CN102132953B - Method for preparing salvia miltiorrhiza extract microcapsules and use of product thereof - Google Patents
Method for preparing salvia miltiorrhiza extract microcapsules and use of product thereof Download PDFInfo
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- CN102132953B CN102132953B CN 201010619903 CN201010619903A CN102132953B CN 102132953 B CN102132953 B CN 102132953B CN 201010619903 CN201010619903 CN 201010619903 CN 201010619903 A CN201010619903 A CN 201010619903A CN 102132953 B CN102132953 B CN 102132953B
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- miltiorrhiza extract
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Abstract
The invention relates to a method for preparing salvia miltiorrhiza extract microcapsules, which comprises the following steps: dissolving 2.5 to 7.5 grams of salvia miltiorrhiza extract in 10 to 15 milliliters of 95-percet ethanol, and fully stirring to obtain core material solution for later use; and weighing wall material solution in an amount which is 1 to 2 times that of the weight of the core material solution, slowly adding the core material solution into the wall material solution, uniformly stirring, dispersing at 35 to 45 DEG C at a speed of 10,000 to 15,000r/min for 6 to 10 minutes, operating at intervals, stirring at a speed of 1,000 to 2,000r/min at 35 to 45 DEG C for 30 to 40 minutes, and spray drying to obtain the salvia miltiorrhiza extract microcapsules. The microcapsules, when used in cigarette products, can reduce free radical and nuclei aromatic content in the smoke of cigarettes to reduce the damage of smoking to health; the microcapsules have certain health-care effect; meanwhile, the microcapsules have certain fragrance increasing effect, the fragrance of the microcapsules is coordinated with the fragrance of the cigarettes, and the microcapsules do not affect the quality of the cigarettes.
Description
[technical field]
The present invention relates to the preparation method of cigarette additive.More specifically, the present invention relates to a kind of preparation method of salvia miltiorrhiza extract microcapsules, and the salvia miltiorrhiza extract microcapsules that obtains by the method is as the purposes of cigarette additive.
[background technology]
Epidemiology survey shows that smoking can cause human various diseases, such as lung cancer, bronchitis, angiocardiopathy etc.Free radical directly and indirect aggression life macromolecule material and various organelle causes body various infringements of (molecular level, cellular level, histoorgan level) on three kinds of levels, is one of major reason of bringing out various diseases.Experiment showed, that containing free radical in cigarette a bite smog is about 1,000,000 (106) individually, also can excite free radical in vivo indirectly.Condensed-nuclei aromatics in the flue gas is main in the cigarette smoke and the maximum class carcinogen of quantity.Polycyclic Aromatic Hydrocarbons in Cigarette Smoke be formed with a lot of approach, wherein topmost is exactly in the tobacco various organic matter thermal crackings produce the free radical that contains different carbon numbers, participate in forming of condensed-nuclei aromatics by these free radicals.Another main path is to form by the framework structured compound that has existed in the tobacco, such as the steroid compounds.On structure, condensed-nuclei aromatics and the intermediate product in forming process thereof have conjugated unsaturation more.Condensed-nuclei aromatics is that human the discovery the earliest has carcinogenic and material mutagenesis.Therefore the condensed-nuclei aromatics and the radical pair health that reduce in the flue gas are significant.
In order effectively to reduce smoking to the harm of health, domestic researcher adopts according to traditional Chinese medicine and adds the anti-oxidation function that Chinese medicine additive reaches the purpose, particularly traditional Chinese medicine ingredients of harm reduction and health care.The red sage root is the dry root and rhizome of the Labiatae salvia red sage root (Salvia miitiorrhiza Bge.), have stasis-dispelling and pain-killing, activating blood to promote menstruation, the effect of the relieving restlessness that clears away heart-fire, and have good result for the treatment of aspect human cardiovascular and cerebrovascular disease, cancer, anti-ageing the waiting for a long time.Chemical composition mainly is fat-soluble diterpene quinone (Tanshinone I Ia, Tanshinone I and Cryptotanshinone etc.) and water-soluble phenolic acids composition (danshensu, protocatechualdehyde and protocatechuic acid etc.) in the red sage root.Having stronger reproducibility, is good antioxidant.Can eliminate simultaneously the conjugated double bond in condensed-nuclei aromatics and the intermediate product thereof, thereby play the effect that reduces the condensed-nuclei aromatics in the cigarette smoke.Chinese patent application CN101642285 discloses Sodium Danshensu directly to be added on the pipe tobacco and can effectively reduce the DNA oxidative damage that cigarette smoke causes.Chinese patent application CN1511483 discloses tanshinone to be added in the filter tip forming agent and can effectively remove free radical in the cigarette.The extract that Chinese patent application CN1644117, CN101138432, CN1091966 disclose the combination of the red sage root and other Chinese medicines is applied to can reduce harm of smoking on the cigarette.The present invention adopts Salvia root P.E to reduce condensed-nuclei aromatics and the free-radical contents that cigarette burning produces as functional component, to reach the harm reduction health-care effect.
Microcapsules technology is with solid, liquid or the gas wrapping technology in a tiny capsules.Diameter is generally 1～500 μ m.It can make core material and external environment isolated, and the effect that can resist oxygen, light is particularly useful for some easily oxidized materials; Make interactional material mix mutually, coexist; Protect volatile materials, cover the bad smell of some core materials; Make the liquid solid state, be convenient to store and transportation; Have light sensitivity, thermal sensitivity and Li Min and semipermeability, can discharge core material under certain condition, make some specific function goods etc.Microcapsules technology has a wide range of applications in fields such as biological medicines.In technical field of tobacco certain application is arranged also.Chinese patent application CN1457710 adds the oral cavity impression of plant polyphenol microcapsule when improving smoking in cigarette shreds.Chinese patent application CN 1457712 appliable plant polyphenol microcapsules on pipe tobacco to reach the effect of removing interior free yl.Chinese patent application CN1543884 adds polyphenol microcapsules in pipe tobacco can reduce the N-nitroso compounds.
Oxidation-resistant active ingredient is unstable in the Salvia root P.E, very easily being subjected to various environmental conditions to resemble high temperature, humidity, illumination, oxygen etc. affects generation oxidation, polymerization, condensation etc. and loses antioxidation, if it is in preservation, oxidation occurs during transportation and the processing, will greatly reduce its result of use.Can improve the stability of Salvia root P.E after the microencapsulation, prevent it in preservation term and add the oxidation in man-hour and with the interaction of other perfume material.
[summary of the invention]
[problem that invention will solve]
The present invention prevents from it in preservation term and adds oxidation occurs man-hour in order to improve the stability of Salvia root P.E oxidation-resistant active ingredient; Make it be applicable to reduce the infringement that human body is caused because of smoking, particularly performance reduces the effect of free-radical contents and condensed-nuclei aromatics quantity in the flue gas when cigarette burns and sucks, and a kind of preparation method of salvia miltiorrhiza extract microcapsules is provided.
Another object of the present invention provides the purposes of the microcapsules that obtain according to above-mentioned preparation method.
[technical scheme]
In order to realize the foregoing invention purpose, the invention provides a kind of preparation method of salvia miltiorrhiza extract microcapsules, its step is as follows: take by weighing the 2.5-7.5g Salvia root P.E and be dissolved in 10-15mL 95% ethanol, fully stir and obtain the core solution for standby; Take by weighing the wall material solution doubly in described core solution weight 1-2, described core solution is slowly added in the wall material solution, stir and disperse 6-10min under rear 35-45 ℃, 10000-15000r/min, intermittently operated, then at 1000-2000r/min, the 35-45 ℃ of lower 30-40min that stirs, obtain described salvia miltiorrhiza extract microcapsules after the spray-drying.
In kind of the present invention, wall material solution can be selected various compositions and the preparation method that usually adopts in the microcapsules preparation field.A kind ofly preferred embodiment be: take by weighing 0.5～1 part of soybean protein isolate, 1～2 part of maltodextrin, 0～0.5 part in gelatin in weight portion, at room temperature gelatin is joined in 7.15-10 part water, under 60 ℃, stir 25-35min after mixing, then slowly add soybean protein isolate and bud dextrin, again at 60 ℃ of lower 25-35min that stir, naturally obtain described wall material solution after the cooling after mixing.
In the present invention, preferably, described core solution is added when disperseing behind the wall material solution, described intermittently operated can be every dispersion 2min, leaves standstill 3min, repetitive operation 3-5 time.
A kind of preferred specific embodiment of preparation in accordance with the present invention takes by weighing the 2.5-7.5g Salvia root P.E and is dissolved in 10-15mL 95% ethanol, fully stirs and obtains the core solution for standby; Take by weighing the wall material solution doubly in described core solution weight 1-2, described core solution is slowly added in the wall material solution, stir and disperse 8min under rear 40 ℃, 12000r/min, intermittently operated, then at 2000r/min, 40 ℃ of lower 30min that stir, obtain described salvia miltiorrhiza extract microcapsules after the spray-drying.
In the present invention, spray-drying is the routine operation of this area, preferably, can carry out under 90 ℃ of 190 ℃ of EATs, leaving air temp.
The present invention also provides salvia miltiorrhiza extract microcapsules that the above-mentioned preparation method that can correct one's mistakes the obtains purposes as cigarette additive.
Especially, described salvia miltiorrhiza extract microcapsules is in tobacco leaf, thin slice or stem gross weight 0.2 ‰～5 ‰ as the consumption of cigarette additive, particularly preferably 0.5 ‰～1 ‰.
In the present invention, Salvia root P.E is the product that market is bought, for example Salvia root P.E of Zhengzhou litchi promise bio tech ltd production and sales.Salvia root P.E is that the dry root and rhizome extraction of labiate red sage root Salviamiltiorrhiza Bge. processes, be the brownish red pulvis, main component comprises fat-soluble non-quinochrome compounds, such as tanshinone IIA, Tanshinone II B, Cryptotanshinone and isomers thereof, and soluble salvianolic acid constituents such as protocatechualdehyde, danshensu.Wherein, Cryptotanshinone is antibiotic active ingredient.
[beneficial effect]
The present invention effectively carries out Salvia root P.E microencapsulation and is successfully applied in the cigarette product, and the effect that reaches is: reduce free radical and condensed-nuclei aromatics quantity in the cigarette smoke, reduce because of the infringement of smoking to health; Microencapsulation makes Salvia root P.E avoid extraneous undesirable element, and such as the impact of light, oxygen, temperature, humidity, pH etc., the defencive function composition improves product stability; Microencapsulation makes liquid curing, is convenient to preservation, transportation and use; Avoid the interaction between technique causes in the Cigarette processing process the spices; Has certain health-care effect; Have simultaneously certain flavouring effect, and fragrance and cigarette are fragrant coordinates, the quality of cigarette itself is not had harmful effect.
[specific embodiment]
Following non-limiting example is used for illustrating in greater detail the present invention.
Embodiment 1
Take by weighing the 7.5g Salvia root P.E and be dissolved in 15mL 95% ethanol, fully stir rear as core solution.Take by weighing the 3.5g gelatin and add in the 70mL water, be heated to 60 ℃ and stir 30min, add the 14g maltodextrin again, the 7g soybean protein isolate stirs 30min.Be cooled to room temperature and obtain wall material solution.Core solution is slowly added in the wall material mixed liquor, at 40 ℃, disperse 8min (disperse 2min, intermittently 3min repeats 4 times at every turn) under the 12000r/min after stirring is mixed.Then 2000r/min, 40 ℃, both stir behind the 30min to get microcapsule solution, in 90 ℃ of lower spray-dryings of 190 ℃ of leaving air temps of EAT, both salvia miltiorrhiza extract microcapsules goods (A).
Embodiment 2
Taking by weighing the 2.5g Salvia root P.E is dissolved in 10mL 95% ethanol, as core.At 60 ℃, add the 3.5g soybean protein isolate in the 50mL water, the 7g maltodextrin stirs 30min.Be cooled to room temperature.Core solution is slowly added, after stirring is mixed, at 35 ℃, disperse 6min (disperse 2min, intermittently 3min repeats 3 times) under the 15000r/min.Then 1000r/min, 35 ℃, both stir behind the 40min to get microcapsule solution, in 90 ℃ of lower spray-dryings of 190 ℃ of leaving air temps of EAT, both salvia miltiorrhiza extract microcapsules goods (B).
Embodiment 3
Taking by weighing the 5g Salvia root P.E is dissolved in 10mL 95% ethanol and makes core solution.The 1g gelatin is added in the 70mL water, be added to 60 ℃ and stir 30nin, add soybean protein isolate 2g again, maltodextrin 4g stirs 30min, is cooled to room temperature and obtains wall material solution.Core solution is slowly added in the wall material mixed liquor, at 40 ℃, disperse 10min (disperse 2min, intermittently 3min repeats 5 times) under the 10000r/min after stirring is mixed.Then 2000r/min, 30 ℃, both stir behind the 40min to get microcapsule solution, in 90 ℃ of lower spray-dryings of 190 ℃ of leaving air temps of EAT, both salvia miltiorrhiza extract microcapsules goods (C).
Embodiment 4
Salvia miltiorrhiza extract microcapsules goods (A), (B), (C) are added in the brand cigarette leaf group of Yunnan in 0.5 ‰ and 1 ‰ ratio respectively, roll into cigarette.Press ISO4387 standard method smoking.Measure flue gas free radical and condensed-nuclei aromatics.
Determining free radicals adopts home-made contrivance to capture free radical, and the electron spin EPR spectrometer is measured.Condensed-nuclei aromatics adopts the makings chromatographic process to measure.Data see Table 1 and table 2.
Table 1 salvia miltiorrhiza extract microcapsules is on the impact of flue gas free radical
Table 2 salvia miltiorrhiza extract microcapsules is on the impact of condensed-nuclei aromatics
The result shows that this salvia miltiorrhiza extract microcapsules can reduce free radical and condensed-nuclei aromatics quantity in the cigarette smoke at cigarette product, therefore reduces because of the infringement of smoking to health; Has certain health-care effect; Have simultaneously certain flavouring effect, and fragrance and cigarette are fragrant coordinates, the quality of cigarette itself is not had harmful effect.
Claims (5)
1. the preparation method of a salvia miltiorrhiza extract microcapsules is characterized in that step is as follows: take by weighing the 2.5-7.5g Salvia root P.E and be dissolved in 10-15mL 95% ethanol, fully stir and obtain the core solution for standby; Take by weighing the wall material solution doubly in described core solution weight 1-2, described core solution is slowly added in the wall material solution, stir and disperse 6-10min under rear 35-45 ℃, 10000-15000r/min, intermittently operated, then at 1000-2000r/min, the 35-45 ℃ of lower 30-40min that stirs, obtain described salvia miltiorrhiza extract microcapsules after the spray-drying; Described intermittently operated is every dispersion 2min, leaves standstill 3min, repetitive operation 3-5 time; Described spray-drying is carried out under 90 ℃ of 190 ℃ of EATs, leaving air temp;
The preparation process of described wall material solution is: take by weighing 0.5～1 part of soybean protein isolate, 1～2 part of maltodextrin, 0～0.5 part in gelatin in weight portion, at room temperature gelatin is joined in 7.15-10 part water, under 60 ℃, stir 25-35min after mixing, then slowly add soybean protein isolate and bud dextrin, again at 60 ℃ of lower 25-35min that stir, naturally obtain described wall material solution after the cooling after mixing.
2. the preparation method of salvia miltiorrhiza extract microcapsules according to claim 1, it is characterized in that stirring and disperse 8min under rear 40 ℃, 12000r/min, then intermittently operated at 2000r/min, 40 ℃ of lower 30min that stir, obtains described salvia miltiorrhiza extract microcapsules after the spray-drying.
3. the salvia miltiorrhiza extract microcapsules that obtains among the preparation method claimed in claim 1 is as the purposes of cigarette additive.
4. purposes according to claim 3, the consumption that it is characterized in that described salvia miltiorrhiza extract microcapsules is in tobacco leaf, thin slice or stem gross weight 0.2 ‰～5 ‰.
5. purposes according to claim 3, the consumption that it is characterized in that described salvia miltiorrhiza extract microcapsules is in tobacco leaf, thin slice or stem gross weight 0.5 ‰～1 ‰.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010619903 CN102132953B (en) | 2010-12-29 | 2010-12-29 | Method for preparing salvia miltiorrhiza extract microcapsules and use of product thereof |
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| CN 201010619903 CN102132953B (en) | 2010-12-29 | 2010-12-29 | Method for preparing salvia miltiorrhiza extract microcapsules and use of product thereof |
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| CN102132953B true CN102132953B (en) | 2013-04-03 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102578702A (en) * | 2011-12-30 | 2012-07-18 | 华宝食用香精香料（上海）有限公司 | Hydroxypropyl-beta-cyclodextrin clathrate preparation method of cream for cigarette |
| CN102920013B (en) * | 2012-11-19 | 2015-02-11 | 湖南中烟工业有限责任公司 | Tobacco extractive microcapsule and preparation method thereof |
| CN102920014B (en) * | 2012-11-19 | 2015-04-22 | 湖南中烟工业有限责任公司 | Buccal type smoke-free tobacco product |
| CN105919154A (en) * | 2016-06-28 | 2016-09-07 | 云南瑞升烟草技术（集团）有限公司 | Blasting bead filter rod for reducing free radical in cigarette smoke |
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| US5746231A (en) * | 1993-01-11 | 1998-05-05 | Craig Lesser | Tobacco smoke filter for removing toxic compounds |
| CA2239494C (en) * | 1995-12-19 | 2000-08-22 | Craig Lesser | Cigarette filter containing microcapsules |
| CN1170547C (en) * | 2000-09-29 | 2004-10-13 | 国家海洋药物工程技术研究中心 | No-stinking fish-oil composite micro-capsule and preparation process |
| CN100427083C (en) * | 2005-11-16 | 2008-10-22 | 江南大学 | Method for preparing flavone aglycone micro capsule |
| CN101708451B (en) * | 2009-12-25 | 2011-11-09 | 陕西科技大学 | Method for preparing high-temperature resistant essence microcapsules |
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Address after: 850000 Lhasa economic and Technological Development Zone, Tibet autonomous region, road, investment building, Patentee after: HUABAO FLAVOURS & FRAGRANCES CO.,LTD. Address before: 850000 Lhasa economic and Technological Development Zone, Tibet autonomous region, road, investment building, Patentee before: Huabao flavor Ltd. |
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Address after: 850000 Lhasa economic and Technological Development Zone, Tibet autonomous region, road, investment building, Patentee after: Huabao flavor Ltd. Address before: 201821 Yecheng Road, Shanghai, No. 1299, No. Patentee before: Huabao Flavours & Fragrances Co.,Ltd |
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Effective date of registration: 20170406 Address after: 201821 Road, Jiading Industrial Zone, Shanghai, building C, No. 1299 Patentee after: SHANGHAI HUABAO PEACOCK ESSENCE CO.,LTD. Address before: 850000 Lhasa economic and Technological Development Zone, Tibet autonomous region, road, investment building, Patentee before: HUABAO FLAVOURS & FRAGRANCES CO.,LTD. |
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