CN102178233A - Spirulina nutrient solution - Google Patents
Spirulina nutrient solution Download PDFInfo
- Publication number
- CN102178233A CN102178233A CN2011101021548A CN201110102154A CN102178233A CN 102178233 A CN102178233 A CN 102178233A CN 2011101021548 A CN2011101021548 A CN 2011101021548A CN 201110102154 A CN201110102154 A CN 201110102154A CN 102178233 A CN102178233 A CN 102178233A
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- China
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- spirulina
- nutrient solution
- algae liquid
- solution
- papain
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Abstract
The invention provides a spirulina nutrient solution, which is prepared by the following steps of mixing spirulina powder and water into a spirulina solution, adding the papain into the spirulina solution, carrying out enzymolysis at a temperature of 60 DEG C to 68 DEG C, and carrying out high-speed centrifugal filtration to obtain a clear and transparent spirulina nutrient solution. The spirulina nutrient solution can greatly enhance the direct utilization ratio of the spirulina protein, and can ensure that the spirulina nutrients including amino acid, vitamin B1, vitamin B2, Beta-carotene, blue-green algae, calcium, Fe, Zn, Mg and K are completely dissolved in the solution. Compared with solid spirulina tablets, capsules and powder, the spirulina nutrient solution is more easy to absorb and utilize by human bodies, so the spirulina nutrient solution can meet the nutrition requirements of the aged, infants, patients and most people and has better and faster positive effects on human body health care function. The spirulina nutrient solution of the invention can be prepared into various kinds of beverages or drinks, which is convenient for people of different ages and different physical conditions to drink.
Description
Technical field
The present invention relates to the preparation method of a kind of food, health products, especially a kind of nutritious algae liquid and preparation method thereof.
Background technology
Spirulina solid food (tablet, capsule, pulvis) certain scale and influence have all been arranged in recent years, but liquid spirulina (nutritious algae liquid) does not have also in current market at aspects such as manufacturing and marketing and consumer benefit from.Though have the people to deliver document in theory, producing and technical not practicality.Someone proposes only need add protease or compound protease 2 hours in theory, protein transduction that just can the enzymolysis spirulina powder turns to several amino acids, operation is impracticable fully technically, the algae liquid of 2 hours enzymolysis is green, and can not present the flaxen true qualities of amino acid, so its reality does not also turn to amino acid to protein transduction.The preparation method of the nutritious algae liquid that someone proposes, general owing to it, just notion does not have the practical operation meaning, can't be made as the value of edible nutritious algae liquid, so market is still blank so far.
Summary of the invention
The objective of the invention is to develop and a kind ofly change into the method for liquid spirulina nutrient solution, improving the direct utilization rate of hydrolysis of protein spirulina, thereby satisfy most people nutritional need of (comprising the infant) from the solid spirulina.
The technical solution used in the present invention is:
The present invention is with papain (Latin: chaenomeles lagenaria koidz, hereinafter to be referred as papain) at high temperature (60-68 ℃) after down the enzymolysis spirulina powder adds the algae liquid that water stirs into, make most protein transduction of spirulina powder change into the several amino acids solution of spirulina, after high speed centrifugation filters, draw limpid bright algae liquid, make can be edible nutritious algae liquid.
The preparation method is:
Spirulina powder+water is stirred into algae liquid, add papain and under 60-68 ℃ of temperature, carry out enzymolysis, filter through high speed centrifugation again, obtain limpid bright nutritious algae liquid.
The algae liquid that described spirulina powder+water stirs into is meant in 100-150 and rises the ratio that drinking water drops into 20 kilograms of spirulina powders.
The papain addition rises algae liquid by 100-150 and adds 2.0-2.5 kilogram papain, and described papain is 800,000-1,000,000 u/g (ten thousand units).
Nutritious algae liquid of the present invention can be mixed with various beverages or drink, makes things convenient for the people of all ages and classes, different physique to enjoy.
Advantage of the present invention and good effect:
The present invention can improve the direct utilization rate of hydrolysis of protein spirulina greatly.Because spirulina tablet, capsule, pulvis, human body must just can absorb by protein transduction being changed into amino acid, the existing method that changes into liquid spirulina from the solid spirulina, do not carry out breaking-wall cell, absorb so human body is difficult, and method of the present invention has been carried out broken wall to the cell of spirulina, nutritious algae liquid changes into several amino acids with containing a large amount of (more than 70%) protein transductions in the spirulina, can directly form new human body protein for human body, thereby can satisfy most people nutritional need of (comprising old man, infant and patient).
The present invention can make the nutrition of spirulina: amino acid, Cobastab
1, B
2, beta carotene, cyanophycin, calcium, iron, zinc, magnesium, potassium all be dissolved in the solution, than solid spiral algae sheet, capsule, the pulvis human body is easier absorbs.Performance is to the good effect better faster of human body heath care function.Just eat solids such as spiral algae sheet, pulvis, capsule and absorb bad people for some, can easier absorption by edible nutritious algae liquid, utilize its balanced nutrition, especially old man, child, seriously ill person are more played active and effective health-care efficacy.
The specific embodiment
Weighing high-quality spirulina powder (meets " winkle spirulina powder " standard, be that " winkle spirulina powder " standard that standard GB/issue of T16919-1997 State Bureau of Technical Supervision is implemented is drawn materials), the ratio that rises 20 kilograms of drinking water adding spirulina powders according to 100-150 feeds intake.The papain of weighing 800,000-1,000,000 u/g again, promptly be in the 100-150 premium on currency with the ratio of the 2.0-2.5 kilogram papain that feeds intake, stir algae powder and papain then it thoroughly be dissolved in the water.Become dilution spirulina powder papain mixed liquor.
Energising heats up then, just makes it begin to take place enzymolysis.
The condition that should control during the beginning enzymolysis maintains the temperature between 60-68 ℃, is sure not too high.
Played the agitator stirring once, each 5-10 minute in every 1-2 hour during enzymolysis.
Continue enzymolysis time at 10-12 hour.
PH value in the high temperature enzymolysis solution is 6.5, and this moment, reaction speed was best.
The mistake of enzyme molten (enzyme goes out).After enzymolysis finishes, need heating enzymolysis liquid to 85 ℃, be incubated 5-10 minute, as the enzyme that goes out, the end enzymolysis.
Cooling: behind the enzyme that goes out (after high temperature 85-90 ℃ 5-10 minute), be cooled to below 40 ℃ with the warm water circulation rapidly, just enter filter process.
Filter: cooled enzymolysis algae liquid is introduced into sedimentation centrifuge just filters, liquid phase and solid are separated, take out solid phase algae mud, draw algae liquid.
The algae liquid that just leaches gets limpid bright smart filtrate through the smart filter of tubular-bowl centrifuge, and this is real nutritious algae liquid.
Embodiment 1
Get 10 kilograms of spirulina powders that meet GB, 50 liters of drinking water.Mix stirring evenly, put in the stainless steel enzymatic vessel after colloid mill twice gets spirulina magma.
Add 1.25 kilograms of papains (80u/g), startup is heated to 60-68 Celsius ℃ after stirring, and keeps temperature, makes it to carry out enzymolysis.Stirred 10 minutes every 1 hour.
Continue enzymolysis 10 hours, running check also keeps about pH6.5.Make it to enter best hydrolysis temperature, allow its lasting enzyme digestion reaction.
Naked-eye observation spirulina slurry is constantly thinning, dark green thin out gradually, becomes yellow slightly, and during enzyme digestion reaction carried out gradually, through 10-12 hour, enzymolysis liquid became yellowish-brown, and enzymolysis soon finishes.
Enzymolysis finishes, and the heating enzymolysis liquid kept 5 minutes between 85-90 ℃, as the enzyme that goes out, and the end enzymolysis.
Cooling is cooled to rapidly below 40 ℃, enters filter process.
Cooled enzymolysis algae liquid is introduced into sedimentation centrifuge just filters, liquid phase and solid are separated, take out solid phase algae mud, draw algae liquid.
The algae liquid that just leaches through the smart filter of tubular-bowl centrifuge, gets limpid bright smart filtrate again, and this is real nutritious algae liquid.
Embodiment 2
Get 20 kilograms of spirulina powders that meet GB, 150 liters of drinking water.Mix stirring evenly, put in the stainless steel enzymatic vessel after colloid mill twice gets spirulina magma.
Add 2.5 kilograms of papains (1,000,000 u/g), startup is heated to 60-68 Celsius ℃ after stirring, and keeps temperature, makes it to carry out enzymolysis.Stirred 15 minutes every 2 hours.
Continue enzymolysis 12 hours, running check also keeps about pH6.5.Make it to enter best hydrolysis temperature, allow its lasting enzyme digestion reaction.
Naked-eye observation spirulina slurry is constantly thinning, dark green thin out gradually, becomes yellow slightly, and during enzyme digestion reaction carried out gradually, through 12 hours, enzymolysis liquid became yellowish-brown, and enzymolysis soon finishes.
Enzymolysis finishes, and the heating enzymolysis liquid kept 10 minutes between 85-90 ℃, as the enzyme that goes out, and the end enzymolysis.
Cooling is cooled to rapidly below 40 ℃, enters filter process.
Cooled enzymolysis algae liquid is introduced into sedimentation centrifuge just filters, liquid phase and solid are separated, take out solid phase algae mud, draw algae liquid.
The algae liquid that just leaches through the smart filter of tubular-bowl centrifuge, gets limpid bright smart filtrate again, and this is a nutritious algae liquid.
Filter out seminal fluid, by relevant researchonthe technology batching, can goes out per 1 liter of spirulina seminal fluid and adds the high quality white granulated sugar of 200 grams then with vial or stainless steel cask is freezing or convert 1 times of can of pure water at once, divide pack into carton or plastic bottle flexible package or vial inner wrapping again, get the nutritious algae liquid finished beverage.
Also nutritious algae liquid can be added the drink that an amount of can of pure water ammonification match honey that dissolves 1 times becomes the 200-250 milliliter, drink for fearing sugared consumer (diabetes patient).
Claims (3)
1. a nutritious algae liquid is characterized in that: spirulina powder+water is stirred into algae liquid, add papain and carry out enzymolysis under 60-68 ℃ of temperature, filter through high speed centrifugation, obtain limpid bright nutritious algae liquid.
2. nutritious algae liquid according to claim 1 is characterized in that:
The algae liquid that described spirulina powder+water stirs into is meant in 100-150 and rises the ratio that drinking water drops into 20 kilograms of spirulina powders.
3. nutritious algae liquid according to claim 1 is characterized in that:
The papain addition rises algae liquid by 100-150 and adds 2.0-2.5 kilogram papain, and described papain is 800,000-1,000,000 u/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101021548A CN102178233A (en) | 2011-04-22 | 2011-04-22 | Spirulina nutrient solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101021548A CN102178233A (en) | 2011-04-22 | 2011-04-22 | Spirulina nutrient solution |
Publications (1)
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|---|---|
| CN102178233A true CN102178233A (en) | 2011-09-14 |
Family
ID=44564649
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| CN2011101021548A Pending CN102178233A (en) | 2011-04-22 | 2011-04-22 | Spirulina nutrient solution |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103013726A (en) * | 2012-12-12 | 2013-04-03 | 赵波 | Spirulina blue polysaccharide green bear, liquor and beverage and preparation method |
| CN109865131A (en) * | 2019-04-01 | 2019-06-11 | 海南林恒制药股份有限公司 | A kind of preparation of spirulina tablet and its preparation method and application improving cardio and vascular function |
| WO2020172850A1 (en) * | 2019-02-28 | 2020-09-03 | 马诗歌瑞生物科技江苏有限公司 | Protein nutrient solution produced from spirulina and preparation method therefor |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101768209A (en) * | 2009-01-05 | 2010-07-07 | 北京林业大学 | Hypotensive peptide with high in-vivo activity and preparation and purification method thereof |
-
2011
- 2011-04-22 CN CN2011101021548A patent/CN102178233A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101768209A (en) * | 2009-01-05 | 2010-07-07 | 北京林业大学 | Hypotensive peptide with high in-vivo activity and preparation and purification method thereof |
Non-Patent Citations (3)
| Title |
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| 《广州食品工业科技》 19990930 吴少雄等 酶法制取螺旋藻水解物的研究 9－11 1－3 第15卷, 第3期 * |
| 《食品科学》 19981231 徐建祥等 螺旋藻营养饮料的研制 第42至43页 1－3 第19卷, 第9期 * |
| 《食品科学》 20041231 钟耀广等 木瓜蛋白酶水解螺旋藻蛋白的研究 135－136 1－3 第25卷, 第9期 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103013726A (en) * | 2012-12-12 | 2013-04-03 | 赵波 | Spirulina blue polysaccharide green bear, liquor and beverage and preparation method |
| CN103013726B (en) * | 2012-12-12 | 2017-07-11 | 赵波 | The algae indigo plant green beer of polysaccharide and drinks and beverage and preparation method thereof |
| WO2020172850A1 (en) * | 2019-02-28 | 2020-09-03 | 马诗歌瑞生物科技江苏有限公司 | Protein nutrient solution produced from spirulina and preparation method therefor |
| CN109865131A (en) * | 2019-04-01 | 2019-06-11 | 海南林恒制药股份有限公司 | A kind of preparation of spirulina tablet and its preparation method and application improving cardio and vascular function |
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Application publication date: 20110914 |