the host is played the living microorganism of beneficial effect.
Along with people are heightened to the demand of animal products, large-scale cultural technique has occupied main flow gradually.Carry out highdensity raising in the narrow space, air dirt in causing giving up, movement overflows, the temperature height, the humidity height causes animal easily sick, and the generation of pestilence is also more and more general.For a long time, people attempt in feed adding microbiotic or some chemical additives attempt to resist the pathogeny microorganism that influence growth of animals or poultry even cause livestock death, prevent pig, chicken sick.Antibiotic usage quantity and kind are more and more, and the resistance problem is more and more serious, and antibiotic remains is also more and more.In addition, along with increasing of feed additive matter, these materials can't be estimated the potential hazard that the mankind's healthy and ecotope causes.
19th-century sixties, once widelyd popularize fermented feed in China, the end of the seventies, the living microorganism cultural technique had to a certain degree influence to Chinese aquaculture so far.The common fermentation feed is a kind of natural fermented at random fully, be subjected to the variable effect of temperature, humidity, pH value easily, great variety also can take place in the microbe population and the kind of fermentation, the feed mouthfeel of producing, smell or color distortion are very big, and fermenting process also may produce toxic substance harm animal health.And the condition of organism of fermentation that fermentative feedstuff of microbe is used and fermentation all is controlled, therefore can produce steady quality, safe and reliable fermented feed.
The core theory of fermentative feedstuff of microbe cultural technique foundation is the intravital microecological balance of animal.The microecological balance of animal digestive tract has determined the healthy state of animal.Under the normal circumstances, the bacterium of enteron aisle (harmful bacterium, probiotics and neutral bacterium) keeps certain balance, keeps the function that normal enteron aisle is digested and assimilated.Owing to when a variety of causes causes the normal microflora of enteron aisle to change, just produce little disruption of ecological balance, and then cause various diseases.By the fermentative feedstuff of microbe of feeding, the probiotics in the feed can be regulated the bacterium colony balance of animal digestive tract, safeguards the normal micro-ecological environment of animal, improves immunizing power, thereby resists the generation of various diseases.Fermentative feedstuff of microbe has many-sided advantage: can produce low microbiotic even the residual animal products of antibiotic-free, ensure food safety, simultaneously ecotope is not damaged again, had increasing plant to begin to select fermentative feedstuff of microbe.
The range of application of living microorganism is very extensive, is most commonly used to fermented feed in plant, the prevention livestock and poultry, and water body health is kept in aquaculture, makes microbial pesticide, produces microbial organic fertilizer, produces aspects such as biogas.Living microorganism is the indispensable production means of modern agriculture.
Though fermentative feedstuff of microbe has many benefits, still there are some limiting factors to hamper the development of fermentative feedstuff of microbe.At present, there are three shortcomings in the production of fermentative feedstuff of microbe: the one, and the bacterial classification that the production fermented feed is used requires height to fermentation installation, invests too big.Carry out microbial fermentation and need comparatively strict aseptic condition, need special microbial fermentation equipment, sterilization and packaging facilities or the like, also to build high-level factory building, be equipped with the microbial fermentation operator through strict training, the very high bacterial classification price that causes fermented feed to be used of cost of investment is higher.The 2nd, produce the strain fermentation device structure complexity that fermented feed is used, the cost height.Present fermentation equipment mainly is a microorganism fermentation tank, generally includes water and air supply system, sterilising system, temperature controlling system, stirring system and delivery system etc., complex structure, Production Flow Chart complexity.In addition, production process needs High Temperature High Pressure and gnotobasis, so energy consumption cost is very high.General plant is difficult to possess working condition.The 3rd, the bacterial activity that is used for fermentative feedstuff of microbe is not high.Because the condition of microorganism existence is comparatively harsh, the improper forfeiture that all can cause microorganism active of temperature humidity.The probiotics of using mostly is pulvis now, and number of viable is limited, so result of use is had a greatly reduced quality.In addition, it is the process of a high energy consumption that active bacteria formulation is dried to the pulvis ability that is easy to transport and preserve by liquid state, adds transportation cost, so cost promotes significantly, and economic benefit is not high.
In fermentation process, one of of paramount importance technology is sterilization.The most frequently used sterilising method is the heat sterilization method in the microbial fermentation production, with boiling water, steam and steam pressurization sterilization.Steam pressurization sterilising effect is best, and available free flowing steam sterilization also can (generally use 1 kg/cm in the high compressed steam pot 2) sterilization, its vapour temperature can reach 121 ℃, heat-stable gemma all can be killed in 30 minutes.But to some easily by high pressure destructive material, as some sugar or nitrogen-containing organic compound, should be in 0.6 kg/cm 2(110 ℃) sterilization is 15~30 minutes under the pressure.The most frequently used in plant produced is free flowing steam sterilization, utilizes direct-fired circulation water vapour killing microorganisms.Pasteurization is dairy products enterprise a kind of sterilising method commonly used, comprise dual mode: 1. low temperature long time treatment: handled 30 minutes down at 61.7~65 ℃, adopt this method to handle milk, can kill various growth form pathogenic bacterium in the milk, sterilizing efficiency can reach 97.3%~99.9%, residual just part thermophile bacteria and thermotolerance bacterium and gemma etc. after sterilizing, but these bacterium majorities are milk-acid bacterias, and milk-acid bacteria is not only harmless good for health on the contrary to the people; 2. handle between high temperature, short time: at 71.6 ℃ or slightly handled 15 minutes under the high-temperature.Its sterilizing time is shorter, and working efficiency is higher.This method is usually used in the sterilization of dairy products, can preserve more nutritive substance.Because pasteurization only adopts lower temperature control just can finish sterilization process, and can preserve more nutritive substance, therefore, the equipment that adopts pasteurization to need is simple, can control automatically, production technique is grasped easily, and energy consumption is low, less investment is highly suitable in plant's microbial fermentation production material being sterilized.
The most frequently used ultra-violet sterilization is exactly by ultraviolet irradiation in the radiation sterilization, destroys and change DNA (thymus nucleic acid) structure of microorganism, makes the dead at once or irreproducible offspring of bacterium, reaches germ-resistant purpose.What really have germicidal action is that (wavelength 100~275nm is called shortwave sterilization ultraviolet ray again in the UVC ultraviolet ray.), absorbed by the DNA of organism because the C-band ultraviolet ray is very easy, best with the ultraviolet ray about 253.7nm especially.Ultra-violet sterilization belongs to pure physical disinfection method, has simple and convenient, broad-spectrum high efficacy, non-secondary pollution, is convenient to management and realizes advantages such as automatization.
In addition, adopt the fermenting case of hot blast heating relatively simple for structure in the market, defective the such as therefore heat that the electrothermal tubes that adopt the bottom the produce more natural convection heat supply that makes progress exists fermenting case internal temperature difference big, and temperature distributing disproportionation is even.Hindered the natural convection of hot blast when the more material of filling, therefore the situation of more having aggravated temperature distributing disproportionation can not satisfy in the real attenuation production the accurately requirement of control of temperature.
Summary of the invention
In order to overcome existing microbial fermentation bacterial classification price height, number of viable is few, the production unit complex structure, Production Flow Chart is many, and the energy consumption height is invested big deficiency, in order to adapt to the actual production environment of plant, it is uneven to improve existing fermenting case heating simultaneously, and temperature is controlled coarse shortcoming.This patent provide a kind of adopt the heating of two-way hot blast new automatic microbial fermentation case and using method thereof, be applicable to that plant produces the high reactivity probiotics.
The technical scheme that this patent technical solution problem is adopted: the structure that this patent adopts comprises described novel microorganism fermenting case, relates to casing, power-supply controller of electric, digital thermostat, temperature control probe, digital time controller, warning device, dividing plate, fan, electrothermal tube, ultraviolet germicidal, fermentation frame and the division plate composition of showing of showing.Described casing can be the profile of vertical on the market or horizontal showcase, for example rectangular build.The said casing of this patent is divided into three layers at least, is preferably three layers, and the upper strata is provided with the needed power-supply controller of electric of fermenting case, numeral demonstration thermostat, numeral demonstration time controller and warning device for controlling layer.The power switch of power controller controls fermenting case; Numeral shows that thermostat shows the actual temperature of fermenting case and temperature is set; Numeral shows that time controller shows the time of fermentation time and setting; Warning device is used to point out temperature to surpass limiting temperature, timing end or fermentation ends.The middle layer is a proving room, is provided with the temperature control probe in the described proving room, and described temperature control probe shows that with the numeral of controlling layer thermostat is connected.Described proving room following and inboard be zone of heating, be provided with dividing plate between proving room and the zone of heating.Respectively be provided with at least two ventilation holes on the dividing plate, be provided with fan in the ventilation hole.Be provided with electrothermal tube in the zone of heating, be used to add warm air, heat is provided.Consider the work-ing life of component,, therefore consider to add corresponding standby component, ensure the machine long-play in order not influence the works better of fermenting case.Other structures all are provided with according to the prior art processes standard.
The using method of fermenting case of the present invention: after connecting power supply, configure the needed temperature and time of microbial fermentation, said electrothermal tube begins to heat up, temperature in the zone of heating begins to rise, from horizontal and vertical introducing proving room, do not stop to circulate guarantees temperature-stable, evenly in the proving room to fan on the dividing plate with the hot blast of zone of heating.Set when requiring when the temperature in the proving room reaches, numeral shows that thermostat cuts off the heating power supply of fermenting case by power-supply controller of electric.When being lower than, the temperature in the proving room sets when requiring, numeral shows that the heating power supply that thermostat is opened fermenting case by power-supply controller of electric continues to heat, this process circulation is repeatedly until arriving the fermentation time of setting, numeral shows that the time controller timing finishes, and the heating power supply that cuts off fermenting case by the power-supply controller of electric warning device prompting fermenting process that sounds simultaneously finishes.
This patent feature is: adopt the heating of two-way hot blast, can on a plurality of directions, heat, for example about, heat on the symmetry direction such as all around, preferably laterally vertically both direction heat; Adopt a plurality of ultraviolet sterilization lamps.The thermal source that is used for pasteurization is an electric heating tube, the hot blast heating, and the device that is used for the ultraviolet sterilization is the ultraviolet sterilization lamp pipe.Pasteurization and ultraviolet sterilization combination, sterilization and heat-preservation fermentation process are finished automatically.
The advantage of microbial fermentation case of the present invention: one-piece construction is simple, and cost of manufacture is cheap, and is easy to operate, and laterally vertically twocouese hot blast heat temperature raising is rapid, hot air circulation efficient height, temperature-stable, accurate, even; Sterilization, fermenting process can be finished automatically; Can produce multiple microorganism according to bacterial classification and fermentation condition different and satisfy different demands; A plurality of function components and parts can ensure the long-play of machine; Can be applicable to that southern and northern region carries out stably manufactured throughout the year.
The invention has the beneficial effects as follows: can finish sterilization automatically, the whole process of fermenting, simple in structure, easy to operate, cost of manufacture is cheap, and use range is extensive.The microorganisms producing flow process is few, and energy consumption is low, and cost is low, the number of viable height, and it is various to produce the viable bacteria kind, is highly suitable for plant's fermentative production high-activity microorganism.
Description of drawings:
The present invention is further described below in conjunction with drawings and Examples.
Fig. 1 is overall appearance figure of the present invention.
Fig. 2 is a front elevation of the present invention.
Fig. 3 is a longitudinal section of the present invention structural map.
Reference numeral lists as follows: 1, and casing; 2, numeral shows thermostat; 3, numeral shows time controller; 4, power-supply controller of electric; 5, warning device; 6, the temperature control probe; 7, ultraviolet germicidal; 8, fan; 9, dividing plate; 10, electrothermal tube; 11, the fermentation frame; 12, division plate.
Embodiment:
With reference to accompanying drawing 1, Fig. 2 and Fig. 3 explanation, be described as follows in conjunction with the embodiments: novel microorganism fermenting case of the present invention relates to casing 1, power-supply controller of electric 4, numeral demonstration thermostat 2, temperature control probe 6, numeral demonstration time controller 3, warning device 5, dividing plate 9, fan 8, electrothermal tube 10, ultraviolet germicidal 7, fermentation frame 11 and division plate 12.Described casing 1 can be the profile of vertical on the market or horizontal showcase.The said casing of the present invention is divided into three layers, and the upper strata is provided with the needed power-supply controller of electric 4 of fermenting case, numeral demonstration thermostat 2, numeral demonstration time controller 3 and warning device 5 for controlling layer.The power switch of power-supply controller of electric 4 control fermenting cases; Numeral shows that thermostat 2 shows the actual temperature of fermenting case and temperature is set; Numeral shows that time controller 3 shows the time of fermentation time and setting; Warning device 5 is used to point out temperature to surpass limiting temperature, timing end or fermentation ends.The middle layer is a proving room, is provided with temperature control probe 6 in the described proving room, and described temperature control probe 6 shows that with the numeral of controlling layer thermostat 2 is connected.Described proving room following and inboard be zone of heating, be provided with dividing plate 9 between proving room and the zone of heating.Respectively be provided with at least two ventilation holes 8 on the dividing plate, be provided with fan 8 in the ventilation hole.Be provided with electrothermal tube 10 in the zone of heating, be used to add warm air, heat is provided.Consider the work-ing life of component, in order not influence the works better of fermenting case, two covering devices are added in decision, ensure the machine long-play.Other structures all are provided with according to the prior art processes standard.
People are clearer to understand this fermenting case and use thereof in order to allow, be the method that fermenting case of the present invention is used in example explanation now: in proving room, put into Bacillus licheniformis and connect power supply after with substratum with fermentative production high reactivity Bacillus licheniformis (Bacilluslicheniformis), configure the needed temperature and time of microbial fermentation, the leavening temperature of Bacillus licheniformis is 35 ℃, and fermentation time is 24 hours.Said electrothermal tube 10 begins to heat up, and germicidal lamp 7 is started working, and the temperature in the zone of heating begins to rise, and from horizontal and vertical introducing proving room, do not stop to circulate guarantees temperature-stable, evenly in the proving room to the fan 8 on the dividing plate 9 with the hot blast of zone of heating.Sterilization process adopts pasteurization and ultraviolet sterilization combining method, and the pasteurization temperature is set to 65 ± 1 ℃, and sterilization time is 40 minutes.Consider that liquid has certain thickness, core temperature is raised to design temperature needs certain hour, delays time 10 minutes, and sterilization time begins to calculate from reaching 65 ℃.Set when requiring 65 ℃ when the temperature in the proving room reaches, numeral shows that thermostat 2 cuts off the heating power supply of fermenting cases by power-supply controller of electric 4.When being lower than, the temperature in the proving room sets when requiring, numeral shows that the heating power supply that thermostat 2 is opened fermenting case by power-supply controller of electric 4 continues to heat, this process circulation is repeatedly until arriving the fermentation time of setting, numeral shows that time controller 3 timing finish, heating unit 10 and ultraviolet germicidal 7 quit work automatically, finish whole sterilization process.Enter the temperature control program after sterilizing works is finished immediately, in order to cool the temperature to the temperature of suitable microbe inoculation bacterial classification, temperature is set at 35 ℃, and warning device 5 sounds and points out sterilizing program to finish behind the arrival design temperature.After sterilizing works is finished, the substratum temperature has dropped to suitable microbe inoculation bacterial classification, need the microbe inoculation bacterial classification this moment, with inoculation Bacillus licheniformis (Bacillus licheniformis) is example, in substratum, finish the secondary fermentation case and enter fermenting procedure automatically by inoculation with the Bacillus licheniformis bacterial classification inoculation for inoculum size according to 4%, and leavening temperature is set at 35 ℃, 24 hours heat-preservation fermentation time, warning device 5 sounded and points out whole fermentation process to finish after soaking time finished.The Bacillus licheniformis viable bacteria bacterium liquid that taking-up ferments can be applied to its feeding or environmental protection treatment immediately as required.
Leavening temperature and fermentation time can be changed according to the different of bacterial classification and fermentation condition, multiple different high-activity microorganism can be produced.The foregoing description only is that preferred implementation of the present invention is described; be not that design of the present invention and scope are limited; under the prerequisite of design concept of the present invention; any various modification and improvement that technical scheme of the present invention is made all should fall into protection scope of the present invention, and the technology contents that the present invention asks for protection is all charged in claims.

Claims (10)

1. novel microorganism fermenting case, comprise casing (1), control layer and proving room, be provided with temperature probe (6) in the proving room, it is characterized in that: a zone of heating is respectively arranged on many sides of proving room, be provided with dividing plate (9) between proving room and the zone of heating, dividing plate is provided with ventilation hole (8), is provided with fan (8) in the ventilation hole.
2. novel microorganism fermenting case according to claim 1 is characterized in that casing (1) is for vertical or horizontal.
3. novel microorganism fermenting case according to claim 1 is characterized in that being placed with in the proving room fermentation frame (11) and division plate (12).
4. novel microorganism fermenting case according to claim 1 is characterized in that being provided with in the zone of heating electrothermal tube (10).
5. novel microorganism fermenting case according to claim 1 is characterized in that controlling layer and comprises that the numeral that is connected with temperature probe (6) shows thermostat (2).
6. novel microorganism fermenting case according to claim 5 is characterized in that controlling layer and comprises that also power-supply controller of electric (4), numeral show thermostat (2), clock display control unit (3) and warning device (5).
7. novel microorganism fermenting case according to claim 1 is characterized in that thermal baffle is provided with at least two fans (8).
8. according to each described novel microorganism fermenting case among the claim 1-7, it is characterized in that being provided with in the proving room at least one ultraviolet germicidal (7).
9. according to each described microbial fermentation case in the claim 1 to 7, it is characterized in that the sterilization of fermenting case, fermenting process can finish automatically.
10. according to each described microbial fermentation case in the claim 1 to 8, it is characterized in that the sterilization method of fermenting case adopts pasteurization.
CN2010101291006A 2010-03-22 2010-03-22 Full-automatic microbial fermentation case for farm places and its application method Pending CN102199520A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108056492A (en) * 2018-02-02 2018-05-22 广州博善生物科技股份有限公司 Feed fermentation device

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2604439Y (en) * 2003-03-13 2004-02-25 魏福培 Double-protection thermostatic germiculture incubator
CN2631205Y (en) * 2003-07-07 2004-08-11 黑龙江龙泰生物技术开发有限公司 Apparatus for producing liquid mycelium of edible mushroom
CN2661697Y (en) * 2003-10-16 2004-12-08 谢守印 Multipurpose organism incubator
CN200952013Y (en) * 2006-09-30 2007-09-26 蒋衍恩 Constant temperature and constant humidity fermenting box
CN201420079Y (en) * 2009-05-19 2010-03-10 嘉兴市中新医疗仪器有限公司 Mould incubator
CN201869754U (en) * 2010-03-22 2011-06-22 段云峰 Bidirectional hot-air heating full-automatic microbial fermentation case used in culturing farm

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2604439Y (en) * 2003-03-13 2004-02-25 魏福培 Double-protection thermostatic germiculture incubator
CN2631205Y (en) * 2003-07-07 2004-08-11 黑龙江龙泰生物技术开发有限公司 Apparatus for producing liquid mycelium of edible mushroom
CN2661697Y (en) * 2003-10-16 2004-12-08 谢守印 Multipurpose organism incubator
CN200952013Y (en) * 2006-09-30 2007-09-26 蒋衍恩 Constant temperature and constant humidity fermenting box
CN201420079Y (en) * 2009-05-19 2010-03-10 嘉兴市中新医疗仪器有限公司 Mould incubator
CN201869754U (en) * 2010-03-22 2011-06-22 段云峰 Bidirectional hot-air heating full-automatic microbial fermentation case used in culturing farm

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108056492A (en) * 2018-02-02 2018-05-22 广州博善生物科技股份有限公司 Feed fermentation device

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Application publication date: 20110928