CN103355025A - Method for rapidly identifying resistance to verticillium wilt of eggplant - Google Patents
Method for rapidly identifying resistance to verticillium wilt of eggplant Download PDFInfo
- Publication number
- CN103355025A CN103355025A CN2013102646752A CN201310264675A CN103355025A CN 103355025 A CN103355025 A CN 103355025A CN 2013102646752 A CN2013102646752 A CN 2013102646752A CN 201310264675 A CN201310264675 A CN 201310264675A CN 103355025 A CN103355025 A CN 103355025A
- Authority
- CN
- China
- Prior art keywords
- eggplant
- verticillium wilt
- seedling
- root
- identification method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a method for rapidly identifying resistance to verticillium wilt of eggplant. The method comprises the following steps of: accelerating germination and sowing of eggplant seeds, seedling culturing, preparation of a verticillium wilt spore suspension, treatment of pathogenic bacteria infection and incidence investigation. According to the invention, eggplant seedlings are infected with verticillium wilt in a manner of soaking and infecting seedling roots by a bacteria solution, and the eggplant seedlings are infected after being uniformly cultured in a substrate until achieving a state of one seedling with two leaves, so that the growth vigor and size of the eggplant seedlings are easy to control, and the consistency and reliability of an identification result are ensured. By adopting the appropriate verticillium wilt substrate and adjusting the concentration of the verticillium wilt suspension, the method has the advantages of good infection effect, high survival rate of the infected seedlings, uniform inoculation and rapid incidence. According to the invention, only different plants with consistent growth vigor need to be soaked in the bacteria solution after root cutting, so that the operation is simple and convenient, the seedling culture time is short, and the method is particularly suitable for identification of resistance to verticillium wilt of eggplant breeding materials in batches.
Description
Technical field
The invention belongs to the resistance plant detection field, be specifically related to a kind of rapid identification method of eggplant verticillium wilt resistance.
Background technology
Eggplant verticillium wilt (Verticillium wilt) is a kind of worldwide soil-borne disease, general time incidence of disease 15%-20%, and the serious time incidence of disease reaches more than 70%, cause the significant underproduction of eggplant, even have no harvest, have the title (Zeng etc., 2009) of eggplant " cancer ".In recent years, along with the expansion of eggplant cultivated area, the continuous cropping phenomenon is day by day serious, and eggplant verticillium wilt is increased the weight of year by year.This disease is the complete stool disease, and illness spreads to complete stool from bottom to top or from one side of something, and classical symptom comprises withered type, macula lutea type, yellow mottle type and dwarf-type 4 types of (Dong Jin target, 2001; Dong Guoju and He Yunchun, 2004).The morbidity thermophilic is 20-30 ℃, and humidity is larger under the thermophilic, falls ill more serious.The sick leaf of their early stage is wilted when fine high temperature, sooner or later can recover, and the later stage, sick leaf was brown by xanthochromia, final withered coming off, and complete stool is withered when serious.Studies show that, verticillium dahliae is main verticillium wilt pathogenic bacteria (Lu Jiayun, 1997 that harm China vegetables and crops produce; Zhu Jianlan and Chang Yongyi, 2004), the host is very extensive, can infect 38 sections, 660 various crop such as Solanaceae, Cruciferae, pulse family, Curcurbitaceae, the rose family, composite family, Labiatae, and host range is still enlarging (Pegg, 1989 year by year; Klosterman etc., 2009).This pathogen is by obstruction conduit, secretion lipopolysaccharides albumen composition and utilize the mode such as some extracellular enzyme degradation of cell wall, causes verticillium wilt.
Cultivating disease-resistant variety is the fundamental means of control eggplant verticillium wilt.The acquisition of resistance resource is significant for breeding for disease resistance, and is how objective, to estimate eggplant strain resistance to verticillium wilt ground power rapidly be the most basic, most important link in the eggplant resisting verticillium breeding process.For a long time, the method that the breeder adopts the pathogeny garden directly to induce morbidity or Seedling Stage soil to connect bacterium more is identified the resistance to verticillium wilt of germ plasm resource, the former depends critically upon the natural occurrence condition, if environmental condition is not suitable for morbidity or pathogeny garden germ skewness, easily cause the resistance erroneous judgement, and the latter needs soil to connect bacterium, also easily exists to connect the inhomogeneous situation of bacterium, causes qualification result inaccurate.
If find the method for Rapid identification eggplant verticillium wilt resistance, will greatly accelerate the process of eggplant resisting verticillium breeding undoubtedly.Therefore, all need a kind ofly to inoculate that rear eggplant survival rate is high in scientific research and the practice, morbidity rapidly, short, the simple and convenient management of seedling raise period, be fit to the rapid identification method of the eggplant verticillium wilt resistance that breeding material in enormous quantities identifies.
Summary of the invention
The object of the present invention is to provide a kind of rapid identification method of eggplant verticillium wilt resistance, the method comprises the steps such as the preparation, pathogen infection processing, incidence survey of eggplant seed vernalization, sowing and seedling culture, verticillium wilt spore suspension.After the method inoculation high, the morbidity of eggplant survival rate rapidly, short, the simple and convenient management of seedling raise period, be fit to breeding material in enormous quantities and identify.
The objective of the invention is to be achieved through the following technical solutions:
A kind of rapid identification method of eggplant verticillium wilt resistance, the method may further comprise the steps:
A, eggplant seed vernalization:
Select Eggplant Germplasm Resources as identifying object;
Select the seed of the full seed of Eggplant Germplasm Resources, carry out hoting water treatment of seeds in 50 ℃, 30 minutes, seed is placed in the culture dish of completing blotting paper again, last 30 ℃ of constant temperature are processed, until the seed that obtains sprouting;
B, sowing and seedling culture:
The planting seed sprouted in culture medium for seedling, is cultivated, obtained two leaves seedling wholeheartedly;
The preparation of C, verticillium wilt spore suspension:
A. select field typical case diseased plant, the position of eggplant plant pathogen possibility comparatively dense is cut apart;
B. with above-mentioned pathogen may comparatively dense the position disinfection, position after processing is sterilized;
C. dissect at the position after will disinfecting, and obtains the marrow tissue, and will state again the marrow tissue and be cut into the 0.5cm segment, and in the access PDA plating medium;
D. after growing white colony around the plants on surface tissue of postvaccinal PDA plating medium, this white colony changed in the PDA slant medium save backup;
E. from the PDA slant medium that changes white colony over to, get the Huang bacterium that withers, change in the PDA plating medium, carry out 27 ℃ of constant temperature culture 2-3 weeks, obtain the cultured Huang bacterium bacterium piece that withers;
F. the bacterium bacterium piece that cultured Huang withered vibrates in sterile water, filters, and utilizes blood counting chamber to adjust spore concentration, obtains the Huang bacterium spore suspension that withers;
D, pathogen infection are processed:
A. two leaves seedling is wholeheartedly extracted from described culture medium for seedling, carried out cleaning treatment, hinder again root and process, obtain hindering the seedling behind the root;
The seedling that b. will hinder behind the root is infected processing, obtains infecting seedling;
E, incidence survey:
Respectively at 5 days, 10 days, 15 days, 20 days that infect after the processing, investigate the described onset state that infects seedling, according to eggplant verticillium wilt severity Scaling standard 0,1,2,3,4 totally 5 the morbidity grades, according to this morbidity rating calculation disease index, judge at last the eggplant verticillium wilt resistance classification of described Eggplant Germplasm Resources according to this disease index again.
Above-mentioned eggplant verticillium wilt severity Scaling standard is:
0 grade: without diseased plant; 1 grade: complete stool yellow wilting blade is less than 1/4; 2 grades: complete stool yellow wilting blade is more than or equal to 1/4 and less than 1/2; 3 grades: complete stool yellow wilting blade accounts for more than or equal to 1/2 and less than 3/4; 4 grades: complete stool yellow wilting blade reaches withered to all wilting more than 3/4.
Disease index (DI) account form is:
Disease index=∑ (progression * strain number)/(highest number * total strain number) * 100.
Above-mentioned eggplant verticillium wilt resistance grade scale is as follows:
Disease-resistant (R): DI≤15; In anti-(MR): 15＜DI≤30; Anti-disease (T): 30＜DI≤50; Middle sense (MS): 50＜DI≤70; Susceptible (S): DI＞70.
Above-mentioned Eggplant Germplasm Resources comprises distant eggplant No. 6, the green eggplant in Xi'an, distant eggplant No. 3, distant eggplant No. 5, Tianjin soon circle and Solanum Torvum.
The position of above-mentioned pathogen possibility comparatively dense is stem foot, cane, petiole.
The preparation method of above-mentioned PDA plating medium is: peeling potato 200 g, be cut into the approximately fritter of 1.5 cm of the length of side, add 1000mL water boil 30 min, with 4 layers of filtered through gauze, in filtrate, add glucose 20 g and agarose 15 g, supply again water to 1000 mL after the heating for dissolving; The pH nature, 121 ℃, 30 min sterilization; Be down flat plate at superclean bench afterwards, obtain the PDA plating medium.
The above-mentioned Huang bacterium spore suspension spore concentration that withers is 0.8-1.5 * 10
7Individual/ml, be preferably
1 * 10 7Individual/ml.
The above-mentioned root of hindering is treated to: wiping out length is 1.5-2.5cm, is preferably the main root tip of a root and the corresponding fibrous root of 2cm.
Above-mentionedly infecting processing, is that the eggplant seedling of will hinder behind the root is placed in the verticillium wilt spore suspension, blocks vessel port with aseptic gas permeable material; The seedling that will be positioned in the verticillium wilt spore suspension again is incubated at greenhouse by solar heat; Environmental temperature is 25-30 ℃ of daytime, 15-20 ℃ of night.
The present invention has following beneficial effect compared to existing technology:
1, make eggplant seedling infect verticillium wilt pathogen owing to adopting bacterium liquid to soak the mode that infects seedlings root, eggplant seedling to hinder the root degree consistent, guaranteed uniformity and the reliability of qualification result.
2, owing to eggplant seedling is unified in first is cultured to two leaves in the seedling medium and wholeheartedly infects again, the growing way of eggplant seedling, size are easy to control, have guaranteed uniformity and the reliability of qualification result.
3, since taked suitable verticillium wilt spore medium, adjusted the concentration of verticillium wilt spore suspension, therefore infect effect better, infect that the seedling survival rate is high, to connect bacterium even, morbidity is rapidly.
4, the different plants that growing way is consistent is hindered to be immersed in the bacterium liquid behind the root and got final product, and is easy and simple to handle, seedling raise period is short, and the resistance to verticillium wilt that especially is fit to large batch of eggplant breeding material is identified.
Description of drawings
Fig. 1 is the micro-structure diagram of the bacterium colony of PDA slant medium described in the step C-d of embodiment.
Fig. 2 infects in the step e of embodiment to process rear 15 days, and the Disease Resistance Identification of the green eggplant of Solanum Torvum and Xi'an is figure as a result.A is not for connecing the contrast of bacterium clear water, and B processes for connecing bacterium.
Embodiment
The rapid identification method of eggplant verticillium wilt resistance may further comprise the steps:
A, eggplant seed vernalization:
Select dissimilar Eggplant Germplasm Resources as identifying object, comprise distant eggplant No. 6, the green eggplant in Xi'an, distant eggplant No. 3, distant eggplant No. 5, Tianjin soon circle and Solanum Torvum.
Select the seed of the full seed of described Eggplant Germplasm Resources, hot water treatment of seeds, temperature is 50 ℃, time is 30 minutes, and seed is fully absorbed water, and seed is placed on to carry out the constant temperature processing in the culture dish of completing blotting paper again, 30 ℃ of temperature, until seed germination, the seed that obtains sprouting;
B, sowing and seedling culture:
The planting seed sprouted in culture medium for seedling, is cultivated, obtained two leaves seedling wholeheartedly;
The preparation method of culture medium for seedling is: the peat composed of rotten mosses, vermiculite and chicken manure according to weight ratio 9:3:1 mixing, are carried out autoclaving 1.5h in 121 ℃ again, obtain culture medium for seedling;
The operation of sowing: be 1% disinfecting solution of potassium permanganate with instrument service property (quality) concentration with sowings such as seedling dishes; The seedling-cultivating tray (conventional 32 holes) of again culture medium for seedling being packed into waters permeable; Again the seed of sprouting is lain on the culture medium for seedling culture medium for seedling of cladding thickness 1cm in the above again, again covering with plastic film moisturizing;
Cultivation is that Routine Management is grown seedlings in greenhouse by solar heat, with after planting seed culture to two leaf wholeheartedly.
The preparation of C, verticillium wilt spore suspension:
A. separate according to Koch's Postulates and identify selection field typical case diseased plant, the eggplant plant is divided into the position of pathogen possibility comparatively dense with the cutter after the sterilization: stem foot, cane, petiole;
B. with the position disinfection of above-mentioned pathogen possibility comparatively dense, namely use the alcohol wipe surface of concentration 70%, use again aseptic water washing 3 times; Position after processing is sterilized;
C. the position after above-mentioned the disinfecting is dissected, the position after namely will disinfecting with the cutter after the sterilization is vertically cut open, notes not polluting its marrow;
Obtain the marrow tissue, again the marrow tissue is cut into length 0.5cm segment, and in the access PDA plating medium;
The preparation method of PDA plating medium: peeling potato 200 g, be cut into the approximately fritter of 1.5 cm of the length of side, add 1000mL water boil 30 min, with 4 layers of filtered through gauze, in filtrate, add glucose 20 g and agarose 15 g, supply again water to 1000 mL after the heating for dissolving; The pH nature, 121 ℃, 30 min sterilization; Be down flat plate at superclean bench afterwards, obtain the PDA plating medium.
D. after growing white colony around the plants on surface tissue of postvaccinal PDA plating medium, this white colony is carried out the microscopy (see figure 1), A is conidium, and B is the wheel branch structure of mycelia, and C is spore ball, and D is sclerote.Bacterium colony after microscopy confirmed changes in the PDA slant medium to be preserved;
The preparation of PDA slant medium:
Raw material, proportioning and sterilising conditions are poured medium in the 20mL test tube that tilts to put on superclean bench afterwards with the PDA plating medium, obtain the PDA slant medium.
E. from the PDA slant medium, get the Huang bacterium that withers, change in the PDA plating medium, in 27 ℃ of constant temperature culture 20 days, obtain the cultured Huang bacterium bacterium piece that withers;
F. under 27 ℃ of conditions, the cultured Huang bacterium bacterium piece that withers is placed the triangular flask that the fills sterile water 48h that vibrates, then filter through 4 layers of sterile gauze, add up spore count at microscopically with blood counting chamber, with sterile water spore is transferred to 1 * 10
7The spore suspension of individual/ml; The bacterium spore suspension that again the 100mL Huang withered is placed in the 250mL triangular flask for subsequent use;
D, pathogen infection are processed:
A. two leaves seedling is wholeheartedly taken out from cultivate seedling medium, carry out cleaning treatment, namely use the wholeheartedly culture medium for seedling of the root of seedling of distilled water flush away two leaves, with clean filter paper the moisture on the root is blotted again; Hinder again root and process, namely cut off the main root tip of a root and the corresponding fibrous root that length is 2cm with scissors; Obtain hindering the seedling behind the root;
The seedling that b. will hinder behind the root is placed in the verticillium wilt spore suspension, and the triangle bottleneck is blocked with sterile cotton; Be positioned over greenhouse by solar heat, environmental temperature daytime is 25-30 ℃, and be 15-20 ℃ night, obtains infecting seedling;
E, incidence survey:
Respectively at 5 days, 10 days, 15 days, 20 days that infect after the processing, investigate the described onset state that infects seedling, according to eggplant verticillium wilt severity Scaling standard 0,1,2,3,4 totally 5 the morbidity grades, according to this morbidity rating calculation disease index, judge again the eggplant verticillium wilt resistance classification of Eggplant Germplasm Resources according to this disease index again.
Eggplant verticillium wilt severity Scaling standard is:
0 grade: without diseased plant; 1 grade: complete stool yellow wilting blade is less than 1/4; 2 grades: complete stool yellow wilting blade is more than or equal to 1/4 and less than 1/2; 3 grades: complete stool yellow wilting blade accounts for more than or equal to 1/2 and less than 3/4; 4 grades: complete stool yellow wilting blade reaches withered to all wilting more than 3/4.
Disease index (DI) account form is:
Disease index=∑ (progression * strain number)/(highest number * total strain number) * 100.
The formula explanation:
Progression: morbidity grade, 0-4 level;
Strain number: the strain number that identical morbidity grade is corresponding;
Highest number: the highest morbidity grade that occurs in the state of an illness fact-finding process;
Total strain number: all eggplant plant numbers that carry out state of an illness investigation.
Eggplant verticillium wilt resistance grade scale is as follows:
Disease-resistant (R): DI≤15; In anti-(MR): 15＜DI≤30; Anti-disease (T): 30＜DI≤50; Middle sense (MS): 50＜DI≤70; Susceptible (S): DI＞70.
The result shows, infects and processes rear 5 days, and except Solanum Torvum, slight wilting appears in other examination materials; Infect and processed rear 10 days, Solanum Torvum does not still show wilting, and wilting all appears in other examination materials, and wherein slight yellow appears in the green eggplant partial blade in Xi'an; Infect and processed rear 15 days, Solanum Torvum does not show susceptible symptom, and wilting all appears in all the other examination materials, and with in various degree yellow, the different cultivars disease resistance presents obvious difference; Infect and processed rear 20 days, susceptible difference is similar with infecting rear 15 days.
Summary draws, and Solanum Torvum is disease-resistant variety, and the fast circle in the green eggplant in Xi'an and Tianjin is susceptible variety, and distant eggplant No. 6, distant eggplant No. 3, distant eggplant are disease tolerant variety No. 5.Fig. 2 infects the Disease Resistance Identification result who processes the rear 15 days green eggplants of Solanum Torvum and Xi'an.A is not for connecing the contrast of bacterium clear water, and B processes for connecing bacterium.
Claims (9)
1. the rapid identification method of an eggplant verticillium wilt resistance, it is characterized in that: the method may further comprise the steps:
A, eggplant seed vernalization:
Select Eggplant Germplasm Resources as identifying object;
Select the seed of the full seed of Eggplant Germplasm Resources, carry out hoting water treatment of seeds in 50 ℃, 30 minutes, seed is placed in the culture dish of completing blotting paper again, last 30 ℃ of constant temperature are processed, until the seed that obtains sprouting;
B, sowing and seedling culture:
The planting seed sprouted in culture medium for seedling, is cultivated, obtained two leaves seedling wholeheartedly;
The preparation of C, verticillium wilt spore suspension:
A. select field typical case diseased plant, the position of eggplant plant pathogen possibility comparatively dense is cut apart;
B. with above-mentioned pathogen may comparatively dense the position disinfection, position after processing is sterilized;
C. dissect at the position after will disinfecting, and obtains the marrow tissue, and will state again the marrow tissue and be cut into the 0.5cm segment, and in the access PDA plating medium;
D. after growing white colony around the plants on surface tissue of postvaccinal PDA plating medium, this white colony changed in the PDA slant medium save backup;
E. from the PDA slant medium that changes white colony over to, get the Huang bacterium that withers, change in the PDA plating medium, carry out 27 ℃ of constant temperature culture 2-3 weeks, obtain the cultured Huang bacterium bacterium piece that withers;
F. the bacterium bacterium piece that cultured Huang withered vibrates in sterile water, filters, and utilizes blood counting chamber to adjust spore concentration, obtains the Huang bacterium spore suspension that withers;
D, pathogen infection are processed:
A. two leaves seedling is wholeheartedly extracted from described culture medium for seedling, carried out cleaning treatment, hinder again root and process, obtain hindering the seedling behind the root;
The seedling that b. will hinder behind the root is infected processing, obtains infecting seedling;
E, incidence survey:
Respectively at 5 days, 10 days, 15 days, 20 days that infect after the processing, investigate the described onset state that infects seedling, according to eggplant verticillium wilt severity Scaling standard 0,1,2,3,4 totally 5 the morbidity grades, according to this morbidity rating calculation disease index, judge again the eggplant verticillium wilt resistance classification of described Eggplant Germplasm Resources according to this disease index again.
2. the rapid identification method of described eggplant verticillium wilt resistance according to claim 1, it is characterized in that: in the step e, eggplant verticillium wilt severity Scaling standard is:
0 grade: without diseased plant; 1 grade: complete stool yellow wilting blade is less than 1/4; 2 grades: complete stool yellow wilting blade is more than or equal to 1/4 and less than 1/2; 3 grades: complete stool yellow wilting blade accounts for more than or equal to 1/2 and less than 3/4; 4 grades: complete stool yellow wilting blade reaches withered to all wilting more than 3/4.
3. the rapid identification method of described eggplant verticillium wilt resistance according to claim 1, it is characterized in that: in the step e, the disease index account form is:
Disease index DI=∑ (progression * strain number)/(highest number * total strain number) * 100;
Eggplant verticillium wilt resistance grade scale is as follows:
Disease-resistant (R): DI≤15; In anti-(MR): 15＜DI≤30; Anti-disease (T): 30＜DI≤50; Middle sense (MS): 50＜DI≤70; Susceptible (S): DI＞70.
4. the rapid identification method of described eggplant verticillium wilt resistance according to claim 1, it is characterized in that: Eggplant Germplasm Resources comprises distant eggplant No. 6, the green eggplant in Xi'an, distant eggplant No. 3, distant eggplant No. 5, Tianjin soon circle and Solanum Torvum in the steps A.
5. the rapid identification method of described eggplant verticillium wilt resistance according to claim 1 is characterized in that: the position that the pathogen of step C-a may comparatively dense is stem foot, cane, petiole.
6. the rapid identification method of described eggplant verticillium wilt resistance according to claim 1, it is characterized in that: the preparation method of the PDA plating medium among the step C-d is: peeling potato 200 g, be cut into the approximately fritter of 1.5 cm of the length of side, add 1000mL water boil 30 min, with 4 layers of filtered through gauze, in filtrate, add glucose 20 g and agarose 15 g, supply again water to 1000 mL after the heating for dissolving; The pH nature, 121 ℃, 30 min sterilization; Be down flat plate at superclean bench afterwards, obtain the PDA plating medium.
7. the rapid identification method of described eggplant verticillium wilt resistance according to claim 1 is characterized in that: the Huang of the step C-f bacterium spore suspension spore concentration that withers is 0.8-1.5 * 10
7Individual/mL.
8. the rapid identification method of described eggplant verticillium wilt resistance according to claim 1, it is characterized in that: the root of hindering of step D-a is treated to: wiping out length is the main root tip of a root and the corresponding fibrous root of 1.5-2.5cm.
9. the rapid identification method of described eggplant verticillium wilt resistance according to claim 1 is characterized in that: infecting processing among the step D-b, is that the eggplant seedling of will hinder behind the root is placed in the verticillium wilt spore suspension, blocks vessel port with aseptic gas permeable material; Be positioned over greenhouse by solar heat, environmental temperature is 25-30 ℃ of daytime again, 15-20 ℃ of night.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102646752A CN103355025A (en) | 2013-06-27 | 2013-06-27 | Method for rapidly identifying resistance to verticillium wilt of eggplant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102646752A CN103355025A (en) | 2013-06-27 | 2013-06-27 | Method for rapidly identifying resistance to verticillium wilt of eggplant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103355025A true CN103355025A (en) | 2013-10-23 |
Family
ID=49357984
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013102646752A Pending CN103355025A (en) | 2013-06-27 | 2013-06-27 | Method for rapidly identifying resistance to verticillium wilt of eggplant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103355025A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931432A (en) * | 2014-04-21 | 2014-07-23 | 云南省农业科学院园艺作物研究所 | Inoculation method for eggplant greensickness |
| CN104152534A (en) * | 2014-08-08 | 2014-11-19 | 四川省农业科学院经济作物育种栽培研究所 | Method for quickly measuring resistance of eggplant verticillium wilt at seedling stage |
| CN104480189A (en) * | 2014-11-21 | 2015-04-01 | 商丘师范学院 | In vitro identification method for tomato resistance to verticillium wilt |
| CN105145150A (en) * | 2015-08-04 | 2015-12-16 | 新疆农垦科学院 | Plant disease resistance evaluation method, root injuring method and inoculation method |
| CN105409613A (en) * | 2015-12-05 | 2016-03-23 | 武汉市蔬菜科学研究所 | Seedling-stage root rot resistance identification method for vigna unguiculata |
| CN106105820A (en) * | 2016-08-08 | 2016-11-16 | 内蒙古自治区农牧业科学院 | The method of indoor Rapid identification Helianthi in seedling stage resistance to verticillium wilt |
| CN106967783A (en) * | 2017-02-23 | 2017-07-21 | 河南省农业科学院芝麻研究中心 | A kind of method of characterization and evaluation sesame fusarium wilt disease resistance level |
| CN108377893A (en) * | 2018-02-13 | 2018-08-10 | 东北农业大学 | A kind of rapid detection method of eggplant verticillium wilt disease resistance |
| CN108718771A (en) * | 2018-06-04 | 2018-11-02 | 黑龙江省农业科学院园艺分院 | It is a kind of to hinder root inocalation method progress eggplant verticillium wilt Resistance Identification method using bottomless alms bowl |
| CN109197423A (en) * | 2018-10-30 | 2019-01-15 | 山东省农业科学院蔬菜花卉研究所 | A kind of cuttage and seedling culture identification method of melon powdery mildew resistance |
| CN110338055A (en) * | 2019-05-17 | 2019-10-18 | 扬州大学 | A kind of eggplant anti-blight rapid identification method |
| CN111121629A (en) * | 2019-12-31 | 2020-05-08 | 广西壮族自治区农业科学院 | Soybean leaf wrinkling field identification method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1678253A1 (en) * | 1989-09-13 | 1991-09-23 | Научно-производственное объединение "Элита Поволжья" | Method for evaluation of alfalfa resistance to diseases |
| CN102732596A (en) * | 2011-04-01 | 2012-10-17 | 华中农业大学 | Cotton verticillium wilt resistant seedling stage identification method |
-
2013
- 2013-06-27 CN CN2013102646752A patent/CN103355025A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1678253A1 (en) * | 1989-09-13 | 1991-09-23 | Научно-производственное объединение "Элита Поволжья" | Method for evaluation of alfalfa resistance to diseases |
| CN102732596A (en) * | 2011-04-01 | 2012-10-17 | 华中农业大学 | Cotton verticillium wilt resistant seedling stage identification method |
Non-Patent Citations (7)
| Title |
|---|
| 周宝利等: "不同品种茄子抗黄萎病特性与生理生化指标及根际微环境指标的相关性分析", 《沈阳农业大学学报》 * |
| 周宝利等: "不同抗病性茄子根系分泌物对黄萎菌的化感作用", 《生态学报》 * |
| 宋敏丽: "茄子砧木黄萎病抗性的苗期室内接种鉴定", 《太原师范学院学报》 * |
| 易金鑫等: "茄子种质资源抗黄萎病性评估", 《江苏农业科学》 * |
| 曾华兰等: "茄子品种资源抗黄萎病性鉴定评价", 《西南农业学报》 * |
| 李海涛等: "茄子黄萎病抗病性鉴定", 《辽宁农业科学》 * |
| 肖蕴华等: "茄子种质资源黄萎病抗性鉴定", 《中国蔬菜》 * |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931432B (en) * | 2014-04-21 | 2016-01-20 | 云南省农业科学院园艺作物研究所 | A kind of inoculation method of eggplant verticillium wilt |
| CN103931432A (en) * | 2014-04-21 | 2014-07-23 | 云南省农业科学院园艺作物研究所 | Inoculation method for eggplant greensickness |
| CN104152534A (en) * | 2014-08-08 | 2014-11-19 | 四川省农业科学院经济作物育种栽培研究所 | Method for quickly measuring resistance of eggplant verticillium wilt at seedling stage |
| CN104152534B (en) * | 2014-08-08 | 2016-05-04 | 四川省农业科学院经济作物育种栽培研究所 | A kind of eggplant verticillium wilt quick Bioassay method in seedling stage |
| CN104480189A (en) * | 2014-11-21 | 2015-04-01 | 商丘师范学院 | In vitro identification method for tomato resistance to verticillium wilt |
| CN105145150B (en) * | 2015-08-04 | 2018-11-09 | 新疆农垦科学院 | Disease resistance of plant identification method hinders method for root and inoculation method |
| CN105145150A (en) * | 2015-08-04 | 2015-12-16 | 新疆农垦科学院 | Plant disease resistance evaluation method, root injuring method and inoculation method |
| CN105409613A (en) * | 2015-12-05 | 2016-03-23 | 武汉市蔬菜科学研究所 | Seedling-stage root rot resistance identification method for vigna unguiculata |
| CN106105820A (en) * | 2016-08-08 | 2016-11-16 | 内蒙古自治区农牧业科学院 | The method of indoor Rapid identification Helianthi in seedling stage resistance to verticillium wilt |
| CN106967783A (en) * | 2017-02-23 | 2017-07-21 | 河南省农业科学院芝麻研究中心 | A kind of method of characterization and evaluation sesame fusarium wilt disease resistance level |
| CN106967783B (en) * | 2017-02-23 | 2020-05-19 | 河南省农业科学院芝麻研究中心 | Method for identifying and evaluating resistance level of sesame wilt disease |
| CN108377893A (en) * | 2018-02-13 | 2018-08-10 | 东北农业大学 | A kind of rapid detection method of eggplant verticillium wilt disease resistance |
| CN108718771A (en) * | 2018-06-04 | 2018-11-02 | 黑龙江省农业科学院园艺分院 | It is a kind of to hinder root inocalation method progress eggplant verticillium wilt Resistance Identification method using bottomless alms bowl |
| CN109197423A (en) * | 2018-10-30 | 2019-01-15 | 山东省农业科学院蔬菜花卉研究所 | A kind of cuttage and seedling culture identification method of melon powdery mildew resistance |
| CN109197423B (en) * | 2018-10-30 | 2020-10-02 | 山东省农业科学院蔬菜花卉研究所 | Cutting seedling identification method for melon powdery mildew resistance |
| CN110338055A (en) * | 2019-05-17 | 2019-10-18 | 扬州大学 | A kind of eggplant anti-blight rapid identification method |
| CN111121629A (en) * | 2019-12-31 | 2020-05-08 | 广西壮族自治区农业科学院 | Soybean leaf wrinkling field identification method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103355025A (en) | Method for rapidly identifying resistance to verticillium wilt of eggplant | |
| CN103477921B (en) | Method for comprehensive control of root knot nematode disease of potted ficus microcarpa | |
| CN104160846B (en) | A kind of sprout period testifying method of cowpea anti-blight | |
| CN104928191B (en) | For herbaceous plant diseases prevention, growth-promoting, degeneration-resistant arbuscular mycorrhizal fungi GZ176 and its microbial inoculum and application | |
| CN105409613A (en) | Seedling-stage root rot resistance identification method for vigna unguiculata | |
| CN100484391C (en) | Method for identifying bacterial wilt resistivity of solanaceous crops | |
| CN103937720B (en) | The nematicide albumen of one strain wax printing fabric and secretion thereof | |
| CN105340545A (en) | Seedling stage puncture inoculation method for fusarium oxysporum f. sp niveum resistance | |
| CN101849469A (en) | Method for identifying disease resistance of watermelon seedling stage | |
| CN105018566B (en) | A kind of sweet potato brown spot Resistance Identification method | |
| CN102061330B (en) | Method for identifying pathogenicity of sesame stem blight and blast pathogenic bacteria | |
| CN102154193B (en) | Method for inducing Phytophthora capsici to produce large amount of sporangia | |
| CN103305438A (en) | Biocontrol strain Jdm2 for preventing and treating tomato root-knot nematode diseases and antibiological inoculant thereof | |
| CN107667738A (en) | The method of seedling stage assay watermelon blight resistance | |
| CN102876584B (en) | Xylaria strain and application thereof | |
| Lichtenzveig et al. | Inoculation and growth with soil borne pathogenic fungi | |
| CN108085363A (en) | One identification method to grow tobacco to fusarium tabacinum Resistance To Root Rot Disease | |
| CN105112304A (en) | Bjerkandera sp. Gause 15 for controlling vegetable root diseases and preparation thereof | |
| CN117603843A (en) | Sword adhesion bacteria for preventing and treating plant root knot nematode disease, fermentation liquor and application thereof | |
| Tamur et al. | Marasmiellus palmivorus as a new causal agent of reed wilt disease in Iraq | |
| CN111296278A (en) | Method for breeding clubroot-resistant Chinese cabbages | |
| CN113999788B (en) | Actinomycetes and application thereof | |
| CN110317735A (en) | Biological and ecological methods to prevent plant disease, pests, and erosion pythium oligadrum and application | |
| CN102382776B (en) | Small spore phoma microspora for controlling conyza sumatrensis | |
| CN110249962A (en) | Resistance Identification method in sweet potato root rot seedling stage room |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20131023 |