CN101962636B - Preparation process of high-activity liquid papain - Google Patents
Preparation process of high-activity liquid papain Download PDFInfo
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- CN101962636B CN101962636B CN 201010515885 CN201010515885A CN101962636B CN 101962636 B CN101962636 B CN 101962636B CN 201010515885 CN201010515885 CN 201010515885 CN 201010515885 A CN201010515885 A CN 201010515885A CN 101962636 B CN101962636 B CN 101962636B
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Abstract
The invention discloses a preparation process of high-activity liquid papain, comprising the steps of: mixing dry papayalatex and water together to obtain a papayalatex suspension, adding amylase, pectinase and saccharifying enzyme into the papayalatex suspension for enzymolysis; filtering the enzymolysis solution and taking filtrate; and carrying out membrane filtration of the filtrate for sterilization to obtain the high-activity liquid papain. The liquid papain prepared by the process has the enzyme activity not lower than 500,000unit/ml and the bacterial count not higher than 10,000/ml. In the process of the invention, production cycle is shorter, the whole production process can be completed within five days, and thus the production efficiency is greatly improved; all process parameters are easy to control, and the used equipment are easy to maintain; the extraction rate is close to 100%, and thus raw material waste is greatly reduced; and moreover, the process has the characteristics of low energy consumption, energy saving and environmental protection, and the comprehensive production cost of the process is reduced by more than 30% compared with the natural standing and digestion in the traditional process, therefore, the process is a green and environmental-friendly extraction process.
Description
Technical field
The present invention relates to a kind of preparation technology of liquid enzymes, particularly a kind of preparation technology of high vigor liquid papoid.
Background technology
Papoid is widely used in industries such as food, makeup, feed, leather processing, and is of many uses.At present the whole world year produces a papoid and reaches more than 1000 tons, and the annual demand of the U.S. is wherein most of by import just about 300 tons.The present papoid market demand of China is 300 tons, but the papoid YO is merely about 100 tons, and is mostly raw product, i.e. the dried product of pawpaw slurry.In recent years, all speed increments about with 5% in demand every year of papoid in the world.Market is huge to the demand of papoid.
Papoid is a kind of plant protease, mainly by from pawpaw, extracting.Common papoid has two kinds of solid and liquid.Though the solid enzyme helps prolonged preservation, in production operation, exist the inhomogeneous dust of technological fluctuation, enzyme that causes of interpolation of enzyme to be prone to cause problems such as allergy, during the historical facts or anecdotes border produces, use liquid enzymes more convenient.
At present, the production technique of China's liquid papoid mainly contains following two kinds: 1. leave standstill lixiviate naturally, extraction yield generally only has about 70%, and the commodity vigor is usually less than 100,000 units per ml, and production efficiency is low and the cycle is long, generally reaches 15 days; 2. dried papaya latex is ground to form pulpous state, impurity does not separate, and the commodity vigor is no more than 200,000 units per ml.Technology product vigor 1. is low, production efficiency is low and the cycle is long; Though the technology production cycle 2. is short, impurity is many, and sanitary index is also poor.Because the poor quality of papoid product causes the added value of these farm crop that are easy to cultivate of pawpaw low, has also influenced melon grower's income.
Therefore seek that a kind of product vigor is high, all high preferably vigor liquid papoid of sanitary index and economy aspect extraction production technology is extremely important.
Summary of the invention
The object of the present invention is to provide a kind of simply, the preparation technology of high vigor liquid papoid efficiently.
The technical scheme that the present invention taked is:
The preparation technology of high vigor liquid papoid may further comprise the steps:
1) dried papaya latex is mixed with water, obtain the papaya latex suspension liquid, add glycase, polygalacturonase, saccharifying enzyme, enzymolysis at the papaya latex suspension liquid;
2) enzymolysis solution is filtered, get filtrating;
3) filtrating is carried out the membrane filtration degerming, obtain high vigor liquid papoid.
Preferably, every 100g papaya latex (dry weight), the glycase of adding 1000～3000U.
Preferably, every 100g papaya latex (dry weight), the polygalacturonase of adding 1000～20000U.
Preferably, every 100g papaya latex (dry weight), the saccharifying enzyme of adding 5000～10000U.
Preferably, papaya latex (dry weight) is 10～100:100 with the mass ratio of water.
The temperature of enzymolysis is 21～57 ℃.
The liquid papoid of prepared of the present invention, enzyme work is not less than 500,000 units/ml, and bacterial count is not higher than 10000/ml.
Technology of the present invention, with short production cycle, can accomplish whole process of production within 5 days, production efficiency improves greatly.Each processing parameter is easy to control, and the equipment of use also for ease of maintenaince.
Technology of the present invention, extraction yield can significantly reduce the waste of raw material near 100%.
Technology of the present invention, energy consumption is low, energy-conserving and environment-protective, comprehensive production cost is conventional to leave standstill the lixiviate reduction more than 30% naturally, is the extraction process of a kind of green, environmental protection.
Embodiment
The preparation technology of high vigor liquid papoid may further comprise the steps:
Dried papaya latex is mixed with water, obtain the papaya latex suspension liquid, add glycase, polygalacturonase, saccharifying enzyme, enzymolysis at the papaya latex suspension liquid;
Enzymolysis solution is filtered, get filtrating;
Filtrating is carried out the membrane filtration degerming, obtain high vigor liquid papoid.
Preferably, every 100g papaya latex (dry weight), the glycase of adding 1000～3000U.
Preferably, every 100g papaya latex (dry weight), the polygalacturonase of adding 1000～20000U.
Preferably, every 100g papaya latex (dry weight), the saccharifying enzyme of adding 5000～10000U.
Preferably, papaya latex (dry weight) is 10～100:100 with the mass ratio of water.
The temperature of enzymolysis is 21～57 ℃.
Preferably, also be added with the sequestrant of papaya latex (dry weight) quality 0.1～1.2% in the papaya latex suspension liquid.Preferably, sequestrant is EDTA-Na
2
Below in conjunction with embodiment, further specify the present invention.
Employed umber in following examples if no special instructions, all refers to mass parts.
Embodiment 1
10 parts of dried papaya latexs are mixed with 100 parts of water, obtain the papaya latex suspension liquid,
1) ratio in the dried papaya latex adding of every 100g 1000U glycase, 6000U polygalacturonase, 6000U saccharifying enzyme adds glycase, polygalacturonase, saccharifying enzyme, 30 ℃ of enzymolysis;
2) enzymolysis solution is passed through the eardrum squeeze and filter, get filtrating;
3) heat sterilization of will filtrating obtains high vigor liquid papoid.
The whole production cycle is 4 days 5 hours, and the yield of papoid is 99.8%, and enzyme work is 600,000 units/ml.
Embodiment 2
1) 50 parts of dried papaya latexs are mixed with 100 parts of water, obtain the papaya latex suspension liquid,
2) ratio in the dried papaya latex adding of every 100g 1800U glycase, 10000U polygalacturonase, 5000U saccharifying enzyme adds glycase, polygalacturonase, saccharifying enzyme, 37 ℃ of enzymolysis;
3) enzymolysis solution is passed through the eardrum squeeze and filter, get filtrating;
4) heat sterilization of will filtrating obtains high vigor liquid papoid.
The whole production cycle is 4 days, and the yield of papoid is 99.5%, and enzyme work is 700,000 units/ml.
Embodiment 3
1) 30 parts of dried papaya latexs are mixed with 100 parts of water, obtain the papaya latex suspension liquid,
2) ratio in the dried papaya latex adding of every 100g 1300U glycase, 1000U polygalacturonase, 7000U saccharifying enzyme adds glycase, polygalacturonase, saccharifying enzyme, 57 ℃ of enzymolysis;
3) enzymolysis solution is passed through the eardrum squeeze and filter, get filtrating;
4) heat sterilization of will filtrating obtains high vigor liquid papoid.
The whole production cycle is 3 days 15 hours, and the yield of papoid is 97.2%, and enzyme work is 500,000 units/ml.
Embodiment 4
1) 80 parts of dried papaya latexs are mixed with 100 parts of water, obtain the papaya latex suspension liquid,
2) ratio in the dried papaya latex adding of every 100g 2500U glycase, 20000U polygalacturonase, 10000U saccharifying enzyme adds glycase, polygalacturonase, saccharifying enzyme, 42 ℃ of enzymolysis;
3) enzymolysis solution is passed through the eardrum squeeze and filter, get filtrating;
4) heat sterilization of will filtrating obtains high vigor liquid papoid.
The whole production cycle is 4 days 14 hours, and the yield of papoid is 99.0%, and enzyme work is 670,000 units/ml.
Embodiment 5
1) 100 parts of dried papaya latexs are mixed with 100 parts of water, obtain the papaya latex suspension liquid,
2) ratio in the dried papaya latex adding of every 100g 3000U glycase, 15000U polygalacturonase, 8500U saccharifying enzyme adds glycase, polygalacturonase, saccharifying enzyme, 21 ℃ of enzymolysis;
3) enzymolysis solution is passed through the eardrum squeeze and filter, get filtrating;
4) heat sterilization of will filtrating obtains high vigor liquid papoid.
The whole production cycle is 4 days 20 hours, and the yield of papoid is 98.5%, and enzyme work is 680,000 units/ml.
The present invention can discharge papoid through choosing suitable enzyme and appropriate vol apace from papaya latex, effectively shortened whole process flow, has improved production efficiency.
Though also can extract under the high temperature, the Controllable Temperature of whole production technology is built in below 40 ℃, and the enzyme work that can keep papoid like this is in higher level.Simultaneously, filtration, ultrafiltration all are pure physical operationss, and treatment capacity is big, and energy consumption is low, can further improve production efficiency, reduces production costs.
The liquid papoid of prepared of the present invention, enzyme work is not less than 500,000 units/ml, and bacterial count is not higher than 10000/ml, is a kind of product of excellent performance.This implementation of processes also will further improve the added value of pawpaw, help improving melon grower's income.
Technology of the present invention, with short production cycle, can accomplish whole process of production within 5 days, production efficiency improves greatly.Each processing parameter is easy to control, and the equipment of use also for ease of maintenaince.
Technology of the present invention, extraction yield can significantly reduce the waste of raw material near 100%.
Technology of the present invention, energy consumption is low, energy-conserving and environment-protective, comprehensive production cost is conventional to leave standstill the lixiviate reduction more than 30% naturally, is the extraction process of a kind of green, environmental protection.
Claims (3)
1. the preparation technology of high vigor liquid papoid may further comprise the steps:
1) dried papaya latex is mixed with water, obtain the papaya latex suspension liquid, add glycase, polygalacturonase, saccharifying enzyme, enzymolysis at the papaya latex suspension liquid;
2) enzymolysis solution is filtered, get filtrating;
3) filtrating is carried out the membrane filtration degerming, obtain high vigor liquid papoid;
Wherein, every 100g papaya latex (dry weight) adds the glycase of 1000～3000U, the polygalacturonase of 1000～20000U, the saccharifying enzyme of 5000～10000U; The temperature of enzymolysis is 21～57 ℃; Papaya latex (dry weight) is (10～100) with the mass ratio of water: 100.
2. the preparation technology of high vigor liquid papoid according to claim 1 is characterized in that: the sequestrant that also is added with papaya latex (dry weight) quality 0.1～1.2% in the papaya latex suspension liquid.
3. the preparation technology of high vigor liquid papoid according to claim 2 is characterized in that: sequestrant is EDTA-Na
2
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|---|---|---|---|
| CN 201010515885 CN101962636B (en) | 2010-10-22 | 2010-10-22 | Preparation process of high-activity liquid papain |
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| CN 201010515885 CN101962636B (en) | 2010-10-22 | 2010-10-22 | Preparation process of high-activity liquid papain |
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| CN101962636B true CN101962636B (en) | 2012-12-05 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103602649B (en) * | 2013-11-07 | 2015-09-30 | 陆祥 | A kind of method of purification of papoid |
| CN109321554B (en) * | 2018-11-30 | 2021-11-09 | 广西南宁天绿生物制品有限公司 | Production method of special papain for beer |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1224060A (en) * | 1999-01-07 | 1999-07-28 | 王元志 | Method for large scale production of corn superoxide dismutase (SOD) complex enzyme |
| CN1597946A (en) * | 2004-08-09 | 2005-03-23 | 王美岭 | Process for preparing papain |
| WO2007076776A1 (en) * | 2005-12-14 | 2007-07-12 | Norbert Steiner | Method and device for vacuum drying papaya latex |
| CN101570748A (en) * | 2008-05-04 | 2009-11-04 | 徐闻县美仑生物制品有限公司 | Method for refining papain |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3126324B2 (en) * | 1997-06-04 | 2001-01-22 | 光雄 平良 | Extraction and culture method of papain enzyme |
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- 2010-10-22 CN CN 201010515885 patent/CN101962636B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1224060A (en) * | 1999-01-07 | 1999-07-28 | 王元志 | Method for large scale production of corn superoxide dismutase (SOD) complex enzyme |
| CN1597946A (en) * | 2004-08-09 | 2005-03-23 | 王美岭 | Process for preparing papain |
| WO2007076776A1 (en) * | 2005-12-14 | 2007-07-12 | Norbert Steiner | Method and device for vacuum drying papaya latex |
| CN101570748A (en) * | 2008-05-04 | 2009-11-04 | 徐闻县美仑生物制品有限公司 | Method for refining papain |
Non-Patent Citations (1)
| Title |
|---|
| JP特开平10-327856A 1998.12.15 |
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