CN102146106A - Method for extracting 95 percent of rebaudioside A - Google Patents
Method for extracting 95 percent of rebaudioside A Download PDFInfo
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- CN102146106A CN102146106A CN2010101081262A CN201010108126A CN102146106A CN 102146106 A CN102146106 A CN 102146106A CN 2010101081262 A CN2010101081262 A CN 2010101081262A CN 201010108126 A CN201010108126 A CN 201010108126A CN 102146106 A CN102146106 A CN 102146106A
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Abstract
The invention relates to a method for extracting 95 percent of rebaudioside A, which belongs to the technical field of production of food additives. The rebaudioside A is a component having best taste in stevioside; and the traditional production process commonly has the defects of low conversion ratio, high energy consumption, high cost, unstable control on effective components, and the like due to difference of respective processes and methods. In the method, solvents such as methanol, ethanol, and the like are mixed according to a certain proportion; the mixture and 40 percent of RA (Rebaudioside A) are mixed according to a certain proportion; after the mixture is stirred at normal temperature, the mixture is placed and separated for carrying out solid and liquid separation; the obtained solid is dried or dried after washed to obtain high-purity RA; and the content of the RA can reach 95-99 percent, and the conversion ratio of the RA is more than 80 percent. The process for producing the high-purity RA has the advantages of simple process, low energy consumption, high yield, and the like and is suitable for industrialized production.
Description
Technical field
The invention belongs to the foodstuff additive production technical field.
Background technology
Steviosides is that a kind of high sugariness that extraction obtains from sweet Stevia is hanged down heat energy, flavor matter is good and the natural low-heat sugar source of safety non-toxic, and its sugariness is 150～300 times of sucrose, can substitute asccharin or sucrose, is applied in various food, the beverage.Steviosides can also prevent diseases such as diabetes, obesity and pediatric saprodontia, is patient's desirable sweeting agent.But the bitter taste of stevioside influences its popularization and utilization always, and Japan and Korea S adopt the method for transferring enzyme and lytic enzymeization to improve the bitter aftertaste of stevioside; Insufficient is the side effect sugariness multiple decline often that brings, cost up.Cause the difficulty in the application equally.But progressively increase along with the natural sweeteners market requirement, the back bitter taste that more and more enterprises begins to pay close attention to research and solves stevia rebaudianum, people attempt the stevia rebaudianum product of refining refinement Gao Laibaodi glycosides content, get rid of bad composition steviobioside of mouthfeel and Du Ke glucoside, sugariness can up to 350-400 doubly have been removed the back bitter taste of stevia rebaudianum simultaneously like this.At present, some enterprises of Korea S, Malaysia, the U.S., China etc. can be purified to the Lai Baodi glycosides more than 80%, but because the difference of technology and method separately, the ubiquity transformation efficiency is low, the energy consumption height, cost height, drawbacks such as effective constituent control instability.
Summary of the invention
In order to overcome the above problems, the invention provides the extracting method of a kind of 95% content Lai Baodi glycosides A, this method operational path is simple, and is not high to equipment requirements, with short production cycle, and has advantages such as less energy-consumption, low cost, low residue, is fit to suitability for industrialized production.
Concrete technical scheme is as follows, the extracting method of a kind of 95% content Lai Baodi glycosides A, and it comprises following step:
(1) the solvent preparatory stage, methyl alcohol, ethanol, water are pressed 2～5: 1～6: 0～3 mixed.
(2) mix stages: purity 40% above stevioside was mixed than 1: 2～1: 10 by plastid with mixed solvents such as methyl alcohol.
(3) agitation phases:, and keep 1～4h with the said mixture stirring at normal temperature.
(4) the placement stage: mixture after being mixed is placed 0～96h in the temperature below 30 ℃ RA is fully separated out.
(5) solid-liquid separation: carry out solid-liquid separation with centrifugal or filtering method, filter that aperture of filter material is less than 50 μ m or greater than 500 orders.
(6) method that isolating solid substance is done with oven dry or spray is carried out drying.
Embodiment
Embodiment 1
(1) the solvent preparatory stage, methyl alcohol, ethanol, water are pressed 3: 4: 3 mixed.
(2) mix stages: purity 40% above stevioside was mixed than 1: 4 by plastid with mixed solvents such as methyl alcohol.
(3) agitation phases:, and keep 2h with the said mixture stirring at normal temperature.
(4) the placement stage: mixture after being mixed is placed 72h in the temperature below 30 ℃ RA is fully separated out.
(5) solid-liquid separation: carry out solid-liquid separation with filtering method, filter filter material 500 purpose filter clothes.
(6) isolating solid substance is dried with infrared drying oven.
Embodiment 2
(1) the solvent preparatory stage, methyl alcohol, ethanol, water are pressed 4: 5: 1 mixed.
(2) mix stages: purity 40% above stevioside was mixed than 1: 2 by plastid with mixed solvents such as methyl alcohol.
(3) agitation phases:, and keep 4h with the said mixture stirring at normal temperature.
(4) the placement stage: mixture after being mixed is placed 48h in the temperature below 30 ℃ RA is fully separated out.
(5) solid-liquid separation: carry out solid-liquid separation with filtering method, filter filter material 500 purpose filter clothes.
(6) with isolating solid substance with deionized water dissolving after, volatilize organic solvent, the spray-drier drying.
Claims (1)
1. the extracting method of a content Lai Baodi glycosides A is characterized in that it comprises the following aspects:
(1) raw material: the raw material that this technology is used can be that RA content is at the steviol glycoside more than 40%.
(2) solvent: the solvent that this technology is used is a methyl alcohol, ethanol, water etc.
(3) ratio with the described solvent of claim (2) is a methyl alcohol: ethanol: water (2～5: 1～6: 0～3).
(4) be 2～6: 1 with described mixed solvent of claim (3) and the described material rate of claim (1), after raw material and the solvent, at stirring at normal temperature 1～4h.
(5) finish with the stirring under the claim (3), (4) after, mixed solution should placed 0～96h below 30 ℃.
(6) with the mixed solution under the claim (5), to place to separate out and finish the back and carry out solid-liquid separation with centrifugal or filtering method, the filtration aperture of filter material is less than 50 μ m or more than 500 orders.
(7) method that isolating solid substance is done with oven dry or spray is carried out drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101081262A CN102146106A (en) | 2010-02-10 | 2010-02-10 | Method for extracting 95 percent of rebaudioside A |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101081262A CN102146106A (en) | 2010-02-10 | 2010-02-10 | Method for extracting 95 percent of rebaudioside A |
Publications (1)
| Publication Number | Publication Date |
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| CN102146106A true CN102146106A (en) | 2011-08-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN2010101081262A Pending CN102146106A (en) | 2010-02-10 | 2010-02-10 | Method for extracting 95 percent of rebaudioside A |
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| CN (1) | CN102146106A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102775453A (en) * | 2012-06-21 | 2012-11-14 | 黑龙江八一农垦大学 | Method for crystallizing and separating stevioside A3 from stevia total glycoside mixture |
| CN104725444A (en) * | 2015-03-24 | 2015-06-24 | 杨健 | Method for efficiently preparing high-purity rebaudioside A |
| CN109456371A (en) * | 2018-10-16 | 2019-03-12 | 桂林莱茵生物科技股份有限公司 | A kind of preparation method of efficient steviol glycoside mixture |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008057967A2 (en) * | 2006-11-02 | 2008-05-15 | The Coca-Cola Company | High-potency sweetener composition with preservative and compositions sweetened therewith |
| CN101220062A (en) * | 2008-01-23 | 2008-07-16 | 石任兵 | Method for preparing stevioside and rebaudiodside A simultaneously |
| CN101472487A (en) * | 2006-06-19 | 2009-07-01 | 可口可乐公司 | Rebaudioside a composition and method for purifying rebaudioside a |
-
2010
- 2010-02-10 CN CN2010101081262A patent/CN102146106A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101472487A (en) * | 2006-06-19 | 2009-07-01 | 可口可乐公司 | Rebaudioside a composition and method for purifying rebaudioside a |
| WO2008057967A2 (en) * | 2006-11-02 | 2008-05-15 | The Coca-Cola Company | High-potency sweetener composition with preservative and compositions sweetened therewith |
| CN101220062A (en) * | 2008-01-23 | 2008-07-16 | 石任兵 | Method for preparing stevioside and rebaudiodside A simultaneously |
Non-Patent Citations (1)
| Title |
|---|
| 刘杰，等: "莱鲍迪A甙制备及其安全性的研究", 《安徽农业科学》, vol. 37, no. 2, 15 April 2009 (2009-04-15), pages 825 - 827 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102775453A (en) * | 2012-06-21 | 2012-11-14 | 黑龙江八一农垦大学 | Method for crystallizing and separating stevioside A3 from stevia total glycoside mixture |
| CN104725444A (en) * | 2015-03-24 | 2015-06-24 | 杨健 | Method for efficiently preparing high-purity rebaudioside A |
| CN109456371A (en) * | 2018-10-16 | 2019-03-12 | 桂林莱茵生物科技股份有限公司 | A kind of preparation method of efficient steviol glycoside mixture |
| WO2020077970A1 (en) * | 2018-10-16 | 2020-04-23 | 桂林莱茵生物科技股份有限公司 | Highly efficient stevioside mixture preparation method |
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Application publication date: 20110810 |