CN102172118A - Method for identifying quality of cotton salt-tolerant seeds - Google Patents
Method for identifying quality of cotton salt-tolerant seeds Download PDFInfo
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- CN102172118A CN102172118A CN2011100411518A CN201110041151A CN102172118A CN 102172118 A CN102172118 A CN 102172118A CN 2011100411518 A CN2011100411518 A CN 2011100411518A CN 201110041151 A CN201110041151 A CN 201110041151A CN 102172118 A CN102172118 A CN 102172118A
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Abstract
The invention discloses a method for identifying quality of cotton salt-tolerant seeds. Salt-tolerant seeds are screened by adopting an indoor experiment and an outdoor experiment, wherein the indoor experiment comprises an indoor germination experiment I and/or an indoor germination experiment II; and the outdoor experiment comprises outdoor salt-tolerance identification I and/or outdoor salt-tolerance identification II. In the invention, the salt-tolerance character of cotton is integrally identified and evaluated by adopting double-layered filter papers, sand, salt ponds and heavy alkali-salinity soils by the seaside. The salt-tolerance in the germination stage, the seedling stage and the plant-forming stage of cotton can be identified and evaluated. The salt-tolerance of cotton is detected roundly and stably with high efficiency and high throughout. The integral identification and evaluation of multi-stage, multi-place and multi-level can be performed for the salt-tolerance of crops which are controlled by complicated quantitative inheritance genes and greatly influenced by external environment.
Description
Technical field
The present invention relates to agricultural technology field, especially the salt-enduring species matter authentication method that grows cotton.
Background technology
The whole world has 20% arable land salination at least, and the salinity of soil middle and high concentration causes osmotic stress and ion to poison to crop, thereby influences the growth of crop, seriously causes crop failure.China has salinization of soil and secondary salinization of soil soil 4,000 ten thousand hm approximately
2More than, become the key constraints that China's agricultural production develops.Jiangsu coast has wide saline land, and only Yancheng just has beach area 45.3 ten thousand hm
2More than, account for 70% of the whole province, and every year is also with 666.7hm
2Above speed is constantly outwards become silted up long.Jiangsu Province proposes " scientific and technological Xinghai County strategy " very early, and especially 2009 " jiangsu coast great development+Yangtze River Delta great development " rises to national strategy, and exploitation brings new opportunity to jiangsu coast.Screening and plant salt tolerant crop and carry out soil melioration, beach utilization, increasing peasant income huge meaning is arranged.And biodiesel, biomass power generation raw material, salt tolerant pioneer crop-cotton are ideal materials.Soil salt content is emerged, is grown being suitable for cotton below 0.2%, but soil salt concentration will produce harm to cotton greater than 0.3% o'clock.Therefore the saline land saliferous of jiangsu coast sheet presses for the salt resistance ability of cotton is brought up to 0.3-0.35% even higher level about 0.3%.So it is significant to estimate, excavate the germplasm resource for cotton that is fit to Jiangsu saline-alkali soil plantation, utilize marginal soil plantation living beings salt tolerant pioneer crops (cotton) such as coastal beach to tally with the national condition, alleviate the grain and cotton land conflict problem.The salt tolerance of crop shape is subjected to complex quantitative gene control, and it is very big to be affected by the external environment, and need carry out a plurality of periods, a plurality of place, a plurality of level integral body to the cotton salt-tolerance character and identify, estimates.
Summary of the invention
Technical problem to be solved by this invention is at the deficiencies in the prior art, and the salt-enduring species matter authentication method that grows cotton is provided.
The one salt-enduring species matter authentication method that grows cotton adopts laboratory experiment and outdoor experiment in conjunction with carrying out the screening of salt-enduring species matter, and described laboratory experiment comprises indoor germination experiment I and/or indoor germination experiment II; Described outdoor experiment comprises outdoor salt tolerant evaluation I and/or outdoor salt tolerant evaluation II.
Described method, described indoor germination experiment I is double-deck filter paper method, way is: below the plastics grid, the seed that will soak 0.4% salt solution again is placed on the filter paper with a filter paper pads of having soaked 0.4% salt solution, and then covers the filter paper that one deck soaked 0.4% salt solution.
Described method, described indoor germination experiment II adopts " Sha Peifa " to identify, described " Sha Peifa " adopts the square plastic container to pack into after fine sand germinates earlier by test material, the salt that claims fine sand weight 0.4% again, the water of fine sand weight 23%, stir evenly the back and import in the container, culture environment is the artificial culture case, and temperature is 30 degree Celsius.
Described method, described outdoor salt tolerant identify that I is the salt pond method: will salt resistance be reached the above material of anti-level proceed the salt pond evaluation through after indoor germination experiment I and/or the indoor germination experiment II.
Described method, described outdoor salt tolerant identify that II is a seashore geavy salt alkaline earth method, will identify that experiment material that I is accredited as the salt tolerant germplasm is planted in seashore geavy salt alkaline earth through indoor germination experiment I and/or indoor germination experiment II, outdoor salt tolerant and finally screen.
The present invention adopts double-deck filter paper, sand, salt pond, seashore geavy salt alkaline earth that cotton is carried out salt-tolerance character to carry out integral body and identify and evaluation method.Can identify, estimate cotton germination phase, seedling stage, one-tenth strain phase salt resistance.Comprehensively, stable, efficient, high flux detects the cotton material salt resistance.Can carry out the integral body of a plurality of periods, a plurality of place, a plurality of levels identifies, estimates for the salt tolerance of crop shape that is subjected to the control of complex quantitative gene and be affected by the external environment very big.
Description of drawings
Fig. 1 is the method flow diagram of embodiment 1.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1
(1) the double-deck filter paper of indoor germination experiment I-is identified
Double-deck filter paper method specific practice be earlier with a filter paper pads of having soaked salt solution (0.4% sodium chloride solution) below the plastics grid, the seed that will soak salt solution (0.4%) again is placed on the filter paper, and then covering one deck filter paper, top filter paper also soaks 0.4% salt solution.Test material comprises the salt tolerant strain: salt 1032, salt 1129, salt 2018. contrasts: cotton No. 9 of Soviet Union, anti-No. 1 of nasal mucus (other non-symptom of a trend materials slightly).Each material 1 row, 16 of every row are set up two repetitions, are divided into salt and handle and blank (table 2).Upper strata filter paper need be taken off every day and soak salt solution (0.4%).Culture environment is artificial incubator, and temperature is made as 30 degree.Germinateed, and added up germination rate after six days in three days.
Table 25 test material
(as table 3: salt 1032 reaches high anti-, and salt 1129 reaches anti-salt, and salt 2018 reaches salt tolerant as can be seen from the results; Salt 1032, salt 1129, salt 2018, anti-No. 1 of nasal mucus, cotton No. 9 salt resistance abilities of Soviet Union reduce successively; Salt is handled and the blank significant difference, and salt 1032 does not have difference substantially between two processing, but the length of germinateing is variant; Basically identical as a result between two repetitions;
The chitting piece number of table 3 different materials
(2) the husky training of indoor germination experiment II-
After carrying out indoor germination experiment I or with indoor germination, test simultaneously, adopt " Sha Peifa " to identify, can certainly adopt " Sha Peifa " to identify separately and do not carry out indoor germination experiment I (be indoor germination experiment I and indoor germination experiment II can adopt one separately or adopt simultaneously); " Sha Peifa " adopts the square plastic container to pack fine sand into by test material elder generation germination back (the back salt stress of emerging, if earlier with salt can be smelly, become tight, airtight, be difficult for germinateing), the salt that claims fine sand weight 0.4% again, the water of fine sand weight 23%, stirring evenly the back imports in the container, culture environment is the artificial culture case, and temperature is 30 degree Celsius.Statistics after two weeks, material of every row, 10.Test material comprises the salt tolerant strain: salt 1032, salt 1129, salt 2018. contrasts: anti-No. 1 of cotton No. 9 nasal mucus of Soviet Union (other non-symptom of a trend materials slightly).Fine sand heavily is 1476.13g among the present invention, adds pure water 295.2g, sodium chloride 5.9g.The result: salt 1032, salt 1129, salt 2018, anti-No. 1 of nasal mucus, cotton No. 9 salt resistance abilities of Soviet Union reduce successively.Result and method (1) are identical substantially, can identify Seedling Salt-tolerance.
(3) outdoor salt tolerant is identified the I-salt pond
After indoor germination experiment I of process and/or the indoor germination experiment II, salt resistance is reached the above material of anti-level (＞50%) proceed the salt pond evaluation, the salt pond is identified and is further screened in seedling stage.
Carried out the salt pond in 2006 and identify (0-0.5%), experimental scheme adopts two factor completely randomized experiments (6 of test materials, 5 of salinity levels, totally 30 processing), test material is the salt tolerant strain: salt 1032, salt 1129, salt 2018. contrasts: cotton No. 9 of Soviet Union, anti-No. 1 of nasal mucus, cotton 22 (other non-symptom of a trend materials slightly) of Soviet Union.5 of salinity levels: CK 0%, 0.20%, 0.30%, 0.40%, 0.50%.Test index has dead leaf rate of emergence rate, relative growth in seedling stage amount, seedling stage etc.Main Conclusions is: the salt tolerant (salt 1032) of material 5, material 3 (salt 1129) salt resistance secondly, material 4 is salt tolerant (cotton No. 9 of Soviet Union) least, suitable salinity 0-0.2%, optimal selection concentration 0.3%-0.4%.
(4) outdoor salt tolerant is identified II-seashore geavy salt alkaline earth
1. geavy salt alkaline earth selection of salt tolerance
Outdoor salt tolerant is identified II: will plant in seashore geavy salt alkaline earth through the experiment material that method (1), (2), (3) all are accredited as the salt tolerant germplasm and finally screen, method (4) is all selected to identify in whole growing, in like manner, outdoor salt tolerant identifies that II and outdoor salt tolerant evaluation I can adopt one or employing simultaneously separately.
The salt tolerant germplasm planted in the Hua Feng farm in 2008, the Hua Feng farm is positioned at several kilometers places, Da Feng harbor district southern side, it is typical strongly salined soil, saliferous reaches 0.4%-0.8%, identify, screen, the result: most of seeds can not be emerged, and select salt resistance strong from the plant of the upright seedling of small part, the good plant of yielding ability is selected fine individual plant salt 1032, salt 1129 as the salt tolerant strain.
2. strongly salined soil is identified relatively
Carried out the seashore strongly salined soil in 2009 and identify (＞0.4%), expert evidence is a salt 1032,1129,2018, contrast: cotton No. 9 of Soviet Union, anti-No. 1 of nasal mucus.This beach soil belongs to the marine solonchak type, and P in soil H value is about 8.40, and the content of organic matter is about 0.65%, total nitrogen content 0.05%, rapid available phosphorus 2.74ppm, available potassium 496.12ppm.According to salt content and fertility level, this saline land is the moderate salinization soil.From the result, symptom of a trend product tie up to aspects such as growing way, appearance, output, resistance and contrast.
Full-bloom stage sees Table 4 to salt tolerant strain salt 1032, salt 1129, salt 2018, the anti-No. 1 investigation tree characteristics of contrast nasal mucus: result's statistics all is better than contrast for the salt tolerant strain, and better with salt 1032, salt 1129.
The investigation of table 4 full-bloom stage proterties
Annotate: skellat counts the individual plant bell by 1/2, and flower, flower bud count the individual plant bell by 1/3.
3. salt tolerant kind (being) demonstration plant
2010 Hua Feng farm demonstration plant salt tolerant strain salt 1032, salt 1129 each more than 100 mu, the term of opening bolls cotton survey for No. 9 of salt 1032, salt 1129 and contiguous field piece Soviet Union produced statistics.Surveying the product result shows: salt tolerant strain salt 1032 still reaches 3822kg year with great calamity per hectare unginned cotton in 2009, and salt 1129 per hectare unginned cottons reach 3469.9kg.Contrast Soviet Union is 2671.6kg.hm-2 cotton No. 9, and salt 1032, salt 1129 are compared respectively according to volume increase 43.07%, 29.88%, the results are shown in Table 5.
Data are produced in table 5 survey in the term of opening bolls
Should be understood that, for those of ordinary skills, can be improved according to the above description or conversion, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (5)
1. the salt-enduring species matter authentication method that grows cotton is characterized in that, adopts laboratory experiment and outdoor experiment in conjunction with carrying out the screening of salt-enduring species matter, and described laboratory experiment comprises indoor germination experiment I and/or indoor germination experiment II; Described outdoor experiment comprises outdoor salt tolerant evaluation I and/or outdoor salt tolerant evaluation II.
2. method according to claim 1, it is characterized in that, described indoor germination experiment I is double-deck filter paper method, way is: with a filter paper pads of having soaked 0.4% salt solution below the plastics grid, the seed that will soak 0.4% salt solution again is placed on the filter paper, and then covers the filter paper that one deck soaked 0.4% salt solution.
3. method according to claim 1, it is characterized in that, described indoor germination experiment II adopts " Sha Peifa " to identify, described " Sha Peifa " adopts the square plastic container to pack into after fine sand germinates earlier by test material, the salt that claims fine sand weight 0.4% again, the water of fine sand weight 23% stirs evenly the back and imports in the container, culture environment is the artificial culture case, and temperature is 30 degree Celsius.
4. method according to claim 1 is characterized in that, described outdoor salt tolerant identifies that I is the salt pond method: will salt resistance be reached the above material of anti-level proceed the salt pond evaluation through after indoor germination experiment I and/or the indoor germination experiment II.
5. method according to claim 1, it is characterized in that, described outdoor salt tolerant identifies that II is a seashore geavy salt alkaline earth method, will identify that experiment material that I is accredited as the salt tolerant germplasm is planted in seashore geavy salt alkaline earth through indoor germination experiment I and/or indoor germination experiment II, outdoor salt tolerant and finally screen.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613017A (en) * | 2012-03-04 | 2012-08-01 | 中国农业科学院棉花研究所 | Identification method for salt resistance of cotton blades |
| CN103392405A (en) * | 2013-07-04 | 2013-11-20 | 甘肃农业大学 | Simple screening method for salt-tolerance maize inbred lines |
| CN103828713A (en) * | 2014-02-25 | 2014-06-04 | 江苏沿海地区农业科学研究所 | Saline-alkaline tolerant germplasm screening and high-yield cultivating method of barley |
| CN105353089A (en) * | 2015-11-13 | 2016-02-24 | 江苏省农业科学院 | An evaluating method of cotton variety saline tolerance |
| CN111011208A (en) * | 2019-12-06 | 2020-04-17 | 江苏沿海地区农业科学研究所 | Method for screening salt-tolerant cotton varieties |
| CN111656905A (en) * | 2020-07-06 | 2020-09-15 | 中国农业科学院棉花研究所 | Na-resistant cotton2SO4Method of (3) identification |
| CN112273222A (en) * | 2020-10-22 | 2021-01-29 | 河北省农林科学院旱作农业研究所 | Cotton variety breeding method |
-
2011
- 2011-02-21 CN CN2011100411518A patent/CN102172118A/en active Pending
Non-Patent Citations (1)
| Title |
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| 李寒暝: "耐盐棉花品种筛选", 《新疆农业大学硕士学位论文》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613017A (en) * | 2012-03-04 | 2012-08-01 | 中国农业科学院棉花研究所 | Identification method for salt resistance of cotton blades |
| CN103392405A (en) * | 2013-07-04 | 2013-11-20 | 甘肃农业大学 | Simple screening method for salt-tolerance maize inbred lines |
| CN103392405B (en) * | 2013-07-04 | 2015-07-15 | 甘肃农业大学 | Simple screening method for salt-tolerance maize inbred lines |
| CN103828713A (en) * | 2014-02-25 | 2014-06-04 | 江苏沿海地区农业科学研究所 | Saline-alkaline tolerant germplasm screening and high-yield cultivating method of barley |
| CN105353089A (en) * | 2015-11-13 | 2016-02-24 | 江苏省农业科学院 | An evaluating method of cotton variety saline tolerance |
| CN111011208A (en) * | 2019-12-06 | 2020-04-17 | 江苏沿海地区农业科学研究所 | Method for screening salt-tolerant cotton varieties |
| CN111656905A (en) * | 2020-07-06 | 2020-09-15 | 中国农业科学院棉花研究所 | Na-resistant cotton2SO4Method of (3) identification |
| CN112273222A (en) * | 2020-10-22 | 2021-01-29 | 河北省农林科学院旱作农业研究所 | Cotton variety breeding method |
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Application publication date: 20110907 |