CN101570565A - Functional component prepared from earthworms for promoting growth of microorganisms and improving culture units and preparation method thereof - Google Patents
Functional component prepared from earthworms for promoting growth of microorganisms and improving culture units and preparation method thereof Download PDFInfo
- Publication number
- CN101570565A CN101570565A CNA200810027788XA CN200810027788A CN101570565A CN 101570565 A CN101570565 A CN 101570565A CN A200810027788X A CNA200810027788X A CN A200810027788XA CN 200810027788 A CN200810027788 A CN 200810027788A CN 101570565 A CN101570565 A CN 101570565A
- Authority
- CN
- China
- Prior art keywords
- earthworm
- preparation
- functional component
- column chromatography
- sephadex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention provides a functional component prepared from earthworms for promoting the growth of microorganisms and improving culture units and a preparation method thereof. The functional component is a mixture comprising multifunctional small earthworm peptides with the molecular weight of less than 21KD, polypeptides and growth promotion factors. The preparation method comprises the following steps: earthworm processing, homogenate preparing, centrifugal separation, ultrafiltration, column chromatography of molecular sieves, column chromatography of ion exchange, desalinization, low-temperature vacuum drying, and the like, and an obtained product is purified to obtain the functional component. The functional component is different from the prior microorganism growth factors such as vitamins, glutathione, butanediamine, and the like and is not a substitute of the microorganism growth factors. The functional component has stable property and high activity, can obviously promote the growth of the microorganisms and improve the culture units and ferment units and has wide use values and great social and economic significance in the fields of microorganism detection, microorganism culture, biological products, medicinal and industrial fermentation, and the like.
Description
Technical field
The invention provides a kind of from the promotion microorganism growth of earthworm preparation and functional component of raising culture units and preparation method thereof.This functional component is by the little peptide of the multiple function of the earthworm of molecular weight below 21KD, the mixture that polypeptide and somatomedin are formed.Microorganism growth be can significantly promote and cultivation and fermentation unit improved.Its preparation method comprises that steps such as earthworm processing, homogenate, centrifugation, ultrafiltration, molecular sieve column chromatography, ion-exchange chromatography, desalination, low-temperature vacuum drying carry out purifying and obtain.At Micro biological Tests, microorganism culturing, biological products, there are use value and important social economic implications widely in fields such as medicine and industrial fermentation.
Background technology
In various microbiological culture medias commonly used, add the functional component of the present invention's preparation, can significantly promote microorganism growth; In the microorganism culturing and fermentation culture of medicine industry, biological products industry, add the functional component of the present invention's preparation, can significantly improve microorganism culturing and fermentation culture unit.This multicomponent mixture by the little peptide of multiple function, polypeptide and the somatomedin composition of molecular weight below 21KD from the earthworm preparation, be different from the microorganism growth factors such as known VITAMIN, gsh, butanediamine, neither its substitute, do not report, be the functional component that a kind of new having promotes microorganism growth and improve culture units before.
Summary of the invention
The invention provides a kind of from the promotion microorganism growth of earthworm preparation and functional component of raising culture units and preparation method thereof.Its earthworm, comprise propagate artificially, wild, fresh and alive or drying earthworm is a material.This functional component is by the little peptide of the multiple function of the earthworm of molecular weight below 21KD, the mixture that polypeptide and somatomedin are formed.Its preparation technology's method is as follows:
(1) preparation of earthworm crude extract.Earthworm is washed, add the tap water of 10-50%, the hydrochloric acid of PH=4-6 or phosphoric acid solution 2-10%, normal temperature rubs homogenate down, and under 35-45 ℃ of temperature, earthworm body self enzymolysis becomes earthworm extract.
(2) with the earthworm liquid centrifugation.
(3) get supernatant liquor, with getting the earthworm crude extract by the ultrafiltration system filtering separation of molecular weight 21000Da specification.
(4) separation and purification.A: molecular sieve column chromatography, filler: Sephadex G-250; Last sample: earthworm crude extract; Elutriant: 0.030-0.035M acetate, pH3-6; UV256nm detects, and presses absorption peak and collects.
B: ion-exchange chromatography, filler: DEAE Mierocrystalline cellulose DE125; Balance liquid: 0.003-0.006M Tris-HCL, PH6.0-8.5; Gradient elution; Last sample: Sephadex G-250 column chromatography, with going up sample behind balance liquid and the dissolve with ethanol.
(5) desalination.Filler: Sephadex G-120; Elutriant: 0.030-0.035M acetate, PH3-6; Detect wavelength 256nm.
(6) solution of collecting is carried out low-temperature vacuum drying promptly.
This multi-component functional component of molecular weight below 21KD from the earthworm preparation can significantly promote microorganism growth and improve culture units.It has the function that promotes microorganism growth and improve culture units, but is different from the microorganism growth factors such as known VITAMIN, gsh, butanediamine, neither its substitute.On the basis of using somatomedin compositions such as present known VITAMIN, product provided by the invention can significantly promote microorganism growth and improve culture units.At Micro biological Tests, microorganism culturing, biological products, there are use value and social and economic significance widely in medicine and industrial fermentation field.
Embodiment
Embodiment one
(1) preparation of earthworm crude extract.Fresh and alive earthworm is washed, add the tap water of 10-20%, the hydrochloric acid of PH=4-6 or phosphoric acid solution 2-5%, normal temperature rubs homogenate down, and under 35-45 ℃ of temperature, earthworm body self enzymolysis becomes earthworm extract.
(2) with the earthworm liquid centrifugation.
(3) get supernatant liquor, with getting the earthworm crude extract by the ultrafiltration system filtering separation of molecular weight 21000Da specification.
(4) separation and purification.A: molecular sieve column chromatography, filler: Sephadex G-250; Last sample: earthworm crude extract; Elutriant: 0.030-0.035M acetate, PH3-6; UV256nm detects, and presses absorption peak and collects.
B: ion-exchange chromatography, filler: DEAE Mierocrystalline cellulose DE125; Balance liquid: 0.003-0.006M Tris-HCL, PH6.0-8.5; Gradient elution; Last sample: Sephadex G-250 column chromatography, with going up sample behind balance liquid and the dissolve with ethanol.
(5) desalination.Filler: Sephadex G-120; Elutriant: 0.030-0.035M acetate, PH3-6; Detect wavelength 256nm.
(6) solution of collecting is carried out low-temperature vacuum drying promptly.
Embodiment two
(1) preparation of earthworm crude extract.Air-dry earthworm is washed, add the tap water of 30-50%, the hydrochloric acid of PH=4-6 or phosphoric acid solution 5-10%, normal temperature rubs homogenate down, and under 35-45 ℃ of temperature, earthworm body self enzymolysis becomes earthworm extract.
(2) with the earthworm liquid centrifugation.
(3) get supernatant liquor, with getting the earthworm crude extract by the ultrafiltration system filtering separation of molecular weight 21000Da specification.
(4) separation and purification.A: molecular sieve column chromatography, filler: Sephadex G-250; Last sample: earthworm crude extract; Elutriant: 0.030-0.035M acetate, PH3-6; UV256nm detects, and presses absorption peak and collects.
B: ion-exchange chromatography, filler: DEAE Mierocrystalline cellulose DE125; Balance liquid: 0.003-0.006M Tris-HCL, PH6.0-8.5; Gradient elution; Last sample: Sephadex G-250 column chromatography is gone up sample after with balance liquid and dissolve with ethanol.
(5) desalination.Filler: Sephadex G-120; Elutriant: 0.030-0.035M acetate, PH3-6; Detect wavelength 256nm.
(6) solution of collecting is carried out low-temperature vacuum drying promptly.
Claims (3)
1, a kind of from the promotion microorganism growth of earthworm preparation and functional component of raising culture units and preparation method thereof.This functional component is by the little peptide of the multiple function of the earthworm of molecular weight below 21KD, the mixture that polypeptide and somatomedin are formed.Its preparation technology's method is as follows:
(1) preparation of earthworm crude extract.Earthworm is washed, add the tap water of 10-50%, the hydrochloric acid of PH=4-6 or phosphoric acid solution, normal temperature rubs homogenate down, and under 35-42 ℃ of temperature, earthworm body self enzymolysis becomes earthworm extract.
(2) with the earthworm liquid centrifugation.
(3) get supernatant liquor, with after the acetate buffer dilution, with can filter by the ultrafiltration system of molecular weight 21000Da specification or supercentrifuge separate the earthworm crude extract.
(4) separation and purification.A: molecular sieve column chromatography, filler: Sephadex G-250; Last sample: earthworm crude extract; Elutriant: 0.030-0.035M acetate, PH3-6; UV256nm detects, and presses absorption peak and collects.B: ion-exchange chromatography, filler: DEAE Mierocrystalline cellulose DE 125; Balance liquid: 0.003-0.006MTris-HCL, PH6.0-8.5; Gradient elution; Last sample: Sephadex G-250 column chromatography, with going up sample behind balance liquid and the dissolve with ethanol.
(5) desalination, filler: Sephadex G-120; Elutriant: 0.030-0.035M acetate, pH3-6; Detect wavelength 256nm.
(6) solution of collecting is carried out low-temperature vacuum drying promptly.
2, according to claim 1 from the promotion microorganism growth of earthworm preparation and functional component of raising culture units and preparation method thereof, it is characterized in that this functional component is by the little peptide of the multiple function of the earthworm of molecular weight below 21KD, polypeptide and somatomedin composition.
3, according to claim 1 from the promotion microorganism growth of earthworm preparation and functional component of raising culture units and preparation method thereof, it is characterized in that the little peptide of the multiple function of earthworm, polypeptide and somatomedin separation and purification be: A: molecular sieve column chromatography, filler are Sephadex G-250; Last sample: earthworm crude extract; Elutriant is a 0.030-0.035M acetate, PH=3-6.B: ion-exchange chromatography, filler are DEAE Mierocrystalline cellulose DE 125; Balance liquid is 0.003-0.006MTris-HCL, PH=6.0-8.5; Gradient elution; Last sample is a Sephadex G-250 column chromatography.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200810027788XA CN101570565A (en) | 2008-04-30 | 2008-04-30 | Functional component prepared from earthworms for promoting growth of microorganisms and improving culture units and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200810027788XA CN101570565A (en) | 2008-04-30 | 2008-04-30 | Functional component prepared from earthworms for promoting growth of microorganisms and improving culture units and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101570565A true CN101570565A (en) | 2009-11-04 |
Family
ID=41230060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200810027788XA Pending CN101570565A (en) | 2008-04-30 | 2008-04-30 | Functional component prepared from earthworms for promoting growth of microorganisms and improving culture units and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101570565A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101972449A (en) * | 2010-10-22 | 2011-02-16 | 杨孟臻 | Chinese medicinal material earthworm enzyme and peptide compound preparation and preparation method thereof |
| CN103114111A (en) * | 2012-11-14 | 2013-05-22 | 江苏江山制药有限公司 | Processing method for enhancing fermentation productivity of L-sorbose |
| CN104593456A (en) * | 2014-12-02 | 2015-05-06 | 高向阳 | Method for preparing peptone from enzymatically-hydrolyzed earthworm dry powder and purpose of peptone |
| CN106619729A (en) * | 2016-09-27 | 2017-05-10 | 陈雨 | Preparation method for earthworm extract solution |
| CN106928314A (en) * | 2015-12-29 | 2017-07-07 | 江苏步龙生物科技有限公司 | A kind of method that earthworm polypeptide is extracted from earthworm |
| CN109260231A (en) * | 2017-07-18 | 2019-01-25 | 首都儿科研究所 | The preparation method of the anti-inflammatory antimicrobial extract of cough-relieving apophlegmatic in a kind of earthworm |
| CN112375795A (en) * | 2020-10-23 | 2021-02-19 | 内蒙古拜克生物有限公司 | Method for producing acetylglucosamine by microbial fermentation |
-
2008
- 2008-04-30 CN CNA200810027788XA patent/CN101570565A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101972449A (en) * | 2010-10-22 | 2011-02-16 | 杨孟臻 | Chinese medicinal material earthworm enzyme and peptide compound preparation and preparation method thereof |
| CN101972449B (en) * | 2010-10-22 | 2012-03-28 | 杨孟臻 | Chinese medicinal material earthworm enzyme and peptide compound preparation and preparation method thereof |
| CN103114111A (en) * | 2012-11-14 | 2013-05-22 | 江苏江山制药有限公司 | Processing method for enhancing fermentation productivity of L-sorbose |
| CN105018543A (en) * | 2012-11-14 | 2015-11-04 | 帝斯曼江山制药（江苏）有限公司 | Processing method for enhancing fermentation productivity of L-sorbose |
| CN104593456A (en) * | 2014-12-02 | 2015-05-06 | 高向阳 | Method for preparing peptone from enzymatically-hydrolyzed earthworm dry powder and purpose of peptone |
| CN106928314A (en) * | 2015-12-29 | 2017-07-07 | 江苏步龙生物科技有限公司 | A kind of method that earthworm polypeptide is extracted from earthworm |
| CN106619729A (en) * | 2016-09-27 | 2017-05-10 | 陈雨 | Preparation method for earthworm extract solution |
| CN109260231A (en) * | 2017-07-18 | 2019-01-25 | 首都儿科研究所 | The preparation method of the anti-inflammatory antimicrobial extract of cough-relieving apophlegmatic in a kind of earthworm |
| CN109260231B (en) * | 2017-07-18 | 2021-09-03 | 首都儿科研究所 | Preparation method of earthworm extract with cough stopping, phlegm eliminating, anti-inflammatory and antimicrobial functions |
| CN112375795A (en) * | 2020-10-23 | 2021-02-19 | 内蒙古拜克生物有限公司 | Method for producing acetylglucosamine by microbial fermentation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhao et al. | Purification and characterization of a novel lectin from the toxic wild mushroom Inocybe umbrinella | |
| CN101570565A (en) | Functional component prepared from earthworms for promoting growth of microorganisms and improving culture units and preparation method thereof | |
| CN104250285B (en) | Pseudosciaena crocea flesh antioxidative peptide and preparation method and use thereof | |
| WO2015103974A1 (en) | Method for extracting and purifying l-ergothioneine | |
| CN107312807A (en) | The enzymolysis preparation of the brown alga function oligosaccharides in one main laminaria source | |
| CN103073652A (en) | Method for extracting polysaccharide of spirulina platensis | |
| CN104250286B (en) | Navodon septentrionalis fish-skin antioxidant collagen peptide and preparation method and use thereof | |
| CN113186242A (en) | Preparation method and application of distillers' grain alcohol-soluble peptide | |
| CN104558115A (en) | Antioxidant polypeptide with Raja porosa meat protein as well as preparation method and application of antioxidant polypeptide | |
| CN104774827A (en) | Method for preparing alginate lyase from abalone internal organs | |
| CN110256597B (en) | Method for reducing heavy metal residues in ganoderma lucidum polysaccharide by membrane method | |
| CN112245342A (en) | Ginseng series skin care extract and preparation method and application thereof | |
| CN104892730B (en) | Hairtail liver antibacterial peptide | |
| CN104177484A (en) | Prostatic cancer-resistant mussel glycoprotein and preparation and application thereof | |
| CN113444162B (en) | Small peptide with anti-inflammatory activity, preparation method and application thereof | |
| CN111825745B (en) | Immunoregulatory active heptapeptide and preparation method and application thereof | |
| CN108530557B (en) | Laminarin extraction and purification process and application | |
| KR101346059B1 (en) | The obtaining method of hydrolysate from silkworm gland | |
| CN110655554B (en) | Protein active peptide for improving saccharomyces cerevisiae proliferation and ethanol tolerance as well as preparation method and application thereof | |
| JP2022517218A (en) | Methods for extracting phycocyanin | |
| CN104892723B (en) | Preparation method of hairtail liver antibacterial peptide | |
| AU2021104232A4 (en) | Application of sanghuangporus sanghuang fermentation broth polysaccharides in medicaments of anti-avian influenza virus | |
| CA3100869A1 (en) | Method for preparing precursor of recombinant human insulin or analogue thereof | |
| CN114763370B (en) | Antioxidant decapeptide and preparation method and application thereof | |
| CN111978374B (en) | Immunoregulation active hexapeptide and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| DD01 | Delivery of document by public notice |
Addressee: Zhuhai Bokang Pharmaceutical Industry Co., Ltd. Document name: Notification of Patent Invention Entering into Substantive Examination Stage |
|
| DD01 | Delivery of document by public notice |
Addressee: Zhuhai Bokang Pharmaceutical Industry Co., Ltd. Zhong Shiyong Document name: the First Notification of an Office Action |
|
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20091104 |