CN102027112B - 核酸重组方法 - Google Patents

核酸重组方法 Download PDF

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Publication number
CN102027112B
CN102027112B CN2009801137719A CN200980113771A CN102027112B CN 102027112 B CN102027112 B CN 102027112B CN 2009801137719 A CN2009801137719 A CN 2009801137719A CN 200980113771 A CN200980113771 A CN 200980113771A CN 102027112 B CN102027112 B CN 102027112B
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nucleic acid
sequence
target nucleic
strand displacement
stranded
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Expired - Fee Related
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CN2009801137719A
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Chinese (zh)
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CN102027112A (zh
Inventor
马尔切诺·马雷斯卡
阿克塞尔·斯特凡·埃勒尔
付军
菲利普·马丁·赛贝特
阿迪安·弗朗西斯·斯图尔特
张佑明
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Gene Bridges GmbH
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Gene Bridges GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN2009801137719A 2008-02-20 2009-02-20 核酸重组方法 Expired - Fee Related CN102027112B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0803109.8 2008-02-20
GBGB0803109.8A GB0803109D0 (en) 2008-02-20 2008-02-20 Method of nucleic acid recombination
PCT/IB2009/000488 WO2009104094A2 (en) 2008-02-20 2009-02-20 Method of nucleic acid recombination

Publications (2)

Publication Number Publication Date
CN102027112A CN102027112A (zh) 2011-04-20
CN102027112B true CN102027112B (zh) 2013-11-06

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CN2009801137719A Expired - Fee Related CN102027112B (zh) 2008-02-20 2009-02-20 核酸重组方法

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US (1) US8728820B2 (enExample)
EP (1) EP2255001B1 (enExample)
JP (1) JP5703031B2 (enExample)
CN (1) CN102027112B (enExample)
AU (1) AU2009215361A1 (enExample)
CA (1) CA2715871A1 (enExample)
GB (1) GB0803109D0 (enExample)
WO (1) WO2009104094A2 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011058555A1 (en) * 2009-11-12 2011-05-19 Yeda Research And Development Co. Ltd. A method of editing dna in a cell and constructs capable of same
GB201009732D0 (en) * 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
EP2768607B1 (en) 2011-09-26 2021-08-18 Thermo Fisher Scientific GENEART GmbH Multiwell plate for high efficiency, small volume nucleic acid synthesis
US10563240B2 (en) * 2013-03-14 2020-02-18 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
EP3557262B1 (en) 2014-12-09 2022-08-10 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
RU2707137C2 (ru) 2014-12-19 2019-11-22 Регенерон Фармасьютикалз, Инк. Способы и композиции для нацеленной генетической модификации посредством одноэтапного множественного нацеливания
WO2016196887A1 (en) * 2015-06-03 2016-12-08 Board Of Regents Of The University Of Nebraska Dna editing using single-stranded dna
CN106011162A (zh) * 2016-01-27 2016-10-12 浙江大学 大肠杆菌细胞内原位进化目标蛋白质的新方法
WO2017174753A1 (en) 2016-04-06 2017-10-12 Gene Bridges Gmbh Adenoviral vectors
JP6899564B2 (ja) * 2016-08-12 2021-07-07 Ｎｅｘｕｓｐｉｒａｌ株式会社 ゲノム編集方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
ATE242320T1 (de) * 1997-12-05 2003-06-15 Europ Lab Molekularbiolog Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems
US6355412B1 (en) * 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
AU8337701A (en) * 2000-08-14 2002-02-25 Us Health Enhanced homologous recombination mediated by lambda recombination proteins
US20040029129A1 (en) * 2001-10-25 2004-02-12 Liangsu Wang Identification of essential genes in microorganisms

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides;COURT D.L.;《PROC NATL ACAD SCI USA》;20011231;第98卷(第12期);6742-6746 *
Red 同源重组技术研究进展;韩聪;《中国生物工程杂志》;20031231;第23卷(第12期);17-21 *

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Publication number Publication date
US20110165630A1 (en) 2011-07-07
GB0803109D0 (en) 2008-03-26
CN102027112A (zh) 2011-04-20
AU2009215361A1 (en) 2009-08-27
WO2009104094A2 (en) 2009-08-27
EP2255001A2 (en) 2010-12-01
EP2255001B1 (en) 2015-03-25
WO2009104094A3 (en) 2009-12-17
US8728820B2 (en) 2014-05-20
JP2011512153A (ja) 2011-04-21
CA2715871A1 (en) 2009-08-27
JP5703031B2 (ja) 2015-04-15

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Granted publication date: 20131106

Termination date: 20200220