CN102198293A - Compound type chitin artificial skin and preparation method thereof - Google Patents
Compound type chitin artificial skin and preparation method thereof Download PDFInfo
- Publication number
- CN102198293A CN102198293A CN2011101391511A CN201110139151A CN102198293A CN 102198293 A CN102198293 A CN 102198293A CN 2011101391511 A CN2011101391511 A CN 2011101391511A CN 201110139151 A CN201110139151 A CN 201110139151A CN 102198293 A CN102198293 A CN 102198293A
- Authority
- CN
- China
- Prior art keywords
- chitin
- artificial skin
- solution
- water
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention provides compound type chitin artificial skin. In the compound type chitin artificial skin, a water-soluble chitin membrane is used as a skin stent, and a layer of chitin powder is compounded on the chitin membrane, wherein the chitin membrane is made of chitin solution which takes water as a solvent, and a ratio of the chitin to the chitin powder to the water (w/w/v) is (100-250 mg):(1,000-1,500 mg):10ml. The compound type chitin artificial skin has the softness, elasticity and certain mechanical strength of thin films, has the advantages of no toxicity, side effect, irritation, rejection, pyrogenicity or sensitization of the chitin powder, has high compatibility and affinity with tissues of human bodies, can be absorbed by the tissues of the human bodies quickly, and does not have scabs and scars.
Description
Technical field
The invention belongs to medicine, biological technical field, relate to the preparation method of medical accessory, relate in particular to a kind of compound chitin artificial skin and preparation method thereof.
Background technology
Chitin (chitin) is a kind of natural polysaccharide, from carapaces such as arthropod such as shrimp, Eriocheir sinensis, insecticide, extract, it can promote epithelial growth and the growth that suppresses antibacterial, the compatibility of this class natural polysaccharide and tissue is good,, non-immunogenicity nontoxic, non-stimulated to human body, therefore extremely people pay attention to.
At present, existing about utilizing chitin or chitin to manufacture the reported in literature of artificial skin, product about artificial skin on the market also has a variety of, the artificial skin made from chitin is the very strong tissue of a kind of regeneration capacity, the inhibiting bacteria and diminishing inflammation effect is arranged, can be used for multiple wound healing, healing along with wound face, self skin also can be grown, and chitin dissolves voluntarily and is absorbed by the body, avoided taking off dressing and produced the situation of bleeding and causing patient's misery, it has extremely important medical effect.But the raw material that chitin artificial skin commonly used is now adopted is generally the chitin of deacetylation; perhaps carboxylated chitin; and to use acid, alkali or organic solvent dissolution, immersion or flushing; material composition is complicated; operation sequence is also loaded down with trivial details, and also there are defectives such as relatively poor, the active little and quality of water absorbing properties is harder in the artificial skin that makes.
Summary of the invention
It is poor to the present invention is directed to the water absorbing properties that the chitin artificial skin exists in the prior art, activity diminishes and harder deficiency, a kind of compound chitin artificial skin and preparation method thereof is provided, promptly with the water-soluble chitosan film as dermal scaffold, compound last layer chitin powder on chitin film, the flexibility of the existing thin film of product of the present invention, elasticity and certain mechanical strength, the nontoxic of chitin powder arranged again, have no side effect, nonirritant, do not reject, pyrogenicity not, the advantage of sensitization not, it and tissue have the fabulous compatibility and affinity, can be by the very fast absorption of tissue, thereby do not form a scab no cicatrix.
For achieving the above object, the present invention adopts following technical proposals to be achieved:
A kind of compound chitin artificial skin, it is characterized in that it is made by compound one deck chitin powder on one deck chitin film, described chitin film is made by the chitin solution that with water is solvent, described chitin: chitin powder: water (w/w/v)=(100mg-250mg): (1000mg-1500mg): 10ml.
Further improvement to technical scheme: described chitin solution is that the water-soluble chitosan of 300 lis of handkerchief-1000 li handkerchiefs is water-soluble formulated by viscosity, and the mass volume ratio concentration of described chitin solution is 1%-2.5%.
Further improvement to technical scheme: described chitin powder is the chitin powder of 300 lis of handkerchief-1000 li handkerchiefs of viscosity.
The present invention also provides a kind of preparation method of compound chitin artificial skin, and it may further comprise the steps:
(1) preparation of chitin solution
Elder generation's dose volume is than being the glycerine water solution of glycerol: water=1:2, be that solvent preparation mass volume ratio is the chitin solution of 1%-2.5% again with the distilled water, with described glycerine water solution and chitin solution according to (100ml-200ml): be mixed with chitin solution behind the volume ratio mix homogeneously (800ml-1000ml);
(2) preparation of chitin film
Take by weighing the described chitin solution 12ml-15ml that step (1) makes, divide on the flat board of cleaning, making it to form area is 120cm
2-150cm
2The round pie thin film, thickness is 0.1mm-0.2mm, forms chitin film behind air drying 18-36 hour of cleaning;
(3) preparation of compound chitin artificial skin
Brushing one deck mass volume ratio on described chitin film is the chitin solution of 1%-2.5%, then the chitin powder of 1g-1.5g is dispersed on the described chitin film uniformly, on chitin powder, add glass plate and weight compresses, remove glass plate and weight after 1-3 hour, air drying 10-18 hour of cleaning;
(4) ultraviolet sterilization
Then compound chitin artificial skin is placed under the ultraviolet radiation, turn again after half an hour and shine half an hour, take out standby.
Further improvement to technical scheme: weight is the weight of 1-3Kg in the described step (3).
Compared with prior art, advantage of the present invention and good effect are:
1, artificial skin of the present invention has good flexibility and elasticity, has certain mechanical strength simultaneously, can seal wound face, is fit to the requirement of artificial skin.
2, artificial skin of the present invention the contact wound one side be chitin powder, its physical property and biological activity all do not change, kept chitin or chitin nontoxic, have no side effect, not pyrogenicity, non-sensitization, do not reject and non-stimulated advantage.
3, artificial skin of the present invention can quick-acting haemostatic powder, pain relieving, antibacterial, antiinflammatory, and promote the regeneration of skin.
4, the biocompatibility of artificial skin of the present invention and tissue and affinity are all fine, can be by after the tissue, thereby can not form a scab, can not stay cicatrix, be the ideal material of artificial skin.
The specific embodiment
Below in conjunction with the specific embodiment technical scheme of the present invention is described in further detail.
Embodiment 1
The concrete compound method of artificial skin of the present invention is as follows:
1, the preparation of chitin solution
Take by weighing glycerol 50ml and distilled water 100ml mix homogeneously earlier, preparation becomes glycerine water solution, getting middle and high viscosity water-soluble chitosan 25g is dissolved among the distilled water 825ml, the viscosity of described chitin is 300 lis of handkerchief-1000 li handkerchiefs, described glycerine water solution 150ml poured in the chitin solution that makes both are mixed, be mixed with chitin solution behind the mix homogeneously, standby.
The present invention mixes glycerine water solution with chitin solution, utilize glycerine water solution be difficult for to do, easily with skin attachement and have the characteristics of flexibility, make the chitin artificial skin also have soft and be difficult for advantages such as dried.
2, the preparation of chitin film
Take by weighing described chitin solution 12-15ml, divide on the glass plate of cleaning, making it to form area is 120cm
2The round pie thin film, chitin film is natural drying in drying device, use earlier air purifier with the drying device inner air-cleaning after, again chitin film is put into drying device, promptly form the chitin thin film after dry 24 hours.
3, the preparation of compound chitin artificial skin
Brushing one deck mass volume ratio with the small brushes of cleaning on described chitin film is 1% chitin solution, then the chitin powder of 1g is dispersed on the described chitin film uniformly.Be pressed on the chitin powder in addition and also compress about weighting material 1Kg, remove weight and glass plate after about 2 hours, the air drying of cleaning 12 hours with the glass plate of a cleaning.
4, ultraviolet radiation sterilization
The dried composite membrane to seven, eighty per cant is moved in the stainless steel disc carefully, turn again after half an hour with ultraviolet radiation and shine half an hour.
5, vacuum-packed, standby.
Artificial skin of the present invention is used for clinical trial as medical artificial skin, and result of the test is as follows:
1, in May, 2010,5 years old boy Gao Jun throw from horizontal bar, the inside and outside long wound of 3cm of all having an appointment of chin, dark about 2-2.5mm, apply at inside and outside wound with product of the present invention immediately after after sterilizing, pain falls sharply after about one minute, hemostasis after about 3 minutes, wound is almost recovered after one night, still normally goes up kindergarten (Nanjing Road, Qingdao City first kindergarten) in second day.
2, in July, 2010,5 years old boy Gao Jun mechanically throws from walking, and wound site, size are almost identical with last time, also is night recovery from illness after same the processing, do not stay cicatrix.
3, in August, 2000, Nanchang computer work person Zhang Gang, 40 years old, because of falling down the scratch ancon by motorcycle, the about 6 * 4cm of area applied product of the present invention after sterile-processed, found wound tissue regeneration promoting skin and chitin is vanished from sight when checking after 24 hours.
4, in October, 2000, the worker of Nanchang chemical engineering school explains new hall, difficulty, and 50 years old, being burnt by NaOH in the ankle below, has a skin of silver dollar size to be corroded, and through applying product of the present invention with tap water after a large amount of flushings, recovery from illness in second day does not stay cicatrix.
Above embodiment is only in order to illustrating technical scheme of the present invention, but not limits it; Although the present invention is had been described in detail with reference to previous embodiment, for the person of ordinary skill of the art, still can make amendment to the technical scheme that previous embodiment is put down in writing, perhaps part technical characterictic wherein is equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution break away from the spirit and scope of the present invention's technical scheme required for protection.
Claims (5)
1. compound chitin artificial skin, it is characterized in that it is made by compound one deck chitin powder on one deck chitin film, described chitin film is made by the chitin solution that with water is solvent, described chitin: chitin powder: water (w/w/v)=(100mg-250mg): (1000mg-1500mg): 10ml.
2. a kind of compound chitin artificial skin according to claim 1, it is characterized in that described chitin solution is that the water-soluble chitosan of 300 lis of handkerchief-1000 li handkerchiefs is water-soluble formulated by viscosity, the mass volume ratio concentration of described chitin solution is 1%-2.5%.
3. a kind of compound chitin artificial skin according to claim 1 is characterized in that described chitin powder is the chitin powder of 300 lis of handkerchief-1000 li handkerchiefs of viscosity.
4. the preparation method of a compound chitin artificial skin is characterized in that it may further comprise the steps:
(1) preparation of chitin solution
Elder generation's dose volume is than being the glycerine water solution of glycerol: water=1:2, be that solvent preparation mass volume ratio is the chitin solution of 1%-2.5% again with the distilled water, with described glycerine water solution and chitin solution according to (100ml-200ml): be mixed with chitin solution behind the volume ratio mix homogeneously (800ml-1000ml);
(2) preparation of chitin film
Take by weighing the described chitin solution 12ml-15ml that step (1) makes, divide on the flat board of cleaning, making it to form area is 120cm
2-150cm
2The round pie thin film, thickness is 0.1mm-0.2mm, forms chitin film behind air drying 18-36 hour of cleaning;
(3) preparation of compound chitin artificial skin
Brushing one deck mass volume ratio on described chitin film is the chitin solution of 1%-2.5%, then the chitin powder of 1g-1.5g is dispersed on the described chitin film uniformly, on chitin powder, add glass plate and weight compresses, remove glass plate and weight after 1-3 hour, air drying 10-18 hour of cleaning;
(4) ultraviolet sterilization
Then compound chitin artificial skin is placed under the ultraviolet radiation, turn again after half an hour and shine half an hour, take out standby.
5. the preparation method of a kind of compound chitin artificial skin according to claim 4 is characterized in that weight is the weight of 1-3Kg in the described step (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101391511A CN102198293A (en) | 2011-05-27 | 2011-05-27 | Compound type chitin artificial skin and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101391511A CN102198293A (en) | 2011-05-27 | 2011-05-27 | Compound type chitin artificial skin and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102198293A true CN102198293A (en) | 2011-09-28 |
Family
ID=44659446
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101391511A Pending CN102198293A (en) | 2011-05-27 | 2011-05-27 | Compound type chitin artificial skin and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102198293A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116285018A (en) * | 2023-02-13 | 2023-06-23 | 武汉轻工大学 | Method for enhancing tensile property of squid cartilage, squid cartilage film and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0596215A1 (en) * | 1992-11-02 | 1994-05-11 | Sunfive Company Ltd | Applying material for protecting wound surface |
| CN1485099A (en) * | 2003-08-22 | 2004-03-31 | 刘永庆 | Preparation process for artificial skin |
| CN1500525A (en) * | 2002-11-12 | 2004-06-02 | 吴奕光 | Medical dressing and its producing method |
| CN101284145A (en) * | 2008-04-21 | 2008-10-15 | 武汉锐尔生物科技有限公司 | Medical dressing and its preparation method and application |
-
2011
- 2011-05-27 CN CN2011101391511A patent/CN102198293A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0596215A1 (en) * | 1992-11-02 | 1994-05-11 | Sunfive Company Ltd | Applying material for protecting wound surface |
| CN1500525A (en) * | 2002-11-12 | 2004-06-02 | 吴奕光 | Medical dressing and its producing method |
| CN1485099A (en) * | 2003-08-22 | 2004-03-31 | 刘永庆 | Preparation process for artificial skin |
| CN101284145A (en) * | 2008-04-21 | 2008-10-15 | 武汉锐尔生物科技有限公司 | Medical dressing and its preparation method and application |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116285018A (en) * | 2023-02-13 | 2023-06-23 | 武汉轻工大学 | Method for enhancing tensile property of squid cartilage, squid cartilage film and application |
| CN116285018B (en) * | 2023-02-13 | 2024-06-07 | 武汉轻工大学 | Method for enhancing tensile property of squid cartilage, squid cartilage film and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103520764B (en) | Functional dressing, and preparation method and application thereof | |
| RU2707254C1 (en) | Aqueous pharmaceutical preparation and method of treating chronic ulcer | |
| Zhou et al. | Rational design of intelligent and multifunctional dressing to promote acute/chronic wound healing | |
| EP3305335A1 (en) | Multifunctional compound skin or wound dressing as regenerative skin substitute | |
| Thomas | Surgical dressings and wound management | |
| US20130251665A1 (en) | Treatment of chronic ulcerous skin lesions | |
| JP5460580B2 (en) | Novel active ingredients and their use in fusion | |
| Hasatsri et al. | Randomized Clinical Trial of the Innovative Bilayered Wound Dressing Made of Silk and Gelatin: Safety and Efficacy Tests Using a Split‐Thickness Skin Graft Model | |
| MX2008000969A (en) | Biomaterials based on carboxymethylcellulose salified with zinc associated with hyaluronic acid derivatives. | |
| MX2012009675A (en) | Use of synthetic polysulphated oligosaccharides as cleaning agents for a wound. | |
| JP6212552B2 (en) | Dressing material that releases active ingredients continuously | |
| JP2527132B2 (en) | Topical drug composition comprising hydrophilic polysaccharide | |
| Vachhrajani et al. | Science of wound healing and dressing materials | |
| Ajiteru et al. | Fabrication and characterization of a myrrh hydrocolloid dressing for dermal wound healing | |
| CN101658690A (en) | Officinal volatile argyi leaf oil dressing and preparation method thereof | |
| DE60312969T2 (en) | PREPARATION FOR WOUND HEALING AND PREVENTING THE POSSESSION OF ASSOCIATIONS TO THE WOUNDS | |
| CN110876814A (en) | Liquid band-aid rich in antibacterial peptide and preparation method thereof | |
| US20060120993A1 (en) | Treatment of lesions of the soft tissues | |
| CN105411746A (en) | Nanometer multilayer composite hemostasis plaster, and preparing method and application thereof | |
| CN102198293A (en) | Compound type chitin artificial skin and preparation method thereof | |
| US20090148394A1 (en) | Treatment of chronic ulcerous skin lesions | |
| WO2016080158A1 (en) | Biological film preparation for promoting wound healing and coating and protecting biological organs | |
| Son et al. | Preparation and evaluation of Annona glabra L. leaf extract contained alginate film for burn healing | |
| CN109432483A (en) | A kind of medical dressing and its preparation method and application accelerating wound healing | |
| KR20150081844A (en) | Method for preparing hydrocolloid membrane containing Styela clava tunic |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20110928 |