AU2009215361A1 - Method of nucleic acid recombination - Google Patents

Method of nucleic acid recombination Download PDF

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Publication number
AU2009215361A1
AU2009215361A1 AU2009215361A AU2009215361A AU2009215361A1 AU 2009215361 A1 AU2009215361 A1 AU 2009215361A1 AU 2009215361 A AU2009215361 A AU 2009215361A AU 2009215361 A AU2009215361 A AU 2009215361A AU 2009215361 A1 AU2009215361 A1 AU 2009215361A1
Authority
AU
Australia
Prior art keywords
nucleic acid
single stranded
replacement
region
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2009215361A
Other languages
English (en)
Inventor
Axel Steffen Erler
Jun Fu
Marcello Maresca
Philipp Martin Seibert
Adrian Francis Stewart
Youming Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gene Bridges GmbH
Original Assignee
Gene Bridges GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gene Bridges GmbH filed Critical Gene Bridges GmbH
Publication of AU2009215361A1 publication Critical patent/AU2009215361A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU2009215361A 2008-02-20 2009-02-20 Method of nucleic acid recombination Abandoned AU2009215361A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0803109.8 2008-02-20
GBGB0803109.8A GB0803109D0 (en) 2008-02-20 2008-02-20 Method of nucleic acid recombination
PCT/IB2009/000488 WO2009104094A2 (en) 2008-02-20 2009-02-20 Method of nucleic acid recombination

Publications (1)

Publication Number Publication Date
AU2009215361A1 true AU2009215361A1 (en) 2009-08-27

Family

ID=39272012

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2009215361A Abandoned AU2009215361A1 (en) 2008-02-20 2009-02-20 Method of nucleic acid recombination

Country Status (8)

Country Link
US (1) US8728820B2 (enExample)
EP (1) EP2255001B1 (enExample)
JP (1) JP5703031B2 (enExample)
CN (1) CN102027112B (enExample)
AU (1) AU2009215361A1 (enExample)
CA (1) CA2715871A1 (enExample)
GB (1) GB0803109D0 (enExample)
WO (1) WO2009104094A2 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011058555A1 (en) * 2009-11-12 2011-05-19 Yeda Research And Development Co. Ltd. A method of editing dna in a cell and constructs capable of same
GB201009732D0 (en) 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
WO2014153188A2 (en) * 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US12595500B2 (en) 2011-09-26 2026-04-07 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
LT2768607T (lt) 2011-09-26 2021-12-27 Thermo Fisher Scientific Geneart Gmbh Daugiašulininė plokštelė didelio efektyvumo, mažo tūrio nukleorūgščių sintezei atlikti
WO2016094512A1 (en) 2014-12-09 2016-06-16 yLIFE TECHNOLOGIES CORPORATION High efficiency, small volume nucleic acid synthesis
MX388784B (es) 2014-12-19 2025-03-20 Regeneron Pharma Métodos y composiciones para la modificación genética dirigida a través de direccionamiento múltiple de una sola etapa.
CN108368502B (zh) * 2015-06-03 2022-03-18 内布拉斯加大学董事委员会 使用单链dna的dna编辑
CN106011162A (zh) * 2016-01-27 2016-10-12 浙江大学 大肠杆菌细胞内原位进化目标蛋白质的新方法
EP3440213A1 (en) 2016-04-06 2019-02-13 Gene Bridges GmbH Adenoviral vectors
US20190284581A1 (en) * 2016-08-12 2019-09-19 Nexuspiral Inc. Genome editing method

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
ES2337027T3 (es) * 1997-12-05 2010-04-20 Europaisches Laboratorium Fur Molekularbiologie (Embl) Nuevo metodo de clonacion de dna basado en el sistema de recombinacion rece-rect de e. coli.
US6355412B1 (en) * 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
ES2394877T3 (es) * 2000-08-14 2013-02-06 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Recombinación homóloga mejorada mediada por proteínas de recombinación de lambda
US20040029129A1 (en) * 2001-10-25 2004-02-12 Liangsu Wang Identification of essential genes in microorganisms

Also Published As

Publication number Publication date
JP5703031B2 (ja) 2015-04-15
WO2009104094A2 (en) 2009-08-27
WO2009104094A3 (en) 2009-12-17
CA2715871A1 (en) 2009-08-27
CN102027112B (zh) 2013-11-06
US8728820B2 (en) 2014-05-20
EP2255001B1 (en) 2015-03-25
US20110165630A1 (en) 2011-07-07
EP2255001A2 (en) 2010-12-01
JP2011512153A (ja) 2011-04-21
CN102027112A (zh) 2011-04-20
GB0803109D0 (en) 2008-03-26

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Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application