CN104388498A - Method for producing total glycoside 80% enzymes for improving stevioside taste quality - Google Patents
Method for producing total glycoside 80% enzymes for improving stevioside taste quality Download PDFInfo
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- CN104388498A CN104388498A CN201410540304.7A CN201410540304A CN104388498A CN 104388498 A CN104388498 A CN 104388498A CN 201410540304 A CN201410540304 A CN 201410540304A CN 104388498 A CN104388498 A CN 104388498A
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Abstract
The invention relates to a method for producing total glycoside 80% enzymes for improving the stevioside taste quality. The method includes the following steps: dissolving, stirring, pH value adjusting, adding of cyclodextrin glucosyltransferase, temperature adjusting, inactivating, filtering, adsorbing, water washing, alcohol washing, concentrating and atomizing drying. The method has the beneficial effects that molecular structures of stevioside are modified through the effect of the enzymes; the aim of improving the stevioside taste is achieved; peculiar smells are removed from obtained stevioside derivatives to obtain high-sweetness and low-heating-value natural sweetening agents; and the glucoside content of the product can reach more than 80%.
Description
Technical field
The invention belongs to production process of stevioside technical field, is more particularly the production method that a kind of general glycoside 80% enzyme improves taste of ribaudiose.
Background technology
Stevioside is the mixture of the diterpene glucosides containing 8 kinds of compositions extracted from the leaf of sweet Stevia, it is a kind of natural intense sweetener, its sugariness is 200 ~ 350 times of sucrose, the content of the various composition of stevioside, mouthfeel and sugariness are different, wherein the content of stevioside, rebaudioside A, dulcoside B is higher, accounts for more than 90% altogether.Rebaudioside A glycosides sugariness is high, and sweet taste characteristic is also close with sucrose, be desirable sweet ingredient, and both stevioside and dulcoside B accounts for 40 ~ 70% of stevioside, all has certain bitter taste and offending aftertaste, has had a strong impact on the taste matter of stevioside.But bitter taste after still existing when rebaudioside A glycosides content is purified and reached more than 98%, therefore independent raising rebaudioside A glycosides content can not solve the problem of bitter taste after stevioside product.
Summary of the invention
In order to solve the problem, the invention provides the molecular structure that stevioside is modified in a kind of effect by enzyme, reach the object improving stevioside mouthfeel, the stevioside derivative obtained eliminates peculiar smell and obtains high sugariness, low-calorie natural sweeteners, product glucoside content is made to reach more than 80%, its concrete scheme is: the production craft step that described general glycoside 80% enzyme improves taste of ribaudiose is: dissolve → stir → regulate pH value, add cyclomaltodextrin glucanotransferase, regulate temperature → deactivation, filter → absorption, washing, alcohol wash → concentrated, spraying dry,
Dissolving of the present invention: it is characterized in that adding certain water in retort, be warmed up to 55 ~ 60 degree, add maltodextrin, preparation maltodextrin solution, the ratio of maltodextrin and water selects 1:7 ~ 1:20;
Stirring of the present invention: it is characterized in that adding general glycoside 80% stevioside in maltodextrin solution, add the ratio of stevioside and maltodextrin: 1:1 ~ 3:1, then carry out starting to stir and heating up, temperature controls at 65 ~ 70 degree, stir and all dissolve to stevioside for more than 60 minutes, generate intimate mixing liquid;
Adjustment pH value of the present invention, add cyclomaltodextrin glucanotransferase, regulate temperature: it is characterized in that adjusting solution pH value 6.5 ~ 7, add cyclomaltodextrin glucanotransferase, the consumption of cyclomaltodextrin glucanotransferase is that per kilogram stevioside adds 15-40ml, regulate temperature at 65 ~ 70 degree, continue insulation 6 ~ 9 hours;
Deactivation of the present invention, filtration: it is characterized in that solution temperature to be heated to 100 DEG C, continue 10 minutes, be cooled to less than 30 degree, then filter solution after completing;
Absorption of the present invention, washing, alcohol wash: it is characterized in that the solution stream after filtering is through macroporous adsorptive resins, after macroporous adsorptive resins is saturated, rinse with water, water consumption presses 5 ~ 8 times of resin volume in macroporous adsorptive resins, then carry out alcohol wash with 60% ethanol, ethanol consumption presses 1 ~ 1.6 times of resin volume in macroporous adsorptive resins;
Concentrated, spraying dry of the present invention: it is characterized in that the soln using nanofiltration membrane thickening equipment after rinsing concentrates, solution solid content after to be rinsed stops concentrated when reaching 15% ~ 20%, solution after concentrated is proceeded to dealcoholysis in single-effect evaporator, stop when solution solid content reaches 45% ~ 50%, the concentrated solution spraying dry finally will obtained.
Deactivation of the present invention, filtration stage, is characterized in that filtration in deactivation, filtration stage adopts aperture to be that the filter of 1 micron filters.
Concentrated, spray-drying stage of the present invention, is characterized in that concentrating, concentrated condition in spray-drying stage: solution temperature >=85 degree, negative pressure 0.04MPa ~ 0.06 MPa.
The invention has the beneficial effects as follows that the molecular structure of stevioside is modified in the effect by enzyme, reach the object improving stevioside mouthfeel, the stevioside derivative obtained eliminates peculiar smell, obtains high sugariness, low-calorie natural sweeteners, makes product glucoside content reach more than 80%.
Embodiment
Embodiment 1: specific embodiment of the present invention is: the production method step that described general glycoside 80% enzyme improves taste of ribaudiose is:
(1) dissolve: add a certain amount of 10 kg water toward retort, heat up 55 degree, then add 1 kilogram of maltodextrin, preparation maltodextrin solution;
(2) stir: in the maltodextrin solution prepared, add stevioside 1.5 kilograms, start to stir and heat up, temperature controls at 65 degree, stirs and all dissolves to stevioside for 60 minutes, generate intimate mixing liquid;
(3) regulate PH, add cyclomaltodextrin glucanotransferase, regulate temperature: regulate pH value 6.5 to add cyclomaltodextrin glucanotransferase, add 15ml cyclomaltodextrin glucanotransferase, regulate temperature at 65 degree, be incubated 6 hours, make enzyme change after stevioside;
(4) deactivation, filtration: solution is heated to 100 DEG C, keep 10 minutes, after deactivation completes, solution is cooled to less than 30 degree, then filters with the strainer in 1 micron, aperture;
(5) absorption, washing, alcohol wash: get filtered liquid and cross macroporous adsorptive resins, its macroporous resin model is 03FC, the saturated after washing of resin, water consumption carries out flushing extremely without sweet taste by 5 times of resin volume in resin column, then use 60% ethanol elution, ethanol consumption carries out flushing extremely without sweet taste by 1 times of resin volume in resin column;
(6) concentrated, spraying dry: the soln using nanofiltration membrane thickening equipment after wash-out concentrates, concentrated condition: feed temperature >=85 degree, negative pressure 0.04-0.06MP, equipment interlayer pressure < 0.25MP, arrestment when solution solid content after wash-out reaches 45%, then the concentrated solution of acquisition is carried out spraying dry.
Embodiment 2: specific embodiment of the present invention is: the production method step that described general glycoside 80% enzyme improves taste of ribaudiose is:
(1) dissolve: add a certain amount of 10 kg water toward retort, heat up 58 degree, then add 1 kilogram of maltodextrin, preparation maltodextrin solution;
(2) stir: in the maltodextrin solution prepared, add stevioside 1.5 kilograms, start to stir and heat up, temperature controls at 68 degree, stirs and all dissolves to stevioside for 65 minutes, generate intimate mixing liquid;
3) regulate PH, add cyclomaltodextrin glucanotransferase, regulate temperature: regulate pH value 6.8 to add cyclomaltodextrin glucanotransferase, add 30ml cyclomaltodextrin glucanotransferase, regulate temperature at 68 degree, be incubated 7 hours, make enzyme change after stevioside;
(4) deactivation, filtration: solution is heated to 100 DEG C, keep 10 minutes, after deactivation completes, solution is cooled to less than 30 degree, then filters with the strainer in 1 micron, aperture;
(5) absorption, washing, alcohol wash: get filtered liquid and cross macroporous adsorptive resins, its macroporous resin model is 03FC, the saturated after washing of resin, water consumption carries out flushing extremely without sweet taste by 6.5 times of resin volume in resin column, then use 60% ethanol elution, ethanol consumption carries out flushing extremely without sweet taste by 1.3 times of resin volume in resin column;
(6) concentrated, spraying dry: the soln using nanofiltration membrane thickening equipment after wash-out concentrates, concentrated condition: feed temperature >=85 degree, negative pressure 0.04 ~ 0.06MP, equipment interlayer pressure < 0.25MP, concentrated solution is proceeded to single-effect evaporator dealcoholysis, arrestment when solution solid content reaches 48%, then the concentrated solution of acquisition is carried out spraying dry.
Embodiment 3: specific embodiment of the present invention is: the production method step that described general glycoside 80% enzyme improves taste of ribaudiose is:
(1) dissolve: add a certain amount of 10 kg water toward retort, heat up 60 degree, then add 1 kilogram of maltodextrin, preparation maltodextrin solution;
(2) stir: in the maltodextrin solution prepared, add stevioside 1.5 kilograms, start to stir and heat up, temperature controls at 70 degree, stirs and all dissolves to stevioside for 70 minutes, generate intimate mixing liquid;
3) regulate PH, add cyclomaltodextrin glucanotransferase, regulate temperature: regulate pH value 7 to add cyclomaltodextrin glucanotransferase, add 40ml cyclomaltodextrin glucanotransferase, regulate temperature at 70 degree, be incubated 9 hours, make enzyme change after stevioside;
(4) deactivation, filtration: solution is heated to 100 DEG C, keep 10 minutes, after deactivation completes, solution is cooled to less than 30 degree, then filters with the strainer in 1 micron, aperture;
(5) absorption, washing, alcohol wash: get filtered liquid and cross macroporous adsorptive resins, its macroporous resin model is 03FC, the saturated after washing of resin, water consumption carries out flushing extremely without sweet taste by 8 times of resin volume in resin column, then use 60% ethanol elution, ethanol consumption carries out flushing extremely without sweet taste by 1.6 times of resin volume in resin column;
(6) concentrated, spraying dry: the soln using nanofiltration membrane thickening equipment after wash-out concentrates, concentrated condition: feed temperature >=85 degree, negative pressure 0.04-0.06MP, equipment interlayer pressure < 0.25MP, arrestment when solution solid content after wash-out reaches 50%, then the concentrated solution of acquisition is carried out spraying dry.
Claims (1)
1. general glycoside 80% enzyme improves a production method for taste of ribaudiose, and processing step is: dissolve → stirring → adjustment pH value, add cyclomaltodextrin glucanotransferase, adjustment temperature → deactivation, filtration → absorption, washing, alcohol wash → concentrated, spraying dry; It is characterized in that the concrete operations of each processing step are as follows:
Dissolve: in retort, add certain water, be warmed up to 55 ~ 60 degree, add maltodextrin, preparation maltodextrin solution, the ratio of maltodextrin and water selects 1:7 ~ 1:20;
Stir: in maltodextrin solution, add general glycoside 80% stevioside, add the ratio of stevioside and maltodextrin: 1:1 ~ 3:1, then carry out starting to stir and heating up, temperature controls at 65 ~ 70 degree, stir and all dissolve to stevioside for more than 60 minutes, generate intimate mixing liquid;
Regulate pH value, add cyclomaltodextrin glucanotransferase, regulate temperature: adjust solution pH value 6.5 ~ 7, add cyclomaltodextrin glucanotransferase, the consumption of cyclomaltodextrin glucanotransferase is that per kilogram stevioside adds 15-40ml, regulate temperature at 65 ~ 70 degree, continue insulation 6 ~ 9 hours;
Deactivation, filtration: solution temperature is heated to 100 DEG C, continue 10 minutes, be cooled to less than 30 degree, then filter solution after completing;
Absorption, washing, alcohol wash: the solution stream after filtration is through macroporous adsorptive resins, after macroporous adsorptive resins is saturated, rinse with water, water consumption presses 5 ~ 8 times of resin volume in macroporous adsorptive resins, then carry out alcohol wash with 60% ethanol, ethanol consumption presses 1 ~ 1.6 times of resin volume in macroporous adsorptive resins;
Concentrated, spraying dry: the soln using nanofiltration membrane thickening equipment after flushing concentrates, solution solid content after to be rinsed stops concentrated when reaching 15% ~ 20%, solution after concentrated is proceeded to dealcoholysis in single-effect evaporator, stop when solution solid content reaches 45% ~ 50%, the concentrated solution spraying dry finally will obtained;
In described deactivation, filtration process, filtration employing aperture is that the filter of 1 micron filters;
In described concentrated, drying process with atomizing, concentrated condition is: solution temperature >=85 degree, negative pressure 0.04MPa ~ 0.06 MPa.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104894193A (en) * | 2015-05-12 | 2015-09-09 | 曲阜圣仁制药有限公司 | Method for producing total glycosides 95% enzyme modification stevioside |
| CN105146262A (en) * | 2015-06-17 | 2015-12-16 | 黑龙江农垦卉菊海林甜菊糖有限责任公司 | Stevia rebaudiana table sugar and preparation method thereof |
| CN106072462A (en) * | 2016-06-08 | 2016-11-09 | 郑书旺 | The synergism utilizing resin and compound enzyme improves the method for stevioside mouthfeel |
| CN107099567A (en) * | 2017-05-09 | 2017-08-29 | 滁州润海甜叶菊高科有限公司 | A kind of enzyme of general glycoside 95% modifies the production method of stevioside |
| CN107130008A (en) * | 2017-05-18 | 2017-09-05 | 滁州润海甜叶菊高科有限公司 | It is a kind of that the production method that the enzyme of general glycoside 80% modifies stevioside is obtained by organized enzyme deep reaction |
| CN107236773A (en) * | 2017-05-09 | 2017-10-10 | 滁州润海甜叶菊高科有限公司 | It is a kind of that the production method that the enzyme of general glycoside 80% modifies stevioside is obtained by organized enzyme deep reaction |
| CN109293718A (en) * | 2018-10-08 | 2019-02-01 | 山东奥晶生物科技有限公司 | A kind of preparation method of stevia rebaudianum carbohydrase modification stevioside |
| CN109588686A (en) * | 2018-10-31 | 2019-04-09 | 郑书旺 | A kind of composite sweetener and preparation method thereof |
| CN111662941A (en) * | 2020-05-25 | 2020-09-15 | 安徽金禾实业股份有限公司 | Preparation method of glucosyl stevioside |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104894193A (en) * | 2015-05-12 | 2015-09-09 | 曲阜圣仁制药有限公司 | Method for producing total glycosides 95% enzyme modification stevioside |
| CN105146262A (en) * | 2015-06-17 | 2015-12-16 | 黑龙江农垦卉菊海林甜菊糖有限责任公司 | Stevia rebaudiana table sugar and preparation method thereof |
| CN105146262B (en) * | 2015-06-17 | 2018-12-21 | 黑龙江农垦卉菊海林甜菊糖有限责任公司 | A kind of stevia rebaudianum dining table sugar and preparation method thereof |
| CN106072462A (en) * | 2016-06-08 | 2016-11-09 | 郑书旺 | The synergism utilizing resin and compound enzyme improves the method for stevioside mouthfeel |
| CN107099567A (en) * | 2017-05-09 | 2017-08-29 | 滁州润海甜叶菊高科有限公司 | A kind of enzyme of general glycoside 95% modifies the production method of stevioside |
| CN107236773A (en) * | 2017-05-09 | 2017-10-10 | 滁州润海甜叶菊高科有限公司 | It is a kind of that the production method that the enzyme of general glycoside 80% modifies stevioside is obtained by organized enzyme deep reaction |
| CN107130008A (en) * | 2017-05-18 | 2017-09-05 | 滁州润海甜叶菊高科有限公司 | It is a kind of that the production method that the enzyme of general glycoside 80% modifies stevioside is obtained by organized enzyme deep reaction |
| CN109293718A (en) * | 2018-10-08 | 2019-02-01 | 山东奥晶生物科技有限公司 | A kind of preparation method of stevia rebaudianum carbohydrase modification stevioside |
| CN109588686A (en) * | 2018-10-31 | 2019-04-09 | 郑书旺 | A kind of composite sweetener and preparation method thereof |
| CN111662941A (en) * | 2020-05-25 | 2020-09-15 | 安徽金禾实业股份有限公司 | Preparation method of glucosyl stevioside |
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