CN102165916B - Solid substrate priming method of seeds of seedless watermelon - Google Patents
Solid substrate priming method of seeds of seedless watermelon Download PDFInfo
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- CN102165916B CN102165916B CN201110113494A CN201110113494A CN102165916B CN 102165916 B CN102165916 B CN 102165916B CN 201110113494 A CN201110113494 A CN 201110113494A CN 201110113494 A CN201110113494 A CN 201110113494A CN 102165916 B CN102165916 B CN 102165916B
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Abstract
The invention discloses a solid substrate priming method of seeds of a seedless watermelon, relating to a seed treatment technology and comprising the following six steps of: firstly, selecting and pretreating a solid substrate, secondly, selecting and treating seeds, thirdly, priming the seeds, fourthly, separating the seeds from sawdust, fifthly, drying, and sixthly, storing the seeds at a low temperature. The method for conductivity measurement or activity measurement of dehydrogenase is adopted when the seeds are selected according to needs, the seeds with poor innate vigor are removed to avoid unnecessary priming waste. In the method, sawdust is used as the solid substrate, therefore, the material is easily available, the cost is low, the specific gravity and the friction coefficient are low, the operation is relatively convenient, the surface of a screen is less worn when in screening or separating, the seeds are cultured in darkness in an incubator so that electric power is saved, the priming effect of seeds is extremely ideal, and the positive effect on seed germination is superior to the priming effect of other solid substrates.
Description
Technical field
The present invention relates to a kind of seed processing technology, especially make solid matrix improves germination rate and vigor to triploid watermelon seed initiating method with wood sawdust.
Background technology
CN101422098 discloses Xibei Univ. of Agricultural & Forest Science & Technology's " a kind of method that improves triploid watermelon seed germination rate and planting percent ", with wet towel parcel watermelon seed or with perlite companion's water and watermelon seed mixing, in illumination box, causes.CN101828443 has announced Beijing City Agriculture and Forestry Institute " a kind of solid matrix initiating method that improves watermelon seed germination rate and vigor "; Use vermiculite and water to press mass ratio and be (3-5): (2-3) mix; The mass ratio of seed and solid matrix is (30-40): (240-250); The container of packing into after vermiculite, water and seed are mixed thoroughly, 24h and 30h are cultivated in sealing in 20 ℃.Solid matrix commonly used also has sand, openly uses the seed initiating method of sand as solid matrix like CN1475096.In a word, the useful vermiculite of solid matrix of the prior art or perlite or sand, these solid matrixs are than great; Coefficient of friction is big, selects materials when separating with sieve, and is more serious to the screen cloth wearing and tearing; Operation is effort, and the size of vermiculite and perlitic particle diameter and watermelon seed is approaching when in clear water, separating seed and solid matrix, and the shape that is prone to harden on the screen cloth face of sand in water gathers; Separate and also to owe convenient, and vermiculite and perlite price are higher.Do not find as yet to make the document announcement that solid matrix causes seed through retrieval with wood sawdust.
Summary of the invention
The technical problem that the present invention will solve provides a kind ofly makes the method that solid matrix causes the aspermous watermelon seed with wood sawdust.
The technical measures that solve the problems of the technologies described above are: the solid matrix initiating method of this aspermous watermelon seed carries out as follows:
(1) selection of solid matrix and preliminary treatment: select for use after screening particle diameter be 8-10 purpose wood sawdust as solid matrix, with after water mixes, together as the solid matrix initator, the moisture content of this initator is 51%-69% again;
(2) selection of seed and processing: select the unabroken new seed of full grains; Is 1 with the wood sawdust of seed and moisture content 51%-69% by its weight ratio: mixed (1-3) is even; Place the interior 2/3-4/5 of airtight initiation box to this box volume; All seeds are the initator all standing, cover lid, make to be in sealing state;
(3) initiation of seed: will place incubator through the initiation box that step (2) dispose, and take out thing in the box in 20 ℃ of constant temperature behind dark culturing 24h or 36h or the 48h;
(4) separating of seed and wood sawdust: pour thing in the box into 8-10 purpose sieve, in clear water, sieve, seed and wood sawdust are separated;
(5) drying: dry 22-26h under 25 ℃ of temperature is returned to original weight to seed weight with drying box;
(6) low temperature storage: storage was sowed in≤30 days under 4 ℃ cryogenic conditions.
Said initator moisture content is 60%, and seed and moisture content are that the wood sawdust of 51%-69% is that 1: 2 mixed is even by its weight ratio.
Said seed selects to adopt the DDS307 conductivity meter that seed is carried out conductance measurement, the seed of electrical conductivity EC>=300 μ S.cm-1.g-1 need not, or seed carried out measuring desaturase liveness, the seed of its OD490nm≤1.0 need not.
Effective effect of the present invention is: the wood sawdust solid matrix material of employing is easy to get; Cost is low; And little because of wood sawdust proportion, coefficient of friction is low, operation is light, little to the wearing and tearing of sieve wire side; Again when causing in incubator dark culturing, energy-conservation, the weak effect that the effect that seed is caused causes seed unlike other solid matrix.
Embodiment
The present invention below will combine embodiment to make further detailed description:
Embodiment 1: selecting for use and mixing into moisture content thoroughly through the wood sawdust of 8 order aperture sieve and water is 51% solid matrix initator (hereinafter to be referred as initator); Select the full unabroken aspermous watermelon new seed of the previous year, also need in case of necessity this seed is carried out conductance measurement or measuring desaturase liveness, select the seed of electrical conductivity EC＜300 μ S.cm-1.g-1 or dehydrogenase activity OD490nm＞1.0 for use; Reserve a small amount of initator; Again with seed and remaining initator by weight being 1: 1 mixing, the initator of the thick reserved part of layer overlay 2cm at the bottom of causing box earlier, the initator that will be mixed with seed again places to cause in the box and paves; The initator of the reservation that layer overlay 1cm is thick above that again; Account for 2/3 of this box cubical content together, it is airtight to close the lid, and places incubator; Pouring 8 mesh into behind the dark culturing 24h in 20 ℃ of constant temperature sieves in clear water; Separate seed and wood sawdust, place drying box after 20 ℃ of dry about 22h return back to original weight to seed weight in seed, be no more than sowing in 30 days 4 ℃ of following preservations again.
Embodiment 2: remove select for use through the wood sawdust of 9 order aperture sieve and water mix thoroughly to moisture content be 60% initator, seed and initator by weight be 1: 2 mixing, place in the initiation box to this box cubical content 3/4, at insulating box dark culturing 36h, at the about 24h of drying box drying, all the other and embodiment 1 same procedure are carried out.
Embodiment 3: remove select for use through the wood sawdust of 10 order aperture sieve and water mix thoroughly to moisture content be 69% initator, seed and initator by weight be 1: 3 mixing, place in the initiation box to this box cubical content 4/5, at insulating box dark culturing 48h, at the about 26h of drying box drying, all the other and embodiment 1 same procedure are carried out.
In the actual production because of the land for growing field crops; The success or failure of sowing; Directly have influence on the harvest of annual aspermous watermelon, can not adopt a casual attitude, seed is carried out conductance measurement or measuring desaturase liveness also adopting the DDS307 conductivity meter in the selection of seed; To reject electrical conductivity EC>=300 μ S.cm-1.g-1 or low seed of these congenital vigor of dehydrogenase activity OD490nm≤1.0; Because of though these seeds can eventually because of inadequate natural endowment influences the output that the land for growing field crops produces, and say that to a certain extent to its initiation be a kind of waste through causing a little vigor that improves.
Following the present invention will be an example with the blue or green No. 1 aspermous watermelon seed in Shandong, make solid matrix with perlite and wood sawdust respectively, and cultivation is to the comparative test result announcement of the influence of seed germination as follows under the adverse circumstance temperature of 25 ℃ preference temperatures and 10 ℃ and 35 ℃:
Perlite is caused blue or green No. 1 seed in Shandong at present under preference temperature table 1 is listed in the influence of its sprouting:
Table 1 perlite cause to the Shandong blue or green No. 1 under the preference temperature condition (25 ℃/24h) influence of seed germination
Annotate: the different letter representation significant differences (p＜0.05) of same column, least significant difference method (LSD) is analyzed
Can find out that from table 1 perlite causes the sprouting that has promoted blue or green No. 1 seed in Shandong under the preference temperature condition, germination vigor, germination rate, germination index, vitality index, regularity all make moderate progress.Concerning germination vigor, 36h and the initiation of 48h perlite have significantly improved germination vigor, have improved 190% and 60% respectively, and 12h and 24h perlite initiation significantly the reduction on the contrary germination vigor.Concerning germination rate, the initiation of 48h perlite has significantly improved germination rate, has improved 100%, and 12h, 24h and 36h perlite initiation back percentage of seedgermination are not significantly improved.Concerning germination index, 36h and the initiation of 48h perlite have significantly improved germination index, improved 70% and 110% respectively, and 12h and 24h perlite initiation back seed sprouting index are not significantly improved.Concerning vitality index, 24h, 36h, 48h perlite cause back seed vitality index and all are significantly improved, and have improved 70%, 160%, 190% respectively, do not have marked change but the 12h perlite causes the vitality index and the contrast of back seed.Degree of aligning, four kinds of perlite initiating methods have all reduced the coefficient of variation, and wherein 48h perlite initiation effect is the most obvious, has reduced by 60%.
Table 2 perlite cause to the Shandong blue or green No. 1 under the adverse circumstance temperature condition (10 ℃/12h, 35 ℃/12h) influence of seed germination
Annotate: the different letter representation significant differences (p＜0.05) of same column, least significant difference method (LSD) is analyzed
Can find out that from table 2 perlite causes the sprouting that has promoted blue or green No. 1 seed in Shandong under the adverse circumstance temperature condition, germination vigor, germination rate, germination index, vitality index, regularity all make moderate progress.Concerning germination vigor, 12h, 24h, 36h, 48h perlite cause the germination vigor that has been significantly increased, and have improved 170%, 210%, 150%, 270% respectively.Concerning germination rate, 24h and the initiation of 48h perlite have significantly improved germination rate, improved 30% and 40% respectively, and 12h and 36h perlite initiation back percentage of seedgermination are not significantly improved.Concerning germination index, 24h and the initiation of 48h perlite have significantly improved germination rate, improved 30% and 40% respectively, and 12h and 36h perlite initiation back percentage of seedgermination are not significantly improved.Concerning vitality index, 24h, 36h, 48h perlite cause back seed vitality index and all are significantly improved, and have improved 50%, 40%, 50% respectively, do not have marked change but the 12h perlite causes the vitality index and the contrast of back seed.Degree of aligning, four kinds of perlite initiating methods have all reduced the coefficient of variation, and wherein 48h perlite initiation effect is the most obvious, has reduced by 40%.
Table 3 sawdust cause to the Shandong blue or green No. 1 under the preference temperature condition (25 ℃/24h) influence of seed germination
Annotate: the different letter representation significant differences (p＜0.05) of same column, least significant difference method (LSD) is analyzed
Can find out that from table 3 sawdust causes the sprouting that has promoted blue or green No. 1 seed in Shandong under the preference temperature condition, germination vigor, germination rate, germination index, vitality index, regularity all make moderate progress.Concerning germination vigor, the initiation of 24h sawdust has significantly improved germination vigor, has improved 190%, and 36h and the initiation of 48h sawdust all do not significantly improve germination vigor, and the 12h sawdust causes the back potentiality of seed and significantly reduces, and does not see seed germination.Concerning germination rate, 36h and the initiation of 48h sawdust have significantly improved germination rate, improved 90% and 80% respectively, and 12h and 24h sawdust initiation back percentage of seedgermination are not seen marked change.Concerning germination index, 24h, 36h, the initiation of 48h sawdust have significantly improved germination index, improved 60%, 90%, 100% respectively, and 12h sawdust initiation back seed sprouting index have reduced, but have not had marked change.Concerning vitality index, 24h, 36h, 48h sawdust cause back seed vitality index and all are significantly improved, and have improved 160%, 140%, 160% respectively, have reduced but the 12h sawdust causes the vitality index of back seed, but have not had marked change.Degree of aligning, four kinds of sawdust initiating methods have all reduced the coefficient of variation, and wherein 36h sawdust initiation effect is the most obvious, has reduced by 50%.
Table 4 sawdust cause to the Shandong blue or green No. 1 under the adverse circumstance temperature condition (10 ℃/12h, 35 ℃/12h) influence of seed germination
Annotate: the different letter representation significant differences (p＜0.05) of same column, least significant difference method (LSD) is analyzed
Can find out that from table 4 sawdust causes the sprouting that has promoted blue or green No. 1 seed in Shandong under the adverse circumstance temperature condition, germination vigor, germination rate, germination index, vitality index, regularity all make moderate progress.Concerning germination vigor, four kinds of initiations germination vigor that has been significantly increased has improved 140%, 170%, 350%, 310% respectively.Concerning germination rate, 36h and the initiation of 48h sawdust have significantly improved germination rate, all improved 40%, and 12h and 24h sawdust initiation back percentage of seedgermination are not seen marked change.Concerning germination index, 36h, 48h sawdust cause and have significantly improved germination index, have improved 60%, 50% respectively, and 12h, the 24h sawdust causes back seed sprouting index does not have marked change.Concerning vitality index, 36h, 48h sawdust cause back seed vitality index and all are significantly improved, and have improved 80%, 60% respectively, do not have marked change but 12h and 24h sawdust cause the vitality index of back seed.Degree of aligning, four kinds of sawdust initiating methods have all reduced the coefficient of variation, and wherein 24h sawdust initiation effect is the most obvious, has reduced by 50%.
Under the preference temperature condition, blue or green No. 1 seed in the Shandong after wood sawdust causes is being more or less the same aspect germination vigor, germination rate, germination index, vitality index and the regularity.But under the adverse circumstance temperature condition, except that germination rate and regularity difference not quite, the improvement degree that wood sawdust causes germination vigor to seed, germination index, vitality index is significantly higher than perlite and causes.Above presentation of results, wood sawdust cause poor unlike the perlite initiation, even the improvement to blue or green No. 1 seed vitality in Shandong has better effect under the adverse circumstance temperature condition.Make a general survey of all the other and make solid matrix with sand, vermiculite and compare, similar situation is also arranged with wood sawdust.
Seed vitality is high more, and its cell membrane system integrality is good more, and the seed inclusion is exosmosed just few more, and electrical conductivity is just low more.Seed vitality is high more, and respiratory metabolism is vigorous more, when life seed is breathed, since the dehydrase effect, matrix generation certain embodiments, thereby make colourless triphenyltetrazolium chloride (TTC) be reduced to red material, can judge the dehydrogenase activity size according to the OD value.Therefore, need carry out the height that conductance measurement or ldh assay are judged seed vitality, reject the seed of congenital vigor difference, in order to avoid cause unnecessary initiation waste.
Claims (4)
1. the solid matrix initiating method of an aspermous watermelon seed is characterized in that carrying out as follows:
(1) selection of solid matrix and preliminary treatment: select for use after screening particle diameter be 8-10 purpose wood sawdust as solid matrix, with after water mixes, together as the solid matrix initator, the moisture content of this initator is 51%-69% again;
(2) selection of seed and processing: select the unabroken new seed of full grains; Is 1 with the wood sawdust of seed and moisture content 51%-69% by its weight ratio: mixed (1-3) is even; Place the interior 2/3-4/5 of airtight initiation box to this box volume; All seeds are the initator all standing, cover lid, make to be in sealing state;
(3) initiation of seed: will place incubator through the initiation box that step (2) dispose, and take out thing in the box in 20 ℃ of constant temperature behind dark culturing 24h or 36h or the 48h;
(4) separating of seed and wood sawdust: pour thing in the box into 8-10 purpose sieve, in clear water, sieve, seed and wood sawdust are separated;
(5) drying: dry 22-26h under 25 ℃ of temperature is returned to original weight to seed weight with drying box;
(6) low temperature storage: storage was sowed in≤30 days under 4 ℃ cryogenic conditions.
2. the solid matrix initiating method of aspermous watermelon seed as claimed in claim 1 is characterized in that said initator moisture content is 60%.
3. the solid matrix initiating method of aspermous watermelon seed as claimed in claim 1 is characterized in that said seed and moisture content are that the wood sawdust of 51%-69% is that 1: 2 mixed is even by its weight ratio.
4. the solid matrix initiating method of aspermous watermelon as claimed in claim 1 is characterized in that said seed selection employing DDS307 conductivity meter carries out conductance measurement to seed, electrical conductivity EC>=300 μ S.cm
-1.g
-1Seed need not, or seed carried out measuring desaturase liveness, its OD
490nm≤1.0 seed need not.
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| CN102165916B true CN102165916B (en) | 2012-09-05 |
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| CN105123026A (en) * | 2015-09-25 | 2015-12-09 | 镇江瑞繁农艺有限公司 | Treatment method of seedless watermelon seeds |
| CN109005980A (en) * | 2018-07-18 | 2018-12-18 | 安徽德昌苗木有限公司 | A kind of grafting method of camellia bud stocks |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101422098A (en) * | 2008-12-03 | 2009-05-06 | 西北农林科技大学 | Method for increasing germination ratio and seedling ratio of triploid watermelon |
| CN101828443A (en) * | 2010-04-23 | 2010-09-15 | 北京市农林科学院 | Solid matrix initiating method for improving germination rate and vitality of watermelon seeds |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101422098A (en) * | 2008-12-03 | 2009-05-06 | 西北农林科技大学 | Method for increasing germination ratio and seedling ratio of triploid watermelon |
| CN101828443A (en) * | 2010-04-23 | 2010-09-15 | 北京市农林科学院 | Solid matrix initiating method for improving germination rate and vitality of watermelon seeds |
Non-Patent Citations (2)
| Title |
|---|
| 李磊等.引发剂结合固体基质引发对三倍体西瓜种子发芽性能的影响.《作物研究》.2010,第24卷(第3期),第164-167页. * |
| 郑晓鹰等.固体基质引发西瓜种子的效果及其对β-半乳甘露聚糖酶活性和DNA复制的影响.《中国农业科学》.2009,第42卷(第3期),第951-959页. * |
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Effective date of registration: 20210826 Address after: Room 1011, 4 / F, building 5, No. 299, Hangfu Road, Chongfu Town, Tongxiang City, Jiaxing City, Zhejiang Province Patentee after: Tongxiang Beite Technology Co.,Ltd. Address before: 311300 No. 88 North Ring Road, Zhejiang, Ling'an Patentee before: ZHEJIANG A & F University |
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