CN102133254A - Extraction method of dandelion total flavonoids - Google Patents
Extraction method of dandelion total flavonoids Download PDFInfo
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- CN102133254A CN102133254A CN2011100628144A CN201110062814A CN102133254A CN 102133254 A CN102133254 A CN 102133254A CN 2011100628144 A CN2011100628144 A CN 2011100628144A CN 201110062814 A CN201110062814 A CN 201110062814A CN 102133254 A CN102133254 A CN 102133254A
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- herba taraxaci
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Abstract
The invention discloses a method for extracting dandelion total flavonoids by ultrasound, and belongs to the technical field of the extraction of active ingredients of plants. The method comprises the following steps of: crushing dandelion, screening with a 20-mesh screen, adding petroleum ether, uniformly mixing, fully decolorizing, filtering by using filter paper, and evaporating residues for later use; accurately weighing pretreated materials, adding 30 to 50 percent ethanol solution according to a material liquid ratio (g/ml) of 1:(20-30), fully agitating, soaking for 1 hours, extracting with the ultrasonic frequency of 40KHz at the temperature of between 60 and 70 DEG C for 20 to 35 minutes to obtain dandelion total flavonoid extract which is applied to the preparation of anti-inflammatory medicines.
Description
Technical field
The present invention relates to the extracting method technical field of effective ingredients in plant, in particular a kind of method of supersound extraction Herba Taraxaci total flavones.
Background technology
Herba Taraxaci (Taraxacum mongoLicum) belongs to the Compositae herbaceos perennial, it is a kind of important Chinese crude drug, put down in writing according to Compendium of Material Medica: Herba Taraxaci has Detoxicating food-poisoning, effects such as diffusing stagnate gas, heat-transformation poison, swollen, the solid tooth of the evil that disappears, living kidney water, strengthening bone and muscle.Be usually used in treating diseases such as acute mastitis, lymphadenitis, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis and urinary tract infection clinically.Generally believe that at present total flavones is the main effective ingredient of Herba Taraxaci, as if modern pharmacological research shows the Herba Taraxaci total flavones bacillus subtilis and formula paecilomycerol are had good bacteriostasis, and have important antioxidation, can remove ultra-oxygen anion free radical.The chemical research of Herba Taraxaci shows that mainly containing luteolin, Quercetin, luteolin-7-β-D-glucoside, luteolin-4 '-β-D-glucoside, luteolin-7-β-gentiomarin, luteolin-7-β-D-rhamnose glucoside, Quercetin-7-β-D-Fructus Vitis viniferae in the Herba Taraxaci wards off glycosides and different Fructus rhamni (Rhamnus davurica Pall.)-3,7-β-D-Fructus Vitis viniferae is warded off glycosides, isorhamnetin-3-β-flavone compounds such as D-glucoside, has determined that further flavones ingredient is the effective ingredient of Herba Taraxaci.Therefore the extracting method of studying the Herba Taraxaci total flavones will lay the foundation for the further application of Herba Taraxaci.
Summary of the invention
The present invention uses orthogonal design main affecting factors such as solid-liquid ratio, concentration of alcohol, extraction temperature, extraction time is studied, and draws best ultrasonic extracting method.
Technical scheme of the present invention is as follows:
1, a kind of method of supersound extraction Herba Taraxaci total flavones is characterized in that, the Herba Taraxaci herb is pulverized the back cross 20 mesh sieves, adds the petroleum ether mix homogeneously, fully decolours after the filter paper sucking filtration is got filtering residue and volatilized standby; Accurately take by weighing the good material of pretreatment, press the alcoholic solution that solid-liquid ratio (g/mL) 1: 20-30 adds 30-50%, fully shake up the back and soaked 1 hour, under temperature 60-70 ℃, supersonic frequency 40KHz extracts 20-35min, gets the Herba Taraxaci extractive of general flavone, is applied to prepare anti-inflammatory drug.
2, method according to claim 1 is characterized in that, solid-liquid ratio (g/mL) 1: 30, and alcoholic solution concentration 40% is extracted 70 ℃ of temperature, extraction time 30min.
3, method according to claim 1 is characterized in that, the Herba Taraxaci herb is pulverized the back cross 20 mesh sieves, presses solid-liquid ratio (g/mL) 1: 8-10 and adds the petroleum ether mix homogeneously, decolours twice.
The specific embodiment
Now in conjunction with specific embodiments, the invention will be further described, and concrete processing step is as described below:
Embodiment 1
The Herba Taraxaci herb is pulverized the back and is crossed 20 mesh sieves, presses solid-liquid ratio (g/mL) and adds the petroleum ether mix homogeneously at 1: 8, fully after the decolouring, through the filter paper sucking filtration, filtering residue adds solid-liquid ratio (g/mL) again and adds the petroleum ether mix homogeneously at 1: 8, fully after the decolouring, through the filter paper sucking filtration, get filtering residue and volatilize standby; Accurately take by weighing the good material of pretreatment, press the alcoholic solution of solid-liquid ratio (g/mL) adding 40% in 1: 30, fully shaking up the back soaked 1 hour, 70 ℃ of temperature, supersonic frequency 40KHz extracts 30min down, get the Herba Taraxaci extractive of general flavone, detecting the Herba Taraxaci content of total flavone through spectrophotography is 25.3%.
Embodiment 2
The Herba Taraxaci herb is pulverized the back and is crossed 20 mesh sieves, presses solid-liquid ratio (g/mL) and adds the petroleum ether mix homogeneously at 1: 10, fully after the decolouring, through the filter paper sucking filtration, filtering residue adds solid-liquid ratio (g/mL) again and adds the petroleum ether mix homogeneously at 1: 10, fully after the decolouring, through the filter paper sucking filtration, get filtering residue and volatilize standby; Accurately take by weighing the good material of pretreatment, press the alcoholic solution of solid-liquid ratio (g/mL) adding 40% in 1: 20, fully shaking up the back soaked 1 hour, 60 ℃ of temperature, supersonic frequency 40KHz extracts 30min down, get the Herba Taraxaci extractive of general flavone, detecting the Herba Taraxaci content of total flavone through spectrophotography is 21.8%.
Claims (3)
1. the method for a supersound extraction Herba Taraxaci total flavones is characterized in that, the Herba Taraxaci herb is pulverized the back cross 20 mesh sieves, adds the petroleum ether mix homogeneously, fully decolours after the filter paper sucking filtration is got filtering residue and volatilized standby; Accurately take by weighing the good material of pretreatment, press the alcoholic solution that solid-liquid ratio (g/mL) 1: 20-30 adds 30-50%, fully shake up the back and soaked 1 hour, under temperature 60-70 ℃, supersonic frequency 40KHz extracts 20-35min, gets the Herba Taraxaci extractive of general flavone, is applied to prepare anti-inflammatory drug.
2. method according to claim 1 is characterized in that, solid-liquid ratio (g/mL) 1: 30, and alcoholic solution concentration 40% is extracted 70 ℃ of temperature, extraction time 30min.
3. method according to claim 1 is characterized in that, the Herba Taraxaci herb is pulverized the back cross 20 mesh sieves, presses solid-liquid ratio (g/mL) 1: 8-10 and adds the petroleum ether mix homogeneously, decolours twice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100628144A CN102133254A (en) | 2011-03-16 | 2011-03-16 | Extraction method of dandelion total flavonoids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100628144A CN102133254A (en) | 2011-03-16 | 2011-03-16 | Extraction method of dandelion total flavonoids |
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| CN102133254A true CN102133254A (en) | 2011-07-27 |
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| CN2011100628144A Pending CN102133254A (en) | 2011-03-16 | 2011-03-16 | Extraction method of dandelion total flavonoids |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114699438A (en) * | 2022-04-28 | 2022-07-05 | 上海应用技术大学 | Dandelion total flavonoids and extraction method and application thereof |
| CN115252559A (en) * | 2022-07-06 | 2022-11-01 | 沃曼(广东)生物科技有限公司 | Preparation method and application of dandelion total flavone spray-dried powder |
| CN115844938A (en) * | 2022-11-29 | 2023-03-28 | 内蒙古农业大学 | Artemisia sphaerocephala total flavone and preparation method and application thereof |
-
2011
- 2011-03-16 CN CN2011100628144A patent/CN102133254A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114699438A (en) * | 2022-04-28 | 2022-07-05 | 上海应用技术大学 | Dandelion total flavonoids and extraction method and application thereof |
| CN115252559A (en) * | 2022-07-06 | 2022-11-01 | 沃曼(广东)生物科技有限公司 | Preparation method and application of dandelion total flavone spray-dried powder |
| CN115844938A (en) * | 2022-11-29 | 2023-03-28 | 内蒙古农业大学 | Artemisia sphaerocephala total flavone and preparation method and application thereof |
| CN115844938B (en) * | 2022-11-29 | 2024-01-26 | 内蒙古农业大学 | Artemisia sphaerocephala total flavone and preparation method and application thereof |
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Application publication date: 20110727 |