CN102007869A - Ectomycorrhizal fungi efficiently symbiotic with pinus koraiensis - Google Patents
Ectomycorrhizal fungi efficiently symbiotic with pinus koraiensis Download PDFInfo
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- CN102007869A CN102007869A CN 201010551671 CN201010551671A CN102007869A CN 102007869 A CN102007869 A CN 102007869A CN 201010551671 CN201010551671 CN 201010551671 CN 201010551671 A CN201010551671 A CN 201010551671A CN 102007869 A CN102007869 A CN 102007869A
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- korean pine
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Abstract
The invention discloses ectomycorrhizal fungi efficiently symbiotic with pinus koraiensis, relates to the ectomycorrhizal fungi, and overcomes the defects that the conventional artificial breeding annual pinus koraiensis seedlings are short, grow slowly and have low biomass, low absorption rate of nitrogen, phosphorus, potassium and other nutrient substances, small stalks, yellow leaves and low survival rate. The ectomycorrhizal fungi efficiently symbiotic with the pinus koraiensis are suillus sp. HDSF2 and collected in China General Microbiological Culture Collection Center (CGMCC) with the collection number of CGMCC No.4175. The suillus sp. HDSF2 can be used for the artificial breeding of the pinus koraiensis.
Description
Technical field
The present invention relates to a strain ectotrophic mycorrhiza fungi.
Background technology
Korean pine has another name called korean pine, belongs to pinaceae plant, belongs to national II level and lays special stress on protecting wild plant.Mainly be distributed in Changbai Mountain, China northeast to the Xiaoxinanlin Mountains one band.Korean pine is precious and ancient seeds, and pine nut has very high nutritive value and value medical health care, and potentiality to be exploited is huge; And as precious economic trees, it is light and soft that Korean pine has material, and not yielding, the corrosion resistant ability is strong, is applicable to building, bridge, sleeper, furniture making etc.Just because of the high economic worth of Korean pine, from Xiaoxinanlin Mountains exploitation in 1948 till now, go through the felling in 56 years, the neutral red pine forest is reduced to 50,000 hectares of less thaies, approximately only remaining 3,000,000 strains of ripe forest from original 1,200,000 hectares.In order to recover the Korean pine virgin forest, preserve the ecological environment, also be the needs of Korean pine industrialization development in the future, the artificial planting Korean pine becomes the task of top priority.
In the Korean pine artificial breeding technique, use the nursery, land for growing field crops to cultivate more at present, perhaps directly utilize beans stubble crop rotation ground to cultivate, also have and use the nutrition earth culture to support.Before Korean pine is grown seedlings, all culture matrixes are all wanted sterilization, this can eliminate the soil-borne disease insect pest, but also eliminated microorganism useful in the soil to plant growing, cause annual Korean pine height of seedling short, absorption of nutrient ingredients rates such as poor growth, biomass are few, nitrogen phosphorus potassium are low, cane is tiny, the leaf look withered and yellow, and survival rate is low.
Summary of the invention
The present invention is short in order to solve the annual Korean pine height of seedling of present Korean pine artificial breeding, absorption of nutrient ingredients rates such as poor growth, biomass are few, nitrogen phosphorus potassium are low, cane is tiny, the leaf look withered and yellow, the defective that survival rate is low, and the ectotrophic mycorrhiza fungi of a strain that provides and the efficient symbiosis of Korean pine.
The ectotrophic mycorrhiza fungi of the present invention and the efficient symbiosis of Korean pine is bolete (Suillus sp.) HDSF2, is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC No.4175.
To large-scale, bacteria cover diameter is 6～12cm, be flat hemispherical, the back center projections, central recessed, smooth, sticking, color is a russet sometimes in the fruit body of bolete (Suillus sp.) HDSF2.Bolete HDSF2 bacterial context is faint yellow; The tube initial colour is light, and the thin out lark in back is hindered thin out aubergine or band brown, straight giving birth to closely prolonging life; The mouth of pipe is less, dihedral, and part is compound, 1～2 every millimeter; Handle length is 6～12cm, thick 1～2.6cm, and nearly cylindricality is omited etc. thick up and down or base portion is thin slightly, no gland point, there is reticulate pattern on the top.Bolete (Suillus sp.) HDSF2 spore is to print yellowish-brown to Chestnut, and the oval or near spindle of spore is level and smooth, is with olive-yellow, and length and width are (8.7～10.4 μ m) * (3.5～4.2 μ m); How bar-shaped pipe flange is colourless to filbert, scattered to clustering, with pipe side utricule, and length and width are (26～83 μ m) * (5.2～6 μ m).
Inoculate the Korean pine nursery stock height of seedling of bolete of the present invention (Suillus sp.) HDSF2, growth rapidly, absorption of nutrient ingredients rate height such as big, the nitrogen phosphorus potassium of biomass, cane is sturdy, dark green leaf color is glossy, the survival rate height.Bolete HDSF2 of the present invention can be used for the Korean pine artificial breeding.
Bolete of the present invention (Suillus sp.) HDSF2 is the most approaching with thick ring suillusbovinus through the autoploidy comparison, but the form difference obviously is different from thick ring suillusbovinus, through being accredited as the new ectotrophic mycorrhiza fungi of a strain.
Bolete of the present invention (Suillus sp.) HDSF2 belongs to Boletus (Boletus), be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, the preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, preservation date is on September 17th, 2010, and preserving number is CGMCC No.4175.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises any combination between each embodiment.
Embodiment one: the ectotrophic mycorrhiza fungi of present embodiment and the efficient symbiosis of Korean pine is bolete (Suillus sp.) HDSF2, is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC No.4175.
To large-scale, bacteria cover diameter is 6～12cm, be flat hemispherical, the back center projections, central recessed, smooth, sticking, color is a russet sometimes in the fruit body of present embodiment bolete (Suillus sp.) HDSF2.Bolete HDSF2 bacterial context is faint yellow; The tube initial colour is light, and the thin out lark in back is hindered thin out aubergine or band brown, straight giving birth to closely prolonging life; The mouth of pipe is less, dihedral, and part is compound, 1～2 every millimeter; Handle length is 6～12cm, thick 1～2.6cm, and nearly cylindricality is omited etc. thick up and down or base portion is thin slightly, no gland point, there is reticulate pattern on the top.Bolete (Suillus sp.) HDSF2 spore is to print yellowish-brown to Chestnut, and the oval or near spindle of spore is level and smooth, is with olive-yellow, and length and width are (8.7～10.4 μ m) * (3.5～4.2 μ m); How bar-shaped pipe flange is colourless to filbert, scattered to clustering, with pipe side utricule, and length and width are (26～83 μ m) * (5.2～6 μ m).
Present embodiment bolete (Suillus sp.) HDSF2 can cultivate with potato culture or improvement MMN medium, and optimum growth temp is 23 ℃, and growth is best under the pH5.5 environment.
Present embodiment bolete (Suillus sp.) HDSF2 is collected in the medium-and-large-sized fungus sporophore of the Yichun Xiaoxinanlin Mountains, Heilongjiang Province Feng Lin national natural reserves Korean pine broad leaved forest.
Embodiment two: will grow on the sterilization matrix of the laboratory Korean pine nursery stock in 54 weeks of present embodiment is divided into 2 groups at random, first group of Korean pine nursery stock inoculation embodiment one bolete HDSF2, and every Korean pine nursery stock bolete HDSF2 inoculum concentration is 120mL.Second group of Korean pine nursery stock be not as blank group (inoculating bolete HDSF2).All the other are identical with conventional Korean pine nursery stock artificial planting type of rearing.
Present embodiment adopts punching to inject inoculation bolete HDSF2, and (distance＜5cm) 4 places are annotated in the hole for hole and nursery stock, and 30mL bolete HDSF2 culture fluid is annotated in hole, every hole around every Korean pine nursery stock.Bolete HDSF2 mycelium concentration is in the culture fluid: mycelia dry weight 12g/L.
First group of Korean pine nursery stock bolete (Suillus sp.) HDSF2 infection rate is that 86.42%, the second group of Korean pine nursery stock is not found mycorhiza after 20 weeks of bolete HDSF2 inoculation.
Korean pine nursery stock inoculation growth 20 week back is measured the every index of Korean pine nursery stock, and test result is as shown in table 1.
Table 1 Korean pine seedling growth index
| First group | Second group | |
| Mycorhiza infection rate (%) | 86.42 | 0 |
| Average height of seedling (cm) | 11.23 | 7.50 |
| Average biomass (g/ strain) | 9.25 | 6.47 |
| Nursery stock leading thread (mm) | 8.2 | 6.6 |
| Plant nitrogen content (%) | 1.78 | 1.33 |
| Plant phosphorus content (%) | 0.147 | 0.091 |
| Plant potassium content (%) | 0.66 | 0.39 |
| The leaf look | Bottle green | Yellow green |
| Survival rate (%) | 100 | 86 |
First group of Korean pine seedling growth is vigorous, obviously is better than second group of Korean pine nursery stock, and nitrogen phosphorus potassium absorption ratio significantly promotes.
The ectotrophic mycorrhiza of getting the first group Korean pine nursery stock of inoculation after 20 weeks carries out that the ectotrophic mycorrhiza fungi separates, purifying, then the ectotrophic mycorrhiza fungi that obtains is repeated above-mentioned symbiosis contrast experiment 2 times, the discovery experiment effect is identical, and bolete (Suillus sp.) HDSF2 proterties, function-stable are described.
Embodiment three: present embodiment is together carried out Korean pine symbiosis experiment with thick ring suillusbovinus (Suillus grevillei), the inferior suillus lutens (Suillus subluteus) of record in " Daqunshan Mountains ectotrophic mycorrhiza fungal culture characteristic and Chinese pine mycorhiza study on the synthesis " (author Lu Lijun, " Agricultural University of the Inner Mongol " 2005) with embodiment one bolete HDSF2.
Vaccination ways is identical with embodiment two, and all the other are identical with conventional Korean pine nursery stock artificial planting type of rearing.
Thick ring suillusbovinus (Suillus grevillei) infection rate is 68.56% after 20 weeks of inoculation, inferior suillus lutens (Suillussubluteus) is not set up symbiotic relation with Korean pine, and bolete (Suillus sp.) HDSF2 infection rate is 86.42%.
Korean pine nursery stock inoculation growth 20 week back is measured every index, and test result is as shown in table 2.
Table 2 is with fungal bacterial strain inoculation Korean pine nursery stock result not of the same race in the Pseudomonas
Present embodiment symbiosis experiment finds that embodiment one bolete HDSF2 is the efficient symbiosis ectotrophic mycorrhiza of a Korean pine fungi, inoculate this ectotrophic mycorrhiza fungi and can improve the Korean pine height of seedling, growth rate and biomass, and increase absorption of nutrient ingredients rate such as nitrogen phosphorus potassium, make the Korean pine cane of artificial culture sturdy, dark green leaf color is glossy, the survival rate height; For the technology popularization of Korean pine artificial breeding is laid a good foundation.
Claims (1)
1. the ectotrophic mycorrhiza fungi of a strain and the efficient symbiosis of Korean pine, it is bolete (Suillus sp.) HDSF2, is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is CGMCC No.4175.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696466A (en) * | 2012-06-25 | 2012-10-03 | 上海市园林科学研究所 | Method for quick mycorhiza formation of azalea aseptic seedlings |
| CN106171515A (en) * | 2016-07-08 | 2016-12-07 | 云南大学 | A kind of Applying Ectomycorrhizal Fungi and the symbiosis method for building up of pine genus plant |
| CN112997800A (en) * | 2020-12-04 | 2021-06-22 | 郑永彬 | Boletaceae fungus and application thereof |
-
2010
- 2010-11-19 CN CN201010551671A patent/CN102007869B/en not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
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| 《微生物学通报》 19830331 武占元，等 红松菌根的研究 1 , 第3期 * |
| 《林业科技》 20090531 张晓兵,等 外生菌根菌对红松苗木生长的影响 30-32 1 第34卷, 第3期 * |
| 《黑龙江省环境报》 19990331 齐恒玉，等 红松苗菌根菌的初步研究 1 第23卷, 第1期 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696466A (en) * | 2012-06-25 | 2012-10-03 | 上海市园林科学研究所 | Method for quick mycorhiza formation of azalea aseptic seedlings |
| CN102696466B (en) * | 2012-06-25 | 2013-08-21 | 上海市园林科学研究所 | Method for quick mycorhiza formation of azalea aseptic seedlings |
| CN106171515A (en) * | 2016-07-08 | 2016-12-07 | 云南大学 | A kind of Applying Ectomycorrhizal Fungi and the symbiosis method for building up of pine genus plant |
| CN106171515B (en) * | 2016-07-08 | 2019-03-19 | 云南大学 | A kind of symbiosis method for building up of Applying Ectomycorrhizal Fungi and pine genus plant |
| CN112997800A (en) * | 2020-12-04 | 2021-06-22 | 郑永彬 | Boletaceae fungus and application thereof |
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