CN101978969A - Method for preparing propolis solution - Google Patents
Method for preparing propolis solution Download PDFInfo
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- CN101978969A CN101978969A CN2010105155320A CN201010515532A CN101978969A CN 101978969 A CN101978969 A CN 101978969A CN 2010105155320 A CN2010105155320 A CN 2010105155320A CN 201010515532 A CN201010515532 A CN 201010515532A CN 101978969 A CN101978969 A CN 101978969A
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- propolis
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- propolis extract
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Abstract
The invention relates to a method for preparing propolis solution, which comprises the following steps of: 1) crushing; 2) dissolving: adding a formula amount of solvent into a clean solution preparation tank, soaking the propolis extract crushed in the step 1) into the solvent, and stirring the mixture over night till full dissolution; or heating the mixture to 60 DEG C, stirring the mixture till full dissolution, standing the solution, cooling the solution to room temperature, and fixing the volume; 3) adjusting the pH value of the solution, namely measuring the pH value of the propolis extract solution, and adjusting the pH value to 7.0 by using a small amount of N,N-dimethyl acetamide; 4) suction filtering: performing suction filtering of the propolis extract solution by using a 0.8mu filter membrane; 5) packing: packing the propolis extract solution obtained in the step 4) into 100ml/bottle solution; and 6) sterilizing. The propolis extract is prepared into stable solution, and the solution is taken by drinking water or directly orally, or coated or sprayed on the affected part and used for improving the immunity of livestock and enhancing the disease resistance of the livestock.
Description
Technical field
The present invention relates to a kind of preparation method of propolis solution, be used to promote domestic animal immunity, strengthen the domestic animal premunition.
Background technology
Propolis derives from secreted natural gum of plant and resinae material, and after Apis is gathered, adds Cera Flava and the formed a kind of brown jelly of Apis maxillary secretions, is called as " purple gold ".Propolis contains hundreds of natural components.Mainly consisting of of fresh propolis: 50% resin and balsam, 30% Cera Flava, 10% fragrant volatile oil, 5% pollen, other materials (comprising organic acid, fatty acid, aminoacid, mineral element, vitamin, phytocide and enzyme etc.) of 5%.Propolis is a kind of natural active substance, and its composition is very complicated, contains flavonoid, terpenes, ketone, esters, alcohols, phenols, ethers, organic acid and a large amount of aminoacid, enzyme, VB class, VE and VA, and mineral etc.Propolis has biologic activity widely: (1) resisting pathogenic microbes activity: cause of diseases such as opposing antibacterial, virus, fungus, protozoon, nematicide participate in the body anti-infectious immunity; (2) toxin expelling activity: propolis can be got rid of the inside and outside toxin of body, protects kidney, protects the liver, cardiac stimulant effect; The activity of (3) inducing sweat: spasmolytic, calmness, antiinflammatory, pain relieving are arranged; (4) antioxidative activity: eliminate free radical, suppress the peroxidating of body inner lipid, improve resisting stress capability and to the adaptive capacity of envirment factor; (5) tissue repair activity: repair skin, mucosa, antiulcer, promotion tissue regeneration improve the body defence capability; (6) regulate immune activity: enhance immunity cytoactive, good immunological adjuvant and immunostimulant, immunity of two-ways regulation body cell and humoral immunization.
At present, propolis has been widely used in aspects such as medical and health, food industry, daily-use chemical industry, animal husbandry.Many national health departments approval propolis is health food or medicine, and propolis does not influence food safety, human body is had no side effect, and noresidue, the growth and the poultry that do not influence animal are laid eggs, even the trend of raising is arranged; The propolis product environmental protection.
Summary of the invention
The technical problem to be solved in the present invention is a kind of propolis solution of development, and this solution can be by domestic animal drinking-water or direct oral administration, and the affected part is smeared administration or through spray delivery, improved domestic animal immunity to reach, and strengthens the purpose of domestic animal premunition.
The preparation method of propolis solution of the present invention comprises the steps:
1), pulverizes: propolis extract is placed the plastic film bag of cleaning, break into pieces with hammer;
2), dissolving: add the solvent of prescription amount in the Agitation Tank of cleaning, the propolis extract that step 1) is pulverized soaks wherein, and stirring spends the night makes dissolving fully; Or be heated to 60 ℃, and be stirred to dissolving fully, leave standstill, be cooled to room temperature, standardize solution;
3), the solution pH value is regulated: measure the pH value of propolis extract solution, being adjusted to pH value with small amount of N N-dimethyl acetylamide is 7.0;
4), sucking filtration: with propolis extract solution through 0.8 μ m filter membrane sucking filtration;
5), packing: step 4) gained propolis extract solution is packed as 100ml/ bottle solution;
6), sterilization: the propolis extract solution that embedding is good is sterilized through autoclaving, and 1.0 * 105 handkerchief vapour pressures, were sterilized 15 minutes by 115 ℃.
The preparation method of propolis solution of the present invention, step 2) in, the solvent of described prescription amount is an alcohol solvent.
The preparation method of propolis solution of the present invention, step 2) in, the solvent of described prescription amount is propylene glycol, ethanol double solvents.
The preparation method of propolis solution of the present invention, step 2) in, the solvent of described prescription amount is propylene glycol, glyceryl triacetate double solvents.
The preparation method of propolis solution of the present invention, step 2) in, the solvent of described prescription amount is propylene glycol, ethanol, glyceryl triacetate double solvents.
Further, a kind of content specification of propolis solution, the content specification of propolis solution comprises 3 kinds: 5% propolis injection (g/v), 8% propolis injection (g/v) and 10% propolis injection (g/v).
Further, a kind of route of administration of propolis solution, the clinical practice of propolis solution can be by following approach:
(1) oral administration: domestic animal 0.2ml/kg;
(2) smear or spray delivery: be directly used in the affected part, cover the surface, affected part fully with Pharmaceutical.
The beneficial effect of the preparation method of propolis solution of the present invention is: preparation technology of the present invention is simple, and the Product Green environmental protection has no side effect.The present invention is prepared into all stable solution with propolis extract, by drinking-water or directly oral administration, smear in the affected part or spray delivery, is used to improve domestic animal immunity, strengthens the premunition of domestic animal.
The specific embodiment
Preparation method to propolis solution of the present invention is described further below.
With 100L, 10% propolis injection example:
Embodiment 1
The preparation method of propolis solution of the present invention comprises the steps:
1), pulverizes:, break into pieces with hammer with the plastic film bag that propolis extract 10kg places cleaning;
2), dissolving: add alcohol solvent in the Agitation Tank of cleaning, be settled to 100L, the propolis extract that step 1) is pulverized soaks wherein, and stirring spends the night makes dissolving fully; Or be heated to 60 ℃, and be stirred to dissolving fully, leave standstill, be cooled to room temperature, standardize solution;
3), the solution pH value is regulated: measure the pH value of propolis extract solution, being adjusted to pH value with small amount of N N-dimethyl acetylamide is 7.0;
4), sucking filtration: with propolis extract solution through 0.8 μ m filter membrane sucking filtration;
5), packing: step 4) gained propolis extract solution is packed as 100ml/ bottle solution;
6), sterilization: the propolis extract solution that embedding is good is sterilized through autoclaving, and 1.0 * 105 handkerchief vapour pressures, were sterilized 15 minutes by 115 ℃.
7), preserve: room temperature storage, expiry date are 2 years.
Propolis injection content specification involved in the present invention comprises: (1) 5% propolis solution (g/v); (2) 8% propolis solution (g/v); (3) 10% propolis solution (g/v).
Embodiment 2
Be with the embodiment difference: present embodiment step 2), adding prescription amount solvent in the Agitation Tank of cleaning is propylene glycol, ethanol double solvents, is settled to 100L.
Embodiment 3
Be with the embodiment difference: present embodiment step 2), adding prescription amount solvent in the Agitation Tank of cleaning is propylene glycol, glyceryl triacetate double solvents, is settled to 100L.
Embodiment 4
Be with the embodiment difference: present embodiment step 2), adding prescription amount solvent in the Agitation Tank of cleaning is ethanol, propylene glycol, glyceryl triacetate double solvents, is settled to 100L.
Write out a prescription applied single solvent and double solvents of propolis solution involved in the present invention all can quicken the dissolving of propolis extract, reduces the viscosity of propolis extract solution simultaneously, is more conducive to production practices.
The clinical practice of propolis solution involved in the present invention can be by following approach:
(1) oral administration: domestic animal 0.2ml/kg body weight
(2) smear or spray delivery: be directly used in the affected part, cover the surface, affected part fully with Pharmaceutical.
Above embodiment is described preferred implementation of the present invention; be not that scope of the present invention is limited; design under the prerequisite of spirit not breaking away from the present invention; various distortion and improvement that the common engineers and technicians in this area make technical scheme of the present invention all should fall in the definite protection domain of claims of the present invention.
Claims (7)
1. the preparation method of a propolis solution is characterized in that, comprises the steps:
1), pulverizes: propolis extract is placed the plastic film bag of cleaning, break into pieces with hammer;
2), dissolving: add the solvent of prescription amount in the Agitation Tank of cleaning, the propolis extract that step 1) is pulverized soaks wherein, and stirring spends the night makes dissolving fully; Or be heated to 60 ℃, and be stirred to dissolving fully, leave standstill, be cooled to room temperature, standardize solution;
3), the solution pH value is regulated: measure the pH value of propolis extract solution, being adjusted to pH value with small amount of N N-dimethyl acetylamide is 7.0;
4), sucking filtration: with propolis extract solution through 0.8 μ m filter membrane sucking filtration;
5), packing: step 4) gained propolis extract solution is packed as 100ml/ bottle solution;
6), sterilization: the propolis extract solution that embedding is good is sterilized through autoclaving, and 1.0 * 105 handkerchief vapour pressures, were sterilized 15 minutes by 115 ℃.
2. the preparation method of propolis solution according to claim 1 is characterized in that step 2) in, the solvent of described prescription amount is an alcohol solvent.
3. the preparation method of propolis solution according to claim 1 is characterized in that step 2) in, the solvent of described prescription amount is propylene glycol, ethanol double solvents.
4. the preparation method of propolis solution according to claim 1 is characterized in that step 2) in, the solvent of described prescription amount is propylene glycol, glyceryl triacetate double solvents.
5. the preparation method of propolis solution according to claim 1 is characterized in that step 2) in, the solvent of described prescription amount is propylene glycol, ethanol, glyceryl triacetate double solvents.
6. the content specification of a propolis solution is characterized in that, the content specification of propolis solution comprises 3 kinds: 5% propolis injection (g/v), 8% propolis injection (g/v) and 10% propolis injection (g/v).
7. the route of administration of a propolis solution is characterized in that, the clinical practice of propolis solution can be by following approach:
(1) oral administration: domestic animal 0.2ml/kg;
(2) smear or spray delivery: be directly used in the affected part, cover the surface, affected part fully with Pharmaceutical.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105155320A CN101978969A (en) | 2010-10-22 | 2010-10-22 | Method for preparing propolis solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105155320A CN101978969A (en) | 2010-10-22 | 2010-10-22 | Method for preparing propolis solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101978969A true CN101978969A (en) | 2011-02-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105155320A Pending CN101978969A (en) | 2010-10-22 | 2010-10-22 | Method for preparing propolis solution |
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| Country | Link |
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| CN (1) | CN101978969A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020251489A1 (en) * | 2019-06-13 | 2020-12-17 | Sbs Bi̇li̇msel Bi̇o Çözümler Sanayi̇ Ve Ti̇caret A.Ş. | Water-soluble and water-insoluble propolis products with high antioxidant capacity and their production methods |
| WO2021251920A1 (en) * | 2020-06-08 | 2021-12-16 | Sbs Bi̇li̇msel Bi̇o Çözümler Sanayi̇ Ve Ti̇caret A.Ş. | Use of propolis obtained by special extraction method in liquid mixtures |
| WO2021251918A1 (en) * | 2020-06-08 | 2021-12-16 | Sbs Bi̇li̇msel Bi̇o Çözümler Sanayi̇ Ve Ti̇caret A.Ş. | Use of propolis obtained by special extraction method in spreadable mixtures |
-
2010
- 2010-10-22 CN CN2010105155320A patent/CN101978969A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020251489A1 (en) * | 2019-06-13 | 2020-12-17 | Sbs Bi̇li̇msel Bi̇o Çözümler Sanayi̇ Ve Ti̇caret A.Ş. | Water-soluble and water-insoluble propolis products with high antioxidant capacity and their production methods |
| US11564952B2 (en) | 2019-06-13 | 2023-01-31 | Sbs Bilimsel Bio Çözümler Sanayi Ve Ticaret Anonim Sirketi | Water-soluble and water-insoluble propolis products with high antioxidant capacity and their production methods |
| WO2021251920A1 (en) * | 2020-06-08 | 2021-12-16 | Sbs Bi̇li̇msel Bi̇o Çözümler Sanayi̇ Ve Ti̇caret A.Ş. | Use of propolis obtained by special extraction method in liquid mixtures |
| WO2021251918A1 (en) * | 2020-06-08 | 2021-12-16 | Sbs Bi̇li̇msel Bi̇o Çözümler Sanayi̇ Ve Ti̇caret A.Ş. | Use of propolis obtained by special extraction method in spreadable mixtures |
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| C06 | Publication | ||
| DD01 | Delivery of document by public notice |
Addressee: Zhao Jinping Document name: Notification of Passing Preliminary Examination of the Application for Invention |
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| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20110223 |