CN102178726B - Chinese medicinal composition and spraying agent for preventing emerging respiratory infectious diseases - Google Patents
Chinese medicinal composition and spraying agent for preventing emerging respiratory infectious diseases Download PDFInfo
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Abstract
The invention relates to a Chinese medicinal composition and spraying agent for preventing emerging respiratory infectious diseases, belonging to the technical field of Chinese medicinal preparations. In the invention, the Chinese medicinal composition comprises the following raw medicinal herbs in parts by weight: 9-12 parts of red halloysite, 9-24 parts of indigowoad root and 9-24 parts of astragalus root; and the spraying agent comprises an active constituent and a medicinal preservative, wherein the active constituents comprise: (1) a product obtained by subjecting the indigowoad root andthe astragalus root to boiling and alcohol precipitation and (2) a product obtained by subjecting the red halloysite to milling and refining. The preparation provided by the invention has the advantages of safety, quick results, stability, convenience for use and easiness in production and is beneficial to the medicine use of mass populations.
Description
Technical field
The invention belongs to the Chinese medicine preparation technical field, be specifically related to a kind of Chinese medicine composition and spray of preventing emerging respiratory infectious diseases.
Background technology
In recent years, the change of natural environment and climate and the evolution of microorganism and variation cause the new and burst infectious disease frequent occurrence in the whole world, and their Unpredictability, danger and terrified property have brought huge challenge for national health support work.Wherein the harm that causes of the eruption and prevalence of airborne emerging respiratory infectious diseases is especially serious, from SARS in 2003 (severe acute respiratory syndrome, severe acute respiratory syndrome) eruption and prevalence is in recent years bird flu and H 1 N 1 influenza A virus infection event, and China is faced with the danger of more and more severeer sudden emerging respiratory infectious diseases eruption and prevalence.
Respiratory infectious disease is often take antibacterial and virus as main pathogens, have the sickness rate height, popular wide, propagate rapidly characteristics.Vaccine is the active drug of preventing respiratory infectious disease, but the development of sudden emerging infectious disease from the clinical diagnosis to the vaccine often needs some cycles, can't satisfy in a short time people are badly in need of prevention during the burst disease transmission demand; In addition, the variation of respiratory infectious disease pathogen even if some known pathogen are caused a disease, has time vaccines also can't bring into play good efficacy rapidly, causes popular on a large scale.Our influenza virus of knowing for example, always in the worldwide great outburst of having carried out the coffin upon burial of quiet a period of time processus aboralis, historic spanish influenza, Asia influenza, Mao flu and Russian influenza are because existing vaccine is invalid, all caused worldwide being very popular, M ﹠ M is high.At present, there is no in the world any active drug that can prevent the respiratory infectious disease that multiple sudden emerging respiratory infectious diseases or sudden variation pathogen cause.
Motherland's medical science is to infectious disease, particularly to the understanding of respiratory infectious disease with prevent and treat with a long history.Before more than 2,000 years, the Han dynasty Zhang Zhongjing just has relevant discussion to go out side by side the side's of control medicine.Chinese medicine has the treatment characteristic of " strengthening vital QI to eliminate pathogenic factors " " treating the same disease with different methods, treating different diseases with the same therapeutic principle ", is particularly useful for Protean respiratory infectious disease.In recent years, preventing and treating in the process of the burst deadly infectious diseases such as SARS and influenza A, Chinese Traditional Medicine has become important " sharp sword " that China resists new and SARS Epidemic, has brought into play unique effect, is subject to the height approval of the Chinese government and World Health Organization (WHO).Because Chinese medicine has the characteristics such as interaction between multicomponent, many target spots, many effects and the composition, so that Chinese medicine can be by a plurality of link control respiratory infectious diseases.Studies show that many Chinese medicines have suppress virus replication, adsorb pathogen, stop pathological changes caused by virus, regulate immunologic function, improve pulmonary circulation, the comprehensive effect such as antalgic and inflammation relieving.As seen, Chinese medicine has unique advantage aspect the control respiratory infectious disease.
The Chinese patent medicine of the domestic control respiratory infectious disease commonly used of having developed mainly comprises " Radix Isatidis granules " " antivirus oral liquid ", " SHUANGHUANGLIAN KOUFUYE " etc. at present, and curative effect is clear and definite, but then has obvious deficiency as military medicine.The specific use object in wartime and special storing environment have proposed special requirement to military medicine, as safety, quick-acting, stable, conveniently use, be beneficial to large crowd's medication etc.Be to reduce non-combat casualty, the prevention of propagating respiratory infectious disease rapidly is particularly important.How to utilize the effect characteristics of Chinese medicine, learn achievement in research in conjunction with modern Chinese medicine, the too many levels of maximum possible prevents various known or even new RB road transmission diseases to become one of focus that people pay close attention to.
Purpose of the present invention is exactly a kind of safe, quick-acting, stable, spray of conveniently using, being easy to produce, be beneficial to large crowd's medication of research, also can equip our troops and satisfy specific use object and special storing environment in army's wartime, the dual-use army with multiple link preventing respiratory infectious disease and national new drug.
Summary of the invention
The objective of the invention is in order to solve emerging respiratory infectious diseases from occurring to before the vaccine development success, without the problem of preventive medicine effectively, disclose a kind of Chinese medicine composition for the treatment of emerging respiratory infectious diseases in a short time.
Another object of the present invention has been to disclose a kind of spray for the treatment of emerging respiratory infectious diseases.
Technical scheme of the present invention is as follows:
A kind of Chinese medicine composition of preventing emerging respiratory infectious diseases, wherein, described compositions comprises the crude drug of following weight parts: 9～12 parts of Halloysitum Rubrums, 9～24 parts of Radix Isatidis, 9～24 parts of the Radixs Astragali.
The Chinese medicine composition of the preventing emerging respiratory infectious diseases described in the technique scheme, wherein, the weight portion of described each crude drug is: 10～11 parts of Halloysitum Rubrums, 13～20 parts of Radix Isatidis, 13～20 parts of the Radixs Astragali.
The Chinese medicine composition of the preventing emerging respiratory infectious diseases described in the technique scheme, wherein, the ratio of the weight portion of described each crude drug is Halloysitum Rubrum: Radix Isatidis: the Radix Astragali equals 1: 1: 1.
A kind of spray of preventing emerging respiratory infectious diseases, described spray comprise active component and pharmaceutically acceptable carrier, and wherein, described active component is by the described Chinese medicine composition preparation of arbitrary technical scheme in the technique scheme.
The spray of the emerging respiratory infectious diseases described in the technique scheme, wherein, described active component comprises:
(1), Radix Isatidis and the Radix Astragali product behind water boiling and precipitation with ethanol; With
(2), the product of Halloysitum Rubrum after the refinement of milling.
The product of the Radix Isatidis in the technical program and Radix Astragali water boiling and precipitation with ethanol and Halloysitum Rubrum all satisfy spray to the requirement of granular size through the product after the micronizing on particle diameter; As the optimal technical scheme of the technical program, the product of Halloysitum Rubrum after micronizing crossed 325 mesh sieves.
The spray of the preventing emerging respiratory infectious diseases described in the technique scheme, wherein, pharmaceutically acceptable carrier is sodium carboxymethyl cellulose and sodium benzoate, wherein the quality of sodium carboxymethyl cellulose is 0.3% of spray herb liquid final volume, and the quality of sodium benzoate is 0.5% of spray herb liquid final volume.
The spray of the emerging respiratory infectious diseases described in the technique scheme, wherein, each material can evenly mix in the active component suspension in order to make, and active component adopts following method preparation in the technical program:
(1), takes by weighing Halloysitum Rubrum, Radix Isatidis and the Radix Astragali of described weight portion;
After Radix Isatidis and the Radix Astragali being soaked, decoct 3 times, merge decocting liquid (2), respectively, concentrated after the 70% ethanol precipitation;
(3), the spray active component is respectively by following (i) or (ii) two kinds of different paths preparations:
(i) Halloysitum Rubrum adds water retting, then adds in step (2) products therefrom, and after fully stirring, the refinement of milling is cooled to room temperature after stirring under the heating, makes suspension through fully stirring, and namely gets the active component of spray; Or
(ii) to the Halloysitum Rubrum refinement of milling, then add in step (2) products therefrom, fully stir, make suspension, namely get the active component of spray.As the further preferred version to the technical program, the grain diameter described in the path (i) of step (3) in the suspension is crossed 325 mesh sieves, described in path (ii) in the suspension particle diameter of Halloysitum Rubrum cross 325 mesh sieves; Preferred pharmaceutically acceptable carrier is sodium carboxymethyl cellulose and sodium benzoate, and wherein the quality of sodium carboxymethyl cellulose is 0.3% of spray herb liquid final volume, and the quality of sodium benzoate is 0.5% of spray herb liquid final volume.
The spray of the emerging respiratory infectious diseases described in the technique scheme, wherein, pharmaceutically acceptable carrier is sodium carboxymethyl cellulose and sodium benzoate, wherein the quality of sodium carboxymethyl cellulose is 0.3% of spray herb liquid final volume, and the quality of sodium benzoate is 0.5% of spray herb liquid final volume.
Chinese medicine composition of the present invention can adopt multiple dosage form, except the spray described in the technique scheme, Chinese medicine composition of the present invention also with pharmaceutically acceptable carrier combinations, make gel or nasal drop and be applied to the nasal membrane surface.
As the extension to the preparation method of the spray of a kind of preventing emerging respiratory infectious diseases of the present invention, another object of the present invention has been to disclose the preparation method of spray of the present invention.
Technical scheme is as follows:
A kind of preparation method of spray of preventing emerging respiratory infectious diseases comprises the steps:
(1), takes by weighing the crude drug of following weight parts: 9～12 parts of Halloysitum Rubrums, 9～24 parts of Radix Isatidis, 9～24 parts of the Radixs Astragali;
After Radix Isatidis and the Radix Astragali being soaked, decoct 3 times, merge decocting liquid (2), respectively, concentrated after the 70% ethanol precipitation;
(3), the spray active component is respectively by following (i) or (ii) two kinds of different paths preparations:
(i) Halloysitum Rubrum adds water retting, then adds in step (2) products therefrom, after fully stirring, after the refinement of milling, is cooled to room temperature after stirring under the heating, makes suspension through fully stirring, and namely gets the active component of spray; Or
(ii) to the Halloysitum Rubrum refinement of milling, then add in step (2) products therefrom, fully stir, make suspension, namely get the active component of spray;
(4), add pharmaceutically acceptable carrier in the active component in step (3), namely get the spray medicinal liquid of preventing respiratory infectious disease.
Preparation method described in the technique scheme, wherein, the weight portion of each crude drug is in the step (1): 10～11 parts of Halloysitum Rubrums, 13～20 parts of Radix Isatidis, 13～20 parts of the Radixs Astragali.
Preparation method described in the technique scheme, wherein, the ratio of the weight portion of each crude drug is Halloysitum Rubrum in the step (1): Radix Isatidis: the Radix Astragali equals 1: 1: 1.
Preparation method described in the technique scheme, wherein, the grain diameter described in the path (i) of step (3) in the suspension is crossed 325 mesh sieves, particle diameter 325 mesh sieves of Halloysitum Rubrum in the suspension described in path (ii).As the optimal technical scheme of the technical program, the pharmaceutically acceptable carrier described in the step (4) is sodium carboxymethyl cellulose and sodium benzoate; The quality of the sodium carboxymethyl cellulose described in the preferred step (4) is 0.3% of spray herb liquid final volume, and the quality of sodium benzoate is 0.5% of spray herb liquid final volume.
Preparation method described in the technique scheme, wherein, the pharmaceutically acceptable carrier described in the step (4) is sodium carboxymethyl cellulose and sodium benzoate; The quality of the sodium carboxymethyl cellulose described in the preferred step (4) is 0.3% of spray herb liquid final volume, and the quality of sodium benzoate is 0.5% of spray herb liquid final volume.
The above-mentioned thinning process of milling of stocking in the method for the present invention is to carry out in colloid mill.
In Chinese medicine composition of the present invention and follow-up preparation spray process, can also add Flos Lonicerae, Folium Isatidis etc. in the Chinese medicine composition of the present invention and have antiviral Chinese herb, have identical effect at preventing emerging respiratory infectious diseases.
The effect of each crude drug is as follows in the Chinese medicine composition of the present invention:
Halloysitum Rubrum is the minerals as Chinese medicine with the red blue spray crystal structure of octahedral among the present invention, the Halloysitum Rubrum micropowder is sprayed at oral cavity and nasal membrane surface, the toxin of pathogenic bacterium, virus and their generations can be adsorbed, fixes, excrete by nasal mucus, be similar to the respiratory tract entrance and added the invisible protective shielding of one deck, can directly effectively cut off the respiratory infectious disease route of transmission, reach the effect of direct effective preventing respiratory infectious disease.
Radix Isatidis for effective broad-spectrum antiseptic, anti virus herb, also has antiendotoxin and immunoregulation effect through Long-term Clinical Observation simultaneously among the present invention.Radix Isatidis effective site is sprayed at the respiratory tract entrance, can directly contacts pathogen and bring into play rapidly the effect that suppresses to kill pathogen; The Radix Isatidis effective substance is organized absorbed into serum by oral cavity and nasal membrane, can improve immunity of organisms and inhibition and kill the pathogen that enters individually in the blood, reaches the effect of more effective preventing respiratory infectious disease.
The Radix Astragali is famous tonification class Chinese medicine among the present invention, but short-term improves rapidly immunity of organisms.Radix Astragali effective site is sprayed at the respiratory tract entrance, can directly contacts pathogen and bring into play rapidly the effect that suppresses to kill pathogen; Radix Astragali effective substance is organized absorbed into serum by oral cavity and nasal membrane, and blocking-up influenza viroid absorption host cells infected also has inhibitory action to being adsorbed in cell surface and entering intracellular virus, reaches the effect of more effective preventing respiratory infectious disease.
Owing to adopted technique scheme, the present invention to have following beneficial effect:
1, the Halloysitum Rubrum in the Chinese medicine composition of the present invention is physical property absorption pathogen, Radix Isatidis is kill virus directly, the Radix Astragali can improve rapidly immunity of organisms, this protection is all effective to most of pathogen, remedied and faced clinically the emerging respiratory infectious diseases their early stage, the deficiency of the effective prophylactic agent of nothing before vaccine is not succeeded in developing.Simultaneously, this protective action can be not due to illness poison variation and losing efficacy, can not produce drug resistance yet, evaded the defective of most antiviral drugs and antibacterials, to preventing unpredictalbe emerging respiratory infectious diseases, can effectively subtract and cut off the route of transmission, protection Susceptible population.
2, the spray of the present invention Chinese patent medicine that overcome existing control respiratory infectious disease on the market uses, carries inconvenience, is not suitable with the specific use object in wartime and the shortcoming of special storing environment, and clinically without the medicine of similar effect characteristics.Adopt the medication administration method of high concentration spraying; can make medicine enter the interruption-forming guard shield by respiratory tract respectively and cut off the route of transmission, improve body self resistance against diseases and directly kill three kinds of modes of pathogen, bring into play the multipath preventing respiratory infectious disease effect of similar HAART.The army and the people's preventing respiratory infectious disease is used, evident in efficacy.
3, preparation safety of the present invention, quick-acting, stable, conveniently use, be easy to produce, be beneficial to large crowd's medication.
4, among the present invention with Halloysitum Rubrum or Halloysitum Rubrum with after Radix Isatidis and Radix Astragali extract mix, 325 mesh sieves are crossed in the refinement of milling, so that each composition can Uniform Dispersion in the prepared active component suspension.
The specific embodiment
For making technical scheme of the present invention be convenient to understand, the present invention is further illustrated below in conjunction with the specific embodiment
Embodiment 1:The preparation of spray active component
Take by weighing Halloysitum Rubrum 900g, Radix Isatidis 1200g, Radix Astragali 1200g; After Radix Isatidis and the Radix Astragali added 8 times of water soakings, decocting 30min filtered after the 70% ethanol precipitation respectively, revolves the inspissation contracting; Halloysitum Rubrum adds 5 times of water rettings, then adds the extract of Radix Isatidis and the Radix Astragali, fully stirs, and aforesaid liquid is put into that colloid mill mills after the refinement can mistake 325 mesh sieves, namely get the active component of spray.
Embodiment 2:The preparation of spray active component
Take by weighing Halloysitum Rubrum 1000g, Radix Isatidis 1500g, Radix Astragali 1500g; After Radix Isatidis and the Radix Astragali added 10 times of water soakings, decocting 20min filtered after the 70% ethanol precipitation respectively, revolves the inspissation contracting; Halloysitum Rubrum adds 5 times of water rettings, then adds the extract of Radix Isatidis and the Radix Astragali, fully stirs, and aforesaid liquid is put into that colloid mill mills after the refinement can mistake 300 mesh sieves, namely get the active component of spray.
Embodiment 3:The preparation of spray active component
Take by weighing Halloysitum Rubrum 1100g, Radix Isatidis 2000g, Radix Astragali 2000g; After Radix Isatidis and the Radix Astragali added 10 times of water soakings, decocting 30min filtered after the 70% ethanol precipitation respectively, revolves the inspissation contracting; Halloysitum Rubrum adds 3 times of water rettings, then adds the extract of Radix Isatidis and the Radix Astragali, fully stirs, and aforesaid liquid is put into that colloid mill mills after the refinement can mistake 400 mesh sieves, namely get the active component of spray.
Embodiment 4:The preparation of spray active component
Take by weighing Halloysitum Rubrum 1200g, Radix Isatidis 2400g, Radix Astragali 2400g; After Radix Isatidis and the Radix Astragali added 20 times of water soakings, decocting 20min filtered after the 70% ethanol precipitation respectively, revolves the inspissation contracting; Halloysitum Rubrum adds 3 times of water rettings, then adds the extract of Radix Isatidis and the Radix Astragali, fully stirs, and aforesaid liquid is put into that colloid mill mills after the refinement can mistake 325 mesh sieves, namely get the active component of spray.
Test example 5:The preparation of spray medicinal liquid
The spray active component of embodiment 1～4 preparation gained is cooled to room temperature after stirring under the heating, add purified water to 1000ml, add 3g sodium carboxymethyl cellulose and 5g sodium benzoate, make suspension through fully stirring, namely get spray medicinal liquid of the present invention.
Embodiment 6:The preparation of spray
The spray medicinal liquid adding of embodiment 5 preparations is expected namely to get spray of the present invention in the bottle with the medicinal of quantitative atomizing pump.
Below specifically test by the pharmacodynamics of spray of the present invention, the beneficial effect that the present invention has is described:
Influenza virus A type, the influenza virus that relates in the following test example is B-mode, respiratory syncytial virus strain, MDCK, Hep-2 cell strain, MA104 cell strain, Flu-A and RSV strain are market and buy, and the RPMI-1640 culture fluid is available from Merk company; MTT is available from Sigma company, and kunming mice is available from animal institute of Military Medical Science Institute, and spray of the present invention is for to prepare gained according to embodiment 1 scheme.
Test example 1: spray antivirus action of the present invention
1.1 external inactivation of virus test
Mdck cell and Hep-2 cell are inoculated respectively in influenza virus A type, influenza virus that lyophilizing is preserved is B-mode, respiratory syncytial virus strain, put under 37 ℃, 5%CO2 condition with cell maintenance medium and to cultivate, Hep-2, mdck cell digestion in the Tissue Culture Flask are disperseed, respectively with 10
5/ ml cell concentration is inoculated 96 orifice plates, 5%CO
2, 37 ℃ of cultivations, grow up to monolayer behind the 2d, for subsequent use.Get the spray of the present invention (1mg, 2mg, 4mg) of various dose, (48h TCID50 is 10 to add herpes simplex virus
-6.0), (120h TCID50 is 10 to influenza virus
-3.5), (48h TCID50 is 10 to RSV
-5.0) mix, put 1h for 37 ℃, use afterwards the centrifugal 10min of 2000r/min, supernatant is done 10 multiple proportions serial dilutions, be inoculated in successively (herpes simplex virus and respiratory syncytial virus inoculation Heo-2 on the corresponding cell in 96 orifice plates, influenza virus inoculation MDCK), each dilution factor vertically repeats 3 holes, 37 ℃, 5%CO
2Cultivate, influenza virus is cultivated and stops cultivating after 120h, herpes simplex virus and respiratory syncytial virus are cultivated 48h, and neutral red staining reads the A540 value, calculates the pathological changes suppression ratio, obtains TI.
The result shows, the spray of the present invention of variable concentrations (100ug/ml, 200ug/ml, 400ug/ml) infected by influenza first type, influenza virus are B-mode, respiratory syncytial virus all has good deactivation, through at 37 ℃, 5%CO
2Constant incubator is hatched 60min, flu-A TCID50 1 index that descends respectively, flu-B 3 indexes that descend, RSV 5 indexes that descend.Show that the spray of the present invention of 100ug/ml, 200ug/ml, 400ug/ml variable concentrations all has the effect of powerful viral adsorption.
Table 1 spray of the present invention is on the impact of viral infection virulence TCID50
Annotate: in the bracket be and the more viral TCID50 decline of virus control group index
1.2 vitro Adsorption test
Add first the medicinal liquid that series is used RPMI-1640 culture fluid doubling dilution in 96 well culture plates of the MA104 cell that grows into monolayer, the maximum concentration of medicinal liquid is medicine maximal non-toxic concentration, and each liquor strength repeats 4 holes, 100 μ L/ holes.If normal cell matched group, virus control group all only add isometric(al) RPMI-1640 culture fluid.37 ℃ of 5%CO2 are hatched 2h, with the medicinal liquid sucking-off, add virus (37 ℃ of effects of the pancreatin of virus and 10 μ g/ml concentration 30min) the 100 μ L/ holes of 100TCID50 except Normal group.37 ℃ of 5%CO2 are hatched 2h, with viral sucking-off, add cell maintenance medium 200 μ L/ holes, and 37 ℃ of 5%CO2 are hatched, and Continuous Observation.Treat that cell CPE occurs and exists ++～+++time is the maintenance medium sucking-off, adds the MTT 50 μ L/ holes of 5mg/ml, abandons supernatant after hatching 2h.Add DMSO 50 μ L/ holes, the about 10min of concussion mixing under the room temperature.Enzyme-linked immunosorbent assay instrument reads absorbance under wavelength 570nm subsequently.Calculate medicine to the suppression ratio (the results are shown in Table 2) of virus according to following formula.The spray infected by influenza first type of the present invention of 25ug/ml, 50ug/ml, 100ug/ml, 200ug/ml and 400ug/ml, influenza virus are B-mode, respiratory syncytial virus all has good adsorption, and the dosage adsorption rate of 100ug/ml is all more than 50%.
Table 2 spray of the present invention is at the adsorption (n=4) of MA104 cell to first type stream pipe virus, Influenza B virus, respiratory syncytial virus
Compare with the blank group,
##P＜0.01; Compare with the virus group,
*P＜0.01
1.3 in vivo test
Kunming kind Mus, body weight 13～14g.Divide at random the large, medium and small dosage treatment group of Normal group, virus model matched group, ribavirin matched group and spray of the present invention and prevention group, 10 every group with animal by body weight.Prevention group 1h before virus inoculation gives and spray collunarium of the present invention.Under etherization, normal group animal intranasal inoculation cell culture fluid 20 μ l, the equal intranasal inoculation respiratory syncytial virus of all the other animals or influenza A virus venom 20 μ l, spray of the present invention is given and the collunarium intervention according to 100ug/ml, 200ug/ml, 400ug/ml, every day 2 times, collunarium is 2 continuously.Observe the animal nasal cavity every day whether whether secretions, animal scratch nasal cavity phenomenon, survival condition are arranged, behind 5d last administration 2h, the animal rear ophthalmic artery clump of weighing is got blood, leads the neck mortar and put to death, get lung tissue under the aseptic condition, do lung tissue virus content detection.
(1) respiratory syncytial virus assay: will inoculate each treated animal lung tissue glass homogenizer homogenate of RSV, the cell maintenance medium piping and druming that adds 1000 μ l suspends, the centrifugal 10mim of 10000r/min, laterally add successively on the MA104 cell that has grown up to monolayer in 96 orifice bores after doing again continuous 2 multiple proportions serial dilutions (2-1,2-2,2-3,2-4), vertically repeat 3 holes.Be provided with simultaneously blank (acellular growth).37 ℃, 5%CO2 cultivation, every day, cytopathy person appearred in the observation of cell pathological changes, was judged to virus and separated positive.The 5d record is last toxic concentration eventually, the statistics virus isolated rate, and relatively group difference is judged the effect that spray treatment upper respiratory tract infection of the present invention is prevented lower respiratory infection.
(2) influenza A virus assay: will inoculate each treated animal lung tissue glass homogenizer homogenate of Flu-A, the cell maintenance medium piping and druming that adds 1000 μ l suspends, the centrifugal 10mim of 10000r/min, laterally add in the blood-coagulation-board with continuous 2 multiple proportions serial dilutions (2-1,2-2,2-3,2-4), vertically repeat 3 holes, then adding waits the Sanguis Gallus domesticus ball (0.2%) of capacity, record blood clotting situation during room temperature 40min.
The result shows: the virus group of inoculation RSV has 8 zoogenetic infections, positive drug matched group to have 6 infection, the large, medium and small dosage group of spray of the present invention all not to be separated to virus; The blood clotting positive appears in 8 parts of inoculation Flu-A virus groups, and the blood clotting positive appears in 7 parts of positive drug matched groups, and the large, medium and small dosage group of spray of the present invention respectively has 1 part the blood clotting positive to occur.Show that spray of the present invention can effectively stop Respirovirus to develop to lower respiratory tract, control effectively.
The above, it only is preferred embodiment of the present invention, be not that the present invention is done any formal and substantial restriction, all those skilled in the art, within not breaking away from the technical solution of the present invention scope, when can utilizing the disclosed above technology contents, and a little change of making, modify the equivalent variations with differentiation, be equivalent embodiment of the present invention; Simultaneously, the change of any equivalent variations that all foundations essence technology of the present invention is done above embodiment, modify and differentiation, all still belong in the scope of technical scheme of the present invention.
Claims (8)
1. the Chinese medicine composition of a preventing emerging respiratory infectious diseases is characterized in that, described compositions is made by the crude drug of following weight parts: 9～12 parts of Halloysitum Rubrums, 9～24 parts of Radix Isatidis, 9～24 parts of the Radixs Astragali.
2. the Chinese medicine composition of preventing emerging respiratory infectious diseases according to claim 1 is characterized in that, the weight portion of described each crude drug is: 10～11 parts of Halloysitum Rubrums, 13～20 parts of Radix Isatidis, 13～20 parts of the Radixs Astragali.
3. the Chinese medicine composition of preventing emerging respiratory infectious diseases according to claim 1 is characterized in that, the ratio of the weight portion of described each crude drug is Halloysitum Rubrum: Radix Isatidis: the Radix Astragali equals 1:1:1.
4. the spray of a preventing emerging respiratory infectious diseases, described spray comprises active component and pharmaceutically acceptable carrier, it is characterized in that: described active component is by the described Chinese medicine composition preparation of arbitrary claim in the claim 1～3.
5. the spray of emerging respiratory infectious diseases according to claim 4, it is characterized in that: described active component comprises:
(1), Radix Isatidis and the Radix Astragali product behind water boiling and precipitation with ethanol; With
(2), the product of Halloysitum Rubrum after the refinement of milling.
6. according to claim 4 or the spray of 5 described preventing emerging respiratory infectious diseases, it is characterized in that: pharmaceutically acceptable carrier is sodium carboxymethyl cellulose and sodium benzoate, wherein the quality of sodium carboxymethyl cellulose is 0.3% of spray herb liquid final volume, and the quality of sodium benzoate is 0.5% of spray herb liquid final volume.
7. according to claim 4 or the spray of 5 described preventing emerging respiratory infectious diseases, it is characterized in that described active component is by following method preparation:
(1), takes by weighing Halloysitum Rubrum, Radix Isatidis and the Radix Astragali of described weight portion;
After Radix Isatidis and the Radix Astragali being soaked, decoct 3 times, merge decocting liquid (2), respectively, concentrated after the 70% ethanol precipitation;
(3), the spray active component is respectively by following (i) or (ⅱ) two kinds of different paths preparations:
(i) Halloysitum Rubrum adds water retting, then adds in step (2) products therefrom, and after fully stirring, the refinement of milling is cooled to room temperature after stirring under the heating, makes suspension through fully stirring, and namely gets the active component of spray; Or
(ⅱ) to the Halloysitum Rubrum refinement of milling, then add in step (2) products therefrom, fully stir, make suspension, namely get the active component of spray.
8. the spray of preventing emerging respiratory infectious diseases according to claim 7, it is characterized in that: the grain diameter described in the path (i) of step (3) in the suspension is crossed 325 mesh sieves, described in path (ⅱ) in the suspension particle diameter of Halloysitum Rubrum cross 325 mesh sieves.
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