CN102114061B - Method for extracting polyphenols from Siberian nitraria peels - Google Patents
Method for extracting polyphenols from Siberian nitraria peels Download PDFInfo
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Abstract
The invention relates to biotechnology, particularly relates to a method for extracting polyphenols from Siberian nitraria peels. The method comprises the following steps: (1) cleaning; (2) degreasing; (3) performing microwave-assisted extraction; (4) performing ultrasonic extraction; (5) removing impurities; (6) testing by determining contents of polyphenols (counted with content of proanthocyanidin) using sulfuric acid-vanillin method, hydrochloric acid-vanillin method and so on. The invention provides a set of sound technical scheme for extracting polyphenols from Siberian nitraria peels. Compared with the conventional extraction method, the method provided by the invention has the advantages of high extraction rate, high safety and simple operation, increases added value of product, and to a great extent, provides a prerequisite for large-scale processing of Siberian nitraria peels.
Description
Technical field
The present invention relates to biotechnology, specifically relate to a kind of method of from the Cortex Acanthopanacis Radicis peel, extracting polyphenols.
Background technology
Polyphenols is meant the general designation of chemical element in one group of plant, gains the name because of having a plurality of phenolic group group.Polyphenols has very strong antioxidation, and procyanidin wherein (OPC) is the efficient antioxidants of finding so far of plant origin.France academy of science had once done polyphenols and had removed the free radical activity experiment, and the result thinks the strongest effectively one of free radical scavenger that wherein procyanidin (OPC) is up to now to be found.Its antioxidant activity is ascorbic 20 times, 50 times of vitamin E.Polyphenols has protection cardiovascular and prophylaxis of hypertension effect, antioxidation and antienzyme effect; Cholesterol reducing and low-density lipoprotein white level are arranged, and prevention thrombosis helps the effect that prevents cardiovascular and cerebrovascular disease to take place; Have the protection cell DNA to exempt from the oxidative damage of free radical, prevention causes the effect of the gene mutation, radioprotective of cancer etc.In countries such as America and Europes; Polyphenols is enjoyed good reputations such as " skin vitamin ", " oral cosmetics ", is the crease-resistant product of a kind of beauty treatment that quite receives Mrs all ages favor, can stop the appearance of wrinkle of skin and vesicle; Keep the submissive smooth of skin; Effectively prophylactic treatment spontaneous or by drug induced gastric and duodenal ulcers, improve flexibility of joint, repair the collagen protein in the connective tissue.
The wild fruit of Cortex Acanthopanacis Radicis is called as " the 3rd type of fruit ", has huge economic utilization potential value.The Cortex Acanthopanacis Radicis resource is abundant in China's output, distributes extensively, and concentrates distribution relatively in the area, Qaidam in Qinghai, and condition of utilization is superior.The Cortex Acanthopanacis Radicis resource that Qaidam area distributes is 150 ~ 2,000,000 mu, concentrates relatively to distribute and have about 500,000 mu of a Cortex Acanthopanacis Radicis woods that exploitation is worth, and peel 1.5 ~ 2.5 ten thousand ton can be produced in annual institute Fructus Nitraria schoberi actual quantities 6 ~ 100,000 of tying ton, and stock number is very abundant, demands urgently developing.According to research; Contain (18 kinds more than in abundant carotene, carotenoid, vitamin, binary and ternary hydroxy acid, aminoacid in berry of nitraria tangutorum bobr, fruit juice, peel and the pit; Wherein 8 kinds of essential amino acids content are abundant), pectin, Pectin, proto, flavone, anthocyanin, spend glycoside, polysaccharide, terpenoid, peptide, steroidal class, alkaloid in vain and abundant fruit acid, protein, arnotto plain, various trace elements etc.The Cortex Acanthopanacis Radicis polyphenols that extracts through biotechnology as raw material with the Cortex Acanthopanacis Radicis peel; Have outside the characteristics of similar other polyphenols, form, kept the active component in the peel because of the peel that utilizes natural wild acinus extracts; Have health care simultaneously, added value is high.Conventional method of extraction is soxhlet extraction, hot water extraction and organic solvent circumfluence method etc., but can only extract pigment merely or produce low value-added product with the Cortex Acanthopanacis Radicis peel.
Summary of the invention
The technical problem that the present invention will solve is to the deficiency that exists in the prior art, and a kind of method of from the Cortex Acanthopanacis Radicis peel, extracting polyphenols is provided.
A kind of method of from the Cortex Acanthopanacis Radicis peel, extracting polyphenols of the present invention is achieved through following technical proposals: a kind of method of from the Cortex Acanthopanacis Radicis peel, extracting polyphenols of the present invention comprises the steps:
(1) cleans: be meant that the Cortex Acanthopanacis Radicis peel cleans to remove the process of silt, polysaccharide and water-solubility impurity with circulating water;
(2) defat: be meant and want the dialogue burbark to carry out the process of ungrease treatment removal lipid material before extracting, adopt organic solvent degreasings such as ether, petroleum ether;
(3) microwave-assisted extracts: be meant the process of extracting polyphenols through microwave-assisted, and the Cortex Acanthopanacis Radicis peel oven drying at low temperature that defat is intact, in the 2450MHZ microwave frequency, under the microwave power of 100W～1000W, assisted extraction 1～100 second;
(4) ultrasonic extraction: be meant the process through the supersound extraction polyphenols, the Cortex Acanthopanacis Radicis peel that extracts through microwave-assisted is under 25～100KHZ in supersonic frequency, extracts 1～100 minute, and extracting temperature is 10～90 ℃;
(5) remove impurity: the polyphenols study of extraction contains protein, polysaccharide and other impurity, adopts the high concentration alcohol deposition method to remove methods such as polysaccharide, neutral salt saturation removal protein and removes impurity;
(6) detect: detect the content of polyphenols in the content of procyanidin, detection method adopts methods such as sulphuric acid-vanillin method, hydrochloric acid-vanillin to measure its content.
A kind of method of from the Cortex Acanthopanacis Radicis peel, extracting polyphenols of the present invention has following beneficial effect compared with prior art: a kind of method of from the Cortex Acanthopanacis Radicis peel, extracting polyphenols comprises steps such as microwave-assisted extraction, ultrasonic extraction and remove impurity; For the intensive processing of Cortex Acanthopanacis Radicis peel, comprehensive utilization provide a cover perfect technical scheme, broken through the present situation of extracting pigment merely or producing low value-added product with the Cortex Acanthopanacis Radicis peel.The content that adopts the polyphenols (in the content of procyanidin) that this method extracts has been brought up to 11.58mg/g by the 3.21mg/g of traditional method for extracting, has improved extraction of polyphenol substance rate in the Cortex Acanthopanacis Radicis peel greatly.And have extraction efficiency height, characteristics such as safe and simple.
This invention provides a cover perfect technical scheme for from the Cortex Acanthopanacis Radicis peel, extracting polyphenols; More traditional extracting mode has advantages such as extraction ratio is high, safe and simple, raising added value of product, and the scale of dialogue burbark processing provides prerequisite to a great extent.
Description of drawings
Fig. 1 is a kind of method process chart that from the Cortex Acanthopanacis Radicis peel, extracts polyphenols of the present invention.
The specific embodiment
Below in conjunction with accompanying drawing and embodiment a kind of method and technology scheme of from the Cortex Acanthopanacis Radicis peel, extracting polyphenols of the present invention is further described.
As shown in Figure 1, a kind of method of from the Cortex Acanthopanacis Radicis peel, extracting polyphenols of the present invention comprises the steps:
(1) cleans: be meant that the Cortex Acanthopanacis Radicis peel cleans to remove the process of silt, polysaccharide and water-solubility impurity with circulating water;
(2) defat: be meant and want the dialogue burbark to carry out the process of ungrease treatment removal lipid material before extracting, adopt organic solvent degreasings such as ether, petroleum ether;
(3) microwave-assisted extracts: be meant the process of extracting polyphenols through microwave-assisted, and the Cortex Acanthopanacis Radicis peel oven drying at low temperature that defat is intact, in the 2450MHZ microwave frequency, under the microwave power of 100W～1000W, assisted extraction 1～100 second;
(4) ultrasonic extraction: be meant the process through the supersound extraction polyphenols, the Cortex Acanthopanacis Radicis peel that extracts through microwave-assisted is under 25～100KHZ in supersonic frequency, extracts 1～100 minute, and extracting temperature is 10～90 ℃;
(5) remove impurity: the polyphenols study of extraction contains protein, polysaccharide and other impurity, adopts the high concentration alcohol deposition method to remove methods such as polysaccharide, neutral salt saturation removal protein and removes impurity;
(6) detect: detect the content of polyphenols in the content of procyanidin, detection method adopts methods such as sulphuric acid-vanillin method, hydrochloric acid-vanillin to measure its content.
Embodiment 1.
A kind of method of from the Cortex Acanthopanacis Radicis peel, extracting polyphenols of the present invention comprises the steps:
(1) clean: the Cortex Acanthopanacis Radicis peel cleans to remove silt, polysaccharide and water-solubility impurity with circulating water;
(2) defat: because the Cortex Acanthopanacis Radicis peel contains certain lipid material, influence the extraction of polyphenol substance effect, want the dialogue burbark to carry out ungrease treatment before therefore extracting, remove lipid material.This method adopts organic solvent degreasings such as ether, petroleum ether;
(3) microwave-assisted extracts: the Cortex Acanthopanacis Radicis peel oven drying at low temperature that defat is intact, and in the 2450MHZ microwave frequency, under the microwave power of 100W, assisted extraction 100 seconds;
(4) ultrasonic extraction: the Cortex Acanthopanacis Radicis peel that extracts through microwave-assisted is under the 25KHZ in supersonic frequency, extracts 100 minutes, and extracting temperature is 90 ℃;
(5) remove impurity: the polyphenols study of extraction contains protein, polysaccharide and other impurity, needs to adopt certain method to remove impurity, in order to avoid influence extraction ratio; This method adopts the high concentration alcohol deposition method to remove methods such as polysaccharide, neutral salt saturation removal protein and removes impurity;
(6) detect: detect the content of polyphenols in the content of procyanidin, detection method adopts methods such as sulphuric acid-vanillin method, hydrochloric acid-vanillin to measure the content of procyanidins.
Embodiment 2.
A kind of method of from the Cortex Acanthopanacis Radicis peel, extracting polyphenols of the present invention comprises the steps:
(1) clean: the Cortex Acanthopanacis Radicis peel cleans to remove silt, polysaccharide and water-solubility impurity with circulating water;
(2) defat: because the Cortex Acanthopanacis Radicis peel contains certain lipid material, influence the extraction of polyphenol substance effect, want the dialogue burbark to carry out ungrease treatment before therefore extracting, remove lipid material.This method adopts organic solvent degreasings such as ether, petroleum ether;
(3) microwave-assisted extracts: the Cortex Acanthopanacis Radicis peel oven drying at low temperature that defat is intact, and in the 2450MHZ microwave frequency, under the microwave power of 500W, assisted extraction 80 seconds;
(4) ultrasonic extraction: the Cortex Acanthopanacis Radicis peel that extracts through microwave-assisted is under the 50KHZ in supersonic frequency, extracts 80 minutes, and extracting temperature is 70 ℃;
(5) remove impurity: the polyphenols study of extraction contains protein, polysaccharide and other impurity, needs to adopt certain method to remove impurity, in order to avoid influence extraction ratio; This method adopts the high concentration alcohol deposition method to remove methods such as polysaccharide, neutral salt saturation removal protein and removes impurity;
(6) detect: detect the content of polyphenols in the content of procyanidin, detection method adopts methods such as sulphuric acid-vanillin method, hydrochloric acid-vanillin to measure the content of procyanidins.
Embodiment 3.
A kind of method of from the Cortex Acanthopanacis Radicis peel, extracting polyphenols of the present invention comprises the steps:
(1) clean: the Cortex Acanthopanacis Radicis peel cleans to remove silt, polysaccharide and water-solubility impurity with circulating water;
(2) defat: because the Cortex Acanthopanacis Radicis peel contains certain lipid material, influence the extraction of polyphenol substance effect, want the dialogue burbark to carry out ungrease treatment before therefore extracting, remove lipid material.This method adopts organic solvent degreasings such as ether, petroleum ether;
(3) microwave-assisted extracts: the Cortex Acanthopanacis Radicis peel oven drying at low temperature that defat is intact, and in the 2450MHZ microwave frequency, under the microwave power of 800W, assisted extraction 50 seconds;
(4) ultrasonic extraction: the Cortex Acanthopanacis Radicis peel that extracts through microwave-assisted is under the 80KHZ in supersonic frequency, extracts 20 minutes, and extracting temperature is 40 ℃;
(5) remove impurity: the polyphenols study of extraction contains protein, polysaccharide and other impurity, needs to adopt certain method to remove impurity, in order to avoid influence extraction ratio; This method adopts the high concentration alcohol deposition method to remove methods such as polysaccharide, neutral salt saturation removal protein and removes impurity;
(6) detect: detect the content of polyphenols in the content of procyanidin, detection method adopts methods such as sulphuric acid-vanillin method, hydrochloric acid-vanillin to measure the content of procyanidins.
Embodiment 4.
A kind of method of from the Cortex Acanthopanacis Radicis peel, extracting polyphenols of the present invention comprises the steps:
(1) clean: the Cortex Acanthopanacis Radicis peel cleans to remove silt, polysaccharide and water-solubility impurity with circulating water;
(2) defat: because the Cortex Acanthopanacis Radicis peel contains certain lipid material, influence the extraction of polyphenol substance effect, want the dialogue burbark to carry out ungrease treatment before therefore extracting, remove lipid material.This method adopts organic solvent degreasings such as ether, petroleum ether;
(3) microwave-assisted extracts: the Cortex Acanthopanacis Radicis peel oven drying at low temperature that defat is intact, and in the 2450MHZ microwave frequency, under the microwave power of 1000W, assisted extraction 10 seconds;
(4) ultrasonic extraction: the Cortex Acanthopanacis Radicis peel that extracts through microwave-assisted is under the 100KHZ in supersonic frequency, extracts 10 minutes, and extracting temperature is 10 ℃;
(5) remove impurity: the polyphenols study of extraction contains protein, polysaccharide and other impurity, needs to adopt certain method to remove impurity, in order to avoid influence extraction ratio; This method adopts the high concentration alcohol deposition method to remove methods such as polysaccharide, neutral salt saturation removal protein and removes impurity;
(6) detect: detect the content of polyphenols in the content of procyanidin, detection method adopts methods such as sulphuric acid-vanillin method, hydrochloric acid-vanillin to measure the content of polyphenols.
Claims (1)
1. method of from the Cortex Acanthopanacis Radicis peel, extracting polyphenols, it is characterized in that: described method comprises the steps:
(1) cleans: be meant that the Cortex Acanthopanacis Radicis peel cleans to remove the process of silt, polysaccharide and water-solubility impurity with circulating water;
(2) defat: be meant and want the dialogue burbark to carry out the process of ungrease treatment removal lipid material before extracting, adopt ether, petroleum ether organic solvent degreasing;
(3) microwave-assisted extracts: be meant the process of extracting polyphenols through microwave-assisted, and the Cortex Acanthopanacis Radicis peel oven drying at low temperature that defat is intact, in the 2450MHZ microwave frequency, under the microwave power of 100W～1000W, assisted extraction 1～100 second;
(4) ultrasonic extraction: be meant the process through the supersound extraction polyphenols, the Cortex Acanthopanacis Radicis peel that extracts through microwave-assisted is under 25～100KHZ in supersonic frequency, extracts 1～100 minute, and extracting temperature is 10～90 ℃;
(5) remove impurity: the polyphenols study of extraction contains protein, polysaccharide and other impurity, adopts high concentration alcohol deposition method removal polysaccharide, neutral salt saturation to remove the protein method and remove impurity;
(6) detect: detect the content of polyphenols in the content of procyanidin, detection method adopts sulphuric acid-vanillin method, hydrochloric acid-vanillin method to measure its content.
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| CN105077215B (en) * | 2015-08-20 | 2017-05-10 | 中国科学院西北高原生物研究所 | Cordyceps sinensis freshness-retaining product taking Nitraria tangutorun polysaccharide as natural bacteriostatic agent |
| CN105343180B (en) * | 2015-11-13 | 2020-12-01 | 潍坊医学院 | Extraction method of cedrela sinensis seed polyphenol and application of cedrela sinensis seed polyphenol in treating Parkinson's disease |
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