CN102197047B - 突变的δ5去饱和酶及其在制备多不饱和脂肪酸中的用途 - Google Patents

突变的δ5去饱和酶及其在制备多不饱和脂肪酸中的用途 Download PDF

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CN102197047B
CN102197047B CN200980136806.0A CN200980136806A CN102197047B CN 102197047 B CN102197047 B CN 102197047B CN 200980136806 A CN200980136806 A CN 200980136806A CN 102197047 B CN102197047 B CN 102197047B
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desaturase
seq
acid
host cell
mutant
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Expired - Fee Related
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CN200980136806.0A
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Chinese (zh)
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CN102197047A (zh
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Q·朱
D·M·W·波拉克
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200980136806.0A 2008-09-19 2009-09-18 突变的δ5去饱和酶及其在制备多不饱和脂肪酸中的用途 Expired - Fee Related CN102197047B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US9833308P 2008-09-19 2008-09-19
US61/098,333 2008-09-19
US61/098333 2008-09-19
PCT/US2009/057393 WO2010033753A2 (en) 2008-09-19 2009-09-18 Mutant delta5 desaturases and their use in making polyunsaturated fatty acids

Publications (2)

Publication Number Publication Date
CN102197047A CN102197047A (zh) 2011-09-21
CN102197047B true CN102197047B (zh) 2014-07-09

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CN200980136806.0A Expired - Fee Related CN102197047B (zh) 2008-09-19 2009-09-18 突变的δ5去饱和酶及其在制备多不饱和脂肪酸中的用途

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US (3) US8268598B2 (cg-RX-API-DMAC7.html)
EP (1) EP2324119B1 (cg-RX-API-DMAC7.html)
JP (1) JP5762291B2 (cg-RX-API-DMAC7.html)
CN (1) CN102197047B (cg-RX-API-DMAC7.html)
AU (1) AU2009293160B2 (cg-RX-API-DMAC7.html)
BR (1) BRPI0913714A2 (cg-RX-API-DMAC7.html)
CA (1) CA2735379A1 (cg-RX-API-DMAC7.html)
CL (1) CL2011000560A1 (cg-RX-API-DMAC7.html)
DK (1) DK2324119T3 (cg-RX-API-DMAC7.html)
WO (1) WO2010033753A2 (cg-RX-API-DMAC7.html)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8268598B2 (en) * 2008-09-19 2012-09-18 E I Du Pont De Nemours And Company Mutant Δ5 desaturases mutated in the heme-binding motif (HPGG) and their use in making polyunsaturated fatty acids
CA2795460A1 (en) 2010-04-22 2011-10-27 E. I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
US20140093910A1 (en) * 2010-06-10 2014-04-03 Bengurion University Of The Negev Research And Development Authority D5 desaturase-defective mutant gene and use thereof
AU2011293180B2 (en) 2010-08-26 2017-03-02 E. I. Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
US8658413B2 (en) 2010-08-26 2014-02-25 E I Du Pont De Nemours And Company Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids
KR20140008295A (ko) 2010-08-26 2014-01-21 이 아이 듀폰 디 네모아 앤드 캄파니 돌연변이 델타-9 연장효소 및 다중불포화 지방산의 제조에서의 그들의 용도
US8735137B2 (en) 2010-12-30 2014-05-27 E I Du Pont De Nemours And Company Use of Saccharomyces cerevisiae SUC2 gene in Yarrowia lipolytica for sucrose utilization
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
CA3026436A1 (en) 2011-02-11 2012-08-16 E. I. Du Pont De Nemours And Company Method for obtaining a lipid-containing composition from microbial biomass
CA2877279A1 (en) * 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company Improved production of polyunsaturated fatty acids by coexpression of acyl-coa:lysophosphatidylcholine acyltransferases and phospholipid:diacylglycerol acyltransferases
WO2014074770A2 (en) 2012-11-09 2014-05-15 Heliae Development, Llc Balanced mixotrophy methods
WO2014074772A1 (en) 2012-11-09 2014-05-15 Heliae Development, Llc Mixotrophic, phototrophic, and heterotrophic combination methods and systems
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
EP2935591B1 (en) 2012-12-21 2017-07-05 E. I. du Pont de Nemours and Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
BR112015020827A2 (pt) * 2013-02-28 2017-10-10 Euglena Co Ltd método para a introdução de gene em euglena, e transformante do mesmo
JP2014193154A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナの形質転換体
JP2014193153A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナへの遺伝子導入方法
EP4049537A1 (en) 2018-12-12 2022-08-31 Nippon Suisan Kaisha, Ltd. A composition containing highly unsaturated fatty acid or alkyl ester thereof and a method for producing the same

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070271632A1 (en) * 2006-05-17 2007-11-22 Howard Glenn Damude Delta 5 desaturase and its use in making polyunsaturated fatty acids
US20080155705A1 (en) * 2003-08-01 2008-06-26 Basf Plant Science Gmbh Method For the Production of Multiple-Unsaturated Fatty Acids in Transgenic Organisms

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5972664A (en) 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
CN101970638B (zh) 2007-10-03 2015-02-11 纳幕尔杜邦公司 用于高水平生产二十碳五烯酸的优化解脂耶氏酵母菌株
US8268598B2 (en) * 2008-09-19 2012-09-18 E I Du Pont De Nemours And Company Mutant Δ5 desaturases mutated in the heme-binding motif (HPGG) and their use in making polyunsaturated fatty acids

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080155705A1 (en) * 2003-08-01 2008-06-26 Basf Plant Science Gmbh Method For the Production of Multiple-Unsaturated Fatty Acids in Transgenic Organisms
US20070271632A1 (en) * 2006-05-17 2007-11-22 Howard Glenn Damude Delta 5 desaturase and its use in making polyunsaturated fatty acids

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
The alternative pathway C20 D8-desaturase from the non-photosynthetic organism Acanthamoeba castellanii is an atypical cytochrome b5-fusion desaturase.;SAYANOVA等;《FEBS》;20061231;第580卷;1946-1952 *
肖靓等.实时PCR分析Δ5脱饱和酶在花生四烯酸合成中的作用.《中国生物工程杂志》.2006,第26卷(第8期),57-61. *

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Publication number Publication date
DK2324119T3 (en) 2016-07-18
WO2010033753A2 (en) 2010-03-25
US8268598B2 (en) 2012-09-18
EP2324119A2 (en) 2011-05-25
BRPI0913714A2 (pt) 2020-10-13
EP2324119A4 (en) 2013-05-01
US20120309064A1 (en) 2012-12-06
US8367383B2 (en) 2013-02-05
CL2011000560A1 (es) 2011-10-07
CA2735379A1 (en) 2010-03-25
JP2012502659A (ja) 2012-02-02
WO2010033753A3 (en) 2010-05-27
JP5762291B2 (ja) 2015-08-12
US20130122558A1 (en) 2013-05-16
US8614075B2 (en) 2013-12-24
CN102197047A (zh) 2011-09-21
AU2009293160A1 (en) 2010-03-25
AU2009293160B2 (en) 2013-11-21
EP2324119B1 (en) 2016-03-30
US20100075386A1 (en) 2010-03-25

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