CN101978974B - 一种蛋白核小球藻破壁提取生物活性物质的方法 - Google Patents

一种蛋白核小球藻破壁提取生物活性物质的方法 Download PDF

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CN101978974B
CN101978974B CN201010513417A CN201010513417A CN101978974B CN 101978974 B CN101978974 B CN 101978974B CN 201010513417 A CN201010513417 A CN 201010513417A CN 201010513417 A CN201010513417 A CN 201010513417A CN 101978974 B CN101978974 B CN 101978974B
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chlorella pyrenoidosa
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汤永强
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Dongguan morning Technology Co.,Ltd.
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Abstract

本发明公开了一种蛋白核小球藻破壁提取生物活性物质的方法，其包括以下步骤：A、将蛋白核小球藻活体浆液置入零下20～40℃温度冷冻，然后将冷冻得到的冰冻块放入容器内，采用物理挤压方法快速对冰冻块进行挤压，使冰冻块瞬间破碎和溶解；B、将挤压得到的破壁液放入搅拌器中搅拌；C、在温度为0～5℃的环境下，采用膜分离技术将破壁液富集得到生物活性物质。本发明对小球藻活体进行冰冻，使蛋白核小球藻细胞内的胞液对胞壁造成挤压，再采用物理挤压方法使冰冻块快速破裂。结合这两种手段能够充分使蛋白核小球藻的细胞壁破裂，使细胞壁内的胞液流出，从而有效提取蛋白核小球藻胞液内的生物活性物质。

Description

一种蛋白核小球藻破壁提取生物活性物质的方法
【技术领域】
本发明涉及生物技术领域，尤其涉及一种蛋白核小球藻破壁提取生物活性物质的方法。
【背景技术】
小球藻是一种高能量植物，其含有丰富的氨基酸、蛋白质及维生素等营养物质，尤其含有特有生物活性生长因子C.G.F(Chlorella Growth Facter)，具有很高的利用价值。
但是，目前还没有能够有效地提取小球藻中生物活性物质的方法。
【发明内容】
本发明针对现有技术的上述缺陷，提供一种蛋白核小球藻破壁提取生物活性物质的方法，以提取小球藻内生物活性物质。
为了解决上述技术问题，本发明是通过以下技术方案实现的：
一种蛋白核小球藻破壁提取生物活性物质的方法，其包括以下步骤：A、将蛋白核小球藻活体浆液置入零下20～40℃温度冷冻，然后将冷冻得到的冰冻块放入容器内，采用物理挤压方法快速对冰冻块进行挤压，使冰冻块瞬间破碎和溶解；B、将挤压得到的破壁液放入搅拌器中搅拌；C、在温度为0～5℃的环境下，采用膜分离技术将破壁液富集得到生物活性物质。
优选地，步骤A中采用100～150千克物体的重力对冰冻块进行挤压。
从以上技术方案可以看出，本发明对蛋白核小球藻活体进行冰冻，使蛋白核小球藻内的胞液冷冻膨胀对胞壁形成挤压，再采用物理挤压方法使冰冻块快速破裂，这两种手段结合后能够充分使小球藻的胞壁破裂，使胞液流出，从而有效提取蛋白核小球藻胞液内的生物活性物质。本发明整个提取过程均在低温下采用物理方法进行，不会对生物活性物质造成任何化学变化和影响，充分保证活性物质的活性。
【具体实施方式】
下面结合具体实施方式对本发明作进一步详细的说明。
本发明蛋白核小球藻破壁提取生物活性物质的方法包括以下步骤：
实施例一：首先将蛋白核小球藻活体浆液在零下30℃温度下进行冷冻得到冰冻块，然后将冰冻块放入容器中采用物理压力快速对冰冻块挤压，使冰冻块瞬间破碎，由于冰冻块受到快速冲击，冰冻块受热溶解得到破壁液；将得到的破壁液放入高速搅拌器使蛋白核小球藻细胞壁内的胞液充分溢出；然后在温度为2℃的环境下，采用膜分离技术将搅拌过后的破壁液富集得到生物活性物质。其中的膜分离技术为常规的现有技术，这里不作详细介绍。
实施例二：首先将蛋白核小球藻活体浆液在零下20℃温度下进行冷冻得到冰冻块，然后将冰冻块放入容器中采用150千克物体的重力快速对冰冻块进行挤压，使冰冻块瞬间破碎，由于冰冻块受到快速冲击，冰冻块受热溶解得到破壁液；将得到的破壁液放入高速搅拌器使蛋白核小球藻细胞壁内的胞液充分溢出；然后在温度为0℃的环境下，采用膜分离技术将搅拌过后的破壁液富集得到生物活性物质。
实施例三：首先将蛋白核小球藻活体浆液在零下40℃温度下进行冷冻得到冰冻块，然后将冰冻块放入容器中采用100千克物体的重力快速对冰冻块进行挤压，使冰冻块瞬间破碎和溶解得到破壁液；将破壁液放入高速搅拌器使蛋白核小球藻细胞壁内的胞液充分溢出；然后在温度为5℃的环境下，采用膜分离技术将搅拌过后的破壁液富集得到生物活性物质。
上述得到的生物活性物质可作为添加剂添加到药物当中，也可作为表皮速生长的药物，还可作为神经调节剂和保健用的免疫力调节营养液，具有非常高的应用价值。
上述蛋白核小球藻活体浆液是指蛋白核小球藻活体与培养液分离后得到的浆液。因为小球藻离开培养液后不能存活，所以必须存留一定量的培养液，因此称小球藻活体与培养液分离后的混合物为浆液。
以上所述实施例仅表达了本发明的几种实施方式，其描述较为具体和详细，但并不能因此而理解为对本发明专利范围的限制。应当指出的是，对于本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变形和改进，这些都属于本发明的保护范围。因此，本发明专利的保护范围应以所附权利要求为准。

Claims (2)

1.一种蛋白核小球藻破壁提取生物活性物质的方法，其特征在于，包括以下步骤：
A、将蛋白核小球藻活体浆液置入零下20～40℃温度冷冻，然后将冷冻得到的冰冻块放入容器内，采用物理挤压方法快速对冰冻块进行挤压，使冰冻块瞬间块破碎和溶解得到破壁液；
B、将挤压得到的破壁液放入搅拌器中搅拌；
C、在温度为0～5℃的环境下，采用膜分离技术将破壁液富集得到生物活性物质。
2.根据权利要求1所述的蛋白核小球藻破壁提取生物活性物质的方法，其特征在于，步骤A中采用100～150千克物体的重力对冰冻块进行挤压。
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CA2860968A1 (en) * 2012-01-11 2013-07-18 Sun Chlorella Corp. Use of cell wall disrupted product of chlorella for reducing serum resistin level
CN106821851B (zh) * 2017-02-20 2019-08-02 西安科艺诗生物技术有限公司 一种白梅花的鲜加工方法
CN107007646A (zh) * 2017-03-14 2017-08-04 郑笃达 一种辅助防治癌症的营养复合物
CN109480147A (zh) * 2018-11-27 2019-03-19 北海生巴达生物科技有限公司 利用浓缩及澄清技术制备饮料或螺旋藻生长因子粉的工艺
CN112110996A (zh) * 2020-10-29 2020-12-22 青岛科海生物有限公司 一种蛋白核小球藻中提取小球藻生长因子的方法

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