CN101985507A - Method for preparing super absorbent resin containing composite plant growth regulator and dehydrated Murashige and Skoog's (MS) culture medium - Google Patents
Method for preparing super absorbent resin containing composite plant growth regulator and dehydrated Murashige and Skoog's (MS) culture medium Download PDFInfo
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- CN101985507A CN101985507A CN 201010501272 CN201010501272A CN101985507A CN 101985507 A CN101985507 A CN 101985507A CN 201010501272 CN201010501272 CN 201010501272 CN 201010501272 A CN201010501272 A CN 201010501272A CN 101985507 A CN101985507 A CN 101985507A
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Abstract
The invention discloses a method for preparing a super absorbent resin containing a composite plant growth regulator and a dehydrated Murashige and Skoog's (MS) culture medium. In the method, sodium o-nitrophenolate/ sodium p-nitrophenolate, sodium fulvate and the dehydrated MS culture medium are added in the synthesis process of the super absorbent resin, and the super absorbent resin is used in place of agar as a culture medium coagulant for allowing the culture medium to reach a proper coagulability, supporting cultured tissues, allowing the nutrients to disperse in the super absorbent resin easily and improving the absorption rate of nutrients. Meanwhile, because the super absorbent resin has high water absorbing and retaining capacities and water absorbing and releasing reversibility, plant tissues can use water effectively, irrigation times is reduced, and water and labor are saved. The synthesis process is simple, the product contains the composite plant growth regulator, and thus, the resin has a promising application prospect and a high promotion value.
Description
Technical field
The present invention relates to a kind of preparation method of novel super absorbent resin, especially relate to a kind of preparation method who contains composite plant growth regulator and dehydration MS substratum High hydrophilous resin, the High hydrophilous resin of the present invention's preparation mainly is applicable to the plant tissue culture aspect.
Background technology
High hydrophilous resin (Super Absorbent Polymer is called for short SAP) is a kind of the have excellent water-absorbent and new functional macromolecule material of water-retentivity, it can absorb the water of own wt hundred times or thousands of times and swelling is gel, water can not separated out yet under the pressure of certain limit, can recover its water absorbing properties again after the resin gel thing drying after the suction, thereby important use is all arranged in a lot of fields.As being applied to baby's paper nappy, sanitary napkin, medicament slow release material etc. aspect the medical and health, in industry and construction industry the waterproof etc. that blocks water as oil-water separating agent, siccative, deodorizing composition, cable and tunnel construction, on agricultural as soil improvement, desert afforestation, soil-less culture medium, drought-resistant water-preserving, agriculture chemical slowly-releasing, food and fruit freshness preserving material etc.
The MS substratum is that Murashige and Skoog cultivated design in 1962 for tobacco cell, be characterized in that inorganic salt and ionic concn are higher, has more stable ionic equilibrium system, its nitrate, sylvite and amounts of ammonium salt height, the kind and the ratio of its nutrient are reasonable, can satisfy the nutrition and the physiological requirements of vegetable cell, thereby the scope of application are wider, most plant tissue culture and quick breeding all use it as substratum, are to use the most general substratum at present.But because the MS substratum in actual use, moisture evaporation speed is fast, brings a lot of inconvenience to tissue culture procedures, has limited the application of MS substratum.
O/p-nitrophenol sodium and SODLUM FULVATE all are the broad spectrum type plant-growth regulator, all have the cellular plasm of promotion and flow, improve cell viability, accelerate the speed of growth, breaking dormancy promotes to grow, and prevents fruit drop, improve product quality, improve output, strengthen the plant immunization ability, reduce pathogen infection, improve disease-resistant, pest-resistant, the drought resisting of crop, the advantage of waterlogging-resistant, cold-resistant, anti-anti-adversity ability such as saline and alkaline, resistant to lodging.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of preparation method who contains composite plant growth regulator and dehydration MS substratum High hydrophilous resin, this method is to be raw material with vinylformic acid, acrylamide, sodium hydroxide, dehydration MS substratum, o/p-nitrophenol sodium, SODLUM FULVATE etc., through reasonable proportioning, make by certain technological process and synthesis technique.
Technical scheme of the present invention is as follows: under the ice-water bath cooling and stirring, remove the vinylformic acid of stopper with the sodium hydroxide solution neutralization, obtain vinylformic acid and sodium salt solution thereof, in reactor, add dehydration MS substratum then, acrylamide soln, SODLUM FULVATE and o/p-nitrophenol sodium solution, linking agent N, N '-methylene-bisacrylamide, initiator potassium persulfate, at room temperature stir 1.0-1.5h, it is mixed, wherein, vinylformic acid and acrylamide, dehydration MS substratum, sodium hydroxide, o/p-nitrophenol sodium, SODLUM FULVATE, N, N '-methylene-bisacrylamide, the mass ratio of Potassium Persulphate is 1: 0-0.5: 0.2-3.2: 0.15-0.62: 0.0002-0.0599: 0.0002-0.0599: 0.0002-0.0045: 0.0016-0.0089, feed nitrogen afterwards and slowly heating in water-bath, make it react 1h down at 65-85 ℃, continue to heat up, under the condition of boiling water bath heating, be incubated 1.5h again, stop logical nitrogen, be cooled to room temperature, product is taken out, oven dry, pulverize, sieve, promptly get the High hydrophilous resin that contains composite plant growth regulator and dehydration MS substratum, the suction multiple of product is 290-590 times.
The present invention has the following advantages:
(1) after the High hydrophilous resin suction certain gel-strength is arranged, therefore replace the peptizer of agar with it as substratum, can make substratum reach suitable solidifiability, cultured tissue there is suitable supporting role, can make again and nutritive substance diffusion easily in absorbent resin improve nutrient absorbing efficient.
(2) High hydrophilous resin water-absorbing-retaining ability is strong, also is difficult for dehydration during pressurization, has the reversibility that absorbs water and release water, helps plant tissue and effectively utilizes moisture, reduces the number of times that waters, water saving, saving of labor.
(3) the present invention adopts and add dehydration MS substratum in High hydrophilous resin synthetic process, need only in proportion product be joined the plant tissue culture media that can be made into water-maintaining in distilled water or the tap water during use.
(4) because the growth of different plants requires different acid or alkali environments, so the pH value of plant tissue culture media often becomes because of the plant of cultivating is different.And synthetic High hydrophilous resin of the present invention can use under the acid or alkali environment comparatively widely, and the coagulation result of substratum is unaffected.
(5) the prepared product of the present invention also contain can coordinate plant growth o/p-nitrophenol sodium and SODLUM FULVATE.
(6) synthesis technique of the present invention is simple, and the no three wastes produce, and help suitability for industrialized production.
Description of drawings
Fig. 1 is preparation technology's schema of product
Fig. 2 is the infrared spectrogram of embodiment 7 products
Embodiment
Embodiment 1: with 19ml deionized water dissolving 6.0g sodium hydroxide, under the ice-water bath cooling and stirring, in and 27g (25.6ml) vinylformic acid; With 25ml deionized water dissolving 0.1g SODLUM FULVATE; 0.05g sodium onitrophenol and 0.05g Sodium p-nitrophenoxide; the vinylformic acid and the sodium salt solution thereof of becoming reconciled in adding afterwards; 10g dehydration MS substratum; the 100mg Potassium Persulphate; 30mgN; N '-methylene-bisacrylamide; stir 1.0h under the room temperature; slowly heating under nitrogen protection then; make it react 1h down, continue to heat up, in boiling water bath, be incubated 1.5h again at 60-80 ℃; stop logical nitrogen; be cooled to room temperature, product is taken out, oven dry; pulverize; sieve, promptly get the High hydrophilous resin that contains composite plant growth regulator and dehydration MS substratum, the suction multiple of present embodiment products obtained therefrom is 570 times.
Embodiment 2: experiment condition is with embodiment 1, and different is that the suction multiple of present embodiment products obtained therefrom is 590 times with 25ml deionized water dissolving 0.01g SODLUM FULVATE, 0.01g sodium onitrophenol and 0.01g Sodium p-nitrophenoxide.
Embodiment 3: experiment condition is with embodiment 1, and different is that the suction multiple of present embodiment products obtained therefrom is 450 times with 25ml deionized water dissolving 0.1g SODLUM FULVATE, 0.1g sodium onitrophenol and 0.1g Sodium p-nitrophenoxide.
Embodiment 4: experiment condition is with embodiment 1, and different is that the suction multiple of present embodiment products obtained therefrom is 580 times with 25ml deionized water dissolving 0.1g SODLUM FULVATE, 0.01g sodium onitrophenol and 0.05g Sodium p-nitrophenoxide.
Embodiment 5: experiment condition is with embodiment 1, different is with 25ml deionized water dissolving 0.5g SODLUM FULVATE, 0.02g sodium onitrophenol and 0.04g Sodium p-nitrophenoxide, 35mgN, N '-methylene-bisacrylamide, the suction multiple of present embodiment products obtained therefrom is 540 times.
Embodiment 6: experiment condition is with embodiment 1, different is with 25ml deionized water dissolving 0.3g SODLUM FULVATE, 0.3g sodium onitrophenol and 0.3g Sodium p-nitrophenoxide, 40mgN, N '-methylene-bisacrylamide, the suction multiple of present embodiment products obtained therefrom is 380 times.
Embodiment 7: with 24ml deionized water dissolving 6.0g sodium hydroxide, under the ice-water bath cooling and stirring, in and 15.73g (15ml) vinylformic acid; With 25ml deionized water dissolving 0.1g SODLUM FULVATE; 0.1g sodium onitrophenol and 0.1g Sodium p-nitrophenoxide; the vinylformic acid and the sodium salt solution thereof of becoming reconciled in adding afterwards; 7.87g acrylamide; 10g dehydration MS substratum; the 100mg Potassium Persulphate; 30mgN; N '-methylene-bisacrylamide; stir 1.0h under the room temperature; slowly heating under nitrogen protection then; make it react 1h down at 60-80 ℃; continue to heat up; in boiling water bath, be incubated 1.5h again; stop logical nitrogen; be cooled to room temperature, product is taken out, oven dry; pulverize; sieve, promptly get the High hydrophilous resin that contains composite plant growth regulator and dehydration MS substratum, the suction multiple of present embodiment products obtained therefrom is 430 times.
Embodiment 8: experiment condition is with embodiment 7, and different is that the suction multiple of present embodiment products obtained therefrom is 440 times with 25ml deionized water dissolving 0.1g SODLUM FULVATE, 0.01g sodium onitrophenol and 0.05g Sodium p-nitrophenoxide.
Embodiment 9: experiment condition is with embodiment 7, and different is that the suction multiple of present embodiment products obtained therefrom is 470 times with 25ml deionized water dissolving 0.1g SODLUM FULVATE, 0.05g sodium onitrophenol and 0.05g Sodium p-nitrophenoxide.
Embodiment 10: experiment condition is with embodiment 7, and different is that the suction multiple of present embodiment products obtained therefrom is 410 times with 25ml deionized water dissolving 0.01g SODLUM FULVATE, 0.01g sodium onitrophenol and 0.01g Sodium p-nitrophenoxide.
Embodiment 11: experiment condition is with embodiment 7, different is with 25ml deionized water dissolving 0.5g SODLUM FULVATE, 0.02g sodium onitrophenol and 0.04g Sodium p-nitrophenoxide, 35mgN, N '-methylene-bisacrylamide, the suction multiple of present embodiment products obtained therefrom is 340 times.
Embodiment 12: experiment condition is with embodiment 7, different is with 25ml deionized water dissolving 0.3g SODLUM FULVATE, 0.3g sodium onitrophenol and 0.3g Sodium p-nitrophenoxide, 40mgN, N '-methylene-bisacrylamide, the suction multiple of present embodiment products obtained therefrom is 290 times.
The infrared spectra of embodiment 7 products is by NEXUS
TM670FT-IR E.S.P type determination of infrared spectroscopy.Measure through German Elementar Vario ELIII type ultimate analysis instrument, the constituent content of embodiment 7 products is: C%=36.16, H%=6.330, N%=4.198.
Claims (1)
1. one kind contains composite plant growth regulator and the preparation method of the MS substratum High hydrophilous resin that dewaters, it is characterized in that: under the ice-water bath cooling and stirring, remove the vinylformic acid of stopper with the sodium hydroxide solution neutralization, obtain vinylformic acid and sodium salt solution thereof, in reactor, add dehydration MS substratum then, acrylamide soln, SODLUM FULVATE and o/p-nitrophenol sodium solution, linking agent N, N '-methylene-bisacrylamide, initiator potassium persulfate, at room temperature stir 1.0-1.5h, it is mixed, wherein, vinylformic acid and acrylamide, dehydration MS substratum, sodium hydroxide, o/p-nitrophenol sodium, SODLUM FULVATE, N, N '-methylene-bisacrylamide, the mass ratio of Potassium Persulphate is 1: 0-0.5: 0.2-3.2: 0.15-0.62: 0.0002-0.0599: 0.0002-0.0599: 0.0002-0.0045: 0.0016-0.0089, feed nitrogen afterwards and slowly heating in water-bath, make it react 1h down at 65-85 ℃, continue to heat up, under the condition of boiling water bath heating, be incubated 1.5h again, stop logical nitrogen, be cooled to room temperature, product is taken out, oven dry, pulverize, sieve, promptly get the High hydrophilous resin that contains composite plant growth regulator and dehydration MS substratum, the suction multiple of product is 290-590 times.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010105012721A CN101985507B (en) | 2010-10-09 | 2010-10-09 | Method for preparing super absorbent resin containing composite plant growth regulator and dehydrated Murashige and Skoog's (MS) culture medium |
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| CN2010105012721A CN101985507B (en) | 2010-10-09 | 2010-10-09 | Method for preparing super absorbent resin containing composite plant growth regulator and dehydrated Murashige and Skoog's (MS) culture medium |
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| CN101985507B CN101985507B (en) | 2011-09-21 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283605A (en) * | 2013-06-17 | 2013-09-11 | 何寒 | Preparation method of tissue culture medium without agar |
| CN103329800A (en) * | 2013-06-17 | 2013-10-02 | 何寒 | Tissue culture medium without agar during plant open tissue culturing and preparation method of tissue culture medium |
| CN113980164A (en) * | 2021-10-29 | 2022-01-28 | 潍坊科技学院 | Preparation method of super absorbent resin containing plant growth promoter |
Citations (4)
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| US4997581A (en) * | 1988-11-14 | 1991-03-05 | Nalco Chemical Company | Additive and method for temporarily reducing permeability of subterranean formations |
| CN1319618A (en) * | 2001-02-27 | 2001-10-31 | 北京市朝阳区科龙达新技术应用研究所 | Holoside water retaining agent and preparation method thereof |
| CN101638456A (en) * | 2009-09-14 | 2010-02-03 | 内蒙古大学 | Method for preparing super absorbent resin with cross-linked structure by using potato starch as raw material |
| CN101658459A (en) * | 2008-08-29 | 2010-03-03 | 上海市长宁区少年科技指导站 | Walk aid blind guiding stick |
-
2010
- 2010-10-09 CN CN2010105012721A patent/CN101985507B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4997581A (en) * | 1988-11-14 | 1991-03-05 | Nalco Chemical Company | Additive and method for temporarily reducing permeability of subterranean formations |
| CN1319618A (en) * | 2001-02-27 | 2001-10-31 | 北京市朝阳区科龙达新技术应用研究所 | Holoside water retaining agent and preparation method thereof |
| CN101658459A (en) * | 2008-08-29 | 2010-03-03 | 上海市长宁区少年科技指导站 | Walk aid blind guiding stick |
| CN101638456A (en) * | 2009-09-14 | 2010-02-03 | 内蒙古大学 | Method for preparing super absorbent resin with cross-linked structure by using potato starch as raw material |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283605A (en) * | 2013-06-17 | 2013-09-11 | 何寒 | Preparation method of tissue culture medium without agar |
| CN103329800A (en) * | 2013-06-17 | 2013-10-02 | 何寒 | Tissue culture medium without agar during plant open tissue culturing and preparation method of tissue culture medium |
| CN113980164A (en) * | 2021-10-29 | 2022-01-28 | 潍坊科技学院 | Preparation method of super absorbent resin containing plant growth promoter |
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| CN101985507B (en) | 2011-09-21 |
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Effective date of registration: 20161207 Address after: 530022 the Guangxi Zhuang Autonomous Region Nanning Qingxiu District National Road No. 93 Xinxing Hotel, 19 storey office building A2, room A3 Patentee after: Guangxi Shen letter Technology Co. Ltd. Address before: 010021 Hohhot West Road, the Inner Mongolia Autonomous Region, No. 235 Patentee before: Inner Mongolia University |