CN102154142B - Microorganism for promoting black nightshade to remove trace cadmium pollution in water and method for removing cadmium pollution - Google Patents
Microorganism for promoting black nightshade to remove trace cadmium pollution in water and method for removing cadmium pollution Download PDFInfo
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- CN102154142B CN102154142B CN2010105131400A CN201010513140A CN102154142B CN 102154142 B CN102154142 B CN 102154142B CN 2010105131400 A CN2010105131400 A CN 2010105131400A CN 201010513140 A CN201010513140 A CN 201010513140A CN 102154142 B CN102154142 B CN 102154142B
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Abstract
The invention provides a microorganism for promoting black nightshade to remove trace cadmium pollution in water and a method for removing cadmium pollution, aiming to purify drinking water subjected to cadmium pollution by virtue of the high-efficiency absorption characteristic of super-accumulated plants, namely black nightshade, to cadmium. In the method, the black nightshade is cultured in an aquaculture manner, and Chryseobacterium sp. LKS03 is inoculated to the black nightshade to promote the roots of the black nightshade to grow; and during purification of drinking water, the roots of black nightshade plants are immersed into the drinking water subjected to cadmium pollution, and each black nightshade plant correspondingly purifies about 1-1.2L of the drinking water in a purification cycle, wherein the purification time is for 2-12h according to the cadmium ion concentration (not higher than 200mug/L) in a water sample, and the capability of the black nightshade plants for purifying the water sample is optimum in the 45th-80th day in the culture cycle of the black nightshade. The method is specific to the cadmium pollution in the drinking water and has the advantages of good removal effect, simplicity and convenience for operation, wide applicable range, and great application potential in the aspect of drinking water purification.
Description
Technical field
The present invention relates to the biology purifying method of Trace Cadmium polluted drinking water, be specifically related to utilize black nightshade to remove the method for Trace Cadmium pollution in the water and the mikrobe of employing thereof.
Background technology
The generation of the environmental pollution that industrial development is often followed, the heavy metal pollution of water body problem that the waste water and dregs discharging in " three industrial wastes " causes is more and more serious, not only destroys ecotope, and directly or indirectly endangers the healthy of people.Heavy metal cadmium mainly from industries such as printing and dyeing, agricultural chemicals, ore extraction, smeltings, is taken in Trace Cadmium for a long time, organs such as the liver of meeting heavy damage human body, kidney, and then cause multiple disease.Europe and the itai-itai disease of Japan because of the cadmium pollution initiation are common diseases of cadmium pollution zone cadmium poisoning.
The method of cadmium pollution mainly contains in the removal tap water commonly used: flocculation agent filtration method, lime precipitation, ion exchange method, electrolytic process etc., but these methods exist the generation secondary pollution in treating processes, defectives such as expense is too high, reuse is strong, complex process.Therefore work out a kind of efficient, environmental protection, low cost method is the trend of present development in science and technology, the biology purifying method that the present invention adopts will solve a series of problems that existing method occurs.
Summary of the invention:
The technical problem that the present invention will solve is the deficiency that overcomes prior art, provides a kind of and promotes black nightshade to remove the mikrobe that Trace Cadmium is polluted in the water and remove the cadmium pollution method, and this method is simple to operate, economical and efficient, environmental friendliness.
A kind of mikrobe that promotes that Trace Cadmium is polluted in the black nightshade removal water is golden yellow bacillus Chryseobacterium sp.LKS03, and preserving number is CCTCC NO:M 2010236.
Use described mikrobe and promote black nightshade to remove the method that Trace Cadmium is polluted in the water,, take out sowing, change water planting over to after two weeks in golden yellow bacillus Chryseobacterium sp.LKS03 bacterium liquid, soaking 1-3 hour behind the black nightshade seed surface sterilization; Black nightshade plant after 30 days is used to purify waste water.
Described black nightshade seed soaks through 75% spirituous solution and carried out surface sterilization in 15 seconds, and flushing with clean water 3 times is used for endophyte bacterium liquid and soaks after drying.
Described bacterium liquid layoutprocedure is following: enlarged culturing is 12 hours in the golden yellow bacillus Chryseobacterium of picking sp.LKS03 bacterial classification inoculation to the 100ml liquid LB substratum; 4000 rev/mins centrifugal 5 minutes; Remove supernatant; With the resuspended bacterium thalline of the improvement Hoagland solution of 100ml 1/4 concentration, promptly obtain endophyte inoculation bacterium liquid.
Described sowing is that vermiculite is laid in the pallet, thickness 4-6 centimetre, irrigate with the improvement Hoagland solution of 1/4 concentration, and black nightshade seed is seeded in wherein.
Described water planting is to adopt the improvement Hoagland solution of 1/4 concentration as mill water culture nutrient solution, changes one time of nutrition liquid in 4-6 days, and constantly replenishes.
The improvement Hoagland solution prescription of described 1/4 concentration is: KNO
3125mg/L; KH
2PO
435mg/L; NH
4H
2PO
420mg/L; MgSO
47H
2O 125mg/L; Ca (NO
3)
24H
2O 235mg/L; FeSO
47H
2O 10mg/L.
The time that described black nightshade plant is used to purify waste water is 30-40 days.
Described black nightshade plant root contacts with water body purifies waste water.
Described black nightshade is used to handle concentration of cadmium ions for not being higher than the tap water of 200 μ g/L; After described black nightshade plant is taken turns clean water treatment in 2-12 hour through one, continue water planting and cultivated 24 hours, black nightshade is transported to blade and the preservation of stem stalk with the cadmium metal that major part absorbs from root, recovers the water purification ability, can carry out the next round clean water treatment.The corresponding 1-1.2 that purifies of every strain black nightshade rises the water of cadmium pollution.
Water sample all reaches the Drinking Water water quality standard (GB5749-85) that country formulates after treatment, generally all is lower than 3 μ g/L.
Black nightshade is accomplished the processing behind the circulation water purification: the inner cadmium that is absorbed of black nightshade plant can extract through the mode of mechanical expression, and extraction yield is near 80%; Plant residue after the squeezing is through oven dry, pulverizing, and the heavy metal absorption that can be used for trade effluent is removed.
It is good, easy and simple to handle, applied widely to the present invention is directed to the removal effect that Trace Cadmium is polluted in the tap water, aspect drink water purifying, has very big application potential.
Near the black nightshade plant that strain separating is gathered tailings ore deposit, mining area, mountain, the mouth of a river, hengyang, hunan province is the black nightshade endophyte.The whole strain black nightshade plant of gathering is clean with flushing with clean water, get part root, stem, leaf respectively and in the hypochlorous acid solution of 2% (v/v), soaks and be used for surface sterilization in 3 minutes, then the residual hypochlorous acid of rinsing 5 removals plant surface in sterilized water.Plant behind the surface sterilization is ground to pasty state with aseptic mortar,, on solid LB substratum plane, cultivates 48 hours with 10-10000 times of separate application of 1% (m/v) sodium-chlor dilution.Difference picking endophyte list bacterium colony according to colonial morphology carries out enlarged culturing.After detecting, chosen the endophyte that is numbered LKS03 and carried out the plant inoculation through heavy metal tolerance, the promoting growth of plants factor etc.LKS03 has the ability of higher production ACC (1-amino-cyclopropane-1-carboxylic acid) desaminase and IAA (indolylacetic acid), and its growth does not receive obviously to influence under the culture condition of Cd 2mM, Zn5mM, Pb 5mM, and spawn activity is normal.
LKS03 is a gram-positive microorganism, and suitable growth temperature is 25-30 ℃, be higher than 38 ℃ then the growth obviously be suppressed.After 2 days, its colony diameter is 1-1.5mm in coating on the solid LB substratum, 30 ℃ of cultivations, is yellow, smooth surface, convexity, moistening.According to the 16SrDNA sequence sequencing result (GenBank ID:HQ331133) of LKS03,, show that it is the bacterium of golden yellow Bacillaceae, with the golden yellow bacillus Chryseobacterium of its called after sp.LKS03 in conjunction with colonial morphology and physiological property analysis.
Description of drawings
Fig. 1 purifies the simulating pollution tap water time plot of different cadmium concentrations for black nightshade;
Fig. 2 is the black nightshade water purification time plot of culture cycle the 45th day and 80 days;
Fig. 3 is the black nightshade of the golden yellow bacillus Chryseobacterium sp.LKS03 of inoculation and the comparison of nonvaccinated black nightshade water purification ability;
Continuous 4 the clean water treatment design sketchs of the black nightshade of Fig. 4 golden yellow bacillus Chryseobacterium sp.LKS03 for the present invention has inoculated;
Fig. 5 has inoculated the influence of the black nightshade water purification ability of golden yellow bacillus Chryseobacterium sp.LKS03 to the present invention for several metal ion species.
Embodiment
Be intended to further specify the present invention below in conjunction with embodiment, and unrestricted the present invention.
Embodiment 1
The water planting of the sprouting of black nightshade seed, the golden yellow bacillus Chryseobacterium sp.LKS03 of inoculation and plant is cultivated:
1. black nightshade seed soaks through 75% spirituous solution and carried out surface sterilization in 15 seconds, and flushing with clean water 3 times is used for golden yellow bacillus Chryseobacterium sp.LKS03 inoculation after drying.
2. the black nightshade seed after will sterilizing was soaked in the golden yellow bacillus Chryseobacterium sp.LKS03 inoculation bacterium liquid 2 hours, took out promptly to be used for sowing.
3. vermiculite is laid in the pallet, and about 5 centimetres of thickness irrigates with the improvement Hoagland solution of 1/4 concentration, and black nightshade seed is seeded in wherein.Promptly germinate about 2-3 days, 2 all left and right sides black nightshades can grow to 10 centimetres high and change water planting over to.
4.1/4 the improvement Hoagland solution of concentration equally as mill water culture nutrient solution, changed one time of nutrition liquid in 5 days, and replenished frequently.Black nightshade plant after 30 days is used to purify waste water.
Chryseobacterium sp.LKS03 has the ability of higher production ACC (1-amino-cyclopropane-1-carboxylic acid) desaminase and IAA (indolylacetic acid), can promote the growth of plant root.At the 45th day and the 80th day of growth cycle, inoculated golden yellow bacillus Chryseobacterium sp.LKS03 plant and respectively gathered 5 strain samples with the plant that does not have inoculation, it is heavy to measure root length and root, and the result is as shown in table 2:
Table 2
The black nightshade plant that has inoculated golden yellow bacillus Chryseobacterium sp.LKS03 was at the 45th day of growth cycle, and root is long to be compared with the black nightshade that does not connect bacterium with the root weight, has increased by 21.8% and 25.8% respectively; At the 80th day, root is long and root is heavy then increased by 25.7% and 38.8% respectively.Experimental result shows that inoculation Chryseobacteriumsp.LKS03 has significant promoter action to the root growth of black nightshade.
Embodiment 3
Adopt tap water to add heavy metal ion and take the simulating pollution tap water, cadmium concentration is 50 μ g/L in the simulating pollution tap water, relatively connects the black nightshade of bacterium and does not connect the ability that the bacterium black nightshade purifies the simulating pollution tap water.1 liter of simulating pollution tap water injects tank; Black nightshade is fixed in the tank top; Root contacts with water body, follows the tracks of and detects the variation of cadmium concentration in the water body in 16 hours, and cadmium concentration is measured by atomic spectrophotometer; Purifying water effect is as shown in Figure 3: can be found out by experimental result; The black nightshade of inoculation Chryseobacteriumsp.LKS03 was reduced to (＜10 μ g/L) below the GB with cadmium content in the tap water in 3 hours, and the black nightshade that does not inoculate endophyte then needs 4.5 hours, and processing efficiency has improved nearly 33%.After this shows the inoculation endophyte, black nightshade root Absorbed Cadmium ionic speed is faster, and cleaning water is higher.
Adopt tap water to add heavy metal ion and take the simulating pollution tap water; Cadmium concentration is 100 μ g/L in the simulating pollution tap water; 1 liter of simulating pollution tap water injects tank, and the black nightshade that has inoculated golden yellow bacillus Chryseobacterium sp.LKS03 is fixed in the tank top, and root contacts with water body; Follow the tracks of and detect the variation of cadmium concentration in the water body in 16 hours, cadmium concentration is measured by atomic spectrophotometer.Black nightshade continued water planting after purifying treatment was accomplished, and 24 hours continued are carried out drink water purifying and handled.Carry out the drink water purifying in 4 cycles continuously and handle, purifying water effect is as shown in Figure 4: can be found out the continuous water purification ability quite stable of black nightshade by Fig. 4.
Embodiment 5
Adopt tap water to add heavy metal ion and take the simulating pollution tap water; Cadmium concentration is 100 μ g/L; Add several kinds of divalent metallic cations simultaneously respectively; Concentration is Zn 1000 μ g/L, Mn 500 μ g/L, Fe 1000 μ g/L, Ca 500mg/L, Mg 500mg/L, in order to detect the influence to the black nightshade water purification ability of having inoculated golden yellow bacillus Chryseobacterium sp.LKS03 of various common positively charged ions in the water.Follow the tracks of and detect the variation of cadmium concentration in the water body in 12 hours, cadmium concentration is measured by atomic spectrophotometer.Purifying water effect is as shown in Figure 5: can be found out that by Fig. 5 various metal divalent positively charged ions are very little to the influence of black nightshade water purification, black nightshade water purifying accommodation is very wide.
Claims (9)
1. one kind promotes black nightshade to remove the mikrobe that Trace Cadmium is polluted in the water, it is characterized in that described mikrobe is golden yellow bacillus Chryseobacterium sp.LKS03, and preserving number is CCTCC NO:M 2010236.
2. application rights requires 1 described mikrobe to promote black nightshade to remove the method that Trace Cadmium is polluted in the water; It is characterized in that; With in golden yellow bacillus Chryseobacterium sp.LKS03 bacterium liquid, soaking 1-3 hour behind the black nightshade seed surface sterilization, take out sowing, change water planting over to after two weeks; Black nightshade plant after 30 days is used to purify waste water.
3. mikrobe according to claim 2 promotes black nightshade to remove the method that Trace Cadmium is polluted in the water; It is characterized in that; Described black nightshade seed soaks through 75% spirituous solution and carried out surface sterilization in 15 seconds; Flushing with clean water 3 times is used for golden yellow bacillus Chryseobacterium sp.LKS03 bacterium liquid and soaks after drying.
4. mikrobe according to claim 2 promotes black nightshade to remove the method that Trace Cadmium is polluted in the water; It is characterized in that; Described bacterium liquid layoutprocedure is following: enlarged culturing is 12 hours in the golden yellow bacillus Chryseobacterium of picking sp.LKS03 bacterial classification inoculation to the 100ml liquid LB substratum, 4000 rev/mins centrifugal 5 minutes, remove supernatant; With the resuspended bacterium thalline of the improvement Hoagland solution of 100ml 1/4 concentration, promptly obtain bacterium liquid; The improvement Hoagland solution prescription of described 1/4 concentration is: KNO
3125mg/L; KH
2PO
435mg/L; NH
4H
2PO
420mg/L; MgSO
47H
2O 125mg/L; Ca (NO
3)
24H
2O 235mg/L; FeSO
47H
2O 10mg/L.
5. mikrobe according to claim 2 promotes black nightshade to remove the method that Trace Cadmium is polluted in the water; It is characterized in that described sowing is that vermiculite is laid in the pallet, thickness 4-6 centimetre; Improvement Hoagland solution with 1/4 concentration irrigates, and black nightshade seed is seeded in wherein; The improvement Hoagland solution prescription of described 1/4 concentration is: KNO
3125mg/L; KH
2PO
435mg/L; NH
4H
2PO
420mg/L; MgSO
47H
2O 125mg/L; Ca (NO
3)
24H
2O 235mg/L; FeSO
47H
2O 10mg/L.
6. mikrobe according to claim 2 promotes black nightshade to remove the method that Trace Cadmium is polluted in the water, it is characterized in that, described water planting is to adopt the improvement Hoagland solution of 1/4 concentration as mill water culture nutrient solution, changes one time of nutrition liquid in 4-6 days, and constantly replenishes; The improvement Hoagland solution prescription of described 1/4 concentration is: KNO
3125mg/L; KH
2PO
435mg/L; NH
4H
2PO
420mg/L; MgSO
47H
2O 125mg/L; Ca (NO
3)
24H
2O 235mg/L; FeSO
47H
2O 10mg/L.
7. mikrobe according to claim 2 promotes black nightshade to remove the method that Trace Cadmium is polluted in the water, it is characterized in that the time that described black nightshade plant is used to purify waste water is 30-40 days.
8. mikrobe according to claim 2 promotes black nightshade to remove the method that Trace Cadmium is polluted in the water, it is characterized in that described black nightshade plant root contacts with water body purifies waste water.
9. promote black nightshade to remove the method that Trace Cadmium is polluted in the water according to claim 2 or 7 or 8 described mikrobes, it is characterized in that described black nightshade is used to handle concentration of cadmium ions for not being higher than the tap water of 200 μ g/L; After described black nightshade plant is taken turns clean water treatment in 2-12 hour through one, continue water planting and cultivated 24 hours, carry out the next round clean water treatment, the corresponding 1-1.2 that purifies of every strain black nightshade rises the water of cadmium pollution.
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| CN102642931B (en) * | 2012-04-02 | 2013-09-18 | 常州亚环环保科技有限公司 | Synthesis of biological agent rich in phosphorous content in natural water resources and application thereof |
| CN103241839B (en) * | 2013-05-27 | 2014-12-03 | 沈阳大学 | Treatment method of treating cadmium contaminated water |
| CN106554086B (en) * | 2016-11-29 | 2018-09-14 | 郑州轻工业学院 | A kind of cadmium pollution food chain restorative procedure based on Pu Ye-fungi synergy |
| CN107282010A (en) * | 2017-07-03 | 2017-10-24 | 安徽天顺环保设备股份有限公司 | A kind of heavy metal absorbent |
| CN108658245A (en) * | 2018-04-30 | 2018-10-16 | 桂林理工大学 | A kind of preparation method of processing lead zinc waste water microbial flocculant |
| CN109105237A (en) * | 2018-06-26 | 2019-01-01 | 南京工程学院 | Remove and measure the experimental method that wetland plant rhizosphere microorganism group influences phosphorus in plant absorption water |
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