CN102018222B - Method for preparing radix puerariae dietary fibers by using environmental-friendly multi-step enzyme method - Google Patents
Method for preparing radix puerariae dietary fibers by using environmental-friendly multi-step enzyme method Download PDFInfo
- Publication number
- CN102018222B CN102018222B CN201010548044XA CN201010548044A CN102018222B CN 102018222 B CN102018222 B CN 102018222B CN 201010548044X A CN201010548044X A CN 201010548044XA CN 201010548044 A CN201010548044 A CN 201010548044A CN 102018222 B CN102018222 B CN 102018222B
- Authority
- CN
- China
- Prior art keywords
- root
- kudzu vine
- radix puerariae
- dietary fiber
- amylase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for preparing radix puerariae dietary fibers by using an environmental-friendly multi-step enzyme method, which comprises the following steps: removing starches, proteins and fats contained in radix puerariae residues by using the multi-step enzyme method; and then sequentially carrying out enzyme destruction, decoloring, cleaning, drying and crushing and the like on the obtained radix puerariae residues so as to obtain high-quality radix puerariae dietary fiber powder. The method is characterized in that the starches in the radix puerariae residues are effectively removed by composite amylases, thereby being environmental friendly and improving the product quality; the fats in radix puerariae residues are removed by lipases, thereby reducing the fat content in the dietary fibers and facilitating the function of the fiber product; the proteins in the radix puerariae residues are removed by proteases, thereby facilitating the storage of the product; and the purity of the radix puerariae dietary fiber prepared from the radix puerariae residues is more than 80 percent. By using the method, the problems of low fat removal rate, complicated production process, long consumed time and low efficiency existing in the process of extracting the radix puerariae dietary fibers from radix puerariae residues are solved; and because the multi-step enzyme method is adopted in the invention, amounts of waste water and organic solvents are not produced in the whole production process, thereby effectively solving the pollution problem existing in the production of the radix puerariae dietary fibers.
Description
Technical field
The present invention relates to a kind of food processing technology field, be specifically related to the dietary fiber process technology, particularly a kind of environmental protection using multi-step enzyme method is produced the method for root of kudzu vine dietary fiber.
Background technology
At present, how the domestic Pueraria lobota Products Development of being engaged in is to produce products such as kudzuvine root starch, root of kudzu vine grain, kudzu root beverage, flavones medicinal extract, after having extracted active ingredient; Remaining root of kudzu vine slag is taken as rubbish topples over; As feed, paste fails to make full use of resource to fewer companies with it.The method of taking of producing of dietary fiber is an acid-base method at present.It mainly is to utilize sour starch-splitting that acid-base method is produced dietary fiber; Utilize the alkali solubilising protein; But strong solvent (acid, alkali etc.) is handled dissolved the losing of cellulose caused almost 100% water-soluble fibre, 50% one 60% hemicellulose and 10% one 30%; And acid-base method can not be removed the fat in the raw material effectively, and the waste water that it produced can pollute environment.
Summary of the invention
The object of the present invention is to provide a kind of multistep enzymatic isolation method extracts root of kudzu vine dietary fiber from root of kudzu vine slag method of passing through, thereby improve the comprehensive utilization ratio of the root of kudzu vine.
For realizing above-mentioned purpose, the technical scheme that the present invention adopted is:
A kind of environmental protection using multi-step enzyme method is produced the method for root of kudzu vine dietary fiber; It is characterized in that: root of kudzu vine slag is fully pulverized the back mix with water; Be added into composite alpha-amylase, protease and lipase successively and handle, fractional hydrolysis non-dietary fiber component wherein correspondingly, the hydrolysis temperature when wherein said composite alpha-amylase is handled is 50-90 ℃; Hydrolysis time is 1-12 hour, and the part by weight of composite alpha-amylase and root of kudzu vine slag is 1: 300-500; Hydrolysis temperature during described Protease Treatment is 50-90 ℃, and hydrolysis time is 1-12 hour, and the part by weight of protease and root of kudzu vine slag is 1: 150-250; Hydrolysis temperature when described lipase is handled is 50-90 ℃; Hydrolysis time is 1-12 hour; The part by weight of lipase and root of kudzu vine slag is 1: 250-350, obtain root of kudzu vine dietary fiber bullion after go out behind the three step enzymolysis enzyme, the washing, and the hydrogenperoxide steam generator with 2-10% decolours then; Through cleaning, oven dry, pulverising step, make final products root of kudzu vine dietary fiber again.
In the above-mentioned preparation method:
The weight content of insoluble diedairy fiber is that the weight content of 50-70%, moisture is 8-15% in the described root of kudzu vine slag.
Described root of kudzu vine slag and water are by 1: the part by weight of 8-10 mixes.
Described composite alpha-amylase is AMS and beta amylase, and the part by weight of the two is 1: 1-5.
Go out behind the enzymolysis process of enzyme, washing of described three steps is to be warming up to 97 ± 2 ℃, and insulation 1-2 hour filters to stop the enzyme effect then, uses the water washing filter residue.
The 2-3 that described hydrogenperoxide steam generator consumption is an enzymolysis product weight doubly.
Described bleaching time is 1-2 hour.
Described cleaning step is triplicate at least, the 3rd time cleaning solution of a batch materials through filter centrifugal after, can be as the first pass slurry of another batch materials.
Temperature is controlled at 70-90 ℃ in the described baking step.
The present invention's beneficial effect compared with prior art is:
The present invention utilizes composite alpha-amylase effectively to remove the starch in the root of kudzu vine slag, helps environmental protection and improves the quality of products; Utilize lipase to remove fat in the root of kudzu vine slag, reduced the fat content in the dietary fiber, help the storage function of product; Utilize protease to remove protein in the root of kudzu vine slag, help the storage of product.Utilize hydrogenperoxide steam generator that dietary fiber is decoloured, improved the dietary fiber color, improved the attractive in appearance of product.The root of kudzu vine dietary fiber purity of from root of kudzu vine slag, producing through the present invention can reach more than 80%.It is low to the invention solves from root of kudzu vine slag when extracting dietary fiber the fat clearance, complex manufacturing, length consuming time, the problem that benefit is low; And because the present invention adopts the multistep enzymatic isolation method, make whole process of production can not produce a large amount of waste water and organic solvent, efficiently solve the pollution problem in the production of root of kudzu vine dietary fiber.The present invention has improved the value and the economic benefit of the root of kudzu vine, satisfies the demand of people to dietary fiber, and the product of gained can be used as dietary fiber enriched dose of numerous food.
The specific embodiment
Following examples only are used to set forth the present invention, and protection scope of the present invention is not only to be confined to following examples.Content disclosed by the invention and scope that each parameter is got more than the those of ordinary skill foundation of said technical field all can realize the object of the invention.The technological process that following examples adopt is: root of kudzu vine slag is blended with pulverizer; Add a certain proportion of water then; Add a certain proportion of composite alpha-amylase; Under suitable soak time, temperature, carry out enzymolysis, under suitable condition, carry out protease hydrolyzed and lipase enzymolysis to filter residue again, the water washing filter residue is to neutral then.Behind the enzyme that goes out, collect filter residue, decolouring is cleaned, and oven dry is pulverized, and obtains root of kudzu vine dietary fiber finished product.
Embodiment 1
Pulverize, add water: raw material root of kudzu vine slag is pulverized, and with the mixed of water by 1: 10 (w/w);
Composite alpha-amylase is handled: adopt composite alpha-amylase to remove starch.Composite alpha-amylase is AMS and beta amylase, and the two ratio is 1: 3 (w/w).Hydrolysis temperature is 70 ± 2 ℃, and the reaction time is 1.5 hours.The ratio of composite alpha-amylase and raw material is 1: 500 (w/w);
Protease Treatment: adopt protease to remove protein.Hydrolysis temperature is 50 ± 2 ℃, and the reaction time is 1 hour, and the ratio of protease and raw material is 1: 250 (w/w);
Lipase is handled: adopt lipase to remove fat.Hydrolysis temperature is 55 ± 2 ℃, and the reaction time is 2 hours, and the ratio of lipase and raw material is 1: 350 (w/w);
Go out enzyme, washing: be warming up to 97 ± 2 ℃, and be incubated 1 hour, filter then, use the water washing filter residue to stop the enzyme effect;
Decolouring: the hydrogenperoxide steam generator decolouring of adding 10%, the consumption of hydrogen peroxide is 2 times of enzymolysis product quality, decolouring is 1 hour under the room temperature;
Clean: use the clear water washed twice, the consumption that at every turn washs clear water is 2 times of weight of material.Second time cleaning solution of one batch materials through filter centrifugal after, can be as the first pass slurry of another batch materials;
Oven dry: under 80 ℃ condition, dried by the fire 8 hours, product moisture is qualified less than 10%;
Pulverize: the product after will drying is pulverized, and obtains final products root of kudzu vine food fibre powder.
Embodiment 2
Pulverize, add water: raw material root of kudzu vine slag is pulverized, and with the mixed of water by 1: 10 (w/w);
Composite alpha-amylase is handled: adopt composite alpha-amylase to remove starch.Composite alpha-amylase is AMS and beta amylase, and the two ratio is 1: 3 (w/w).Hydrolysis temperature is 70 ± 2 ℃, and the reaction time is 1.5 hours.The ratio of composite alpha-amylase and raw material is 1: 400 (w/w);
Protease Treatment: adopt protease to remove protein.Hydrolysis temperature is 50 ± 2 ℃, and the reaction time is 1 hour, and the ratio of protease and raw material is 1: 150 (w/w);
Lipase is handled: adopt lipase to remove fat.Hydrolysis temperature is 55 ± 2 ℃, and the reaction time is 2 hours, and the ratio of lipase and raw material is 1: 300 (w/w);
Go out enzyme, washing: be warming up to 97 ± 2 ℃, and be incubated 1 hour, filter then, use the water washing filter residue to stop the enzyme effect;
Decolouring: the hydrogenperoxide steam generator decolouring of adding 10%, the consumption of hydrogen peroxide is 2 times of enzymolysis product quality, decolouring is 1.5 hours under the room temperature;
Clean: use the clear water washed twice, the consumption that at every turn washs clear water is 2 times of weight of material.Second time cleaning solution of one batch materials through filter centrifugal after, can be as the first pass slurry of another batch materials;
Oven dry: under 90 ℃ condition, dried by the fire 7 hours, product moisture is qualified less than 10%;
Pulverize: the product after will drying is pulverized, and obtains final products root of kudzu vine food fibre powder.
Embodiment 3
Pulverize, add water: raw material root of kudzu vine slag is pulverized, and with the mixed of water by 1: 10 (w/w);
Composite alpha-amylase is handled: adopt composite alpha-amylase to remove starch.Composite alpha-amylase is AMS and beta amylase, and the two ratio is 1: 3 (w/w).Hydrolysis temperature is 70 ± 2 ℃, and the reaction time is 1.5 hours.The ratio of composite alpha-amylase and raw material is 1: 300 (w/w);
Protease Treatment: adopt protease to remove protein.Hydrolysis temperature is 50 ± 2 ℃, and the reaction time is 1 hour, and the ratio of protease and raw material is 1: 200 (w/w);
Lipase is handled: adopt lipase to remove fat.Hydrolysis temperature is 55 ± 2 ℃, and the reaction time is 2 hours, and the ratio of lipase and raw material is 1: 250 (w/w);
Go out enzyme, washing: be warming up to 97 ± 2 ℃, and be incubated 1 hour, filter then, use the water washing filter residue to stop the enzyme effect;
Decolouring: the hydrogenperoxide steam generator decolouring of adding 10%, the consumption of hydrogen peroxide is 2 times of enzymolysis product quality, decolouring is 1 hour under the room temperature;
Clean: use the clear water washed twice, the consumption that at every turn washs clear water is 2 times of weight of material.Second time cleaning solution of one batch materials through filter centrifugal after, can be as the first pass slurry of another batch materials;
Oven dry: under 80 ℃ condition, dried by the fire 8 hours, product moisture is qualified less than 10%;
Pulverize: the product after will drying is pulverized, and obtains final products root of kudzu vine food fibre powder.
The beneficial effect of the embodiment of the invention can detect through following method and draw:
Starch: the mensuration of starch in the GB/T5009.9-2003 food
Fat: the mensuration of fat in the GB/T5009.6-2003 food
Protein: protein measuring in the GB/T5009.5-2003 food
Insoluble fiber: the mensuration of insoluble diedairy fiber in the GB/T5009.88-2003 food
Soluble fibre: the mensuration of water-soluble dietary fiber in the AOAC993.19 food
Moisture: atmosphere pressure desiccation
Ash content: calcination method
Test result such as following table:
Raw material, root of kudzu vine diet fiber product composition and index
| Project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Raw material |
| Insoluble diedairy fiber/% | 77.51 | 77.36 | 76.96 | 48.19 |
| Soluble dietary fiber/% | 5.3 | 5.0 | 5.1 | 8.6 |
| Total dietary fiber/% | 82.81 | 82.36 | 82.06 | 56.79 |
| Protein/%, N * 5.70 | 2.31 | 2.55 | 2.64 | 6.78 |
| Starch/% | 5.01 | 5.12 | 5.31 | 15.62 |
| Fat/% | 0.21 | 0.32 | 0.26 | 1.73 |
| Moisture/% | 7.34 | 7.56 | 6.93 | 10.16 |
| Ash content/% | 1.5 | 1.7 | 1.6 | 2.08 |
Claims (1)
1. an environmental protection using multi-step enzyme method is produced the method for root of kudzu vine dietary fiber; It is characterized in that: root of kudzu vine slag is fully pulverized the back mix with water; Be added into composite alpha-amylase, protease and lipase successively and handle, fractional hydrolysis non-dietary fiber component wherein correspondingly, the hydrolysis temperature when wherein said composite alpha-amylase is handled is 50-90 ℃; Hydrolysis time is 1-12 hour; The part by weight of composite alpha-amylase and root of kudzu vine slag is 1: 300-500, described composite alpha-amylase are AMS and beta amylase, and the part by weight of the two is 1: 1-5; Hydrolysis temperature during described Protease Treatment is 50-90 ℃, and hydrolysis time is 1-12 hour, and the part by weight of protease and root of kudzu vine slag is 1: 150-250; Hydrolysis temperature when described lipase is handled is 50-90 ℃, and hydrolysis time is 1-12 hour, and the part by weight of lipase and root of kudzu vine slag is 1: 250-350; Go out behind the enzymolysis enzyme, washing of three steps; The process of enzyme, washing of going out is to be warming up to 97 ± 2 ℃, and insulation 1-2 hour is filtered to stop the enzyme effect then; Obtain root of kudzu vine dietary fiber bullion with the water washing filter residue; With the hydrogenperoxide steam generator decolouring of 2-10%,, make final products root of kudzu vine dietary fiber then again through cleaning, oven dry, pulverising step.
2. a kind of environmental protection using multi-step enzyme method according to claim 1 is produced the method for root of kudzu vine dietary fiber, it is characterized in that: the weight content of insoluble diedairy fiber is that the weight content of 50-70%, moisture is 8-15% in the described root of kudzu vine slag.
3. a kind of environmental protection using multi-step enzyme method according to claim 1 is produced the method for root of kudzu vine dietary fiber, it is characterized in that: described root of kudzu vine slag and water are by 1: the part by weight of 8-10 mixes.
4. a kind of environmental protection using multi-step enzyme method according to claim 1 is produced the method for root of kudzu vine dietary fiber, it is characterized in that: the 2-3 that described hydrogenperoxide steam generator consumption is an enzymolysis product weight doubly.
5. a kind of environmental protection using multi-step enzyme method according to claim 1 is produced the method for root of kudzu vine dietary fiber, it is characterized in that: described bleaching time is 1-2 hour.
6. a kind of environmental protection using multi-step enzyme method according to claim 1 is produced the method for root of kudzu vine dietary fiber; It is characterized in that: described cleaning step is triplicate at least; The 3rd time cleaning solution of one batch materials through filter centrifugal after, can be as the first pass slurry of another batch materials.
7. a kind of environmental protection using multi-step enzyme method according to claim 1 is produced the method for root of kudzu vine dietary fiber, it is characterized in that: temperature is controlled at 70-90 ℃ in the described baking step.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010548044XA CN102018222B (en) | 2010-11-18 | 2010-11-18 | Method for preparing radix puerariae dietary fibers by using environmental-friendly multi-step enzyme method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010548044XA CN102018222B (en) | 2010-11-18 | 2010-11-18 | Method for preparing radix puerariae dietary fibers by using environmental-friendly multi-step enzyme method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102018222A CN102018222A (en) | 2011-04-20 |
| CN102018222B true CN102018222B (en) | 2012-12-12 |
Family
ID=43860322
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010548044XA Expired - Fee Related CN102018222B (en) | 2010-11-18 | 2010-11-18 | Method for preparing radix puerariae dietary fibers by using environmental-friendly multi-step enzyme method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102018222B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107711989A (en) * | 2017-12-08 | 2018-02-23 | 江西省三抗保健品有限公司 | A kind of preparation method of the balanced weight losing meal-replacing biscuit of nutrition |
| CN108308242A (en) * | 2018-05-09 | 2018-07-24 | 河南中医药大学 | A kind of Pueraria lobota fibre biscuit of hypoglycemic defaecation |
| CN110771905A (en) * | 2019-11-18 | 2020-02-11 | 江西中医药大学 | Method for preparing kudzu vine root dietary fiber with good expansibility by enzyme method |
| CN115868639B (en) * | 2022-12-30 | 2023-08-08 | 德兴市宋氏葛业有限公司 | Digestion-aiding kudzuvine root dietary fiber added biscuit and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530674A (en) * | 2009-03-16 | 2009-09-16 | 王艺瑾 | Process for extracting active ingredients of traditional Chinese medicine in unit and combined type and device thereof |
-
2010
- 2010-11-18 CN CN201010548044XA patent/CN102018222B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530674A (en) * | 2009-03-16 | 2009-09-16 | 王艺瑾 | Process for extracting active ingredients of traditional Chinese medicine in unit and combined type and device thereof |
Non-Patent Citations (5)
| Title |
|---|
| 张彬等.酶解法提取甘薯渣中水溶性膳食纤维的研究.《粮油加工》.2008,(第01期), * |
| 苏勇等.葛根膳食纤维对小白鼠免疫功能的影响.《中南林学院学报》.2006,第26卷(第04期), * |
| 苏勇等.葛根膳食纤维对小白鼠降血糖的影响.《食品与机械》.2006,第22卷(第05期), * |
| 钟海雁等.从葛根渣中酶法制备膳食纤维.《作物学报》.2005,第31卷(第12期), * |
| 韩军等.葛根膳食纤维漂白工艺的研究.《食品与机械》.2005,第21卷(第01期), * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102018222A (en) | 2011-04-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101889677B (en) | Method for extracting dietary fiber from tartary buckwheat bran | |
| CN102492665B (en) | Compound enzyme preparation for fermentation of pu'er tea and application | |
| CN101837973B (en) | Preparation method of activated carbon by utilizing corncob hydrolysis residue | |
| CN102187984B (en) | Preparation method for soluble dietary fiber with apple pomace as raw material | |
| CN104223125B (en) | A kind of method preparing potato residues dietary fiber | |
| CN102018222B (en) | Method for preparing radix puerariae dietary fibers by using environmental-friendly multi-step enzyme method | |
| CN105433393A (en) | Method for preparing dietary fiber from purple sweet potato residues used as raw materials | |
| CN102309011A (en) | Preparation method of dietary fiber of corn | |
| CN101912108A (en) | Method for preparing dietary fiber by extracting potato residues | |
| CN103788216B (en) | A kind of method of producing microcrystalline cellulose as raw material taking the full fruit of coconut | |
| CN101278709A (en) | Method for extracting maltose powder, protein hydrolysate powder and cellulose using bran as raw material | |
| CN108484944A (en) | A kind of preparation method of edibility dietary fiber packaging paper membrane | |
| CN101569387B (en) | Method for producing water chestnut sheet jelly | |
| CN101597629B (en) | Method for preparing xyio-oligosacchandes by using ultrasonic to treat agricultural wastes | |
| CN102356881B (en) | Preparation method for high water-holding capacity and water-insolubility corn dietary fiber | |
| CN104223348A (en) | Method by combining biological enzyme and microwave expansion technologies to improve quality of tobacco stems | |
| CN106811497A (en) | The technique that a kind of utilization corncob hydrolysis residue prepares activated carbon | |
| CN102533436A (en) | Method for processing health-care camellia oil | |
| CN103734748A (en) | Method for extracting dietary fiber from laminaria japonica | |
| CN103907938A (en) | Preparation method of jujube fruit residue dietary fiber | |
| CN104341537A (en) | Method for arsenic removal and extraction of nutrient substances in sargassum fusiforme | |
| CN101439995A (en) | Clean production method of seaweed fertilizer | |
| CN102181076B (en) | Method for cleanly preparing starch containing abundant flavones and comprehensively utilizing radix puerariae resource | |
| CN104928341A (en) | Preparation method for ferulic acid combining ultrasonic-assisted enzymolysis and microbial-fermented bran | |
| CN102630887A (en) | Method for extracting dietary fibers from edible vegetables |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121212 Termination date: 20141118 |
|
| EXPY | Termination of patent right or utility model |