CN101972449B - Chinese medicinal material earthworm enzyme and peptide compound preparation and preparation method thereof - Google Patents
Chinese medicinal material earthworm enzyme and peptide compound preparation and preparation method thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 14
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a Chinese medicinal material earthworm enzyme and peptide compound preparation and a preparation method thereof. The earthworm enzyme and peptide compound preparation is prepared by grinding Chinese medicinal herb plants into powder and mixing the powder with water and earthworms. In the method, the nerves of earthworms are suppressed by Chinese medicinal herbs instead of chemicals, so that a drastic stress reaction is not performed on the earthworms and clean active ingredients are slowly and gradually discharged from a body surface. Therefore, the problems of low yield of active ingredients, high product cost, poor product purity and the like in the prior art are solved.
Description
Technical field
The present invention relates to a kind of Chinese crude drug Lumbricus enzyme, peptide compound formulation and preparation method thereof, belong to field of pharmaceutical technology.
Background technology
Lumbricus is to belong to one type low firm no vertebra annelid such as grade of the few hair of Annelida, and body carefully is column, and whole body is divided into many joints.The still not multi-functional living resources of Lumbricus; Can rich soil; Environment purification along with the development of modern science and technology and going deep into of research, is found the highly active protein material that is rich in the multiple beneficial enzyme in the Lumbricus body fluid and is little peptide structure; The nutritional labeling such as abundant protein, aminoacid, vitamin, mineral element of animal and plant nutritional need can be provided, and Lumbricus also contains bioactive ingredients such as streptokinase, antioxidase, antibacterial peptide, antineoplastic component and hemolysin.Because these particular performances of Lumbricus and structure make its application very wide, can be production fields such as medicine, functional food, cosmetics, biological pesticide, bio-feritlizer, fine chemistry industry and develop new product safe, multi-purpose intermediate products are provided.But prior art generally adopts synthetic chemical material to extract as solvent, and then not only cost height and safety reduce, and range of application also will receive very big restriction.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Chinese crude drug Lumbricus enzyme, peptide compound formulation and preparation method thereof; It is neural that this method suppresses Lumbricus through adopting Chinese herbal medicine without chemicals; Make it not produce violent stress; Slowly and step by step discharge clean effective ingredient, thereby overcome the deficiency of prior art from body surface.
For solving the problems of the technologies described above, the present invention adopts following technical scheme: in a kind of Chinese crude drug Lumbricus enzyme, the peptide compound formulation: total amino acids content is 6～12%, total protein is: 8～15g/L, natural hormone are: gibberellins 70～100 μ g/mL, heteroauxing 12～40 μ g/mL, total humic acid are: 25～35mg/mL; Preparation is that solvent is prepared from the aqueous solution of Chinese herbal medicine medicine juice.The aqueous solution of so-called Chinese herbal medicine medicine juice is that Chinese herbal medicine is crushed into powder under the situation that has water to participate in that it dissolves the Chinese herbal juice that and constitutes the solvent that dissolves effective ingredient in the Lumbricus body fluid jointly with water.
In the previous formulations: total amino acids content: 8～9%; Total protein: 12～13g/L; Natural hormone: gibberellins 86～90 μ g/mL, heteroauxing 18～31 μ g/mL, total humic acid: 40～45mg/mL; And contain trace element: nitrogen 1～1.5g/L, manganese 0.06～0.1mg/L, phosphorus 0.1～0.15g/L, ferrum 1.0～1.4mg/L, potassium 0.2～0.25g/L, zinc 1.8～2mg/L, calcium 30～34.5mg/L, boron 0.25～0.3mg/L, magnesium 33～34mg/L, copper 0.35～0.4mg/L; PH value is 2～5.
In the previous formulations: total amino acids content: 8.6%; Total protein: 12.62g/L; Natural hormone: gibberellins 88 μ g/mL, heteroauxing 28 μ g/ml, total humic acid: 43mg/mL; And contain trace element: nitrogen 1.32g/L, manganese 0.09mg/L, phosphorus 0.14g/L, ferrum 1.04mg/L, potassium 0.24g/L, zinc 1.95mg/L, calcium 34.20mg/L, boron 0.27mg/L, magnesium 33.83mg/L, copper 0.39mg/L; PH value is 2～5.
The method for preparing of said Chinese crude drug Lumbricus enzyme, peptide compound formulation is: Chinese herbal medicine is clayed into power, is mixed with Lumbricus enzyme, peptide compound formulation with water, Lumbricus.
Aforementioned Chinese herbal medicine is: Mahonia fortunei (Lindl.) Fedde, Rhizoma Curcumae Longae and Pericarpium Zanthoxyli.
Aforesaid method for preparing comprises following step:
(1) Lumbricus that will live is cleaned with clean water, removes the foreign material that the Lumbricus body surface adheres to, and filters excessive moisture;
(2) be positioned in the rustless steel container after the Lumbricus of cleaning is weighed,, add the Mahonia fortunei (Lindl.) Fedde powder of its weight 3～6%, add the distilled water of 1.5～3 times of Lumbricus weight then, stirs 30～60 minutes, mistake and filter A liquid by the weight of Lumbricus;
(3) once more by Lumbricus weight, add the Turmeric Root of its weight 2～4%, add 1～2 times distilled water, stirred 20～30 minutes, cross and filter B liquid;
(4) repeat to add the Pricklyash peel of its weight 1～3%, add 1～2 times distilled water, stirred 30～50 minutes, cross and filter C liquid by Lumbricus weight;
(5) A, B and three liquid mixing of C are got mixed liquor, mixed liquor is positioned over 90～120 minutes after-filtration removals of contamination of water-bath sterilization in the rustless steel container, get D liquid;
(6) D liquid is packed into to leave standstill in the container of sealing get supernatant after 10～40 days and be enzyme, peptide compound formulation.
Aforesaid Mahonia fortunei (Lindl.) Fedde powder is through the Turmeric Root warp 80 mesh sieve blankings of 80 mesh sieve blankings, use, the Pricklyash peel warp 80 mesh sieve blankings of use.
In the aforesaid method, in preparation when A, B and three liquid of C, should be clockwise slowly stirring also all place rustless steel container to operate.
Employed medical material carries out the science compatibility and forms in the present technique, and it act as:
Mahonia fortunei (Lindl.) Fedde: slowly stimulate Lumbricus, utilize the organism of Lumbricus to shrink stress, pin each metabolism hole of Lumbricus body surface, make it not discharge dirt in the body.
Rhizoma Curcumae Longae: continue Lumbricus is produced calmness and inhibition and continues to discharge body fluid;
Pericarpium Zanthoxyli: the Lumbricus of inactivation is gradually produced necessary stimulation make it discharge intravital juice more up hill and dale;
The compatibility of three kinds of Chinese crude drugs and with the combining of Lumbricus effective ingredient; Improved the biological activity of enzyme peptide protein in this product greatly, strengthened for example antibiotic in the Lumbricus body fluid, antiinflammatory, antiviral, cytothesis, treatment acne, cardiovascular disease, blood pressure lowering, blood sugar lowering, blood fat reducing, the activation animal and plant growth factor, strengthen the animals and plants immunologic function, removed the function of beneficiating ingredients such as heavy-metal residual such as organophosphor; These three kinds of medicines suppress Lumbricus earlier and do not produce violent stress through scientific compatibility, after progressively remove and suppress, suitably increase then to stimulate and make it increase the speed and the quantity of discharge opeing; Effects such as the alkaloid in above-mentioned three kinds of Chinese crude drug compositions, curcumol, volatile plant oil, antioxidation have the positive transformation that melts to harmful components in the earthworm liquid; Dispel the inherent stink smell of earthworm liquid.
Thereby it is to consider under the least cost situation, to make the Chinese crude drug composition can more fully soak the purpose of Lumbricus being carried out enzymolysis that reaches soluble in water that present technique can be ground into 80 order powder with Chinese crude drug.80 orders are too low with the next active constituent content that soaks stripping, and then processing cost is too high more than 80 orders.
Compared with prior art, this method for preparing has effective component yield height, production cost is low, the product degree of purity is good advantage, and it is embodied in following aspect: insoluble matter is below 3%; Production cost is 1/4th of the same fermentoid of refabrication behind the synthetic chemical extraction agent extraction of the employing earthworm liquid, a peptide formulations; Compare with adopting synthetic chemical extraction agent patented technology, the enzyme peptide formulations of present technique preparation, its protein content exceeds 30%; Total amino acids content exceeds nearly four times; GA content exceeds 3 times; Total humic acid exceeds 2 times.The degree of purity of product depends on the stress of Lumbricus in extracting the earthworm liquid process, and dirt is too early then discharged in reaction; Reacted that then effective component yield is low slowly; The Chinese crude drug that present technique adopts has taken into full account Lumbricus and in certain period, has received effective ingredient that different stimulated then discharges and the relation between the quantity difference.
The specific embodiment
Embodiment 1:
(1) Lumbricus that will live is cleaned with clean water, removes the foreign material that the Lumbricus body surface adheres to, and filters excessive moisture;
(2) be positioned in the rustless steel container after the Lumbricus of cleaning is weighed,, add the Mahonia fortunei (Lindl.) Fedde powder of its weight 3%, add the distilled water of 1.5 times of Lumbricus weight then, stirs 30 minutes, mistake and filter A liquid by the weight of Lumbricus;
(3) once more by Lumbricus weight, add the Turmeric Root of its weight 2%, add 1 times distilled water, stirred 20 minutes, cross and filter B liquid;
(4) repeat to add the Pricklyash peel of its weight 1%, add 1 times distilled water, stirred 30 minutes, cross and filter C liquid by Lumbricus weight;
(5) A, B and three liquid mixing of C are got mixed liquor, stir clockwise, mixed liquor is positioned over 90 minutes after-filtration removals of contamination of water-bath sterilization in the rustless steel container, get D liquid;
(6) D liquid is packed into to leave standstill in the container of sealing get supernatant after 10 days and be enzyme, peptide compound formulation.
Embodiment 2:
(1) Lumbricus that will live is cleaned with clean water, removes the foreign material that the Lumbricus body surface adheres to, and filters excessive moisture;
(2) be positioned in the rustless steel container after the Lumbricus of cleaning is weighed,, add the Mahonia fortunei (Lindl.) Fedde powder of its weight 6%, add the distilled water of 3 times of Lumbricus weight then, stirs 60 minutes, mistake and filter A liquid by the weight of Lumbricus;
(3) once more by Lumbricus weight, add the Turmeric Root of its weight 4%, add 2 times distilled water, stirred 30 minutes, cross and filter B liquid;
(4) repeat to add the Pricklyash peel of its weight 3%, add 2 times distilled water, stirred 50 minutes, cross and filter C liquid by Lumbricus weight;
(5) A, B and three liquid mixing of C are got mixed liquor, stir clockwise, mixed liquor is positioned over 120 minutes after-filtration removals of contamination of water-bath sterilization in the rustless steel container, get D liquid;
(6) D liquid is packed into to leave standstill in the container of sealing get supernatant after 40 days and be enzyme, peptide compound formulation.
Embodiment 3:
(1) Lumbricus that will live is cleaned with clean water, removes the foreign material that the Lumbricus body surface adheres to, and filters excessive moisture;
(2) be positioned in the rustless steel container after the Lumbricus of cleaning is weighed,, add the Mahonia fortunei (Lindl.) Fedde powder of its weight 4%, add the distilled water of 2 times of Lumbricus weight then, stirs 40 minutes, mistake and filter A liquid by the weight of Lumbricus;
(3) once more by Lumbricus weight, add the Turmeric Root of its weight 3%, add 1.5 times distilled water, stirred 25 minutes, cross and filter B liquid;
(4) repeat to add the Pricklyash peel of its weight 2%, add 1.5 times distilled water, stirred 40 minutes, cross and filter C liquid by Lumbricus weight;
(5) A, B and three liquid mixing of C are got mixed liquor, mixed liquor is positioned over 100 minutes after-filtration removals of contamination of water-bath sterilization in the rustless steel container, get D liquid;
(6) D liquid is packed into to leave standstill in the container of sealing get supernatant after 20 days and be enzyme, peptide compound formulation.
Embodiment 4:
(1) Lumbricus that will live is cleaned with clean water, removes the foreign material that the Lumbricus body surface adheres to, and filters excessive moisture;
(2) be positioned in the rustless steel container after the Lumbricus of cleaning is weighed,, add the Mahonia fortunei (Lindl.) Fedde powder of its weight 5%, add the distilled water of 2.5 times of Lumbricus weight then, stirs 40 minutes, mistake and filter A liquid by the weight of Lumbricus;
(3) once more by Lumbricus weight, add the Turmeric Root of its weight 3.5%, add 1.75 times distilled water, stirred 28 minutes, cross and filter B liquid;
(4) repeat to add the Pricklyash peel of its weight 1.75%, add 2 times distilled water, stirred 45 minutes, cross and filter C liquid by Lumbricus weight;
(5) A, B and three liquid mixing of C are got mixed liquor, mixed liquor is positioned over 110 minutes after-filtration removals of contamination of water-bath sterilization in the rustless steel container, get D liquid;
(6) D liquid is packed into to leave standstill in the container of sealing get supernatant after 30 days and be enzyme, peptide compound formulation.
Embodiment 5:
(1) Lumbricus that will live is cleaned with clean water, removes the foreign material that the Lumbricus body surface adheres to, and filters excessive moisture;
(2) after being weighed, the Lumbricus of cleaning is positioned in the rustless steel container; By the weight of Lumbricus, add the Mahonia fortunei (Lindl.) Fedde powder (through 80 mesh sieve blankings) of its weight 5%, add the distilled water of 1.7 times of Lumbricus weight after 30 minutes; Slowly stirred 50 minutes clockwise, cross and filter A liquid;
(3) once more by Lumbricus weight, add the Turmeric Root (through 80 mesh sieve blankings) of its weight 3%, add 2 times distilled water after 25 minutes, slowly stirred 25 minutes clockwise, cross and filter B liquid;
(4) repeat by Lumbricus weight, add the Pricklyash peel (through 80 mesh sieve blankings) of its weight 1.5%, add 1 times distilled water after 35 minutes, slowly stirred 30 minutes clockwise, cross and filter C liquid;
(5) liquid mixing with A, B, C three phases gained gets D liquid, and D liquid is positioned over 100 minutes after-filtration removals of contamination of water-bath sterilization in the rustless steel container, crosses and filters C liquid;
(6) the D liquid behind the removal of contamination is packed into leave standstill that desirable supernatant is enzyme, peptide compound formulation after 30 days in the container of sealing.
More than in the Lumbricus enzyme that makes of each example, peptide compound formulation: total amino acids content is 6～12%, total protein is: 8～15g/L, natural hormone are: gibberellins 70～100 μ g/mL, heteroauxing 12～40 μ g/mL, total humic acid are: 25～35mg/mL.
Claims (3)
1. a Chinese crude drug Lumbricus enzyme, peptide compound formulation; It is characterized in that in the preparation: total amino acids content is 6～12%, total protein is: 8～15g/L, natural hormone are: gibberellins 70～100 μ g/mL, heteroauxing 12～40 μ g/mL, total humic acid are: 25～35mg/mL; Preparation is that solvent is prepared from the aqueous solution of Chinese herbal medicine medicine juice; Method for preparing is that Chinese herbal medicine is clayed into power, is mixed with Lumbricus enzyme, peptide compound formulation with water, Lumbricus; Said Chinese herbal medicine is: Mahonia fortunei (Lindl.) Fedde, Rhizoma Curcumae Longae and Pericarpium Zanthoxyli; Method for preparing comprises following step:
(1) Lumbricus that will live is cleaned with clean water, removes the foreign material that the Lumbricus body surface adheres to, and filters excessive moisture;
(2) be positioned in the rustless steel container after the Lumbricus of cleaning is weighed,, add the Mahonia fortunei (Lindl.) Fedde powder of its weight 3～6%, add the distilled water of 1.5～3 times of Lumbricus weight then, stirs 30～60 minutes, mistake and filter A liquid by the weight of Lumbricus;
(3) once more by Lumbricus weight, add the Turmeric Root of its weight 2～4%, add 1～2 times distilled water, stirred 20～30 minutes, cross and filter B liquid;
(4) repeat to add the Pricklyash peel of its weight 1～3%, add 1～2 times distilled water, stirred 30～50 minutes, cross and filter C liquid by Lumbricus weight;
(5) A, B and three liquid mixing of C are got mixed liquor, mixed liquor is positioned over 90～120 minutes after-filtration removals of contamination of water-bath sterilization in the rustless steel container, get D liquid;
(6) D liquid is packed into to leave standstill in the container of sealing get supernatant after 10～40 days and be enzyme, peptide compound formulation.
2. according to the described Chinese crude drug Lumbricus of claim 1 enzyme, peptide compound formulation, it is characterized in that: the Mahonia fortunei (Lindl.) Fedde powder of use is through the Turmeric Root warp 80 mesh sieve blankings of 80 mesh sieve blankings, use, the Pricklyash peel warp 80 mesh sieve blankings of use.
3. according to claim 1 or 2 said Chinese crude drug Lumbricus enzymes, peptide compound formulation, it is characterized in that: when preparation A, B and three liquid of C, should be clockwise slowly stirring also all place rustless steel container to operate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105158723A CN101972449B (en) | 2010-10-22 | 2010-10-22 | Chinese medicinal material earthworm enzyme and peptide compound preparation and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105158723A CN101972449B (en) | 2010-10-22 | 2010-10-22 | Chinese medicinal material earthworm enzyme and peptide compound preparation and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101972449A CN101972449A (en) | 2011-02-16 |
| CN101972449B true CN101972449B (en) | 2012-03-28 |
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| CN115944679A (en) * | 2022-12-14 | 2023-04-11 | 宏铖同创(山东)医药科技有限公司 | Chinese medicinal material earthworm enzyme and peptide compound preparation and preparation system and application thereof |
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| CN102188743A (en) * | 2011-05-13 | 2011-09-21 | 黄鹰 | Antibacterial and anti-inflammatory medical skin healing membrane and preparation method thereof |
| CN102265748A (en) * | 2011-05-23 | 2011-12-07 | 黄鹰 | Method for producing organic vegetables based on earthworm bioengineering technology |
| CN102274456B (en) * | 2011-07-22 | 2013-03-20 | 杨孟臻 | Pharmaceutical composition and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101356954A (en) * | 2008-08-30 | 2009-02-04 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of anti-stress medicine earthworm small-peptide chelated chromium |
| CN101570565A (en) * | 2008-04-30 | 2009-11-04 | 珠海博康药业有限公司 | Functional component prepared from earthworms for promoting growth of microorganisms and improving culture units and preparation method thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101570565A (en) * | 2008-04-30 | 2009-11-04 | 珠海博康药业有限公司 | Functional component prepared from earthworms for promoting growth of microorganisms and improving culture units and preparation method thereof |
| CN101356954A (en) * | 2008-08-30 | 2009-02-04 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of anti-stress medicine earthworm small-peptide chelated chromium |
Non-Patent Citations (2)
| Title |
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| 刘波等.蚯蚓蛋白酶解工艺及其产物分析.《上海水产大学学报》.2006,第15卷(第01期), * |
| 贾久满等.用蚯蚓制备复合氨基酸小肽螯合微量元素的研究.《饲料研究》.2009,(第07期), * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115944679A (en) * | 2022-12-14 | 2023-04-11 | 宏铖同创(山东)医药科技有限公司 | Chinese medicinal material earthworm enzyme and peptide compound preparation and preparation system and application thereof |
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