CN102101728A

CN102101728A - Method for treating papermaking sewage with microorganism

Info

Publication number: CN102101728A
Application number: CN2009101146495A
Authority: CN
Inventors: 姜明国; 陈远谟; 陆冠颖; 陆富海; 黄进丽
Original assignee: Guangxi University for Nationalities
Current assignee: Guangxi University for Nationalities
Priority date: 2009-12-21
Filing date: 2009-12-21
Publication date: 2011-06-22
Anticipated expiration: 2029-12-21
Also published as: CN102101728B

Abstract

The invention relates to a method for treating papermaking sewage by adding high-flocculation activity Agrobacterium tumefaciens GXUNC1 CGMCC No.3455 into activated active sludge, belonging to the field of intersection of environmental protection and biotechnology. The method is developed on the basis of the traditional active sludge process treatment, i.e. 3-5 percent of exponential growth phase Agrobacterium tumefaciens GXUNC1 bacterium suspension with an OD 620nm value reaching between 10 and 15 is added into the activated active sludge according to the volume ratio; the activated active sludge is aerated at a temperature of 25-28 DEG C so that dissolved oxygen is larger than 2mg/L for more than 18 hours continuously; and CODcr, BOD5 and SS emission values after papermaking sewage is treated are respectively lower than 90mg/L, 30mg/L and 30mg/L, and the chroma is completely removed and reaches the national emission standard. The invention can improve the active sludge treatment capability by adding the high-flocculation activity Agrobacterium tumefaciens without improving equipment investment, strengthen the flocculation activity and shorten the treatment time, thereby reaching the national papermaking sewage emission standard.

Description

A kind of method of microbiological treatment papermaking wastewater

Technical field:

The present invention relates to the microbiological treatment of papermaking wastewater, belong to environment protection and biotechnology crossing domain.

Background technology:

Paper industry is the mainstay of Chinese national economy, also be the more serious industry of contaminate environment simultaneously, it mainly pollutes the middle-stage wastewater that comes from paper-making process, the general name of the middle-stage wastewater waste water that to be the boiling slurry discharged in washing, screening, bleaching and making beating, wherein with black digestion liquid for.Mainly contain chlorlignin, Mierocrystalline cellulose, hemicellulose and all kinds of chemical assistants etc. in the middle-stage wastewater, composition complexity, colourity height, biodegradability are poor, are the source of pollution that cause water body severe contamination and ecotope havoc.

The method that middle-stage wastewater is handled has obtained fast development in recent ten years, mainly is divided into physics method, chemical method, physico-chemical process and biochemical process etc., and often several method makes alternately and is used for improving treatment effect.At present, most paper mills are after employing is carried out pre-treatment with physics or physico-chemical process to the treatment process of middle-stage wastewater, carry out aerobe and handle and anaerobic biological treatment, and necessary need carry out three grades of advanced treatment.Wherein active sludge or biomembrance process have become medium and small sized enterprises' improvement middle-stage wastewater major technology." People's Republic of China's national economy and social development 11th Five-Year Plan outline " and " State Council is about printing and distributing the notice of the comprehensive programme of work of energy-saving and emission-reduction " (No. (2007) 15, promulgated by the State Council) have proposed total emissions of major pollutants and will reduce by 10% target, in order to reach this target, intend CODcr, BOD5 and SS emission limit among the existing paper industry pollution discharge standard GB3544-2001 to be brought up to 90mg/L, 30mg/L, 30mg/L from 150mg/L, 30mg/L, 50mg/L in 2010, and increased chromaticity index.This just makes the treating water of a lot of use current technology (for example traditional materialization+biochemistry) not reach emission standard, and is that the papermaking wastewater advanced treatment of purpose is imperative with further removal pollutent, CODcr, BOD5, SS and colourity.

Summary of the invention:

The objective of the invention is on existing traditional Wastewater Treated by Activated Sludge Process basis, need not to increase facility investment and make papermaking wastewater reach CODcr, BOD5 after treatment and the SS discharge value is lower than 90mg/L, 30mg/L and 30mg/L, colourity is removed fully.

The present invention is a kind of method that improves active sludge treatment papermaking wastewater effect, be on the basis of original activated sludge process, to improve by adding high flocculation activity bacterial classification, promptly add Agrobacterium tumefaciems (Agrobacterium tumefaciens GXUNC1) in the activatory active sludge, this method has improved the active sludge treatment ability, strengthened flocculation activity, shortened the treatment time, thereby made the papermaking wastewater of handling through this method reach discharging standards.

Concrete scheme of the present invention is:

1, strains separation and feature thereof

Agrobacterium tumefaciems GXUNC1 (preserve by China Committee for Culture Collection of Microorganisms common micro-organisms center, numbering: CGMCC No.3455, date: be to separate near the soil paper mill, Nanning on November 18th, 2009), this bacterial strain has good flocculation activity, adds the treatment effect that can significantly improve in the active sludge papermaking wastewater to.Bacterium colony is circular smooth, translucent on the YMA medium of standard, convexity, and the oyster white mucoid colony of protuberance, growth is quick, and lawn is smooth and be the mucus shape, and gramstaining is negative, no gemma, amphitrichous, aerobic median size is shaft-like.Can utilize glucose, semi-lactosi also produces acid, can utilize Citrate trianion, can utilize ammonium salt, nitrite and nitrate to make nitrogenous source, and growth does not rely on somatomedin, can grow on simple mineral salt substratum.Produce mycoderm on the ferric ammonium citrate substratum, optimum growth temperature 25-30 ℃, the suitableeest growth pH value is 5-8.Adopt the universal primer amplification strain gene group DNA of 16SrRNA gene, then the forward and reverse order-checking of universal primer M13, sequencing result is carried out homology relatively with listed 16SrRNA sequence among BLAST software and the Genbank. sequence is correlated result show, the 16SrRNA homology level of isolate GXUNC1 and Agrobacterium tumefaciens reaches more than 99%, morphology and physio-biochemical characteristics in conjunction with bacterial strain, can determine substantially that strain separated GXUNC1 is the bacterium of agrobacterium, called after Agrobacterium tumefaciens GXUNC1.

2, bacterial strain activation

Bacterial classification GXUNC1 is activated 14-20 hour on the YMA medium of standard, the eugonic bacterium colony of picking is transferred in the 200ml liquid nutrient medium and activates, and cultivates 15-20 hour at 200-250rpm under 28 ℃ of temperature condition, makes the logarithmic phase thalline.The liquid culture based component is glucose 5-10g/L, yeast powder 5-15g/L, MgSO47H ₂O 0.2-0.5g/L, papermaking wastewater 10-50ml/L adjusts to 7.0,121 ℃ of sterilizations of pH 20 minutes.

3, enlarged culturing

The vegetative period thalline of taking the logarithm is transferred in the enlarged culturing base according to the ratio of 2-5%, cultivates more than 12 hours at 200-250rpm under 28 ℃ of temperature condition, makes the application bacterial classification.The enlarged culturing based component is glucose 3-5g/L, yeast powder 5-10g/L, MgSO ₄7H ₂O 0.2-0.5g/L, papermaking wastewater 100-200ml/L adjusts to 7.0,121 ℃ of sterilizations of pH 20 minutes.

4, collect thalline

The thalline of enlarged culturing is collected thalline more than 20 minutes through the above rotating speed of 4000rpm is centrifugal, prepares the thalline suspension with physiological saline, adjusts OD _620nmValue reaches between the 10-16, and is standby.

5, papermaking wastewater is handled

Add 3-5% Agrobacterium tumefaciems GXUNC1 thalline in active sludge according to volume ratio, under 25 ℃ of conditions continuous aeration 18-24 hour, dissolved oxygen should be greater than 2mg/L.Water sampling is measured CODcr, BOD5 and SS value during the aeration, and when measured value is lower than 90mg/L, 30mg/L, 30mg/L simultaneously, colourity is removed fully, handles termination.

Embodiment

Embodiment one: paper waste is handled

1, spawn culture

The GXUNC1 bacterial classification is activated 16 hours on the YMA medium of standard, the eugonic bacterium colony of picking is transferred to further activation in the liquid nutrient medium, cultivates 18 hours at 250rpm under 28 ℃ of temperature condition, makes the logarithmic phase thalline.Get the activation thalline and be transferred in the enlarged culturing base, cultivate more than 12 hours at 250rpm under 30 ℃ of temperature condition, make the application bacterial classification according to 3% ratio.Thalline is collected thalline more than 20 minutes through the above rotating speed of 4000rpm is centrifugal, prepares the thalline suspension with physiological saline or distilled water, adjusts the OD620nm value and reaches 12.0, and is standby.

2, papermaking wastewater is handled

100L COD of sewage cr, the BOD5 and the SS that get the paper mill, Nanning are respectively 1540mg/L, 880mg/L, 1300mg/L, add 3L Agrobacterium tumefaciems GXUNC1 thalline suspension and active sludge in sewage according to volume ratio, continuous aeration is 20 hours under 25 ℃ of conditions, dissolved oxygen 3mg/L.Aeration stops measuring CODcr, BOD5 and SS value and colourity and sees Table 1.

Table 1 embodiment one effluent index

Detect index	Agrobacterium tumefaciems+active sludge	Active sludge
			CODcr(mg/L)	78	135
BOD5(mg/L)	19	64
			SS(mg/L)	15	35
Colourity	0	1.3

Embodiment two: papermaking wastewater is handled

1, spawn culture

The GXUNC1 bacterial classification is activated 18 hours on the YMA medium of standard, the eugonic bacterium colony of picking is transferred in the liquid nutrient medium and activates, and cultivates 16 hours at 250rpm under 30 ℃ of temperature condition, makes activatory logarithmic phase thalline.Get the activation thalline and be transferred in the enlarged culturing base, cultivated 14 hours at 250rpm under 30 ℃ of temperature condition, make the application bacterial classification according to 3% ratio.Thalline is collected thalline more than 20 minutes through the above rotating speed of 4000rpm is centrifugal, prepares the thalline suspension with physiological saline, adjusts OD _620nmValue reaches 12.0, and is standby.

2, papermaking wastewater is handled

100L COD of sewage cr, the BOD5 and the SS that get the paper mill, Nanning are respectively 1632mg/L, 920mg/L, 1450mg/L, add 4L Agrobacterium tumefaciems GXUNC1 thalline suspension and active sludge in sewage according to volume ratio, continuous aeration is 20 hours under 25 ℃ of conditions, dissolved oxygen 3mg/L.Aeration stops measuring CODcr, BOD5 and SS value and colourity and sees Table 2.

Table 2 embodiment two effluent index

Detect index	Agrobacterium tumefaciems+active sludge	Active sludge
			CODcr(mg/L)	80	178
BOD5(mg/L)	20	96
			SS(mg/L)	17	55
Colourity	0	1.5

Claims

1. the method for a microbiological treatment papermaking wastewater, it is characterized in that: the Agrobacterium tumefaciems (Agrobacterium tumefaciens GXUNC1 CGMCC No.3455) by adding high flocculation activity are in the activatory active sludge, thereby improve the effect of active sludge treatment papermaking wastewater, shorten the treatment time.

2. according to the described Agrobacterium tumefaciems GXUNC1 of claim 1, it is characterized in that: bacterium colony is for circular smooth on the YMA medium of standard, translucent, convexity, the oyster white mucoid colony of protuberance, growth fast, lawn is smooth and be the mucus shape, and gramstaining is negative, no gemma, amphitrichous, aerobic median size is shaft-like.Can utilize glucose, semi-lactosi also produces acid, can utilize Citrate trianion, can utilize ammonium salt, nitrite and nitrate to make nitrogenous source, and growth does not rely on somatomedin, can grow on simple mineral salt substratum.

3. according to the method for the described a kind of microbiological treatment papermaking wastewater of claim 1, it is characterized in that: the associated products that adds Agrobacterium tumefaciems GXUNC1 or bacterium generation is thus handled sewage in the activatory active sludge.

4. according to the described a kind of method of making microbiological treatment paper sewage of claim 1, it is characterized in that: handled sewage comprises all than papermaking wastewater more easy to handle industrial sewage and sanitary sewage.