CN101953246B - Indoor germination percentage inspection method of Chinese iris seed rooms - Google Patents
Indoor germination percentage inspection method of Chinese iris seed rooms Download PDFInfo
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- CN101953246B CN101953246B CN 201010532213 CN201010532213A CN101953246B CN 101953246 B CN101953246 B CN 101953246B CN 201010532213 CN201010532213 CN 201010532213 CN 201010532213 A CN201010532213 A CN 201010532213A CN 101953246 B CN101953246 B CN 101953246B
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Abstract
The invention relates to an indoor germination percentage inspection method of Chinese iris seeds, belonging to a seed inspection method. The method is realized by the following steps of: cleaning and sterilizing a culture dish with the diameter of 12 cm, and laying five layers of sterile filter paper with the diameter of 11.5 cm at the bottom of the culture dish; fully mixing Chinese iris seeds in a 0.2% thiophanate methyl solution in the mass ratio of 1:(6-9) for sterilizing for 3 min, and washing with sterile water 5-8 times, 2-3 min each time; fully wetting the filter paper in the culture dish by using 0.2% thiophanate methyl solution, draining excessive medicinal liquid after the filter paper absorbs enough medicinal liquid, uniformly arranging 50 seeds on the culture dish with radicle extending parts turning upward, covering the culture dish, coating with two layers of aluminium-foil paper, and placing in an illumination incubator; illuminating 1250 LX at 30 DEG C in daytime for6-8 h, and keeping 20 DEG C in darkness at night for 16-18 h; and counting the germination percentage of the seeds on the 28th day, and finishing the germination experiment. The indoor germination percentage inspection method of Chinese iris seeds provides suitable germination conditions according to the characteristics of the Chinese iris seeds, so that the germinating ability of the seeds is ensured to be exerted. The invention has the advantages of simple operation, high accuracy more than 98% and short time (finishing in 28 days).
Description
Technical field
The invention belongs to the method for seed testing, particularly relate to the indoor germination rate method of inspection of a kind of chinensis seed.
Background technology
Chinese small iris, another name Ma Lian, flagger is an Iridaceae Jris herbaceos perennial.Be distributed widely in northern China and west area.The Chinese small iris strong stress resistance, especially salt tolerant alkali is the constructive species of saline meadow; Because its well developed root system, drought-resistant ability is strong, and blade is turned green early, the green phase is long, pattern is simple and elegant; Make its urban afforestation that is very suitable for northern China and west area, water and soil conservation and alkaline land improving, urban afforestation can be widely used in urban afforestation, the water and soil seed quality check in open greenery patches, northern China and west area and find, the chinensis seed difficulty of germinateing in the laboratory; The result difference that conventional germination test detects is big, and repetitive rate is low, and detection time is long; Can not meet the demands, and the laboratory does not have corresponding inspection technology yet, bring a lot of obstacles for the application of Chinese small iris.Therefore, press for a kind of indoor method of inspection that can accurately detect the Chinese small iris germinating capacity.
Summary of the invention
The present invention is intended to overcome the deficiency of prior art, provides a kind of chinensis seed the indoor germination rate method of inspection.
The applicant discovers that chinensis seed is sprouted has the characteristics that are different from general seed: mainly be requirement to light, and very easily mouldy in addition.Conventional seed testing is divided into light seed and dark seed with the seed germination condition; Perhaps give illumination or give dark; And chinensis seed is to need scattered light and avoid high light to the requirement of light; The culture dish that must will set seed is tight with the aluminium-foil paper parcel, is placed in the incubator of illumination condition, and culture effect is best.
The suitable sprouting temperature of chinensis seed should be 20～30 ℃ of alternating temperatures, cooperates 30 ℃ of illumination daytime 6～8 hours, night dark 20 ℃ 16～18 hours.Chinensis seed exposes directly that the seed germination ability is suppressed under illumination condition, but the seed germination is also bad under dark condition fully.Have the advantages that to avoid high light happiness scattered light, culture dish needs with the aluminium-foil paper parcel tight, and it is best to be placed in the incubator of illumination condition culture effect.
Chinese small iris has abundant kind suitcase to wrap up in, and endosperm is the main component that constitutes seed in addition, and seed sprouting speed is slower; Under no soil condition; Seed is very easily mouldy, and handling mouldy problem well is to guarantee the successful key of seed sprouting, therefore; Seed is conscientiously sterilized, and is the mouldy key of control with the 0.2% thiophanate methyl aqueous solution as culture fluid.
In view of the above, the indoor germination rate method of inspection of chinensis seed of the present invention, realize through following method:
The preparation of a, germinating bed: diameter is cleaned for the 12cm culture dish and sterilizes, be laid on the culture dish bottom with five layers of aseptic filter paper of diameter 11.5cm then;
B, seed sterilization: chinensis seed is pressed mass ratio 1:6～9 in 0.2% thiophanate methyl solution, fully mix sterilization 3 minutes, use sterile water wash 5～8 times then, each 2～3 minutes;
C, seed germination and check: culture dish is fully wetting with filter paper with 0.2% thiophanate methyl solution, suction soup after, drop removes unnecessary soup; Put into 50 seeds, the radicle extending area up, evenly is arranged on the culture dish, culture dish is covered and wrap two-layer aluminium-foil paper, put into illumination box; 30 ℃ of 1250LX illumination on daytime 6～8 hours; Night dark 20 ℃ 16～18 hours, can add up percentage of seedgermination on the 28th day, finish the experiment of germinateing.
If in germination process, can not keep gnotobasis, need to replace distilled water that seed is provided moisture and continues sterilization with 0.2% thiophanate methyl.
Adopting above-mentioned illumination and sterilization treatment, guarantee moisture in conjunction with five metafiltration paper, is the key that guarantees that Chinese small iris germinates, and also is characteristics of the present invention.
The indoor germination rate method of inspection of chinensis seed of the present invention according to the characteristic of chinensis seed, provides suitable sprouting condition; Thereby guarantee that germination capacity of seeds obtains performance; Have simple to operate, accuracy rate is high, reaches more than 98% the advantage of time short (28d completion).
Embodiment
The indoor germination rate method of inspection of chinensis seed of the present invention is that earthing the following step is realized:
Germinating bed is prepared.Culture dish adopts diameter 12cm; Thoroughly clean and sterilize, be laid on the culture dish bottom with five layers of diameter 11.5cm aseptic filter papers then, seed sprouting bed five metafiltration paper; Therefore the more and special light treatment of need because of duration of germination seed water requirement needs with five metafiltration paper;
The seed sterilization.0.2% thiophanate methyl solution is pressed mass ratio 1:8, fully mixes sterilization 3 minutes, uses sterile water wash then 7 times, each 2-3 minute.If can not guarantee gnotobasis in the germination process, need to replace distilled water that seed is provided moisture and continues sterilization with 0.2% thiophanate methyl;
Each culture dish is fully wetting with filter paper with 0.2% thiophanate methyl solution, suction soup after, drop removes unnecessary soup; Put into 50 seeds, with the radicle extending area up, evenly arrange; After setting seed culture dish is covered, wrap aluminium-foil paper, put into illumination box; Condition of culture was set to 30 ℃ of 1250LX illumination on daytime 8 hours, night dark 20 ℃ 16 hours, checked moisture and germination in per seven days; Record is in time removed and carried out to the discovery mildew seed, can add up the seed sprouting situation on the 28th day, finishes the experiment of germinateing.For being improves to detect accuracy rate, each batch seed carries out above-mentioned duplicate test at least four times.
The vitality and the relevant factor of germinateing in the face of chinensis seed experimentizes down, and the result is following:
1, the viability test of chinensis seed
Seed vigor is potential ability of seed sprouting and the vitality that embryo is had.Assay method carries out with reference to " blodynamic biochemistry (tetrazolium) mensuration " method in " grass seed inspection procedure ".Random number is got 100 seeds; 4 repetitions; Remove seed dormancy (reference literature: Sun Y. C. (Sun Yuechun), Zhang Y. J., Wang K. and Qiu X. J. NaOH scarification and stratification improve germination of
Iris lacteaVar.
ChinensisSeed. Hortscience 2006; 41 (3): 773-774), with 20 ℃ of following distilled water 24h that prewets, then near embryo with the seed rip cutting; Embryo is exposed; Tetrazolium solution with 1% 24h that under 30 ℃ of dark conditions, dyes observes evaluation then under three-dimensional anatomical lens, with embryo dye fully for having blodynamic seed.Get three differences gather the year seed detect respectively.The result:
| The seed of gathering in 2006 | The seed of gathering in 2007 | 2009 (then) seed of gathering | |
| Vitality (%) | 62.35% | 65.23% | 87.75% |
2, illumination is to the influence of iris lactea seed germination
Adopt diameter 12cm culture dish, thoroughly clean and sterilize, be laid on culture dish bottom (five metafiltration paper can keep than juicy, and assurance need not be opened culture dish and kept the skin wet more than seven days) with five layers of diameter 11.5cm aseptic filter papers then.Seed and 0.2% thiophanate methyl solution are pressed mass ratio 1:8, fully mix sterilization 3 minutes.Each culture dish is fully wetting with filter paper with 0.2% thiophanate methyl solution, suction soup after, drop goes unnecessary soup to get final product; Put into 50 seeds, with the radicle extending area up, evenly separately; After setting seed culture dish is covered, put into respectively a continuous darkness, continuous 1 250 luxs of b (Lx) illumination, c with the layer of aluminum foil paper with culture dish Bao Yan, when temperature being set by day being 30 ℃; Give 1 250 luxs (Lx) illumination 8h; Dark is set, 20 ℃ of temperature, 16h night.Each batch seed sprouting situation is following:
Seed germination is regulated by light, confirms its position in soil through light intensity and optical wavelength.The Chinese small iris suitable growth is in soil environment barren, slight salinization of soil, and seed germination speed is slower, and embryo is less, but nutrition is stored abundanter in the endosperm; Therefore in the process that conforms for a long time, selected in soil certain depth to sprout gradually, confirmed the position in soil, the hydrothermal condition more stable than soil surface can be provided in the soil through light; Too dark when what in soil, bury, seed does not have enough energy to grow ground, and seed is overhead too near; Humidity changes too greatly, can not satisfy the moisture in the germination process, therefore; Too dark or too shallow unsuitable sprouting the in soil, and seed is confirmed its position in soil, light plays an important role.Find out that from top data illumination suppresses chinensis seed sprouts, but dark also has certain inhibition fully, tight with aluminium aluminium foil paper bag, the imitation seed is in the situation of several centimetres of subsurfaces, and the germination effect is best, and rate of accuracy reached is more than 98%.
3, the relation of sterilization in the germination process and germination rate (distilled water, sterile water and 0.2% thiophanate methyl solution are relatively): after 2009 years batches of seed sterilizations; Adopting distilled water, sterile water, 0.2% thiophanate methyl solution respectively is that seed provides moisture in the culture dish; Statistics is cultivated 28d germination rate, mould variability.
| ? | Distilled water | Sterile water | 0.2% thiophanate methyl solution |
| Germination rate | 12.35% | 16.64% | 86.75% |
| Accuracy rate | 14.07% | 18.96% | 98.86% |
| Mould variability | 73.4% | 65.8% | 0.56% |
In the test, the reason of the repeatedly failure of germinateing is a mould contamination, rotten, the mouldy test failure that causes; (the fourth pre-treating method that promotes chinensis seed to sprout such as spur oneself on is inquired into (bulletin) for list of references: Wang Yongchun, Sun Qun; The meadow journal, 2009,17 (2): 264-266) similar experience is arranged; Through the expert of consulting corresponding plants seed disease, after pass through test of many times again, confirm that at last 0.2% thiophanate methyl solution replaces water as culture fluid; Can guarantee seed not by or few (being less than 1%) by mycotic infection, thereby guarantee to germinate just often go rate of accuracy reached 98.86%.
4, seed putting position and germination rate: after the sterilization of 2009 years batches of seeds, adopt respectively that the radicle extending area (is attached on the filter paper), put the radicle extending area down up and at random, statistics is cultivated the 28d germination rate.
| ? | The radicle extending area down | The radicle extending area up | Put at random |
| Germination rate | 20.54% | 87.22% | 58.52% |
| Accuracy rate (comparing) with vitality | 23.41% | 99.40% | 66.69% |
Find in the test,, when running into filter paper, often will stop the further elongation of radicle, influence the germination result, and when the radicle extended position upwards put, best germination result is arranged, rate of accuracy reached 99.40% when radicle stretches out.
Claims (1)
1. the indoor germination rate method of inspection of chinensis seed, realize through the following step:
The preparation of a, germinating bed: diameter is cleaned for the 12cm culture dish and sterilizes, be laid on the culture dish bottom with five layers of aseptic filter paper of diameter 11.5cm then;
B, seed sterilization: chinensis seed and 0.2% thiophanate methyl solution are pressed mass ratio 1:6～9, fully mix sterilization 3 minutes, use sterile water wash 5～8 times then, each 2～3 minutes;
C, seed germination and check: culture dish is fully wetting with filter paper with 0.2% thiophanate methyl solution; After suctioning soup, drop removes unnecessary soup, puts into 50 seeds, the radicle extending area up, evenly is arranged on the culture dish; Culture dish is covered and wraps two-layer aluminium-foil paper; Put into illumination box, be that 30 ℃, intensity of illumination be the condition of 1250LX under in temperature daytime, continues 6～8 hours; Continue 16～18 hours night under temperature is 20 ℃, no optical condition; The 28th day statistics percentage of seedgermination finishes the experiment of germinateing; Wherein, with 0.2% thiophanate methyl seed is provided moisture and continues sterilization in the seed sprouting.
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| CN 201010532213 CN101953246B (en) | 2010-11-05 | 2010-11-05 | Indoor germination percentage inspection method of Chinese iris seed rooms |
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| CN 201010532213 CN101953246B (en) | 2010-11-05 | 2010-11-05 | Indoor germination percentage inspection method of Chinese iris seed rooms |
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| CN101953246B true CN101953246B (en) | 2012-05-09 |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099422A (en) * | 2007-07-25 | 2008-01-09 | 毕慧滨 | Method for improving iris lactea seed germination rate |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099422A (en) * | 2007-07-25 | 2008-01-09 | 毕慧滨 | Method for improving iris lactea seed germination rate |
Non-Patent Citations (3)
| Title |
|---|
| 姚柳焓.不同处理方法对马蔺种子萌发的影响.《安徽农业科学》.2008,第36卷(第26期),第11197、11199页. * |
| 孙跃春.马蔺种子休眠解除技术与适宜萌发条件研究.《中国优秀博硕士学位论文全文数据库(硕士)农业科技辑》.2004,(第3期),第D048-124页. * |
| 王永春等.促进马蔺种子萌发的前处理方法探讨（简报）.《草地科学》.2009,第17卷(第2期),第264-266页. * |
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