CN102028745B

CN102028745B - Drug for treating coronary heart disease and preparation process

Info

Publication number: CN102028745B
Application number: CN2009100706856A
Authority: CN
Inventors: 刘顺航; 徐波; 戚可人; 刘岩; 陈红; 丛德刚; 赵国辉
Original assignee: TIANJIN TASLY MODERN CHINESE MEDICINE RESOURCE CO Ltd
Current assignee: TIANJIN TASLY MODERN CHINESE MEDICINE RESOURCE CO Ltd
Priority date: 2009-09-29
Filing date: 2009-09-29
Publication date: 2013-12-11
Anticipated expiration: 2029-09-29
Also published as: CN102028745A

Abstract

The invention relates to a drug for treating coronary heart disease and a preparation process. The preparation method comprises the following steps: extracting salvia and notoginseng with alcohol, extracting dregs of salvia with alcohol and the like. The invention has the following advantages: the process route can realize industrialization and the quality is stable and controllable. Relevant experiments show that the drug has the advantages of high curative effect and product purity, good absorbability, stable quality and novel application compared with the prior art, and meanwhile, the preparation method is simple in process, convenient to operate, low in cost and suitable for industrial production.

Description

A kind of medicine and preparation technology who treats coronary heart disease

Technical field

The present invention relates to a kind of medicine for the treatment of coronary heart disease, belong to the field of Chinese medicines.

Background technology

Along with the rejuvenation of growth in the living standard, world population aging and morbidity colony, Patients with geriatric cardiovascular and cerebrovascular diseases increases year by year, has become the second largest disease of harm humans health.Angina pectoris is a kind of caused by the temporary transient ischemia of cardiac muscle, anoxia, take the clinical syndrome that ictal chest pain or chest discomfort be main manifestations.Angina pectoris refers to because coronary atherosclerosis or spasm cause myocardial ischemia, the caused angina pectoris of anoxia, accounts for 90% of patient with angina pectoris.

The anginal method for the treatment of be take blood vessel dilating, reduction blood viscosity, anti-platelet aggregation, anticoagulation as main at present.Traditional Western medicine of application is nitrate, nitrous acid ester, beta-blocker, calcium antagonist etc., but all has larger toxic and side effects, unsuitable long-term taking, mostly be symptomatic treatment and to course advancement without larger effect.For example take after nitroglycerin occur sometimes beating in feeling of fullness in the head, head, palpitating speed, even faint [referring to 264 pages of new pharmacologies (the 14th edition)], find that there is again in recent years and cause severe hypotension [referring to contemporary Chinese medical journal 1997; 7 (4): 42; Shaanxi medical journal 1996; 25 (5): 315], easily produce toleration [referring to southern nursing magazine 1996; 3 (5): 7～9] etc. problem, hindered its application clinically.

Although the anginal Chinese patent medicine of many treatments is also arranged, wherein ball, loose, cream, pellet, decoction become ancient history already, and the modern seldom applies.There are in the market the preparations such as common FUFANG DANSHEN PIAN and capsule to sell, but conventional tablet, capsule manufacture technique fall behind, active constituent content is low, without quality control index, need oral through gastrointestinal absorption, absorbed into serum after liver generation first pass effect, bioavailability is low, absorbs slow. can not be applicable to the need of the first aid of Patients With Angina Pectoris.

FUFANG DANSHEN DIWAN is fast, the eutherapeutic therapeutic drug of coronary heart disease of a kind of special effect, deeply is subject to clinically patient's welcome.In order to improve the preparation technology of FUFANG DANSHEN DIWAN, the inventor conducts in-depth research from the extraction process aspect, from saving the herb resource aspect, has improved product yield, the comprehensive utilization ratio of the contained effective ingredient of medical material.

Summary of the invention

The invention provides a kind of medicine for the treatment of coronary heart disease.

The medicine for the treatment of coronary heart disease of the present invention, described medicine is prepared from by the crude drug of following percentage by weight, Radix Salviae Miltiorrhizae 19.8%-97%, Radix Notoginseng 2%-80%, Borneolum Syntheticum 0.2%-3%, described preparation is first crude drug to be prepared into to active constituents of medicine through following steps, more further is prepared into medicine.

Step 1, Radix Salviae Miltiorrhizae adds alcohol, extracts, and filters, and extracting solution is concentrated, standing, filters, and precipitation is dry, obtains first's tanshinone ⅡA extract; Filtrate is crossed resin, first uses low concentration alcohol eluting, then uses high concentration alcohol eluting, collects the high concentration alcohol eluen, concentrated, standing, filters, and precipitation is dry, obtains second portion tanshinone ⅡA extract, and two parts merge, and obtain Tanshinone I I A extract;

Step 2, get step 1 Radix Salviae Miltiorrhizae alcohol extraction medicinal residues, extracts, and filters, and filtrate is concentrated, lets cool, acid adding is regulated pH value, and cold preservation is standing, filters, and filtrate is crossed resin, washes successively low concentration alcohol eluting with water, high concentration alcohol eluting, collect the high concentration alcohol eluen, and concentrated, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds alcohol, extracts, and extracting solution is concentrated, dry, obtains Radix Notoginseng extract;

Step 4, the salvianolic acid B medicinal residues after step 2 alcohol extraction add, and aqueous alkali is or/and water extraction, and extracting solution is concentrated, adds alcohol, and standing, supernatant concentration, obtain Radix Salviae Miltiorrhizae extractum;

Step 5, the Tanshinone I I A extract that step 1 obtains, the salvianolic acid B extract that step 2 obtains, the Radix Notoginseng extract that step 3 obtains, the Radix Salviae Miltiorrhizae extractum that step 4 obtains and Borneolum Syntheticum are further made drop pill.

Medicine of the present invention is for oral medicine, its preparation adopts the preparation of galenic pharmacy routine techniques, comprise the step that active constituents of medicine is mixed with the adjuvant of oral drugs, wherein the adjuvant of oral drug preparation is selected from starch, amylum pregelatinisatum, dextrin, Icing Sugar, lactose, mannitol, calcium sulfate two water things, calcium hydrogen phosphate, magnesium oxide, calcium carbonate, magnesium carbonate, water, ethanol, starch slurry, syrup, liquid glucose, maltose, refined honey, cane sugar powder, citric acid, essence, mucialga of arabic gummy, gelatine size, polyvinylpyrrolidone (PVP), rubber cement, microcrystalline Cellulose, sodium carboxymethyl cellulose, hydroxypropyl emthylcellulose, magnesium stearate, stearic acid, zinc stearate, calcium stearate, Pulvis Talci, Macrogol 4000, polyethylene glycol 6000, micropowder silica gel, vegetable oil, sodium stearate, glycerin gelatine, stearic acid, glyceryl monostearate, insect wax, one or several combinations of hydrogenated oil and fat.

Oral formulations of the present invention is selected from: all acceptable dosage forms on tablet, dispersible tablet, hard capsule, soft capsule, granule, pill, micropill, powder, drop pill, slow releasing preparation, controlled release preparation, syrup, oral liquid, soft extract and extractum pharmaceutics.

Its preferred preparation method of medicine that confession of the present invention is oral, step is:

Step 1, Radix Salviae Miltiorrhizae adds 2-20 and doubly measures 60%～100% alcohol extraction 1-10 hour, filters, and medicinal residues are doubly measured 60%～100% alcohol with 2-20, extract 1-10 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.2-5 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; Macroporous resin on filtrate (polar resin S-8, low pole Resin A B-8 or non-polar resin D101), the 50-85% ethanol elution, eluent discards, use again the 85-100% ethanol elution, collect eluent, be concentrated into containing alcohol amount 65-85%, standing, filter, the precipitation drying under reduced pressure, obtain second portion Tanshinone I I A extract, two parts merge, and obtain the tanshinone ⅡA extract;

Step 2, get step 1 Radix Salviae Miltiorrhizae alcohol extraction medicinal residues and add 2-20 and doubly measure 10-100% alcohol extraction 0.5-10 hour, filter, last medicinal residues add 2-20 and doubly measure alcohol extraction 0.5-10 hour, filter, filtrate is concentrated into without alcohol, lets cool to below 10 ℃, adds dilute hydrochloric acid to liquid PH value 1-4, standing, filter, filtrate is crossed macroporous resin (polar resin S-8, low pole Resin A B-8, non-polar resin D101 or polyamide), wash successively low concentration alcohol eluting, high concentration alcohol eluting with water, collect the high concentration alcohol eluen, concentrated, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds methanol, ethanol, normal propyl alcohol, isopropyl alcohol, n-butyl alcohol or the isobutanol of 2-20 times of 50-100%, reflux, extract, 2 times, each extraction time 0.5-10 hour, filter, and extracting solution concentrating under reduced pressure, drying, obtain Radix Notoginseng extract;

Step 4, the salvianolic acid B medicinal residues after the alcohol extraction of step 2, add the aqueous alkali of 2-12 times of pH value 7-10, extract 1-3 time, add again 2-12 times of water, extract 1-3 time, filter, filtrate is concentrated, add ethanol to medicinal liquid and measure 50-80% containing alcohol, standing, separation of supernatant, reclaim ethanol, receive cream to pol 50%-90% or concentrated, dry, pulverizing, obtain Radix Salviae Miltiorrhizae extractum;

Step 5, by tanshinone ⅡA extract obtained above, salvianolic acid B extract, Radix Notoginseng extract, Radix Salviae Miltiorrhizae extractum mixes with adjuvant with Borneolum Syntheticum, adds any or more than one adjuvants, the 1-6 that the adjuvant addition is active component doubly, makes any oral formulations;

Wherein described in step 1 and 2, alcohol is selected from: methanol, ethanol, normal propyl alcohol, isopropyl alcohol, n-butyl alcohol or isobutanol alcohol, described extracting mode is selected from: reflux, extract,, warm macerating extraction, merceration extraction, percolation extraction, supersound extraction, dynamic countercurrent extraction or microwave extraction

The preferred drop pill of medicine of the present invention, its preparation adopts the preparation of galenic pharmacy routine techniques, comprises the step that active constituents of medicine is mixed with the adjuvant of drop pill medicine, and described adjuvant is selected from fat-soluble substrate and water-soluble base, and the auxiliary ratio of medicine is 1: 1-6.Wherein said fat-soluble substrate is selected from: stearic acid, glyceryl monostearate, insect wax, Cera Flava, paraffin, hydrogenated vegetable oil, semi-synthetic fatty acid ester; Water-soluble base is selected from Polyethylene Glycol, polyoxyethylene monostearate, poloxamer, sodium stearate, glycerin gelatine etc.; Xylitol and composites of starch, erythritol; Preferably the auxiliary ratio of medicine is 1: 3.0.

The preferred preparation method of drop pill of the present invention, step is:

Step 1, Radix Salviae Miltiorrhizae adds 2-15 and doubly measures 60%～100% alcohol extraction 1-8 hour, filters, and medicinal residues are doubly measured 60%～100% alcohol with 2-15, extract 1-8 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.2-3 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; Macroporous resin on filtrate (polar resin S-8, low pole Resin A B-8 or non-polar resin D101), the 50-85% ethanol elution, eluent discards, use again the 85-100% ethanol elution, collect eluent, be concentrated into containing alcohol amount 65-85%, standing, filter, the precipitation drying under reduced pressure, obtain second portion Tanshinone I I A extract, two parts merge, and obtain the tanshinone ⅡA extract;

Step 2, getting step 1 Radix Salviae Miltiorrhizae alcohol extraction medicinal residues adds 2-15 and doubly measures 10-90% alcohol extraction 1-8 hour, filter, last medicinal residues add 2-15 and doubly measure 10-90% alcohol extraction 1-8 hour, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 1-3, standing, filter, filtrate is crossed macroporous resin (S-8, AB-8 or D101), wash with water successively, the 5-20% ethanol elution, finally use the 40-90% ethanol elution, collect the 40-90% ethanol elution, concentrated, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds the ethanol of 2-12 times of 50-80%, reflux, extract, 2 times, each 1-3 hour, filter, and extracting solution concentrating under reduced pressure, drying, obtain Radix Notoginseng extract;

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 2, the aqueous alkali that adds for the first time 2-12 doubly to measure PH7-8 decocts 1-3 hour, adds for the second time 2-12 times of water gaging to decoct 0.5-2 hour, obtains extracting solution, extracting solution is concentrated, add ethanol to medicinal liquid and measure 50%-80%, standing 12-36 hour, separation of supernatant containing alcohol, reclaim ethanol, receive cream to pol 55%-80%, obtain Radix Salviae Miltiorrhizae extractum;

Step 5, the tanshinone ⅡA extract of step 1, the salvianolic acid B extract, Radix Notoginseng extract is suspended in the PEG-6000 of pre-thawing successively; Radix Salviae Miltiorrhizae extractum adds the water mix homogeneously; The said two devices mix homogeneously; Add again Borneolum Syntheticum, mix; The material temperature is 60-100 ℃; The temperature of liquid paraffin is 0-10 ℃, makes drop pill;

Wherein described in step 1 and 2, alcohol is selected from: methanol, ethanol, normal propyl alcohol, isopropyl alcohol, n-butyl alcohol or isobutanol alcohol, described extracting mode is selected from: reflux, extract,, warm macerating extraction, merceration extraction, percolation extraction, supersound extraction, dynamic countercurrent extraction or microwave extraction.

Step 1, Radix Salviae Miltiorrhizae adds 2-10 and doubly measures 80%～100% alcohol reflux 1-5 hour, filters, and medicinal residues are doubly measured alcohol with 2-10, and reflux, extract, 1-5 hour filters, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.2-1 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; Macroporous resin on filtrate (polar resin S-8, low pole Resin A B-8 or non-polar resin D101), the 60-85% ethanol elution, eluent discards, use again the 85-100% ethanol elution, collect eluent, be concentrated into containing alcohol amount 65-85%, standing, filter, the precipitation drying under reduced pressure, obtain second portion Tanshinone I I A extract, two parts merge, and obtain Tanshinone I I A extract;

Step 2, getting step 1 Radix Salviae Miltiorrhizae alcohol extraction medicinal residues adds 2-10 and doubly measures 30-70% alcohol reflux 1-6 hour, filter, last medicinal residues add 2-10 and doubly measure 30-70% alcohol reflux 1-6 hour, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to liquid PH value 1-3, standing, filter, filtrate is crossed macroporous resin (S-8, AB-8 or D101), wash with water successively, the 10-20% ethanol elution, finally use the 40-70% ethanol elution, collect the 40-70% ethanol elution, concentrated, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds the ethanol of 4-10 times of 50-80%, reflux, extract, 2 times, each 1-3 hour, filter, and extracting solution concentrating under reduced pressure, drying, obtain Radix Notoginseng extract;

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 2, add 6-12 doubly to measure PH7-8 for the first time, and the 0.2-0.6% sodium bicarbonate solution decocts 1-3 hour, add for the second time 6-12 times of water gaging to decoct 0.5-2 hour, obtain extracting solution, extracting solution is concentrated, adds ethanol to medicinal liquid and measures 50%-75% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 55%-75%, obtains Radix Salviae Miltiorrhizae extractum;

Step 5, the tanshinone ⅡA extract of step 1, the salvianolic acid B extract, Radix Notoginseng extract is suspended in the PEG-6000 of pre-thawing successively; Radix Salviae Miltiorrhizae extractum adds the water mix homogeneously; The said two devices mix homogeneously; Add again Borneolum Syntheticum, mix; The material temperature is 60-100 ℃; The temperature of liquid paraffin is 0-10 ℃, makes drop pill.

The particularly preferred preparation method of drop pill of the present invention, step is:

Step 1, Radix Salviae Miltiorrhizae adds 3-5 and doubly measures 95% alcohol reflux 2 times, and each 1-2 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.2-0.6 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; Macroporous resin AB-8 on filtrate, 70% ethanol elution, eluent discards, then uses 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract;

Step 2, get step 1 Radix Salviae Miltiorrhizae alcohol extraction medicinal residues, adds the alcohol reflux 2 times that 4-6 doubly measures 40-60%, each 1-2hr, filter, filtrate is concentrated into without alcohol, lets cool to below 10 ℃, add dilute hydrochloric acid and regulate pH value 1.8-2.0, more than the standing 12hr of cold preservation, filter upper AB-8 macroporous resin eluting, first wash with water, eluent discards; With 15% ethanol, wash again,, eluent discards; Finally with 50% ethanol, wash, collect 50% ethanol elution, concentrating under reduced pressure, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds doubly 70% ethanol of 6-8, reflux, extract, 2 times, each 1-2 hour, filter, and extracting solution concentrating under reduced pressure, drying, obtain Radix Notoginseng extract;

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 2, the aqueous alkali that adds for the first time 6-12 doubly to measure PH7-8 decocts 1-3 hour, adds for the second time 8 times of water gagings to decoct 1 hour, obtains extracting solution, extracting solution is concentrated, add ethanol to medicinal liquid and measure 60%-70% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 70%-75%, obtain Radix Salviae Miltiorrhizae extractum;

Step 5, the tanshinone ⅡA extract of step 1, the salvianolic acid B extract, Radix Notoginseng extract is suspended in the PEG-6000 of pre-thawing successively; Radix Salviae Miltiorrhizae extractum adds the water mix homogeneously; The said two devices mix homogeneously; Add again Borneolum Syntheticum, mix; The material temperature is 60-100 ℃; The temperature of liquid paraffin is 5-10 ℃, makes drop pill.

The present invention also comprises the application of medicinal dropping ball in the medicine of the cardiovascular disease such as a kind of resisting coronary heart disease of preparation, angina pectoris by treatment coronary heart disease of the present invention.

The present invention is more more superior than prior art because the change of technique causes the present invention to treat the medicinal dropping ball of coronary heart disease.

Below data declaration the present invention treats the beneficial effect of the medicinal dropping ball of coronary heart disease by experiment.

The medicinal dropping ball that adds treatment coronary heart disease of the present invention before Myocytes Anoxia, the intracellular calcium fluorescence intensity obviously descends, and proves that the medicinal dropping ball for the treatment of coronary heart disease has antagonism myocardial cell calcium overload, the effect of protecting myocardial cell.

After myocardial ischemia-reperfusion, in tissue, high-energy phosphate compound obviously reduces, and the lipid peroxide contents showed increased, cause reperfusion injury.Add the medicinal dropping ball for the treatment of coronary heart disease during pre-perfusion and during postischemic reperfusion before ischemia, in tissue, high-energy phosphate compound is all higher than ischemic reperfusion notes group, two groups of adenosine triphosphate (ATP), cardiac muscular tissue's gland nucleoside total amount (TAN) all approach normal level, and LPO levels is lower than ischemic reperfusion notes group.This result proves, the medicinal dropping ball for the treatment of coronary heart disease before ischemia during pre-perfusion during with postischemic reperfusion all can be by increasing the cardiac energy deposit, the generation of inhibition lipid peroxide and protecting myocardial cell.

Cardiomyocyte cell death is damage type the most serious in myocardial ischemia-reperfusion, comprises necrocytosis and apoptosis.The apoptotic regulation and control that expressed by several genes.Myocardial infarction rear section myocardial cell apoptosis, probably relevant with Scavenging Oxygen Free Radical with the cell calcium overload due to ischemia.The medicinal dropping ball for the treatment of coronary heart disease can obviously reduce apoptosis of cardiac muscle quantity after myocardial ischemia-reperfusion, dwindles the myocardial infarct size of ischemia-reperfusion rat, significantly alleviates the pathology damage degree, and stronger with the medicinal dropping ball dosage increasing effect for the treatment of coronary heart disease.Carrying out the anoxia experiment in the myocardial cell of cultivating shows, the medicinal dropping ball intervention for the treatment of coronary heart disease can obviously be lowered apoptotic proteins Fas and be expressed, slight upregulation of apoptosis Profilin Bcl-2 expresses, and further the medicinal dropping ball of demonstration treatment coronary heart disease has certain influence to the protein expression of apoptosis-related genes in cell.

The medicinal dropping ball for the treatment of coronary heart disease improves blood capillary circulation albumin outside leakage and mast cell degranulation is one of important symbol of microcirculation disturbance.The medicinal dropping ball induced by endotoxin for the treatment of coronary heart disease and the improvement effect of the caused microcirculation disturbance of ischemia-reperfusion, the Rat Mesenteric Microcirculation obstacle that the medicinal dropping ball of confirmation treatment coronary heart disease causes ischemia-reperfusion improves significantly, and shows: 1. can suppress the early stage albumin outside leakage of ischemia that Radix Salviae Miltiorrhizae can not play a role; 2. effectively suppress mast cell degranulation; 3. suppress the generation of vascular endothelial cell and leukocyte oxide; 4. suppress sticking of leukocyte and vascular endothelial cell; 5. also influential to the interaction of the cell adhesion molecules after ischemia-reperfusion, peroxidating and leukocyte and endotheliocyte.

The medicinal dropping ball for the treatment of coronary heart disease has more obviously impact to patient's bulbar Conjunctiva Microcirculation, thrombelastogram, patient's arteriole vessel diameter broadening after medication, and fine vein blood vessel narrow diameter, arteriole blood flow rate and blood flow have more significantly to be increased.

Medicinal dropping ball antioxidation, antiinflammatory, the protection blood vessel endothelium for the treatment of coronary heart disease, the balance between the formation of inhibition atherosclerotic plaque and neointimal hyperplasia vascular endothelial cell associated media nitric oxide (NO) and vasoconstrictive factor endothelin-1 (ET-1) etc. is maintaining the stability of blood vessel.After damage, the medicinal dropping ball for the treatment of coronary heart disease of variable concentrations all obviously alleviates human vascular endothelial damage due to LPS, illustrate that the medicinal dropping ball for the treatment of coronary heart disease has obvious protective effect to vascular endothelial cell.Variable concentrations is treated to the medicinal dropping ball of coronary heart disease for the culture fluid before and after the Hydroperoxide injury modeling, discovery generates at the medicinal dropping ball energy balance NO of prevention and treatment group treatment coronary heart disease, significantly increase cytoactive, endothelial injury due to hydrogen peroxide is had to obvious antagonism.

Atherosclerotic lesion is that a kind of protectiveness inflammation to local damage-fiber proliferative is responded.In the Mottling formation process, lipidosis is most important factor.The medicinal dropping ball treatment for the treatment of coronary heart disease can reduce T-CHOL (TC) and low density lipoprotein, LDL (LDL) cholesterol levels, high density lipoprotein increasing (HDL) cholesterol levels.Ma Xiao waits quietly observing the medicinal dropping ball for the treatment of coronary heart disease to damaging the impact of interior film healing situation with similar method modeling, find that the postoperative NO concentration of medicinal dropping ball group for the treatment of coronary heart disease is apparently higher than matched group, the neointimal hyperplasia degree obviously alleviates than matched group, new intima medium vessels smooth muscle cell and fibrous tissue obviously reduce than matched group, therefore think that the medicinal dropping ball for the treatment of coronary heart disease can promote to damage the reparation of inner membrance, reduces neointimal hyperplasia.

The medicinal dropping ball for the treatment of coronary heart disease increases coronary flow, improves blood flow and uprises lipidemia and can cause vascular endothelial injury, come off, platelet adhesion reaction and gathering.The medicinal dropping ball for the treatment of coronary heart disease can significantly increase the rat coronary flow but not increase heart rate, contributes to improve the supply of heart oxygen and nutrient substance.The medicinal dropping ball for the treatment of coronary heart disease obviously reduces rabbit anteserum TC, triglyceride (TG), LDL, very low density lipoprotein (VLDL) (VLDL) concentration and TC/HDL ratio, and HDL concentration obviously raises.

The medicinal dropping ball for the treatment of coronary heart disease suppresses platelet adhesion reaction and gathering Endothelial dysfunction, endothelium integrity destruction can trigger a series of molecules and biochemical reaction is stagnated blood flow, and blood vessel wall reparation, inhibition or antagonism platelet adhesion reaction and gathering are to prevent thrombotic key link.Medicinal dropping ball D-dimer and the gathering for the treatment of coronary heart disease are to play a role from multiple location, stage construction and many target spots.

The medicinal dropping ball for the treatment of coronary heart disease all has obvious inhibitory action to adenosine diphosphate (ADP) (ADP), thrombin and collagen-induced rat platelet aggregation, and is dose-dependence.The medicinal dropping ball for the treatment of coronary heart disease can suppress high fat and feed dog platelet overactivity.The specificity molecular marker of plasma A membrane granulosa protein-140 (6MP-140) while being platelet activation.Before treatment, treatment group and matched group GMP-140 level are apparently higher than the Healthy People group, after the medicinal dropping ball associating aspirin for treatment for the treatment of coronary heart disease, treatment group angina pectoris symptom, electrocardiogram ischemia improve, blood plasma GMP-140 level is than the remarkable reduction of matched group (singly taking aspirin), with the Healthy People group without significant difference.To the aspirin resistance person, the medicinal dropping ball of add-on coronary heart disease can obviously improve the aspirin resistance state, reduces platelet aggregation rate, and total effective rate is 85%, and the average range of decrease is 51.57%.

Because the medicinal dropping ball for the treatment of coronary heart disease has above-mentioned multiple target effect, it is to all kinds coronary heart disease, comprises that the perioperative treatment etc. of stable or unstable angina pectoris, myocardial infarction, heart failure, silent ischemia and intervention all has remarkable result.

Following experimental data is for illustrating effect of the present invention: the medicine that experimental drug is the embodiment of the present invention 1.

The comparison of medicinal dropping ball aspect the angina pectoris treatment effect of table 1 treatment coronary heart disease

Group	n	Produce effects	Effectively	Invalid	Increase the weight of	Total effective rate/%
							Treatment group	51	19	29	3	0	94.1①
Matched group	51	8	28	14	1	70.6

Annotate: 1. with matched group, compare, analyze U=3.039, P<0.01 through Radit.

The comparison of medicinal dropping ball aspect ECG curative effect of table 2 treatment coronary heart disease

Group	n	Produce effects	Effectively	Invalid	Increase the weight of	Total effective rate/%
							Treatment group	51	12	29	20	0	60.8①
Matched group	51	3	16	30	2	37.3

Annotate: 1. with matched group, compare, analyze U=2.685, P<0.01 through Radit.

Before and after the medicinal dropping ball treatment of table 3 treatment coronary heart disease, hemorheology index changes (x ± s)

The medicinal dropping ball medication left ventricular contractile function of table 4 treatment coronary heart disease is (x ± s) relatively

Annotate: 1. before and after medication, compare P>0.05; 2. compare P<0.05 before and after medication.

That the present invention adopts the modern pharmacy technology to develop is efficient, quick-acting, the pure TCM Dripping Pills of multiple-effect, prevent and treat the coronary heart disease determined curative effect, and can improve the blood capillary circulation, to the diabetes microvascular complication, comprise that diabetic renal papillary necrosis and diabetic nephropathy all have good preventive and therapeutic effect.

The invention has the advantages that: this process route can be realized industrialization, stable and controllable for quality.Related tests shows, the present invention is higher than prior art curative effect, and product purity is high, good absorbing, and steady quality, the purposes novelty, preparation method technique is simple, easy to operate, with low cost simultaneously, is applicable to suitability for industrialized production.

The specific embodiment

Below with embodiment, illustrate the present invention, embodiment is for the ease of understanding the present invention, and the claim do not limited the present invention in any way and core content.

Embodiment 1

The preparation of the medicine for the treatment of coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 82.87%, Radix Notoginseng 16.21%, Borneolum Syntheticum 0.92%;

Step 1, Radix Salviae Miltiorrhizae adds 5 times of amount 95% ethanol, and reflux, extract, 2 hours, filter, 3 times of amount 95% ethanol for medicinal residues, reflux, extract, 1 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.4-0.5 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; AB-8 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion Tanshinone I I A extract, and two parts merge, and obtain the tanshinone ⅡA extract

Step 2, get step 1 alcohol extraction medicinal residues, adds 3 times of amount 20% alcohol reflux 1hr, filter, medicinal residues add 4 times of amount 50% alcohol reflux 1hr, filter, filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 1.8-2.0, more than the standing 12hr of cold preservation, filter, upper AB-8 macroporous resin, first wash with water, and eluent discards; With 15% ethanol, wash, eluent discards again; Finally with 50% ethanol, wash, collect 50% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds the ethanol of 8 times of amounts 70%, and reflux, extract, 2 hours is filtered, and medicinal residues add 6 times of amount 70% ethanol, reflux, extract, 1 hour, extracting liquid filtering is concentrated into clarification, and decompression recycling ethanol, drying, obtain Radix Notoginseng extract;

Step 4, the medicinal residues of salvianolic acid B after the ethanol extraction of step 2, add for the first time the aqueous alkali of 8 times of amount PH7-8 to decoct 2 hours, add for the second time 8 times of water gagings to decoct 1 hour, obtain extracting solution, extracting solution is concentrated, add ethanol to medicinal liquid and measure 60%-70% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 70%-75%, obtain Radix Salviae Miltiorrhizae extractum;

Step 5, the tanshinone ⅡA extract obtained after said extracted, the salvianolic acid B extract, Radix Notoginseng extract is suspended in the PEG-6000 of pre-thawing successively; Radix Salviae Miltiorrhizae extractum adds the water mix homogeneously; The said two devices mix homogeneously; Add again Borneolum Syntheticum, mix; The material temperature is 60-100 ℃; The temperature of liquid paraffin is 5-10 ℃, makes drop pill.

The preparation of the medicine of embodiment 2 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 19.8%, Radix Notoginseng 78.2%, Borneolum Syntheticum 2%;

Step 1, Radix Salviae Miltiorrhizae adds 2 times of amount 95% ethanol, and reflux, extract, 1 hour, filter, 20 times of amount 95% ethanol for medicinal residues, reflux, extract, 1 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.4-0.6 times of medical material amount, standing, filters; Precipitation is dry, obtains first's tanshinone ⅡA extract; AB-8 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 2, add for the first time the aqueous alkali of 2 times of amount PH7-8 to decoct 1 hour, add for the second time 2 times of water gagings to decoct 0.5 hour, obtain extracting solution, extracting solution is concentrated, add ethanol to medicinal liquid and measure 60%-70% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 70%-75%, obtain Radix Salviae Miltiorrhizae extractum;

The preparation of embodiment 3 FUFANG DANSHEN DIWAN, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 97%, Radix Notoginseng 2%, Borneolum Syntheticum 1%,

Step 1, Radix Salviae Miltiorrhizae adds 2 times of amount 95% ethanol, and reflux, extract, 1 hour, filter, 20 times of amount 95% ethanol for medicinal residues, reflux, extract, 10 hours, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.2 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; AB-8 macroporous resin on filtrate, 50% ethanol elution, eluent discards, and continues to use 85% ethanol elution, collect eluent, be concentrated into containing alcohol amount 65%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract;

Step 2, get step 1 alcohol extraction medicinal residues, adds 8 times of amount 60% alcohol reflux 4hr, filter, medicinal residues add 4 times of amount 50% alcohol reflux 1hr, filter, filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 1.8-2.0, more than the standing 12hr of cold preservation, filter, upper AB-8 macroporous resin, first wash with water, and eluent discards; With 15% ethanol, wash, eluent discards again; Finally with 50% ethanol, wash, collect 50% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 2, add for the first time the aqueous alkali of 6 times of amount PH7-8 to decoct 3 hours, add for the second time 6 times of water gagings to decoct 2 hours, obtain extracting solution, extracting solution is concentrated, add ethanol to medicinal liquid and measure 60%-70% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 70%-75%, obtain Radix Salviae Miltiorrhizae extractum;

The preparation of the medicine of embodiment 4 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 95%, Radix Notoginseng 2%, Borneolum Syntheticum 3%,

Step 1, Radix Salviae Miltiorrhizae adds 15 times of amount 95% ethanol, and reflux, extract, 3 hours, filter, 15 times of amount 95% ethanol for medicinal residues, reflux, extract, 10 hours, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 5 times of medical material amounts, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; S-8 macroporous resin on filtrate, 85% ethanol elution, eluent discards, and continues to use 100% ethanol elution, collect eluent, be concentrated into containing alcohol amount 85%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract;

Step 2, get step 1 alcohol extraction medicinal residues, adds 4 times of amount 50% ethanol ultrasonic extraction 2hr, filter, medicinal residues add 4 times of amount 50% ethanol ultrasonic extraction 1hr, filter, filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 1.8-2.0, more than the standing 12hr of cold preservation, pulp board filters, upper AB-8 macroporous resin, first wash with water, and eluent discards; With 15% ethanol, wash, eluent discards again; Finally with 50% ethanol, wash, collect 50% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 4, Radix Salviae Miltiorrhizae decoction dregs after the ethanol extraction of step 2, add for the first time the aqueous alkali of 8 times of amount PH7-8 to decoct 2 hours, add for the second time 6 times of water gagings to decoct 1 hour, obtain extracting solution, extracting solution is concentrated, add ethanol to medicinal liquid and measure 60%-70% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 70%-75%, obtain Radix Salviae Miltiorrhizae extractum;

The preparation of the medicine of embodiment 5 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 19.8%, Radix Notoginseng 80%, Borneolum Syntheticum 0.2%,

Step 1, Radix Salviae Miltiorrhizae adds 2 times of amount 95% ethanol, and reflux, extract, 3 hours, filter, 15 times of amount 95% ethanol for medicinal residues, reflux, extract, 2 hours, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 1 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; D101 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract;

Step 2, get step 1 alcohol extraction medicinal residues, adds 4 times of amount 50% ethanol dynamic countercurrent extraction 2hr, filter, medicinal residues add 4 times of amount 50% ethanol dynamic countercurrent extraction 1hr, filter, filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 1.8-2.0, more than the standing 12hr of cold preservation, filter, upper AB-8 macroporous resin, first wash with water, and eluent discards; With 15% ethanol, wash, eluent discards again; Finally with 50% ethanol, wash, collect 50% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 2, add for the first time the aqueous alkali of 8 times of amount PH7-8 to decoct 2 hours, add for the second time 6 times of water gagings to decoct 1 hour, obtain extracting solution, extracting solution is concentrated, add ethanol to medicinal liquid and measure 60%-70% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 70%-75%, obtain Radix Salviae Miltiorrhizae extractum;

Step 5, the Tanshinone I I A extract obtained after said extracted, the salvianolic acid B extract, Radix Notoginseng extract is suspended in the PEG-6000 of pre-thawing successively; Radix Salviae Miltiorrhizae extractum adds the water mix homogeneously; The said two devices mix homogeneously; Add again Borneolum Syntheticum, mix; The material temperature is 60-100 ℃; The temperature of liquid paraffin is 5-10 ℃, makes drop pill.

The preparation of the medicine of embodiment 6 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 20.8%, Radix Notoginseng 78.6%, Borneolum Syntheticum 0.6%,

Step 1, Radix Salviae Miltiorrhizae adds 10 times of amount 95% ethanol, and reflux, extract, 3 hours, filter, 2 times of amount 95% ethanol for medicinal residues, reflux, extract, 2 hours, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 1 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; D101 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract;

Step 2, get step 1 alcohol extraction medicinal residues, adds 4 times of amount 50% alcohol reflux 2hr, filter, medicinal residues add 4 times of amount 50% alcohol reflux 1hr, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 1.0-4.0, more than the standing 12hr of cold preservation, upper AB-8 macroporous resin, first wash with water, eluent discards; With 15% ethanol, wash, eluent discards again; Finally with 50% ethanol, wash, collect 50% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 4, the salvianolic acid B medicinal residues after the ethanol extraction of step 2, add the aqueous alkali of 8 times of amount PH7-8 to decoct for the first time 2 hours, adds for the second time 6 times of water gagings to decoct 1 hour, obtains extracting solution, and extracting solution is concentrated, adds ethanol to medicinal liquid and contains alcohol amount 60%-70%; Standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 70%-75%, obtains Radix Salviae Miltiorrhizae extractum;

The preparation of the medicine of embodiment 7 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 29.8%, Radix Notoginseng 68.6%, Borneolum Syntheticum 1.6%,

Step 1, Radix Salviae Miltiorrhizae adds 10 times of amount 60% ethanol, and reflux, extract, 3 hours, filter, 8 times of amount 60% ethanol for medicinal residues, reflux, extract, 2 hours, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 1 times of medical material amount, standing, filters; Precipitation is dry, obtains first's tanshinone ⅡA extract; D101 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract;

Step 2, get step 1 alcohol extraction medicinal residues, adds 2 times of amount 10% ethanol warm macerating and extract 2hr, filter, medicinal residues add 4 times of amount 90% ethanol warm macerating and extract 1hr, filter, filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 1.8-2.0, more than the standing 12hr of cold preservation, filter, on filtrate, the AB-8 macroporous resin, first wash with water, and eluent discards; With 15% ethanol, wash, eluent discards again; Finally with 50% ethanol, wash, collect 50% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

The preparation of the medicine of embodiment 8 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 86.8%, Radix Notoginseng 12.6%, Borneolum Syntheticum 0.6%,

Step 1, Radix Salviae Miltiorrhizae adds 10 times of amount 100% ethanol, and reflux, extract, 3 hours, filter, 8 times of amount 60% ethanol for medicinal residues, reflux, extract, 2 hours, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 1 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; D101 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract;

Step 2, get step 1 alcohol extraction medicinal residues, adds 4 times of amount 50% ethanol ultrasonic extraction 2hr, filter, medicinal residues add 4 times of amount 50% ethanol ultrasonic extraction 1hr, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 2.0-4.0, more than the standing 12hr of cold preservation, upper AB-8 macroporous resin, first wash with water, eluent discards; With 15% ethanol, wash, eluent discards again; Finally with 50% ethanol, wash, collect 50% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 5, the tanshinone ⅡA extract obtained after said extracted, salvianolic acid B extract, Radix Notoginseng extract, Radix Salviae Miltiorrhizae extractum, Borneolum Syntheticum, get appropriate cane sugar powder, essence adds appropriate water, makes oral liquid.

The preparation of the medicine of embodiment 9 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 85.8%, Radix Notoginseng 12.6%, Borneolum Syntheticum 1.6%,

Step 1, Radix Salviae Miltiorrhizae adds 10 times of amount 95% ethanol, and reflux, extract, 10 hours, filter, 8 times of amount 95% ethanol for medicinal residues, reflux, extract, 1 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 1 times of medical material amount, standing, filters; Precipitation is dry, obtains first's tanshinone ⅡA extract; D101 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract;

Step 2, get step 1 alcohol extraction medicinal residues, adds 4 times of amount 50% ethanol ultrasonic extraction 2hr, filter, medicinal residues add 4 times of amount 50% ethanol ultrasonic extraction 1hr, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 1.0-2.0, more than the standing 12hr of cold preservation, upper S-8 macroporous resin, first wash with water, eluent discards; With 15% ethanol, wash, eluent discards again; Finally with 50% ethanol, wash, collect 50% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds the ethanol of 6 times of amounts 70%, and reflux, extract, 1 hour is filtered, and medicinal residues add 8 times of amount 70% ethanol, reflux, extract, 2 hours, extracting liquid filtering is concentrated into clarification, and decompression recycling ethanol, drying, obtain Radix Notoginseng extract;

Step 5, the tanshinone ⅡA extract obtained after said extracted, the salvianolic acid B extract, Radix Notoginseng extract is suspended in the PEG-6000 of pre-thawing successively; Radix Salviae Miltiorrhizae extractum adds the water mix homogeneously; The said two devices mix homogeneously; Add again Borneolum Syntheticum, mix; The material temperature is 80-100 ℃; The temperature of liquid paraffin is 5-10 ℃, makes drop pill.

The preparation of the medicine of embodiment 10 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 74.8%, Radix Notoginseng 23.6%, Borneolum Syntheticum 1.6%,

Step 1, Radix Salviae Miltiorrhizae adds 10 times of amount 95% ethanol, and reflux, extract, 3 hours, filter, 8 times of amount 95% ethanol for medicinal residues, reflux, extract, 2 hours, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 1 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; D101 macroporous resin on filtrate, 60% ethanol elution, eluent discards, and continues to use 85% ethanol elution, collect eluent, be concentrated into containing alcohol amount 65%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract;

Step 2, get step 1 alcohol extraction medicinal residues, adds 4 times of amount 50% ethanol dynamic countercurrent extraction 2hr, filter, medicinal residues add 4 times of amount 50% ethanol dynamic countercurrent extraction 1hr, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 2.0-4.0, more than the standing 12hr of cold preservation, upper AB-8 macroporous resin, first wash with water, eluent discards; With 15% ethanol, wash, eluent discards again; Finally with 50% ethanol, wash, collect 50% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 5, the tanshinone ⅡA extract obtained after said extracted, salvianolic acid B extract, Radix Notoginseng extract, Radix Salviae Miltiorrhizae extractum, Borneolum Syntheticum, add appropriate amount of starch, mixes, and tabletting, make tablet.

The preparation of the medicine of embodiment 11 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 58.8%, Radix Notoginseng 40.6%, Borneolum Syntheticum 0.6%,

Step 1, Radix Salviae Miltiorrhizae adds 4 times of amount 95% ethanol, and reflux, extract, 1 hour, filter, 5 times of amount 95% ethanol for medicinal residues, reflux, extract, 2 hours, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.2 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; D101 macroporous resin on filtrate, 60% ethanol elution, eluent discards, and continues to use 85% ethanol elution, collect eluent, be concentrated into containing alcohol amount 65%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract;

Step 2, get step 1 alcohol extraction medicinal residues, adds 4 times of amount 50% methanol dynamic countercurrent extraction 2hr, filter, medicinal residues add 4 times of amount 50% methanol dynamic countercurrent extraction 1hr, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 2.0-3.0, more than the standing 12hr of cold preservation, upper AB-8 macroporous resin, first wash with water, eluent discards; With 15% ethanol, wash, eluent discards again; Finally with 50% ethanol, wash, collect 50% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds the ethanol of 4 times of amounts 70%, and reflux, extract, 1 hour is filtered, and medicinal residues add 8 times of amount 70% ethanol, reflux, extract, 2 hours, extracting liquid filtering is concentrated into clarification, and decompression recycling ethanol, drying, obtain Radix Notoginseng extract;

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 2, add for the first time the aqueous alkali of 2 times of amount PH7-8 to decoct 2 hours, add for the second time 2 times of water gagings to decoct 1 hour, obtain extracting solution, extracting solution is concentrated, add ethanol to medicinal liquid and measure 60%-70% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 70%-75%, obtain Radix Salviae Miltiorrhizae extractum;

The preparation of the medicine of embodiment 12 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 28%, Radix Notoginseng 70%, Borneolum Syntheticum 2%;

Step 1, Radix Salviae Miltiorrhizae adds 10 times of amount 65% methanol, and reflux, extract, 1 hour, filter, 5 times of amount 95% methanol for medicinal residues, reflux, extract, 5 hours, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.8 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; D101 macroporous resin on filtrate, 85% ethanol elution, eluent discards, and continues to use 100% ethanol elution, collect eluent, be concentrated into containing alcohol amount 85%, standing, filter, the precipitation drying under reduced pressure, obtain second portion Tanshinone I I A extract, and two parts merge, and obtain the tanshinone ⅡA extract;

Step 2, get step 1 alcohol extraction medicinal residues, adds 4 times of amount 50% methanol dynamic countercurrent extraction 2hr, filter, medicinal residues add 4 times of amount 50% methanol dynamic countercurrent extraction 1hr, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 2.0-3.0, more than the standing 12hr of cold preservation, upper AB-8 macroporous resin, first wash with water, eluent discards; With 10% ethanol, wash, eluent discards again; Finally with 45% ethanol, wash, collect 45% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds the ethanol of 4 times of amounts 80%, and reflux, extract, 1 hour is filtered, and medicinal residues add 10 times of amount 70% ethanol, reflux, extract, 3 hours, extracting liquid filtering is concentrated into clarification, and decompression recycling ethanol, drying, obtain Radix Notoginseng extract;

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 2, add for the first time the aqueous alkali of 12 times of amount PH7-8 to decoct 2 hours, add for the second time 12 times of water gagings to decoct 1 hour, obtain extracting solution, extracting solution is concentrated, add ethanol to medicinal liquid and measure 60%-70% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 70%-75%, obtain Radix Salviae Miltiorrhizae extractum;

Step 5, the Tanshinone I I A extract obtained after said extracted, the salvianolic acid B extract, Radix Notoginseng extract is suspended in the PEG-6000 of pre-thawing successively; Radix Salviae Miltiorrhizae extractum adds the water mix homogeneously; The said two devices mix homogeneously; Add again Borneolum Syntheticum, mix; The material temperature is 80 ℃; The temperature of liquid paraffin is 8 ℃, makes drop pill.

The preparation of the medicine of embodiment 13 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 85%, Radix Notoginseng 12%, Borneolum Syntheticum 3%;

Step 1, Radix Salviae Miltiorrhizae adds 5 times of amount 95% normal propyl alcohols, and reflux, extract, 2 hours, filter, 3 times of amount 95% normal propyl alcohols for medicinal residues, reflux, extract, 1 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.4-0.5 times of medical material amount, standing, filters; Precipitation is dry, obtains first's tanshinone ⅡA extract; AB-8 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion Tanshinone I I A extract, and two parts merge, and obtain the tanshinone ⅡA extract

Step 2, get step 1 alcohol extraction medicinal residues, adds 4 times of amount 50% n-butyl alcohol dynamic countercurrent extraction 2hr, filter, medicinal residues add 4 times of amount 50% n-butyl alcohol dynamic countercurrent extraction 1hr, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 2.0-3.0, more than the standing 12hr of cold preservation, upper AB-8 macroporous resin, first wash with water, eluent discards; With 13% ethanol, wash, eluent discards again; Finally with 65% ethanol, wash, collect 65% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds the ethanol of 4 times 80%, reflux, extract, 3 times, each 3 hours, to filter, extracting solution concentrating under reduced pressure, drying, obtain Radix Notoginseng extract;

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 2, add for the first time the aqueous alkali of 7 times of amount PH7-8 to decoct 2 hours, add for the second time 6 times of water gagings to decoct 1 hour, obtain extracting solution, extracting solution is concentrated, add ethanol to medicinal liquid and measure 50%-80% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 50%, obtain Radix Salviae Miltiorrhizae extractum;

The preparation of the medicine of embodiment 14 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 50%, Radix Notoginseng 48%, Borneolum Syntheticum 2%;

Step 1, Radix Salviae Miltiorrhizae adds 5 times of amount 95% isopropyl alcohols, and reflux, extract, 2 hours, filter, 3 times of amount 95% isopropyl alcohols for medicinal residues, reflux, extract, 1 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.4-0.5 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; AB-8 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion Tanshinone I I A extract, and two parts merge, and obtain the tanshinone ⅡA extract

Step 2, get step 1 alcohol extraction medicinal residues, adds 4 times of amount 50% methanol dynamic countercurrent extraction 2hr, filter, medicinal residues add 4 times of amount 50% methanol dynamic countercurrent extraction 1hr, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 2.0-3.0, more than the standing 12hr of cold preservation, upper D101 macroporous resin, first wash with water, eluent discards; With 10% ethanol, wash, eluent discards again; Finally with 70% ethanol, wash, collect 70% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds the ethanol of 10 times 80%, reflux, extract, 1 time, each 1 hour, to filter, extracting solution concentrating under reduced pressure, drying, obtain Radix Notoginseng extract;

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 2, add for the first time the aqueous alkali of 7 times of amount PH7-10 to decoct 2 hours, add for the second time 6 times of water gagings to decoct 1 hour, obtain extracting solution, extracting solution is concentrated, add ethanol to medicinal liquid and measure 50%-80% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 90%, obtain Radix Salviae Miltiorrhizae extractum;

Step 5, the tanshinone ⅡA extract obtained after said extracted, salvianolic acid B extract, Radix Notoginseng extract, Radix Salviae Miltiorrhizae extractum, Borneolum Syntheticum, add appropriate dextrin, mucialga of arabic gummy to mix, and granulates; Incapsulate, make capsule.

The preparation of the medicine of embodiment 15 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 90%, Radix Notoginseng 8%, Borneolum Syntheticum 2%;

Step 1, Radix Salviae Miltiorrhizae adds 5 times of amount 95% n-butyl alcohol, and reflux, extract, 2 hours, filter, 3 times of amount 95% n-butyl alcohol for medicinal residues, reflux, extract, 1 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.4-0.5 times of medical material amount, standing, filters; Precipitation is dry, obtains first's tanshinone ⅡA extract; AB-8 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion Tanshinone I I A extract, and two parts merge, and obtain the tanshinone ⅡA extract

Step 2, get step 1 alcohol extraction medicinal residues, adds 4 times of amount 50% isobutanol dynamic countercurrent extraction 2hr, filter, medicinal residues add 4 times of amount 50% isobutanol dynamic countercurrent extraction 1hr, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 2.0-3.0, more than the standing 12hr of cold preservation, upper AB-8 macroporous resin, first wash with water, eluent discards; With 13% ethanol, wash, eluent discards again; Finally with 65% ethanol, wash, collect 65% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds the ethanol of 6 times 75%, reflux, extract, 2 times, each 2 hours, to filter, extracting solution concentrating under reduced pressure, drying, obtain Radix Notoginseng extract;

The preparation of the medicine of embodiment 16 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 82%, Radix Notoginseng 17%, Borneolum Syntheticum 1%;

Step 1, Radix Salviae Miltiorrhizae adds 5 times of amount 95% isobutanol, and reflux, extract, 2 hours, filter, 3 times of amount 95% isobutanol for medicinal residues, reflux, extract, 1 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.4-0.5 times of medical material amount, standing, filters; Precipitation is dry, obtains first's tanshinone ⅡA extract; AB-8 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract

Step 2, get step 1 alcohol extraction medicinal residues, adds 4 times of amount 50% n-butyl alcohol dynamic countercurrent extraction 2hr, filter, medicinal residues add 4 times of amount 50% n-butyl alcohol dynamic countercurrent extraction 1hr, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 2.0-3.0, more than the standing 12hr of cold preservation, upper AB-8 macroporous resin, first wash with water, eluent discards; With 13% ethanol, wash, eluent discards again; Finally with 70% ethanol, wash, collect 70% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds the ethanol of 7 times 80%, reflux, extract, 3 times, each 1.5 hours, to filter, extracting solution concentrating under reduced pressure, drying, obtain Radix Notoginseng extract;

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 2, add for the first time the aqueous alkali of 6 times of amount PH7-10 to decoct 2 hours, add for the second time 9 times of water gagings to decoct 2 hours, obtain extracting solution, extracting solution is concentrated, add ethanol to medicinal liquid and measure 50%-80% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 90%, obtain Salvia miltiorrhiza and Panax notoginseng extractum;

Step 5, the tanshinone ⅡA extract obtained after said extracted, salvianolic acid B extract, Radix Notoginseng extract, Radix Salviae Miltiorrhizae extractum, Borneolum Syntheticum, add crystallite cellulose, sodium carboxymethyl cellulose, gelatinslurry mixes; Granulate, the granulation agent.

The preparation of the medicine of embodiment 17 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 82.87%, Radix Notoginseng 16.21%, Borneolum Syntheticum 0.92%;

Step 1, Radix Salviae Miltiorrhizae adds 5 times of amount 95% ethanol, and reflux, extract, 2 hours, filter, 3 times of amount 95% ethanol for medicinal residues, reflux, extract, 1 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 2 times of medical material amounts, standing, filters; Precipitation is dry, obtains first's tanshinone ⅡA extract; S-8 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract

Step 2, get step 1 Radix Salviae Miltiorrhizae alcohol extraction medicinal residues and add 2 times of amounts, 10% isopropyl alcohol warm macerating extraction 0.5 hour, filters, last medicinal residues add 2 times of pure warm macerating of amount and extract 10 hours, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to liquid PH value 1-4, standing, filter, filtrate is crossed macroporous resin D101, washes with water successively, 10% ethanol elution, 60% ethanol elution, collect 60% ethanol elution, concentrated, drying, obtain the salvianolic acid B extract;

Step 3, Radix Notoginseng adds the ethanol of 50 times 50%, reflux, extract, 3 times, each 3 hours, to filter, extracting solution concentrating under reduced pressure, drying, obtain Radix Notoginseng extract;

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 5, add 8 times of amount PH7-8 for the first time, and 0.2% sodium bicarbonate solution decocts 2 hours, add for the second time 8 times of water gagings to decoct 1 hour, obtain extracting solution, extracting solution is concentrated, adds ethanol to medicinal liquid and measures 60%-70% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 70%-75%, obtains Radix Salviae Miltiorrhizae extractum;

Step 5, the tanshinone ⅡA extract obtained after said extracted, salvianolic acid B extract, Radix Notoginseng extract, Radix Salviae Miltiorrhizae extractum, Borneolum Syntheticum, add appropriate amount of starch, Mel mix homogeneously; Make pill.

The preparation of the medicine of embodiment 18 treatment coronary heart disease, each component consists of in the weight percent of crude drug effective ingredient: Radix Salviae Miltiorrhizae 82.87%, Radix Notoginseng 16.21%, Borneolum Syntheticum 0.92%;

Step 1, Radix Salviae Miltiorrhizae adds 5 times of amount 95% ethanol, and reflux, extract, 2 hours, filter, 3 times of amount 95% ethanol for medicinal residues, reflux, extract, 1 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 3 times of medical material amounts, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; AB-8 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion tanshinone ⅡA extract, and two parts merge, and obtain the tanshinone ⅡA extract

Step 2, get step 1 Radix Salviae Miltiorrhizae alcohol extraction medicinal residues and add 20 times of amount 10% normal propyl alcohol reflux, extract, 10 hours, filters, last medicinal residues add 20 times of amounts alcohol extraction 1.5 hours, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to liquid PH value 1-4, standing, filter, filtrate is crossed macroporous resin S-8, washes with water successively, 10% ethanol elution, 60% ethanol elution, collect 60% ethanol elution, concentrated, drying, obtain the salvianolic acid B extract;

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 2, add 8 times of amount PH7-8 for the first time, and 0.6% sodium bicarbonate solution decocts 2 hours, add for the second time 8 times of water gagings to decoct 1 hour, obtain extracting solution, extracting solution is concentrated, adds ethanol to medicinal liquid and measures 60%-70% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 70%-75%, obtains Radix Salviae Miltiorrhizae extractum;

Step 5, the Tanshinone I I A extract, salvianolic acid B extract, Radix Notoginseng extract, Radix Salviae Miltiorrhizae extractum, the Borneolum Syntheticum that after said extracted, obtain, add appropriate mannitol, magnesium oxide, calcium carbonate to mix; Tabletting, make tablet.

Claims

1. a medicine for the treatment of coronary heart disease, described medicine is prepared from by the crude drug of following percentage by weight, Radix Salviae Miltiorrhizae 19.8%-97%, Radix Notoginseng 2%-80%, Borneolum Syntheticum 0.2%-3%, described preparation is first crude drug to be prepared into to active constituents of medicine through following steps, further be prepared into again medicine

Step 1, Radix Salviae Miltiorrhizae adds 2-20 and doubly measures 60%～100% alcohol extraction 1-10 hour, filters, and medicinal residues are doubly measured 60%～100% alcohol with 2-20, extract 1-10 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.2-5 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; Macroporous resin AB-8 on filtrate, the 50-85% ethanol elution, eluent discards, then uses the 85-100% ethanol elution, collect eluent, be concentrated into containing alcohol amount 65-85%, standing, filter, the precipitation drying under reduced pressure, obtain second portion Tanshinone I I A extract, two parts merge, and obtain Tanshinone I I A extract;

Step 2, get step 1 Radix Salviae Miltiorrhizae alcohol extraction medicinal residues and add 2-20 and doubly measure 10-100% alcohol extraction 0.5-10 hour, filters, last medicinal residues add 2-20 and doubly measure alcohol extraction 0.5-10 hour, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to liquid PH value 1-4, standing, filter, filtrate is crossed macropore Resin A B-8, washes with water successively, low concentration alcohol eluting, high concentration alcohol eluting, collect the high concentration alcohol eluen, concentrated, drying, obtain the salvianolic acid B extract;

Step 5, by Tanshinone I I A extract obtained above, salvianolic acid B extract, Radix Notoginseng extract, Radix Salviae Miltiorrhizae extractum mixes with adjuvant with Borneolum Syntheticum, adds any or more than one adjuvants, the 1-6 that the adjuvant addition is active component doubly, makes any oral formulations;

Wherein described in step 1 and 2, alcohol is selected from: methanol, ethanol, normal propyl alcohol, isopropyl alcohol, n-butyl alcohol or isobutanol, described extracting mode is selected from: reflux, extract,, warm macerating extraction, merceration extraction, percolation extraction, supersound extraction, dynamic countercurrent extraction or microwave extraction

2. the medicine of the treatment coronary heart disease of claim 1, be drop pill, it is characterized in that, preparation process is:

Step 1, Radix Salviae Miltiorrhizae adds 2-15 and doubly measures 60%～100% alcohol extraction 1-8 hour, filters, and medicinal residues are doubly measured 60%～100% alcohol with 2-15, extract 1-8 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.2-3 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; Macroporous resin AB-8 on filtrate, the 50-85% ethanol elution, eluent discards, then uses the 85-100% ethanol elution, collect eluent, be concentrated into containing alcohol amount 65-85%, standing, filter, the precipitation drying under reduced pressure, obtain second portion Tanshinone I I A extract, two parts merge, and obtain Tanshinone I I A extract;

Step 2, get step 1 Radix Salviae Miltiorrhizae alcohol extraction medicinal residues and add 2-15 and doubly measure 10-90% alcohol extraction 1-8 hour, filters, last medicinal residues add 2-15 and doubly measure 10-90% alcohol extraction 1-8 hour, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 1-3, standing, filter, filtrate is crossed macropore Resin A B-8, washes with water successively, the 5-20% ethanol elution, finally use the 40-90% ethanol elution, collects the 40-90% ethanol elution, concentrated, drying, obtain the salvianolic acid B extract;

Step 5, the Tanshinone I I A extract of step 1, the salvianolic acid B extract, Radix Notoginseng extract is suspended in the PEG-6000 of pre-thawing successively; Radix Salviae Miltiorrhizae extractum adds the water mix homogeneously; The said two devices mix homogeneously; Add again Borneolum Syntheticum, mix; The material temperature is 60-100 ℃; The temperature of liquid paraffin is 0-10 ℃, makes drop pill;

Wherein described in step 1 and 2, alcohol is selected from: methanol, ethanol, normal propyl alcohol, isopropyl alcohol, n-butyl alcohol or isobutanol, described extracting mode is selected from: reflux, extract,, warm macerating extraction, merceration extraction, percolation extraction, supersound extraction, dynamic countercurrent extraction or microwave extraction.

3. the medicine of the treatment coronary heart disease of claim 1: it is characterized in that, preparation process is:

Step 1, Radix Salviae Miltiorrhizae adds 2-10 and doubly measures 80%～100% alcohol reflux 1-5 hour, filters, and medicinal residues are doubly measured alcohol with 2-10, and reflux, extract, 1-5 hour filters, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.2-1 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; Macroporous resin AB-8 on filtrate, the 60-85% ethanol elution, eluent discards, then uses the 85-100% ethanol elution, collect eluent, be concentrated into containing alcohol amount 65-85%, standing, filter, the precipitation drying under reduced pressure, obtain second portion Tanshinone I I A extract, two parts merge, and obtain Tanshinone I I A extract;

Step 2, getting step 1 Radix Salviae Miltiorrhizae alcohol extraction medicinal residues adds 2-10 and doubly measures 30-70% alcohol reflux 1-6 hour, filter, last medicinal residues add 2-10 and doubly measure 30-70% alcohol reflux 1-6 hour, filter, and filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to liquid PH value 1-3, standing, filter, filtrate is crossed macropore Resin A B-8, wash with water successively, the 10-20% ethanol elution, finally use the 40-70% ethanol elution, collect the 40-70% ethanol elution, concentrated, drying, obtain the salvianolic acid B extract;

Step 5, the Tanshinone I I A extract of step 1, the salvianolic acid B extract, Radix Notoginseng extract is suspended in the PEG-6000 of pre-thawing successively; Radix Salviae Miltiorrhizae extractum adds the water mix homogeneously; The said two devices mix homogeneously; Add again Borneolum Syntheticum, mix; The material temperature is 60-100 ℃; The temperature of liquid paraffin is 0-10 ℃, makes drop pill.

4. the medicine of the treatment coronary heart disease of claim 3: it is characterized in that, preparation process is:

Step 1, Radix Salviae Miltiorrhizae adds 3-5 and doubly measures 95% alcohol reflux 2 times, and each 1-2 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.2-0.6 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; Macroporous resin AB-8 on filtrate, 70% ethanol elution, eluent discards, then uses 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion Tanshinone I I A extract, and two parts merge, and obtain Tanshinone I I A extract;

Step 4, salvianolic acid B medicinal residues after the ethanol extraction of step 2, add 6-12 doubly to measure PH7-8 for the first time, and the 0.2-0.6% sodium bicarbonate solution decocts 1-3 hour, add for the second time 8 times of water gagings to decoct 1 hour, obtain extracting solution, extracting solution is concentrated, adds ethanol to medicinal liquid and measures 60%-70% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 70%-75%, obtains Radix Salviae Miltiorrhizae extractum;

Step 5, the Tanshinone I I A extract of step 1, the salvianolic acid B extract, Radix Notoginseng extract is suspended in the PEG-6000 of pre-thawing successively; Radix Salviae Miltiorrhizae extractum adds the water mix homogeneously; The said two devices mix homogeneously; Add again Borneolum Syntheticum, mix; The material temperature is 60-100 ℃; The temperature of liquid paraffin is 5-10 ℃, makes drop pill.

5. the medicine of the treatment coronary heart disease of claim 4: it is characterized in that, preparation process is:

Step 1, Radix Salviae Miltiorrhizae adds 5 times of amount 95% ethanol, and reflux, extract, 2 hours, filter, 3 times of amount 95% ethanol for medicinal residues, reflux, extract, 1 hour, filter, and medicinal residues extract for salvianolic acid B, and filtrate is concentrated into 0.4-0.5 times of medical material amount, standing, filters; Precipitation is dry, obtains the Tanshinone I I A of first extract; AB-8 macroporous resin on filtrate, 70% ethanol elution, eluent discards, and continues to use 95% ethanol elution, collect eluent, be concentrated into containing alcohol amount 80%, standing, filter, the precipitation drying under reduced pressure, obtain second portion Tanshinone I I A extract, and two parts merge, and obtain Tanshinone I I A extract

Step 2, get step 1 alcohol extraction medicinal residues, adds 3 times of amount 20% alcohol reflux 1hr, filter, medicinal residues add 4 times of amount 50% alcohol reflux 1hr, filter, filtrate is concentrated into without alcohol, let cool to below 10 ℃, add dilute hydrochloric acid to medicinal liquid pH value 1.8-2.0, more than the standing 12hr of cold preservation, pulp board filters, upper AB-8 macroporous resin, first wash with water, and eluent discards; With 15% ethanol, wash, eluent discards again; Finally with 50% ethanol, wash, collect 50% ethanol washing liquid, concentrating under reduced pressure below 60 ℃, drying, obtain the salvianolic acid B extract;

Step 4, the medicinal residues of salvianolic acid B after the ethanol extraction of step 2, add 8 times of amount PH7-8 for the first time, and 0.45% sodium bicarbonate solution decocts 2 hours, add for the second time 8 times of water gagings to decoct 1 hour, obtain extracting solution, extracting solution is concentrated, adds ethanol to medicinal liquid and measures 60%-70% containing alcohol, standing more than 12 hours, separation of supernatant, reclaim ethanol, receive cream to pol 70%-75%, obtains Radix Salviae Miltiorrhizae extractum;

6. the medicine of the arbitrary described treatment coronary heart disease of claim 1-5: it is characterized in that, step 5 is, the Tanshinone I I A extract obtained after step 1 is extracted, the salvianolic acid B extract that step 2 obtains after extracting, the Radix Notoginseng extract that step 3 obtains after extracting, the Radix Salviae Miltiorrhizae extractum that step 4 obtains after extracting mixes with Borneolum Syntheticum and adjuvant, add any or more than one adjuvants, described adjuvant is selected from fat-soluble substrate and water-soluble base, wherein fat-soluble substrate is selected from: stearic acid, glyceryl monostearate, insect wax, Cera Flava, paraffin, hydrogenated vegetable oil, semi-synthetic fatty acid ester, water-soluble base is selected from Polyethylene Glycol, polyoxyethylene monostearate, poloxamer, sodium stearate, glycerin gelatine, xylitol and composites of starch, erythritol.

7. the medicine of the treatment coronary heart disease of claim 6: it is characterized in that, wherein the auxiliary ratio of step 5 medicine is 1: 3.0.

8. the medicine of the treatment coronary heart disease of claim 7: it is characterized in that, wherein said Polyethylene Glycol is PEG-4000.

9. the application of the medicine of the treatment coronary heart disease of claim 1 in the medicine of a kind of resisting coronary heart disease of preparation, angina pectoris cardiovascular disease.