CN101966254A - Application of raw rehmannia aqueous extract in preparing medicines for treating woman climacteric syndrome caused by hyposecretion of in-vivo female hormones - Google Patents
Application of raw rehmannia aqueous extract in preparing medicines for treating woman climacteric syndrome caused by hyposecretion of in-vivo female hormones Download PDFInfo
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- CN101966254A CN101966254A CN2010102989960A CN201010298996A CN101966254A CN 101966254 A CN101966254 A CN 101966254A CN 2010102989960 A CN2010102989960 A CN 2010102989960A CN 201010298996 A CN201010298996 A CN 201010298996A CN 101966254 A CN101966254 A CN 101966254A
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- hyposecretion
- climacteric syndrome
- aqueous extract
- syndrome caused
- preparing medicines
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Abstract
The invention relates to an application of a raw rehmannia aqueous extract in preparing medicines for treating woman climacteric syndrome caused by hyposecretion of in-vivo female hormones. The invention can effectively solve the problem in preparing medicines for treating woman climacteric syndrome caused by hyposecretion of in-vivo female hormones. The technical scheme comprises the following steps: adding water to raw rehmannia to decoct for 2 hours twice, wherein the water added for each time is 10 times of raw rehmannia by weight; and merging the two water decoction solutions, depressurizing, concentrating and drying to obtain the raw rehmannia paste aqueous extract. The invention can be effectively used for preparing medicines for treating woman climacteric syndrome caused by hyposecretion of in-vivo female hormones. The preparation method of the aqueous extract is simple, stable, reliable, convenient and cheap, and obtains the same or similar result after the preparation is carried out many times. The aqueous extract can be effectively used for preparing medicines for treating woman climacteric syndrome caused by hyposecretion of in-vivo female hormones. The invention develops a new medicinal application for raw rehmannia, and has significant clinical meaning.
Description
One, technical field
The present invention relates to medicine, the application in particularly a kind of Radix Rehmanniae water extract climacteric syndrome medicine that the estrogen hyposecretion causes in preparation is treated owing to body.
Two, background technology
Radix Rehmanniae is the fresh or dry tuber of scrophulariaceae rehmannia glutinosa plant (Rehmannia glutinosa Libosch.).Excavate autumn, removes removing LU, fibrous root and silt, it slowly cured to about eighty per cant do, and is Radix Rehmanniae.The successive dynasties famous expert all has argumentation and research to it, thinks that its property is sweet, cold, GUIXIN, kidney, Liver Channel, clearing away heat and cooling blood, YIN nourishing and the production of body fluid promoting.Cure mainly that calentura crimson tongue excessive thirst, interior-heat caused by deficiency of YIN, hectic fever due to YIN-deficiency consumptive fever, interior-heat are quenched one's thirst, haematemesis, epistaxis, metrorrhagia, menoxenia, frequent fetal movement, the moon hinder constipation etc.Modern pharmacology and clinical research show that Radix Rehmanniae can promote blood coagulation, rising peripheral leukocytes; Energy heart tonifying, diuresis, rising blood pressure; The energy the liver protecting, blood sugar lowering; Raise immunity, Antiradiation injury and the effect of adrenocortical hormone sample are arranged.But the research report of Radix Rehmanniae about the active aspect of phytoestrogen do not arranged as yet.
Three, summary of the invention
At above-mentioned situation, the present invention's purpose just provides the application in a kind of Radix Rehmanniae water extract climacteric syndrome medicine that the estrogen hyposecretion causes in preparation is treated owing to body, can effectively solve the medication problem that the climacteric syndrome that causes owing to estrogen hyposecretion in the body is treated in preparation.
The technical scheme that the present invention solves is, the water that Radix Rehmanniae is added at every turn its 10 times of weight, decoct twice, each 2 hours, merge decocting liquid twice, decompression, concentrated, dry gets Radix Rehmanniae extractum shape water extract, is effective to prepare the medicine for the treatment of the climacteric syndrome that causes owing to estrogen hyposecretion in the body.
Water extract preparation method of the present invention is simple, reliable and stable, through repeatedly (more than 10 times) preparation, all obtained identical or akin result, convenient, cost is low, and its water extract is effective to prepare the climacteric syndrome medicine that treatment causes owing to estrogen hyposecretion in the body, opened up the medicinal new purposes of Radix Rehmanniae, clinical meaning is huge.
Four, the specific embodiment
The specific embodiment of the present invention is elaborated with relevant testing data below in conjunction with embodiment.
At first choose fresh or dry scrophulariaceae rehmannia glutinosa plant (the Rehmannia glutinosa Libosch.) tuber of excavating autumn, remove removing LU, fibrous root and silt, it is slowly cured to about eighty per cant do, be Radix Rehmanniae, choose Radix Rehmanniae 100g then, add entry 1000g (or 1000ml) at every turn, decoct 2 times, each 2 hours, merge 2 times decocting liquid, decompression, concentrated, dry gets Radix Rehmanniae extractum shape water extract 71.90g; Water extract is effective to prepare the climacteric syndrome medicine that treatment causes owing to estrogen hyposecretion in the body, and has obtained fully proving through animal experiment, and its relevant testing data is as follows:
By MCF-7 cell proliferation experiment (E-SCREEN), prove that Radix Rehmanniae can promote the MCF-7 cell proliferation, illustrate that it has an estrogen-like effects external.
By Mouse Uterus weightening finish experiment, the checking Radix Rehmanniae can increase the uterus coefficient of sex immature mice, illustrates that it has estrogen-like effects in vivo.
Proved the new purposes of water extract of the present invention by experiment at preparation estrogen medicine.
Concrete experiment material and method are:
1. the preparation of medicine
Fresh or the dry tuber of scrophulariaceae rehmannia glutinosa plant (Rehmannia glutinosa Libosch.).Excavate autumn, removes removing LU, fibrous root and silt, it slowly cured to about eighty per cant do, and is Radix Rehmanniae.
Radix Rehmanniae 100g boils 2 times with the decocting of 10 times of amounts, each 2h, and decocting liquid drying under reduced pressure concentrate drying obtains Radix Rehmanniae water extract 71.90g (extractum shape).
2. laboratory animal and cell strain
Kunming mouse, female, be born 21 days (ablactation just).Body weight 9～12g purchases the Experimental Animal Center in Henan Province.Human breast cancer cell (MCF-7) is provided by Chinese BIO ENGINEERING INST MILITARY.
3. main agents
(Newborn Calf Serum is NCS) available from GibcoInvitrogen company for RPMI1640 culture medium, calf serum; Do not have phenol red RPMI1640 culture medium, 17 beta estradiols (17 β-estrogen, E2), active carbon (Charcoal) is all available from Sigma company; Glucosan T-70 (Dextran-70) is available from Shanghai chemical reagents corporation; Synestrin tablets (Hefei joins pharmacy for a long time).
4. used key instrument
Electronical reading analytical balance (Japanese Shimadzu company); The general surgery apparatus; CO2 gas incubator (Japanese SANYO company); Inverted microscope (Japanese Olympus); KDC-160HR high-speed low temperature refrigerated centrifuger (Keda Innovation Co., Ltd); Microplate reader (BIO-RAD 680).
5. experimental technique
(1) Mouse Uterus weightening finish experiment
Mice is balanced by body weight and principle is at random divided into groups.Administration continues 7 days.Blank group distilled water every day 0.2ml.10g
-1Irritate stomach; Positive controls diethylstilbestrol every day suspension (0.035mgkg
-1D
-1) the filling stomach; Each administration group of Radix Rehmanniae is calculated by 20 times of amount * extraction ratios of clinical application, is made into the suspension of respective concentration, presses 0.02ml/10g every day and irritates stomach filling stomach.Behind the last administration 24h, take off neck and put to death mice, win the uterus immediately and weigh, calculate uterus coefficient (uterus weight in wet base (body weight)
-1100%).Test parallel triplicate.The result compares with the blank group, and calculating the investigation of P value has not statistically significant.
(2) MCF-7 cell proliferation experiment
The MCF-7 cell is chosen the exponential phase cell after not having phenol red 1,640 2 weeks of cultivation, PBS washes twice, behind 0.25% trypsinization, adds serum-free 1640 piping and druming evenly, with 2 * 10
3The concentration in individual/hole is inoculated in 96 orifice plates, and it is 200 μ l that cumulative volume is cultivated in every hole.Cultivate treated cell attachment in 24 hours after, be changed to the pastille culture fluid and continue cultivate.37 ℃, 5%CO
2Cultivate after 3 days, every hole adds MTT solution (5mg/ml) 20 μ l, and 37 ℃ are continued to cultivate 4 hours, the culture fluid that carefully exhausts, and every hole adds 150 μ lDMSO, shakes 5～10 minutes, and crystal is dissolved fully.With the DMSO zeroing, under 490nm, measure each hole absorbance (A) with microplate reader, the calculating average A value and the rate of increase (Proliferation Rate, RP).Test parallel triplicate.
RP=(experimental group A value/blank group A value)-1 * 100%
6. statistical disposition
Experimental data with
Expression, SPSS13.0 carries out one factor analysis of variance (One-Way ANOVA) statistical disposition.
Experimental result
One, adopts human breast carcinoma MCF-7 cell proliferation experiment in conjunction with zoopery, the Radix Rehmanniae water extract is carried out the experiment of estrogen activity screening
1. the Radix Rehmanniae water extract is to the experimental result that influences of the propagation of MCF-7 cell
The result shows that Radix Rehmanniae is at 0.001～0.1mgml
-1The time to MCF-7 cell and blank group than short extremely significantly proliferation function (P＜0.01) is all arranged, and be dose dependent.See Table 1.Explanation is in experiment in vitro, and Radix Rehmanniae shows as estrogen-like effects when low concentration.
Table 1 Radix Rehmanniae is to the influence of MCF-7 cell proliferation
Annotate with the blank group and compare, ★ represents P＜0.05, and ☆ represents P＜0.01; Compare with positive controls, ▲ expression P＜0.05, △ represents P＜0.01.
2. Radix Rehmanniae water extract Mouse Uterus weightening finish experimental result
The result shows that Radix Rehmanniae water extract group Mouse Uterus coefficient is compared with the blank group all has utmost point significant difference (P＜0.01). illustrate that the Radix Rehmanniae water extract has estrogen activity in the mice body.See Table 2.
Table 2 Radix Rehmanniae is to the influence of sex immature Mouse Uterus coefficient
Annotate with the blank group and compare, ★ represents P＜0.05, and ☆ represents P＜0.01; Compare with positive controls, ▲ expression P＜0.05, △ represents P＜0.01.
Two, conclusion
The MCF-7 cell is the male human breast cancer cell strain of estrogen receptor (ER), can be subjected to estrogen or estrogenic activity material to regulate and breed specifically, is the cell strain of the most frequently used detection estrogenic activity.MCF-7 cell estrogen-like proliferation test is very sensitive, can distinguish estrogen agonist and antagonist, thereby is widely used in external a large amount of, rapid screening and evaluation environmental estrogens and phytoestrogen.In the experiment of Radix Rehmanniae to the MCF-7 impact cell, Radix Rehmanniae all has significant proliferation function to MCF-7 when 0.001mg/ml～0.1mg/ml, and the result shows that Radix Rehmanniae has estrogen-like effects external, and the power of effect has dose dependent.
Above-mentioned test is carried out repeatedly repeatedly, in two years different time carried out 30 surplus time, all obtained identical or close result.
Uterus weight experiment is the classical way of the detection estrogen activity set up the earliest, when estimating estrogen activity, it has considered the absorption, metabolism of material, combine with plasma protein and many-sided factor such as pharmacokinetics, and directly reflection is subjected to the overall biological effect of reagent thing behind internal metabolism.Therefore, this experiment adopts the ratio (uterus coefficient) of property end adult female Mouse Uterus weight in wet base and body weight as the index of estimating estrogen activity.The result shows, Radix Rehmanniae crude drug water extract can significantly increase the Mouse Uterus coefficient, proved that Radix Rehmanniae has estrogen-like effects in vivo, can be effective to prepare the medicine for the treatment of the climacteric syndrome that causes owing to estrogen hyposecretion in the body, be that one on the Chinese medicine purposes innovated greatly.
Claims (1)
- A Radix Rehmanniae water extract the preparation treatment owing to body in application in the climacteric syndrome medicine that causes of estrogen hyposecretion, said water extract is each water that adds 10 times of weight of Radix Rehmanniae, decoct twice, each 2 hours, merge decocting liquid twice, decompression, concentrated, dry gets Radix Rehmanniae extractum shape water extract.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102989960A CN101966254A (en) | 2010-10-08 | 2010-10-08 | Application of raw rehmannia aqueous extract in preparing medicines for treating woman climacteric syndrome caused by hyposecretion of in-vivo female hormones |
| CN 201110211181 CN102247471B (en) | 2010-10-08 | 2011-07-26 | Application of rehmannia root water extract to preparing estrogen medicament |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102989960A CN101966254A (en) | 2010-10-08 | 2010-10-08 | Application of raw rehmannia aqueous extract in preparing medicines for treating woman climacteric syndrome caused by hyposecretion of in-vivo female hormones |
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| CN101966254A true CN101966254A (en) | 2011-02-09 |
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| CN 201110211181 Active CN102247471B (en) | 2010-10-08 | 2011-07-26 | Application of rehmannia root water extract to preparing estrogen medicament |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102247471A (en) * | 2010-10-08 | 2011-11-23 | 河南中医学院 | Application of rehmannia root water extract to preparing estrogen medicament |
| CN102579673A (en) * | 2012-03-15 | 2012-07-18 | 河南中医学院 | Application of fresh rehmannia root aqueous extract in preparation of estrogen medicines |
| CN110946921A (en) * | 2019-11-21 | 2020-04-03 | 张家港市中医医院 | Traditional Chinese medicine for relieving side effects of glucocorticoid medicaments |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004155779A (en) * | 2002-10-16 | 2004-06-03 | Taisho Pharmaceut Co Ltd | Peroral composition for preventing and treating climacteric symptom |
| CN101966254A (en) * | 2010-10-08 | 2011-02-09 | 河南中医学院 | Application of raw rehmannia aqueous extract in preparing medicines for treating woman climacteric syndrome caused by hyposecretion of in-vivo female hormones |
-
2010
- 2010-10-08 CN CN2010102989960A patent/CN101966254A/en not_active Withdrawn
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102247471A (en) * | 2010-10-08 | 2011-11-23 | 河南中医学院 | Application of rehmannia root water extract to preparing estrogen medicament |
| CN102247471B (en) * | 2010-10-08 | 2013-04-03 | 河南中医学院 | Application of rehmannia root water extract to preparing estrogen medicament |
| CN102579673A (en) * | 2012-03-15 | 2012-07-18 | 河南中医学院 | Application of fresh rehmannia root aqueous extract in preparation of estrogen medicines |
| CN110946921A (en) * | 2019-11-21 | 2020-04-03 | 张家港市中医医院 | Traditional Chinese medicine for relieving side effects of glucocorticoid medicaments |
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| Publication number | Publication date |
|---|---|
| CN102247471A (en) | 2011-11-23 |
| CN102247471B (en) | 2013-04-03 |
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Open date: 20110209 |