CN102091344A - 一种金纳米棒-壳聚糖薄膜及其制备方法 - Google Patents

一种金纳米棒-壳聚糖薄膜及其制备方法 Download PDF

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CN102091344A
CN102091344A CN2010105474059A CN201010547405A CN102091344A CN 102091344 A CN102091344 A CN 102091344A CN 2010105474059 A CN2010105474059 A CN 2010105474059A CN 201010547405 A CN201010547405 A CN 201010547405A CN 102091344 A CN102091344 A CN 102091344A
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chitosan
gold nanorods
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CN102091344B (zh
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韩志超
许杉杉
李立藏
申孟芝
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Wuxi Zhongke Guangyuan Biomaterials Co Ltd
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Abstract

本发明公开了一种金纳米棒-壳聚糖薄膜及其制备方法，其中，所述金纳米棒-壳聚糖薄膜按重量百分比含有92～98％的金纳米棒以及2～8％的壳聚糖，先将原料制成膜溶液后进行浇注、干燥以及碱化等步骤制得本发明的金纳米棒-壳聚糖薄膜。本发明的金纳米棒-壳聚糖薄膜具有良好的化学通用性以及生理稳定性，且激光辐照率高，在诊断和治疗癌症方面有巨大潜力；另外，所述薄膜具有良好的生物相容性，通过激光激活可使得薄膜具有理想的粘附性能，从而成为伤口敷料、组织修复和局部药物释放的理想选择材料。

Description

一种金纳米棒-壳聚糖薄膜及其制备方法
技术领域
本发明涉及纳米生物膜材料及其制备方法，更具体地，是一种金纳米棒-壳聚糖薄膜及其制备方法。
背景技术
壳聚糖是一种线性链状多聚糖，由葡萄糖胺和N-乙酰葡萄糖胺残基通过β-1，4-糖苷键连接组成。壳聚糖由酸溶性材料甲壳素即平时发现的甲壳类动物外骨骼和真菌的细胞壁脱乙酰得到。壳聚糖的基本属性具有良好的生物相容性和生物降解性，从而使得壳聚糖成为非常有吸引力的生物医学应用材料，如伤口敷料、组织工程和药物释放等。另外，壳聚糖特有的性质使其形成的薄膜具有机械强度高，弹性好等优点。目前，壳聚糖薄膜已经在局部化疗——肿瘤切除后抗肿瘤药物的局部稳定释放，以及各种外伤口的组织修复中进行应用，在应用中，壳聚糖薄膜需要固定在作用目标上，但是在诸如颅内，心肌等处应用时，缝合固定难以实现。另外，在壳聚糖薄膜意外植入金属和合成材料会干扰创伤部位的康复，异物反应也可能引起发炎。因此，提高壳聚糖薄膜的生物相容性以及生理稳定性等是本领域亟待解决的问题。
发明内容
本发明目的在于提供一种生物相容性好的金纳米棒-壳聚糖薄膜及其制备方法。
本发明是通过如下技术手段实现的：针对金纳米棒具有良好的化学通用性和生理稳定性以及高效的激光辐照率，壳聚糖具有良好的生物相容性以及可生物降解性，将金纳米棒和壳聚糖配制成溶液后通过浇注、干燥以及碱化等步骤，提供一种具有生物相容性好且通过激光激活后粘附性能强的金纳米棒-壳聚糖生物薄膜，是伤口敷料、组织修复以及局部药物释放等的一个理想选择，且制备工艺简单、成本低。
本发明的金纳米棒-壳聚糖薄膜，按重量百分比含有92～98％的金纳米棒以及2～8％的壳聚糖。
本发明的制备上述金纳米棒-壳聚糖薄膜的方法，包括如下步骤：
(1)配制膜溶液：先将金纳米粒子分散于乙酸中制成浓度为0.5×10-10～8×10-10M的分散液，再将低分子量的脱乙酰度为70～95％的壳聚糖溶解于所述分散液中，得到壳聚糖的质量体积浓度为0.5～8％的膜溶液；
(2)制膜：每次取20～10000μL的上述膜溶液在1～100cm2的聚苯乙烯圆形模具中浇注，然后在10～50℃下干燥6～48小时，得到粗品，将所述粗品置于浓度为1M以上的氢氧化钠中碱化2～120分钟并用清水洗净后得到金纳米棒-壳聚糖薄膜，保存于4℃的蒸馏水中。
按照本发明制备的金纳米棒-壳聚糖薄膜具有良好的化学通用性和生理稳定性以及高效的激光辐照率，通过激光活化后具有良好的粘附性能，与其良好的生物相容性结合后可用于伤口敷料、组织修复以及局部药物释放等材料；本发明的制备方法工艺简单、操作方便，有效节约了实施成本。
具体实施方式
以下描述本发明的优选实施方式，但并非用以限定本发明。
实施例1：
按照如下方法制备金纳米棒-壳聚糖薄膜：
(1)配制膜溶液：先将金纳米粒子分散于乙酸中制成浓度为2×10-10M的分散液，再将低分子量的脱乙酰度为70～95％的壳聚糖溶解于所述分散液中，得到壳聚糖的质量体积浓度为2％的膜溶液；
(2)制膜：每次取150μL的上述膜溶液在50cm2的聚苯乙烯圆形模具中浇注，然后在50℃下干燥12小时，得到粗品，将所述粗品置于浓度为1.5M的氢氧化钠中碱化10分钟并用清水洗净后得到金纳米棒-壳聚糖薄膜，保存于4℃的蒸馏水中。
实践证明，本发明的金纳米棒-壳聚糖薄膜具有良好的化学通用性和生理稳定性以及高效的激光辐照率，通过激光活化后具有良好的粘附性能，与其良好的生物相容性结合后可用于伤口敷料、组织修复以及局部药物释放等材料，且制备工艺简单、操作简便。

Claims (2)

1.一种金纳米棒-壳聚糖薄膜，其特征在于，按重量百分比含有92～98％的金纳米棒以及2～8％的壳聚糖。
2.一种制备权利要求1所述的金纳米棒-壳聚糖薄膜的方法，其特征在于，包括如下步骤：
(1)配制膜溶液：先将金纳米粒子分散于乙酸中制成浓度为0.5×10-10～8×10-10M的分散液，再将低分子量的脱乙酰度为70～95％的壳聚糖溶解于所述分散液中，得到壳聚糖的质量体积浓度为0.5～8％的膜溶液；
(2)制膜：每次取20～10000μL的上述膜溶液在1～100cm2的聚苯乙烯圆形模具中浇注，然后在10～50℃下干燥6～48小时，得到粗品，将所述粗品置于浓度为1M以上的氢氧化钠中碱化2～120分钟并用清水洗净后得到金纳米棒-壳聚糖薄膜，保存于4℃的蒸馏水中。
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