CN102113521A - Plant virus-resisting preparation and applications thereof - Google Patents
Plant virus-resisting preparation and applications thereof Download PDFInfo
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- CN102113521A CN102113521A CN2010105749329A CN201010574932A CN102113521A CN 102113521 A CN102113521 A CN 102113521A CN 2010105749329 A CN2010105749329 A CN 2010105749329A CN 201010574932 A CN201010574932 A CN 201010574932A CN 102113521 A CN102113521 A CN 102113521A
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Abstract
The invention discloses a plant virus-resisting preparation which comprises the following components: Pseudomonas fluorescens CZ (CGMCC No. 4118) culture solution and Tween-20, wherein in the Pseudomonas fluorescens CZ culture, the viable count of Pseudomonas fluorescens CZ is 2*10<10> spores/mL, and the mass of Tween-20 is 0-5% of the total mass of the preparation; and Pseudomonas fluorescens CZ has already been collected in China General Microbiological Culture Collection Center (CGMCC) on August 23, 2010. The plant virus-resisting preparation discloses by the invention can be used for resisting plant viruses, killing viruses on operation tools, and promoting crop growth. The plant virus-resisting preparation has the effects of inactivating virus particles, inhibiting virus replication and proliferation, and inducing host resistance; and the preventive effect is over 80%.
Description
Technical field
The present invention relates to a kind of Antiphytoviral preparation and application thereof.
Background technology
Virus disease is one of important disease of crops.At present, the control efficiency of anti-plant virus agent or safety are also undesirable, can not satisfy the demand of agricultural far away, are badly in need of the development of new anti-virus formulation.Although chemical pesticide still accounts for main body now, consider that from the angle of sustainable development it is the mission critical of realizing in the industrialization biological control that development can produce efficient, highly active biogenic antivirotic.
China's document: " mechanism of action pre-test of a kind of bacterioprotein CZ control tobacco mosaic virus (TMV) "; Wu Huihui; Wang Fenglong, Shen Lili, plant protection; the 34th volume the 3rd phase (2008); the 74-77 page or leaf has put down in writing a kind of pseudomonas fluorescens CZ and tobacco mosaic virus (TMV) is had refused anti-effect, but it only is mechanism research; be not made into the preparation that is applicable to suitability for industrialized production and application, there is a difficult problem in its practical application.
Summary of the invention
At above-mentioned prior art, the invention provides a kind of Antiphytoviral preparation, and use.
The present invention is achieved by the following technical solutions:
A kind of Antiphytoviral preparation, it is composed of the following components: pseudomonas fluorescens CZ culture fluid, polysorbas20, wherein, in the pseudomonas fluorescens CZ culture fluid, the viable count of pseudomonas fluorescens CZ is 20,000,000,000 spore/milliliters, and the quality of polysorbas20 accounts for 0～5% (when the quality of polysorbas20 is 0%, that is: do not contain polysorbas20) of preparation gross mass; Described pseudomonas fluorescens CZ called after Pseudomonas fluorescens was preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on 08 23rd, 2010, and its deposit number is CGMCCNO.4118.
Preferably, the quality of described polysorbas20 accounts for 1～5% of preparation gross mass, and most preferred is 3%.
Described Antiphytoviral preparation adds polysorbas20 in the pseudomonas fluorescens CZ culture fluid in the preparation, and mixing gets final product.
Described Antiphytoviral preparation can be used in the TMV virus disease of the treatment viroses of plant, particularly tobacco.
Described Antiphytoviral preparation can also carry out dissipation to the virus on the manipulate tools, reduces disease spread.
Described Antiphytoviral preparation can also promote the growth of crop, especially tobacco.
Pseudomonas fluorescens CZ involved in the present invention, its cultural method, biological nature, active substance etc., open, be documented in Chinese document: " optimal conditions of fermentation of antagonism TMV bacterium CZ and the Primary Study of active substance thereof ", Wu Huihui, Wang Fenglong, Chen Dexin, the Chinese tobacco science, 2009,30 (1): the 50-53 page or leaf.Therefore, do not repeat them here.
Of the present invention and Antiphytoviral preparation has the passivation virion, suppresses the ability that host resistance is bred and induced to virus replication; Preventive effect is more than 80%.Aspect crop (tobacco is main) virus disease control, has very high using value.Though pseudomonas fluorescens CZ can resist the characteristic of TMV open, but the present invention is prepared into the preparation that is suitable for industrial applications with it, in the prescription, pseudomonas fluorescens CZ and polysorbas20 all have inhibitory action to TMV, but but and polysorbas20 can make non-cultivation conditions bacterium return to cultivation conditions, increase the quantity of bacterium, can play collaborative effect.The consumption of preferred the best that the consumption of polysorbas20 draws behind a large amount of experiment screenings for the inventor, when the quality of polysorbas20 accounts for preparation gross mass 3%, its best results.
Description of drawings
Pseudomonas fluorescens CZ called after Pseudomonas fluorescens of the present invention, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on 08 23rd, 2010, its deposit number is CGMCCNO.4118, the preservation address is: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology of the Chinese Academy of Sciences, postcode: 100101.
Fig. 1 be among the embodiment 1 CZ to the mode of action schematic diagram of TMV.
Embodiment
The present invention is further illustrated below in conjunction with embodiment.
Embodiment 1CZ preparation is to the effect of TMV
Get 5-6 leaf three lives phase NN cigarette, do following processing: as shown in Figure 1.
1 (inducing): 24h before the inoculation sprays CZ, NB, water respectively, every strain 10ml, inoculation TMV+ water.
2 (passivation): CZ, NB, water mix back 15min inoculation respectively with TMV.
3 (inhibition): inoculation TMV+ water behind the 24h, sprays CZ, NB, water respectively, every strain 10ml.
Inoculation back 3-5 days, statistics is respectively handled withered spot number, calculates withered spot inhibiting rate.The result is as shown in table 1.
Table 1CZ measures (preventive effect %) to the mode of action of TMV
Find out that by table 1 CZ is to the mode of action of TMV, and is the most remarkable with passivation, preventive effect is 99.08%, and inducing action is taken second place, preventive effect 95.39%; The inhibitory action preventive effect is minimum also 88.42%.
Embodiment 2CZ preparation is to the disinfective action of scissors
The scissors of sterilization (the sick leaf of 1g grinds, and adds the 80ml sterile water) in 80 times TMV juice soaks 30s, soaks 10s respectively in CZ, NB, water after the taking-up, on common cigarette NC89 of 5-6 leaf phase, cuts 3 leaves in top.Each handles 9 strains, and 3 repetitions are whenever cut a strain and soaked once viral juice and treatment fluid, 15 days " Invest, Then Investigate " disease indexs, and the result is as shown in table 2.
Table 2CZ preparation is to the disinfective action of scissors
Find out that by table 2 the CZ preparation all has disinfective action to scissors, disease index is respectively 0.41, all is lower than sterile water and handles 8.64; The incidence of disease is 3.70%, and preventive effect is 95.25%, handles apparently higher than NB medium and sterile water.
Embodiment 3CZ preparation is to the tobacco growth-promoting functions
4-5 leaf phase NC89, the slow seedling 5d of small flower transplanting does following processing: irritate root with clear water, NB medium, CZ preparation 10ml respectively, continuous irrigation root 3 times, each 5d at interval, every processing 6 strains, 3 repetitions.Measure fresh weight and maximum leaf length and width behind the 15d, the result is as shown in table 3.
Table 3CZ preparation is to the growth-promoting functions of NC89
By table 3 as seen, CZ irritates root has growth-promoting functions to NC89, and maximum leaf length and width, fresh weight all are higher than the clear water contrast, and difference is extremely remarkable.
The preparation of embodiment 4CZ preparation
CZ, NB, water add polysorbas20 respectively, and the polysorbas20 final concentration is respectively 5%, 3%, 1%, 0%.5-6 leaf three lives phase NN cigarette, 24h sprays above-mentioned processing before the inoculation, and every strain 10ml adds up withered spot inhibiting rate, and the result is shown in table 4, table 5.
Table 4 polysorbas20 suppresses the influence (average withered spot number) of TMV action effect to the CZ zymotic fluid
Table 5 polysorbas20 suppresses the influence (withered spot inhibiting rate %) of TMV action effect to the CZ zymotic fluid
Find out by table 4, add polysorbas20, itself TMV is had inhibitory action, when adding tween final concentration 5% in the water, withered spot number is 52.3, is 170 and do not add the polysorbas20 withered spot number, (table 4).As seen tween has originally influenced the intrusion of virion.
Add polysorbas20 in the CZ zymotic fluid, do not influence the effect of CZ, can consider that the employing polysorbas20 is a spreading agent in the CZ thalline fermentation liquor preparation technology TMV.Polysorbas20 is in conjunction with the lauric polyoxyethylene Span that anhydrates, and is the surface tension material of nonionic, is common cosolvent.It is the big molecule of a class, and existing hydrophilic department on the molecule has the department of oleophylic again, can promote the big molecule that plant absorbing can not melt in water, also can help moisture to see through some and contain the high biomembrane of fat.Add polysorbas20 in the pesticidal preparations, play moistening peptizaiton, other materials are evenly disperseed in solution, increase the stability of emulsion.In solution, can disturb the effect of bacteriostatic agent, but but make non-cultivation conditions bacterium return to cultivation conditions, increase the quantity of bacterium, play the effect of stablizing bacterium, and the albumen that bacterium produced is played a protective role.
The polysorbas20 final concentration is 5% o'clock, and blade has slight poisoning, and therefore, when cooking the microbial inoculum spreading agent with tween, the polysorbas20 final concentration is controlled at below 5%.So final to determine to add the polysorbas20 final concentration be 3% to make CZ thalline preparation.
Among the above embodiment, in CZ preparation, the CZ zymotic fluid, the viable count of pseudomonas fluorescens CZ is 20,000,000,000 spore/milliliters, the acquisition of CZ preparation, CZ zymotic fluid, all obtain, can obtain, therefore do not repeat them here as adopting the method in " Chinese document: " optimal conditions of fermentation of antagonism TMV bacterium CZ and the Primary Study of active substance thereof ", Wu Huihui; Wang Fenglong; Chen Dexin, Chinese tobacco science, 2009; 30 (1): 50-53 page or leaf " by prior art.
Claims (9)
1. Antiphytoviral preparation, it is characterized in that, it is composed of the following components: pseudomonas fluorescens CZ culture fluid, polysorbas20, wherein, in the pseudomonas fluorescens CZ culture fluid, the viable count of pseudomonas fluorescens CZ is 20,000,000,000 spore/milliliters, and the quality of polysorbas20 accounts for 0～5% of preparation gross mass; Described pseudomonas fluorescens CZ called after Pseudomonas fluorescens has been preserved in Chinese common micro-organisms culture presevation administrative center on 08 23rd, 2010, its deposit number is CGMCCNO.4118.
2. a kind of Antiphytoviral preparation according to claim 1, it is characterized in that: the quality of described polysorbas20 accounts for 1～5% of preparation gross mass.
3. a kind of Antiphytoviral preparation according to claim 1, it is characterized in that: the quality of described polysorbas20 accounts for 3% of preparation gross mass.
4. claim 1 or the 2 or 3 described a kind of Antiphytoviral preparations application in the treatment viroses of plant.
5. application according to claim 4 is characterized in that: the application of described preparation in suppressing tobacco virus.
6. application according to claim 4 is characterized in that: the application of described preparation in suppressing tobacco TMV virus disease.
7. claim 1 or 2 or 3 application of described a kind of Antiphytoviral preparation in killing plant virus.
8. claim 1 or the 2 or 3 described a kind of Antiphytoviral preparations application in promoting plant growth.
9. application according to claim 7 is characterized in that: described crop is a tobacco.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102633544A (en) * | 2012-05-09 | 2012-08-15 | 云南省烟草公司保山市公司 | Method for taking farmyard manure as raw material to produce tobacco biofertilizer |
| CN102633565A (en) * | 2012-05-09 | 2012-08-15 | 云南省烟草公司保山市公司 | Method for producing tobacco biofertilizer by utilizing rape seed cake |
| CN106811203A (en) * | 2017-02-27 | 2017-06-09 | 成都康巴金生物技术有限公司 | A kind of application of the preparation method and Pseudomonas fluorescens H4 of biological soil renovation agent |
| CN107236689A (en) * | 2017-06-22 | 2017-10-10 | 中国科学院微生物研究所 | One fluorescent pseudomonads pf27 and its application in plant growth-promoting |
| CN111184029A (en) * | 2019-12-10 | 2020-05-22 | 中国农业科学院烟草研究所 | Application of pseudomonas fluorescens in prevention and removal of dicotyledonous weeds and powdery mildew, and microbial inoculum, preparation method and use method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101372680A (en) * | 2008-10-23 | 2009-02-25 | 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 | Fluorescent pseudomonas for preventing bacterial leaf spot of tobacco |
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2010
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101372680A (en) * | 2008-10-23 | 2009-02-25 | 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 | Fluorescent pseudomonas for preventing bacterial leaf spot of tobacco |
Non-Patent Citations (3)
| Title |
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| 吴惠惠: "一种烟草普通花叶病毒拮抗细菌活性物质的分离纯化及其作用机制研究", 《中国优秀硕士学位论文全文数据库农业科技辑》, no. 10, 15 October 2008 (2008-10-15), pages 046 - 62 * |
| 吴惠惠等: "一种细菌蛋白CZ控制烟草花叶病毒（TMV）的作用机理初探", 《植物保护》, vol. 34, no. 3, 31 December 2008 (2008-12-31), pages 74 - 77 * |
| 吴惠惠等: "拮抗TMV细菌CZ的最佳发酵条件及其活性物质的初步研究", 《中国烟草科学》, vol. 30, no. 1, 31 December 2009 (2009-12-31), pages 50 - 53 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102633544A (en) * | 2012-05-09 | 2012-08-15 | 云南省烟草公司保山市公司 | Method for taking farmyard manure as raw material to produce tobacco biofertilizer |
| CN102633565A (en) * | 2012-05-09 | 2012-08-15 | 云南省烟草公司保山市公司 | Method for producing tobacco biofertilizer by utilizing rape seed cake |
| CN102633544B (en) * | 2012-05-09 | 2014-06-04 | 云南省烟草公司保山市公司 | Method for taking farmyard manure as raw material to produce tobacco biofertilizer |
| CN102633565B (en) * | 2012-05-09 | 2014-06-04 | 云南省烟草公司保山市公司 | Method for producing tobacco biofertilizer by utilizing rape seed cake |
| CN106811203A (en) * | 2017-02-27 | 2017-06-09 | 成都康巴金生物技术有限公司 | A kind of application of the preparation method and Pseudomonas fluorescens H4 of biological soil renovation agent |
| CN106811203B (en) * | 2017-02-27 | 2019-12-24 | 成都康巴金生物技术有限公司 | Preparation method of biological soil remediation agent and application of pseudomonas fluorescens H4 |
| CN107236689A (en) * | 2017-06-22 | 2017-10-10 | 中国科学院微生物研究所 | One fluorescent pseudomonads pf27 and its application in plant growth-promoting |
| CN111184029A (en) * | 2019-12-10 | 2020-05-22 | 中国农业科学院烟草研究所 | Application of pseudomonas fluorescens in prevention and removal of dicotyledonous weeds and powdery mildew, and microbial inoculum, preparation method and use method thereof |
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Application publication date: 20110706 |