CN102093957A - High-fat Chlorella culture solution for promoting rapid growth and preparation method thereof - Google Patents
High-fat Chlorella culture solution for promoting rapid growth and preparation method thereof Download PDFInfo
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- CN102093957A CN102093957A CN2010105933011A CN201010593301A CN102093957A CN 102093957 A CN102093957 A CN 102093957A CN 2010105933011 A CN2010105933011 A CN 2010105933011A CN 201010593301 A CN201010593301 A CN 201010593301A CN 102093957 A CN102093957 A CN 102093957A
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Abstract
The invention relates to a high-fat Chlorella culture solution for promoting rapid growth and a preparation method thereof, belonging to the field of a biotechnology. The culture solution comprises the following components in percent by weight: 1-8% of bamboo vinegar, 4-15% of macronutrient, 0.2-0.5% of micronutrient, 0-1.5% of soil extract and 78-94% of water, wherein the macronutrient is one of sodium nitrate, dipotassium hydrogen phosphate, potassium nitrate, magnesium sulfate, calcium chloride, potassium dihydrogen phosphate and sodium chloride or a mixture thereof, and the micronutrient is one of ferric chloride, boric acid, copper sulfate, manganese sulfate, zinc sulfate and ammonium molybdate or mixture thereof. Since the bamboo vinegar serving as a synergic ingredient is added into the culture solution, the content of the fat generated during the process of the carbon dioxide immobilization using algae is increased. The high-fat Chlorella culture solution is simple in preparation method, low in production cost, and convenient in operation, and can be used for greatly increasing the content of the fat in Chlorella; and under the same conditions, the purity of the cultured algae is very high, and the culture cost for large-scale exploitation and utilization of the Chlorella is greatly reduced.
Description
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Technical field
The invention belongs to biological technical field, be specifically related to high fat chlorella nutrient solution of a kind of quick promotion growth and preparation method thereof.
Background technology
Chlorella (Chlorella) is the general natural disposition unicell green alga of Chlorophyta Chlorella, it is the unicellular fresh water algae of a kind of sphere, 3～8 microns of diameters, be the earliest one of life on the earth, before appearing at more than 20 hundred million years, gene does not change all the time, is a kind of photosynthetic efficiently plant, with the photoautotrophy growth and breeding, distributed pole is wide.It is a kind of minimum grade, the most ancient plant, and its breeding is fast, and the oil length height is the important source of biofuel.Chlorella contains rich in protein, lipid, polysaccharide, food fiber, VITAMIN, trace element and active metabolite, have the peptide ulceration of control, antitumor, enhancing immunity, radioprotective, resisting pathogenic microbes, prevent and treat health care and pharmacological actions such as anaemia, reducing blood-fat and atherosclerosis, can supply people's safe edible.Chlorella be found at present near the nutritive food of human needs's natural balance.Owing to contain outside the nutritive substances such as rich in protein, polysaccharides, β-Hu Luobusu, gamma-linolenic acid, also be rich in the chlorophyll of getting rid of objectionable impurities in intestines, kidney, lung and the blood, particularly contain somatomedin (CGF), can obviously delay senility, activating human body cells is repaired impaired gene.It is said, in Japan, the taking the first place in all healthcare products are sold in continuous 20 years of chlorella series product.
Chlorella can produce the lipid of 10%～30% dry cell weight under different culture condition, can be used to be converted into the i.e. production biofuel of Fatty acid methyl ester.In addition, chlorella possesses degradable organic pollutant, efficiently adsorbs functions such as heavy metal and absorbed nitrogen phosphorus, has the important use DEVELOPMENT PROSPECT at bioenergy and field of environment protection, is a kind of extremely important little algae resource.Usually use SE substratum (SE Medium) and Pr substratum (Pr Medium), also can use BG-11 substratum (BG-11 Medium) to cultivate.The chlorella autotrophy is cultivated main finger and is utilized luminous energy that inorganic carbon is converted into the process of carbohydrate, and illumination can be adopted sunlight or source of artificial light, and inorganic carbon source is rich will to comprise carbonate or C0
225 ℃～35 ℃ of the suitableeest culture temperature of chlorella, optimal pH 6.5～7.5, in addition optimal light intensity 2000～50001ux, also need provide nitrogenous source, phosphorus source, magnesium and iron and trace element etc. in substratum.At present, the technology that the chlorella autotrophy is cultivated is ripe, but the problem that exists is to stop that after chlorella cells is increased to finite concentration light enters culture, has therefore limited the photosynthesis of chlorella, causes the chlorella biomass cultivated low.Nineteen fifty-three, Lewin has found that at first some algae can utilize organism to carry out heterotrophic growth as the unique carbon source and the energy, thereby has caused the once important revolution of unit cell algae culture.Particularly by the research and discovery of heterotrophic fermentation culture technique, increased substantially the speed of growth of chlorella in recent years, the last industrialization production that chlorella heterotrophy is cultivated becomes possibility.Endo and Shirota elm have been surveyed chlorella and have utilized the ability that comprises more than 60 kind of organic carbon sources such as sugar, organic acid and alcohols, and the result shows that glucose, acetate and ethanol etc. can be used as the growth that sole carbon source is supported chlorella.It is very fast that heterotrophism is cultivated the chlorella cells division, makes more that multipotency is used for respiratory metabolism, limited the synthetic of cellular material, causes its albumen and pigment content to descend.But compare with autotrophy, the lipid content that heterotrophism is cultivated chlorella obviously increases, and helps extracting lipid conversion biodiesel wherein.Barbier finds that some growth hormone can improve the chlorella protein content, its mechanism is that the growth hormone receptor in the frustule nuclear combines with the exogenous auxin of interpolation, formed the startup of " growth hormone---acceptor " mixture identification gene, the ability of chlorella expressing protein content is improved.Li Shi father-in-law finds that sodium acetate and naphthylacetic acid make chlorella internal protein content bring up to 46.9% and 52.5% by 43.4% of contrast, and in addition, the increase of carbonate concentration also helps to improve the chlorella chlorophyll content.Yan Meijiao report white light has promoter action to the growth of chlorella, and ruddiness and blue light can improve the chlorella chlorophyll content.Sansawa etc. carry out the heterotrophism synchronized culture to chlorella, and making albumen in the frustule and chlorophyll content is 2～3 times of non-synchronized culture, illustrate that the control by the heterotrophism cultivation can obtain high-quality chlorella equally.
Exhaustion day by day along with the main Nonrenewable resources oil of the earth has constituted serious threat to human existence and Sustainable development.Therefore utilize fine algae fixation of C 0
2The lipid conversion biodiesel that produces just reaches the purpose of efficient reduction of discharging production capacity.Miu Xiaoling finds that the lipid content of cultivating chlorella by heterotrophism reaches 55% of dry cell weight, it is 4 times that autotrophy is cultivated algae lipid content 14%, the bio oil calorific value that the heterotrophism frustule transforms is respectively timber biological oil and autotrophy algae bio oil 2 times and 1.4 times up to 41MJ/kg.Because it is the glucose that competition takes place with human grain resource that heterotrophism is cultivated the required carbon source of chlorella, therefore at first adopt heterotrophism to cultivate and obtain superelevation cell concn chlorella, change white foster training method fixation of C 0 then into
2, can alleviate CO on the one hand
2The pressure that reduces discharging can obtain a large amount of chlorella cells on the other hand and be used to extract wherein lipid conversion biodiesel.In addition, little algae directly degradable organic pollutant, efficiently adsorb heavy metal and absorbed nitrogen phosphorus, for the recycling treatment of waste water provides a kind of new thinking.
Summary of the invention
At the above-mentioned problems in the prior art, the objective of the invention is to design the technical scheme of high fat chlorella nutrient solution that a kind of quick promotion growth is provided and preparation method thereof, its production cost is low, and is easy to operate, promote that the chlorella lipid content is effective, cultivate and obtain algae purity height.
The high fat chlorella nutrient solution of described a kind of quick promotion growth, it is characterized in that containing the following material of weight percentage: bamboo vinegar liquid 1-8%, main nutrient 4-15%, micro-nutrient 0.2-2.5%, soil extract 0-1.5%, water 78-94%, described main nutrient is one or more mixtures in SODIUMNITRATE, dipotassium hydrogen phosphate, saltpetre, sal epsom, calcium chloride, potassium primary phosphate, the sodium-chlor, and described micro-nutrient is one or more mixtures in iron(ic) chloride, boric acid, copper sulfate, manganous sulfate, zinc sulfate, the ammonium molybdate.
The high fat chlorella nutrient solution of described a kind of quick promotion growth is characterized in that the content of described bamboo vinegar liquid is 2-7%, is preferably 3-5%.
The high fat chlorella nutrient solution of described a kind of quick promotion growth is characterized in that the content of described main nutrient is 6-12%, is preferably 7-10%.
The high fat chlorella nutrient solution of described a kind of quick promotion growth is characterized in that the content of described micro-nutrient is 0.5-2.0%, is preferably 0.8-1.5%.
The high fat chlorella nutrient solution of described a kind of quick promotion growth, the content that it is characterized in that described soil extract is 0.2-1.0%, preferred 0.4-0.8%.
The high fat chlorella nutrient solution of described a kind of quick promotion growth, the content that it is characterized in that described water is 82-90%, preferred 85-88%.
The preparation method of the high fat chlorella nutrient solution of described a kind of quick promotion growth is characterized in that may further comprise the steps:
1) gets and do not execute overplumped soil 500g and place container, add distilled water 1000ml, bottleneck seals with porous plug, the boiling water heating is 2-4 hour in water-bath, cools off three one-tenth filtered through gauze under aseptic condition then 4-6 hour, get supernatant liquor, the distilled water of bacterium of will going out again adds in the supernatant liquor to cumulative volume 1000ml, is soil extract, and 3-6 ℃ of preservation is standby;
2) in reactor, drop into bamboo vinegar liquid 1-8%, main nutrient 4-15%, micro-nutrient 0.2-2.5%, soil extract 0-1.5%, water 78-94%, stir the finished product.
Described bamboo vinegar liquid is the by product that bamboo surplus material of processing obtains through high temperature pyrolysis, can directly buy from the market.
The high fat chlorella nutrient solution of above-mentioned a kind of quick promotion growth is made synergistic component by adding bamboo vinegar liquid, has improved algae fixation of C 0 greatly
2The lipid content that produces, its preparation method is simple, and production cost is low, and is easy to operate, promotes that the chlorella lipid content is effective, and under square one, it is very high that cultivation obtains algae purity, reduced the cultivation cost that chlorella is large-scale developed and utilized significantly.
The percentage composition that relates in the present specification unless otherwise indicated, is weight percentage.
Embodiment
Now, further specify beneficial effect of the present invention in conjunction with embodiments of the invention and culture experiment.
Embodiment 1
In the 1000L stirring tank, drop into the 30kg bamboo vinegar liquid, add 15kg SODIUMNITRATE, 20kg saltpetre, 15kg potassium primary phosphate and 15kg sodium-chlor again, add 1kg iron(ic) chloride, 1kg boric acid, 1kg copper sulfate, 1kg manganous sulfate, 0.5kg zinc sulfate and 0.5kg ammonium molybdate then, stir, add 900kg water again, be stirred to all substances dissolvings, the finished product.
Embodiment 2
In the 1000L stirring tank, drop into the 50kg bamboo vinegar liquid, add 25kg saltpetre, 25kg sal epsom, 25kg calcium chloride and 25kg sodium-chlor again, add 10kg iron(ic) chloride, 5kg boric acid and 5kg copper sulfate then, stir, add the 10kg soil extract, add 820kg water again, be stirred to all substances dissolvings, the finished product.
Embodiment 3
In the 1000L stirring tank, drop into the 38kg bamboo vinegar liquid, add 17kg SODIUMNITRATE, 17kg dipotassium hydrogen phosphate, 17kg calcium chloride, 17kg potassium primary phosphate and 17kg sodium-chlor again, add 2kg copper sulfate, 2kg manganous sulfate, 2kg zinc sulfate and 2kg ammonium molybdate then, stir, add the 2kg soil extract, add 867kg water again, be stirred to all substances dissolvings, the finished product.
Embodiment 4
In the 1000L stirring tank, drop into the 35kg bamboo vinegar liquid, add 16kg dipotassium hydrogen phosphate, 16kg saltpetre, 16kg sal epsom, 16kg calcium chloride and 16kg potassium primary phosphate again, add 5kg iron(ic) chloride, 5kg copper sulfate, 2.5kg manganous sulfate and 2.5kg ammonium molybdate then, stir, add the 4kg soil extract, add 866kg water again, be stirred to all substances dissolvings, the finished product.
Embodiment 5
In the 1000L stirring tank, drop into the 40kg bamboo vinegar liquid, add 5kg SODIUMNITRATE, 10kg dipotassium hydrogen phosphate, 30kg saltpetre and 30kg sal epsom again, add 2.5kg boric acid, 2.5kg copper sulfate, 2.5kg manganous sulfate and 2.5kg zinc sulfate then, stir, add the 1kg soil extract, add 874kg water again, be stirred to all substances dissolvings, the finished product.
Embodiment 6
In the 1000L stirring tank, drop into the 48kg bamboo vinegar liquid, add 10kg SODIUMNITRATE, 10kg dipotassium hydrogen phosphate, 10kg saltpetre, 10kg sal epsom, 10kg calcium chloride, 10kg potassium primary phosphate and 10kg sodium-chlor again, add 3kg iron(ic) chloride, 3kg boric acid, 3kg copper sulfate, 3kg manganous sulfate, 3kg zinc sulfate and 3kg ammonium molybdate then, stir, add the 8kg soil extract, add 856kg water again, be stirred to all substances dissolvings, the finished product.
Below by the promotion growth result of test explanation nutrient solution, test-results sees Table 1.
Table 1 embodiment 1-6 is to the influence of chlorella biomass (OD680nm)
CK be meant except that with the embodiment substratum in do not have the bamboo vinegar liquid, all the other compositions are all identical, be that the substratum of CK1 does not have bamboo vinegar liquid and embodiment 1 has bamboo vinegar liquid, all the other compositions are all identical with content, CK2 and embodiment 2, CK3 and embodiment 3, CK4 and embodiment 4, CK5 and embodiment 5, CK6 and embodiment 6, same understanding.From last table 1 as seen, the substratum that contains bamboo vinegar liquid has the effect of obvious promotion chlorella growth.
Claims (7)
1. high fat chlorella nutrient solution that promotes fast growth, it is characterized in that containing the following material of weight percentage: bamboo vinegar liquid 1-8%, main nutrient 4-15%, micro-nutrient 0.2-2.5%, soil extract 0-1.5%, water 78-94%, described main nutrient is one or more mixtures in SODIUMNITRATE, dipotassium hydrogen phosphate, saltpetre, sal epsom, calcium chloride, potassium primary phosphate, the sodium-chlor, and described micro-nutrient is one or more mixtures in iron(ic) chloride, boric acid, copper sulfate, manganous sulfate, zinc sulfate, the ammonium molybdate.
2. the high fat chlorella nutrient solution of a kind of quick promotion growth as claimed in claim 1 is characterized in that the content of described bamboo vinegar liquid is 2-7%, is preferably 3-5%.
3. the high fat chlorella nutrient solution of a kind of quick promotion growth as claimed in claim 1 is characterized in that the content of described main nutrient is 6-12%, is preferably 7-10%.
4. the high fat chlorella nutrient solution of a kind of quick promotion growth as claimed in claim 1 is characterized in that the content of described micro-nutrient is 0.5-2.0%, is preferably 0.8-1.5%.
5. the high fat chlorella nutrient solution of a kind of quick promotion growth as claimed in claim 1, the content that it is characterized in that described soil extract is 0.2-1.0%, preferred 0.4-0.8%.
6. the high fat chlorella nutrient solution of a kind of quick promotion growth as claimed in claim 1, the content that it is characterized in that described water is 82-90%, preferred 85-88%.
7. as the preparation method of a kind of high fat chlorella nutrient solution of quick promotion growth as described in the right 1, it is characterized in that may further comprise the steps:
1) gets and do not execute overplumped soil 500g and place container, add distilled water 1000ml, bottleneck seals with porous plug, the boiling water heating is 2-4 hour in water-bath, cools off three one-tenth filtered through gauze under aseptic condition then 4-6 hour, get supernatant liquor, the distilled water of bacterium of will going out again adds in the supernatant liquor to cumulative volume 1000ml, is soil extract, and 3-6 ℃ of preservation is standby;
2) in reactor, drop into bamboo vinegar liquid 1-8%, main nutrient 4-15%, micro-nutrient 0.2-2.5%, soil extract 0-1.5%, water 78-94%, stir the finished product.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104087527A (en) * | 2014-06-20 | 2014-10-08 | 沈阳壅源生物有机肥有限公司 | Bacillus thuringiensis, preparation method for solid medium of Bacillus thuringiensis, and application of Bacillus thuringiensis |
| CN104496654A (en) * | 2014-12-03 | 2015-04-08 | 东莞市绿安奇生物工程有限公司 | Method for preparing and using low-heavy-metal-content chlorella cultivation raw material mother solution |
| CN108795839A (en) * | 2018-07-02 | 2018-11-13 | 中国科学院成都生物研究所 | A kind of method of stem of noble dendrobium single-cell suspension culture |
| CN114164116A (en) * | 2021-12-15 | 2022-03-11 | 海南大学 | Chlorella culture method |
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| JPS60180578A (en) * | 1984-02-27 | 1985-09-14 | Shinichi Ito | Chlorella cultivation liquid and cultivation method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104087527A (en) * | 2014-06-20 | 2014-10-08 | 沈阳壅源生物有机肥有限公司 | Bacillus thuringiensis, preparation method for solid medium of Bacillus thuringiensis, and application of Bacillus thuringiensis |
| CN104496654A (en) * | 2014-12-03 | 2015-04-08 | 东莞市绿安奇生物工程有限公司 | Method for preparing and using low-heavy-metal-content chlorella cultivation raw material mother solution |
| CN108795839A (en) * | 2018-07-02 | 2018-11-13 | 中国科学院成都生物研究所 | A kind of method of stem of noble dendrobium single-cell suspension culture |
| CN108795839B (en) * | 2018-07-02 | 2022-02-11 | 中国科学院成都生物研究所 | Method for suspension culture of dendrobium single cells |
| CN114164116A (en) * | 2021-12-15 | 2022-03-11 | 海南大学 | Chlorella culture method |
| CN114164116B (en) * | 2021-12-15 | 2023-10-24 | 海南大学 | Chlorella culture method |
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