CN102190523A - Nano biofertilizer production technology and preparation method - Google Patents
Nano biofertilizer production technology and preparation method Download PDFInfo
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- CN102190523A CN102190523A CN2010101255531A CN201010125553A CN102190523A CN 102190523 A CN102190523 A CN 102190523A CN 2010101255531 A CN2010101255531 A CN 2010101255531A CN 201010125553 A CN201010125553 A CN 201010125553A CN 102190523 A CN102190523 A CN 102190523A
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Abstract
The invention discloses a nano biofertilizer production technology and a nano biofertilizer preparation method. The invention comprises the following steps of: mixing nano biochemical materials, a yeast fermentation broth extract and macromolecular natural organic compounds for reaction, grinding a crude toosedarin product treated by bacillus thuringiensis, bacillus subtilis, trichoderma viride and P.chrysosporium mixed fermentation liquor, and mixing; and adding amide compounds and ammonium nitrogen compounds in turn to obtain the nano biofertilizer. The fertilizer has the effects of improving effective utilization rate of N, P and K by 30 percent and reducing loss by 50 percent, can accelerate propagation of microbes beneficial for soil, improve the activity of related enzyme systems, comprehensively improve the soil fertility, improve soil environmental condition, facilitate improving quality of crop products and improve the disease resistance of crops, has effects of killing insects, reducing consumption of pesticides and the like to some extent, greatly reduces pollution sources and has obvious functions of reducing cost and the like.
Description
Technical field:
The invention belongs to a kind of nano biological fertilizer production technology.Specifically be that nano meter biomaterial is made an addition among the fertilizer, and a kind of novel nano biological fertilizer of making.
Background technology:
Nanotechnology now in a lot of fields, particularly military, communication and electronic technology have obtained rapid progress.Scientist is to the research of nano material, mainly based on metal and inorganic nonmetallic nanometer material (accounting for more than 80%), and several in the research and development of nano biological, organic nano and agricultural chemicals nano material near zero.Therefore, nano biological fertilizer is a kind of novel concept and high-tech product.At present the whole world does not have product to go on the market as yet, domesticly then just begin one's study, but also product-free comes out.So the research and development of nano biological fertilizer is significant.
According to incompletely statistics, in recent years, world developed country all is used as science and the engineering field that will most possibly make a breakthrough in future to nanosecond science and technology.In this hi-tech that is the theme with nanometer war, Japan will drop into 24,000,000,000,000 yen at 5 years from now on. be used for the research of nanotechnology and life science, information technology etc.; The U.S. has formulated new millennium country's nanotechnology strategy. and give 4.97 hundred million dollars of this program appropriations; The policy of Commissariat A L'Energie Atomique also shifts to nanotechnology rapidly, and playing an active part in of various countries shifted this hi-tech fight onto perfervid degree.But the interior in a lot of fields development of nanotechnology is very uneven.The nano material class accounts for 80.8% according to statistics; Electrical type 2.7%; Medicine 4%; Other class 12.5%.Nanometer is in agrotechnique, and particularly the agrochemistry aspect is almost nil.
Recently, many expert's prophesies, the influence of nanotechnology will be above robot calculator (computer).Therefore, they advise that nanotechnology should be in information material, biomaterial and three field fast developments of energy and material, and should strengthen the input of fund and technology, so just can catch up with the trend in science and technology epoch.
The nanotechnology industry of China mainly contains weaving, plastics, pottery, coating, rubber etc. in the application facet of daily necessities, but is used for the surface modification of product more.Wherein specific product has nano-cloth, nano plastic, nano-glass, nano ceramics, nanometer water and nanometer oil.This shows that nanotechnology and industrialization thereof just more and more are subjected to the attention of various circles of society in recent years.
Many insiders and expert advice: have the courage and tell the public truth, cultivate rational human consumer, allow them on the basis of understanding, play an active part in, allow " nanometer " really to warm up, form rational nanometer and pay close attention to atmosphere.Under the public's playing an active part in, positively perfect, development nanotechnology.In China, the Application and Development of nanotechnology is relatively backward, mostly still in experimental stage.Therefore should eliminate notional obstacle.Someone always takes the situation of applicable cases cover China of external advanced country, thinks external and do not break through that we just can not break through; External do not use, we also can not use, thereby suspect, negate our product.Nanotechnology is new technical field in fact, and everybody is ground zero, and gap is little, and the nanometer industry of China needn't be made a humble estimate of one's abilities. fully can be synchronous with the world. in addition leading.2007, China listed nanotechnology in one of four big important scientific research contents.
In the Nano-technology Development process, expert, scholar also want tolerant 1 unapprehended thing not to be eager to draw a conclusion, because may all become new technology standards of measurement and idea.The entrepreneur will have thinking and patient.Not eager for instant success and quick profits.More need not " be used and get nowhere " institute's yoke by present what is called.To begin industrialization from basic application one step at a time, pay close attention to its development and influence simultaneously coming.Go up bold imagination has been made in the development of Mirae Nano Technologies Co., Ltd. in " commercial and science forum " as the U.S. professor invited specially of Ka Naijimailong university Allan Na Kailun.He points out " we be jumping the Nano-technology Development of moving towards new in addition might surpass the influence of computer to the human lives ".Therefore, we should make great efforts to develop this emerge science, and have breakthrough on gordian technique, make product move towards market.
The present invention is a kind of product of nano biological fertilizer hi-tech.It is to utilize biology and Measurement for Biochemistry, and the macromole natural organic-compound is degraded to micromolecular compound, ion and atom.And its size is controlled between the 80-100nm (nanometer).Then with this class nano meter biomaterial, make an addition in the fertilizer or the part base-material of as fertilizer sources.The nano biological fertilizer that makes with this technology has following characteristics:
1, the utilization ratio of the common fertilizer that nano biological fertilizer is more equal than nutritive element improves more than 30%; The nitrogen loss rate has reduced more than 50%.Control the speed that organic nitrogen (N) decomposes significantly, reduced the loss of N.Significantly reduced to the eutrophication of edatope with to atmospheric pollution.
2, nano biological fertilizer has the organic pollutant of eliminating in the soil, makes its degraded or decomposition and disappears.Studies show that this product can reach 80%, 85% and 90% respectively to the disappearance rate of toxic compounds in 30-60 days such as many agricultural chemicals such as phenyl-hexachloride, DDT, aldrins.
3, nano biological fertilizer contains through two kinds of beneficial bacterias of screening and four kinds of organized enzymes that produced thereof.Thereby improved the ability of biological restoration effectively.Four kinds of organized enzymes have: catalase, laccase, tyrosine oxidase and hydroamidase.
4, above-mentioned first three kind enzyme plays a leading role in decontamination and biological restoration process.Particularly to deleterious organic pollutant such as Ppolynuclear aromatic hydro carbons (PAHs), polychlorinated biphenyl class (PCBs) and polychlorostyrene naphthoquinones class compounds such as (PCNs).A kind of organized enzyme in back then is the plain important enzyme systems of regulation and control plant nitrogen (N).It mainly regulates and control the organic amide compounds, promptly activity should be arranged. control active speed (organic nitrogen is slowly discharged) again.Therefore, there is a kind of nano meter biomaterial just can reach this requirement.For example 1 part of amidohpdrolase enzyme can decompose 500,000 parts acid amides, as methane amide, promptly is commonly called as urea, thereby causes a large amount of losses (rate of loss can up to 60%) of available nitrogen, has so both caused the pollution to environment, has wasted fertilizer again.
5, nano biological fertilizer also contains plant toosedarin (chinaberry element), is to have the function composition that prevents disease and pest.Can prevent and treat the harm of hundreds of kind insect and multiple pathogenic agent.
6, the enzyme reaction formula in the nano biological fertilizer has following two classes:
Participate in the representative formula of biological restoration reaction:
Participate in the general formula of amide hydrolysis:
R·CONH
2+H
2O→NH
3+R·COOH (2)
Participate in the reaction formula of carboxamide:
NH
2CONH
2+H
2O→CO
2+2NH
3 (3)
Participate in the general formula of kinetic reaction:
H
2N-C one enzyme (4)
Nano biological fertilizer is to reaction formula (1), (2) tool promoter action.
Nano biological fertilizer had both been had a promoter action to reaction formula (3), (4), again it was played certain restraining effect.Under certain condition, big as the pH value, when temperature was high again, main effect should be based on restraining effect.
Summary of the invention:
The major ingredient of nano biological fertilizer of the present invention is:
1, nano biological chemical material lytic enzyme and lignin polysaccharide macro-molecular lytic enzyme are a kind of nano materials that can fast the macromole natural organic matter be degraded to micromolecular compound, ion and atom.
2, two kinds of bacteriums, Bacillus thuringiensis (Bacillus Thuringiensis) and subtilis (BacillusSubtilis).Two kinds of fungies are viride (T.Viride) and the flat lead fungi of golden spore (P.Chrysosporium).
Above microorganism can produce under specific combination and environment: four kinds of organized enzymes such as catalase, laccase, tyrosine oxidase and hydroamidase.These organized enzymes are eliminated in toxic organic compound pollution and the biological restoration process in degraded and are played a leading role.
3, yeast fermentation liquid extract.
4, macromole natural organic-compound---leached tea oil slag and soybean meal etc.
5, plant nitrogen nutrition thing---amides (being mainly l-asparagine, paddy hydrogen acid amides and carboxamide) and a spot of ammonium nitrogen compound.
6, plant toosedarin (compound). mainly contain alkaloid and nonprotein amino acid etc.
Specific embodiments:
The ratio of various raw materials (%) in the nano biological fertilizer:
1, nano biological chemical material: 6%
2, microbial inoculum (containing two kinds of bacteriums and two kinds of fungies) 8%
3, macromole natural organic platform thing
(leached tea oil slag or soybean meal) 25%
Agricultural crop straw 21%
4, amides 20%
5, ammonium nitrogen compound (comprising part phosphorus and potassium compound) 8%
6, yeast fermentation liquid extract (crude extract) 7%
7, plant toosedarin (raw product) 5%
Total amount 100%
The total nutrient of nano biological fertilizer 〉=22%, organic 〉=30%, moisture≤10%, pH 6.6-7.5, effectively bacterium number 〉=0.2 hundred million/gram.
The preparation method is: after at first the macromole natural organic-compound being pulverized through pulverizing mill, add nano biological chemical material and yeast fermentation liquid extract, carried out in cell therefor solid reaction 5-7 days, temperature is controlled at below 60 degree.Plant toosedarin raw product with the mixed fermentation of process microbial inoculum mixes then, and stacks 3-5 days under 32 degree temperature.Add quantitative amides and the abundant mix of ammonium nitrogen compound at last, pass through granulation again, cryodrying promptly becomes nano biological fertilizer material finished product, also can pack to come into the market to sell.
Claims (8)
1. nano biological fertilizer, be by macromole natural organic-compound, plant toosedarin compound, four kinds of organized enzymes of two kinds of fungi microbes of two kinds of bacteriums, yeast fermentation broth and nano biological chemical material, through behind the solid fermentation again with amides, the nano biological solid fertilizer that the ammonium nitrogen compound forms by specific mixed.
2. the macromole natural organic-compound is leached tea oil slag, soybean meal and agricultural crop straw in the described nano biological fertilizer of claim 1.
3. two kinds of bacteriums are in the described nano biological fertilizer of claim 1: Bacillus thuringiensis (BacillusThuringiensis), subtilis (Bacillus Subtilis), two kinds of fungies are viride (T.Viride) and the flat lead fungi of golden spore (P.Chrysosporium), (T.Viriae).
4. four kinds of organized enzymes are in the described nano biological fertilizer of claim 1: catalase, laccase, tyrosine oxidase and hydroamidase.
5. amides is in the described nano biological fertilizer of claim 1: l-asparagine, paddy hydrogen acid amides and carboxamide.
6. plant toosedarin compound is in the described nano biological fertilizer of claim 1: alkaloid and nonprotein propylhomoserin.
7. the nano biological chemical material is in the described nano biological fertilizer of claim 1: nano biological lytic enzyme and xylogen polysaccharide macro-molecular lytic enzyme.
8. the described 4 kinds of microbial strainss of claim 3 can contain 4 kinds of microbial strainss simultaneously, also can contain wherein 2-3 kind arbitrarily.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010101255531A CN102190523A (en) | 2010-03-17 | 2010-03-17 | Nano biofertilizer production technology and preparation method |
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| CN2010101255531A CN102190523A (en) | 2010-03-17 | 2010-03-17 | Nano biofertilizer production technology and preparation method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104447125A (en) * | 2014-12-16 | 2015-03-25 | 河北省微生物研究所 | High lysine-rich fertilizer special for corn and preparation method of high lysine-rich fertilizer |
| CN108739109A (en) * | 2018-05-31 | 2018-11-06 | 贵州盛达生植物发展有限公司 | A kind of cultural method of dendrobium candidum |
| CN111393224A (en) * | 2018-12-28 | 2020-07-10 | 中科实盈(广东)生物技术有限公司 | Method and apparatus for producing nano fertilizer and other types of solid and liquid organic-inorganic compound fertilizer and slow-release pesticide |
-
2010
- 2010-03-17 CN CN2010101255531A patent/CN102190523A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104447125A (en) * | 2014-12-16 | 2015-03-25 | 河北省微生物研究所 | High lysine-rich fertilizer special for corn and preparation method of high lysine-rich fertilizer |
| CN104447125B (en) * | 2014-12-16 | 2017-04-05 | 河北省微生物研究所 | High-lysine corn special fertilizer and preparation method thereof |
| CN108739109A (en) * | 2018-05-31 | 2018-11-06 | 贵州盛达生植物发展有限公司 | A kind of cultural method of dendrobium candidum |
| CN111393224A (en) * | 2018-12-28 | 2020-07-10 | 中科实盈(广东)生物技术有限公司 | Method and apparatus for producing nano fertilizer and other types of solid and liquid organic-inorganic compound fertilizer and slow-release pesticide |
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Application publication date: 20110921 |
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