CN101980214A - Method for constructing porosity-controlled bionic scaffold - Google Patents
Method for constructing porosity-controlled bionic scaffold Download PDFInfo
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- CN101980214A CN101980214A CN 201010186215 CN201010186215A CN101980214A CN 101980214 A CN101980214 A CN 101980214A CN 201010186215 CN201010186215 CN 201010186215 CN 201010186215 A CN201010186215 A CN 201010186215A CN 101980214 A CN101980214 A CN 101980214A
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Abstract
The invention relates to a method for constructing a porosity-controlled bionic scaffold, which comprises the following steps of: scanning the entire natural bone by using Micro-CT technology, extracting spongy bone data and reconstructing a porous structure model of a spongy bone; measuring the porosity of the spongy bone model by using Mimics; then constructing a unit body with a proper porous structure according to the porosity; processing the unit body by using an image to obtain a three-dimensional porous structure model; and finally, performing Boolean intersection operation on the three-dimensional porous structure model and a damaged bone model so as to construct a porous structure model of the bionic scaffold, which is matched with the damaged part. In the method, the porosity corresponding to the natural bone can be obtained in the process of reconstructing and measuring, the characteristics of the natural bone can be better simulated in construction, and cell adhesion, crawling and bone replacement are more convenient. The bone scaffold constructed by the method has the same outline as real bone, which better contributes to implantation of the scaffold. A parameterized construction method can adjust different porosity characteristics of different natural bones and makes scaffold construction convenient. A construction method for obtaining the unit body by processing a unit body image solves the problem of porosity communication in a microstructure.
Description
Technical field
The present invention relates to a kind of construction method of artificial bone of orthopaedic medical treatment reparation usefulness, particularly a kind of structure of biomimetic scaffolds of the controlled porosity based on natural bone.
Background technology
Along with developing rapidly and the raising of people's living standard, health care, rehabilitation outcome of material science and medical technology, the aging of population in addition, people are urgent day by day to the requirement of aspects such as damaged reparation of tissue, organ and bone and displacement.Repair because the diverse bone types that diseases such as wound, infection, tumour cause is damaged, especially big section of limbs long bone backbone damaged, be the difficult problem that the orthopedist faces always.It is conventional methods of treatment that bone is transplanted, but limited from body bone source, allogenic bone transplantation then exists immunological rejection and risk of disease transmission.In recent years, the rise of bone tissue engineer is that the damaged repairing and treating of bone has been opened up new research field, and Gegenbaur's cell and support carrier are compounded in external co-incubation, is the initial sum key that bone tissue engineer makes up, and also is the focus of studying both at home and abroad at present.At this situation, a kind of new solution---Bionic Design and manufacturing have been proposed.
The structure of the artificial bone of biologically active is one of bionic important research content.It is a kind of by biodegradable natural or synthetic three-dimensional cell support in the inside of human body implantation, and injects a kind of molecule that is called growth factor.Cell is according to the three-dimensional rack of prefabricated form propagation, differentiation, and meanwhile support is progressively degraded, last in human body, generate one with the function of organizing originally and the new bone of form basically identical.
Biomimetic scaffolds is one of three elements of bone tissue engineer, and support not only provides support structure for specific cell, and can also guide tissue regeneration, the control institutional framework.In order to satisfy these requirements, biomimetic scaffolds the microcosmic porous structure make up and just seem particularly important.
At present, along with the maturation of biological CAD modeling technique and rapid shaping technique, the three-dimensional reconstruction of bone tissue porous structure becomes possibility.But in process of reconstruction, by the structure of the support micropore structure of artificial design and natural reality bone big difference is arranged still very much, the porosity of microstructure and connectedness can not be controlled, are difficult to guarantee fully the functional of its structure; And the conventional stent method for building up is operation parameter structure thinking scarcely, causes the porosity of the biomimetic scaffolds of foundation in time not make adjustment at the natural bone characteristic of sufferer.
Summary of the invention
The objective of the invention is to deficiency, a kind of construction method of the biomimetic scaffolds based on parameterized controlled porosity is provided, make up more convenient victory at prior art; The micromechanism of the internal capillary support model of gained and porosity and natural bone are more approaching, and the cultivation and the skeletonization that more help cell substitute; Internal microcellular structure connection rate reaches 100%; Its appearance profile meets broken bone parts, helps the implantation of artificial bone.
In order to achieve the above object, the present invention's design is: at first use Micro-CT that natural bone is carried out entire scan, with the microcosmic three-dimensional micropore structural information of acquisition natural reality bone and the cross-section image of three-dimensional space position density information, utilize the then different principle of threshold value of its image of different tissue density's differences then, each faultage image is carried out threshold process, distinguish tissue, obtain the image of its binaryzation.Extract the part of cancellous bone in the above-mentioned 2-D data, behind the three-dimensional model parameter value that setting generates, just can obtain the cancellous bone porous structure model that makes up by numerical data.Next uses Mimics to calculate the porosity and the perforation rate of the cancellous bone that makes up.In UGNX, set up the good parameterized units phantom type of pore texture by this porosity.The gained unit model is carried out mirror image processing by the required size of broken bone, obtain a porous structure model that is slightly larger than broken bone size.The solid model of this model and broken bone is carried out boolean's cap, can obtain the biomimetic scaffolds model of required microcellular structure.
According to the foregoing invention design, the present invention adopts following technical proposals:
A kind of structure of the biomimetic scaffolds porous structure based on parameterized controlled porosity is characterized in that operation steps is as follows:
(1) use Micro-CT that selected natural bone is carried out entire scan:
Use Micro-CT that selected natural bone integral body is carried out entire scan, with the microcosmic three-dimensional micropore structural information of acquisition natural reality bone and the cross-section image of three-dimensional space position density information;
(2) utilize threshold method with the image of Micro-CT data processing for binaryzation:
The faultage image that above-mentioned each Micro-CT scanning is obtained uses threshold method to carry out binary conversion treatment, and two kinds of image gray of black and white are only arranged;
(3) the cancellous bone porous structure model of reconstruction natural bone:
In above-mentioned gray level image, choose the wherein image-region of cancellous bone, behind the three-dimensional model parameter value that setting generates, just can obtain the cancellous bone porous structure model that makes up by numerical data;
(4) porosity and the perforation rate of use Mimics computed in software model:
With calculating in the importing of the cancellous bone porous structure model behind the three-dimensional reconstruction Mimics software, obtain correlation parameter;
(5) set up unit model under the UG NX:
Foundation is set up the good parameterized units phantom type of pore texture by this porosity;
(6) cell cube is carried out mirror image processing:
Unit model is carried out whole convergent-divergent, obtain the unit model that microcellular structure is complete, be of a size of 2x2x2mm.This unit model is carried out mirror image processing, can obtain required porous structure model;
(7) obtain the support model:
Set up the solid model of true bone, will obtain the three-dimensional micropore structural model of size, carry out boolean's cap, promptly obtain required porous structure support model with true bone model by mirror image processing greater than true bone model.
The present invention compared with prior art, have following conspicuous outstanding substantive distinguishing features and remarkable advantage: the cancellous bone porous structure model that obtains behind Micro-CT scan-data, binary image and the three-dimensional reconstruction is carried out correlation data calculation with Mimics software, obtain its porosity, carry out the parameterized units volume modeling, porous structure model by the cell cube mirror image is made up again, because of its micropore structure and porosity natural imitation bone well, have the superperformance similar, have bionical meaning to natural bone.The parameterized units volume modeling has been accelerated the modeling speed of microcellular structure biomimetic scaffolds greatly.At the different situation of different natural bone porositys the time, only need to change a unit model parameter and just can set up the different microcellular structure support model of porosity like this, alleviated modeling difficulty, promoted the speed of modeling.Wherein the method that cell cube is carried out mirror image processing has solved the problem of the porosity communication in the microstructure, and by the porous structure model that mirror image processing obtains, can require to obtain arbitrarily the model of various sizes according to need.Model behind the mirror image is carried out boolean with true bone model ships calculation, the appearance profile that has guaranteed porous structure support model and true bone photo with, it is better identical to help support and damaged portion.The inner porous structure of the biomimetic scaffolds that makes by the method is close with true bone structure, its good internal microstructure more help cell adhesion, creep and the skeletonization alternative Process; Peripheral profile more helps the implantation of support with truly bone photo together can be better identical with breakage.
Description of drawings
Fig. 1 is the flow chart of structure bone support method of the present invention.
Fig. 2 is the cancellous bone microcosmic porous structure figure that the present invention obtains after by Micro-CT scan-data, binaryzation and three-dimensional reconstruction.
Fig. 3 is that the present invention calculates the lab diagram that cancellous bone obtains porosity by Mimics.
Fig. 4 is that the present invention is according to calculating the suitable unit model figure of structure that the gained porosity makes up.
The illustraton of model that the mirror image processing that Fig. 5 is the present invention by the model unit body obtains.
Fig. 6 is that the model of the present invention after by mirror image carries out boolean with true bone model and ship the support illustraton of model that obtains.
Embodiment
A preferred embodiment of the present invention, be described with reference to the accompanying drawings as follows: the construction method concrete operations step following (referring to Fig. 1) of the biomimetic scaffolds of this controlled porosity: at first, use Micro-CT that natural bone is scanned, with the microcosmic three-dimensional micropore structural information of acquisition cancellous bone and the faultage image of three-dimensional space position density information.Above-mentioned each faultage image is carried out threshold process, obtain the image of binaryzation.Choose the wherein data of cancellous bone, behind the three-dimensional model parameter value that setting generates, just can obtain the cancellous bone porous structure model (referring to Fig. 2) that makes up by numerical data.Subsequently, place Mimics to carry out the calculating of correlation parameters such as porosity on cancellous bone porous structure model, referring to Fig. 3.Then, according to calculating the gained porosity, connectivity requirement etc. make up the suitable unit model of structure, are of a size of 2 * 2 * 2mm, referring to Fig. 4.Then, by mirror image processing repeatedly, the model unit body is built into the porous structure model with good communicating structure, its size is greater than the model of true bone, referring to Fig. 5.At last, the solid model of the above-mentioned microcellular structure model that obtains and true bone is carried out boolean's cap, promptly obtain the support model of needed microcellular structure, referring to Fig. 6.
Claims (1)
1. the construction method of the biomimetic scaffolds of a controlled porosity is characterized in that operation steps is as follows:
(1) use Micro-CT that selected natural bone is carried out entire scan:
Use Micro-CT that selected natural bone integral body is carried out entire scan, with the microcosmic three-dimensional micropore structural information of acquisition natural reality bone and the cross-section image of three-dimensional space position density information;
(2) utilize threshold method with the image of Micro-CT data processing for binaryzation:
The faultage image that above-mentioned each Micro-CT scanning is obtained uses threshold method to carry out binary conversion treatment, and two kinds of image gray of black and white are only arranged;
(3) the cancellous bone porous structure model of reconstruction natural bone:
In above-mentioned gray level image, choose the wherein image-region of cancellous bone, behind the three-dimensional model parameter value that setting generates, just can obtain the cancellous bone porous structure model that makes up by numerical data;
(4) porosity and the perforation rate of use Mimics computed in software model:
With calculating in the importing of the cancellous bone porous structure model behind the three-dimensional reconstruction Mimics software, obtain correlation parameter;
(5) set up unit model under the UG NX:
Foundation is set up the good parameterized units phantom type of pore texture by this porosity;
(6) cell cube is carried out mirror image processing:
Unit model is carried out whole convergent-divergent, obtain the unit model that microcellular structure is complete, be of a size of 2x2x2mm; This unit model is carried out mirror image processing, can obtain required porous structure model;
(7) obtain the support model:
Set up the solid model of true bone, will obtain the three-dimensional micropore structural model of size, carry out boolean's cap, promptly obtain required porous structure support model with true bone model by mirror image processing greater than true bone model.
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102426711A (en) * | 2011-09-08 | 2012-04-25 | 上海大学 | Three-dimensional porous bone scaffold discrete model construction method capable of controlling discrete interval |
| CN102499794A (en) * | 2011-11-04 | 2012-06-20 | 西北工业大学 | Preparation method of artificial bone support with controllable porosity |
| CN102716512A (en) * | 2012-06-29 | 2012-10-10 | 上海大学 | Method for preparing medicine graded sustained-release bone repair body |
| CN102973334A (en) * | 2012-12-24 | 2013-03-20 | 天津大学 | Bionic design method of skull tissue engineering scaffold |
| CN104573218A (en) * | 2015-01-04 | 2015-04-29 | 秦晓亮 | Optimizing and fast modeling method for three-dimensional printing hole |
| CN105930617A (en) * | 2016-05-17 | 2016-09-07 | 南方医科大学 | Method for designing and forming stiffness-controllable bone tumor defect repair implant |
| CN105976425A (en) * | 2016-04-13 | 2016-09-28 | 深圳市艾科赛龙科技有限公司 | Method and device for structural design |
| CN105997306A (en) * | 2016-04-25 | 2016-10-12 | 北京工业大学 | Design method for filling porous grid structure in bone implantation body |
| CN106923936A (en) * | 2017-03-31 | 2017-07-07 | 中国人民解放军第四军医大学 | The design preparation method of the personalized customization 3D printing porous titanium alloy segmental prosthese rebuild for large segmental bone defect |
| CN107997855A (en) * | 2017-11-30 | 2018-05-08 | 深圳先进技术研究院 | 3D porous supports method for establishing model, device and preparation system |
| WO2020114253A1 (en) * | 2018-12-04 | 2020-06-11 | 北京市春立正达医疗器械股份有限公司 | Bone trabecula structure and prosthesis using same and manufacturing method therefor |
| CN111899346A (en) * | 2020-07-16 | 2020-11-06 | 天津理工大学 | Mathematical modeling method of controllable porous bone tissue engineering scaffold |
| CN113768665A (en) * | 2020-06-10 | 2021-12-10 | 上海交通大学医学院附属第九人民医院 | Bone defect repair support, construction method, preparation method, computer-readable storage medium and equipment |
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| CN1609899A (en) * | 2004-10-27 | 2005-04-27 | 上海大学 | Bionic stent generating method based on CT picture |
| CN1669538A (en) * | 2005-04-08 | 2005-09-21 | 卢建熙 | Artificial bone with biology imitating structure, preparation method and application thereof |
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- 2010-05-26 CN CN 201010186215 patent/CN101980214A/en active Pending
Patent Citations (2)
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| CN1609899A (en) * | 2004-10-27 | 2005-04-27 | 上海大学 | Bionic stent generating method based on CT picture |
| CN1669538A (en) * | 2005-04-08 | 2005-09-21 | 卢建熙 | Artificial bone with biology imitating structure, preparation method and application thereof |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102426711A (en) * | 2011-09-08 | 2012-04-25 | 上海大学 | Three-dimensional porous bone scaffold discrete model construction method capable of controlling discrete interval |
| CN102499794A (en) * | 2011-11-04 | 2012-06-20 | 西北工业大学 | Preparation method of artificial bone support with controllable porosity |
| CN102716512A (en) * | 2012-06-29 | 2012-10-10 | 上海大学 | Method for preparing medicine graded sustained-release bone repair body |
| CN102973334A (en) * | 2012-12-24 | 2013-03-20 | 天津大学 | Bionic design method of skull tissue engineering scaffold |
| CN104573218A (en) * | 2015-01-04 | 2015-04-29 | 秦晓亮 | Optimizing and fast modeling method for three-dimensional printing hole |
| CN105976425B (en) * | 2016-04-13 | 2019-12-03 | 深圳市艾科赛龙科技股份有限公司 | A kind of method and device of structure design |
| CN105976425A (en) * | 2016-04-13 | 2016-09-28 | 深圳市艾科赛龙科技有限公司 | Method and device for structural design |
| CN105997306A (en) * | 2016-04-25 | 2016-10-12 | 北京工业大学 | Design method for filling porous grid structure in bone implantation body |
| CN105997306B (en) * | 2016-04-25 | 2017-12-01 | 北京工业大学 | A kind of bone, which implants, fills the design method of perforated grill structure |
| CN105930617A (en) * | 2016-05-17 | 2016-09-07 | 南方医科大学 | Method for designing and forming stiffness-controllable bone tumor defect repair implant |
| CN106923936A (en) * | 2017-03-31 | 2017-07-07 | 中国人民解放军第四军医大学 | The design preparation method of the personalized customization 3D printing porous titanium alloy segmental prosthese rebuild for large segmental bone defect |
| CN107997855A (en) * | 2017-11-30 | 2018-05-08 | 深圳先进技术研究院 | 3D porous supports method for establishing model, device and preparation system |
| WO2020114253A1 (en) * | 2018-12-04 | 2020-06-11 | 北京市春立正达医疗器械股份有限公司 | Bone trabecula structure and prosthesis using same and manufacturing method therefor |
| CN113768665A (en) * | 2020-06-10 | 2021-12-10 | 上海交通大学医学院附属第九人民医院 | Bone defect repair support, construction method, preparation method, computer-readable storage medium and equipment |
| CN111899346A (en) * | 2020-07-16 | 2020-11-06 | 天津理工大学 | Mathematical modeling method of controllable porous bone tissue engineering scaffold |
| CN111899346B (en) * | 2020-07-16 | 2024-05-07 | 天津理工大学 | Mathematical modeling method of controllable porous bone tissue engineering scaffold |
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Application publication date: 20110223 |